CN115530155A - 24-hour urine sample preservative, preparation method and application - Google Patents
24-hour urine sample preservative, preparation method and application Download PDFInfo
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- CN115530155A CN115530155A CN202110729525.9A CN202110729525A CN115530155A CN 115530155 A CN115530155 A CN 115530155A CN 202110729525 A CN202110729525 A CN 202110729525A CN 115530155 A CN115530155 A CN 115530155A
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- isothiazolin
- urine sample
- methyl
- hour urine
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- 210000002700 urine Anatomy 0.000 title claims abstract description 124
- 239000003755 preservative agent Substances 0.000 title claims abstract description 86
- 230000002335 preservative effect Effects 0.000 title claims abstract description 83
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims abstract description 36
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000001514 detection method Methods 0.000 claims abstract description 31
- 229940100484 5-chloro-2-methyl-4-isothiazolin-3-one Drugs 0.000 claims abstract description 24
- DHNRXBZYEKSXIM-UHFFFAOYSA-N chloromethylisothiazolinone Chemical compound CN1SC(Cl)=CC1=O DHNRXBZYEKSXIM-UHFFFAOYSA-N 0.000 claims abstract description 24
- 229910001629 magnesium chloride Inorganic materials 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910001868 water Inorganic materials 0.000 claims abstract description 7
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims description 36
- BEGLCMHJXHIJLR-UHFFFAOYSA-N methylisothiazolinone Chemical compound CN1SC=CC1=O BEGLCMHJXHIJLR-UHFFFAOYSA-N 0.000 claims description 26
- 229940100555 2-methyl-4-isothiazolin-3-one Drugs 0.000 claims description 23
- UEFCKYIRXORTFI-UHFFFAOYSA-N 1,2-thiazolidin-3-one Chemical compound O=C1CCSN1 UEFCKYIRXORTFI-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 6
- 239000003381 stabilizer Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 abstract description 9
- 241000894006 Bacteria Species 0.000 abstract description 8
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 abstract description 6
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 abstract description 6
- 229940116269 uric acid Drugs 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 150000002500 ions Chemical class 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 2
- 238000005536 corrosion prevention Methods 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 81
- 238000012360 testing method Methods 0.000 description 6
- 239000013078 crystal Chemical class 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 241000194017 Streptococcus Species 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 241000588769 Proteus <enterobacteria> Species 0.000 description 3
- 241000191940 Staphylococcus Species 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical class OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 2
- 239000012496 blank sample Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 159000000000 sodium salts Chemical class 0.000 description 2
- -1 specific gravity Substances 0.000 description 2
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- OBHRVMZSZIDDEK-UHFFFAOYSA-N urobilinogen Chemical class CCC1=C(C)C(=O)NC1CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(CC3C(=C(CC)C(=O)N3)C)N2)CCC(O)=O)N1 OBHRVMZSZIDDEK-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 239000005844 Thymol Substances 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 238000009535 clinical urine test Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 208000017169 kidney disease Diseases 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229960000790 thymol Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a 24-hour urine sample preservative, a preparation method and application. The 24-hour urine sample preservative comprises the components of 5-chloro-2-methyl-4-isothiazolin-3-one, magnesium chloride, magnesium nitrate and water. The preparation method of the 24-hour urine sample preservative comprises the steps of proportioning and mixing corresponding raw materials to prepare liquid for urine detection. The urine sample preservative has obvious effects on corrosion prevention and sterilization and strong bacteriostatic ability, can keep the content of substances in 24 urine samples while inhibiting the growth of bacteria in the urine for 24 hours, does not interfere with the detection result, does not react with detection indexes such as protein and uric acid, and does not influence the detection of various ions, thereby ensuring the accuracy of clinical urine detection results.
Description
Technical Field
The invention relates to the technical field of chemical preparations used for medical detection, in particular to a chemical sample preservative for 24-hour urine detection, a preparation method and application.
Background
The 24-hour urine is a clinically very important examination method, particularly, the 24-hour quantitative detection of urine protein is considered as a clinical gold standard for urine protein detection, and the detection has important significance for diagnosis, treatment and evaluation of treatment effects of kidney diseases, but the 24-hour urine specimen retention process is relatively long, substances in the long 24-hour urine specimen can be decomposed, deteriorated, polluted and the like, and in order to stabilize substances in urine, a sample preservative needs to be added into the 24-hour urine specimen to ensure the quality of the specimen, so that accurate reference data is provided for final disease diagnosis.
Currently, most urine sample preservatives used in clinical practice are classified into the following categories: concentrated hydrochloric acid, methylbenzene, dimethylbenzene, glacial acetic acid, thymol and formaldehyde, but most of the sample treating agents belong to volatile, flammable and explosive chemical dangerous goods, cause great damage to human bodies after long-term contact, and can be safely used only by making professional protection. The above sample preservatives have some effects on urine preservation, but are not commonly used because different sample preservatives are required for different testing items, which are not favorable for clinical processing operation and selection. Therefore, the preservation of most clinical 24 hour urine samples is not standardized, which indirectly results in difficulties and delays in the development of 24 hour urine tests.
Therefore, the invention provides a 24-hour urine sample preservative, a preparation method and application, and effectively overcomes the defects of poor safety, flammability, explosiveness, volatility, great pollution to human bodies and environment and non-universality of reagents.
Disclosure of Invention
In order to solve one or more problems of a urine sample preservative in the background art, the invention provides a chemical sample preservative for 24-hour urine detection, a preparation method and application.
The invention provides a 24-hour urine sample preservative, which is prepared from 1.5-4% of isothiazoline-3-ketone mixture; wherein the isothiazolin-3-one mixture comprises 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, and the molar ratio of the 5-chloro-2-methyl-4-isothiazolin-3-one to the 2-methyl-4-isothiazolin-3-one is 2;
further, the 24-hour urine sample preservative has the isothiazoline-3-ketone mixture accounting for 2% -3%; preferably 2.5%;
further, in the 24-hour urine sample preservative, the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the isothiazolin-3-one mixture is (2.8); preferably 3;
further, the 24-hour urine sample preservative comprises the following components in corresponding important proportion: the mixture of 5-chloro-2-methyl-4-isothiazoline-3-ketone and 2-methyl-4-isothiazoline-3-ketone accounts for 1.5-4%, magnesium chloride accounts for 0.5-1.2%, and magnesium nitrate accounts for 20.0-22.0%;
further, the sample preservative comprises the following components in percentage by weight: the mixture of 5-chloro-2-methyl-4-isothiazoline-3-ketone and 2-methyl-4-isothiazoline-3-ketone accounts for 2% -3%, magnesium chloride is 0.5% -1.0%, and magnesium nitrate is 21.0% -22.0%.
In a preferred embodiment of the invention, the 24-hour urine sample preservative has the content ratio of the isothiazolin-3-one mixture of 2.5%;
in another preferred embodiment of the invention, the 24-hour urine sample preservative comprises 2% of isothiazolin-3-one mixture;
in another preferred embodiment of the present invention, in the 24-hour urine sample preservative, the isothiazolin-3-one mixture is 3%;
in a preferred embodiment of the present invention, in the 24-hour urine sample preservative, the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the isothiazolin-3-one mixture is 3;
in another preferred embodiment of the present invention, in the 24-hour urine sample preservative, the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the isothiazolin-3-one mixture is 2.8;
in another preferred embodiment of the present invention, in the 24-hour urine sample preservative, the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the isothiazolin-3-one mixture is 3.2;
in a preferred embodiment of the invention, the 24-hour urine sample preservative has a magnesium chloride content of 0.8%;
in another preferred embodiment of the present invention, in the 24-hour urine sample preservative, the content of the magnesium chloride is 1%;
in another preferred embodiment of the present invention, in the 24-hour urine sample preservative, the content of magnesium chloride is 0.5%;
in a preferred embodiment of the present invention, in the 24-hour urine sample preservative, the content of the magnesium nitrate is 21%;
in another preferred embodiment of the present invention, in the 24-hour urine sample preservative, the content of the magnesium nitrate is 22%;
in a preferred embodiment of the invention, the 24-hour urine sample preservative comprises a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one in an amount of 2.5%, magnesium chloride in an amount of 0.8%, and magnesium nitrate in an amount of 21%;
in another preferred embodiment of the present invention, the 24-hour urine sample preservative comprises 3% of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 1% of magnesium chloride and 22% of magnesium nitrate;
in another preferred embodiment of the present invention, the 24-hour urine sample preservative comprises a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one in an amount of 2%, magnesium chloride in an amount of 0.5%, and magnesium nitrate in an amount of 22%;
compared with the prior art, the invention has the beneficial effects that: compared with the traditional urine sample preservative, the 24-hour urine sample preservative can effectively inhibit the breeding of bacteria in urine, ensure that sample substances cannot be decomposed, deteriorated, polluted and the like in the 24-hour long urine collection process, and maintain the stability of detected substances in samples, thereby ensuring the accuracy of detection results.
The invention provides a preparation method of a 24-hour urine sample preservative, which comprises the following specific preparation steps:
s1, preparing corresponding raw materials according to the following weight proportion: 1.5-4% of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 0.5-1.2% of magnesium chloride and 20.0-22.0% of magnesium nitrate;
and S2, adding a proper amount of water into the components in the S1 at normal temperature, and uniformly mixing to obtain the 24-hour urine sample preservative.
Further, in the step S2, the percentage content of the added water is 74% -78%;
compared with the prior art, its beneficial effect lies in: the 24-hour urine sample preservative has the advantages of simple preparation method, no need of violent reaction conditions, extremely low toxicity, safety and stability, low preparation cost and capability of realizing large-scale production.
The invention also provides the use of a 24-hour urine sample preservative as defined in any one of the first aspect of the invention in the preparation of a container for urine testing and collection;
the invention also provides a 24-hour urine sample preservative in the first aspect of the invention, which is used for preserving and resisting bacteria of relevant test samples such as urine;
the invention also provides a 24-hour urine collection sample antibacterial method, which comprises the steps of applying an effective amount of the 24-hour urine sample preservative in the first aspect of the invention to a urine collection container;
in the application, the isothiazolin-3-ketone mixture is used as a main bacteriostatic component of the sample preservative; the magnesium chloride and the magnesium nitrate are used as stabilizers of the sample preservative;
compared with the prior art, its beneficial effect lies in: as a sample preservative used in urine detection, the liquid reagent can be suitable for urine detection containers in different scenes, and a patient can inject the sample preservative into a barrel before urine collection, so that the sample is prevented from being changed in quality after the urine collection is finished. The 24-hour urine sample preservative has the advantages of small usage amount, difficult exertion, safety and stability, no reaction with urine protein and uric acid to interfere the detection result, no influence on the detection of various ions, and capability of being used for detecting various components in 24-hour urine.
Detailed Description
In order to make the principle of the technical solution of the present invention clear and complete, the following lists contents of some embodiments and further elaborates the technical solution to be applied by the present invention.
Example 1, preparation of 24-hour urine sample preservative
The preparation raw materials comprise:
2.5 g of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the mixture is 3;
the preparation method specifically comprises the following steps:
weighing 2.5 g of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 0.8 g of magnesium chloride and 21 g of magnesium nitrate according to the weight of the preparation raw materials, uniformly mixing, adding 75.7 g of water, and uniformly stirring to obtain a 24-hour urine sample preservative;
example 2 preparation of 24-hour urine sample preservative
The preparation raw materials comprise:
3 g of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the mixture is 2.8;
the preparation method specifically comprises the following steps:
weighing 3 g of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 1 g of magnesium chloride and 22 g of magnesium nitrate according to the weight of the preparation raw materials, uniformly mixing, adding 74 g of water, and uniformly stirring to obtain a 24-hour urine sample preservative;
example 3 preparation of 24-hour urine sample preservative
The preparation raw materials comprise:
2 g of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the mixture is 3.2;
the preparation method specifically comprises the following steps:
weighing 2 g of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 0.5 g of magnesium chloride and 22 g of magnesium nitrate according to the weight of the preparation raw materials, uniformly mixing, adding 75.5 g of water, and uniformly stirring to obtain a 24-hour urine sample preservative;
test example 4, 24-hour antiseptic and antibacterial test of urine specimen preservative
Respectively taking 2 mL of the 24-hour urine sample preservative prepared in the embodiments 1-3, and respectively adding the 24-hour urine sample preservative into the 24-hour urine collecting containers A, B and C; taking the 24-hour urine collection container K without adding the 24-hour urine sample preservative prepared in the example 1 as a blank sample control; then collecting urine of four persons in 24-hour time periods from the urine collecting containers A, B, C and K respectively; sampling and detecting the collected urine;
the detection method comprises the following steps:
1 mL of urine sample to be detected is taken from urine in the urine collecting containers A, B, C and K respectively, 30mL of culture medium is added and mixed uniformly, then the mixture is transferred to a culture dish, the mixture is cultured for 48 hours +/-2 hours at 36 +/-1 ℃, and then the growth condition of the bacteria is checked;
the detection results are shown in Table 1;
TABLE 1
| Staphylococcus aureus | Escherichia coli | Streptococcus sp | Proteobacteria | |
| K | Growth with bacteria (colony count greater than 1000) | Growth with bacteria (colony count greater than 1000) | Growth with bacteria (colony count greater than 1000) | Bacterial growth (colony meter)Number greater than 1000) |
| A | Sterile growth | Sterile growth | Sterile growth | Sterile growth |
| B | Sterile growth | Sterile growth | Sterile growth | Sterile growth |
| C | Sterile growth | Sterile growth | Sterile growth | Sterile growth |
As can be seen from the data in Table 1, in the urine samples in the urine collection containers numbered A, B and C, no growth of staphylococcus, escherichia coli, streptococcus and proteus is observed; the colony count of staphylococcus, escherichia coli, streptococcus and proteus in the urine sample in the urine collection container with the number of K is more than 1000;
the results show that in the blank urine sample K without the sample preservative provided by the embodiment of the invention, bacteria are rapidly propagated, and the colony count is greater than the normal count reference value; in a 24-hour urine sample added with the sample preservative prepared in the embodiments 1 to 3, staphylococcus, escherichia coli, streptococcus and proteus do not grow, and the preservative and antibacterial effects are obvious;
test examples 5,
Taking three fresh urine samples of a normal person, wherein each sample is 50mL; one part is used as a reference sample and is numbered as D; 0.5mL of the 24-hour urine sample preservative obtained in example 1 is added into the other part, and the mixture is stirred uniformly and numbered as M; adding 0.5mg of sample preservative according to the existing public proportion of CN110074099 into the third urine sample, wherein the sample is numbered as N;
and (3) respectively taking 1 mL of the urine sample to be detected from the M, N and D samples, and carrying out component detection on the conventional urine, wherein the detection items comprise pH, urine protein, occult blood, specific gravity, glucose and ketone bodies. Urobilinogen, leucocyte, vitamin C, crystal salt of uric acid crystal form salt (sodium, potassium or calcium) of urine crystal, alkaline phosphate, calcium carbonate and the like;
the detection result is that the error of the index data of each detection component between the samples M and D is not more than 1 percent and conforms to the error range allowed by the body fluid detection instrument; in the index data of the detection components between the samples N and D, the ratio of the crystal form salt data of uric acid in the sample N to D is 25 to 30 percent, wherein the abnormal high content of sodium salt is taken as the main factor;
shows that:
compared with a blank urine sample D which is not normally added with the sample agent, the detection results of all components in the M urine sample added with the 24-hour urine sample preservative in the embodiment 1 are basically the same, and the 24-hour urine sample preservative in the embodiment 1 has no influence on the detection of all components and components of urine;
in the M urine sample added with the 24-hour urine sample preservative disclosed in the embodiment 1 of the invention, compared with the urine sample N added with the sample preservative disclosed in the prior art, the uric acid crystal form salt data, especially the sodium salt component detection is more similar to that of a blank sample, and the 24-hour urine sample preservative disclosed in the embodiment 1 of the invention has no influence on the urine ion component detection;
in conclusion, the 24-hour urine sample preservative obtained in the embodiment of the invention does not react with urine protein and uric acid to interfere with the detection result and influence the examination of various ions in the 24-hour urine collection and antibacterial preservation process, and is more favorable for reducing the detection error, providing more accurate and excellent detection result data, being favorable for accurate judgment of diseases and reducing the misdiagnosis probability compared with the existing sample preservative.
The 24-hour urine sample preservative according to other embodiments of the present invention has similar advantageous effects as described above.
The 24-hour urine sample preservative obtained by the technical scheme of other embodiments of the invention also has the similar beneficial effects as described above.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concept. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (9)
1. A24-hour urine sample preservative is characterized in that the preparation raw material comprises 1.5% -4% of isothiazoline-3-ketone mixture; wherein the isothiazolin-3-one mixture comprises 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, and the molar ratio of the 5-chloro-2-methyl-4-isothiazolin-3-one to the 2-methyl-4-isothiazolin-3-one is 2.
2. The 24-hour urine sample preservative according to claim 1, wherein the isothiazolin-3-one mixture is present in a ratio of 2% to 3%.
3. The 24-hour urine sample preservative according to claim 1, wherein the molar ratio of 5-chloro-2-methyl-4-isothiazolin-3-one to 2-methyl-4-isothiazolin-3-one in the isothiazolin-3-one mixture is 2.8 to 1.3.2.
4. The 24-hour urine sample preservative according to claim 1, wherein the raw materials for preparing the 24-hour urine sample preservative further comprise: 0.5-3% of magnesium chloride and 20.0-23.0% of magnesium nitrate.
5. The 24-hour urine sample preservative according to claim 1,
the sample preservative comprises the following raw material components in percentage by weight: the mixture of 5-chloro-2-methyl-4-isothiazoline-3-ketone and 2-methyl-4-isothiazoline-3-ketone accounts for 2-3%, magnesium chloride is 0.5-1.0%, and magnesium nitrate is 21.0-22.0%.
6. The 24-hour urine sample preservative according to claim 1,
the isothiazolin-3-one mixture is used as a main bacteriostatic component of the sample preservative;
the magnesium chloride and the magnesium nitrate are used as stabilizing agents of the sample preservative.
7. A preparation method of a 24-hour urine sample preservative is characterized by comprising the following preparation steps:
s1, preparing corresponding raw materials according to the following weight ratio: 1.5-4% of a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 0.5-1.2% of magnesium chloride and 20.0-22.0% of magnesium nitrate;
and S2, adding a proper amount of water into the components in the S1 at normal temperature, and uniformly mixing to obtain the 24-hour urine sample preservative.
8. Use of the 24-hour urine sample preservative as defined in any one of claims 1 to 5 in preparation of a preservative and urine collection container for urine detection.
9. The method for antiseptically and antibacterially preserving a 24-hour urine sample preservative according to any one of claims 1 to 5, wherein an effective amount of the 24-hour urine sample preservative is applied to a urine collection container.
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