CN115369153B - Smad4 gene detection biosensor and preparation method thereof - Google Patents
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Abstract
The invention belongs to the technical field of biosensors, and relates to a Smad4 gene detection biosensor and a preparation method thereof. The Smad4 gene detection biosensor provided by the invention comprises a Smad4 gene detection DNA probe; the nucleotide sequence of the Smad4 gene detection DNA probe is as follows: 5' -SH- (CH) 2 ） 6 CATACCAGTCTAGACTTAT-3'. The invention combines electrochemistry, magnetic materials and nano molecules to detect the Smad4 sequence in the Smad protein pathway, and has excellent detection specificity, sensitivity, biocompatibility and detection limit performance.
Description
Technical Field
The invention belongs to the technical field of gene detection, and relates to a Smad4 gene detection biosensor and a preparation method thereof.
Background
The biosensor consists of a biomolecule recognition element and various physical and chemical transducers and is used for analyzing and detecting various living substances and chemical substances. The electrochemical DNA biosensor is an instrument which takes DNA as a recognition element and converts the concentration of the DNA into an electrochemical signal for detection. Electrochemical DNA biosensors can be divided into two categories: one type is an electrochemical biosensor based on DNA hybridization, which fixes single-stranded DNA on the surface of an electrode as a probe to realize the detection of probe complementary DNA. The other type is a non-gene-recognition electrochemical DNA sensor, which is a sensitive device with single-strand or double-strand DNA fixed on the surface of an electrode as a sensor, and realizes the detection and research of specific substances by using the action of other substances and the DNA or using the characteristics of the DNA. The electrochemical DNA hybridization biosensor is characterized in that SSDNA molecules are used as sensitive elements and fixed on the surface of an electrode, the SSDNA molecules are hybridized with target DNA by utilizing a molecular hybridization technology, and the sequence of the target DNA is determined by measuring the electrochemical signal change before and after the hybridization of an electroactive substance.
The nano gold refers to gold micro particles, the diameter of which is 1 to 100nm, the nano gold has high specific surface area and high surface energy, can be combined with various biological macromolecules, and does not influence the biological activity of the nano gold. The nano gold has the characteristics of common nano materials, namely surface effect, small-size effect, quantum size effect and tunnel quantum size effect, and meanwhile, the gold nanoparticles have unique optical properties and the capability of promoting electron transfer. Fe 3 O 4 Are one of the most important magnetic materials. Due to the multiple functions of the nano composite material, the biosensor can meet the requirements of quick response, high sensitivity and high selectivity. But still has the problems of complex preparation process, incapability of being reused, high cost and the like, and limits the clinical use of the medicine. CN113584129A provides a p53 gene detection probe, which consists of a porous hollow magnetic nanoparticle, an electrochemical redox active probe encapsulated inside the porous hollow magnetic nanoparticle, a cationic polymer functional layer coated on the surface of the porous hollow magnetic nanoparticle, and capture DNA adsorbed on the surface of the functional layer. However, the probe is packaged in a porous hollow magnetic nanoparticle mode, so that the preparation difficulty is high, and the success rate is low.
In mammals, 8 different Smad proteins, smad1 to Smad8, are found, and can be divided into 3 subfamilies: receptor-activated Smads (R-Smad), general-type Smads (Co-Smad), inhibitory Smads (I-Smad). Only one species of Co-Smad, smad4, is found in mammals. Binding of phosphorylated Smad3 to Smad4 may prevent binding of Smad4 to nuclear receptor chromosome maintenance region 1 (nuclear export receptor chromosome region 1) to localize Smad4 in the nucleus. Smad4 expression and mutations have significant effects on a variety of cancers, including colon cancer, and aberrant Smad4 expression (mutations or reduced numbers) correlates with the degree of tumor differentiation and Dukes staging. Therefore, the Smad4 gene detection biosensor is provided, detects the expression abnormality of Smad4, and has important significance for the related research of Smad protein channels.
Disclosure of Invention
The invention aims to develop a Smad4 gene detection biosensor.
In view of the above, the present invention provides a Smad4 gene detection biosensor and a method for preparing the same to satisfy the need in the art.
In one aspect, the present invention provides a Smad4 gene detection biosensor comprising a Smad4 gene detection DNA probe; the nucleotide sequence of the Smad4 gene detection DNA probe is as follows: 5' -SH- (CH) 2 ） 6 -CATACCAGTCTAGACTTAT-3’。
In another aspect, the present invention provides a method for preparing a Smad4 gene detection biosensor, comprising: dissolving the Smad4 gene detection DNA probe into a tris (2-carboxyethyl) phosphine solution to obtain an activated Smad4 gene detection DNA probe; hydroxyethyl piperazine ethyl sulfonicAcid and HAuCl 4 ·XH 2 Mixing O and pure water, heating and stirring to prepare a nano gold solution; and mixing the activated Smad4 gene detection DNA probe and the nano-gold solution at normal temperature, dripping the mixture on the surface of the treated gold electrode, reacting at 37 ℃ for 1.5h, immersing the mixture in mercaptoethanol, and sealing at 37 ℃ for 0.5h.
Further, in the preparation method of the Smad4 gene detection biosensor, the treatment method of the gold electrode comprises the following steps: method for preparing Fe by adopting in-situ chemical oxidative polymerization 3 O 4 Removing an oxide layer and impurities on the surface of the gold electrode by using a-PANI-AuNPs composite solution, immersing the gold electrode in an HDT ethanol solution, reacting for 1h at 4 ℃, sequentially cleaning the electrode by using absolute ethanol and ultrapure water, drying the electrode by using ultrapure nitrogen, and immersing the electrode in the Fe composite solution 3 O 4 And (3) placing the solution in the-PANI-AuNPs composite solution at 37 ℃ for 12h, washing with water and drying.
Furthermore, in the preparation method of the Smad4 gene detection biosensor, the Fe is calculated according to the quantitative ratio of substances 3 O 4 Fe in-PANI-AuNPs composite solution 3 O 4 The proportion of PANI to AuNPs is 1.
In another aspect, the present invention relates to a Smad4 gene detection method using the Smad4 gene detection biosensor described above.
Further, the Smad4 gene detection method provided by the invention comprises the following steps: the Smad4 gene detection biosensor was immersed in a sample solution to be detected, reacted at 37 ℃ for 1 hour, and then the surface of the electrode was washed with PBS buffer, 0.2% SDS, and ultrapure water in this order to remove non-hybridized target DNA, to obtain a modified gold electrode, which was subjected to electrochemical measurement.
Further, in the Smad4 gene detection method provided by the present invention, the electrochemical assay comprises: and stirring and immersing the modified gold electrode in 1mmol/L MB for 20min, then stirring in PBS and ultrapure water for 5min in sequence, and recording the DPV curve of the hybridized electrode in the PBS solution.
In another aspect, the present invention relates to the use of the Smad4 gene detection biosensor described above in Smad4 gene detection.
In another aspect, the present invention relates to the use of the Smad4 gene detection method described above for Smad4 gene detection.
Compared with the prior art, the invention has the following beneficial effects or advantages:
the invention provides a Smad4 gene detection biosensor and a preparation method thereof, on one hand, the invention designs a Smad4 gene detection DNA probe, which can realize the detection and identification of Smad4 gene in a Smad protein pathway; in another aspect, the invention provides a Smad4 gene detection biosensor, and Fe is designed 3 O 4 The PANI-AuNPs realize the combination of the probe and the electrode and can better detect the electric signal change of the Smad protein channel in the sample to be detected.
Drawings
FIG. 1 shows the peak current values after hybridization of probe sequences with target sequences at different concentrations.
FIG. 2 shows the peak current change after hybridization of the probe sequence with different sequences at the same concentration.
Detailed Description
In order to make the technical solutions of the present invention better understood and implemented by those skilled in the art, the present invention is further described below with reference to the following specific embodiments and the accompanying drawings, but the embodiments are not meant to limit the present invention.
The experimental methods and the detection methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.
Example 1
This example provides the preparation of a Smad4 gene detection biosensor.
And (3) probe:
chemically synthesizing a Smad4 gene detection DNA probe; the nucleotide sequence of the Smad4 gene detection DNA probe is as follows: 5' -SH- (CH) 2 ） 6 -CATACCAGTCTAGACTTAT-3’。
Fe 3 O 4 -PANI-AuNPs complex solution preparation:
the Fe is calculated according to the mass ratio 3 O 4 Fe in-PANI-AuNPs composite solution 3 O 4 The proportion of PANI to AuNPs is 1.
50mL of a 0.01% strength by mass/volume tetrachloroauric acid solution are brought to boiling with vigorous stirring. Then 2mL of trisodium citrate at 1% by mass/volume are added rapidly to the boiling solution. When the color of the solution is changed from light yellow to wine red, the AuNPs solution is prepared.
1.25g of dodecylbenzenesulfonic acid (DBSA) and 0.5mL of aniline monomer were dissolved in 50mL of distilled water, and after stirring for 20min, 25mL of an aqueous solution containing 1.25g of (NH) S20S was added dropwise, and the reaction was carried out at a constant temperature for 8 hours. After the reaction is completed, CHC1 is used 3 Extracting to obtain PANI (DBSA)/CHC 1 3 And (3) solution. Mixing the solution with a certain amount of FeCl 3 ·6H 2 O and FeC1 2 ·4H 2 O solution (FeCl) 3 ·6H 2 O and FeC1 2 ·4H 2 The mass ratio of O is 2: 1) And uniformly mixing, heating under reflux and stirring for reaction, adding a certain amount of uncooled AuNPs solution, uniformly and slowly dripping the heated NaOH solution after 30min until the pH value is =7.4, and continuously reacting for 8h. After the reaction was completed, CHC1 was used again 3 Extracting, washing with distilled water to obtain the Fe 3 O 4 -PANI-AuNPs complex solution.
And (3) treating the treated gold electrode:
polishing aluminum oxide powder to remove an oxide layer on the surface of the gold electrode, removing impurities on the surface of the gold electrode by ultrasonic, immersing the gold electrode into 2.5mmol/L HDT ethanol solution, reacting for 1h at 4 ℃, cleaning the electrode by absolute ethyl alcohol and ultrapure water in sequence, and drying by blowing with ultrapure nitrogen. Immersing in 1mol/L Fe 3 O 4 And (3) placing the mixture in the PANI-AuNPs compound solution for 12 hours at 37 ℃, washing with water and drying in the air.
Preparation of Smad4 gene detection biosensor:
and dissolving the Smad4 gene detection DNA probe into a tris (2-carboxyethyl) phosphine solution to obtain 50 mu L of 3 mu mol/L activated Smad4 gene detection DNA probe. Mixing hydroxyethyl piperazine ethyl sulfonic acid and HAuCl 4 ·XH 2 And mixing O and pure water, heating and stirring to prepare the nano gold solution. 50. Mu.L of 3. Mu. Mol/L activated Smad4 gene was examinedMixing the DNA detection probe and 50mL of nano-gold solution of 5mmol/L at normal temperature, dripping the mixture on the surface of the treated gold electrode, reacting at 37 ℃ for 1.5h, immersing the mixture in mercaptoethanol of 1mmol/L, and sealing at 37 ℃ for 0.5h.
Example 2
This example provides detection by a Smad4 gene detection biosensor.
Target DNA sequence: 5'-ATAAGTCTAGACTGGTATG-3';
single base mismatches: 5'-ATAACTCTAGACTGGTATG-3';
non-complementary sequences: 5'-GCGGTCTCGAGTCAACACA-3'.
Adding TE buffer solution with pH 7.4 in the amount required by the preparation concentration into each sequence DNA, then slightly covering the tube cover, shaking uniformly, and preparing into 1.0 × 10 5 And (5) freezing and storing the solution in mol/L.
The assembled Smad4 gene detection biosensor was immersed in 100. Mu.L of target DNA solutions of different concentrations, reacted at 37 ℃ for 1 hour, and then the surface of the gold electrode was washed with PBS buffer, 0.2% SDS, and ultrapure water in this order to remove non-hybridized target DNA, i.e., hybridized gold electrode, and then subjected to electrochemical measurement.
The test adopts a three-electrode system, and takes a hybridized gold electrode as a working electrode, a saturated calomel electrode as a reference electrode and a platinum electrode as a counter electrode. In the presence of 1mmol/L [ Fe (CN) ] containing 0.1mol/L KCl 6 ] 3-/4- In the solution, an electrochemical workstation records the electrochemical impedance spectrum of each modified gold electrode, and the frequency range is 10 5 Hz-0.01Hz。
The Smad4 gene detection biosensor was immersed in 1mmol/L MB under stirring for 20min, and then stirred in PBS and ultrapure water for 5min in order to remove non-specifically adsorbed MB, and the DPV curve of the post-hybridization electrode was recorded in the PBS solution. The PBS buffer was flushed with nitrogen for 20min prior to the assay to remove oxygen.
FIG. 1 is a regression curve of MB after hybridization of probe sequences with different concentrations of target sequences. The peak current variation (Delta I) and the logarithm of DNA concentration (lgc) DNA ) In a very good linear relationship, the regression equation Δ I =0.7642 lgc DNA +11.084, linear correlationThe coefficient is 0.9992, the detection limit is 1.3 × 10 -13 mol/L（σ=3）。
To further verify the selectivity of the biosensor prepared according to the present invention, 1X 10 was used -5 Change of detection signals of different DNA sequences of target DNA sequence (TD), single base mismatch target (SM) and non-complementary DNA (NC) in mol/L. Smad4 gene detection biosensor at 1mmol/L [ Ru (NH) ] 3 ) 6 ] 3+ After immersing for 20min under stirring, stirring in PBS and ultrapure water for 5min to remove non-specifically adsorbed [ Ru (NH) 3 ) 6 ] 3+ The DPV curves of the hybridized electrodes were recorded in PBS solution. The PBS buffer was flushed with nitrogen for 20min prior to the assay to remove oxygen.
As can be seen from FIG. 2, the electrochemical signal of the non-complementary DNA is very low for different DNA sequences at the same concentration as the target, and the electrochemical signal of the single base mismatch target (SM) is lower than that of the target DNA sequence (TD) for the single base mismatch target DNA sequence at the same concentration as the target DNA sequence. As can be seen from comparison with FIG. 1, [ Ru (NH) is used 3 ) 6 ] 3+ As an indicator, it is less effective than MB. The result shows that the biosensor constructed by the strategy of the invention has good selectivity and discrimination capability of base mutation analysis on the target DNA, and has huge application potential.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (7)
1. A Smad4 gene detection biosensor comprising a Smad4 gene detection DNA probe; the nucleotide sequence of the Smad4 gene detection DNA probe is as follows: 5' -SH- (CH) 2 ） 6 -CATACCAGTCTAGACTTAT-3’；
The preparation method of the Smad4 gene detection biosensor comprises the following steps: the Smad4 groupThe detection DNA probe is dissolved in the tris (2-carboxyethyl) phosphine solution to obtain an activated Smad4 gene detection DNA probe; mixing hydroxyethyl piperazine ethyl sulfonic acid and HAuCl 4 ·XH 2 Mixing O and pure water, heating and stirring to prepare a nano gold solution; mixing the activated Smad4 gene detection DNA probe and the nano-gold solution at normal temperature, dripping the mixture on the surface of a treated gold electrode, reacting at 37 ℃ for 1.5h, immersing the mixture in mercaptoethanol, and sealing at 37 ℃ for 0.5h;
the processing method of the gold electrode comprises the following steps: method for preparing Fe by adopting in-situ chemical oxidative polymerization 3 O 4 Removing an oxide layer and impurities on the surface of the gold electrode by using a-PANI-AuNPs composite solution, immersing the gold electrode in an HDT ethanol solution, reacting for 1h at 4 ℃, sequentially cleaning the electrode by using absolute ethanol and ultrapure water, drying the electrode by using ultrapure nitrogen, and immersing the electrode in the Fe composite solution 3 O 4 Placing the mixture in a-PANI-AuNPs composite solution for 12 hours at 37 ℃, washing with water and drying in the air; the Fe is calculated by the amount ratio of the substances 3 O 4 Fe in-PANI-AuNPs composite solution 3 O 4 The proportion of PANI to AuNPs is 1;
when the Smad4 gene detection biosensor detects the Smad4 gene, the Smad4 gene detection biosensor is matched with MB for use.
2. A method for gene detection of Smad4 for non-diagnostic purposes, which comprises using the Smad4 gene detection biosensor according to claim 1.
3. The method of detecting a Smad4 gene according to claim 2, comprising: the Smad4 gene detection biosensor was immersed in a sample solution to be detected, reacted at 37 ℃ for 1 hour, and then the surface of the electrode was washed with PBS buffer, 0.2% SDS, and ultrapure water in this order to remove non-hybridized target DNA, to obtain a modified gold electrode, which was subjected to electrochemical measurement.
4. The Smad4 gene detection method of claim 2, wherein the electrochemical assay comprises: the modified gold electrode is stirred and immersed in MB with the concentration of 1mmol/L for 20min, then stirred in PBS and ultrapure water for 5min in sequence, and the DPV curve of the hybridized electrode is recorded in the PBS solution.
5. Use of the Smad4 gene detection biosensor of claim 1 for the non-diagnostic purpose detection of the Smad4 gene.
6. Use of the detection method using the Smad4 gene detection biosensor according to claim 2 in the detection of Smad4 gene for non-diagnostic purposes.
7. The use of claim 6, wherein said detection of Smad4 gene for non-diagnostic purposes comprises detection of a sample comprising a human Smad4 gene.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942386A (en) * | 2009-07-08 | 2011-01-12 | 中国科学院广州生物医药与健康研究院 | Nucleic acid nanogold biosensor and preparation method thereof |
| CN104849446A (en) * | 2015-01-07 | 2015-08-19 | 青岛科技大学 | Preparation method for biosensor used for detecting P53 protein based on nanoparticle amplification technology |
| CN113584129A (en) * | 2021-07-30 | 2021-11-02 | 青岛科技大学 | P53 gene detection probe, obtained biosensor and application thereof |
| CN115165807A (en) * | 2022-06-28 | 2022-10-11 | 江苏科技大学 | FOLSPR aptamer sensor based on AuNPs polymer and preparation method and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002363062B2 (en) * | 2001-10-09 | 2007-03-22 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| US10203325B2 (en) * | 2011-11-09 | 2019-02-12 | Board Of Trustees Of Michigan State University | Metallic nanoparticle synthesis with carbohydrate capping agent |
| CN112697858B (en) * | 2020-12-08 | 2022-02-18 | 云南民族大学 | Electrochemical analysis method for determining activity of T4 polynucleotide kinase based on magnetic nano material |
| CN114657185B (en) * | 2022-03-28 | 2023-11-10 | 福州大学 | Gold magnetic nano probe based on ordered arrangement of aptamer and application of gold magnetic nano probe in field halichondrin acid detection |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942386A (en) * | 2009-07-08 | 2011-01-12 | 中国科学院广州生物医药与健康研究院 | Nucleic acid nanogold biosensor and preparation method thereof |
| CN104849446A (en) * | 2015-01-07 | 2015-08-19 | 青岛科技大学 | Preparation method for biosensor used for detecting P53 protein based on nanoparticle amplification technology |
| CN113584129A (en) * | 2021-07-30 | 2021-11-02 | 青岛科技大学 | P53 gene detection probe, obtained biosensor and application thereof |
| CN115165807A (en) * | 2022-06-28 | 2022-10-11 | 江苏科技大学 | FOLSPR aptamer sensor based on AuNPs polymer and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| "Electrochemical Immunosensor Based on Fe3O4/PANI/AuNP Detecting Interface for Carcinoembryonic Antigen Biomarker";CHAMALI AMARASIRI等;《Journal of ELECTRONIC MATERIALS》;20170617;第46卷(第10期);参见说明书[0005]-[0038]、[0049]-[0056]段 * |
| 纳米金探针在基因检测中的应用;周政;《生命科学》;20090615(第03期);全文 * |
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