CN115429839A - A Chinese medicinal composition - Google Patents

Abstract

The invention relates to the technical field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition and its application in the treatment of chronic hepatitis B. Xin, Poria, Scutellaria, Eucommia, Magnolia officinalis, Radix Paeoniae Alba, Rhizoma Chuanxiong, Radix Paeoniae Rubra, Radix Glycyrrhizae, Fructus Citrifolia, Hedyotis; the traditional Chinese medicine composition prepared by the present invention is mainly used for chronic hepatitis B, and can effectively reduce transaminases. The traditional Chinese medicine composition prepared by the invention can prevent the continuous development of chronic hepatitis B at the initial stage, gradually repair liver cells, and finally, continuously restore the normalization of liver function.

Description

A kind of traditional Chinese medicine composition

technical field

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition.

Background technique

Hepatitis B is one of the most harmful diseases to human health in the middle of the 20th century. Viral hepatitis B is highly contagious and has patients all over the world, especially in tropical areas. The incidence is higher and it is harmful to people's health. great. Hepatitis B is an infectious disease that the World Health Organization (WHO) focuses on controlling.

Chronic hepatitis B is a common disease caused by hepatitis B virus. The main manifestations are abnormal liver function, vomiting, loss of appetite, fatigue, etc. Some patients may have symptoms such as jaundice and fever. , Blood transmission, the incidence rate of men is significantly higher than that of women. Chronic hepatitis B belongs to the category of "liver plague", "syndrome", "side pain" and "jaundice" in traditional Chinese medicine. In patients with hepatitis B with blood stasis, the blood stasis in small veins and capillaries cannot be fully absorbed, leading to inflammation and organization, leading to fibrous tissue proliferation.

A large number of clinical practices have proved that traditional Chinese medicine can help improve the clinical symptoms of patients with chronic hepatitis B, promote the recovery of liver function, inhibit the process of liver fibrosis, and delay or prevent the progression of the disease.

Based on this, we propose a Chinese medicine composition, hoping to solve the deficiencies in the prior art.

Contents of the invention

(1) Solved technical problems

Aiming at the deficiencies of the prior art, the invention provides a traditional Chinese medicine composition.

(2) Technical solution

To achieve the above object, the present invention provides the following technical solutions:

A traditional Chinese medicine composition, which is prepared from the following ingredients in parts by weight: 13-15 parts of antelope bone, 10-12 parts of astragalus, 8-14 parts of angelica, 12-16 parts of Bupleurum, 2-5 parts of salvia miltiorrhiza, Daqing 1-3 parts of leaves, 4-10 parts of Asarum, 10-20 parts of Poria cocos, 30-35 parts of Scutellaria baicalensis, 11-16 parts of Eucommia ulmoides, 8-14 parts of Magnolia officinalis, 6-12 parts of white peony root, 3-7 parts of Chuanxiong , 5-10 parts of Radix Paeoniae Rubra, 12-18 parts of Licorice Root, 30-35 parts of Citrus Aurantium, and 1-3 parts of Hedyotis.

As a further technical solution, the mixed weight ratio of the Scutellaria baicalensis and Fructus Citrus Fructus is 1:1.

As a further technical solution, the preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong and Bupleurum are mixed together, then fumigated for 20-25min to obtain the fumigation material;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water of 10-12 times its mass to the mixture obtained above, soak it for 10-15 minutes, then boil it until it boils, then add the above-mentioned fumigating material, after stirring evenly, simmer for 2-3 hours;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution;

(7) Grinding Scutellaria baicalensis and Fructus Aurantii Fructus Aurantii, and then using ethanol solution to reflux and stir it for 2 hours, cooling to room temperature and continuing to stir for 12 hours, centrifuging to get the supernatant;

(8) Put the supernatant obtained above into a dialysis bag for dialysis treatment, and spray-dry the dialysate obtained by dialysis to obtain a mixed extract;

(9) Add sodium sulfate to the hydrochloric acid solution, stir until dissolved, then add trichloroacetic acid and barium chloride, and continue stirring to obtain a treatment solution;

(10) Add the mixed extracts to the treatment solution, adjust the temperature to 50-60°C, heat and stir the reaction for 1-2 hours to obtain the reaction solution, adjust the pH of the reaction solution to neutral, and then carry out in the dialysis bag Dialysis treatment, the dialysate obtained by dialysis is spray-dried to obtain a drug compound;

(11) Add the drug compound into the concentrated drug solution, stir evenly, and the product is ready.

As a further technical solution: the fumigation treatment in the step (2) is: use water vapor to fumigate Folium Folium Folium, Rhizoma Chuanxiong and Bupleurum chinensis, wherein the temperature of the water vapor in the fumigation treatment is 100°C.

As a further technical solution, the immersion treatment in step (4) is: the immersion treatment is carried out at normal temperature.

As a further technical scheme, the solid-liquid ratio of Scutellaria baicalensis described in step (7), Fructus Fructus Citrus Citrifolia and ethanol solution mixing is 1:10-12;

Wherein, the mass fraction of the ethanol solution is 70%.

As a further technical solution: the dialysis bag described in step (8) is a dialysis bag with a molar mass of 3500DA;

The dialysis bag described in step (10) is a dialysis bag with a molar mass of 3500DA.

As a further technical scheme: in the step (9), the mixed mass ratio of sodium sulfate and hydrochloric acid solution is 1-3:25;

The concentration of the hydrochloric acid solution is 0.1mol/L;

The mixing mass ratio of hydrochloric acid solution, trichloroacetic acid and barium chloride is 25:1-3:1.

The traditional Chinese medicine composition is applied to the treatment of chronic hepatitis B.

Licorice is a perennial herbaceous plant in the leguminous family, and its roots and rhizomes are used as medicine. It is a tonic Chinese herbal medicine. Licorice is flat in nature and sweet in taste. It has the functions of invigorating the spleen and replenishing qi, clearing heat and detoxification, eliminating phlegm and relieving cough, relieving spasm and relieving pain, and harmonizing various medicines. Pain, carbuncle sores, relieving drug toxicity and potency.

Angelica: sweet, pungent, warm, returns liver, heart, spleen meridian. Efficacy: Tonify blood and activate blood, regulate menstruation and relieve pain, moisten dryness and smooth intestines, treat anemia, irregular menstruation, amenorrhea, dysmenorrhea, metrorrhagia, postpartum abdominal pain, blood deficiency constipation, bruises, carbuncle sores.

Asarum (AsarumsieboldiiMiq), a perennial herb of Aristolochiaceae and Asarum genus, has the functions of relieving exterior cold, expelling wind and relieving pain, clearing the orifices, warming the lungs and transforming drink.

Radix Paeoniae Rubra (Han Shao): Bitter, slightly cold, returns to Liver Meridian. Efficacy: clearing heat and cooling blood, dispelling blood stasis and relieving pain, used for febrile disease and heat in the blood, can clear blood stagnation, this product can dispel blood stasis, promote blood circulation and stimulate menstruation.

Poria cocos, nature and flavor: sweet, light, flat, has the effect of diuresis and diuresis, invigorating the spleen and stomach, calming the mind and calming the nerves, used to treat dysuria, edema and fullness, cough with phlegm, vomiting, spleen deficiency, lack of food, and diarrhea , palpitations, insomnia, forgetfulness, seminal emission and white turbidity.

Radix Paeoniae Alba, nature and flavor: bitter, sour, slightly cold, nourishing blood and nutrient, relieving spasm and relieving pain, astringing yin and calming the liver, used to treat irregular menstruation, abdominal pain during menstruation, metrorrhagia, spontaneous sweating, night sweats, flanks and abdomen Pain, limb spasm, headache, dizziness and other symptoms.

The traditional Chinese medicine composition prepared by the present invention can treat and improve chronic hepatitis B by improving immunity, and at the same time has the function of anti-cancer and enhancing body immunity, has anti-proliferation of pre-cancerous mutant cells, and can first delay the progress of chronic hepatitis B, Afterwards, it improves and repairs liver cells, improves liver function, and promotes its return to normal function.

The traditional Chinese medicine composition provided by the present invention for the treatment of chronic hepatitis B is the result of the inventor's repeated clinical practice and multiple optimizations of the formula, and finally obtains a drug combination prepared by combining different combinations of Chinese medicine decoctions. The combination of the two can greatly improve the effect of treating chronic hepatitis B.

The invention provides a variety of traditional Chinese medicine compositions for treating chronic hepatitis B. The raw materials of the traditional Chinese medicine composition include antelope bone, astragalus, angelica, bupleurum, salvia miltiorrhiza, Folium Folium, asarum, poria cocos, Eucommia, thick Park, white peony, Chuanxiong, red peony, licorice, hedyotis, aurantium, scutellaria baicalensis, and antelope bone, astragalus, angelica, Bupleurum, salvia miltiorrhiza, Daqingye, Asarum, Poria, Eucommia, Magnolia officinalis, white The water decoction prepared by combining peony, Chuanxiong, red peony, licorice, and Hedyotis, and the drug compound prepared by Citrus aurantium and Scutellaria baicalensis can not only effectively relieve various symptoms of patients with chronic hepatitis B, but also repair the liver. Cells, reduce the annual incidence of liver cirrhosis in patients with chronic hepatitis B hepatitis and the annual incidence of primary liver cancer in patients with hepatitis B-related compensated liver cirrhosis.

Folium Folium has an auxiliary anti-inflammatory effect, and the drug compound can effectively enhance cellular immunity and humoral immunity, effectively promote the regeneration and repair of liver cells, enhance immunity, and at the same time effectively inhibit liver cell damage and improve Liver function, promote liver function to return to normal. The drug compound can not only resist oxidation, regulate immunity, fight cancer, enhance the function of liver detoxification, but also protect the liver. The drug compound can repair damaged liver cells to a certain extent, thereby Reduce transaminase and protect the liver.

After long-term medication tracking, the traditional Chinese medicine composition of the present invention reduces the annual incidence rate of liver cirrhosis in patients with chronic hepatitis B from 12.5% to 0.8%, and the annual incidence rate of primary liver cancer in patients with hepatitis B-related compensated liver cirrhosis From 5.8% to 1.1%.

Astragalus has a protective effect on the liver and can enhance the smooth muscle tension of the small intestine. Astragalus has antibacterial and antiviral effects. It also has protective effects on hematopoietic and immune systems.

(3) Beneficial effects

Compared with the prior art, the present invention provides a traditional Chinese medicine composition, which has the following beneficial effects:

The traditional Chinese medicine composition prepared by the present invention is mainly used for chronic hepatitis B, and can effectively reduce transaminase. Taking the traditional Chinese medicine composition prepared by the present invention can prevent the continuous development of chronic hepatitis B at the initial stage, and gradually repair liver cells , and finally, the constant restoration of liver function normalization.

Description of drawings

Fig. 1 is the histogram of embodiment and comparative example clinical symptom and sign situation improvement rate;

Fig. 2 is a histogram of liver function ALT before and after treatment in the embodiment and the comparative example.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The following are specific examples:

Example 1

A traditional Chinese medicine composition, prepared from the following ingredients in parts by weight: 13 parts of antelope bone, 10 parts of astragalus, 8 parts of angelica, 12 parts of Bupleurum, 2 parts of salvia miltiorrhiza, 1 part of Daqingye, 4 parts of Asarum, Poria cocos 10 parts, 30 parts of Scutellaria baicalensis, 11 parts of Eucommia ulmoides, 8 parts of Magnolia officinalis, 6 parts of white peony, 3 parts of Chuanxiong, 5 parts of red peony, 12 parts of licorice, 30 parts of Citrus aurantium, 1 part of Hedyotis The actual mixing weight ratio is 1:1.

The preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong, and Bupleurum are mixed together, and then fumigation is carried out for 20 minutes to obtain a fumigation material, and water vapor is used to fumigate Folium Folium, Rhizoma Chuanxiong, and Bupleurum, wherein the water vapor temperature of the fumigation is at 100°C;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water 10 times its mass to the mixture obtained above, soak it for 10 minutes, then boil it until it boils, then add the above-mentioned fumigation material, stir evenly, simmer for 2 hours, and soak it at room temperature Soaking treatment;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution;

(7) Pulverize Scutellaria baicalensis and Fructus Aurantii Fructus, then reflux and stir it with ethanol solution for 2h, continue to stir for 12h after cooling to room temperature, centrifuge to get the supernatant, the solid-liquid ratio of Scutellaria baicalensis, Fructus Fructus Fructus Fructus Citrifolia and ethanol solution is mixed as 1:10;

Wherein, the ethanol solution mass fraction is 70%;

(8) The supernatant obtained above is placed in a dialysis bag with a molar mass of 3500DA for dialysis treatment, and the dialysate obtained by the dialysis is spray-dried to obtain a mixed extract;

(9) Add sodium sulfate to the hydrochloric acid solution, stir until dissolved, then add trichloroacetic acid and barium chloride, continue stirring to obtain a treatment solution, the mixing mass ratio of sodium sulfate and hydrochloric acid solution is 1:25;

The concentration of the hydrochloric acid solution is 0.1mol/L;

The mixing mass ratio of hydrochloric acid solution, trichloroacetic acid and barium chloride is 25:1:1;

(10) Add the mixed extracts to the treatment solution, adjust the temperature to 50°C, heat and stir the reaction for 1 hour to obtain a reaction solution, adjust the pH of the reaction solution to neutral, and then place it in a dialysis bag with a molar mass of 3500DA Carry out dialysis treatment, and the dialysate obtained by dialysis is spray-dried to obtain a drug compound;

(11) Add the drug compound into the concentrated drug solution, stir evenly, and the product is ready.

Example 2

A traditional Chinese medicine composition, which is prepared from the following ingredients in parts by weight: 14 parts of antelope bone, 11 parts of astragalus, 10 parts of angelica, 13 parts of Bupleurum, 4 parts of Salvia miltiorrhiza, 2 parts of Daqingye, 5 parts of Asarum, Poria cocos 12 parts, 31 parts of Scutellaria baicalensis, 12 parts of Eucommia ulmoides, 10 parts of Magnolia officinalis, 8 parts of white peony, 4 parts of Chuanxiong, 6 parts of red peony, 14 parts of licorice, 31 parts of Citrus aurantii, 2 parts of Hedyotis. The actual mixing weight ratio is 1:1.

The preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong, and Bupleurum are mixed together, and then fumigated for 22 minutes to obtain a fumigation material, and water vapor is used to fumigate Folium Folium, Rhizoma Chuanxiong, and Bupleurum, wherein the water vapor temperature of the fumigation is at 100°C;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water of 11 times its mass to the mixture obtained above, soak it for 12 minutes, then boil it until it boils, then add the above-mentioned fumigation material, stir evenly, simmer for 2.5 hours, and soak it at room temperature Soaking treatment;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution;

(7) Pulverize Scutellaria baicalensis and Fructus Aurantii Fructus, then reflux and stir it with ethanol solution for 2h, continue to stir for 12h after cooling to room temperature, centrifuge to get the supernatant, the solid-liquid ratio of Scutellaria baicalensis, Fructus Fructus Fructus Fructus Citrifolia and ethanol solution is mixed as 1:11;

Wherein, the ethanol solution mass fraction is 70%;

(8) The supernatant obtained above is placed in a dialysis bag with a molar mass of 3500DA for dialysis treatment, and the dialysate obtained by the dialysis is spray-dried to obtain a mixed extract;

(9) Add sodium sulfate to the hydrochloric acid solution, stir until dissolved, then add trichloroacetic acid and barium chloride, continue stirring to obtain a treatment solution, the mixing mass ratio of sodium sulfate and hydrochloric acid solution is 2:25;

The concentration of the hydrochloric acid solution is 0.1mol/L;

The mixed mass ratio of hydrochloric acid solution, trichloroacetic acid and barium chloride is 25:2:1;

(10) Add the mixed extracts to the treatment solution, adjust the temperature to 55°C, heat and stir for 1.5 hours to obtain a reaction solution, adjust the pH of the reaction solution to neutral, and then place it in a dialysis bag with a molar mass of 3500DA Carry out dialysis treatment, and the dialysate obtained by dialysis is spray-dried to obtain a drug compound;

(11) Add the drug compound into the concentrated drug solution, stir evenly, and the product is ready.

Example 3

A traditional Chinese medicine composition, which is prepared from the following ingredients in parts by weight: 14 parts of antelope bone, 11 parts of astragalus, 10 parts of angelica, 14 parts of Bupleurum, 3 parts of Salvia miltiorrhiza, 2 parts of Daqingye, 5 parts of Asarum, Poria cocos 15 parts, 32 parts of Scutellaria baicalensis, 14 parts of Eucommia ulmoides, 12 parts of Magnolia officinalis, 8 parts of white peony, 4 parts of Chuanxiong, 6 parts of red peony, 15 parts of licorice, 32 parts of Citrus aurantium, 2 parts of Hedyotis. The mixing weight ratio is 1:1.

The preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong, and Bupleurum are mixed together, and then fumigated for 22 minutes to obtain a fumigation material, and water vapor is used to fumigate Folium Folium, Rhizoma Chuanxiong, and Bupleurum, wherein the water vapor temperature of the fumigation is at 100°C;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water of 11 times its mass to the mixture obtained above, soak it for 12 minutes, then boil it until it boils, then add the above-mentioned fumigation material, stir evenly, simmer for 2.5 hours, and soak it at room temperature Soaking treatment;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution;

(7) Pulverize Scutellaria baicalensis and Fructus Aurantii Fructus, then reflux and stir it with ethanol solution for 2h, continue to stir for 12h after cooling to room temperature, centrifuge to get the supernatant, the solid-liquid ratio of Scutellaria baicalensis, Fructus Fructus Fructus Fructus Citrifolia and ethanol solution is mixed as 1:11;

Wherein, the ethanol solution mass fraction is 70%;

(8) The supernatant obtained above is placed in a dialysis bag with a molar mass of 3500DA for dialysis treatment, and the dialysate obtained by the dialysis is spray-dried to obtain a mixed extract;

(9) Add sodium sulfate to the hydrochloric acid solution, stir until dissolved, then add trichloroacetic acid and barium chloride, continue stirring to obtain a treatment solution, the mixing mass ratio of sodium sulfate and hydrochloric acid solution is 2:25;

The concentration of the hydrochloric acid solution is 0.1mol/L;

The mixed mass ratio of hydrochloric acid solution, trichloroacetic acid and barium chloride is 25:2:1;

(10) Add the mixed extracts to the treatment solution, adjust the temperature to 55°C, heat and stir for 1.5 hours to obtain a reaction solution, adjust the pH of the reaction solution to neutral, and then place it in a dialysis bag with a molar mass of 3500DA Carry out dialysis treatment, and the dialysate obtained by dialysis is spray-dried to obtain a drug compound;

(11) Add the drug compound into the concentrated drug solution, stir evenly, and the product is ready.

Example 4

A traditional Chinese medicine composition, prepared from the following ingredients in parts by weight: 14 parts of antelope bone, 11 parts of astragalus, 12 parts of angelica, 15 parts of Bupleurum, 4 parts of salvia miltiorrhiza, 2 parts of Daqingye, 5 parts of Asarum, Poria cocos 18 parts, 34 parts of Scutellaria baicalensis, 15 parts of Eucommia ulmoides, 12 parts of Magnolia officinalis, 10 parts of white peony, 5 parts of Chuanxiong, 8 parts of red peony, 15 parts of licorice, 34 parts of Citrus aurantium, 2 parts of Hedyotis The actual mixing weight ratio is 1:1.

The preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong, and Bupleurum are mixed together, and then fumigated for 24 minutes to obtain a fumigation material, and water vapor is used to fumigate Folium Folium, Rhizoma Chuanxiong, and Bupleurum, wherein the water vapor temperature of the fumigation is at 100°C;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water of 11 times its mass to the mixture obtained above, soak it for 12 minutes, then boil it until it boils, then add the above-mentioned fumigation material, stir evenly, simmer for 2.5 hours, and soak it at room temperature Soaking treatment;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution;

(7) Pulverize Scutellaria baicalensis and Fructus Aurantii Fructus, then reflux and stir it with ethanol solution for 2h, continue to stir for 12h after cooling to room temperature, centrifuge to get the supernatant, the solid-liquid ratio of Scutellaria baicalensis, Fructus Fructus Fructus Fructus Citrifolia and ethanol solution is mixed as 1:11;

Wherein, the ethanol solution mass fraction is 70%;

(8) The supernatant obtained above is placed in a dialysis bag with a molar mass of 3500DA for dialysis treatment, and the dialysate obtained by the dialysis is spray-dried to obtain a mixed extract;

(9) Add sodium sulfate to the hydrochloric acid solution, stir until dissolved, then add trichloroacetic acid and barium chloride, continue stirring to obtain a treatment solution, the mixing mass ratio of sodium sulfate and hydrochloric acid solution is 2:25;

The concentration of the hydrochloric acid solution is 0.1mol/L;

The mixed mass ratio of hydrochloric acid solution, trichloroacetic acid and barium chloride is 25:2:1;

(10) Add the mixed extract to the treatment solution, adjust the temperature to 50-60°C, heat and stir the reaction for 1-2 hours to obtain the reaction solution, adjust the pH of the reaction solution to neutral, and then adjust the pH of the reaction solution to 3500DA Carry out dialysis treatment in the dialysis bag of dialysis, the dialysate obtained by dialysis is spray-dried to obtain the drug compound;

(11) Add the drug compound into the concentrated drug solution, stir evenly, and the product is ready.

Example 5

A traditional Chinese medicine composition, prepared from the following ingredients in parts by weight: 15 parts of antelope bone, 12 parts of astragalus, 14 parts of angelica, 16 parts of Bupleurum, 5 parts of salvia miltiorrhiza, 3 parts of Daqingye, 10 parts of Asarum, Poria cocos 20 parts, 35 parts of Scutellaria baicalensis, 16 parts of Eucommia ulmoides, 14 parts of Magnolia officinalis, 12 parts of white peony, 7 parts of Chuanxiong, 10 parts of red peony, 18 parts of licorice, 35 parts of Citrus aurantium, 3 parts of Hedyotis, The actual mixing weight ratio is 1:1.

The preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong, and Bupleurum are mixed together, and then fumigation is carried out for 25 minutes to obtain a fumigation material, and water vapor is used to fumigate Folium Folium, Rhizoma Chuanxiong, and Bupleurum, wherein the water vapor temperature of the fumigation is at 100°C;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water of 12 times its mass to the mixture obtained above, soak it for 15 minutes, then boil it until it boils, then add the above-mentioned fumigating material, after stirring evenly, simmer for 3 hours, and the soaking treatment is carried out at room temperature Soaking treatment;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution;

(7) Pulverize Scutellaria baicalensis and Fructus Aurantii Fructus, then reflux and stir it with ethanol solution for 2h, continue to stir for 12h after cooling to room temperature, centrifuge to get the supernatant, the solid-liquid ratio of Scutellaria baicalensis, Fructus Fructus Fructus Fructus Citrifolia and ethanol solution is mixed as 1:12;

Wherein, the ethanol solution mass fraction is 70%;

(8) The supernatant obtained above is placed in a dialysis bag with a molar mass of 3500DA for dialysis treatment, and the dialysate obtained by the dialysis is spray-dried to obtain a mixed extract;

(9) Add sodium sulfate to the hydrochloric acid solution, stir until dissolved, then add trichloroacetic acid and barium chloride, continue stirring to obtain a treatment solution, the mixing mass ratio of sodium sulfate and hydrochloric acid solution is 3:25;

The concentration of the hydrochloric acid solution is 0.1mol/L;

The mixing mass ratio of hydrochloric acid solution, trichloroacetic acid and barium chloride is 25:3:1;

(10) Add the mixed extracts to the treatment solution, adjust the temperature to 60°C, heat and stir the reaction for 2 hours to obtain a reaction solution, adjust the pH of the reaction solution to neutral, and then place it in a dialysis bag with a molar mass of 3500DA Carry out dialysis treatment, and the dialysate obtained by dialysis is spray-dried to obtain a drug compound;

(11) Add the drug compound into the concentrated drug solution, stir evenly, and the product is ready.

Comparative example 1:

A traditional Chinese medicine composition, prepared from the following ingredients in parts by weight: 13 parts of antelope bone, 10 parts of astragalus, 8 parts of angelica, 12 parts of Bupleurum, 2 parts of salvia miltiorrhiza, 1 part of Daqingye, 4 parts of Asarum, Poria cocos 10 parts, 11 parts of Eucommia, 8 parts of Magnolia officinalis, 6 parts of white peony, 3 parts of Chuanxiong, 5 parts of red peony, 12 parts of licorice, and 1 part of Hedyotis.

The preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong, and Bupleurum are mixed together, and then fumigation is carried out for 20 minutes to obtain a fumigation material, and water vapor is used to fumigate Folium Folium, Rhizoma Chuanxiong, and Bupleurum, wherein the water vapor temperature of the fumigation is at 100°C;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water 10 times its mass to the mixture obtained above, soak it for 10 minutes, then boil it until it boils, then add the above-mentioned fumigation material, stir evenly, simmer for 2 hours, and soak it at room temperature Soaking treatment;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) Concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution.

Comparative example 2:

A traditional Chinese medicine composition, prepared from the following ingredients in parts by weight: 13 parts of antelope bone, 10 parts of astragalus, 8 parts of angelica, 12 parts of Bupleurum, 2 parts of salvia miltiorrhiza, 1 part of Daqingye, 4 parts of Asarum, Poria cocos 10 parts, 30 parts of Scutellaria baicalensis, 11 parts of Eucommia ulmoides, 8 parts of Magnolia officinalis, 6 parts of white peony, 3 parts of Chuanxiong, 5 parts of red peony, 12 parts of licorice, and 1 part of Hedyotis.

The preparation method of the Chinese medicine composition is:

(1) Weigh antelope bone, astragalus root, angelica, bupleurum root, salvia miltiorrhiza, Folium chinensis, asarum, poria cocos, scutellaria baicalensis, eucommia ulmoides, Magnolia officinalis officinalis, white peony root, Chuanxiong root, red peony root, licorice root, citrus aurantium, Hedyotis;

(2) Folium Folium Folium, Rhizoma Chuanxiong, and Bupleurum are mixed together, and then fumigation is carried out for 20 minutes to obtain a fumigation material, and water vapor is used to fumigate Folium Folium, Rhizoma Chuanxiong, and Bupleurum, wherein the water vapor temperature of the fumigation is at 100°C;

(3) Add antelope bone, astragalus, angelica, salvia miltiorrhiza, asarum, poria cocos, eucommia ulmoides, magnolia officinalis, white peony root, red peony root, licorice root, and hedyotis into the mixer in order to mix evenly, and then take it out to obtain a mixed material;

(4) Add clear water 10 times its mass to the mixture obtained above, soak it for 10 minutes, then boil it until it boils, then add the above-mentioned fumigation material, stir evenly, simmer for 2 hours, and soak it at room temperature Soaking treatment;

(5) After the decoction time ends, filter to obtain the filtrate;

(6) concentrating the filtrate to 50% of the original volume to obtain a concentrated medicinal solution;

(7) Scutellaria baicalensis is pulverized, then it is refluxed and stirred with ethanol solution for 2 hours, after cooling to room temperature, continue to stir for 12 hours, and the supernatant is obtained by centrifugation. The solid-liquid ratio of Scutellaria baicalensis and ethanol solution is 1:10;

Wherein, the ethanol solution mass fraction is 70%;

(8) The supernatant obtained above is placed in a dialysis bag with a molar mass of 3500DA for dialysis treatment, and the dialysate obtained by the dialysis is spray-dried to obtain a mixed extract;

(9) Add sodium sulfate to the hydrochloric acid solution, stir until dissolved, then add trichloroacetic acid and barium chloride, continue stirring to obtain a treatment solution, the mixing mass ratio of sodium sulfate and hydrochloric acid solution is 1:25;

The concentration of the hydrochloric acid solution is 0.1mol/L;

The mixing mass ratio of hydrochloric acid solution, trichloroacetic acid and barium chloride is 25:1:1;

(10) Add the mixed extracts to the treatment solution, adjust the temperature to 50°C, heat and stir the reaction for 1 hour to obtain a reaction solution, adjust the pH of the reaction solution to neutral, and then place it in a dialysis bag with a molar mass of 3500DA Carry out dialysis treatment, and the dialysate obtained by dialysis is spray-dried to obtain a drug compound;

(11) Add the drug compound into the concentrated drug solution, stir evenly, and the product is ready.

Choose 20～60 years old, voluntarily and can cooperate to treat 245 cases of chronic hepatitis B, divide into 7 groups at random, each group of 35 people, adopt embodiment and comparative example Chinese medicine composition to carry out test respectively, compare:

3 treatment methods

Three times a day, each dosage is 150ml, and the course of treatment is 6 months;

Observation indicators:

Clinical symptoms and signs: fatigue, loss of appetite, discomfort in the liver area, mild hepatomegaly or improvement rate of percussion pain in the liver area.

Each index of liver function ALT, AST, TB, ALB of each group of embodiment 1 and comparative example changes before and after treatment, gets the mean value:

Table 1

Improvement rate of clinical symptoms and signs % Example 1 97.14 Example 2 100 Example 3 100 Example 4 97.14 Example 5 94.28 Comparative example 1 57.14 Comparative example 2 71.43

As can be seen from Table 1, the traditional Chinese medicine composition prepared by the present invention can greatly improve the patient's fatigue, loss of appetite, discomfort in the liver area, mild hepatomegaly or percussion pain in the liver area;

Table 2

It can be seen from Table 2 that the traditional Chinese medicine composition prepared by the method of the present invention can greatly improve the indicators of liver function ALT, AST, TB, and ALB.

Typical cases:

1 Chen Moumou, female, 38 years old, the patient had distending pain in the right flank, loss of appetite, fatigue and fatigue, dark complexion, occasional involvement in the back, dysmenorrhea, and the hospital examination report showed abnormal liver function, hepatomegaly under the ribs 1.8cm, was diagnosed as "chronic hepatitis B", symptoms at the time of treatment: dry mouth, bitter mouth, heavy breath, rib pain, fatigue.

Examination: complexion black and yellow, large liver and ribs 1.82cm below, spleen untouched, dull complexion, dark red tongue with yellow greasy coating, pulse strings and pulses, liver function test was alanine aminotransferase 600 units, five items of hepatitis B: HBsAg(+ ), anti-HBs(-), HBeAg(+), anti-HBe(-), anti-HBc(-).

Dialectical: active chronic hepatitis B.

Method for the treatment of: take Chinese medicine of the present invention continuously, each 150ml, every day 3 times, continuous 6 months.

Efficacy: After investigation, various liver function indicators returned to normal indicators.

Fig. 1 is the histogram of the improvement rate of the clinical symptoms and signs of the embodiment and the comparative example.

The histogram of the liver function ALT before and after treatment of the embodiment and the comparative example is shown in FIG. 2 . Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes, modifications and substitutions can be made to these embodiments without departing from the principle and spirit of the present invention. and modifications, the scope of the invention is defined by the appended claims and their equivalents.

Claims (9)

1. A traditional Chinese medicine composition is characterized by being prepared from the following components in parts by weight: 13-15 parts of antelope bone, 10-12 parts of astragalus root, 8-14 parts of angelica, 12-16 parts of radix bupleuri, 2-5 parts of salvia miltiorrhiza, 1-3 parts of folium isatidis, 4-10 parts of asarum, 10-20 parts of poria cocos, 30-35 parts of scutellaria baicalensis, 11-16 parts of eucommia ulmoides, 8-14 parts of mangnolia officinalis, 6-12 parts of radix paeoniae alba, 3-7 parts of ligusticum wallichii, 5-10 parts of red paeony root, 12-18 parts of liquorice, 30-35 parts of immature bitter orange and 1-3 parts of oldenlandia diffusa.

2. The traditional Chinese medicine composition of claim 1, wherein the ratio of the scutellaria baicalensis to the immature bitter orange in parts by weight is 1.

3. The traditional Chinese medicine composition of claim 2, wherein the preparation method of the traditional Chinese medicine composition comprises the following steps:

(1) Weighing the cornu saigae tataricae, the astragalus membranaceus, the angelica sinensis, the radix bupleuri, the salvia miltiorrhiza, the folium isatidis, the asarum, the poria cocos, the scutellaria baicalensis, the eucommia ulmoides, the mangnolia officinalis, the radix paeoniae alba, the ligusticum wallichii, the radix paeoniae rubra, the liquorice, the immature bitter orange and the oldenlandia diffusa in parts by weight;

(2) Mixing folium Isatidis, rhizoma Ligustici Chuanxiong, and bupleuri radix, and fumigating for 20-25min to obtain fumigant;

(3) Adding cornu Saigae Tataricae, radix astragali, radix Angelicae sinensis, saviae Miltiorrhizae radix, herba asari, poria, eucommiae cortex, cortex Magnolia officinalis, radix Paeoniae alba, radix Paeoniae Rubra, glycyrrhrizae radix, and herba Hedyotidis Diffusae into a mixer in sequence, mixing, and taking out to obtain mixture;

(4) Adding clear water 10-12 times of the weight of the mixture into the mixture, soaking for 10-15min, decocting for boiling, adding the fumigation material, stirring, and decocting with slow fire for 2-3 hr;

(5) After the decoction time is finished, filtering to obtain filtrate;

(6) Concentrating the filtrate to 50% of the original volume to obtain concentrated medicinal liquid;

(7) Pulverizing Scutellariae radix and fructus Aurantii Immaturus, reflux-stirring with ethanol solution for 2 hr, cooling to room temperature, stirring for 12 hr, centrifuging, and collecting supernatant;

(8) Loading the obtained supernatant into a dialysis bag for dialysis treatment, and spray drying the dialyzate obtained by dialysis to obtain a mixed extract;

(9) Adding sodium sulfate into hydrochloric acid solution, stirring until the sodium sulfate is dissolved, adding trichloroacetic acid and barium chloride, and continuously stirring to obtain a treatment solution;

(10) Adding the mixed extract into the treatment solution, adjusting the temperature to 50-60 deg.C, maintaining the temperature, stirring, reacting for 1-2 hr to obtain reaction solution, adjusting pH to neutral, dialyzing in dialysis bag, and spray drying the dialyzed solution to obtain medicinal ingredient;

(11) Adding the medicinal compounding agent into the concentrated medicinal liquid, and stirring.

4. The traditional Chinese medicine composition according to claim 3, wherein: the fumigation treatment in the step (2) comprises the following steps: fumigating folium Isatidis, rhizoma Ligustici Chuanxiong, and bupleuri radix with steam at 100 deg.C.

5. The traditional Chinese medicine composition according to claim 3, wherein the soaking treatment in step (4) is: the soaking treatment is carried out at normal temperature.

6. The traditional Chinese medicine composition according to claim 3, wherein in the step (7), the ratio of the mixture of the scutellaria baicalensis, the immature bitter orange and the ethanol solution is 1;

wherein the mass fraction of the ethanol solution is 70%.

7. The traditional Chinese medicine composition according to claim 3, wherein: the dialysis bag in the step (8) is a dialysis bag with the molar mass of 3500 DA;

the dialysis bag in the step (10) is a dialysis bag with a molar mass of 3500 DA.

8. The traditional Chinese medicine composition according to claim 3, wherein: in the step (9), the mixing mass ratio of the sodium sulfate to the hydrochloric acid solution is 1-3:25;

the concentration of the hydrochloric acid solution is 0.1mol/L;

the mixing mass ratio of the hydrochloric acid solution to the trichloroacetic acid to the barium chloride is 25.

9. The use of the composition according to any one of claims 1-8 for the treatment of chronic hepatitis b.

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