CN115734818A - Detection chip, detection device and method for preparing and operating detection chip - Google Patents
Detection chip, detection device and method for preparing and operating detection chip Download PDFInfo
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- CN115734818A CN115734818A CN202080000374.7A CN202080000374A CN115734818A CN 115734818 A CN115734818 A CN 115734818A CN 202080000374 A CN202080000374 A CN 202080000374A CN 115734818 A CN115734818 A CN 115734818A
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Abstract
A test chip (100), a test device and methods of making and operating a test chip (100). The detection chip (100) includes: a substrate (110), the substrate (110) comprising a first surface (111); wherein the substrate (110) comprises a reservoir (112), the reservoir (112) being configured to contain a liquid and comprising a bottom portion on the first surface (111), the bottom portion comprising a central region (113) and an edge region surrounding the central region (113), the edge region comprising a channel (114/114 ') surrounding the central region, the channel (114/114') having a thickness being smaller than a thickness of the central region (113). The detection chip (100) is convenient to release the liquid stored in the detection chip, and has simple structure and manufacturing process and low cost.
Description
Embodiments of the present disclosure relate to a detection chip, a detection device, and methods of preparing and operating the detection chip.
The microfluidic chip is also called a Lab-on-a-chip (Lab-on-a-chip), and is characterized in that basic operation units related to the fields of biology, chemistry, medicine and the like, such as sample preparation, reaction, separation, detection and the like, are integrated on a chip with a micro-channel with a micron scale, and the whole process of reaction and analysis is automatically completed. The analysis and detection device based on the microfluidic chip has the advantages of less sample consumption, high analysis speed, convenient manufacture into a portable instrument and suitability for real-time and on-site analysis.
Disclosure of Invention
At least one embodiment of the present disclosure provides a detection chip, including:
a substrate comprising a first surface;
wherein the substrate includes a reservoir configured to contain a liquid and including a bottom portion on the first surface,
the bottom portion includes a central region and an edge region surrounding the central region,
the edge regions comprise a channel surrounding the central region, the channel having a thickness less than a thickness of the central region.
For example, in a detection chip according to at least one embodiment of the present disclosure, the channel is a non-enclosed channel to partially surround the central region.
For example, in a detection chip according to at least one embodiment of the present disclosure, the channel is a closed channel to surround the central region by one circle.
For example, in a detection chip according to at least one embodiment of the present disclosure, the liquid storage chamber has a cylindrical shape, and the channel has a substantially annular shape.
For example, in the detection chip according to at least one embodiment of the present disclosure, the central angle corresponding to the channel is 340 to 358 degrees.
For example, in a detection chip according to at least one embodiment of the present disclosure, the thickness of the central region is 0.1mm to 3mm.
For example, in the detection chip according to at least one embodiment of the present disclosure, the thickness of the channel is 0.05mm to 0.3mm, and the width of the channel is 0.1mm to 3mm.
For example, in a detection chip according to at least one embodiment of the present disclosure, a face of the bottom portion facing the inner space of the reservoir is a plane, and the channel is recessed at the first surface facing the inner space of the reservoir.
For example, in a detection chip according to at least one embodiment of the present disclosure, a face of the bottom portion facing away from the inner space of the reservoir is a plane, and the channel is recessed away from the inner space of the reservoir at the face of the bottom portion facing toward the inner space of the reservoir.
For example, in a detection chip according to at least one embodiment of the present disclosure, the reservoir further comprises a sidewall, the channel is between the sidewall and the central region,
the edge region further comprises a connecting portion,
wherein the connection is between the sidewall and the central region, and the connection corresponds to an opening portion of the channel that does not surround the central region.
For example, in a detection chip according to at least one embodiment of the present disclosure, the connection portion is configured to allow the central region to make an angle greater than zero degrees and less than 25 degrees with the side wall in a case where the channel is broken.
For example, in a detection chip according to at least one embodiment of the present disclosure, the substrate further includes a liquid flow channel on the first surface,
wherein the liquid flow channel is communicated with the channel.
For example, in a detection chip according to at least one embodiment of the present disclosure, on the first surface, the connection portion and the liquid flow channel are located on opposite sides of the central region.
For example, the detection chip according to at least one embodiment of the present disclosure further includes: a first elastic film layer, a second elastic film layer,
wherein the liquid flow channel includes a groove on the first surface of the substrate,
the first elastic film layer is arranged on the first surface of the substrate, covers the bottom and covers the groove to provide a liquid flowing space.
For example, a detection chip according to at least one embodiment of the present disclosure further includes a second elastic film layer,
the substrate further comprises a second surface opposite the first surface,
the second elastic film layer is arranged on the second surface of the substrate and covers the opening of the liquid storage chamber on the second surface.
For example, the detection chip according to at least one embodiment of the present disclosure further includes: a first adhesive layer and a second adhesive layer,
wherein the first adhesive layer is between the substrate and the first elastic film layer to connect the substrate and the first elastic film layer; and
the second adhesive layer is between the substrate and the second elastic film layer to connect the substrate and the second elastic film layer.
For example, a detection chip according to at least one embodiment of the present disclosure further includes a liquid manipulation region,
the liquid handling zone is in communication with the liquid flow channel.
For example, in a detection chip according to at least one embodiment of the present disclosure, the liquid handling zone includes at least one selected from the group consisting of a liquid mixing zone, a liquid detection zone, and a liquid storage zone.
At least one embodiment of the present disclosure also provides a detection apparatus, including:
the detection chip provided by any embodiment of the disclosure; and
a force application mechanism configured to, in use, apply a force on the bottom portion of the detection chip towards the interior space of the reservoir to break the channel and cause liquid contained within the interior space to flow out of the bottom portion.
At least one embodiment of the present disclosure also provides a method for preparing a detection chip, including:
providing a substrate, the substrate comprising a first surface,
wherein the substrate includes a reservoir configured to contain a liquid and including a bottom portion at the first surface,
the bottom portion includes a central region and an edge region surrounding the central region, an
The edge regions include a channel surrounding the central region, the channel having a thickness less than a thickness of the central region.
For example, the method for preparing the detection chip according to at least one embodiment of the present disclosure further includes: the substrate is prepared by an integral molding method.
At least one embodiment of the present disclosure further provides a method for operating a detection chip provided in any one of the embodiments of the present disclosure, which includes:
applying a force on the bottom of the reservoir toward an interior space of the reservoir to break the channel and allow liquid contained within the interior space to flow out of the bottom.
At least one embodiment of the present disclosure also provides a method for operating a detection chip provided in at least one embodiment of the present disclosure, which includes:
applying a force to the bottom of the reservoir at a location on the first resilient film layer corresponding to a location 0-3/4 of the length of the central region from the connection to break the channel and allow liquid contained within the interior space of the reservoir to flow out of the bottom.
To more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below, and it is apparent that the drawings described below only relate to some embodiments of the present disclosure and do not limit the present disclosure.
Fig. 1 is a schematic perspective view of a detection chip according to at least one embodiment of the present disclosure.
Fig. 2 is a bottom perspective view of a reservoir of the detection chip shown in fig. 1 according to at least one embodiment of the present disclosure.
Fig. 3 is a bottom plan view of the reservoir of the detection chip shown in fig. 1, in accordance with at least one embodiment of the present disclosure.
Fig. 4 isbase:Sub>A perspective cross-sectional view along linebase:Sub>A-base:Sub>A' in fig. 2 according to at least one embodiment of the present disclosure.
Fig. 5 is a cross-sectional view along line B-B' in fig. 2 according to at least one embodiment of the present disclosure.
Fig. 6 is another cross-sectional view along line B-B' in fig. 2 according to at least one embodiment of the present disclosure.
Fig. 7A and 7B are sectional views along linebase:Sub>A-base:Sub>A' in fig. 2 illustratingbase:Sub>A liquid releasing process according to at least one embodiment of the present disclosure.
Fig. 8 is an exploded schematic view of another detection chip according to at least one embodiment of the present disclosure.
Fig. 9 is an assembly diagram of the detection chip shown in fig. 8.
Fig. 10 is a schematic perspective view of the substrate in fig. 8 in accordance with at least one embodiment of the present disclosure.
Fig. 11 is a bottom perspective view of the base plate of fig. 8 according to at least one embodiment of the present disclosure.
Fig. 12A and 12B are cross-sectional views along linebase:Sub>A-base:Sub>A' in fig. 9 illustratingbase:Sub>A liquid releasing process according to at least one embodiment of the present disclosure.
Fig. 13 is a schematic block diagram of a detection apparatus in accordance with at least one embodiment of the present disclosure.
In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be described below clearly and completely with reference to the accompanying drawings. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without inventive step, are within the scope of protection of the disclosure.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and the like in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. Likewise, the word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
It is understood that in at least one embodiment of the present disclosure, "substantially identical" means that the difference between two objects is within 5% to-5% of the object being compared.
The reagent storage structure in the existing microfluidic chip is complex in structure or needs a complex preparation process, so that the cost of the microfluidic chip as a consumable material is too high. For example, in some microfluidic chips, the bottom of the reservoir is packaged by an aluminum film or a plastic film, so that when the aluminum film or the plastic film is punctured to release the reagent stored in the reservoir, the broken edge portion protrudes upward, which easily causes the reagent to remain, and all the reagents in the reservoir cannot be completely released, so that the reagent cannot be added in a sufficient amount during the test, and the biological reaction result is abnormal. In addition, in these microfluidic chips, the liquid stored in the liquid reservoir is easily leaked, and a hot pressing, a laser welding, and the like are also required, resulting in a complicated process and high cost.
In view of at least one of the above problems, at least one embodiment of the present disclosure provides a detection chip that facilitates release of a liquid stored therein, and that is simple in structure and manufacturing process and low in cost.
The detection chip provided by the embodiments of the present disclosure may be a microfluidic chip, however, it should be understood that the embodiments of the present disclosure are not limited thereto.
In addition, the embodiment of the disclosure also provides a detection device comprising any detection chip and a method for preparing and operating any detection chip.
Fig. 1 is a schematic perspective view of a detection chip according to at least one embodiment of the present disclosure. Fig. 2 is a bottom perspective view of a reservoir of the detection chip shown in fig. 1 according to at least one embodiment of the present disclosure. Fig. 3 is a bottom plan view of the reservoir of the detection chip shown in fig. 1, in accordance with at least one embodiment of the present disclosure. Fig. 4 isbase:Sub>A cross-sectional view of at least one embodiment of the present disclosure along linebase:Sub>A-base:Sub>A' in fig. 2. Fig. 5 is a cross-sectional view along line B-B' in fig. 2, in accordance with at least one embodiment of the present disclosure. Fig. 6 is another cross-sectional view along line B-B' in fig. 2 according to at least one embodiment of the present disclosure.
As shown in fig. 1-6, a detection chip 100 according to at least one embodiment of the present disclosure includes a substrate 110. The substrate 110 includes a first surface 111 and a second surface 111' opposite to the first surface 111. The substrate 110 also includes a reservoir 112. The reservoir 112 is configured to contain a liquid (e.g., various reagents required for analytical testing) and is included at the bottom of the first surface 111. The bottom portion includes a central region 113 and an edge region surrounding the central region 113. The edge region includes channels 114/114'/114 "surrounding the central region 113, and the thickness h1 of the channels 114/114'/114" is less than the thickness h2 of the central region 113. It should be understood that reference herein to the thickness of the channels 114/114'/114 "is to the thickness of the bottom of the reservoir 112 at the channels 114/114'/114", and the thickness of the central region 113 is to the thickness of the bottom of the reservoir 112 at the central region 113.
Since the thickness h1 of the channels 114/114'/114 "is less than the thickness h2 of the central region 113, when a force is applied toward the interior of the reservoir 112 at a location corresponding to the reservoir 112 (e.g., at the central region 113) on the first surface 111, the channels 114/114'/114" are more likely to be broken with respect to the central region 113, thereby forming an opening along the path of the channel 114/114'/114 ″ to release the liquid contained in the liquid storage chamber 112 from the liquid storage chamber 112, for example, to an external device (e.g., other analysis detection device, etc.) connected to the detection chip 100 or to a liquid flow path in the detection chip 100. For example, when a force is applied on the first surface 111 toward the interior of the reservoir 112 at a location corresponding to the reservoir 112 (e.g., at the central region 113), the bottom of the reservoir 112 splits along the channels 114/114'/114 "such that the edge of the central region 113 surrounded by the channels 114 is separated from the rest of the bottom of the reservoir 112, and continuing to apply the force on the central region 113 may cause the central region 113 to continue to move in a direction away from the first surface 111 (e.g., to tilt relative to the first surface 111), forming an opening between the central region 113 and the rest of the bottom of the reservoir 112 to release the liquid contained within the reservoir 112 from the reservoir 112. In this way, the problem of the other detection chips described above that all reagents in the reservoirs cannot be completely released can be avoided.
Herein, the thickness refers to a thickness in a direction perpendicular to the first surface 111. It should be understood that where the channel 114/114'/114 "has the same thickness throughout the perimeter, reference herein to the thickness of the channel 114/114'/114" may refer to the thickness at any one of the channels 114/114'/114 "; in the case where the channels 114/114'/114 "have different thicknesses throughout the perimeter, reference herein to the thickness of the channels 114/114'/114" is made to the maximum thickness of the channels 114/114'/114 ". Similarly, where the central region 113 has the same thickness throughout the region, reference herein to the thickness of the central region 113 may refer to the thickness at any point of the central region 113; in the case where the central region 113 has different thicknesses in the entire region, the thickness of the central region 113 mentioned herein refers to the minimum thickness of the central region 113. Hereinafter, embodiments of the present disclosure will be described by taking as an example that the central region 113 has the same thickness over the entire region and the channel 114 has the same thickness over the entire circumference.
The material of the substrate 110 may be any suitable material according to actual requirements, for example, glass, silicon, quartz, ceramic, polyethylene Terephthalate (PET), polystyrene (PS), polymethyl methacrylate (PMMA), polypropylene (PP), polycarbonate (PC), or a combination thereof, which is not limited in this disclosure. For example, when the detection chip 100 is used for immunoassay, the material of the substrate 110 may be PS or PMMA; when the detection chip 100 is used for molecular detection, the material of the substrate 110 may be PP or PC.
Although only one reservoir is shown in fig. 1-6 for the substrate 110, embodiments of the present disclosure are not limited thereto, and in other embodiments, the substrate 110 may include any number of reservoirs that may contain various reagents required for an analytical test, and that may have the same or different shapes and may contain the same or different liquids, depending on the actual requirements.
As shown in fig. 2, in at least one embodiment of the present disclosure, the channel 114 is a non-enclosed channel to partially surround the central region 113. For example, the channel 114 may be substantially C-shaped, U-shaped, and the like.
However, it should be understood that in other embodiments, as shown in fig. 3, the channel 114' may be a closed channel to surround the central region 113 a full turn. For example, the channel 114' may be circular, polygonal (e.g., quadrilateral, etc.).
Illustratively, the reservoir 112 may be cylindrical with a bottom surface parallel to the first surface 111, and the channels 114/114'/114 "may be substantially annular. However, it should be understood that in other embodiments, the reservoir 112 and the channels 114/114'/114 ″ may also have any suitable shape, as desired, and embodiments of the present disclosure are not limited in this respect. Hereinafter, embodiments of the present disclosure will be described by taking the example that the reservoir 112 has a cylindrical shape and the channel 114 has a circular ring shape.
In some embodiments, the central angle of the channel may be 330-360 degrees. For example, the channel 114 may have a central angle of 340-358 degrees. Illustratively, the central angle of the channel 114 may be 340 degrees, 345 degrees, 350 degrees, 355 degrees, or 358 degrees. For example, the channel 114 may correspond to a central angle of 350 degrees to facilitate breaking the channel 114, thereby breaking open an opening along the path of the channel 114 to completely release the liquid contained within the reservoir 112 from the reservoir 112 and prevent the channel 114 from being inadvertently broken during non-use, such as transportation, storage, and the like.
In some embodiments, the thickness of the central region 113 may be, for example, 0.1mm to 3mm. Illustratively, the thickness of the central region 113 may be 0.1mm, 0.5mm, 1mm, 1.5mm, 2mm, 2.5mm, or 3mm.
In some embodiments, the thickness of the channel 114 may be, for example, 0.05mm to 0.3mm for the above materials. Illustratively, the thickness of the channel 114 may be 0.05mm, 0.1mm, 0.15mm, 0.2mm, 0.25mm, or 0.3mm.
In some embodiments, the width of the channel 114 is 0.1mm to 3mm. The width mentioned here refers to a width in a direction parallel to the first surface 111. Illustratively, the width of the channel 114 may be 0.1mm, 0.5mm, 1mm, 1.5mm, 2mm, 2.5mm, or 3mm, such that the channel 114 is easily broken to release the liquid contained in the reservoir 112 from the reservoir 112.
In one exemplary embodiment, the channel 114 corresponds to a central angle of 350 degrees, the channel 114 has a width of 1mm, the channel 114 has a thickness of 0.1mm, and the central region 113 has a thickness of 1.5mm. This may allow the channel 114 to be easily broken to release the liquid contained in the reservoir 112 from the reservoir 112.
As shown in fig. 4 and 5, in some embodiments, a face of the bottom of the reservoir 112 facing the interior space of the reservoir 112 is planar, and the channel 114 is recessed at the first surface 111 facing the interior space of the reservoir 112. In other embodiments, as shown in fig. 6, the bottom of the reservoir 112 is planar toward the first surface 111, and the channel 114 "is recessed toward the first surface 111 at the inner bottom surface of the reservoir 112. Embodiments of the present disclosure are not limited in this regard. Hereinafter, description will be made taking an example in which the substrate 110 includes a channel 114 recessed at the first surface 111 toward the inner space of the liquid reservoir 112.
In some embodiments, the reservoir 112 also includes a sidewall 116, with the channel 114 between the sidewall 116 and the central region 113. As shown in fig. 4, the channel 114 may be, for example, in direct contact with the sidewall 116; as shown in fig. 5-7B, the channel 114 may also be spaced apart from the sidewall 116, for example, and embodiments of the present disclosure are not limited in this regard.
The edge region may also include a connection 117. The connection 117 is between the sidewall 116 and the central region 113, and the connection 117 corresponds to an opening portion of the channel 114 that does not surround the central region 113. For example, the channel 114 and the connection 117 may together surround the central region 113 a full turn. Of course, it should be understood that where the substrate 110 includes a closed channel 114' as shown in fig. 3, the connection 117 may not be present. Embodiments of the present disclosure are not limited in this regard.
In some embodiments, the connection 117 is configured to remain substantially intact due to the thicker thickness, not to split, in the event that the channel 114 is broken to split to form an opening, thereby connecting between the central region 113 and the sidewall 116 and allowing the central region 113 to be angled away from the sidewall 116 when ejected. For example, the central region 113 may be configured to have a certain rigidity so as to be able to remain substantially undisturbed without bending when being pushed open, thereby avoiding blocking the liquid in the reservoir 112 from flowing out of the opening formed by the channel 114 splitting open.
For example, in the case where the central angle corresponding to the channel 114 is 350 degrees, when a force is applied to the inside of the reservoir 112 at a position corresponding to the reservoir 112 (for example, at the central region 113) on the first surface 111, the size of the opening formed by the channel 114 being split is substantially the same as the size of the cross section of the reservoir 112, and the central region 113 remains connected to the substrate 110 through the connecting portion 117, and the force is continuously applied to the central region 113, so that an angle greater than 0 degrees and less than 90 degrees, for example, an angle of 10 degrees to 25 degrees, is formed between the central region 113 and the side wall 116, thereby completely releasing the liquid contained in the reservoir 112 from the reservoir 112, and since the central region 113 remains connected to the substrate 110 through the connecting portion 117, the problem that if the central region 113 is completely separated from the substrate 110, the central region 113 becomes horizontal as the liquid in the reservoir 112 flows and blocks the opening is avoided.
In some embodiments, the connection 117 is configured to allow the central region 113 to be at an angle greater than 0 degrees and less than 90 degrees, such as greater than 0 degrees and less than 25 degrees, with the sidewall 116 to facilitate the entire release of liquid within the reservoir 112. For example, the central angle of the connecting portion 117 may be 2 to 20 degrees. Illustratively, the central angle of the connecting portion 117 is 10 degrees.
For example, when a force is applied to the interior of the reservoir 112 at a location on the first surface 111 corresponding to the reservoir 112 (e.g., at the central region 113), the coupling 117 flexes such that the central region 113 may be at an angle greater than 0 degrees and less than 90 degrees, such as an angle of 10 degrees-25 degrees, with the side walls 116, and the flexing of the coupling 117 may be plastically deformed at this time, thereby causing the central region 113 to maintain the angle with the side walls 116 after the force is withdrawn, or the flexing of the coupling 117 may be elastically deformed at this time, thereby requiring the force to be applied at all times to maintain the angle between the central region 113 and the side walls 116, such as until the liquid within the reservoir 112 is completely released.
Fig. 7A and 7B are cross-sectional views taken along linebase:Sub>A-base:Sub>A' in fig. 2 ofbase:Sub>A liquid discharge process according to at least one embodiment of the present disclosure, and fig. 7A and 7B illustratebase:Sub>A schematic view of the liquid discharge process of the detection chip shown in fig. 2. As shown in fig. 7A and 7B, in the event that a force is applied in the direction indicated by arrow a toward the bottom of the reservoir 112 (e.g., the central region 113), the channel 114 may be broken, the bottom of the reservoir 112 may be split along the channel 114, the edge of the central region 113 surrounded by the channel 114 may be separated from the rest of the bottom of the reservoir 112, the connection 117 connects the central region 113 with the sidewall 116, and the connection 117 allows the angle θ between the central region 113 and the sidewall 116 to be an angle greater than 0 degrees and less than 90 degrees, such as an angle of 10 degrees to 25 degrees, to facilitate the liquid within the reservoir 112 to be completely released. Although the sidewall 116 is shown in fig. 4, 7A, and 7B as being substantially perpendicular to the first surface 111, it should be understood that embodiments of the present disclosure are not so limited, and in other embodiments, the sidewall 116 may also be oblique with respect to the first surface 111, or the sidewall 116 may have a curved surface.
In some embodiments, the thickness of the connection portion 117 may be substantially the same as the thickness of the central region 113 in a direction perpendicular to the first surface 111 to simplify the manufacturing process, however, it is understood that embodiments of the present disclosure are not limited thereto. For example, in other embodiments, the thickness of the connection portion 117 may be less than the thickness of the central region 113. Furthermore, the thickness of the connection 117 is greater than the thickness of the channel 114, so that in case the channel 114 is damaged, the connection 117 can still connect with the central region 113 and the side walls 116.
It should be understood that, in at least one embodiment of the present disclosure, the thickness of the connection portion 117 may be substantially the same as the thickness of the central region 113 may mean that the thickness of the connection portion 117 and the thickness of the central region 113 are the same, substantially the same, or different within a preset range, for example, the difference between the thickness of the connection portion 117 and the thickness of the central region 113 is within 5% to-5% of the specific thickness.
Fig. 8 is an exploded schematic view of another detection chip according to at least one embodiment of the present disclosure. Fig. 9 is an assembly diagram of the detection chip shown in fig. 8. As shown in fig. 8, a detection chip 200 according to at least one embodiment of the present disclosure may include a substrate 210. The substrate 210 is substantially the same as the substrate 110 described in any of the embodiments above, except that the substrate 210 further includes a liquid channel 215 on the first surface 211. For the description of the same or similar portions of the substrate 210 and the substrate 110, reference may be made to the above detailed description of the embodiments, which will not be repeated herein.
Fig. 10 is a schematic perspective view of the substrate of fig. 8 in accordance with at least one embodiment of the present disclosure. Fig. 11 is a bottom perspective view of the base plate of fig. 8 according to at least one embodiment of the present disclosure. As shown in fig. 10-11, in at least one embodiment, the substrate 210 includes a liquid flow channel 215 on a first surface 211. The liquid flow channel 215 may include a groove on the first surface 211 of the substrate 210. For example, the groove may be formed by any suitable process, such as laser engraving, photolithography, or the like, or may be integrally formed with the substrate 210 during formation of the preparation substrate 210, which is not limited by the embodiments of the present disclosure. The liquid channel 215 is used to transport the liquid released from the liquid storage chamber 212 to other parts of the detection chip 200, such as the liquid operation area 219, including but not limited to a liquid mixing area, a liquid detection area, a liquid storage area, and a membrane pump area.
Where the bottom of the reservoir 212 includes a channel 214 recessed in the first surface 211 toward the interior space of the reservoir 112, the liquid flow channel 215 may be in communication with the channel 214.
In some embodiments, as shown in fig. 10-11, on the first surface 211, the connecting portion 217 is located on opposite sides of the central region 213 from the liquid channel 215 to facilitate the liquid released from the liquid reservoir 212 to enter the liquid channel 215. However, it should be understood that in other embodiments, the connection 217 and the liquid flow channel 215 may be arbitrarily positioned with respect to each other, as embodiments of the present disclosure are not limited in this regard. For example, the extending direction of the connection portion 217 may be 45 degrees, 90 degrees, 135 degrees, etc. to the extending direction of the liquid flow channel 215.
As shown in fig. 8, the detection chip 200 according to at least one embodiment of the present disclosure may further include a first elastic film layer 220 in addition to the substrate 210 described above. The substrate 210 includes a first surface 211 and a second surface 211' opposite the first surface 211. The first elastic film layer 220 is under the first surface 211 of the substrate 210 in a direction from the first surface 211 to the second surface 211'. For example, the first elastic film layer 220 may be in fluid tight connection with the substrate 210. The first resilient film layer 220 covers at least the bottom of the reservoir 212. In some embodiments, the first elastic film layer 220 covers the groove of the liquid flow channel 215 in addition to the bottom of the liquid storage chamber 212, thereby closing an open end surface of the groove of the liquid flow channel 215 parallel to the first surface 211 to provide a space for liquid flow, for example, to also form a space for reagent reaction. In some embodiments, the first elastic film layer 220 may also cover the entire first surface 211.
The first elastic film layer 220 has elasticity to allow elastic deformation. For example, where a force is applied on the first elastic film layer 220 toward the bottom of the reservoir 212 using, for example, a push rod, the first elastic film layer 220 can be elastically deformed to allow the push rod a certain travel such that the channels 214 are split and the central region 213 is at a desired angle (e.g., 10-25 degrees) to the sidewalls, and after the push rod is withdrawn to remove the force, the first elastic film layer 220 can substantially return to the original state without being damaged.
For example, the first resilient film layer 220 may be at least partially transparent, e.g., transparent or translucent, to allow viewing or optical detection of the liquid within the reservoir 212 and liquid flow channel 215.
For example, the material of the first elastic film layer 220 may be Polyethylene Terephthalate (PET), polystyrene (PS), polymethyl methacrylate (PMMA), polypropylene (PP), polycarbonate (PC), or the like to have good elasticity and strength, so that the initial state can be restored after elastic deformation. Of course, the embodiments of the present disclosure are not limited to the above materials, and the first elastic film layer 220 may also be made of other suitable materials, for example, a polymer composite material of PS and PET, so as to have better elasticity and strength.
Although in fig. 8, the first elastic film layer 220 is illustrated as having substantially the same contour shape as the first surface 211 of the substrate 210, it is to be understood that embodiments of the present disclosure are not limited thereto. For example, in some embodiments, the first elastic film layer 220 may cover only the bottom of the reservoir 212 and the recess of the liquid channel 215 is covered by another film layer (e.g., another elastic film layer or a rigid film layer) that is in sealing connection with the first elastic film layer 220 to prevent liquid leakage, or the first elastic film layer 220 may cover the bottom of the reservoir 212 and the recess of the liquid channel 215 with other portions of the first surface 212 being covered by the other film layer or no longer being covered and directly exposed to the outside environment.
In some embodiments, the detection chip 200 may further include a first adhesive layer 230. The first adhesive layer 230 is between the substrate 210 and the first elastic film layer 220 to connect the substrate 210 and the first elastic film layer 220.
For example, the first adhesive layer 230 may include a material having adhesive property such as an acrylic adhesive, and for example, may be implemented as an adhesive coating or as a double-sided tape. For example, the first adhesive layer 230 and the first elastic film layer 220 have substantially the same profile, whereby the first adhesive layer 230 may achieve a firm bond between the substrate 210 and the first elastic film layer 220.
In some embodiments, in the case where the first elastic film layer 220 covers the bottom of the liquid reservoir 212 and the groove of the liquid flow channel 215, the first adhesive layer 230 may be exposed on the first surface 211 of the substrate 210 and the bottom of the liquid reservoir 212. That is, the first adhesive layer 230 may include a hollowed-out region 231, and the shape of the hollowed-out region 231 is the same or substantially the same as the orthographic projection of the liquid channel 215 and the liquid storage chamber 212 on the first adhesive layer 230, so as to facilitate the first elastic film layer 220 and the liquid channel 215 to form a space for, for example, liquid flow and/or reagent reaction. In other embodiments, in the case that the first elastic film layer 220 only covers the bottom of the liquid storage chamber 212, the hollow area 231 of the first adhesive layer 230 may only expose the bottom of the liquid storage chamber 212.
In the case where the detection chip 200 includes the liquid handling region 219, the hollowed-out region 231 of the first adhesive layer 230 may include an opening 2311 corresponding to the liquid handling region 219 to provide a space for liquid flow and/or reagent reaction between the substrate 210 and the first elastic film layer 220. For example, the liquid handling zone 219 may be in communication with the liquid flow channel 215. It should be understood, however, that the shape and location of the liquid handling zone 219 shown in fig. 9 are exemplary and embodiments of the present disclosure are not limited in this regard.
Further, as shown in fig. 8, in some embodiments, the hollowed-out area 231 of the first adhesive layer 230 may further include a liquid flow control region 2312. The projection of the liquid flow control area 2312 on the first surface 211 falls on the extension line of the liquid flow channel 215. In operation, when pressed, manually or by an action device, on the first surface 211 at a location corresponding to the liquid flow control region 2312, the first elastic film layer 220 is elastically deformed so as to be abuttable against the substrate 210, thereby blocking the flow of liquid in the liquid flow channel 215, and when no longer pressed, the first elastic film layer 220 can be substantially restored to an original state without being broken, and the flow of liquid in the liquid flow channel 215 can be restored. This allows the flow of liquid in the liquid flow channel 215 to be controlled according to the actual requirements.
For example, in other embodiments, the first adhesive layer 230 may be omitted when the first elastic film layer 220 is bonded to the substrate 210 by using a thermal compression method, an ultrasonic welding method, a photo adhesive bonding method, a chemical solvent bonding method, or a laser welding method. For example, when the first elastic film layer 220 and the substrate 210 are formed of the same polymer material, the first elastic film layer 220 and the substrate 210 may be bonded by laser welding without providing the first adhesive layer 230.
In addition, as shown in fig. 8, the detection chip 200 according to at least one embodiment of the present disclosure may further include a second elastic film layer 240. The second elastic film layer 240 is over a second surface 211' of the substrate 210 opposite to the first surface 211 in a direction from the first surface 211 to the second surface 211', and covers at least the opening 218 of the liquid storage chamber 212 on the second surface 211'. In some embodiments, the second elastic film layer 240 may also cover the entire second surface 211'. The second elastic film layer 240 may be in fluid tight connection with the substrate 210 to prevent the liquid contained in the reservoir 212 from leaking out of the opening 218 on the second surface 211'.
The second elastic film layer 240 has elasticity to allow elastic deformation. For example, in the case where a force is applied to the reservoir 212 on the second elastic film layer 240 (e.g., by another action mechanism or manually), the second elastic film layer 240 can be elastically deformed to allow the liquid contained in the reservoir 212 to be squeezed (e.g., to cause the liquid contained in the reservoir 212 to flow out of the bottom of the reservoir 212), thereby allowing the liquid to flow out of the opening formed in the bottom, and after the force is removed, the second elastic film layer 240 can be substantially restored to the original state, e.g., the liquid contained in the reservoir 212 can be squeezed repeatedly many times to more sufficiently cause the liquid to flow out of the opening formed in the bottom. For example, the second elastic film layer 240 may be at least partially transparent, e.g., transparent or translucent, to allow viewing of the liquid within the reservoir 212 or optical detection.
For example, the material of the second elastic film layer 240 may be Polyethylene Terephthalate (PET), polystyrene (PS), poly (methyl methacrylate), PMMA), polypropylene (PP), polycarbonate (PC), or the like to have good elasticity and strength, so that the initial state can be restored after elastic deformation. Of course, the embodiments of the present disclosure are not limited thereto, and other suitable materials, for example, a polymer composite material of PS and PET, may be used for the second elastic film layer 240, so as to have better elasticity and strength.
The first and second elastic film layers 220 and 240 may be formed of the same or different materials, as embodiments of the present disclosure are not limited in this respect.
However, it should be understood that in the case where the reservoir 212 does not have the opening 218 on the second surface 211', the detection chip 200 may not include the second elastic film layer 240.
Although the second elastic film layer 240 is shown in fig. 8 to cover other portions of the second surface 211 'of the substrate 210 in addition to the opening 218 of the liquid storage chamber 212 on the second surface 211', it should be understood that embodiments of the present disclosure are not limited thereto. For example, in some embodiments, the second elastic film layer 240 may cover only the opening 218 of the reservoir 212 on the second surface 211'.
In some embodiments, the detection chip 200 may further include a second adhesive layer 250. The second adhesive layer 250 is between the substrate 210 and the second elastic film layer 240 to connect the substrate 210 and the second elastic film layer 240.
For example, the second adhesive layer 250 may include a material having adhesive property such as acrylic adhesive, and for example, may be implemented as an adhesive coating or as a double-sided tape. For example, the second adhesive layer 250 and the second elastic film layer 240 have substantially the same profile, whereby the second adhesive layer 250 may achieve a firm bond between the substrate 210 and the second elastic film layer 240.
In some embodiments, the second adhesive layer 250 is exposed at the opening 218 of the reservoir 212 on the second surface 211'. That is, the second adhesive layer 250 may include a hollowed-out region 251, the shape of the hollowed-out region 251 being the same or substantially the same as the orthographic projection of the opening 218 on the second adhesive layer 250.
For example, in other embodiments, when the second elastic film layer 240 is bonded to the substrate 210 by using a thermal compression method, an ultrasonic welding method, a photo adhesive bonding method, a chemical solvent bonding method, a laser welding method, or the like, the second adhesive layer 250 may be omitted. For example, when the second elastic film layer 240 and the substrate 210 are formed of the same polymer material, the second elastic film layer 240 and the substrate 210 may be bonded by laser welding without providing the second adhesive layer 250.
Fig. 12A and 12B are cross-sectional views taken along linebase:Sub>A-base:Sub>A' in fig. 9, and fig. 12A and 12B illustratebase:Sub>A liquid releasing process of the detection chip shown in fig. 9, according to at least one embodiment of the present disclosure. As shown in fig. 12A and 12B, the channels 214 may be broken when the push rod applies a force towards the bottom of the reservoir 212 (e.g., the central region 213), the bottom of the reservoir 212 is split along the channels 214, the edge of the central region 213 surrounded by the channels 214 is separated from the rest of the bottom of the reservoir 212, the connection 217 connects the central region 213 with the side walls 216, and the connection 217 allows the angle between the central region 213 and the side walls 216 to be an angle greater than zero degrees and less than 90 degrees, such as an angle of 10 degrees to 25 degrees. The liquid channel 215 is connected to the channel 214, and the liquid discharged from the liquid storage chamber 212 flows into the liquid channel 215 and is transported to other positions of the detection chip, such as the liquid operation area 219, through the liquid channel 215.
At least one embodiment of the present disclosure also provides a method for preparing a detection chip, which may be the detection chip provided in any of the above embodiments. For a detailed description of the detection chip, reference may be made to the description of the above embodiments, which will not be repeated herein.
For example, a method for preparing a detection chip according to at least one embodiment of the present disclosure may include:
providing a substrate, the substrate comprising a first surface,
wherein the substrate includes a reservoir configured to contain a liquid and including a bottom portion at the first surface,
the bottom portion includes a central region and an edge region surrounding the central region, an
The edge region includes a channel surrounding a central region, the channel having a thickness less than a thickness of the central region.
In some embodiments, the method of preparing a detection chip may further include: the substrate is prepared by an integral molding method. For example, the integrated molding method includes an injection molding process, and the substrate can be prepared through the injection molding process and by using a corresponding injection mold, so that the cost is saved and the production efficiency is improved. In some embodiments, when the substrate is prepared by an injection molding process, a liquid channel and the like may also be formed in the substrate at the same time, which is not limited by the embodiments of the present disclosure.
In some embodiments, the method of preparing a detection chip may further include: a first elastic film layer is provided on a first surface of a substrate. The first elastic film layer is hermetically connected with the first surface of the substrate, and at least covers the bottom of the liquid storage chamber. For the description of the first elastic film layer, reference may be made to the description of the above embodiments, which will not be repeated herein.
In some embodiments, the method of preparing a detection chip may further include: the first elastic film layer and the substrate are joined by laser welding or an adhesive. For example, when the first elastic film layer and the substrate are formed of the same material (e.g., polymer material such as PS, PMMA, PC, PP, etc.), the first elastic film layer and the substrate may be joined by laser welding; when the first elastic film layer and the substrate are formed of different materials, the first elastic film layer and the substrate may be joined by, for example, an adhesive.
In some embodiments, the step of joining the first elastic film layer and the substrate by an adhesive may comprise: a first adhesive layer is disposed between the first elastic film layer and the substrate. For the description of the first adhesive layer, reference may be made to the description of the above embodiments, which will not be repeated herein.
In some embodiments, the method of preparing a detection chip may further include: a second elastomeric film layer is provided on a second surface of the substrate opposite the first surface. The first elastic film layer is hermetically connected with the second surface of the substrate. The second elastic film layer covers, for example, at least an opening of the reservoir at the second surface. The description of the second elastic film layer can be referred to the description of the above embodiments, and will not be repeated herein.
In some embodiments, the method of preparing a detection chip may further include: the second elastic film layer and the substrate are joined by laser welding or an adhesive. For example, when the second elastic film layer and the substrate are formed of the same material (e.g., polymer material such as PS, PMMA, PC, PP, etc.), the second elastic film layer and the substrate may be joined by laser welding; when the second elastic film layer and the substrate are formed of different materials, the second elastic film layer and the substrate may be joined by, for example, an adhesive.
In some embodiments, the step of joining the second elastic film layer and the substrate by an adhesive may comprise: a second adhesive layer is disposed between the second elastic film layer and the substrate. For the description of the second adhesive layer, reference may be made to the description of the above embodiments, which will not be repeated herein.
At least one embodiment of the present disclosure also provides a method for operating a detection chip, wherein the detection chip may be the detection chip provided in any of the above embodiments. For the detailed description of the detection chip, reference may be made to the description of the above embodiments, which will not be repeated herein.
For example, a method for operating a detection chip according to at least one embodiment of the present disclosure may include:
a force is applied on the bottom of the reservoir toward the interior space of the reservoir to break the channel and cause the liquid contained within the interior space of the reservoir to flow out of the bottom of the reservoir.
In some embodiments, applying a force on the bottom of the reservoir toward the interior space of the reservoir to break the channel and cause the liquid contained within the interior space of the reservoir to flow out of the bottom of the reservoir includes: applying the force on the bottom of the detection chip toward the interior space of the reservoir such that the central region makes an angle with the sidewall that is greater than zero degrees and less than 90 degrees. For example, the central region makes an angle with the sidewall of 0.1 to 60 degrees, 5 to 40 degrees, 10 to 25 degrees.
In some embodiments, in the case that the detection chip further includes a first elastic film layer, the step of applying a force on the bottom of the reservoir toward the inner space of the reservoir may include: by applying an acting force on the first elastic film layer to apply an acting force to the bottom of the liquid storage chamber, the channel is broken and the liquid contained in the inner space of the liquid storage chamber is made to flow out from the bottom of the liquid storage chamber. For the description of the first elastic film layer, reference may be made to the description of the above embodiments, which will not be repeated herein.
In some embodiments, applying a force to the bottom of the reservoir by applying a force on the first resilient film layer to break the channel and allow the liquid contained within the interior space of the reservoir to flow out of the bottom of the reservoir may include: an application force is applied to the first resilient film layer at a location corresponding to a location spaced from the connection portion by a length of 0-3/4 of the central region to apply an application force to the bottom of the reservoir, thereby breaking the channel and allowing liquid contained within the interior of the reservoir to flow out of the bottom of the reservoir. For example, a force may be applied to the first elastic film layer at a location corresponding to a location 1/2 of the length of the central region from the attachment portion. Can make like this under the same elastic deformation condition of first elasticity rete production, central zone and lateral wall become littleer angle, and the liquid in the inner space of the stock solution room of being convenient for flows from the stock solution room bottom. The length of the central region as referred to herein refers to the distance from the coupling portion to a portion of the side wall of the reservoir on the first surface, wherein the portion of the side wall is on opposite sides of the central region from the coupling portion.
In some embodiments, in the case where the detection chip further includes a second elastic film layer, the method for operating the detection chip may further include: a force is applied to the second resilient film layer to urge the liquid contained within the interior space of the reservoir out of the bottom of the reservoir. For the description of the second elastic film layer, reference may be made to the description of the above embodiments, which will not be repeated herein.
Fig. 13 is a schematic block diagram of a detection apparatus in accordance with at least one embodiment of the present disclosure. As shown in fig. 13, a detection apparatus 300 according to at least one embodiment of the present disclosure may include:
a detection chip 310; and
a force application mechanism 320 configured to apply a force on the bottom of the detection chip 310 toward the inner space of the liquid reservoir to break the channel and allow the liquid contained in the inner space to flow out from the bottom of the detection chip 310 in use.
The detection chip 310 may be the detection chip provided in any of the above embodiments. The force application mechanism 320 can take any suitable form so long as it can apply a force to the bottom of the detection chip 310 to break the channel at the bottom of the reservoir in the detection chip 310. For example, the force applying mechanism 320 may include a rod to apply pressure to the bottom of the reservoir to form an opening in the bottom of the reservoir to allow the liquid stored therein to flow out, and an end of the rod may be engaged with an end surface of the bottom of the reservoir. The force application mechanism 320 may be motor driven or may be manually operated, as embodiments of the present disclosure are not limited in this respect.
In some embodiments, where the substrate of the detection chip 310 includes a connection, after the channel at the bottom of the reservoir is broken and the central region is at an angle greater than zero degrees and less than 90 degrees (e.g., an angle of 10-25 degrees) with the sidewalls, the force application mechanism 320 can be withdrawn or held against the bottom of the detection chip 310 such that the central region remains at the formed angle with the sidewalls. For example, the force application mechanism 320 may be removed in the event that the bending of the connection is plastically deformed when the channel at the bottom of the reservoir is broken and the central region is at an angle greater than zero degrees and less than 90 degrees (e.g., an angle of 10-25 degrees) with the sidewall. For example, in the case where the channel of the bottom of the reservoir is broken and the central region and the sidewall form an angle greater than zero degrees and less than 90 degrees (e.g., an angle of 10 degrees to 25 degrees), the force applying mechanism 320 may be held against the bottom of the detection chip 310 under the condition that the bending of the connecting portion is elastic deformation, so that the central region and the sidewall maintain the formed angle, thereby preventing the central region from becoming horizontally placed again with the flow of the liquid in the reservoir and blocking the opening of the bottom of the reservoir.
Although not shown in fig. 13, it is understood that the detection apparatus 300 may further include a base for placing the detection chip 310, a waste liquid processor, various analysis detectors, a liquid input/output interface, a power interface, and the like, which may all adopt components known in the art, and the embodiment of the present disclosure is not limited thereto.
The above description is intended to be merely exemplary embodiments of the present disclosure and is not intended to limit the scope of the present disclosure, which is defined by the claims appended hereto.
Claims (21)
- A detection chip, comprising:a substrate comprising a first surface;wherein the substrate includes a reservoir configured to contain a liquid and including a bottom on the first surface,the bottom portion includes a central region and an edge region surrounding the central region,the edge regions comprise a channel surrounding the central region, the channel having a thickness less than a thickness of the central region.
- The detection chip of claim 1, wherein the channel is a non-enclosed channel to partially surround the central region.
- The detection chip of claim 1, wherein the channel is a closed channel to surround the central region by one circle.
- The detection chip according to claim 1 or 2, wherein the reservoir has a cylindrical shape and the channel has an annular shape.
- The detection chip of claim 4, wherein the central angle of the channel is 340-358 degrees.
- The detection chip of any one of claims 1 to 5, wherein the thickness of the central region is 0.1mm to 3mm, andthe thickness of the channel is 0.05mm-0.3mm, and the width of the channel is 0.1mm-3mm.
- The detection chip according to any one of claims 1 to 6, wherein a surface of the bottom facing the interior space of the reservoir is planar, and the channel is recessed at the first surface facing the interior space of the reservoir.
- The detection chip according to any one of claims 1 to 6, wherein a face of the bottom portion facing away from the interior space of the reservoir is planar, and the channels are recessed away from the interior space of the reservoir at the face of the bottom portion facing toward the interior space of the reservoir.
- The detection chip of claim 1 or 2,the reservoir further comprising a sidewall, the channel being between the sidewall and the central region,the edge region further comprises a connecting portion which,wherein the connection is between the sidewall and the central region, and the connection corresponds to an opening portion of the channel that does not surround the central region.
- The detection chip of claim 9, wherein the connection is configured to allow the central region to make an angle with the sidewall greater than zero degrees and less than 25 degrees in the event the channel is disrupted.
- The detection chip of claim 9 or 10, wherein the substrate further comprises a liquid flow channel on the first surface,wherein the liquid flow channel is communicated with the channel.
- The detection chip of claim 11, wherein the connection portion and the liquid flow path are located on opposite sides of the central region on the first surface.
- The detection chip of claim 11 or 12, further comprising: a first elastic film layer, a second elastic film layer,wherein the liquid flow channel includes a groove on the first surface of the substrate,the first elastic film layer is arranged on the first surface of the substrate, covers the bottom and covers the groove to provide a liquid flowing space.
- The detection chip of claim 13, further comprising a second elastic film layer,the substrate further comprises a second surface opposite the first surface,the second elastic film layer is arranged on the second surface of the substrate and covers the opening of the liquid storage chamber on the second surface.
- The detection chip of claim 14, further comprising: a first adhesive layer and a second adhesive layer,wherein the first adhesive layer is between the substrate and the first elastic film layer to connect the substrate and the first elastic film layer; andthe second adhesive layer is between the substrate and the second elastic film layer to connect the substrate and the second elastic film layer.
- The detection chip of any one of claims 11 to 15, further comprising a liquid handling zone,the liquid operation area is communicated with the liquid flow passage,wherein the liquid handling zone comprises at least one selected from the group consisting of a liquid mixing zone, a liquid detection zone, and a liquid storage zone.
- A detection device, comprising:the detection chip according to any one of claims 1 to 16; anda force application mechanism configured to, in use, apply a force on the bottom portion of the detection chip towards the interior space of the reservoir to break the channel and cause liquid contained within the interior space to flow out of the bottom portion.
- A method for preparing a detection chip, comprising:providing a substrate, the substrate comprising a first surface,wherein the substrate includes a reservoir configured to contain a liquid and including a bottom portion at the first surface,the bottom portion includes a central region and an edge region surrounding the central region, anThe edge regions comprise a channel surrounding the central region, the channel having a thickness less than a thickness of the central region.
- The method as recited in claim 18, further comprising: the substrate is prepared by an integral molding method.
- A method for operating the detection chip of any one of claims 1 to 16, comprising:applying a force on the bottom of the reservoir toward an interior space of the reservoir to break the channel and allow liquid contained within the interior space to flow out of the bottom.
- A method for operating the detection chip of claim 13, comprising:applying a force to the bottom of the reservoir at a location on the first resilient film layer corresponding to a location 0-3/4 of the length of the central region from the connection to break the channel and allow liquid contained within the interior space of the reservoir to flow out of the bottom.
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| PCT/CN2020/081082 WO2021189290A1 (en) | 2020-03-25 | 2020-03-25 | Detection chip, detection device, and methods for manufacturing and operating detection chip |
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| US20160220994A1 (en) * | 2015-01-29 | 2016-08-04 | David W. Wright | Diagnostic cartridge, fluid storage and delivery apparatus therefor and methods of construction thereof |
| CN206965753U (en) * | 2017-06-09 | 2018-02-06 | 北京百康芯生物科技有限公司 | A kind of micro-fluidic chip with reagent memory cell |
| CN109557150A (en) * | 2019-01-14 | 2019-04-02 | 大连大学 | Digital microcurrent-controlled chip and pathogen immunologic detection method based on it |
| CN110632286A (en) * | 2019-10-28 | 2019-12-31 | 南京晶捷生物科技有限公司 | Biochemical detection equipment |
| CN114100702A (en) * | 2020-08-27 | 2022-03-01 | 京东方科技集团股份有限公司 | Detection chip, preparation method, use method and detection device thereof |
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| WO2021189290A1 (en) | 2021-09-30 |
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