CN115558577B - A fermentation desublimation crystallization device for tetramethylpyrazine - Google Patents

A fermentation desublimation crystallization device for tetramethylpyrazine

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Publication number
CN115558577B
CN115558577B CN202211254577.6A CN202211254577A CN115558577B CN 115558577 B CN115558577 B CN 115558577B CN 202211254577 A CN202211254577 A CN 202211254577A CN 115558577 B CN115558577 B CN 115558577B
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fermentation tank
fermentation
spiral coil
tetramethylpyrazine
water inlet
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CN115558577A (en
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孟武
石林
林�源
秦伟帅
王腾飞
邵丽颖
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Qilu University of Technology
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Qilu University of Technology
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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Abstract

The invention provides a fermentation desublimation crystallization device of tetramethylpyrazine, which comprises a fermentation tank, a spiral coil, a leaching spray head and a stirring mechanism, wherein the spiral coil is positioned at the upper position inside the fermentation tank, the leaching spray head is positioned above the spiral coil, the stirring end of the stirring mechanism is positioned at the lower position inside the fermentation tank, a PH electrode, a temperature electrode and an oxygen dissolving electrode for monitoring the state of fermentation broth are arranged on the side wall of the fermentation tank, a discharge pipe is arranged at the bottom end of the fermentation tank, and an inoculation port, an air inlet and an air outlet are arranged at the top end of the fermentation tank.

Description

Fermentation desublimation crystallization device of tetramethylpyrazine
Technical Field
The invention relates to the field of bioengineering, in particular to a fermentation desublimation crystallization device of tetramethylpyrazine.
Background
Tetramethyl pyrazine is a common heterocyclic nitrogen compound, is widely applied to food addition and treatment of cardiovascular and cerebrovascular diseases, has higher and higher attention, and is a better production method for tetramethyl pyrazine, and the production method of tetramethyl pyrazine is continuously improved and optimized, but still has certain problems. Fermentation purification is a key link for producing tetramethylpyrazine, and the link affects the quality and the acquisition amount of tetramethylpyrazine to a great extent. At present, tetramethylpyrazine fermentation liquor is usually placed in a fermentation tank, tetramethylpyrazine is extracted from the fermentation liquor by a biological fermentation method, the concentration of tetramethylpyrazine in the fermentation liquor by the biological fermentation method is low, more tetramethylpyrazine products cannot be obtained in the subsequent purification process, the purification process is complex, and the purification cost is high. Meanwhile, the solubility of the tetramethylpyrazine in the fermentation liquor is very small, and crystals are generated in the fermentation process and attached to the inner wall of the fermentation tank after the fermentation liquor is saturated, so that the tetramethylpyrazine obtained by purifying the fermentation liquor is very little, and therefore, a device which can improve the purification efficiency and purification amount of the tetramethylpyrazine, has simple purification steps and is low in cost is needed.
Disclosure of Invention
In order to solve the problems in the background technology, the invention provides a fermentation desublimation crystallization device of tetramethylpyrazine, which comprises a fermentation tank, a spiral coil pipe, a leaching spray head and a stirring mechanism, wherein the spiral coil pipe is positioned at the upper position inside the fermentation tank, the leaching spray head is positioned above the spiral coil pipe, the stirring end of the stirring mechanism is positioned at the lower position inside the fermentation tank, a PH electrode, a temperature electrode and an oxygen dissolving electrode for monitoring the state of fermentation broth are arranged on the side wall of the fermentation tank, a discharge pipe is arranged at the bottom end of the fermentation tank, and an inoculation port, an air inlet and an air outlet are arranged at the top end of the fermentation tank.
Preferably, the spiral coil comprises a spiral coil water inlet and a spiral coil water outlet, the spiral coil water inlet and the spiral coil water outlet extend out of the fermentation tank, and the spiral coil water inlet and the spiral coil water outlet are positioned at the top end of the fermentation tank.
Preferably, an air inlet pipe is arranged in the fermentation tank, one end of the air inlet pipe is communicated with the air inlet, the other end of the air inlet pipe extends to a position, close to the bottom, in the fermentation tank, and a plurality of air holes are formed in one end, close to the bottom, of the fermentation tank, of the air inlet pipe.
Preferably, the stirring mechanism comprises a motor, a shaft rod and an anchor frame stirrer, wherein the motor is positioned at the top end outside the fermentation tank, the shaft rod is positioned inside the fermentation tank, one end of the shaft rod extends out of the fermentation tank and is connected with the output end of the motor, the other end of the shaft rod is connected with the anchor frame stirrer, and the anchor frame stirrer is positioned at the position below the inside of the fermentation tank.
Preferably, a jacket is arranged below the side wall of the fermentation tank, and a jacket water outlet and a jacket water inlet are arranged on the jacket.
Preferably, a sight glass is arranged above the side wall of the fermentation tank.
Preferably, the top end of the fermentation tank is provided with a defoaming agent port, an acid-base agent port, a material supplementing port and a water inlet of the leaching spray head, and the water inlet end of the leaching spray head is connected with the water inlet of the leaching spray head.
Preferably, a discharge valve is arranged on the discharge pipe.
Preferably, a bracket for supporting the fermentation tank is connected below the fermentation tank.
The device has the advantages that the device can control the temperature, the surface area of the interior of the fermentation tank is increased by the spiral coil, after water with a certain temperature is introduced into the spiral coil, the tetramethylpyrazine in the fermentation liquid volatilizes and desublimates, a large amount of crystals are attached to the spiral coil, the crystallization amount and the crystallization rate of the tetramethylpyrazine are improved, and the crystals are sprayed and dissolved by the leaching spray head, so that the crystals are convenient to collect. The device of the invention increases the purification efficiency of tetramethylpyrazine, effectively reduces the impurity content of the purified product, and saves the steps and the cost of fermentation liquor purification.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
fig. 2 is a top view of the present invention.
The reference numbers in the figure are 1-bracket, 2-discharge valve, 3-discharge pipe, 4-jacket water inlet, 5-jacket, 6-pH electrode, 7-temperature electrode, 8-dissolved oxygen electrode, 9-sight glass, 10-motor, 11-eluting nozzle, 12-fermentation tank, 13-crystallizing coil, 14-air inlet pipe, 15-shaft lever, 16-jacket water outlet, 17-anchor frame stirrer, 18-feeding port, 19-defoamer port, 20-acid-alkali agent port, 21-crystallizing coil water inlet, 22-inoculation port, 23-eluting nozzle water inlet, 24-air outlet, 25-air inlet and 26-crystallizing coil water outlet.
Detailed Description
In order to make the technical solutions, objects and advantages of the present invention more apparent, the present invention will be further described with reference to the accompanying drawings and examples, it being understood that the examples provided are only one way of implementing the technical solutions and do not represent all the examples, and any non-inventive examples made by those skilled in the art on the basis of the present examples are within the scope of the present invention.
Referring to fig. 1 and 2, a device for fermenting and desublimating and crystallizing tetramethylpyrazine comprises a fermentation tank 12, a spiral coil 13, a leaching spray nozzle 11 and a stirring mechanism, wherein the spiral coil 13 is positioned at the upper position inside the fermentation tank 12, the leaching spray nozzle 11 is positioned above the spiral coil 13, the stirring end of the stirring mechanism is positioned at the lower position inside the fermentation tank 12, a PH electrode 6, a temperature electrode 7 and an oxygen dissolving electrode 8 for monitoring the state of fermentation broth are arranged on the side wall of the fermentation tank 12, the PH electrode 6 is used for monitoring the PH value of fermentation broth, the temperature electrode 7 is used for monitoring the temperature of fermentation broth, the oxygen dissolving electrode 8 is used for monitoring the oxygen dissolving amount of fermentation broth, a discharge pipe 3 is arranged at the bottom end of the fermentation tank 12, and an inoculation port 22, an air inlet 25 and an air outlet 24 are arranged at the top end of the fermentation tank 12.
Specifically, the spiral coil 13 includes a spiral coil water inlet 21 and a spiral coil water outlet 26, the spiral coil water inlet 21 and the spiral coil water outlet 26 extend out of the fermentation tank 12, and the spiral coil water inlet 21 and the spiral coil water outlet 26 are located at the top end of the fermentation tank 12.
Specifically, an air inlet pipe 14 is arranged in the fermentation tank 12, one end of the air inlet pipe 14 is communicated with an air inlet 25, the air inlet pipe 14 is connected with an air compressor through the air inlet 25, the air compressor is responsible for introducing air into the fermentation tank 12, the other end of the air inlet pipe 14 extends to a position close to the bottom in the fermentation tank 12, and a plurality of air holes are formed in one end, close to the bottom of the fermentation tank 12, of the air inlet pipe 14.
Specifically, the stirring mechanism comprises a motor 10, a shaft lever 15 and an anchor frame stirrer 17, wherein the motor 10 is positioned at the top end outside the fermentation tank 12, the shaft lever 15 is positioned inside the fermentation tank 12, one end of the shaft lever 15 extends out of the fermentation tank 12 and is connected with the output end of the motor 10, the other end of the shaft lever 15 is connected with the anchor frame stirrer 17, and the anchor frame stirrer 17 is positioned at the position below the inside of the fermentation tank 12.
Specifically, a jacket 5 is arranged below the side wall of the fermentation tank 12, a jacket water outlet 16 and a jacket water inlet 4 are arranged on the jacket 5, and the jacket 5 is responsible for heating and cooling the fermentation tank 12 and maintaining the temperature environment of fermentation.
Specifically, a sight glass 9 is arranged above the side wall of the fermentation tank 12, and the crystallization condition and the crystallization dissolution condition in the fermentation tank 12 are observed through the sight glass 9.
Specifically, the top end of the fermentation tank 12 is provided with a defoamer port 19, an acid-base agent port 20, a feed supplement port 18 and a leaching spray nozzle water inlet 23, the water inlet end of the leaching spray nozzle 11 is connected with the leaching spray nozzle water inlet 23, the defoamer is supplemented in the fermentation process through the defoamer port 19, the acid liquor or alkali liquor is supplemented in the fermentation process through the acid-base agent port 20, and the substrate is supplemented in the fermentation process through the feed supplement port 18.
Specifically, the discharge pipe 3 is provided with a discharge valve 2.
Specifically, a bracket 1 for supporting the fermentation tank 12 is connected below the fermentation tank 12.
The working flow is that fermentation seed liquid is inoculated into a fermentation tank 12 containing sterile fermentation liquid through an inoculation port 22, a PH electrode 6, a temperature electrode 7 and an oxygen dissolving electrode 8 are started, a motor 10 is started, the motor 10 drives an anchor frame type stirrer 17 to rotate through a shaft lever 15, fermentation liquid is stirred to promote fermentation, tetramethylpyrazine in the fermentation liquid is easy to reach saturation in the fermentation process, the tetramethylpyrazine volatilizes to the upper part of the fermentation tank 12 after saturation, water at 15-20 ℃ is introduced into a crystallization coil 13 through a crystallization coil water inlet 21, and a large amount of crystals are adhered to the spiral coil 13, so that the temperature of the upper part of the fermentation tank 12 can lead the volatilized tetramethylpyrazine to be desublimated. After fermentation, the discharging valve 2 is opened, fermentation liquid is discharged through the discharging pipe 3, the discharging valve 2 is closed after the fermentation liquid is discharged, hot water is injected into the water inlet 23 of the leaching spray nozzle, the hot water is sprayed and dissolved through the leaching spray nozzle 11 at the top of the fermentation tank 12, the dissolved crystals flow to the bottom of the fermentation tank 12, and the discharging valve 2 is opened again to collect the dissolved crystals, so that the next purification and recovery of tetramethylpyrazine are facilitated.
The above embodiments merely illustrate the basic principles and features of the present invention, but are not limited by the above embodiments, it should be understood that various changes and modifications can be made by those skilled in the art without departing from the spirit and scope of the invention, and these changes and modifications fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (5)

1.一种四甲基吡嗪的发酵凝华结晶装置，其特征在于：包括发酵罐（12）、螺旋盘管（13）、淋洗喷头（11）和搅拌机构，所述螺旋盘管（13）位于发酵罐（12）内部上方位置，淋洗喷头（11）位于发酵罐（12）内部，且淋洗喷头（11）位于螺旋盘管（13）上方，搅拌机构搅拌端位于发酵罐（12）内部下方位置，所述发酵罐（12）侧壁上设有用于监测发酵液状态的PH电极（6）、温度电极（7）和溶氧电极（8），发酵罐（12）底端设有排料管（3），发酵罐（12）顶端设有接种口（22）、进气口（25）和出气口（24）；1. A fermentation-desublimation crystallization device for tetramethylpyrazine, characterized in that it comprises a fermentation tank (12), a spiral coil (13), a rinsing nozzle (11) and a stirring mechanism, wherein the spiral coil (13) is located at an upper position inside the fermentation tank (12), the rinsing nozzle (11) is located inside the fermentation tank (12), and the rinsing nozzle (11) is located above the spiral coil (13), and the stirring end of the stirring mechanism is located at a lower position inside the fermentation tank (12), a pH electrode (6), a temperature electrode (7) and a dissolved oxygen electrode (8) for monitoring the state of the fermentation liquid are provided on the side wall of the fermentation tank (12), a discharge pipe (3) is provided at the bottom end of the fermentation tank (12), and an inoculation port (22), an air inlet (25) and an air outlet (24) are provided at the top end of the fermentation tank (12); 所述螺旋盘管（13）包括螺旋盘管进水口（21）和螺旋盘管出水口（26），螺旋盘管进水口（21）和螺旋盘管出水口（26）延伸至发酵罐（12）外，螺旋盘管进水口（21）和螺旋盘管出水口（26）位于发酵罐（12）顶端；The spiral coil (13) includes a spiral coil water inlet (21) and a spiral coil water outlet (26), the spiral coil water inlet (21) and the spiral coil water outlet (26) extending outside the fermentation tank (12), and the spiral coil water inlet (21) and the spiral coil water outlet (26) are located at the top of the fermentation tank (12); 所述发酵罐（12）内设有进气管（14），进气管（14）一端与进气口（25）连通，进气管（14）另一端延伸至发酵罐（12）内靠近底部位置，进气管（14）靠近发酵罐（12）底部的一端设有多个气孔；An air inlet pipe (14) is provided in the fermentation tank (12), one end of the air inlet pipe (14) is connected to the air inlet (25), and the other end of the air inlet pipe (14) extends to a position near the bottom of the fermentation tank (12), and a plurality of air holes are provided at one end of the air inlet pipe (14) near the bottom of the fermentation tank (12); 所述搅拌机构包括电机（10）、轴杆（15）和锚框式搅拌器（17），电机（10）位于发酵罐（12）外部顶端，轴杆（15）位于发酵罐（12）内部，轴杆（15）一端延伸至发酵罐（12）外与电机（10）输出端连接，轴杆（15）另一端连接锚框式搅拌器（17），锚框式搅拌器（17）位于发酵罐（12）内部下方位置；The stirring mechanism comprises a motor (10), a shaft (15) and an anchor frame stirrer (17), wherein the motor (10) is located at the top end of the outside of the fermentation tank (12), the shaft (15) is located inside the fermentation tank (12), one end of the shaft (15) extends outside the fermentation tank (12) and is connected to the output end of the motor (10), and the other end of the shaft (15) is connected to the anchor frame stirrer (17), and the anchor frame stirrer (17) is located at a lower position inside the fermentation tank (12); 所述发酵罐（12）顶端设有消泡剂口（19）、酸碱剂口（20）、补料口（18）以及淋洗喷头进水口（23），淋洗喷头（11）进水端与淋洗喷头进水口（23）连接。The top of the fermentation tank (12) is provided with a defoaming agent port (19), an acid and alkali agent port (20), a feed port (18) and a rinsing nozzle water inlet (23), and the water inlet end of the rinsing nozzle (11) is connected to the rinsing nozzle water inlet (23). 2.根据权利要求1所述的一种四甲基吡嗪的发酵凝华结晶装置，其特征在于：所述发酵罐（12）侧壁下方位置设有夹套（5），夹套（5）上设有夹套出水口（16）和夹套进水口（4）。2. A fermentation desublimation crystallization device for tetramethylpyrazine according to claim 1, characterized in that a jacket (5) is provided below the side wall of the fermentation tank (12), and the jacket (5) is provided with a jacket water outlet (16) and a jacket water inlet (4). 3.根据权利要求1所述的一种四甲基吡嗪的发酵凝华结晶装置，其特征在于：所述发酵罐（12）侧壁上方位置设有视镜（9）。3. The fermentation desublimation crystallization device of tetramethylpyrazine according to claim 1, characterized in that a sight glass (9) is provided above the side wall of the fermentation tank (12). 4.根据权利要求1所述的一种四甲基吡嗪的发酵凝华结晶装置，其特征在于：所述排料管（3）上设有排料阀（2）。4. The fermentation desublimation crystallization device of tetramethylpyrazine according to claim 1, characterized in that a discharge valve (2) is provided on the discharge pipe (3). 5.根据权利要求1所述的一种四甲基吡嗪的发酵凝华结晶方法，其特征在于：所述发酵罐（12）下方连接有用于支撑发酵罐（12）的支架（1）。5. The method for fermentation desublimation crystallization of tetramethylpyrazine according to claim 1, characterized in that a support (1) for supporting the fermentation tank (12) is connected below the fermentation tank (12).
CN202211254577.6A 2022-10-13 2022-10-13 A fermentation desublimation crystallization device for tetramethylpyrazine Active CN115558577B (en)

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