CN115362265A - 测量样品的pH的方法 - Google Patents

测量样品的pH的方法 Download PDF

Info

Publication number
CN115362265A
CN115362265A CN202180026611.1A CN202180026611A CN115362265A CN 115362265 A CN115362265 A CN 115362265A CN 202180026611 A CN202180026611 A CN 202180026611A CN 115362265 A CN115362265 A CN 115362265A
Authority
CN
China
Prior art keywords
electrochemical cell
solution
electrode
sample
electrochemical
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180026611.1A
Other languages
English (en)
Chinese (zh)
Inventor
K·普尼亚尼
P·A·尼贝里
S·索斯塔德
M·皮科克
L·熊
J·Y·苏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Degner Biotech
Original Assignee
Degner Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degner Biotech filed Critical Degner Biotech
Publication of CN115362265A publication Critical patent/CN115362265A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/302—Electrodes, e.g. test electrodes; Half-cells pH sensitive, e.g. quinhydron, antimony or hydrogen electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G01N21/80—Indicating pH value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/4163—Systems checking the operation of, or calibrating, the measuring apparatus
    • G01N27/4165—Systems checking the operation of, or calibrating, the measuring apparatus for pH meters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/4166—Systems measuring a particular property of an electrolyte
    • G01N27/4167—Systems measuring a particular property of an electrolyte pH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/115—Characterised by chemical treatment oxidising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/119—Reactions demanding special reaction conditions pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Plasma & Fusion (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180026611.1A 2020-04-03 2021-04-06 测量样品的pH的方法 Pending CN115362265A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP20168019 2020-04-03
EP20168019.6 2020-04-03
EP20197572.9 2020-09-22
EP20197572 2020-09-22
PCT/EP2021/058946 WO2021198533A1 (en) 2020-04-03 2021-04-06 A method of measuring the ph of a sample

Publications (1)

Publication Number Publication Date
CN115362265A true CN115362265A (zh) 2022-11-18

Family

ID=75339765

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180026611.1A Pending CN115362265A (zh) 2020-04-03 2021-04-06 测量样品的pH的方法

Country Status (14)

Country Link
US (1) US20230074766A1 (he)
EP (1) EP3987059B1 (he)
JP (1) JP2023520419A (he)
KR (1) KR20220161286A (he)
CN (1) CN115362265A (he)
AU (1) AU2021249488A1 (he)
BR (1) BR112022017446A2 (he)
CA (1) CA3168685C (he)
DK (1) DK3987059T3 (he)
ES (1) ES2928304T3 (he)
IL (1) IL296011B2 (he)
MX (1) MX2022012139A (he)
WO (1) WO2021198533A1 (he)
ZA (1) ZA202208804B (he)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117147662A (zh) * 2023-08-02 2023-12-01 中南林业科技大学 一种大肠杆菌活性的电化学检测方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2932875A1 (es) * 2021-07-20 2023-01-27 Univ Oviedo Método para la detección de un material genético en una muestra y dispositivo para llevarlo a cabo
WO2024064704A1 (en) * 2022-09-19 2024-03-28 Rt Microdx Inc. Pathogen testing device and method
CN116908244B (zh) * 2023-09-13 2023-12-22 四川省林业和草原调查规划院 一种林业生态保护用采样装置

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4820399A (en) * 1984-08-31 1989-04-11 Shimadzu Corporation Enzyme electrodes
JP2004141158A (ja) * 2002-10-01 2004-05-20 Matsushita Electric Ind Co Ltd プライマーの伸長反応検出方法、塩基種判別方法、塩基種判別装置、ピロリン酸の検出装置、核酸の検出方法および試料溶液導入チップ
US20160084863A1 (en) * 2011-09-25 2016-03-24 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) * 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
GB2501769A (en) * 2012-05-04 2013-11-06 Schlumberger Holdings Electrochemical sensor for pH measurement
US9034606B2 (en) 2012-08-23 2015-05-19 New England Biolabs, Inc. Detection of an amplification reaction product using pH-sensitive dyes
CN104232615A (zh) * 2013-06-09 2014-12-24 中国科学院上海应用物理研究所 基于电化学-dna反应控制芯片的酸碱法扩增dna片段技术
US10011549B2 (en) * 2015-07-06 2018-07-03 Robert Bosch Gmbh Electrochemically active agents for pH modulation in biological buffers
WO2017155858A1 (en) * 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US11162133B2 (en) 2016-06-03 2021-11-02 New England Biolabs, Inc. Detection of an amplification product using pH-sensitive dye

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117147662A (zh) * 2023-08-02 2023-12-01 中南林业科技大学 一种大肠杆菌活性的电化学检测方法
CN117147662B (zh) * 2023-08-02 2024-03-08 中南林业科技大学 一种大肠杆菌活性的电化学检测方法

Also Published As

Publication number Publication date
BR112022017446A2 (pt) 2023-01-17
IL296011B2 (he) 2023-07-01
ES2928304T3 (es) 2022-11-16
JP2023520419A (ja) 2023-05-17
CA3168685A1 (en) 2021-10-07
DK3987059T3 (da) 2022-10-03
US20230074766A1 (en) 2023-03-09
WO2021198533A1 (en) 2021-10-07
KR20220161286A (ko) 2022-12-06
EP3987059A1 (en) 2022-04-27
ZA202208804B (en) 2023-04-26
MX2022012139A (es) 2022-10-18
AU2021249488A1 (en) 2022-09-08
CA3168685C (en) 2023-05-16
IL296011A (he) 2022-10-01
IL296011B1 (he) 2023-03-01
EP3987059B1 (en) 2022-07-06

Similar Documents

Publication Publication Date Title
CA3168685C (en) A method of measuring the ph of a sample
Won et al. Investigation of the signaling mechanism and verification of the performance of an electrochemical real-time PCR system based on the interaction of methylene blue with DNA
US20200362404A1 (en) Electrochemical Detection Of Polymerase Reactions By Specific Metal-Phosphate Complex Formation
DK2373805T3 (en) Reagent LOW TOTAL SALT AND SYSTEMS FOR biosensors
WO2007083693A1 (ja) ピロリン酸センサおよびそれを用いたｓｎｐタイピングセンサ
EP1953541B1 (en) Method for determination of amount of double-stranded dna
KR101345077B1 (ko) 표적 핵산 서열의 전기화학적 검출방법
JP4765813B2 (ja) 二重鎖ｄｎａ量の電気化学的測定方法
WO2010030035A1 (en) Impedance spectroscopy measurement of dna
JPWO2005003750A1 (ja) ピロリン酸検出センサ、核酸の検出方法、および塩基種判別方法
JP3866277B2 (ja) ピロリン酸測定方法及びプライマー伸張反応検出方法、並びにこれら方法を実施するための装置
JP4908182B2 (ja) 核酸増幅の有無の判定方法、標的核酸の検出方法及びそれらに用いられる装置
Dong Development of Field-deployable Nucleic Acid Testing Platforms
RU114320U1 (ru) УСТРОЙСТВО ДЛЯ ПРЯМОЙ ЭЛЕКТРОХИМИЧЕСКОЙ РЕГИСТРАЦИИ НЕПРАЙМИРОВАННОГО СИНТЕЗА МОЛЕКУЛ ДНК НА ОСНОВЕ pН-ЧУВСТВИТЕЛЬНОГО СЕНСОРА
Luo et al. Functional Nucleic Acid Based Platforms for Heavy Metal Ion Detection
WO2023178277A1 (en) Closed bipolar electrode based biosensors
JP2006145342A (ja) ポリヌクレオチドの分析方法
JP2007037483A (ja) 酵素反応の有無の検出方法
GRIMALDI DEVELOPMENT OF INNOVATIVE TECHNOLOGIES FOR DNAEXTRACTION AND DETECTION
JP2010266313A (ja) 二本鎖ｄｎａの検出方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination