CN115152524A - Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder - Google Patents

Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder Download PDF

Info

Publication number
CN115152524A
CN115152524A CN202110291259.6A CN202110291259A CN115152524A CN 115152524 A CN115152524 A CN 115152524A CN 202110291259 A CN202110291259 A CN 202110291259A CN 115152524 A CN115152524 A CN 115152524A
Authority
CN
China
Prior art keywords
zinc
rich
selenium
culture medium
organic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202110291259.6A
Other languages
Chinese (zh)
Inventor
于晓
张振宇
唐尉豪
姚亚军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Xiangyu Seed Technology Co ltd
Original Assignee
Shandong Xiangyu Seed Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Xiangyu Seed Technology Co ltd filed Critical Shandong Xiangyu Seed Technology Co ltd
Priority to CN202110291259.6A priority Critical patent/CN115152524A/en
Publication of CN115152524A publication Critical patent/CN115152524A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D13/00Finished or partly finished bakery products
    • A21D13/06Products with modified nutritive value, e.g. with modified starch content
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/36Vegetable material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/152Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/156Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16Inorganic salts, minerals or trace elements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/117Flakes or other shapes of ready-to-eat type; Semi-finished or partly-finished products therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/20Agglomerating; Granulating; Tabletting
    • A23P10/28Tabletting; Making food bars by compression of a dry powdered mixture
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/30Encapsulation of particles, e.g. foodstuff additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/40Shaping or working of foodstuffs characterised by the products free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The embodiment of the disclosure provides a cultivation method of zinc-rich and selenium-rich shiitake mushrooms, a method for preparing shiitake mushroom nutrition powder, and health-care products and foods comprising the shiitake mushroom nutrition powder. The cultivation method comprises the following steps: mixing a culture medium with inorganic or organic zinc and organic selenium, sterilizing, inoculating a mushroom strain, and culturing to obtain mushroom, wherein the mass ratio of the inorganic or organic zinc to dry materials of the culture medium is 0.02-2.

Description

Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder
Technical Field
The embodiment of the disclosure relates to a cultivation method of zinc-rich and selenium-rich shiitake mushrooms, a method for preparing shiitake mushroom nutrition powder, the shiitake mushroom nutrition powder, and health-care products and foods comprising the shiitake mushroom nutrition powder.
Background
Lentinus edodes, also known as Lentinus edodes and Lentinus edodes, is an edible fungus. In 2018, the total yield of the mushrooms in China is 1042.12 ten thousand tons (accounting for 27.5 percent of the total yield of the edible mushrooms), and the mushrooms are positioned at the first position of the edible mushrooms. The mushroom is fungus food with high protein, low fat, polysaccharide, various amino acids and various vitamins, and also contains various vitamins and mineral substances, and has great effects of promoting metabolism and improving organism adaptability. In addition, the shiitake mushroom is rich in dietary fiber, and can reduce cholesterol in blood and prevent atherosclerosis by eating the shiitake mushroom frequently. Therefore, the nutritional value and the medicinal value of the mushroom are very high.
Selenium (Se) is called as cancer prevention king in human body microelements by scientists. Selenium is one of essential trace elements for human body, and the selenium deficiency of human body can cause dysfunction of some important organs, resulting in occurrence of a plurality of serious diseases. Currently, there are over 40 countries in the world that are selenium deficient, and several hundred million people in 22 provinces of china are in selenium deficient or low selenium zones.
Zinc (Zn) is also one of the essential trace elements of human body, and plays an extremely important role in the important physiological processes of human body growth and development, reproductive inheritance, immunity, endocrine and the like. Zinc deficiency easily causes inappetence, immunologic function decline, reproductive system function impairment, slow wound healing, cold, abortion and other problems.
Disclosure of Invention
According to the embodiment of the disclosure, a cultivation method of zinc-rich and selenium-rich shiitake mushrooms is provided, which comprises the following steps: mixing a culture medium with inorganic or organic zinc and organic selenium in percentage by mass, sterilizing, inoculating a mushroom strain, and culturing to obtain mushroom, wherein the mass ratio of the inorganic or organic zinc to dry materials of the culture medium is 0.02-2.
For example, the medium is selected from one of the following formulations:
the formula 1, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 75-85% of wood dust, 12-17% of bran, 1-5% of soybean meal, 1-5% of rice bran, 0.1-0.5% of glucose, 0.1-0.5% of gypsum and 0.1-0.5% of lime;
the formula 2, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 14-20% of bran, 0.1-1% of gypsum and 0.1-1% of lime;
and in the formula 3, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 12-17% of bran, 1-5% of soybean meal, 1-5% of corn flour, 0.1-1% of gypsum and 0.1-1% of lime;
and 4, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 50-60% of wood chips, 20-40% of cottonseed hulls, 12-17% of bran, 5-10% of corn flour, 0.1-1% of gypsum and 0.1-1% of lime;
and 5, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-80% of wood chips, 12-17% of bran, 5-10% of corncobs, 0.1-1% of gypsum and 0.1-1% of lime;
and the formula 6 is that the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-80% of wood chips, 12-17% of bran, 5-10% of rice bran, 0.1-1% of gypsum and 0.1-1% of lime; and
and the formula 7, wherein the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 14-20% of bran, 0.1-1% of gypsum, 0.1-1% of lime, 0.1-1% of magnesium sulfate and 0.1-1% of monopotassium phosphate.
For example, the inorganic zinc is selected from at least one of zinc chloride, zinc sulfate, zinc carbonate, zinc nitrate, and zinc oxide.
For example, the zinc sulfate is zinc sulfate heptahydrate or zinc sulfate hexahydrate.
For example, the organic zinc is selected from at least one of zinc gluconate and zinc methionine.
For example, the mass ratio of the inorganic or organic zinc to the dry matter of the culture medium is 0.02 to 0.5.
For example, the mass ratio of the inorganic or organic zinc to the dry matter of the culture medium is 0.05.
For example, the organic selenium is selected from at least one of selenoprotein, selenium-enriched yeast and selenium malt.
For example, the organic selenium is selenium malt.
For example, the mass ratio of the organic selenium to the dry materials of the culture medium is 0.5:100 or 1.5.
For example, the cultivation method of the zinc-rich and selenium-rich shiitake mushrooms further comprises the following steps: adjusting the water content of the culture medium to 50-60% after mixing the culture medium with the inorganic or organic zinc and the organic selenium.
According to the embodiment of the disclosure, a method for preparing mushroom nutrition powder by using zinc-rich and selenium-rich mushrooms cultivated by the method comprises the following steps: collecting fruiting body after fruiting, drying, pulverizing, sieving, and extracting with ultrasonic technology.
For example, the drying process is to dry the collected fruit body in a sterile environment.
For example, the drying temperature is 30 to 40 ℃.
According to the embodiment of the disclosure, the shiitake mushroom nutrition powder is prepared from the zinc-rich selenium-rich shiitake mushrooms cultivated by the method.
According to an embodiment of the disclosure, a health product comprising the shiitake mushroom nutrition powder is provided, wherein the health product has a dosage form of any one of the following: powder, tablet granules, gel, lotion or cream.
According to an embodiment of the present disclosure, there is provided a food including the shiitake mushroom nutritional powder as described above, wherein the food is a biscuit, a milk powder, a noodle or a ready-to-eat food.
Drawings
To more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below, and it is apparent that the drawings in the following description relate only to some embodiments of the present disclosure and are not limiting to the present disclosure.
FIG. 1 is a statistical chart of fruiting yields of examples 1-3 and comparative example 1.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without any inventive step, are within the scope of protection of the disclosure.
It should be noted that those who do not specify specific conditions in the examples of the present disclosure proceed according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The embodiment of the disclosure provides a cultivation method of zinc-rich and selenium-rich shiitake mushrooms, which comprises the following steps: mixing the culture medium with inorganic or organic zinc and organic selenium, sterilizing, inoculating Lentinus Edodes strain, and culturing to obtain Lentinus Edodes; wherein, the mass ratio of inorganic or organic zinc to the dry materials of the culture medium is 0.02-2:100, the mass ratio of the organic selenium to the dry materials of the culture medium is 0.1-3.
According to the embodiment of the disclosure, inorganic or organic zinc and organic selenium are mixed in a culture medium, selenium and zinc are enriched in mushroom fruiting bodies through hypha of mushroom strains, and the selenium and zinc are converted into organic elements which are easy to absorb and utilize by a human body. The cultivated lentinus edodes is milled to obtain the lentinus edodes nutrition powder simultaneously enriched with organic selenium and organic zinc.
For example, the medium is selected from one of the following formulations:
the formula 1, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 75-85% of wood dust, 12-17% of bran, 1-5% of soybean meal, 1-5% of rice bran, 0.1-0.5% of glucose, 0.1-0.5% of gypsum and 0.1-0.5% of lime;
the formula 2, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 14-20% of bran, 0.1-1% of gypsum and 0.1-1% of lime;
and a formula 3, wherein the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 12-17% of bran, 1-5% of soybean meal, 1-5% of corn flour, 0.1-1% of gypsum and 0.1-1% of lime;
and 4, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 50-60% of wood chips, 20-40% of cottonseed hulls, 12-17% of bran, 5-10% of corn flour, 0.1-1% of gypsum and 0.1-1% of lime;
and 5, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-80% of wood chips, 12-17% of bran, 5-10% of corncobs, 0.1-1% of gypsum and 0.1-1% of lime;
and the formula 6 is that the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-80% of wood chips, 12-17% of bran, 5-10% of rice bran, 0.1-1% of gypsum and 0.1-1% of lime; and
and the formula 7, wherein the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 14-20% of bran, 0.1-1% of gypsum, 0.1-1% of lime, 0.1-1% of magnesium sulfate and 0.1-1% of monopotassium phosphate.
In addition, the inventors found that when the culture medium of the above formulation is selected and inorganic or organic zinc and organic selenium are mixed into the culture medium according to the above mass ratio to obtain a mixture for shiitake cultivation, the yield of shiitake mushroom is high in both tides. The adding amount of the organic selenium and the inorganic or organic zinc is high during cultivation, which is beneficial to keeping the content of the organic selenium and the organic zinc in the nutrition powder prepared after fruiting at a high level. The nutrition powder prepared from the shiitake mushrooms cultivated by the cultivation method provided by the embodiment of the disclosure has the characteristics of high purity, rich nutrition, selenium enrichment and zinc enrichment.
In addition, the cultivation method provided by the embodiment of the disclosure is simple and easy to implement, the production cost is low, the mushroom nutrition powder prepared from the mushrooms cultivated by the method of the embodiment of the disclosure is safe and environment-friendly, can be used as a food additive, a health food additive and the like, and has the advantages of safety, organic property, no pollution, nutrition and health care effects and the like.
For example, the inorganic zinc is at least one selected from the group consisting of zinc chloride, zinc sulfate, zinc carbonate, zinc nitrate and zinc oxide. Preferably, the zinc sulfate is zinc sulfate heptahydrate or zinc sulfate hexahydrate.
For example, the organic zinc is at least one selected from zinc gluconate and zinc methionine.
In other embodiments, the type of inorganic or organic zinc may be adaptively selected according to the type of the cultured shiitake mushroom and the level of zinc enrichment of the hyphae, and is not limited to the types of inorganic or organic zinc defined in the examples of the present disclosure. Preferably, the inorganic zinc is selected from zinc sulphate heptahydrate.
For example, the mass ratio of the inorganic or organic zinc to the dry materials of the culture medium is 0.02-0.5.
For example, the mass ratio of the inorganic or organic zinc to the dry matter of the culture medium is 0.05.
When the mass ratio of the inorganic or organic zinc to the culture medium is within the above range, the content of the organic zinc in the shiitake nutrition powder prepared from the cultivated shiitake is at a high level.
For example, the organic selenium is at least one selected from selenoprotein, selenium-enriched yeast and selenium malt. Preferably, the organic selenium is selected from selenium malt.
Compared with inorganic selenium such as sodium selenite, the selenium malt has the advantages of edible safety, less toxic and side effects, high absorption and utilization rate and the like. The selenium malt is a highly safe high-efficiency natural organic selenium product, and has high biological activity and good absorption and utilization rate. In addition, selenium malt has a superior anticancer effect.
For example, the mass ratio of the organic selenium to the dry materials of the culture medium is 0.5:100 or 1.5.
It should be noted that the water content of the dry material of the culture medium is approximately equal to zero.
For example, the cultivation method further includes: the water content of the culture medium is adjusted to 50-60% after the culture medium is mixed with inorganic or organic zinc and organic selenium.
For example, the mushroom strain is the mushroom strain qihe No. 2 provided by qihe biotechnology limited of shandong. However, the disclosed embodiments are not limited thereto. In other embodiments, the Lentinus edodes strain can be selected from Lentinus edodes 939, 937, 135-5,L-26, 868, 931, wu Xiang I, lentinus edodes 241-4, lentinus edodes 303, lentinus edodes 808, shenxiang 215, lentinus edodes 9608, lentinus edodes SD-1 or Luchou 7579 Lentinus edodes.
According to the embodiment of the disclosure, a method for preparing the mushroom nutrition powder by using the zinc-rich and selenium-rich mushrooms cultivated by the method is also provided, which comprises the following steps: collecting fruiting body after fruiting, drying, pulverizing, sieving, and extracting with ultrasonic technology. The water content of the fruit body is reduced by drying treatment, so as to facilitate the subsequent pulverization.
For example, the drying treatment is to dry the collected fruit body in a sterile environment; preferably, the drying temperature is 30-40 ℃.
For example, milling may be followed by sieving and extraction using ultrasonic techniques. By using the ultrasonic extraction technology, the lentinus edodes nutrition powder which is high in purity, rich in nutrition and rich in organic selenium and organic zinc is produced. For example, the sieving may be selected from 100 mesh sieving.
According to the embodiment of the disclosure, the shiitake mushroom nutrition powder is prepared from the zinc-rich selenium-rich shiitake mushrooms cultivated according to the cultivation method of the disclosed embodiment.
According to the embodiment of the disclosure, a health product comprising the mushroom nutrition powder is also provided, for example, the health product has any one of the following dosage forms: powder, tablet granules, gel, lotion or cream.
According to an embodiment of the present disclosure, a food including the mushroom nutrition powder is also provided, for example, the food is a biscuit, milk powder, noodles or instant food.
The lentinus edodes cultivated by the cultivation method disclosed by the embodiment of the disclosure can be used for preparing lentinus edodes nutrition powder, and the lentinus edodes nutrition powder can be used for preparing powder and tablet health products, and can also be added into health products, vegetables, crops, biscuits, milk powder, noodles, instant food and the like.
In addition, because the planting area of the mushrooms in China is wide, the mushroom cultivation method provided by the embodiment of the disclosure can improve the additional value of mushroom products and increase the income of farmers. The mushroom cultivation method according to the embodiment of the disclosure is safe, environment-friendly and pollution-free, and the prepared product contains cadmium, arsenic, lead and total mercury elements within the national standard range, and is a natural organic food.
Hereinafter, the cultivation method of the zinc-rich selenium-rich shiitake mushrooms according to the embodiments of the present disclosure will be further explained by way of examples.
Example 1
The example provides a cultivation method of zinc-rich and selenium-rich shiitake mushrooms. The culture medium of the mushroom strain comprises the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum and 0.5% of lime, wherein 0.5g of zinc sulfate heptahydrate and 5g of selenium malt are added into each kilogram of dry materials of the culture medium. In this example, selenium malt was mainly prepared from food-grade selenium malt, which was commercially available. Thereafter, the water content of the medium was adjusted to 60%.
The cultivation method of the zinc-rich and selenium-rich shiitake mushrooms comprises the following steps:
preparing a culture medium by taking the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum (calcium sulfate hydrate) and 0.5% of lime (calcium oxide). Adding 0.5g of zinc sulfate heptahydrate and 5g of selenium malt into each kilogram of dry materials of the culture medium to prepare a culture mixture, and adjusting the water content of the culture medium to 60%. And bagging, sealing and sterilizing the culture mixture, then inoculating the No. 2 strain of the seven river, and culturing until the mushroom grows.
For example, the collected fruiting bodies are dried in a sterile room after fruiting. The drying is carried out by using an oven at 30 ℃. Then pulverizing, sieving with 100 mesh sieve, and extracting by ultrasonic technology to obtain selenium-rich and zinc-rich organic Lentinus Edodes powder.
Example 2
The example provides a cultivation method of zinc-rich and selenium-rich shiitake mushrooms. The culture medium of the mushroom strain comprises the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum and 0.5% of lime. Zinc sulfate heptahydrate weighing 1g and selenium malt weighing 10g are added into dry materials of each kilogram of culture medium. In this example, selenium malt is mainly food-grade selenium malt. Thereafter, the water content of the medium was adjusted to 60%.
The cultivation method of the zinc-rich and selenium-rich shiitake mushrooms comprises the following steps:
preparing a culture medium by taking the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum and 0.5% of lime. Adding zinc sulfate heptahydrate weighing 1g and selenium malt weighing 10g into dry materials of each kilogram of culture medium to obtain a culture mixture, and adjusting the water content of the culture medium to 60%. Bagging the culture mixture, sealing, sterilizing, inoculating No. 2 strain of QIHE, and culturing to obtain fruiting body.
For example, the collected fruiting bodies are dried in a sterile room after fruiting. The drying is carried out by using an oven at 30 ℃. Then pulverizing, sieving with 100 mesh sieve, and extracting by ultrasonic technology to obtain selenium-rich and zinc-rich organic Lentinus Edodes powder.
Example 3
The example provides a cultivation method of zinc-rich and selenium-rich shiitake mushrooms. The culture medium of the mushroom strain comprises the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum and 0.5% of lime. Zinc sulfate heptahydrate weighing 1.5g and selenium malt weighing 15g are added to each kilogram of dry culture medium. In this example, selenium malt is mainly food-grade selenium malt. Thereafter, the water content of the medium was adjusted to 60%.
The cultivation method of the zinc-rich and selenium-rich shiitake mushrooms comprises the following steps:
preparing a culture medium by taking the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum and 0.5% of lime. Adding zinc sulfate heptahydrate weighing 1.5g and selenium malt weighing 15g into dry materials of each kilogram of culture medium to obtain a culture mixture, and adjusting the water content of the culture medium to 60%. Bagging the culture mixture, sealing, sterilizing, inoculating No. 2 strain of QIHE, and culturing to obtain fruiting body.
For example, the collected fruiting bodies are dried in a sterile room after fruiting. The drying is carried out by using an oven at 30 ℃. Then pulverizing, sieving with 100 mesh sieve, and extracting with ultrasonic technology to obtain selenium-rich and zinc-rich organic Lentinus Edodes powder.
Comparative example 1
The comparative example provides a cultivation method of zinc-rich and selenium-rich shiitake mushrooms. The culture medium of the mushroom strain comprises the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum, 0.5% of lime and 60% of water content of a regulating culture medium. In this comparative example, inorganic zinc and organic selenium were not added.
The cultivation method of the zinc-rich and selenium-rich shiitake mushrooms comprises the following steps:
preparing a culture medium by taking the following raw materials in percentage by mass: 75% of wood chips, 14% of bran, 5% of soybean meal, 4.5% of rice bran, 0.5% of glucose, 0.5% of gypsum, 0.5% of lime and 60% of water content of an adjusting culture medium. Bagging the culture medium, sealing, sterilizing, inoculating No. 2 strain of QIHE, and culturing to obtain fruiting body.
For example, the collected fruiting bodies are dried in a sterile room after fruiting. The drying is carried out by using an oven at 30 ℃. Then pulverizing, sieving with 100 mesh sieve, and extracting with ultrasonic technology to obtain Lentinus Edodes powder.
It should be noted that the powdering and ultrasonic extraction processes in comparative example 1 and examples 1 to 3 were the same.
Experimental example 1
The prepared mushroom powder of examples 1 to 3 and comparative example 1 was subjected to the measurement of the contents of organic selenium and organic zinc. Specifically, the contents of organic selenium and organic zinc in the mushroom powder prepared from shiitake mushroom at the 1 st tide were measured according to the measurement methods described in national standard GB5009.93-2003, "method for measuring selenium in food", and GB5009.268-2016, "method for measuring multiple elements in food", and the measurement data are shown in table 1. In the experimental example, the pileus and the stipe are respectively pulverized to detect the contents of organic selenium and organic zinc in the pileus and the stipe.
Table 1: examples 1-3 and comparative example 1 data for the determination of organoselenium and organozinc
Figure BDA0002982062090000091
It can be seen from table 1 that the contents of organic selenium and organic zinc in the pileus and stipe lentinus edodes powder are significantly higher than that in comparative example 1 after zinc sulfate heptahydrate and selenium malt are added to the culture medium. The contents of organic selenium and organic zinc in the pileus are higher than those in the stipe, which shows that the capability of the pileus for enriching the organic selenium and the organic zinc is higher than that of the stipe. The content of the organic zinc of the pileus in the example 3 is up to 98.2mg/kg, and the content of the organic selenium of the pileus in the example 2 is up to 135mg/kg.
FIG. 1 is data of fruiting yields of examples 1 to 3 and comparative example 1, and it can be seen from FIG. 1 that the fruiting yields of two seasons are higher after adding zinc sulfate heptahydrate and selenium malt to the medium than in the comparative example. The fact that the culture medium is added with the zinc sulfate heptahydrate and the selenium malt is beneficial to improving the fruiting yield.
When 1g/kg of zinc sulfate heptahydrate and 10g/kg of selenium malt are added into the culture medium, the highest fruiting yield reaches 715g.
Experimental example 2
The experimental example performs food safety evaluation on the prepared pileus and stipe mushroom powder. The contents of cadmium, arsenic, lead and mercury in the prepared pileus and stipe mushroom powder are measured. Table 2 shows the data of the determination of cadmium and arsenic in the pileus and stipe mushroom powders prepared in examples 1-3 and comparative example 1.
Table 2: examples 1-3 and comparative example 1 cadmium and arsenic determination data.
Figure BDA0002982062090000101
As can be seen from Table 2, the cadmium content and the arsenic content of the pileus and stipe lentinus edodes powder prepared by adding zinc sulfate heptahydrate and selenium malt are within the range of 0.203-0.431mg/kg and within the range of 0.185-0.433mg/kg, which do not exceed the national standard, and the cadmium content and the arsenic content of the pileus are slightly higher than those of stipes.
Table 3 shows the data of the elemental lead and mercury in the pileus and stipe of examples 1-3 and comparative example 1. As can be seen from Table 3, the lead content and the mercury content of the pileus and the stipe mushroom powder prepared by adding zinc sulfate heptahydrate and selenium malt are within the range of 0.06-0.1mg/kg and within the range of 0.009-0.014mg/kg, which do not exceed the national standard, and the lead content and the mercury content of the pileus and the stipe content are not obviously different.
Table 3: the data of the lead and mercury elements in examples 1-3 and comparative example 1.
Figure BDA0002982062090000111
The embodiment of the disclosure provides a cultivation method of zinc-rich and selenium-rich shiitake mushrooms, which is characterized in that selenium and zinc elements are simultaneously enriched in the shiitake mushrooms by utilizing the hypha enrichment effect of shiitake mushroom strains. The cultivation method is safe, environment-friendly and pollution-free. The lentinus edodes nutrition powder rich in organic selenium and organic zinc is prepared by pulverizing lentinus edodes, and cadmium, arsenic, lead and mercury elements in the lentinus edodes nutrition powder are all in the national standard range, so that the lentinus edodes nutrition powder is a natural organic food. The lentinus edodes nutrition powder provided by the embodiment of the disclosure can be prepared into powder and tablet health care products, and can also be added into health care products, vegetables, crops, biscuits, milk powder, noodles or instant food.
The above description is only exemplary of the present disclosure, and is not intended to limit the scope of the present disclosure, which is defined by the appended claims. Various modifications and alterations to this disclosure may occur to those skilled in the art; any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present disclosure should be included in the protection scope of the present disclosure.

Claims (17)

1. A cultivation method of zinc-rich and selenium-rich shiitake mushrooms comprises the following steps: mixing the culture medium with inorganic or organic zinc and organic selenium, sterilizing, inoculating Lentinus Edodes strain, culturing to obtain mushroom,
the mass ratio of the inorganic or organic zinc to the dry materials of the culture medium is 0.02-2.
2. The cultivation method of the zinc-rich selenium-rich shiitake mushroom as claimed in claim 1, wherein the culture medium is selected from one of the following formulas:
the formula 1, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 75-85% of wood dust, 12-17% of bran, 1-5% of soybean meal, 1-5% of rice bran, 0.1-0.5% of glucose, 0.1-0.5% of gypsum and 0.1-0.5% of lime;
the formula 2 is that the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 14-20% of bran, 0.1-1% of gypsum and 0.1-1% of lime;
and in the formula 3, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 12-17% of bran, 1-5% of soybean meal, 1-5% of corn flour, 0.1-1% of gypsum and 0.1-1% of lime;
and 4, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 50-60% of wood chips, 20-40% of cottonseed hulls, 12-17% of bran, 5-10% of corn flour, 0.1-1% of gypsum and 0.1-1% of lime;
and 5, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-80% of wood chips, 12-17% of bran, 5-10% of corncobs, 0.1-1% of gypsum and 0.1-1% of lime;
and the formula 6 is that the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-80% of wood chips, 12-17% of bran, 5-10% of rice bran, 0.1-1% of gypsum and 0.1-1% of lime; and
and 7, the dry materials of the culture medium comprise the following raw materials in percentage by mass: 70-85% of wood chips, 14-20% of bran, 0.1-1% of gypsum, 0.1-1% of lime, 0.1-1% of magnesium sulfate and 0.1-1% of monopotassium phosphate.
3. The cultivation method of zinc-rich selenium-rich shiitake mushroom according to claim 1, wherein the inorganic zinc is at least one selected from the group consisting of zinc chloride, zinc sulfate, zinc carbonate, zinc nitrate and zinc oxide.
4. The cultivation method of the zinc-rich selenium-rich shiitake mushroom according to claim 3, wherein the zinc sulfate is zinc sulfate heptahydrate or zinc sulfate hexahydrate.
5. The cultivation method of zinc-rich selenium-rich shiitake mushrooms according to claim 1, wherein the organic zinc is selected from at least one of zinc gluconate and zinc methionine.
6. The cultivation method of the zinc-rich selenium-rich shiitake mushroom as claimed in claim 1, wherein the mass ratio of the inorganic or organic zinc to the dry materials of the culture medium is 0.02-0.5.
7. The cultivation method of the zinc-rich selenium-rich shiitake mushroom as claimed in claim 6, wherein the mass ratio of the inorganic or organic zinc to the dry materials of the culture medium is 0.05.
8. The method for cultivating zinc-rich selenium-rich shiitake mushroom according to claim 1, wherein the organic selenium is at least one selected from selenoprotein, selenium-rich yeast and selenium malt.
9. The cultivation method of zinc-rich selenium-rich shiitake mushroom according to claim 8, wherein the organic selenium is selenium malt.
10. The cultivation method of the zinc-rich selenium-rich shiitake mushroom as claimed in claim 1, wherein the mass ratio of the organic selenium to the dry materials of the culture medium is 0.5:100 or 1.5.
11. The cultivation method of the zinc-rich selenium-rich shiitake mushroom as claimed in any one of claims 1 to 10, further comprising: adjusting the water content of the culture medium to 50-60% after mixing the culture medium with the inorganic or organic zinc and the organic selenium.
12. A method for preparing mushroom nutrition powder by using zinc-rich selenium-rich mushrooms cultivated according to any one of claims 1-11, comprising the steps of: collecting fruiting body after fruiting, drying, pulverizing, sieving, and extracting with ultrasonic technology.
13. The method for preparing shiitake mushroom nutritional powder according to claim 12, wherein the drying process is drying the collected fruit bodies in a sterile environment.
14. The method for preparing shiitake mushroom nutritional powder according to claim 13, wherein the drying temperature is 30-40 ℃.
15. A shiitake nutrition powder, wherein the shiitake nutrition powder is prepared from zinc-rich selenium-rich shiitake mushrooms cultivated according to the method of any one of claims 1 to 11.
16. A health product comprising the shiitake mushroom nutritional powder according to claim 15, wherein the health product has a dosage form of any one of: powder, tablet granules, gel, lotion or cream.
17. A food comprising the shiitake mushroom nutritional powder according to claim 15, wherein the food is a biscuit, a milk powder, a noodle or a ready-to-eat food.
CN202110291259.6A 2021-03-18 2021-03-18 Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder Pending CN115152524A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110291259.6A CN115152524A (en) 2021-03-18 2021-03-18 Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110291259.6A CN115152524A (en) 2021-03-18 2021-03-18 Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder

Publications (1)

Publication Number Publication Date
CN115152524A true CN115152524A (en) 2022-10-11

Family

ID=83476001

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110291259.6A Pending CN115152524A (en) 2021-03-18 2021-03-18 Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder

Country Status (1)

Country Link
CN (1) CN115152524A (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101857489A (en) * 2010-05-14 2010-10-13 东至县德丰食用菌科技开发中心 Zinc-enriched selenium-enriched lentinus edodes and cultivation method thereof
CN106105779A (en) * 2016-07-07 2016-11-16 孙备宽 A kind of organic selenium-rich Edible Fungi method
CN108142205A (en) * 2017-12-28 2018-06-12 江西省森旺现代农业生态科技开发有限公司 A kind of selenium-enriched hericium erinaceus breeding method
CN110655592A (en) * 2019-10-31 2020-01-07 贺州学院 Ultrasonic-assisted lentinan extraction method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101857489A (en) * 2010-05-14 2010-10-13 东至县德丰食用菌科技开发中心 Zinc-enriched selenium-enriched lentinus edodes and cultivation method thereof
CN106105779A (en) * 2016-07-07 2016-11-16 孙备宽 A kind of organic selenium-rich Edible Fungi method
CN108142205A (en) * 2017-12-28 2018-06-12 江西省森旺现代农业生态科技开发有限公司 A kind of selenium-enriched hericium erinaceus breeding method
CN110655592A (en) * 2019-10-31 2020-01-07 贺州学院 Ultrasonic-assisted lentinan extraction method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
陈士瑜等: "菇菌生产技术全书", vol. 1, 中国农业出版社, pages: 52 - 54 *

Similar Documents

Publication Publication Date Title
CN102150555B (en) Method for culturing pleurotus geesteranus uniformly rich in selenium by using mixed selenium source
CN105077029A (en) Dinner replacing powder containing zinc-rich barley grass and preparation method of dinner replacing powder
CN102165894B (en) Method for carrying out rich-selenium culturing of plelmtus eqngiu by utilizing sodium selenite
CN102934810A (en) Application of chlorella in foods
CN104557304A (en) Selenium-rich shii-take cultivation material and preparation method thereof
CN102177809B (en) Method for carrying out uniform selenium-enriched cultivation of pleurotus geesteranus by using selenium yeast
CN108271938A (en) A kind of functional seaweed fodder additive improving prevention of sow constipation
CN102150556A (en) Method for cultivating pleurotus eryngii uniformly rich in selenium with mixed organic selenium source
CN101176521B (en) North cordyceps rice flour product and preparation method thereof
CN102150558A (en) Method for culturing pleurotus geesteranus uniformly rich in selenium by utilizing sodium selenate
CN101467689A (en) Organic functional food and preparation method thereof
CN108383652A (en) Agricultural wastes cultivate rich selenium germanium green organic element nutrition fertilizer
CN102960711B (en) Natural nourishment and preparation method thereof
CN105145115B (en) A kind of production method of organic Se-rich zinc-rich product
CN102440359A (en) Health fern root vermicelli and preparation method thereof
CN103039958A (en) Nutritional cereal powder, and preparation and application thereof
CN107279698A (en) One kind is applied to teen-age whole wheat
CN115152524A (en) Cultivation method of zinc-rich and selenium-rich shiitake mushrooms and method for preparing shiitake mushroom nutrition powder
CN102715348A (en) Zinc-enriched formulated hard palletized feed for carp and preparation method thereof
CN109673390A (en) A kind of selenium-enriched cultivation of auricularia auricula matrix and preparation method thereof
CN104961542A (en) Production process for special organic fertilizer used for sweet potato
CN104489683B (en) Black wolfberry maca powder and preparation method thereof
CN113519717B (en) Composite feed additive and feed for laying hens in growing period
CN104522372A (en) Elver compound feed for improving immunity and preparation method of elver compound feed
CN109355243B (en) Preparation method and application of selenium-rich schizophyllum commune for high yield of glucan

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination