Sample processing and detecting integrated detection tube
Technical Field
The invention relates to the technical field of virus detection devices, in particular to a sample processing and detection integrated detection tube.
Background
With the persistence of novel coronavirus pneumonitis, the current detection methods capable of detecting novel coronavirus pneumonitis viruses are mainly divided into three categories:
Nucleic acid detection: the nucleic acid detection is used for detecting nasal, pharyngeal and anal swabs collected in a human body, lower respiratory tract secretion and alveolar lavage fluid, and the PCR amplification technology can be used for timely detecting results after infection; the nucleic acid detection has higher detection accuracy, but expensive detection equipment is required to be used, and meanwhile, the detection time is long, so that the method has limitations in terms of convenience and timeliness of epidemic prevention.
Antibody detection: antibody detection is used for detecting viruses by using antigen-antibody specific reaction of serum and plasma collected from human bodies, but the detection method is based on the fact that patients can not be detected until the patients are in the onset of diseases; the method can confirm the detection result finally by combining nucleic acid detection even though the detection is rapid, and the detection instrument is matched with the detection instrument, so that the method is only applicable to screening patients infected with novel coronavirus pneumonia in patients suffering from the disease in hospitals and cannot be used by self.
Antigen detection: the antigen detection is used for collecting nasal secretions and oropharynx part secretions of a human body, and detecting viruses by using antigen-antibody specific reaction, the method has the convenience and rapidity of detecting results in a short time after infection, can be used for screening specific crowds as a complementary means of nucleic acid detection, is beneficial to improving early discovery capability, and is an important measure for preventing and controlling current epidemic situation.
However, most antigen detection kits currently consist of a detection reagent card, a sample diluent and a sampling swab, and the use mode is that the sampling swab is used for sampling a sample, then the sampled swab is inserted into a sample extracting solution for fully and uniformly mixing, the diluent is covered with a cover, the diluent mixed with the sample is dripped into a sample adding hole of the reagent card in a quantitative manner, and a detection result is read after waiting for a certain time. The sampling swab, the sample diluent and the reagent card after detection by using the method have risks of biological hazard and virus transmission, and the operation process is relatively complex, so that the method is inconvenient for the old or people with cognitive impairment.
Disclosure of Invention
In view of the foregoing, it is necessary to provide a sample processing and detecting integrated detection tube for solving the problems of complex use and unfavorable popularization and use of the existing antigen detection kit.
The invention provides a sample processing and detecting integrated detection tube. Comprising the following steps: the device comprises a test tube, a sample tube, a middle piece and a clamping piece, wherein the test tube and the sample tube are oppositely arranged at intervals;
A liquid storage tube for containing diluent is inserted into the sample tube, and an opening of the liquid storage tube is arranged opposite to the outside and is provided with a packaging film;
A reagent strip for detecting viruses is arranged in the test tube;
The middle piece is integrally connected with the opening parts of the test tube and the sample tube respectively, and a groove for communicating the test tube and the sample tube is formed in the middle piece;
The clamping piece is inserted into the groove, and a sealing space for communicating the inner cavities of the test tube and the sample tube is formed between the clamping piece and the intermediate piece; a membrane rupture pipe for puncturing the packaging membrane is arranged in the sealing space, one end of the membrane rupture pipe is abutted against the clamping piece, the other end of the membrane rupture pipe is in sliding connection with the sample pipe, and the clamping piece can drive the membrane rupture pipe to puncture the packaging membrane; the clamping piece is provided with a closable insertion hole, and the membrane rupture pipe is communicated with the insertion hole.
Further, a bayonet is arranged on the clamping piece, the bayonet is arranged opposite to the test tube, and the end part of the reagent strip is detachably connected with the bayonet.
Further, a limiting groove body is arranged on one side of the membrane breaking tube, opposite to the test tube, the limiting groove body is inserted into the test tube, and the middle part of the reagent strip is clamped on the limiting groove body.
Further, protruding blocks are arranged on the limiting groove body in a relative mode, and two sides of the reagent strip are in butt joint with the protruding blocks.
Further, the membrane rupture tube is provided with an annular sharp part at one end of the membrane rupture tube opposite to the packaging membrane, and the annular sharp part can puncture the packaging membrane so as to be communicated with the liquid storage tube and the test tube.
Further, an annular bulge is arranged on the clamping piece, two annular depressions arranged at intervals are arranged on the inner wall of the groove, and the annular bulge can be sequentially clamped with the annular depressions; two clamping positions are arranged between the clamping piece and the middle piece, the annular protrusion is clamped with an annular recess in the first clamping position, and the membrane rupture pipe and the packaging membrane are arranged at intervals; in the second clamping position, the annular protrusion is clamped with the other annular recess, and the membrane breaking pipe is communicated with the liquid storage pipe through the packaging membrane.
Further, the clamping piece is provided with a quantitative liquid separating groove body, the quantitative liquid separating groove body is located in the sealing space, and the quantitative liquid separating groove body can control the amount of diluent entering the test tube from the liquid storage tube.
Further, the packaging film is a packaging aluminum foil film, and the packaging film is thermally bonded on the opening of the liquid storage tube.
Further, a detachable hole plug is arranged in the insertion hole, and the hole plug is in interference fit connection with the insertion hole.
Further, an observation window is arranged on the test tube, and the observation window is arranged opposite to the reagent strip.
Compared with the prior art, the invention has the following beneficial effects:
(1) The sample processing and detecting integrated detection tube is provided with the sample tube, the liquid storage tube for containing the diluent is arranged in the sample tube, the opening of the liquid storage tube is arranged at the opposite outer part and is provided with the packaging film, and the packaging film can ensure the relative sealing of the inside of the liquid storage tube, so that the diluent is not leaked during transportation, and the diluent is not polluted.
(2) The invention relates to a sample processing and detecting integrated detection tube which is provided with an intermediate piece, a detection tube and a sample tube, wherein the intermediate piece is integrally connected with opening parts of the detection tube and the sample tube respectively, the intermediate piece forms a groove for communicating the detection tube and the sample tube, and inner cavities of the detection tube and the sample tube are communicated with the groove. The clamping component is inserted in the groove, a sealing space for communicating the inner cavities of the test tube and the sample tube is formed between the clamping component and the middle component, relative circulation of diluent can be realized between the test tube and the sample tube by means of the sealing space, the diluent stained with a virus sample flows into the test tube from the sample tube, and the detection of the virus sample by the reagent strip is assisted. The diluent can be transferred between the test tube and the sample tube without using a titration tool, so that the detection flow is simplified, and the wide applicability of the integrated test tube is improved.
(3) According to the sample processing and detecting integrated detection tube, the membrane breaking tube for puncturing the packaging membrane is arranged in the sealing space, one end of the membrane breaking tube is abutted against the clamping piece, the other end of the membrane breaking tube is connected with the sample tube in a sliding mode, the membrane breaking tube is coaxially arranged with the liquid storage tube in the sample tube, and the clamping piece can drive the membrane breaking tube to puncture the packaging membrane so as to enable diluent in the liquid storage tube to be released into the sealing space. The puncture of the packaging film is not by means of an external tool, so that the clean sterility of the liquid storage tube is always ensured. Be equipped with the closable patchhole on the joint spare, the gas that produces when the tight space of joint spare compression can be released to the patchhole, can also follow the patchhole and insert the swab, makes things convenient for ordinary people's operation, rupture of membranes pipe and patchhole intercommunication, and the swab can directly insert in the stock solution pipe.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute a limitation on the application. In the drawings:
FIG. 1 is an exploded view of the overall invention;
FIG. 2 is a schematic diagram of the connection structure of the test tube, the sample tube and the intermediate member according to the present invention;
FIG. 3 is a schematic diagram of the middleware of the present invention;
FIG. 4 is a schematic structural view of a membrane-rupture tube according to the present invention;
FIG. 5 is a schematic view of the structure of a liquid storage tube in the invention;
FIG. 6 is a schematic view of the overall use structure of the present invention;
In the figure, a test tube 100, a reagent strip 110, a sample tube 200, a liquid storage tube 210, a packaging film 211, a middle piece 300, a groove 310, an annular recess 320, a clamping piece 400, an insertion hole 410, a bayonet 420, an annular protrusion 430, a quantitative liquid separating tank 440, a hole plug 450, a membrane breaking tube 500, a limiting tank 510, a protrusion block 511 and an annular sharp part 520.
Detailed Description
The following detailed description of preferred embodiments of the application is made in connection with the accompanying drawings, which form a part hereof, and together with the description of the embodiments of the application, are used to explain the principles of the application and are not intended to limit the scope of the application.
The embodiment provides a sample is handled and is detected integrative detecting tube relates to virus detection device technical field, mainly through the antigen detection kit that constitutes by structures such as detect reagent card, sample diluent and sampling swab merges into integrated structure, simplifies antigen detection kit's use flow, makes things convenient for ordinary people's use.
Referring to fig. 1 to 6, a sample processing and detecting integrated detection tube in the present embodiment includes: test tube 100 and sample tube 200, intermediate member 300, and clip 400 are disposed in spaced relation to each other.
The liquid storage tube 210 for containing the diluent is inserted into the sample tube 200, the opening of the liquid storage tube 210 is arranged at the opposite outer part and is provided with the packaging film 211, the packaging film 211 can ensure the relative sealing inside the liquid storage tube 210, and the diluent can not leak or be polluted during transportation.
The test tube 100 is internally provided with a reagent strip 110 for detecting viruses, and the reagent strip 110 is prepared by adopting a colloidal gold immunochromatography technology, so that novel coronaviruses in a human nasal swab or a nasopharyngeal swab sample can be detected. When the collected sample contains the novel coronavirus, a mauve line appears at the positions of the C line and the T line after the test of the detection card; when the collected sample is free of the novel coronavirus, only a purple red line appears at the C line position after the test by the detection card.
The intermediate member 300 is integrally connected with the opening portions of the test tube 100 and the sample tube 200, respectively, and the intermediate member 300 forms a groove 310 for communicating the test tube 100 and the sample tube 200, and the inner cavities of the test tube 100 and the sample tube 200 are both communicated with the groove 310. The clamping piece 400 is inserted into the groove 310, a sealing space for communicating the inner cavities of the test tube 100 and the sample tube 200 is formed between the clamping piece 400 and the middle piece 300, relative circulation of diluent can be realized between the test tube 100 and the sample tube 200 by means of the sealing space, diluent stained with virus samples flows into the test tube 100 from the sample tube 200, and the detection of the virus samples by the reagent strips 110 is assisted. The diluent can be transferred between the test tube 100 and the sample tube 200 without using a titration tool, so that the detection process is simplified, and the wide applicability of the integrated detection tube is improved.
The sealed space is provided with a membrane rupture pipe 500 for puncturing the packaging membrane 211, one end of the membrane rupture pipe 500 is abutted with the clamping piece 400, the other end of the membrane rupture pipe 500 is connected with the sample pipe 200 in a sliding mode, the membrane rupture pipe 500 and the liquid storage pipe 210 in the sample pipe 200 are coaxially arranged, and the clamping piece 400 can drive the membrane rupture pipe 500 to puncture the packaging membrane 211 so as to promote the diluent in the liquid storage pipe 210 to be released into the sealed space. The puncturing of the sealing membrane 211 is not by means of external tools, and the clean sterility of the interior of the reservoir 210 is always ensured. The clamping piece 400 is provided with the closable insertion hole 410, the insertion hole 410 can release gas generated when the clamping piece 400 compresses the sealing space, and a swab can be inserted from the insertion hole 410, so that the operation of a common person is facilitated, the membrane breaking tube 500 is communicated with the insertion hole 410, and the swab can be directly inserted into the liquid storage tube 210.
In use, the integrated test tube is held upright in the hand, the insertion hole 410 is opened, and the clamping member 400is pressed downward with thumb force, so that the membrane-rupturing tube 500 punctures the packaging membrane 211. The sampled swab is inserted into the reservoir 210 from the insertion hole 410 and the swab is broken. The insertion hole 410 is closed and the integrated test tube is placed upside down on the table surface, waiting for the completion of the reaction and reading the test result.
Referring to fig. 3, a bayonet 420 is provided on the clamping member 400, the bayonet 420 is disposed opposite to the test tube 100, one end of the reagent strip 110 can be inserted into the bayonet 420, the bayonet 420 clamps the reagent strip 110, the bayonet 420 can fix the reagent strip 110, and the positions of the C line and the T line are ensured to be relatively fixed, so that the observation of ordinary people is facilitated.
Referring to fig. 4, a limiting groove body 510 is disposed on one side of the membrane breaking tube 500 opposite to the test tube 100, the limiting groove body 510 is fixedly connected with the test tube 100, the limiting groove body 510 is inserted into the test tube 100, the middle of the reagent strip 110 is clamped on the limiting groove body 510, the reagent strip 110 can be bound to the limiting groove body 510, the reagent strip 110 is always located in the middle of the test tube 100, bending of the reagent strip 110 is prevented, and the positions of the C line and the T line are ensured to be more convenient for observation. As a further embodiment, the limiting groove 510 is provided with oppositely arranged protruding blocks 511, two sides of the reagent strip 110 are abutted against the protruding blocks 511, and the protruding blocks 511 can firmly fix the reagent strip 110.
The membrane rupture tube 500 is provided with an annular sharp part 520 at one end opposite to the encapsulation membrane 211, the annular sharp part 520 comprises a plurality of sharp teeth surrounding the membrane rupture tube 500, the annular sharp part 520 can puncture the encapsulation membrane 211, and the liquid storage tube 210 is opened, so that the liquid storage tube 210 is communicated with the test tube 100, and diluent with virus can enter the test tube 100 to be contacted with the reagent strip 110.
Referring to fig. 2 and 3, the clamping member 400 is provided with an annular protrusion 430, and two annular recesses 320 disposed at intervals are disposed on the inner wall of the groove 310, and the annular protrusion 430 can be sequentially clamped with the annular recesses 320. In the specific implementation process, two clamping positions are provided between the clamping member 400 and the intermediate member 300, and in the first clamping position, the annular protrusion 430 is clamped with an annular recess 320, the annular recess 320 is close to the notch, the membrane breaking tube 500 and the packaging membrane 211 are arranged at intervals, and the diluent in the liquid storage tube 210 is not in contact with the outside, so that the transportation and storage of the integrated detection tube can be facilitated. The clamping piece 400 is pressed downwards, the clamping piece 400 reaches a second clamping position, the annular protrusion 430 is clamped with the other annular recess 320, the annular recess 320 is far away from the notch, the packaging film 211 is pierced by the film breaking pipe 500 and is inserted into the liquid storage pipe 210, the diluent in the liquid storage pipe 210 can be released, and the test of the reagent strip 110 is assisted.
Referring to fig. 3, the fastening member 400 is provided with a quantitative liquid separating tank 440, the quantitative liquid separating tank 440 is located in the sealed space, and the size of the channel between the liquid storage tube 210 and the test tube 100 can be adjusted by changing the height of the quantitative liquid separating tank 440. After the liquid storage tube 210, the test tube 100 and the clamping piece 400 are relatively sealed, the test tube 100 is inverted, and the height of the diluent in the test tube 100 is equal to the height of the channel under the action of the air pressure in the test tube 100. The height of the channel can be controlled by the quantitative liquid dividing tank 440, so that the immersed length of the reagent strip 110 in the diluent is kept constant, and the accuracy of the test is further improved.
The packaging film 211 is a packaging aluminum foil film, the sealing performance of the packaging aluminum foil film is better than that of a pure plastic, and when the plastic material is seen under a magnifying glass, a plurality of air holes are formed, so that air and water can penetrate. Meanwhile, the aluminum foil composite film has high strength and is not easy to oxidize and crush. In addition, when the heat seal is used, the sealing film 211 and the liquid storage tube 210 are adhered together after being heated and melted, so that the sealing effect is achieved, the aluminum foil is more resistant to high temperature, and the sealing effect is better.
Referring to fig. 1, a detachable plug 450 is disposed in the insertion hole 410, and the plug 450 is connected to the insertion hole 410 in an interference fit manner. In a specific implementation process, the hole plug 450 is a rubber plug, and the rubber plug has elasticity, so that the insertion hole 410 can be better sealed, and the rubber plug is more convenient to disassemble and assemble. The hole plug 450 is connected with the insertion hole 410 in an interference fit manner, so that leakage of diluent can be avoided when the integrated test tube is inverted.
The test tube 100 is provided with an observation window, which is disposed opposite to the reagent strip 110, so that the C-line and T-line of the reagent strip 110 can be observed very conveniently through the observation window.
The working flow is as follows: first, the integrated test tube is vertically held in the hand, the hole plug 450 is pulled out, and the clamping piece 400 is pressed down with thumb force, so that the clamping piece 400 reaches the second clamping position from the first clamping position, and the clamping piece 400 drives the membrane rupture tube 500 to pierce the packaging membrane 211. Next, the sampled swab is inserted into the reservoir 210 from the insertion hole 410 and the swab is broken. Then, the insertion hole 410 is plugged with the hole plug 450 into the insertion hole 410. Finally, the integrated test tube is placed upside down on the table, and the diluted liquid flowing out of the liquid storage tube 210 enters the test tube 100 to wait for the completion of the reaction and read the test result.
While the invention has been described with respect to the preferred embodiments, the scope of the invention is not limited thereto, and any changes or substitutions that would be apparent to those skilled in the art are intended to be included within the scope of the invention.