CN114839249A - Biosensor and precision improving method thereof - Google Patents
Biosensor and precision improving method thereof Download PDFInfo
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- CN114839249A CN114839249A CN202210640250.6A CN202210640250A CN114839249A CN 114839249 A CN114839249 A CN 114839249A CN 202210640250 A CN202210640250 A CN 202210640250A CN 114839249 A CN114839249 A CN 114839249A
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000008280 blood Substances 0.000 claims abstract description 180
- 210000004369 blood Anatomy 0.000 claims abstract description 180
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 42
- 239000008103 glucose Substances 0.000 claims abstract description 42
- 239000000758 substrate Substances 0.000 claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims abstract description 29
- 238000005259 measurement Methods 0.000 claims abstract description 12
- 230000001681 protective effect Effects 0.000 claims description 27
- 235000012054 meals Nutrition 0.000 claims description 15
- 230000000291 postprandial effect Effects 0.000 claims description 13
- 239000011888 foil Substances 0.000 claims description 10
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 9
- 229910052782 aluminium Inorganic materials 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
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- 230000003203 everyday effect Effects 0.000 claims description 6
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- 229940116332 glucose oxidase Drugs 0.000 description 3
- 235000019420 glucose oxidase Nutrition 0.000 description 3
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1418—Depression, aspiration
- G01N2001/1427—Positive displacement, piston, peristaltic
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Abstract
The invention belongs to the technical field of biosensors, and particularly relates to a biosensor and a precision improving method thereof, wherein the precision improving method comprises the following steps: the device comprises a substrate, a reaction layer, an electrode plate, a collecting pipeline and a piston; the piston is connected in the collection pipeline in a sliding and sealing mode, one side of the piston is fixedly connected with the sliding rod, and the problem that the operation of patients with hyperglycemia is not standardized sometimes, so that the test paper cannot absorb sufficient blood, the blood volume is insufficient, the deviation rate is up to 39% -85%, the blood volume is insufficient, the blood glucose meter can report errors sometimes, blood sampling measurement needs to be carried out again, and the test paper is a disposable product, so that waste is caused is solved; according to the invention, the piston and the collection pipeline are arranged for collecting blood, whether the collected blood is sufficient is judged by observing the blood volume of the collection pipeline, so that sufficient blood volume is ensured to enter the reaction layer, the accuracy of blood sugar detection is ensured, blood can be enriched when the blood volume is insufficient, and test paper does not need to be wasted.
Description
Technical Field
The invention belongs to the technical field of biosensors, and particularly relates to a biosensor and a precision improving method thereof.
Background
A biosensor is an instrument that is sensitive to a biological substance and converts its concentration into an electrical signal for detection; is an analysis tool or system which is composed of immobilized biological sensitive materials as recognition elements (comprising biological active substances such as enzyme, antibody, antigen, microorganism, cell, tissue, nucleic acid and the like), a proper physicochemical transducer (such as an oxygen electrode, a photosensitive tube, a field effect tube, a piezoelectric crystal and the like) and a signal amplification device; the biosensor has the functions of a receptor and a transducer.
When monitoring self blood sugar, patients with hyperglycemia also need to detect components such as cells, microorganisms, enzymes and the like in vivo, and when monitoring self blood sugar, hyperglycemia patients mostly use glucometers which measure blood sugar by measuring electric current generated by reaction of glucose in blood and glucose oxidase or glucose dehydrogenase in test paper, wherein when sampling blood, test paper is mostly used as a sampling means;
the test paper is used as one of the biosensors; in order to monitor blood sugar, most of patients with hyperglycemia have portable blood glucose meters for blood sugar monitoring, but the operation of the patients is not standard sometimes, so that the test paper cannot absorb sufficient blood, the blood volume is insufficient, the deviation rate is up to 39% -85%, the blood volume is insufficient, the blood glucose meters can report errors sometimes, blood sampling measurement needs to be carried out again, and the test paper is a disposable product, so that waste is caused.
In view of this, the present invention provides a biosensor and a method for improving the accuracy thereof, which solve the above technical problems.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a biosensor and a precision improving method thereof; the problem of hyperglycemia patient can carry out blood glucose measurement by oneself, but these patients 'operation is sometimes not standard, causes the test paper can't absorb sufficient blood, and the blood volume is not enough, and the deviation rate reaches 39% -85%, and the blood volume is not enough still can make the blood glucose meter report the mistake sometimes, need to take a blood sample again and measure, and the test paper is disposable, therefore causes the waste is solved.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention provides a biosensor, comprising:
a substrate;
the reaction layer is fixed inside the substrate;
the electrode plate is fixedly connected to one end of the outer side of the substrate and is electrically connected with the reaction layer;
the collecting pipeline is arranged at one end of the substrate, which is far away from the electrode plate, and is communicated with the reaction layer; the collection pipeline is used for collecting blood.
The piston is connected in the collecting pipeline in a sliding and sealing mode, and a sliding rod is fixedly connected to one side of the piston.
Preferably, the piston is fixedly connected with a blood taking needle at one side far away from the sliding rod.
Preferably, the outer side of the piston is sleeved with a rubber ring or a sealing element for increasing friction force.
Preferably, a protective cover is fixedly connected to one end, close to the liquid inlet of the collecting pipeline, of the substrate.
Preferably, the protective cover is made of transparent materials; so that the blood sampling condition can be conveniently observed through the protective cover.
Preferably, the protective cover is horn-shaped, the smaller end of the protective cover is fixedly connected with the substrate, and the protective cover is made of flexible materials.
Preferably, the larger end of the protective cover is fixedly connected with aluminum foil paper.
Preferably, the substrate is fixedly connected with a cover plate on the collecting pipeline, and the cover plate is made of transparent materials.
Preferably, the cover plate is provided with scale marks and can be used as a measuring standard.
The invention relates to a method for improving the accuracy of a biosensor, which is suitable for the biosensor and comprises the following steps:
s1, firstly, wiping and disinfecting the fingertip to be sampled with alcohol;
s2, after drying the alcohol in S1, pinching the substrate with a hand, removing the aluminum foil paper, aligning the protective cover with the puncture position, sliding the slide bar, puncturing the finger, reversely sliding the slide bar, and sucking the blood at the puncture position into the collection pipeline;
s3, after sufficient blood is collected in the collecting tube in S2, inserting the electrode plate of the substrate into a glucometer, detecting the current change of the reaction layer by the glucometer, and reading and displaying a blood glucose value;
s4, recording the blood sugar value measured in S3 by a blood glucose meter, determining the state of the patient as a fasting state, 1 hour after a meal, 2 hours after a meal or 3 hours after a meal during measurement, and storing the measured blood sugar value into a corresponding storage space;
s5, recording the data measured by fasting every day in S4 as X 1 ,X 2 ，……，X n Comparing X0 measured on empty stomach of the day with the period of week, month, quarter and year, and calculating the difference DeltaX ═ X 0 -X n L, obtaining differences Δ X in cycles of week, month, quarter and year, respectively w 、ΔX m 、ΔX q 、ΔX y ；
S6, recording the data measured after 1 hour every day in S4 as A 1 ，A 2 ，……，A n (ii) a Data measured 2 hours after each meal are recorded as B 1 ,B 2 ，……，B n (ii) a Data measured 3 hours after each meal are recorded as C 1 ,C 2 ，……，C n (ii) a And drawing a postprandial blood glucose curve F 1 (x)，F 2 (x) … … Fn (X), according to X measured the day 0 、A 0 、B 0 、C 0 And drawing a postprandial blood glucose curve F 0 (x) And comparing the measured values with the period of week, month, quarter and year to calculate the deviation ratio delta
Obtaining deviation ratios delta Y of cycles of week, month, quarter and year respectively w 、ΔY m 、ΔY q 、ΔY y ；
S7, comparing whether the data delta X or delta Y in the S5 and the S6 exceed a threshold value or not, alarming and prompting the blood glucose meter after the data delta X or delta Y exceed the threshold value, reminding the patient to measure the blood glucose again for calibration, and recording the abnormal value if the deviation of the blood glucose is still large, reminding the patient to control the blood glucose and seek medical advice in time.
The invention has the following beneficial effects:
1. according to the biosensor and the precision improvement method thereof, the piston and the collection pipeline are arranged for collecting blood, whether the collected blood is sufficient or not is judged by observing the blood volume of the collection pipeline, so that sufficient blood volume is ensured to enter the reaction layer, the accuracy of blood sugar detection is ensured, blood can be enriched when the blood volume is insufficient, and no test paper is wasted.
2. According to the biosensor and the precision improvement method thereof, the rubber ring is sleeved on the outer side of the piston, so that the sealing performance between the piston and the collection pipeline is improved, the piston and the collection pipeline have good sealing performance during blood collection, the piston has enough pressure when sliding in the collection pipeline, blood is convenient to be drawn into the collection pipeline, the friction force between the piston and the collection pipeline is increased by the rubber ring, and the sliding speed is convenient to control during sliding.
3. According to the biosensor and the precision improvement method thereof, the protective cover is arranged, when blood sampling is carried out, after fingers are disinfected, the protective cover covers a blood sampling part, the sliding rod is slid again to drive the blood sampling needle to puncture the fingers, blood sampling operation is carried out, and at the moment, blood is isolated from outside air, so that the influence of temperature and humidity on blood sugar detection is reduced, the test paper adopting glucose oxidase is not easily influenced by oxygen in the air, and the accuracy of blood sugar detection is improved.
4. According to the biosensor and the precision improving method thereof, the aluminum foil paper is arranged and fixedly connected with the protective cover through heat sealing or adhesive, before the biosensor is used, the protective cover is sealed by the aluminum foil paper, so that blood taking needles and the like are isolated from air, test paper is effectively protected, when the biosensor is used, the aluminum foil paper is removed, blood is taken again, the blood taking needles are protected from being oxidized, tetanus cannot be easily caused during blood taking, rubber rings are protected, oxidation and aging cannot be easily caused, the sealing performance is guaranteed, the reaction layer is protected, and the detection accuracy is guaranteed.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a perspective view of a biosensor according to the present invention;
FIG. 2 is a perspective view of the cover plate of the present invention when hidden;
FIG. 3 is an internal structural view of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 3 at A;
FIG. 5 is a diagram of the method steps of the present invention;
FIG. 6 is a graph of the postprandial blood glucose of person # 1 recorded by the present invention;
FIG. 7 is a post-prandial blood glucose graph of person # 2 recorded by the present invention;
FIG. 8 is a post-prandial blood glucose graph for person # 3 recorded by the present invention;
FIG. 9 is a post-prandial blood glucose graph for person # 4 recorded by the present invention;
FIG. 10 is a post-prandial blood glucose graph for person # 5 recorded by the present invention;
FIG. 11 is a post-prandial blood glucose graph of person number 6 recorded by the present invention;
in the figure: 1. a substrate; 11. a reaction layer; 12. an electrode sheet; 13. collecting a pipeline; 14. a liquid inlet; 2. a piston; 21. a slide bar; 22. a rubber ring; 3. a blood collection needle; 4. a protective cover; 5. aluminum foil paper; 6. a cover plate; 61. scale lines are marked.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
The embodiment of the invention provides a biosensor and a precision improving method thereof, and solves the technical problems that hyperglycemia patients can measure blood sugar by themselves, but the operation of the patients is sometimes not standard, so that test paper cannot absorb sufficient blood, the blood volume is insufficient, the deviation rate is as high as 39% -85%, the blood volume is insufficient, a blood sugar meter can report errors sometimes, blood sampling measurement needs to be carried out again, and the test paper is a disposable product, so that waste is caused;
in order to solve the technical problems, the technical scheme in the embodiment of the invention has the following general idea: carry out the blood sampling through setting up piston 2 and collection pipeline 13, through observing the blood volume of collection pipeline 13 and judging whether the sufficient of blood sampling, ensure that there is sufficient blood volume to get into reaction layer 11, guarantee blood sugar detection's accuracy, and can enrich the blood when the blood volume is not enough, need not extravagant test paper.
As shown in fig. 1 to 11, a biosensor according to the present invention includes:
a substrate 1;
the reaction layer 11, the said reaction layer 11 is fixed inside said substrate 1;
the electrode plate 12 is fixedly connected to one end of the outer side of the substrate 1, and the electrode plate 12 is electrically connected with the reaction layer 11;
the collecting pipeline 13 is arranged at one end of the substrate 1 far away from the electrode plate 12, and the collecting pipeline 13 is communicated with the reaction layer 11;
the piston 2 is connected in the collecting pipeline 13 in a sliding and sealing mode, and one side of the piston 2 is fixedly connected with a sliding rod 21;
hyperglycemia patients can measure blood sugar by themselves, but the operation of the patients is not standard sometimes, so that the test paper cannot absorb sufficient blood, the blood volume is insufficient, the deviation rate is up to 39% -85%, the blood volume is insufficient, the blood sugar meter can report errors sometimes, blood sampling measurement needs to be carried out again, and the test paper is a disposable product, so that waste is caused;
in the invention, by arranging the piston 2, when measuring blood sugar, firstly, the fingertip is wiped and disinfected by alcohol, the fingertip is punctured by a sharp object after the alcohol is dried, the substrate 1 is pinched by hands, the collection pipeline 13 is aligned to the punctured part, a slide bar 21 is slid by a finger, so that the piston 2 slides in the collection pipeline 13, the fingertip blood at the punctured part is sucked into the collection pipeline 13, at the moment, the piston 2 blocks the blood from entering the reaction layer 11, after the slide bar 21 is pulled for a certain distance, the blood volume is observed from the liquid inlet 14 of the collection pipeline 13, if the blood volume at the liquid inlet 14 is full, the blood collection volume is sufficient, if the blood volume at the liquid inlet 14 is insufficient, the blood collection volume is insufficient, at the moment, the slide bar 21 is reversely slid, the blood is pushed to the liquid inlet 14, the blood is continuously sucked until the blood volume in the collection pipeline 13 is sufficient, and the slide bar 21 is continuously slid after the blood volume in the collection pipeline 13 is full, the piston 2 is made to continuously slide to one end of the collection pipeline 13 far away from the liquid inlet 14, at the moment, the collection pipeline 13 is communicated with the reaction layer 11, under the action of capillary effect, blood in the collection pipeline 13 enters the reaction layer 11 to react with enzyme in the reaction layer 11, the electrode plate 12 is inserted into a blood glucose meter, and the blood glucose meter detects the current change of the reaction layer 11 and displays the value of blood glucose;
in the invention, the piston 2 and the collection pipeline 13 are arranged for collecting blood, whether the collected blood is sufficient is judged by observing the blood volume of the collection pipeline 13, so that the sufficient blood volume is ensured to enter the reaction layer 11, the accuracy of blood sugar detection is ensured, blood can be enriched when the blood volume is insufficient, a biosensor does not need to be replaced, and the waste is reduced.
As shown in fig. 3 and 4, the piston 2 is fixedly connected with the blood taking needle 3 at the side far away from the slide bar 21;
through setting up blood taking needle 3, under the initial condition, piston 2 is in collection pipeline 13 depths, piston 2 keeps away from collection pipeline 13's inlet 14 this moment, blood taking needle 3 hides in collection pipeline 13 this moment, after disinfecting the finger, slide bar 21 slides, promote piston 2 and outwards remove, thereby make blood taking needle 3 expose, stab to the collection after the disinfection, prick finger skin, make there is blood to flow out, slide bar 21 slides again, make piston 2 remove to collection pipeline 13, carry out the blood sampling operation, blood taking needle 3 hides in collection pipeline 13 this moment, through with blood taking needle 3 integration on piston 2, and hide blood taking needle 3 in collection pipeline 13, and convenient to use, and after the use, be difficult for hindering the people, blood infection is difficult to take place, the security of using is improved.
As shown in fig. 4, a rubber ring 22 is sleeved outside the piston 2;
through establish rubber ring 22 at piston 2 outside cover, improve the leakproofness of piston 2 and collection pipeline 13, when taking blood, good leakproofness between piston 2 and the collection pipeline 13, make piston 2 when collection pipeline 13 slides, sufficient pressure has, thereby be convenient for extract collection pipeline 13 with blood, and rubber ring 22 has increased the frictional force between piston 2 and the collection pipeline 13, when sliding, be convenient for control slip speed, prevent to slide too fast, it is darker to be difficult for making blood taking needle 3 pierce the degree of depth, prevent to lead to the fact great injury to the finger, and be difficult for making piston 2 remove too fast when extracting blood and make collection pipeline 13 and reaction layer 11 intercommunication, thereby be convenient for observe the amount of blood in the collection pipeline 13.
As shown in fig. 1 to 3, a protective cover 4 is fixedly connected to one end of the substrate 1 close to the liquid inlet 14 of the collecting pipeline 13;
through setting up protection casing 4, when taking a blood sample, accomplish the back to the finger disinfection, cover the blood sampling position with protection casing 4, slide bar 21 again slides, and drive blood taking needle 3 punctures the finger, carries out the blood sampling operation, and blood and outside air are isolated this moment to reduce the influence of temperature and humidity to blood sugar detection, and to the test paper that adopts glucose oxidase, be difficult for receiving the influence of the oxygen in the air, improve blood sugar detection's accuracy.
As shown in fig. 1 to 3, the protective cover 4 is made of a transparent material;
through adopting transparent material preparation antiskid cover, be convenient for observe the puncture state and the amount of bleeding of finger, ensure to gather the blood of sufficient, guarantee the accuracy that detects, transparent material can use plastics, resin etc..
The protective cover 4 is horn-shaped, the smaller end of the protective cover 4 is fixedly connected with the substrate 1, and the protective cover 4 is made of flexible materials;
through setting up protection casing 4 to loudspeaker form, and adopt the flexible material preparation, when taking a blood sample, be convenient for adsorb protection casing 4 on the finger, further isolated air, and strengthen the test paper and be connected with the finger, the difficult dislocation that takes place guarantees the stability of blood sampling process, convenient to operate and use.
As shown in fig. 3, the larger end of the protective cover 4 is fixedly connected with an aluminum foil 5;
through setting up aluminium foil paper 5, aluminium foil paper 5 links firmly with protection casing 4 through heat seal or viscose, before the use, aluminium foil paper 5 seals protection casing 4 for blood taking needle 3 etc. are isolated with the air, effective protection test paper, when using, take off aluminium foil paper 5, take a blood sample again, 3 difficult oxidations of protection blood taking needle, be difficult for causing the tetanus when the blood sampling, protection rubber circle 22, difficult oxidation is ageing, guarantee the leakproofness, protection reaction layer 11, guarantee the accuracy that detects.
As shown in fig. 1, 3 and 4, the substrate 1 is fixedly connected with a cover plate 6 on the collecting pipeline 13, and the cover plate 6 is made of a transparent material;
the cover plate 6 is provided with scale marks 61;
through setting up transparent apron 6, be convenient for observe the blood volume in the collection pipeline 13, and set up scale mark 61 on apron 6, be convenient for the blood volume in the comparatively accurate definite collection pipeline 13, thereby confirm the blood volume, guarantee can gather the blood of capacity, and can observe the blood situation in the collection pipeline 13, when there are more bubbles, release blood, blood is again collected, guarantee the accuracy of blood collection, when pricking the finger, can control the needle insertion degree of depth, it is too dark and cause the excessive hemorrhage to reduce the needle insertion, it is too shallow and the blood collection is not enough to reduce the needle insertion, make blood taking needle 3 pierce comparatively suitable degree of depth, guarantee the blood volume, reduce the injury to the finger.
The invention relates to a method for improving the accuracy of a biosensor, which is suitable for the biosensor and comprises the following steps:
s1, firstly, wiping and disinfecting the fingertip to be sampled with alcohol;
s2, after drying the alcohol in S1, pinching the substrate 1 by hand, uncovering the aluminum-foil paper 5, aligning the protective cover 4 to the punctured position, sliding the slide bar 21, puncturing the finger, reversely sliding the slide bar 21, and sucking the blood at the punctured position into the collection pipeline 13;
s3, after sufficient blood is collected in the collecting tube in S2, the electrode sheet 12 of the substrate 1 is inserted into a glucometer, and the glucometer detects the current change of the reaction layer 11 and reads and displays the blood glucose value;
s4, recording the blood sugar value measured in S3 by a blood glucose meter, determining the state of the patient as a fasting state, 1 hour after a meal, 2 hours after a meal or 3 hours after a meal during measurement, and storing the measured blood sugar value into a corresponding storage space;
s5, recording the data measured by fasting every day in S4Is X 1 ,X 2 ，……，X n Comparing X0 measured on empty stomach of the day with the period of week, month, quarter and year, and calculating the difference DeltaX ═ X 0 -X n L, obtaining differences Δ X in cycles of week, month, quarter and year, respectively w 、ΔX m 、ΔX q 、ΔX y ；
S6, recording the data measured after 1 hour every day in S4 as A 1 ，A 2 ，……，A n (ii) a Data measured 2 hours after each meal are recorded as B 1 ,B 2 ，……，B n (ii) a Data measured 3 hours after each meal are recorded as C 1 ,C 2 ，……，C n (ii) a And drawing a postprandial blood glucose curve F 1 (x)，F 2 (x) … … Fn (X), according to X measured the day 0 、A 0 、B 0 、C 0 And drawing a postprandial blood glucose curve F 0 (x) And comparing the measured values with the period of week, month, quarter and year to calculate the deviation ratio delta
Obtaining deviation ratios delta Y of cycles of week, month, quarter and year respectively w 、ΔY m 、ΔY q 、ΔY y ；
S7, comparing whether the data delta X or delta Y in the S5 and the S6 exceed a threshold value or not, alarming and prompting the blood glucose meter after the data delta X or delta Y exceed the threshold value, reminding the patient to measure the blood glucose again for calibration, and recording the abnormal value if the deviation of the blood glucose is still large, reminding the patient to control the blood glucose and seek medical advice in time.
The data recorded in the practical use of the invention are as follows, the blood sugar measurement results of 6 persons are selected and respectively numbered as 1, 2, 3, 4, 5 and 6, wherein 1, 2 and 3 are persons with normal blood sugar, 4, 5 and 6 are hyperglycemia patients, and the table 1 shows the blood sugar values of the 6 persons measured in different time periods;
table 1: blood sugar value of 6 persons in different time periods
Calculating the blood glucose difference value delta X and the deviation rate delta Y of 6 persons in different time periods according to the data in the table 1, drawing a postprandial blood glucose curve of 6 persons in different time periods, as shown in fig. 6 to 11, and calculating results as shown in table 2;
table 2: blood glucose difference value delta X and deviation rate delta Y of 6 persons in different time periods
According to the curves in fig. 6 to fig. 11 and the calculation results in table 2, the blood sugar fluctuation of the person to be tested at different time intervals can be found, the blood sugar difference value delta X is less than or equal to 2, delta Y is less than or equal to 15, the blood sugar difference value delta X is normal, the blood sugar difference value delta Y is set according to the requirements of doctors, the blood sugar difference values of No. 4 and No. 6 can be found to have larger fluctuation in a short time, the blood sugar meter can give out warning sound to remind the patient to re-measure the blood sugar, whether the blood sugar is measured by mistake or not is determined, the measurement precision is ensured, after re-measurement, if the fluctuation is smaller, the measurement result is recorded, and if the fluctuation is still larger, the patient is reminded to control the blood sugar and get medical advice in time.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A biosensor, comprising:
a substrate (1);
a reaction layer (11), wherein the reaction layer (11) is fixed inside the substrate (1);
the electrode plate (12), the electrode plate (12) is fixedly connected with one end of the outer side of the substrate (1), and the electrode plate (12) is electrically connected with the reaction layer (11);
the collecting pipeline (13) is arranged at one end, far away from the electrode plate (12), of the substrate (1), and the collecting pipeline (13) is communicated with the reaction layer (11);
piston (2), piston (2) sliding seal connects in gathering pipeline (13), piston (2) one side links firmly slide bar (21).
2. A biosensor as claimed in claim 1, wherein: the piston (2) is fixedly connected with a blood taking needle (3) at one side far away from the slide bar (21).
3. A biosensor as claimed in claim 2, wherein: and a rubber ring (22) is sleeved on the outer side of the piston (2).
4. A biosensor as claimed in claim 3, wherein: the substrate (1) is fixedly connected with a protective cover (4) at one end close to the liquid inlet (14) of the collecting pipeline (13).
5. A biosensor as claimed in claim 4, wherein: the protective cover (4) is made of transparent materials.
6. A biosensor as claimed in claim 5, wherein: the protective cover (4) is horn-shaped, the smaller end of the protective cover (4) is fixedly connected with the substrate (1), and the protective cover (4) is made of flexible materials.
7. A biosensor as claimed in claim 6, wherein: the larger end of the protective cover (4) is fixedly connected with aluminum foil paper (5).
8. A biosensor as claimed in claim 7, wherein: the substrate (1) is in acquisition pipeline (13) is last to be linked firmly apron (6), apron (6) are transparent material.
9. A biosensor as claimed in claim 8, wherein: and the cover plate (6) is provided with scale marks (61).
10. A biosensor precision improving method is characterized in that: the method is applied to the biosensor of any one of claims 1-9, and comprises the following steps:
s1, firstly, wiping and disinfecting the fingertips to be sampled with alcohol;
s2, after drying the alcohol in S1, pinching the substrate (1) by hand, uncovering the aluminum foil paper (5), aligning the protective cover (4) to the puncture position, sliding the sliding rod (21), puncturing the finger, reversely sliding the sliding rod (21), and sucking the blood at the puncture position into the collection pipeline (13);
s3, after sufficient blood is collected in the collection tube in S2, the electrode plate (12) of the substrate (1) is inserted into a glucometer, and the glucometer detects the current change of the reaction layer (11) and reads and displays the blood glucose value;
s4, recording the blood sugar value measured in S3 by a blood glucose meter, determining the state of the patient as a fasting state, 1 hour after a meal, 2 hours after a meal or 3 hours after a meal during measurement, and storing the measured blood sugar value into a corresponding storage space;
s5, recording the data measured by fasting every day in S4 as X 1 ,X 2 ，……，X n Comparing X0 measured on empty stomach of the day with the period of week, month, quarter and year, and calculating the difference DeltaX ═ X 0 -X n L, obtaining differences Δ X in cycles of week, month, quarter and year, respectively w 、ΔX m 、ΔX q 、ΔX y ；
S6, recording the data measured after 1 hour every day in S4 as A 1 ，A 2 ，……，A n (ii) a Data measured 2 hours after each meal are recorded as B 1 ,B 2 ，……，B n (ii) a Data measured 3 hours after each meal are recorded as C 1 ,C 2 ，……，C n (ii) a And drawing a postprandial blood glucose curve F 1 (x)，F 2 (x)，……F n (x) According to the day's measured X 0 、A 0 、B 0 、C 0 And drawing a postprandial blood glucose curve F 0 (x) And comparing the measured values with the period of week, month, quarter and year to calculate the deviation ratio delta
Obtaining deviation ratios delta Y of cycles of week, month, quarter and year respectively w 、ΔY m 、ΔY q 、ΔY y ；
S7, comparing whether the data delta X or delta Y in the S5 and the S6 exceed a threshold value or not, alarming and prompting the blood glucose meter after the data delta X or delta Y exceed the threshold value, reminding the patient to measure the blood glucose again for calibration, and recording the abnormal value if the deviation of the blood glucose is still large, reminding the patient to control the blood glucose and seek medical advice in time.
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