CN114868539A - Intelligent step-by-step olive seedling growing method - Google Patents

Abstract

The invention belongs to the technical field of plant cutting propagation, in particular to an intelligent step-by-step method for raising olive seedlings. The invention provides an intelligent step-by-step method for raising olive oil seedlings for improving the quality, rooting rate and seedling emergence of olive oil cutting seedlings, shortening the seedling raising period and increasing the number of seedling raising. The method includes the following steps: selecting robust annual branches of olive oil as cuttings, soaking the cuttings in rooting liquid, and then cutting the treated cuttings in soil cuttings in an intelligently regulated greenhouse to raise seedlings; Control the temperature, light and humidity of cuttings before and after rooting. By adopting the method of the invention, the seedling raising period is shortened, the seedling raising frequency is increased, and the average rooting rate of the cuttings is increased by more than 50%.

Description

Intelligent step-by-step method for raising olive seedlings

technical field

The invention belongs to the technical field of plant cutting propagation, in particular to an intelligent step-by-step method for raising olive seedlings.

Background technique

Olive oil belongs to the Oleaceae Oleaceae evergreen tree, which is a world-famous oil plant and fruit tree species. Olive oil is native to countries along the Mediterranean coast, and the main cultivation countries are Spain, Italy, Greece and other countries. Olive oil has a history of domestication and cultivation for more than 6,000 years, and is an important economic crop and edible oil plant in the countries along the Mediterranean Sea. So far, more than 40 countries have introduced and cultivated olive oil.

my country introduced and cultivated olive oil on a large scale in 1964. So far, my country has introduced and cultivated olive oil for 57 years, and the planting area of olive oil has exceeded 1 million mu. The suitable areas for olive cultivation in my country include Xichang and Mianyang in Sichuan Province, Wudu in Gansu Province, Hanzhong in Shaanxi Province, Yongren and Lijiang in Yunnan Province, and Shiyan in Hubei Province, which have become the pillar industries for local farmers to get rich.

With the rapid development of the olive industry in my country, the planting area of olive oil has continued to expand, and the demand for olive seedlings has also increased rapidly. Continuing to ensure a sufficient supply of fine varieties is the premise and foundation for the healthy, stable and rapid development of the olive industry.

In order to obtain a better seedling effect, by selecting branches and cuttings with rooting agent, adjusting the greenhouse temperature, humidity, and light measures to obtain higher seedling emergence and root quality. People usually use cuttings to raise seedlings. However, the general steps for raising seedlings by cuttings of olive oil at present are: select the annual branches on the mother tree with general results, process the cuttings with rooting agents, and raise the cuttings in a greenhouse without any regulation facility (traditional seedling raising method). The method for raising seedlings by cuttings has a long seedling raising period and few seedling raising times on various varieties of olive oil, and can only raise seedlings twice a year. The temperature, humidity and light of the cuttings cannot be precisely controlled, and the cuttings cannot be provided with suitable environmental conditions for rooting, resulting in poor root quality, fewer roots, more rotten roots, and a low rooting rate, generally 20-40%. Seedlings are few.

Therefore, there is an urgent need to provide a new method suitable for oleifera cuttings to raise seedlings, thereby improving the quantity and quality of oleifera cuttings and raising seedlings, shortening the period of raising seedlings, and increasing the number of raising seedlings.

SUMMARY OF THE INVENTION

Aiming at the deficiencies of the existing method for raising seedlings by cuttings of olive oil, the present invention provides another method for raising seedlings by cuttings of olive oil, which solves the problems of long period of raising seedlings by cuttings of olive oil, less number of times of raising seedlings, poor root quality of cuttings, low rooting rate and seedling emergence. Minor technical issues.

The invention provides a new intelligent step-by-step method for raising olive seedlings for improving the quality, rooting rate and seedling emergence of olive oil cutting seedlings, shortening the seedling raising period and increasing the number of seedling raising. The method includes the following steps:

Select robust annual olive branches as cuttings, and the cuttings are soaked in rooting liquid, and then the processed cuttings are inserted into the soil cuttings in the intelligent control greenhouse to raise seedlings; temperature, light and humidity.

Among them, the olive branches are Crolika, Douguo, Colati, Ezhi No. 8, Laixing, Piguaer, Absana or Foao.

Among them, the time to collect shoots is selected in the morning, afternoon or cloudy day.

Among them, the thickness of the branches is 0.2-0.4 cm, and the length is not limited.

Among them, the cuttings are 10-12 cm long, and one pair of leaves is left at the upper end of the cuttings.

Wherein, the rooting agent is ABT1 rooting agent.

Wherein, the concentration of the rooting agent is 100ppm-200ppm.

Among them, the depth of the base of the soaking cuttings is 3-4 cm, and the cuttings are soaked for 4-6 hours.

Among them, the intelligent control greenhouse includes a cooling system, a heating system, a shading and supplementary light system, and a humidification and dehumidification system.

Further, the system senses the temperature, humidity and light in the greenhouse through multi-element sensors in the greenhouse.

Among them, before the cuttings take root, the light is controlled at 13-14 kilolux, the temperature is controlled at 23-24 degrees Celsius, and the air humidity is controlled at 80-90%.

Among them, after the cuttings are rooted, the light is controlled at 14-15 kilolux, the temperature is controlled at 23-24 degrees Celsius, and the air humidity is controlled at 65-75%.

Beneficial effects: compared with the traditional seedling raising, when the olive seedlings are cut and raised by the seedling raising method of the present invention, the seedling raising period is shortened by half, the annual seedling raising times is doubled, and the average rooting rate of the cuttings is improved by more than 50%. The invention strictly controls the temperature, humidity and light before and after the cuttings are rooted by intelligent steps. Before the cuttings are rooted, the light is controlled at 13-14 kilolux, the temperature is controlled at 23-24 degrees Celsius, and the air humidity is controlled at 80-90% . After the cuttings are rooted, the light is controlled at 14-15 kilolux, the temperature is controlled at 23-24 degrees Celsius, and the air humidity is controlled at 65-75%. Compared with other seedling raising methods that do not control the temperature, humidity and light before and after rooting of the cuttings within the scope of the present invention, the intelligent distributed seedling raising method of the present invention has no occurrence of defoliation, rot and disease, the number of root systems of the cuttings is excellent, and the average rooting rate is Highest. In addition, the intelligent step-by-step seedling raising method of the present invention saves labor costs and realizes industrialized production.

Detailed ways

Specific embodiments of the present invention will be described in detail below. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention, but not to limit the present invention.

The endpoints of ranges and any values disclosed herein are not limited to the precise ranges or values, which are to be understood to encompass values proximate to those ranges or values. For ranges of values, the endpoints of each range, the endpoints of each range and the individual point values, and the individual point values can be combined with each other to yield one or more new ranges of values that Ranges should be considered as specifically disclosed herein.

The present invention will be further described below in conjunction with specific embodiments.

The invention provides a new method for raising seedlings for improving the quality, rooting rate and seedling emergence of olive oil cuttings, shortening the seedling raising period and increasing the number of seedling raisings. The time to collect shoots is selected in the morning, afternoon or cloudy day. Select the robust annual branches on the mother tree of the picking garden. The thickness of the branches is 0.2-0.4 cm and the length is not limited. The collected branches are listed and placed in the cutting room to keep the branches fresh, and the branches are cut into qualified cuttings. The length of the strip is 10-12 cm, the upper end of the cutting is left with a pair of leaves, the lower end of the cutting is cut close to the position of the node, the upper end is cut at a distance of 0.2-0.4 cm from the node, and the cut is cut flat. Then soak the cuttings with rooting agent, use ABT1 rooting agent, use the concentration of 100ppm-200ppm, soak the cuttings to a depth of 3-4 cm at the base, and soak the cuttings for 4-6 hours. Finally, the treated cuttings are inserted into the plugs of the seedling trays in the intelligent control greenhouse for seedling cultivation. In the pre-rooting stage (30 days), the light is controlled at plus or minus 13.5 thousand lux (ie 13-14 thousand lux), the temperature is controlled at plus or minus 23.5 (ie 23-24 degrees Celsius) degrees Celsius, and the air humidity is controlled at plus or minus 85% (ie 80-90%). In the post-rooting stage (60 days), the light is controlled at plus or minus 14.5 thousand lux (ie 14-15 thousand lux), the temperature is controlled at plus or minus 23.5 degrees Celsius (ie 23-24 degrees Celsius), and the air humidity is controlled at plus or minus 70% (ie 65-75%). High-quality cuttings can be produced 90 days after cuttings.

The present invention adopts No. ABT1 rooting agent, using concentration 100ppm-200ppm, the purpose is that this rooting agent is used for rooting of olive cuttings, the number of rooting is large, the rooting time is short, and the rooting rate is high, and other rooting agents and concentrations can not achieve this effect. .

The model of the intelligent control greenhouse of the present invention is a glass greenhouse intelligent greenhouse, and the manufacturer is Qijiaming New Agricultural Development Co., Ltd. The intelligent control greenhouse is equipped with cooling system (fan and water curtain), heating system (boiler and hot water pipe), shading and supplementary light system (motor and shade net) humidification and dehumidification system (spray equipment and fan), these systems pass through the greenhouse The multi-element sensor inside senses the temperature, humidity, and light in the greenhouse, and then wirelessly transmits this information to the intelligent controller (smart control device or mobile phone), and performs intelligent step-by-step management and nursery according to the preset parameters.

The ear-picking garden of the present invention refers to an olive garden in which the main varieties with excellent performance are planted at a high density of 2m*3m or 2.5m*3m, providing high-quality branches for cutting and raising seedlings in greenhouses.

Main varieties of olive oil: Koroneika, Arbequina, Coratina, EZ-8, Leccino, Picual, Arbosana, Frantoio, etc.

Pre-rooting stage of cuttings: refers to the time period from the cuttings after cuttings to the growth of callus and no roots, which takes 30 days.

Post-rooting stage of cuttings: refers to the time period from the beginning of rooting to before emergence, which takes 90 days.

The solution of the present invention will be explained below in conjunction with the embodiments. Those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be construed as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, the technique or condition described in the literature in the field or the product specification is used. The reagents or instruments used without the manufacturer's indication are conventional products that can be obtained from the market.

In order to better illustrate the effect of the intelligent step-by-step method for raising seedlings of olive oil on olive cuttings and raising seedlings, the effect of shortening the seedling raising period and increasing the number of seedling raisings was compared. The situation of the seedling raising cycle and the seedling raising frequency of the invention.

Example 1 and Comparative Example 1: Comparison of Seedling Cycle and Seedling Times Adopting Two Kinds of Seedling Methods

The six main cultivars, Koroneika, Arbequina, Coratina, Leccino, Picual and EZ-8, were selected as annuals. For the branch cutting test, 500 cuttings of each variety were tested for the two seedling raising methods, and the branches were from the same mother tree. The cuttings of the two seedling raising methods are the same for cutting and retaining leaves. The treatment is treated with ABT No. 1 rooting agent with a concentration of 100 PPM, and the depth of the base of the cuttings is 3 cm-4 cm, and the cuttings are soaked for 4 hours. Seedling cycle (unit: day), seedling season, and annual number of seedlings (unit: times) for each variety, and the specific test results are shown in Table 1 below:

Table 1 Comparison of seedling cycle and seedling times using two seedling raising methods

It can be seen from the data of the above-mentioned comparative experiment 1 that the seedling raising method of the present invention can shorten the seedling raising period and increase the number of annual seedling raising.

Embodiment 2 and comparative example 2: adopt the situation of the rooting quality, rooting quantity (unit: strip) and average rooting rate (unit: %) of the seedling-raising cuttings of two seedling-raising methods, and the specific test results are as follows:

The main cultivars in the experiment were Koroneika, Arbequina, Coratina, EZ-8, and Picual. For the branch cutting test, 50 cuttings of each variety were tested for the two seedling raising methods, with 4 replicates, and the branches were from the same mother tree. The cuttings of the two seedling raising methods are the same for cutting and retaining leaves. The treatment is treated with ABT No. 1 rooting agent with a concentration of 100 PPM, and the depth of the base of the cuttings is 3 cm-4 cm, and the cuttings are soaked for 4 hours. The rooting quality, rooting quantity and average rooting rate of 5 varieties of the two seedling raising methods, the specific test results are shown in Table 2 below:

Root rot status of cuttings: rotted roots are indicated with yes, and no rotted roots are indicated with no. The number of root systems of cuttings: 1-5 is poor, 6-10 is good, and more than 10 is very good.

Table 2 The situation of rooting quality, rooting quantity (strip) and average rooting rate (%) of seedling-raising cuttings using two seedling-raising methods

It can be seen from the data of the above-mentioned comparative experiment 2 that the quality of the root system of the cuttings is improved by adopting the seedling raising method of the present invention, and the average rooting rate is increased by more than 50%.

Example 3 and Comparative Example 3: In the stage before rooting of cuttings in the intelligent step-by-step seedling raising method, different gradient illumination, temperature and relative air humidity were set up to compare the seedling raising method of the present invention. Five main cultivars, Koroneika, Arbequina, Coratina, EZ-8 and Picual, were selected for the experiment. The annual branch cuttings from the mother tree of 5 varieties were cut, and 50 cuttings of each variety were taken, with 4 repetitions, and the branches were from the same mother tree. The cuttings are cut the same, leaving 2 leaves uniformly. All treatments were treated with ABT No. 1 rooting agent with a concentration of 100 PPM, soaking the cuttings at a depth of 3 cm-4 cm at the base, and soaking the cuttings for 4 hours. The growth of cuttings of 5 varieties, whether there is disease, defoliation and rot (unit: strain) and the average number of callus produced (unit: strain), the specific test results are shown in Table 3 below:

Disease: Refers to the presence or absence of peacock spot disease on cutting leaves.

Defoliation: refers to the occurrence of 1 or 2 deciduous leaves on the cuttings.

Rot: Refers to the phloem at the base of the cuttings turning brown and black, with the smell of alcohol.

Temperature unit: Celsius, light unit: kilolux, air humidity unit: %RH

Table 3 In the stage before rooting of cuttings in the intelligent step-by-step method for raising seedlings, different gradient illumination, temperature and relative air humidity are set to compare test results with the method for raising seedlings of the present invention

From the data of the above-mentioned comparative experiment 3, it can be seen that in the stage before the cuttings take root, the method for raising seedlings of the present invention does not cause the cuttings to fall off, rot and susceptible to disease, and the cuttings produce the highest number of callus. It can be seen that in the pre-rooting stage of the cuttings in the intelligent step-by-step seedling raising method, any change of one of the test parameters does not bring about a better seedling raising effect than that of the method of the present invention.

Example 4 and Comparative Example 4: In the stage after the cuttings are rooted in the intelligent step-by-step seedling raising method, different gradient illumination, temperature and relative air humidity were set up to compare experiments with the seedling raising method of the present invention. Five main cultivars, Koroneika, Arbequina, Coratina, EZ-8 and Picual, were selected for the experiment. The annual branch cuttings on the mother tree of 5 varieties were cut, and 100 cuttings of each variety were taken, with 4 repetitions, and the branches were from the same mother tree. The cuttings are cut the same, leaving 2 leaves uniformly. All treatments were treated with ABT No. 1 rooting agent with a concentration of 100 PPM, soaking the cuttings at a depth of 3 cm-4 cm at the base, and soaking the cuttings for 4 hours. The rooting situation of cuttings of 5 varieties, the average number of root systems (unit: strip), the occurrence of disease, defoliation and rot (unit: plant), the number of cuttings rooted (unit: plant) and the average rooting rate (unit: plant) %), the specific test results are shown in Table 4 below:

Root rot of cuttings: Refers to the phloem at the base of the cuttings turning brown and black, with the smell of alcohol.

The number of root systems of cuttings: 1-5 is poor, 6-10 is good, and more than 10 is very good.

Disease: Refers to the presence or absence of peacock spot disease on cutting leaves.

Defoliation: refers to the occurrence of 1 or 2 deciduous leaves on the cuttings.

Table 4 In the stage after the rooting of the cuttings in the intelligent step-by-step seedling-raising method, different gradient illumination, temperature and relative air humidity are set and the comparative test results of the seedling-raising method of the present invention are set

It can be seen from the data of the above-mentioned comparative test 4 that in the stage after the rooting of the cuttings, the method for raising seedlings of the present invention does not cause the cuttings to fall off, rot and susceptible to disease, the cuttings have a particularly good root system, and the number of emerging seedlings and the average rooting rate are the highest. It can be seen that in the stage after the rooting of the cuttings in the intelligent step-by-step seedling raising method, if any one of the test parameters is changed, the effect of raising seedlings is not as good as that of the method of the present invention.

It can be seen from the above-mentioned 4 groups of comparative test data that when using the method for raising seedlings of the present invention to raise olive seedlings by cuttings, compared with traditional seedling raising, the seedling raising period is shortened by half, the number of annual seedling raising is doubled, and the average rooting rate of cuttings is improved by more than 50%. . Compared with other seedling raising methods of the intelligent step-by-step seedling raising method of the present invention, there is no occurrence of defoliation, rotting and disease susceptibility, the number of root systems of cuttings is particularly good, and the average rooting rate is the highest.

Claims (10)

1. The intelligent step-by-step olive seedling growing method is characterized by comprising the following steps: the method comprises the following steps:

selecting robust annual olive branches as cuttings, soaking the cuttings in a rooting solution, and then inserting the treated cuttings into cutting soil in an intelligent regulation and control greenhouse for seedling culture; wherein, the temperature, illumination and humidity before and after the cutting rooting are controlled step by step during seedling raising.

2. The intelligent step-by-step olive seedling growing method according to claim 1, characterized in that: the olive branches are Clelaca, Douglas, Korata, Ezhao No. 8, Luoxing, Pepper, Abusana or Foao.

3. The intelligent step-by-step olive seedling growing method according to claim 1 or 2, characterized in that: at least one of the following is satisfied:

selecting the time for collecting branches to be carried out in the morning, afternoon or cloudy day;

the thickness of the branch is selected to be 0.2-0.4 cm, and the length is not limited;

the length of the cutting is 10-12 cm, 1 pair of blades is left at the upper end of the cutting, the lower end of the cutting is cut close to the distance node, the distance node at the upper end is cut by 0.2-0.4 cm, and the cut opening is cut flat.

4. The intelligent step-by-step olive seedling growing method according to any one of claims 1-3, characterized in that: the rooting solution is ABT1 rooting agent.

5. The intelligent step-by-step olive seedling growing method according to any one of claims 1-4, characterized in that: the concentration of the rooting solution is 100ppm-200 ppm.

6. The intelligent step-by-step olive seedling growing method according to any one of claims 1-5, characterized in that: the depth of the base part of the cutting is 3-4 cm, and the cutting is soaked for 4-6 hours.

7. The intelligent step-by-step olive seedling growing method according to any one of claims 1-6, characterized in that: the intelligent regulation and control greenhouse comprises a cooling system, a heating system, a shading and light supplementing system and a humidifying and dehumidifying system.

8. The intelligent step-by-step olive seedling growing method according to claim 7, characterized in that: the system senses the temperature, humidity and illumination in the greenhouse through the multi-element sensor in the greenhouse.

9. The intelligent step-by-step olive seedling growing method according to any one of claims 1-8, characterized in that: before the cutting takes root, the illumination is controlled at 13-14 kilo-lux, the temperature is controlled at 23-24 ℃, and the air humidity is controlled at 80-90%.

10. The intelligent step-by-step olive seedling growing method according to any one of claims 1-9, characterized in that: after the cutting is rooted, the illumination is controlled at 14-15 kilo-lux, the temperature is controlled at 23-24 ℃, and the air humidity is controlled at 65-75%.