CN115399313A

CN115399313A - Immune cell cryopreserving cooling device

Info

Publication number: CN115399313A
Application number: CN202211341859.XA
Authority: CN
Inventors: 王爱婷; 王学动; 闫奔; 张洋; 任美佳; 李松; 杜锦梅
Original assignee: Maisaikele Shandong Life Science Co ltd
Current assignee: Maisaikele Shandong Life Science Co ltd
Priority date: 2022-10-31
Filing date: 2022-10-31
Publication date: 2022-11-29
Anticipated expiration: 2042-10-31
Also published as: CN115399313B

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to an immune cell cryopreservation cooling device which comprises a base and a storage assembly arranged above the base; put into the test tube inslot with the test tube, to seal the lid to jar body sealed back, and cross the liquid nitrogen intracavity and pour into microthermal liquid nitrogen into, lower the temperature to the internal test tube of jar through the liquid nitrogen, carry out cryo preservation to the cell in the test tube, it takes place to rotate to drive pivot and swivel mount through the control motor start-up, the internal test tube of jar that drives the swivel mount surface rotates, rotate the mixing efficiency of accelerating cell and frozen stock liquid through the test tube, guarantee cell and frozen stock liquid can the flash mixed, prevent that the cell from breaking the death at the cooling in-process, make the sliding sleeve drive the hinge bar when the sliding surface of transfer line and remove, it rocks with articulated seat slope about the swivel mount to drive jar body through the hinge lug of bottom, the jar body under the cooperation rotation state of being convenient for, further the mixing efficiency to the cell in the test tube and frozen stock liquid.

Description

Immune cell cryopreserving cooling device

Technical Field

The invention belongs to the technical field of biological medicines, and particularly relates to an immune cell cryopreservation cooling device.

Background

The immune cells refer to cells involved in or related to immune response, including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells and the like, can be divided into a plurality of types, various immune cells play an important role in a human body, the immune cells are generally preserved by a cryopreservation device, the cells are preserved at low temperature of liquid nitrogen at-196 ℃, the cells can be temporarily separated from a growth state and the cell characteristics can be preserved, and thus, the cells are recovered for experiment when needed. And a certain amount of cells are properly stored, so that the cells can be prevented from being lost due to pollution or other accidents of the cells being cultured, and the function of cell preservation is achieved.

Therefore, the immune cell cryopreservation cooling device is designed to solve the problems.

Disclosure of Invention

To solve the problems set forth in the background art described above. The invention provides an immune cell cryopreservation cooling device which is convenient for storing collected cells at low temperature and can improve the mixing efficiency of the cells in a test tube and a cryopreservation solution.

In order to achieve the purpose, the invention provides the following technical scheme: an immune cell cryopreservation cooling device comprises a base and a storage assembly arranged above the base;

deposit the subassembly including a jar body, deposit board, feed liquor pipe, liquid nitrogen chamber and drain pipe, the top of base is provided with the jar body, the internal board of depositing of installing of jar, a plurality of test-tube slots have been seted up on the surface of depositing the board, jar external lateral wall intercommunication has feed liquor pipe and drain pipe, just the feed liquor pipe with drain pipe one end extends to the liquid nitrogen intracavity that the internal portion of jar set up.

Preferably, the opening of the tank body is provided with a sealing cover, and the surface of the sealing cover is fixedly connected with a handle.

Preferably, the top end of the tank body is provided with an embedded groove, and a sealing ring is installed in the embedded groove.

Preferably, the outer side wall of the sealing cover is provided with a plurality of buckles, and the sealing cover is connected to the surface of the tank body through the buckles.

Preferably, the sealing cover is provided with a boss matched with the tank body on the surface of one side close to the tank body.

The device for freezing and storing immune cells and reducing the temperature of the immune cells is preferred to be further provided with a rotating assembly arranged on the surface of the base, the rotating assembly comprises a motor, a rotating shaft, a rotating seat, a hinge seat and hinge lugs, the motor is arranged on the bottom surface of the base, the rotating shaft of the motor penetrates through the surface of the base and is connected with one end of the rotating shaft arranged on the surface of the base, the rotating seat is fixedly connected to the other end of the rotating shaft, the hinge seat is fixedly arranged on the surface of the rotating seat, and the hinge seat is hinged to the hinge lugs arranged on the bottom surface of the tank body through shaft pins.

Preferably, the bottom surface of the swivel base is provided with a plurality of support rods in an annular distribution manner near the outer side of the rotating shaft, one end of each support rod is provided with a pulley, and the pulleys are arranged in annular grooves formed in the surface of the base in a sliding manner.

The immune cell cryopreservation cooling device preferably comprises a mixing assembly arranged on one side of a rotary seat, wherein the mixing assembly comprises a first bevel gear, a second bevel gear, a transmission rod, rotary frames, a sliding sleeve, a hinge rod, a hinge block, a sleeve and a butt screw rod, the two rotary frames are arranged on the bottom surface of the rotary seat, the surfaces of the same ends of the two rotary frames are rotatably connected with the transmission rod through bearings, the second bevel gear is arranged at one end of the transmission rod and meshed with the first bevel gear arranged on the outer side wall of the rotating shaft, the sleeve is slidably connected to the outer side wall of the other end of the transmission rod, the butt screw rod is in threaded connection with the outer side wall of the sleeve, one end of the butt screw rod penetrates through the surface of the sleeve and is in butt connection with a groove formed in the outer side wall of the transmission rod in an annular wavy shape, the outer side wall of the sleeve is hinged with one end of the hinge rod, the hinge block is hinged to the other end of the hinge rod, and the hinge block is fixedly arranged on the outer side wall of a tank body.

Preferably, a hinge part is fixed on the surface of the rotating seat, the hinge part is hinged with one end of a sleeve through a shaft pin, a sliding plug is connected in the sleeve in a sliding manner, one end, far away from the sleeve, of the sliding plug is connected with a hinge plate fixedly arranged on the outer side wall of the tank body through the shaft pin, a spring is arranged in the sleeve, one end of the spring abuts against the inside of the sleeve, and the other end of the spring abuts against one end of the sliding plug.

As the preferable immune cell freezing and cooling device, two ends of the hinged rod are both rounded.

Compared with the prior art, the invention has the beneficial effects that:

1. placing the test tube into a test tube groove, sealing the tank body by the sealing cover, injecting low-temperature liquid nitrogen into the liquid nitrogen cavity, cooling the test tube in the tank body by the liquid nitrogen, and preserving cells in the test tube at low temperature;

2. the rotating shaft and the rotary seat are driven to rotate by controlling the starting of the motor, the test tube in the tank body on the surface of the rotary seat is driven to rotate, the mixing efficiency of the cells and the frozen stock solution is accelerated by the rotation of the test tube, the cells and the frozen stock solution can be rapidly mixed, and the cells are prevented from breaking and dying in the cooling process;

3. make the sliding sleeve and drive the hinge bar and remove when the surface of transfer line slides, drive jar body and control the slope through the hinge lug and the articulated seat of bottom and rock on the swivel mount, the jar body under the cooperation running state of being convenient for further to the cell in the test tube and the mixing efficiency of freezing stock solution.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic view of the structure of the present invention;

FIG. 2 is a schematic bottom view of FIG. 1 according to the present invention;

FIG. 3 is a schematic view showing the inner structure of the sealing cap, the sealing ring and the can body according to the present invention;

FIG. 4 is a schematic structural view of a hinge seat, a swivel base, a support rod, a motor and a rotating shaft according to the present invention;

FIG. 5 is a schematic view of the construction of the transmission rod, the sliding sleeve, the hinge rod, the sleeve and the sliding plug of the present invention;

FIG. 6 is a schematic view of the structure of the transmission rod, the screw of the disk drive and the groove of the present invention;

FIG. 7 is a schematic diagram of the construction of the spring, sleeve and wiper plug of the present invention;

in the figure:

1. a base;

2. storing the assembly; 21. a tank body; 22. a storage plate; 23. a seal ring; 24. a sealing cover; 25. a boss; 26. buckling; 27. a liquid inlet pipe; 28. a liquid nitrogen cavity; 29. a liquid outlet pipe; 201. a test tube groove; 202. embedding a groove; 203. a handle;

3. a rotating assembly; 31. a motor; 32. a rotating shaft; 33. rotating; 34. a support bar; 35. a pulley; 36. an annular groove; 37. a hinged seat; 38. a hinge ear;

4. a mixing assembly; 41. a first bevel gear; 42. a second bevel gear; 43. a transmission rod; 45. a rotating frame; 46. a sliding sleeve; 47. a hinged lever; 48. a hinged block; 49. a hinge plate; 401. a sliding plug; 402. a sleeve; 403. a hinge portion; 404. a groove; 405. abutting against the screw; 407. a spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1;

an immune cell cryopreservation cooling device comprises a base 1.

In this embodiment: after the cells are collected and processed, the frozen stock solution needs to be added, the cells and the frozen stock solution are uniformly mixed and then are placed at the ultralow temperature for storage, and the cells are cooled from the room temperature to the ultralow temperature, and are required to be subjected to mixing processing in the process of slow cooling, so that the cells and the frozen stock solution are uniformly mixed and are cooled at the same time, the cells and the frozen stock solution are slowly mixed in the cooling process and are likely to cause cell death, the storage assembly 2 is added on the basis, and the collected cells are convenient to be stored at the low temperature through the assembly.

As shown in fig. 1, 2, 3, 4 and 5:

the above-mentioned content of set, in order to carry out low temperature with the cell after gathering and deposit, still including setting up the storage assembly 2 in base 1 top, storage assembly 2 includes jar body 21, storage plate 22, feed liquor pipe 27, liquid nitrogen chamber 28 and drain pipe 29, base 1's top is provided with jar body 21, install storage plate 22 in jar body 21, a plurality of test-tube slots 201 have been seted up on storage plate 22's surface, jar body 21 lateral wall intercommunication has feed liquor pipe 27 and drain pipe 29, and feed liquor pipe 27 and drain pipe 29 one end extend to in the inside liquid nitrogen chamber 28 that sets up of jar body 21.

In this embodiment: put into the test tube after gathering the cell and collect to put into the test tube with the test tube and deposit the test tube groove 201 on board 22 surface, let in microthermal liquid nitrogen through feed liquor pipe 27 in to liquid nitrogen chamber 28, cool down jar body 21 through the liquid nitrogen, carry out the cryopreservation to the cell in the test tube.

It should be noted that: the tank body 21 is made up of two tanks different in size, and a liquid nitrogen chamber 28 can be formed between the large tank and the small tank.

It should be noted that: valves are arranged on the surfaces of the liquid inlet pipe 27 and the liquid outlet pipe 29, the opening and closing of the liquid inlet pipe 27 and the liquid outlet pipe 29 are controlled through the valves, liquid nitrogen can be introduced into the liquid nitrogen cavity 28 through the liquid inlet pipe 27, and the liquid nitrogen can be discharged through the liquid outlet pipe 29.

In an alternative embodiment: a sealing cover 24 is arranged at the opening of the tank body 21, and a handle 203 is fixedly connected to the surface of the sealing cover 24.

In this embodiment: based on this setting to when putting into the test tube with the test tube and depositing the cell in the test tube and carry out the cryo-preservation in the test tube groove 201 on board 22 surface, the sealed lid 24 of accessible seals up jar body 21, ensures jar body 21 seal nature, conveniently takes off sealed lid 24 from jar body 21 through setting up accessible handle 203, increases the device flexibility.

In an alternative embodiment: the top end of the tank 21 is provided with an insertion groove 202, and a seal ring 23 is installed in the insertion groove 202.

In this embodiment: based on the arrangement, when the sealing cover 24 is used for sealing the tank 21, the sealing cover 24 can apply pressure to the sealing ring 23 on the surface of the tank 21, and the sealing effect between the sealing cover 24 and the tank 21 is improved.

In an alternative embodiment: the outer side wall of the sealing cover 24 is provided with a plurality of buckles 26, and the sealing cover 24 is connected to the surface of the can body 21 through the plurality of buckles 26.

In this embodiment: through this setting to when sealing lid 24 is sealed jar body 21, sealing lid 24 can be connected on the surface of jar body 21 through buckle 26, with the stability of improving the connection.

In an alternative embodiment: the sealing cover 24 is provided with a boss 25 matched with the can body 21 on one side surface close to the can body 21.

In this embodiment: based on the arrangement, when the sealing cover 24 is used for sealing the tank body 21, the boss 25 arranged on one side of the sealing cover 24 can be inserted into the tank body 21, so that the sealing cover 24 is prevented from deflecting during sealing, and the connection is convenient.

As shown in fig. 1, 2, 4 and 5:

combine above-mentioned content, in order to facilitate improving the cell in the test tube and freezing mixed efficiency of deposit liquid, still including installing the runner assembly 3 on base 1 surface, runner assembly 3 includes motor 31, pivot 32, swivel mount 33, articulated seat 37 and articulated ear 38, motor 31 is installed to the bottom surface of base 1, the surface of base 1 is run through to the axis of rotation of motor 31, and be connected with the one end that sets up at the pivot 32 on base 1 surface, the other end fixedly connected with swivel mount 33 of pivot 32, articulated seat 37 is installed to the fixed surface of swivel mount 33, articulated seat 37 is articulated mutually through the articulated ear 38 of the bottom surface installation of pivot and jar body 21.

In this embodiment: put into the test tube and deposit the test tube groove 201 on board 22 surface with the test tube, let in microthermal liquid nitrogen in to liquid nitrogen chamber 28 through feed liquor pipe 27, and with sealed lid 24 to jar body 21 after sealing, start through control motor 31, can drive pivot 32 and take place to rotate together with swivel mount 33, and then can make swivel mount 33's surface pass through articulated seat 37, the jar body 21 that pivot and articulated ear 38 are connected takes place to rotate, the test tube that drives and deposit board 22 surface test tube groove 201 rotates, the mixing efficiency of cell and frozen stock liquid is accelerated in rotation through the test tube, guarantee cell and frozen stock liquid can the flash mixed, prevent that the cell from breaking death at the cooling in-process.

In an alternative embodiment: a plurality of support rods 34 are annularly distributed on the outer side of the bottom surface of the rotating seat 33 close to the rotating shaft 32, pulleys 35 are mounted at one ends of the support rods 34, and the pulleys 35 are slidably arranged in annular grooves 36 formed in the surface of the base 1.

In this embodiment: based on this setting to in control motor 31 starts, can drive pivot 32 and take place to rotate together with pivot 32, when making jar body 21 take place to rotate, pulley 35 that bracing piece 34 one end of swivel mount 33's bottom surface installation was all installed can slide in the ring channel 36 that base 1 surface was seted up, supports swivel mount 33, improves pivoted stability, when preventing that jar body 21 from taking place to rotate, swivel mount 33 takes place to deflect, causes the dropping of jar body 21.

As shown in fig. 1, 2, 5, 6 and 7:

in combination with the above, in order to further improve the mixing efficiency of the cells and the frozen solution in the test tube, the mixing device further includes a mixing assembly 4 installed on one side of the swivel mount 33, the mixing assembly 4 includes a first bevel gear 41, a second bevel gear 42, a transmission rod 43, a rotating bracket 45, a sliding sleeve 46, a hinge rod 47, a hinge block 48, a sleeve 402 and a butt screw 405, two rotating brackets 45 are installed on the bottom surface of the swivel mount 33, the transmission rod 43 is rotatably connected to the same end surface of the two rotating brackets 45 through a bearing, the second bevel gear 42 is installed at one end of the transmission rod 43, the second bevel gear 42 is meshed with the first bevel gear 41 installed on the outer side wall of the rotating shaft 32, the sliding sleeve 46 is slidably connected to the outer side wall of the other end of the transmission rod 43, the butt screw 405 is threadedly connected to the outer side wall of the sliding sleeve 46, one end of the butt screw 405 penetrates through the surface of the sliding sleeve 46 and is butted in the groove 404 formed in the outer side wall of the transmission rod 43 in the shape of the annular wave, the outer side wall of the sliding sleeve 46 is hinged to one end of the hinge rod 47, the hinge block 48 is hinged to the hinge block 48, and the hinge block 48 is fixedly installed on the outer side wall of the tank 21.

In this embodiment: when the control motor 31 is started to drive the rotating shaft 32 and the rotating shaft 32 to rotate, so that the tank body 21 rotates, the first bevel gear 41 mounted on the rotating shaft 32 can drive the second bevel gear 42 to rotate, and further the transmission rod 43 connected with the second bevel gear 42 can rotate, at the same time, the sliding sleeve 46 slidably connected with one end of the transmission rod 43 can slide on the surface of the transmission rod 43, the abutting screw 405 is screwed into the threaded hole formed in the surface of the sliding sleeve 46, one end of the abutting screw 405 abuts against the groove 404 formed in the surface of one end of the transmission rod 43 (instead of abutting fixation, one end of the abutting screw 405 can slide in the groove 404), and when the transmission rod 43 rotates, the sliding sleeve 46 can stably move on the surface of the transmission rod 43 along the axial direction of the transmission rod 43 (namely, the circumferential rotation of the transmission rod 43 is converted into the reciprocating linear motion of the sliding sleeve 46), and when the sliding sleeve 46 slides on the surface of the transmission rod 43, the hinge 47 can be driven to move, so that the tank body 21 is driven to obliquely swing on the rotary base 33 left and right through the ear hinge 38 and the hinge 37 at the bottom end, so as to facilitate matching the tank body 21 in a rotating state, and further improve the mixing efficiency of cells in the frozen solution.

In an alternative embodiment: the surface of the rotating seat 33 is fixed with a hinge part 403, the hinge part 403 is hinged with one end of a sleeve 402 through a shaft pin, a sliding plug 401 is connected in the sleeve 402 in a sliding way, one end of the sliding plug 401, which is far away from the sleeve 402, is connected with a hinge plate 49 fixedly arranged on the outer side wall of the tank body 21 through the shaft pin, a spring 407 is arranged in the sleeve 402, one end of the spring 407 is abutted in the sleeve 402, and the other end of the spring 407 is abutted with one end of the sliding plug 401.

In this embodiment: based on this setting, so that the sliding sleeve 46 when the surface of transfer line 43 slides, can drive articulated rod 47 and remove, thereby it rocks to drive jar body 21 through the articulated ear 38 and the articulated seat 37 of bottom slope about on swivel mount 33, simultaneously jar body 21 is when the slope, can make sliding plug 401 slide in sleeve 402, exert pressure to the spring 407 in the sleeve 402, spring 407 compression deformation makes sliding plug 401 income sleeve 402 in, so that jar body 21 the vibrations that produce when rocking, sliding plug 401 through the setting of T shape structure can collude in sleeve 402 simultaneously, can carry on spacingly when jar body 21 inclines to the one side of keeping away from sleeve 402, prevent that jar body 21 from taking place to fall.

In an alternative embodiment: both ends of the hinge rod 47 are rounded.

In this embodiment: with this arrangement, it is avoided that a movement dead point exists between both ends of the hinge lever 47 and the slide sleeve 46 and the hinge block 48 when the slide sleeve 46 slides on the surface of the transmission lever 43.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides an immune cell cryopreserving heat sink, includes base (1), its characterized in that: the storage device also comprises a storage assembly (2) arranged above the base (1);

the storage assembly (2) comprises a tank body (21), a storage plate (22), a liquid inlet pipe (27), a liquid nitrogen cavity (28) and a liquid outlet pipe (29), the tank body (21) is arranged above the base (1), the storage plate (22) is installed in the tank body (21), a plurality of test tube grooves (201) are formed in the surface of the storage plate (22), the liquid inlet pipe (27) and the liquid outlet pipe (29) are communicated with the outer side wall of the tank body (21), and one end of each of the liquid inlet pipe (27) and the liquid outlet pipe (29) extends into the liquid nitrogen cavity (28) formed in the tank body (21);

the tank body is characterized by further comprising a rotating assembly (3) arranged on the surface of the base (1), wherein the rotating assembly (3) comprises a motor (31), a rotating shaft (32), a rotating seat (33), a hinging seat (37) and hinging lugs (38), the motor (31) is arranged on the bottom surface of the base (1), the rotating shaft of the motor (31) penetrates through the surface of the base (1) and is connected with one end of the rotating shaft (32) arranged on the surface of the base (1), the other end of the rotating shaft (32) is fixedly connected with the rotating seat (33), the hinging seat (37) is fixedly arranged on the surface of the rotating seat (33), and the hinging seat (37) is hinged with the hinging lugs (38) arranged on the bottom surface of the tank body (21) through shaft pins;

still including installing mixed subassembly (4) in swivel mount (33) one side, mixed subassembly (4) includes helical gear one (41), helical gear two (42), transfer line (43), rotating turret (45), sliding sleeve (46), articulated rod (47), articulated piece (48), sleeve (402) and butt screw rod (405), two are installed to the bottom surface of swivel mount (33) rotating turret (45), two same end surface of rotating turret (45) all is connected with through the bearing rotation transfer line (43), install the one end of transfer line (43) helical gear two (42), helical gear two (42) with install in the helical gear one (41) of pivot (32) lateral wall meshes mutually, the other end lateral wall sliding connection of transfer line (43) has sliding sleeve (46), the lateral wall threaded connection of sliding sleeve (46) has butt screw rod (405), the one end of butt screw rod (405) runs through the surface of sliding sleeve (46), and the butt in the recess (404) that the lateral wall of transfer line (43) was seted up in the annular wavy form, the lateral wall of sliding sleeve (46) with the one end of articulated rod (47), articulated rod (47) and articulated rod (47) is articulated mutually articulated, the hinge block (48) is fixedly arranged on the outer side wall of the tank body (21).

2. The immune cell cryopreservation and cooling device of claim 1, wherein: the opening of the tank body (21) is provided with a sealing cover (24), and the surface of the sealing cover (24) is fixedly connected with a handle (203).

3. The immune cell cryopreservation and cooling device of claim 1, wherein: the top end of the tank body (21) is provided with an embedded groove (202), and a sealing ring (23) is installed in the embedded groove (202).

4. The immune cell cryopreservation and cooling device of claim 2, wherein: a plurality of buckles (26) are installed on the outer side wall of the sealing cover (24), and the sealing cover (24) is connected to the surface of the tank body (21) through the buckles (26).

5. The immune cell cryopreservation and cooling device of claim 2, wherein: the sealing cover (24) is provided with a boss (25) matched with the tank body (21) on the surface of one side close to the tank body (21).

6. The immune cell cryopreservation and cooling device of claim 1, wherein: the bottom surface of swivel mount (33) is close to the outside of pivot (32) is that the annular distributes and installs a plurality of bracing pieces (34), and is a plurality of pulley (35) are all installed to the one end of bracing piece (34), pulley (35) slide to set up in annular groove (36) that base (1) surface was seted up.

7. The immune cell cryopreservation and cooling device of claim 1, wherein: the surface of the swivel base (33) is fixed with a hinge part (403), the hinge part (403) is hinged with one end of a sleeve (402) through a shaft pin, a sliding plug (401) is connected in the sleeve (402) in a sliding mode, one end, far away from the sleeve (402), of the sliding plug (401) is connected with a hinge plate (49) fixedly installed on the outer side wall of the tank body (21) through the shaft pin, a spring (407) is arranged in the sleeve (402), one end of the spring (407) abuts against the sleeve (402), and the other end of the spring (407) abuts against one end of the sliding plug (401).

8. The immune cell cryopreservation and cooling device of claim 1, wherein: the two ends of the hinge rod (47) are both rounded.