CN115141711A - Intelligent bacteria incubator and use method thereof - Google Patents

Intelligent bacteria incubator and use method thereof Download PDF

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Publication number
CN115141711A
CN115141711A CN202210823208.8A CN202210823208A CN115141711A CN 115141711 A CN115141711 A CN 115141711A CN 202210823208 A CN202210823208 A CN 202210823208A CN 115141711 A CN115141711 A CN 115141711A
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culture
plate
incubator
sealing
groove
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李晋
朱小明
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Individual
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
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Abstract

The invention discloses an intelligent bacterial incubator and a use method thereof in the technical field of bacterial culture. According to the invention, a part of the storage assemblies and the culture dish can be taken out conveniently by a worker through the sealing plate, so that bacteria cultured inside can be taken out conveniently, and meanwhile, the storage assemblies or the culture dish can be cleaned conveniently, so that cross contamination is avoided; the arrangement of the blocking assembly can prevent the pollution of outside air to unused culture dishes through the cooperation of the blocking assembly and the sealing plate and the pressing plate when a part of the storage assembly is taken; the sealing plug and the sealing ring can improve the wind tight sealing of the culture chamber under the matching of the packaging plate and the top plate.

Description

Intelligent bacteria incubator and use method thereof
Technical Field
The invention relates to the technical field of bacterial culture, in particular to an intelligent bacterial incubator and a using method thereof.
Background
The bacterial culture refers to a modern biotechnology which enables bacteria to grow and propagate in a culture medium by manually controlling temperature, environment and nutrients, and the bacterial culture is usually carried out in a bacterial incubator.
The invention discloses a part of invention patents in the technical field of bacterial culture, wherein the invention patent with the application number of 201811426666.8 discloses a bacterial culture box, the tightness between a box door and a box body shell can be increased through the arranged sealing gaskets and the arranged magnet blocks, the closing stability of the box door and the box body shell can be increased, and the whole bacterial culture box is simple in structure, convenient to operate and better in using effect compared with a traditional mode.
Technical scheme has improved the compactness that box and chamber door are connected, nevertheless still need open the chamber door when taking partial culture dish, can't avoid the external air to enter into inside the box when opening the chamber door moreover to external environment causes the pollution to its and culture dish that does not take inside the box when can't avoiding taking partial culture dish, thereby influences the growth of bacterium, reduces the reliability of inspection data when the later stage is taken the bacterium experiment.
Based on the above, the invention designs an intelligent bacteria incubator and a using method thereof, so as to solve the problems.
Disclosure of Invention
The invention aims to provide an intelligent bacteria incubator and a using method thereof, and aims to solve the problem that the environment cannot pollute the culture plate and bacteria in the culture plate when a part of the culture plate is taken.
In order to achieve the purpose, the invention provides the following technical scheme: an intelligent bacteria incubator comprises an incubator and an incubator chamber, wherein a plurality of storage assemblies are inserted into the incubator chamber, a blocking assembly located on the back face of each storage assembly is arranged inside the incubator chamber, a storage groove is formed in the top of each storage assembly, a plurality of positioning grooves which are uniformly arranged are formed in the bottom of the inner cavity of each storage groove, culture dishes are placed inside each positioning groove, a positioning sleeve is sleeved outside each culture dish, a pressing plate is fixedly sleeved outside each positioning sleeve, and the total area of the pressing plates is the same as the cross-sectional area of each storage groove;
the sealing and blocking assembly comprises a sealing and blocking plate, the front surface of the sealing and blocking plate is in contact with the storage assembly, the side surface of the sealing and blocking plate is in contact with the culture chamber or the pressing plate, the back surface of the sealing and blocking plate is connected with a return spring, and the rear end of the return spring is connected with the back surface of the inner cavity of the culture chamber;
the storage assembly comprises a culture frame, a sealing plate is connected to the front side of the culture frame, a pull groove is formed in the front side of the sealing plate, a ventilation groove is formed in the bottom of the culture frame, a clamping strip is connected to the side face of the culture frame and is inserted into the culture box, and the tops of the culture frame and the sealing plate are in contact with a blocking plate.
As a further scheme of the invention, the culture shelf is of a breathable rigid filter screen structure, and the sealing plate is of a solid structure.
As a further scheme of the invention, a movable groove positioned at the bottom of the positioning groove is formed in the culture frame, a movable block is sleeved in the movable groove, the bottom of the movable block is connected with a positioning spring, the bottom of the positioning spring is connected with the bottom of an inner cavity of the movable groove, the top of the movable block is fixedly connected with a positioning sleeve, and the inner diameter of the movable block is the same as that of the positioning sleeve.
As a further scheme of the invention, a constant temperature system is arranged on the back of the incubator, a left air inlet cavity and a right air inlet cavity are formed in the incubator, the front side and the rear side of the inner cavity of each air inlet cavity are both connected with a breather pipe, and the breather pipes are mutually communicated with the constant temperature system through hoses.
As a further scheme of the invention, a sealing plug positioned at the front end of the storage assembly is inserted into the culture chamber, the back surface of the sealing plug is in contact with the sealing plate, the front end of the sealing plug extends to the outside of the incubator and is fixedly connected with a packaging plate, the back surface of the packaging plate is connected with a sealing ring positioned on the outer side of the sealing plug, and the back surface of the sealing ring is in contact with the incubator.
As a further scheme of the invention, the left side and the right side of the incubator are both provided with sliding chutes, each part of each sliding chute is connected with a connecting frame through a rotating shaft, the middle part of each connecting frame is in threaded sleeve connection with a screw rod, the back surface of each screw rod penetrates through the connecting frame and is clamped with a top plate, and the top plate is in contact with the packaging plate.
As a further scheme of the invention, the top of the incubator is provided with a touch screen, the interior of the incubator is provided with a controller, and the touch screen is connected with the controller through a wire.
As a further scheme of the invention, a fan, an air supplementing pipe, a humidifier, a dryer, a refrigerator and a heater are arranged in the constant temperature system, an air inlet end of the fan is communicated with part of the vent pipes through a hose, air outlet ends of the refrigerator and the heater are communicated with the other part of the vent pipes through a three-way pipe, the air supplementing pipe is communicated with an air inlet end of the fan, and the air inlet ends of the air supplementing pipe, the humidifier, the dryer, the refrigerator and the heater are all provided with electromagnetic valves.
As a further scheme of the invention, the air outlet end of the fan is communicated with the air inlet ends of the humidifier and the dryer through a three-way pipe, and the air outlet ends of the humidifier and the dryer are communicated with the air inlet ends of the refrigerator and the heater through a four-way pipe.
As a further scheme of the invention, the operation method is as follows:
s1, integrally inserting a plurality of storage assemblies for containing culture dishes and the culture dishes into a culture chamber, and pushing a sealing baffle plate to extrude a return spring under the cooperation of the culture chamber to increase the elastic force recovery performance of the return spring;
s2, inserting the sealing plug into the culture chamber through the packaging plate, enabling the packaging plate and the culture box to clamp the sealing ring, overturning the connecting frame under the matching of the sliding groove and the rotating shaft to enable the connecting frame to overturn to the front end of the packaging plate, rotating the top plate to enable the connecting frame to push the packaging plate through the top plate under the matching of the connecting frame, and clamping the sealing ring under the matching of the culture box, so that culture can be performed;
s3, adjusting the internal environment of the culture room through the cooperation of the touch screen and the controller;
s4, if the subjective mode is selected through the touch screen, sequentially inputting air pressure, humidity and temperature values to be regulated through the touch screen, and automatically starting corresponding equipment and related electromagnetic valves after confirming that the system compares the input values with the detection data of the sensors in the culture room;
s5, if an objective mode is selected, firstly, strains and relevant conditions are selected through the touch screen, then the system automatically starts the fan according to preset data in the controller, compares the preset data with a sensor inside the culture chamber, quickly judges the started humidifier or drier and the refrigerator or heater, and simultaneously starts the electromagnetic valve at the corresponding position, so that the environment (temperature, humidity and air pressure) inside the culture chamber can be quickly and automatically regulated and controlled
S6, after culturing for a period of time, rotating the screw rod to release the locking of the top plate on the packaging plate, turning the connecting frame over the incubator and opening the packaging plate; then pulling out the corresponding storage assembly through the pull groove, and taking out the culture dish of the corresponding part;
s7, the storage assembly can move under the elastic force recovery action of the return spring to push the sealing baffle plate to move along with the storage assembly, and the interior of the culture chamber is sealed again under the matching of the upper and lower adjacent sealing plates and the pressing plate;
s8, press down this moment and take the press plate at culture frame top and can promote position sleeve and movable block downstream and extrude the positioning spring and make its elasticity recovery performance increase, make the position sleeve and press the plate to expose the culture dish so that the experimenter snatchs simultaneously, can take the bacterium that corresponds the culture dish inside.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, a worker can take out part of the storage assemblies and the culture dish conveniently through the sealing plate so as to take out bacteria cultured inside, and meanwhile, the storage assemblies or the culture dish are convenient to clean, so that cross contamination is avoided; the arrangement of the blocking assembly can prevent the pollution of outside air to unused culture dishes through the cooperation of the blocking assembly and the sealing plate and the pressing plate when a part of the storage assembly is taken; the sealing plug and the sealing ring can improve the wind-load tightness of the culture chamber under the matching of the packaging plate and the top plate, so that the pollution of the outside air to the bacteria cultured inside is further reduced, the bacteria culture precision is improved, and the reliability of inspection data is improved.
2. The positions of the plurality of pressing plates can be limited simultaneously through the culture rack, so that the pressing plates can limit a plurality of independent culture dishes through the positioning sleeves, the storage stability of the culture dishes is ensured, the culture rack is convenient to oscillate stably in cooperation with the oscillator, and the culture efficiency is improved; simultaneously can promote position sleeve and movable block extrusion positioning spring downwards through pressing the pressure board to make the position sleeve and the side that exposes the culture dish according to the removal of pressure board under the support of constant head tank to the culture dish, so that the staff takes any culture dish and carries out taking of corresponding bacterium.
3. The cooperation of the constant temperature system and the touch screen can facilitate workers to quickly and automatically regulate and control the environment inside the culture chamber so as to ensure that the internal environment is suitable for the growth of cultured bacteria; also can adjust according to the kind and the quantity of cultivateing the bacterium and external environment and inspection demand etc. through the touch-sensitive screen to reduce the regulation degree of difficulty of temperature, humidity and atmospheric pressure in the cultivation environment in the very big degree, make the experimenter skilled fast the experiment operation to the bacterial cultivation.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic view of the opening of the package plate according to the present invention;
FIG. 3 is a schematic view of the inside of the package panel of the present invention after opening;
FIG. 4 is a schematic view of the storage assembly of the present invention;
FIG. 5 is a side view of the storage assembly of the present invention;
FIG. 6 is a cross-sectional view of the side of the culture dish of the present invention;
FIG. 7 is an enlarged view of a portion of FIG. 6 at A;
FIG. 8 is a schematic view showing the internal structure of the incubator of the present invention;
FIG. 9 is a schematic structural view of the blocking assembly of the present invention;
FIG. 10 is a schematic view of the piping connection of the present invention;
FIG. 11 is a block diagram of the operation of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
part 1, incubator; 2. a culture chamber; 3. a blocking assembly; 31. sealing a baffle plate; 32. a return spring; 4. storing the assembly; 41. a culture shelf; 42. sealing plates; 43. groove drawing; 44. a ventilation slot; 45. a clamping strip; 5. a storage tank; 6. positioning a groove; 7. a culture dish; 8. a positioning sleeve; 9. a pressing plate; 10. a movable groove; 11. a movable block; 12. a positioning spring; 13. a constant temperature system; 131. a fan; 132. air supplementing pipes; 133. a humidifier; 134. a dryer; 135. a refrigerator; 136. a heater; 137. an electromagnetic valve; 14. an air inlet cavity; 15. a breather pipe; 16. a sealing plug; 17. a package board; 18. a seal ring; 19. a chute; 20. a connecting frame; 21. a screw rod; 22. a top plate; 23. a touch screen.
Detailed Description
Referring to fig. 1-11, the invention provides an intelligent bacteria incubator, which comprises an incubator 1 and an incubator 2, wherein a plurality of storage assemblies 4 are inserted into the incubator 2, a blocking assembly 3 positioned on the back of each storage assembly 4 is arranged inside the incubator 2, a storage tank 5 is arranged at the top of each storage assembly 4, a plurality of positioning grooves 6 which are uniformly arranged are arranged at the bottom of the inner cavity of each storage tank 5, culture dishes 7 are placed inside each positioning groove 6, a positioning sleeve 8 is sleeved outside each culture dish 7, a pressing plate 9 is fixedly sleeved outside each positioning sleeve 8, and the total area of the plurality of pressing plates 9 is the same as the cross-sectional area of the storage tank 5;
the blocking assembly 3 comprises a blocking plate 31, the front surface of the blocking plate 31 is in contact with the storage assembly 4, the side surface of the blocking plate 31 is in contact with the culture chamber 2 or the pressing plate 9, the back surface of the blocking plate 31 is connected with a return spring 32, and the rear end of the return spring 32 is connected with the back surface of the inner cavity of the culture chamber 2;
the storage assembly 4 comprises a culture rack 41, a sealing plate 42 is connected to the front surface of the culture rack 41, a pull groove 43 is formed in the front surface of the sealing plate 42, a ventilation groove 44 is formed in the bottom of the culture rack 41, a clamping strip 45 is connected to the side surface of the culture rack 41, the clamping strip 45 is inserted into the incubator 1, and the tops of the culture rack 41 and the sealing plate 42 are in contact with the blocking plate 31;
can push through the subassembly 3 that blocks and seal baffle 31 and follow to take and deposit subassembly 4 and remove under reset spring 32's elasticity recovery effect, when guaranteeing to take out partial storage assembly 4, can be under the contact cooperation of block board 31, culture shelf 41 and pressing board 9, improve the leakproofness between culture chamber 2 inside it and storage assembly 4 to a great extent, reduce the probability that outside air enters into not taking culture dish 7 inside, reduce the influence to continuing to cultivate the bacterium.
As shown in FIG. 4, the culture shelf 41 is a gas-permeable rigid filter screen structure, and the sealing plate 42 is a solid structure;
the sealing plate 42 can ensure the sealing performance inside the culture chamber 2 under the matching of the blocking plate 31 and the pressing plate 9, the culture shelf 41 can ensure the flow of air inside the culture chamber 2, and the constant temperature system 13 can ensure the regulation and control of the environment around the culture dish 7 under the matching of the culture shelf 41 and the ventilation groove 44 to ensure the growth of bacteria.
As shown in fig. 7, a movable groove 10 located at the bottom of the positioning groove 6 is formed in the cultivation shelf 41, a movable block 11 is sleeved in the movable groove 10, a positioning spring 12 is connected to the bottom of the movable block 11, the bottom of the positioning spring 12 is connected to the bottom of the inner cavity of the movable groove 10, the top of the movable block 11 is fixedly connected to the positioning sleeve 8, and the inner diameter of the movable block 11 is the same as the inner diameter of the positioning sleeve 8;
make reciprocating block 11 and position sleeve 8 can be stable reciprocate under the restriction of activity groove 10 to make position sleeve 8 and movable block 11 reciprocating motion under the elasticity recovery effect of positioning spring 12, in order to ensure that position sleeve 8 can restrict culture dish 7 side, avoid the condition that culture dish 7 rocked at will, ensure that culture dish 7 only moves under the effect of oscillator, so that the accuse shakes the effect, reaches the effect of control bacterial growth.
As shown in fig. 8, a constant temperature system 13 is arranged on the back of the incubator 1, a left air inlet cavity 14 and a right air inlet cavity 14 are arranged in the incubator 2, air pipes 15 are connected to the front side and the rear side of the inner cavity of each air inlet cavity 14, and the air pipes 15 are communicated with the constant temperature system 13 through hoses;
the air inlet cavity 14 can ensure the flow of air inside the culture chamber 2 and can circulate through the cooperation of the vent pipe 15 and the constant temperature system 13, and simultaneously, the constant temperature system 13 can control the parameters of the gas environment inside the culture chamber 2 in real time.
As shown in fig. 2, a sealing plug 16 is inserted into the culture chamber 2 and located at the front end of the storage assembly 4, the back of the sealing plug 16 is in contact with a sealing plate 42, the front end of the sealing plug 16 extends to the outside of the incubator 1 and is fixedly connected with a packaging plate 17, the back of the packaging plate 17 is connected with a sealing ring 18 located at the outer side of the sealing plug 16, and the back of the sealing ring 18 is in contact with the incubator 1;
can block up cultivateing room 2 through sealing plug 16, reduce the inside that the external gas enters into cultivateing room 2 to a great extent, and can press from both sides tight sealing washer 18 under the cooperation of incubator 1 through encapsulation board 17, can further reduce the external gas and enter into the inside condition of cultivateing room 2 to effectively improve the sealed effect of encapsulation board 17 to incubator 1 and cultivates room 2.
As shown in fig. 1, the left side and the right side of the incubator 1 are both provided with sliding chutes 19, each part of the sliding chute 19 is connected with a connecting frame 20 through a rotating shaft, the middle part of the connecting frame 20 is in threaded sleeve connection with a screw rod 21, the back surface of the screw rod 21 penetrates through the connecting frame 20 and is clamped with a top plate 22, and the top plate 22 is in contact with the packaging plate 17;
the connecting frame 20 is turned over to the front end of the packing plate 17 by the cooperation of the slide groove 19 and the rotating shaft, and then the top plate 22 is rotated to push the packing plate 17 through the top plate 22 by the cooperation of the connecting frame 20 and to clamp the sealing ring 18 by the cooperation of the incubator 1.
As shown in fig. 1, a touch screen 23 is arranged at the top of the incubator 1, a controller is arranged inside the incubator 1, and the touch screen 23 is connected with the controller through a wire;
the operation of the constant temperature system 13 can be controlled by the cooperation of the touch screen 23 and the controller, and the regulation and control mode of the environmental data is selected by the touch screen 23.
As shown in fig. 10, a fan 131, an air supply pipe 132, a humidifier 133, a dryer 134, a refrigerator 135 and a heater 136 are arranged inside the thermostatic system 13, an air inlet end of the fan 131 is communicated with a part of the vent pipes 15 through a hose, air outlet ends of the refrigerator 135 and the heater 136 are communicated with another part of the vent pipes 15 through a tee pipe, the air supply pipe 132 is communicated with an air inlet end of the fan 131, and air inlet ends of the air supply pipe 132, the humidifier 133, the dryer 134, the refrigerator 135 and the heater 136 are all provided with an electromagnetic valve 137;
the operation of the blower 131, the humidifier 133, the dryer 134, the refrigerator 135 and the heater 136 can be controlled in real time by the controller through the electromagnetic valve 137 under the cooperation of the sensors, so that the internal environment of the incubation chamber 2 can be automatically controlled.
As shown in fig. 10, the air outlet end of the blower 131 is communicated with the air inlet ends of the humidifier 133 and the dryer 134 through a tee pipe, and the air outlet ends of the humidifier 133 and the dryer 134 are communicated with the air inlet ends of the refrigerator 135 and the heater 136 through a four-way pipe.
As shown in fig. 1-11, the method of operation is as follows:
s1, integrally inserting a plurality of storage assemblies 4 for containing culture dishes and the culture dishes 7 into a culture chamber 2, and pushing a sealing baffle plate 31 to enable the sealing baffle plate to extrude a return spring 32 under the cooperation of the culture chamber 2 so as to enable the elastic recovery performance of the sealing baffle plate to be improved;
s2, inserting the sealing plug 16 into the culture chamber 2 through the packaging plate 17, enabling the packaging plate 17 and the culture box 1 to clamp the sealing ring 18, turning over the connecting frame 20 under the matching of the sliding groove 19 and the rotating shaft to enable the connecting frame 20 to be turned over to the front end of the packaging plate 17, then rotating the top plate 22 to enable the connecting frame 20 to push the packaging plate 17 through the top plate 22 under the matching of the connecting frame 20, and clamping the sealing ring 18 under the matching of the culture box 1, so that culture can be conducted;
s3, adjusting the internal environment of the culture chamber 2 through the matching of the touch screen 23 and the controller,
s4, if the subjective mode is selected through the touch screen 23, sequentially inputting air pressure, humidity and temperature values to be regulated through the touch screen 23, and after confirming that the system automatically opens corresponding equipment and related electromagnetic valves 137 after comparing the input values through the detection data of the sensor in the culture chamber 2;
s5, if an objective mode is selected, firstly, strains and relevant conditions are selected through the touch screen 23, then the system automatically starts the fan 131 according to preset data in the controller, compares the preset data with a sensor in the culture chamber 2, quickly judges whether the started humidifier 133 or drier 134 and the refrigerator 135 or heater 136 are started, and simultaneously starts the electromagnetic valve 137 at the corresponding position, so that the environmental temperature, humidity and air pressure in the culture chamber 2 can be quickly and automatically regulated and controlled
S6, after culturing for a period of time, rotating the screw rod 21 to unlock the packaging plate 17 from the top plate 22, turning the connecting frame 20 over the incubator 1, and opening the packaging plate 17; then the corresponding storage assembly 4 is pulled out through the pull groove 43, and the culture dish 7 of the corresponding part can be taken out;
s7, due to the movement of the storage assembly 4, the sealing baffle plate 31 can be pushed under the elastic force recovery action of the return spring 32 to move along with the storage assembly 4, and the interior of the culture chamber 2 is sealed again under the matching of the upper and lower adjacent sealing plates 42 and the pressing plate 9;
s8. The board 9 of pressing down at this moment and taking culture rack 41 top can promote position sleeve 8 and movable block 11 downstream and extrude positioning spring 12 and make its elasticity recovery performance increase, makes position sleeve 8 and press board 9 to expose culture dish 7 so that the experimenter snatchs simultaneously, can get and use the bacterium that corresponds culture dish 7 inside.
The working principle is as follows:
inserting a plurality of storage assemblies 4 for containing culture dishes into the culture chamber 2, pushing a sealing plate 31 to enable the sealing plate to extrude a return spring 32 under the coordination of the culture chamber 2 to enable the elastic recovery performance of the sealing plate to be increased, inserting a sealing plug 16 into the culture chamber 2 through a packaging plate 17, enabling the packaging plate 17 and the culture box 1 to clamp a sealing ring 18, turning a connecting frame 20 to enable the connecting frame 20 to be turned to the front end of the packaging plate 17 under the coordination of a sliding groove 19 and a rotating shaft, rotating a top plate 22 to enable the connecting frame 20 to push the packaging plate 17 through the top plate 22 and clamp the sealing ring 18 under the coordination of the culture box 1;
when a part of the culture dishes 7 in the storage assembly 4 need to be taken out, the locking of the top plate 22 on the packaging plate 17 is firstly released, the connecting frame 20 is turned over to the upper part of the incubator 1, then the packaging plate 17 is opened, the corresponding storage assembly 4 is pulled out through the pull groove 43, the sealing baffle plate 31 can be pushed under the elastic recovery action of the return spring 32 by the movement of the storage assembly 4 to move along with the storage assembly 4, and the interior of the incubator 2 is sealed again under the matching of the upper and lower adjacent sealing plates 42 and the pressing plate 9, at the moment, the pressing plate 9 at the top of the storage assembly 4 can push the positioning sleeve 8 and the movable block 11 to move downwards and press the positioning spring 12 to increase the elastic recovery performance, and meanwhile, the positioning sleeve 8 and the pressing plate 9 are exposed out of the culture dishes 7 so that an experimenter can grab;
the operation of the constant temperature system 13 can be controlled by the cooperation of the touch screen 23 and the controller, and the regulation and control mode of the environmental data is selected by the touch screen 23:
if the subjective mode is selected, the air pressure, humidity and temperature values to be regulated are sequentially input through the touch screen 23, and after the system is confirmed, corresponding equipment and related electromagnetic valves 137 are automatically opened after the input values are compared through the detection data of the sensor in the culture chamber 2;
if the objective mode is selected, the strains and the related conditions are selected through the touch screen 23, then the system automatically turns on the fan 131 according to the preset data in the controller, compares the preset data with the sensor inside the cultivation room 2, quickly determines the turned-on humidifier 133 or dryer 134 and refrigerator 135 or heater 136, and simultaneously turns on the electromagnetic valve 137 at the corresponding position, so as to quickly and automatically regulate and control the environment (temperature, humidity and air pressure) inside the cultivation room 2.

Claims (10)

1. The utility model provides an intelligent bacteria incubator, includes incubator (1) and cultivates room (2), its characterized in that: the culture device is characterized in that a plurality of storage assemblies (4) are inserted into the culture chamber (2), a blocking assembly (3) located on the back face of each storage assembly (4) is arranged inside the culture chamber (2), a storage groove (5) is formed in the top of each storage assembly (4), a plurality of uniformly-arranged positioning grooves (6) are formed in the bottom of the inner cavity of each storage groove (5), culture dishes (7) are placed inside each positioning groove (6), a positioning sleeve (8) is sleeved outside each culture dish (7), a pressing plate (9) is fixedly sleeved outside each positioning sleeve (8), and the total area of the plurality of pressing plates (9) is the same as the cross-sectional area of each storage groove (5);
the blocking assembly (3) comprises a blocking plate (31), the front surface of the blocking plate (31) is in contact with the storage assembly (4), the side surface of the blocking plate (31) is in contact with the culture chamber (2) or the pressing plate (9), the back surface of the blocking plate (31) is connected with a return spring (32), and the rear end of the return spring (32) is connected with the back surface of the inner cavity of the culture chamber (2);
deposit subassembly (4) including cultivateing frame (41), the front of cultivateing frame (41) is connected with closing plate (42), draw groove (43) have been seted up in the front of closing plate (42), ventilation groove (44) have been seted up to the bottom of cultivateing frame (41), the side of cultivateing frame (41) is connected with joint strip (45), joint strip (45) are pegged graft in the inside of incubator (1), cultivate the top and the contact of baffle (31) of frame (41) and closing plate (42).
2. The intelligent bacteria incubator of claim 1, wherein: the culture rack (41) is of an air-permeable rigid filter screen structure, and the sealing plate (42) is of a solid structure.
3. The intelligent bacteria incubator of claim 1, wherein: cultivate movable groove (10) of offering in constant head tank (6) bottom in frame (41), movable block (11) have been cup jointed to the inside in movable groove (10), the bottom of movable block (11) is connected with positioning spring (12), the bottom of positioning spring (12) is connected with the bottom of movable groove (10) inner chamber, the top and position sleeve (8) fixed connection of movable block (11), the internal diameter size of movable block (11) is the same with the internal diameter size of position sleeve (8).
4. The intelligent bacteria incubator of claim 1, wherein: the back of incubator (1) is provided with constant temperature system (13), two air inlet chamber (14) about the inside of cultivateing room (2) has been seted up, both sides all are connected with breather pipe (15) around air inlet chamber (14) inner chamber, breather pipe (15) communicate with constant temperature system (13) through the hose each other.
5. The intelligent bacteria incubator of claim 1, wherein: the inside grafting of cultivateing room (2) has and is located sealing plug (16) of depositing subassembly (4) front end, the back and sealing plate (42) contact of sealing plug (16), the front end of sealing plug (16) extends to outside and the fixedly connected with encapsulation board (17) of incubator (1), the back connection of encapsulation board (17) has sealing washer (18) that are located the sealing plug (16) outside, the back and the incubator (1) contact of sealing washer (18).
6. The intelligent bacteria incubator of claim 5, wherein: the left and right sides of incubator (1) has all seted up spout (19), every portion of spout (19) is connected with link (20) through the pivot, lead screw (21) have been cup jointed to the middle part screw thread of link (20), the back through link (20) and the joint of lead screw (21) have roof (22), roof (22) and encapsulation board (17) contact.
7. The intelligent bacteria incubator of claim 1, wherein: the top of incubator (1) is provided with touch-sensitive screen (23), the inside of incubator (1) is provided with the controller, touch-sensitive screen (23) pass through the wire and link to each other with the controller.
8. The intelligent bacteria incubator of claim 4, wherein: the air supply system is characterized in that a fan (131), an air supply pipe (132), a humidifier (133), a dryer (134), a refrigerator (135) and a heater (136) are arranged inside the constant temperature system (13), the air inlet end of the fan (131) is communicated with a part of the vent pipes (15) through a hose, the air outlet ends of the refrigerator (135) and the heater (136) are communicated with the other part of the vent pipes (15) through a three-way pipe, the air supply pipe (132) is communicated with the air inlet end of the fan (131), and the air inlet ends of the air supply pipe (132), the humidifier (133), the dryer (134), the refrigerator (135) and the heater (136) are provided with electromagnetic valves (137).
9. The intelligent bacteria incubator of claim 8, wherein: the air outlet end of the fan (131) is communicated with the air inlet ends of the humidifier (133) and the dryer (134) through a three-way pipe, and the air outlet ends of the humidifier (133) and the dryer (134) are communicated with the air inlet ends of the refrigerator (135) and the heater (136) through a four-way pipe.
10. An intelligent bacteria incubator and a use method thereof suitable for the intelligent bacteria incubator of claims 1-9 are characterized in that the operation method is as follows:
s1, integrally inserting a plurality of storage assemblies (4) for containing culture dishes and the culture dishes (7) into a culture chamber (2), and pushing a sealing baffle plate (31) to extrude a return spring (32) under the cooperation of the culture chamber (2) to increase the elastic recovery performance of the return spring;
s2, inserting a sealing plug (16) into the culture chamber (2) through a packaging plate (17), clamping a sealing ring (18) by the packaging plate (17) and the culture box (1), turning over a connecting frame (20) under the matching of a sliding groove (19) and a rotating shaft to enable the connecting frame to be turned over to the front end of the packaging plate (17), rotating a top plate (22) to enable the connecting frame (20) to push the packaging plate (17) through the top plate (22) under the matching of the connecting frame (20), and clamping the sealing ring (18) under the matching of the culture box (1), so that culture can be carried out;
s3, adjusting the internal environment of the culture chamber (2) through the cooperation of the touch screen (23) and the controller;
s4, if the subjective mode is selected through the touch screen (23), sequentially inputting air pressure, humidity and temperature values to be regulated through the touch screen (23), and automatically starting corresponding equipment and related electromagnetic valves (137) after confirming that the system compares the input values with the detection data of the sensor in the culture chamber (2);
s5, if an objective mode is selected, firstly, strains and relevant conditions are selected through the touch screen (23), then the system automatically starts the fan (131) according to preset data in the controller, compares the preset data with a sensor in the culture chamber (2), quickly judges the started humidifier (133) or drier (134) and refrigerator (135) or heater (136), and simultaneously starts the electromagnetic valve (137) at the corresponding position, so that the environment (temperature, humidity and air pressure) in the culture chamber (2) can be quickly and automatically regulated and controlled
S6, after culturing for a period of time, rotating the screw rod (21) to unlock the packaging plate (17) by the top plate (22), turning the connecting frame (20) to the upper part of the incubator (1) and opening the packaging plate (17); then the corresponding storage assembly (4) is pulled out through the pull groove (43), and the culture dish (7) of the corresponding part can be taken;
s7, the storage assembly (4) can move under the elastic force recovery action of the return spring (32) to push the sealing baffle plate (31) to move along with the storage assembly (4), and the interior of the culture chamber (2) is sealed again under the matching of the upper and lower adjacent sealing plates (42) and the pressing plate (9);
s8, press down this moment and take press down pressing plate (9) at culture frame (41) top and can promote position sleeve (8) and movable block (11) downstream and extrude positioning spring (12) and make its elasticity recovery performance increase, make position sleeve (8) and press pressing plate (9) to expose culture dish (7) so that the experimenter snatchs simultaneously, can take and use the bacterium that corresponds culture dish (7) inside.
CN202210823208.8A 2022-07-14 2022-07-14 Intelligent bacteria incubator and use method thereof Withdrawn CN115141711A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115786117A (en) * 2023-01-31 2023-03-14 山西正一康生物细胞存储有限公司 Biological stem cell storage culture reagent case

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115786117A (en) * 2023-01-31 2023-03-14 山西正一康生物细胞存储有限公司 Biological stem cell storage culture reagent case

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Application publication date: 20221004