CN115316589A - Method for removing agglutinin in stem and leaf of pseudo-ginseng - Google Patents
Method for removing agglutinin in stem and leaf of pseudo-ginseng Download PDFInfo
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- CN115316589A CN115316589A CN202110342649.1A CN202110342649A CN115316589A CN 115316589 A CN115316589 A CN 115316589A CN 202110342649 A CN202110342649 A CN 202110342649A CN 115316589 A CN115316589 A CN 115316589A
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- notoginseng
- agglutinin
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- 101710186708 Agglutinin Proteins 0.000 title claims abstract description 18
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- 101710150763 Mannose-specific lectin 1 Proteins 0.000 title claims abstract description 18
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 title claims abstract description 18
- 244000131316 Panax pseudoginseng Species 0.000 title claims abstract description 15
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- 238000000034 method Methods 0.000 title claims abstract description 11
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/276—Treatment with inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a method for removing agglutinin in stem and leaf of notoginseng, which comprises the steps of washing fresh stem and leaf of notoginseng with clear water, and cutting into segments; then putting the pseudo-ginseng stem leaves into a packaging bag containing a mixed solution of L-ascorbic acid and sodium sulfite, immersing pseudo-ginseng stem leaves in the liquid, and then carrying out sealed packaging; placing the packaged stem and leaf of Panax notoginseng in an ultrahigh pressure container for ultrahigh pressure treatment; and filtering the liquid in the packaging bag subjected to the ultrahigh pressure treatment, and then entering the next procedure. The invention treats the stem and leaf of panax notoginseng with L-ascorbic acid and sodium sulfite under the action of ultra-high pressure, which destroys the anti-nutritional factor-agglutinin in the stem and leaf of panax notoginseng, and simultaneously keeps the form and nutritional ingredients of the stem and leaf of panax notoginseng better, thereby laying a good foundation for direct eating or subsequent processing.
Description
Technical Field
The invention relates to a method for removing agglutinin in stems and leaves of panax notoginseng, belonging to the field of food nutrition.
Background
The stem and leaf of Panax notoginseng contain high protein, crude fiber, vitamin C, carotene, low fat content, and abundant mineral elements (zinc, iron, and manganese content). The stem and leaf of Panax notoginseng contains about 4-6% of total saponins, mainly protopanaxadiol type saponins, wherein ginsenoside Rb is ginsenoside 3 Ginsenoside Rc, notoginsenoside Fc and ginsenoside Rb 1 The content is higher. In addition, there are flavone, flavonoid glycoside, saccharide, terpenoid, etc.
In Ben Cao gang mu, it is said that the stem and leaf of Panax notoginseng are good at stopping bleeding and relieving swelling, because it can be used for treating fracture and bleeding, and its swelling can be relieved after application. In ancient times, people use fresh stem leaves of radix Notoginseng to treat scald, and chew and swallow fresh stem leaves of radix Notoginseng to treat diseases such as gingival swelling and pain, oral mucosa ulcer and chronic pharyngitis. Modern pharmacological research finds that the arasaponin has sedative hypnotic and analgesic effects on the central nervous system and has the effect of reducing blood fat in the aspects of cardiovascular and cerebrovascular diseases. In addition, the anti-inflammatory action of the stem and leaf of panax notoginseng is probably related to the fact that the stem and leaf of panax notoginseng contains panaxadiol glycoside. Notoginseng radix saponin can delay aging.
However, the stem and leaves of panax notoginseng contain much anti-nutritional factor, namely agglutinin, which was first discovered by Stillmark in 1889 and is a factor inhibiting the digestion and utilization of protein, and has been called hemagglutinin for a long time. The agglutinin is a protein, has strong binding capacity and a three-dimensional structure in the reaction process of glycoprotein or carbohydrate, has different contents of different plant agglutinin, can interfere the secretion function of enzyme, influence the absorption of protein, and has the functions of hindering and stopping the growth of a living body. So far, no report of the agglutinin treatment method in the stem and leaf of panax notoginseng exists in the prior art.
Although the edible and medicinal values of the stem and leaf of panax notoginseng are proved by folks, if the anti-nutritional factor, namely agglutinin, is not removed, the digestion and absorption of the human body to protein are influenced. The invention uses L-ascorbic acid and sodium sulfite to cooperate with ultrahigh pressure to treat the stem leaves of the panax notoginseng, can well passivate the activity of agglutinin in the stem leaves, improve the edible value of the stem leaves, and lay a good foundation for direct eating or subsequent processing.
Disclosure of Invention
The invention aims to provide a method for removing antinutritional factors-agglutinin in from stems and leaves of panax notoginseng, which comprises the steps of washing fresh stems and leaves of panax notoginseng with clear water, draining water and cutting into small sections of 1.5 cm; then putting the pseudo-ginseng leaves into a packaging bag containing 0.2 to 0.4 mass percent of L-ascorbic acid and 0.004 to 0.008 mass percent of sodium sulfite aqueous solution, preferably just immersing the pseudo-ginseng stems and leaves in liquid, and then sealing and packaging; the well packaged stem leaves of the pseudo-ginseng are put under 400 to 600MPa for 10 to 25 minutes of ultrahigh pressure treatment; then opening the bag to filter the liquid.
The method comprises the following specific operation steps:
(1) Selecting materials, cleaning and cutting: selecting fresh stem and leaf of Notoginseng radix, washing with flowing clear water, draining, and cutting into 1.5cm segments;
(2) Preparing a compound liquid: the composition of the complex liquid comprises 0.2 to 0.4 mass percent of L-ascorbic acid, 0.004 to 0.008 mass percent of sodium sulfite and the balance of water. The purpose is as follows: inactivating the activity of agglutinin in the stems and leaves of the panax notoginseng by cooperating with ultrahigh pressure treatment;
(3) Bagging and packaging: placing Notoginseng radix stem and leaf cleaned with flowing clear water and cut into segments into packaging bag, injecting compound liquid until Notoginseng radix stem and leaf is just immersed in compound liquid, and sealing and packaging;
(4) Ultrahigh pressure treatment: and (3) placing the hermetically packaged stem leaves of the pseudo-ginseng under the pressure of 400-600 MPa for ultrahigh pressure treatment for 10-25 minutes, and then opening the bag to filter out liquid.
The invention has the beneficial effects that: the L-ascorbic acid and sodium sulfite cooperate with ultrahigh pressure treatment on the stem and leaf of the panax notoginseng, so that the activity of agglutinin in the panax notoginseng can be well passivated, the edible value of the panax notoginseng can be improved, and a good foundation is laid for direct eating or subsequent processing of the panax notoginseng.
Detailed Description
The present invention is further illustrated by the following specific examples, but the scope of the present invention is not limited thereto, and the method in this example is performed by a conventional method unless otherwise specified, and the reagents used are conventional reagents or reagents prepared by a conventional method unless otherwise specified.
Example 1
(1) Selecting materials, cleaning and cutting: selecting fresh stem leaves of Panax notoginseng, washing with flowing clear water, draining, and cutting into 1.5cm segments;
(2) Preparing a compound liquid: the composite liquid comprises 0.2 percent of L-ascorbic acid, 0.008 percent of sodium sulfite and the balance of water by mass percent. The purpose is as follows: the activity of agglutinin in the notoginseng flower is passivated by cooperating with ultrahigh pressure treatment;
(3) Bagging and packaging: placing Notoginseng radix stem and leaf cleaned with flowing clear water and cut into segments into packaging bag, injecting compound liquid until Notoginseng radix stem and leaf are just immersed in compound liquid, and sealing and packaging;
(4) Ultrahigh pressure treatment: and (3) placing the hermetically packaged stem leaves of the pseudo-ginseng under 400MPa for ultrahigh pressure treatment for 25 minutes, and then opening the bag to filter out liquid.
Example 2
(1) Selecting materials, cleaning and cutting: selecting fresh stem and leaf of Notoginseng radix, washing with flowing clear water, draining, and cutting into 1.5cm segments;
(2) Preparing a compound liquid: the composition of the complex liquid comprises 0.4 percent of L-ascorbic acid, 0.004 percent of sodium sulfite and the balance of water by mass percent. The purpose is as follows: the activity of agglutinin in the notoginseng flower is passivated by cooperating with ultrahigh pressure treatment;
(3) Bagging and packaging: loading Notoginseng radix stem and leaf cleaned with flowing clear water and cut into segments into packaging bag, injecting composite liquid, and sealing and packaging;
(4) Ultrahigh pressure treatment: and (3) placing the sealed and packaged stem leaves of the pseudo-ginseng under 600MPa for carrying out ultrahigh pressure treatment for 10 minutes, and then opening the bag to filter out liquid to obtain the pseudo-ginseng stem leaf tea.
Example 3
(1) Selecting materials, cleaning and cutting: selecting fresh stem and leaf of Notoginseng radix, washing with flowing clear water, draining, and cutting into 1.5cm segments;
(2) Preparing a compound liquid: the composite liquid comprises 0.3 percent of L-ascorbic acid, 0.006 percent of sodium sulfite and the balance of water by mass percent. The purpose is as follows: inactivating the activity of agglutinin in Notoginseng flower by synergistic ultrahigh pressure treatment;
(3) Bagging and packaging: placing Notoginseng radix stem and leaf cleaned with flowing clear water and cut into segments into packaging bag, injecting compound liquid until Notoginseng radix stem and leaf are just immersed in compound liquid, and sealing and packaging;
(4) Ultrahigh pressure treatment: and (3) placing the hermetically packaged stem leaves of the pseudo-ginseng under 500MPa for ultrahigh pressure treatment for 18 minutes, and then opening the bag to filter out liquid.
The lectin activity in the stems and leaves of panax notoginseng before and after treatment is analyzed and detected, and the result is shown in table 1:
table 1: comparison of Agglutinin Activity in Stem and leaf of Panax notoginseng before and after treatment
Note: lectin activity assay reference: a M Culderon Dela Barca, L Vazzez-Moreno, M R Robles-Burgunueno. Active sobean leins in foods, isolation and qualification. FoodChemisty, 1991, 39.
The results show that: the treatment technology can make the activity of agglutinin in Notoginseng radix stem and leaf be zero.
Claims (2)
1. A method for removing agglutinin in stem and leaf of Panax notoginseng is characterized by comprising the following steps: washing fresh stem and leaf of Notoginseng radix with clear water, draining, and cutting; then putting the pseudo-ginseng stem leaves into a packaging bag containing 0.2-0.4 mass percent of L-ascorbic acid and 0.004-0.008 mass percent of sodium sulfite aqueous solution, immersing pseudo-ginseng stem leaves in the liquid, and then sealing and packaging; and (3) placing the packaged stem leaves of the pseudo-ginseng under 400-600 MPa for ultrahigh pressure treatment for 10-25 minutes, and then opening the bag to filter out liquid.
2. The method for removing the agglutinin from the stems and leaves of panax notoginseng according to claim 1, which comprises the following steps:
(1) Selecting materials, cleaning and cutting: selecting fresh stem leaves of Panax notoginseng, washing with flowing clear water, draining, and cutting into 1.5cm segments;
(2) Preparing a compound liquid: the composition of the complex liquid comprises 0.2 to 0.4 mass percent of L-ascorbic acid, 0.004 to 0.008 mass percent of sodium sulfite and the balance of water;
the purpose is as follows: inactivating the activity of anti-nutritional factor-agglutinin in the stem and leaf of Panax notoginseng by cooperating with ultrahigh pressure treatment;
(3) Bagging and packaging: loading Notoginseng radix stem and leaf cleaned with flowing clear water and cut into a packaging bag, injecting compound liquid to immerse Notoginseng radix stem and leaf, and sealing and packaging;
(4) Ultrahigh pressure treatment: and (3) carrying out ultrahigh pressure treatment on the sealed and packaged stem leaves of the pseudo-ginseng for 10-25 minutes under 400-600 MPa, and then opening the bag to filter out the liquid.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003040319A2 (en) * | 2001-11-02 | 2003-05-15 | Wisconsin Alumni Research Foundation | Endothelial cells derived from primate embryonic stem cells |
| US20130303563A1 (en) * | 2011-11-10 | 2013-11-14 | Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center | Treatment of peritoneal injury using jak inhibitors |
| KR20160117850A (en) * | 2015-03-31 | 2016-10-11 | 중앙대학교 산학협력단 | Method for preparing lectin with reduced blood coagulation activity |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003040319A2 (en) * | 2001-11-02 | 2003-05-15 | Wisconsin Alumni Research Foundation | Endothelial cells derived from primate embryonic stem cells |
| US20130303563A1 (en) * | 2011-11-10 | 2013-11-14 | Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center | Treatment of peritoneal injury using jak inhibitors |
| KR20160117850A (en) * | 2015-03-31 | 2016-10-11 | 중앙대학교 산학협력단 | Method for preparing lectin with reduced blood coagulation activity |
Non-Patent Citations (1)
| Title |
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| 杨光等: "三七茎叶、三七花新食品原料研究", 《中国药学杂志》, vol. 52, no. 7, pages 543 - 547 * |
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