CN114767779A

CN114767779A - Biological preparation capable of repairing and inhibiting cervical erosion and HPV and preparation method thereof

Info

Publication number: CN114767779A
Application number: CN202210478448.9A
Authority: CN
Inventors: 武宽洪
Original assignee: Jiangsu Hongmaotang Pharmaceutical Trade Co ltd
Current assignee: Jiangsu Hongmaotang Pharmaceutical Trade Co ltd
Priority date: 2022-05-05
Filing date: 2022-05-05
Publication date: 2022-07-22

Abstract

The invention belongs to the technical field of biological preparations, and particularly relates to a traditional Chinese medicine biological preparation with a repairing and inhibiting effect on cervical erosion and HPV (human papilloma virus) and a preparation method thereof, wherein the traditional Chinese medicine biological preparation comprises the following raw materials in parts by weight: 28-35 parts of wild buckwheat rhizome, 25-31 parts of cape jasmine fruit, 22-30 parts of gynostemma pentaphylla, 18-25 parts of dogwood, 15-25 parts of hairyvein agrimony, 15-20 parts of burdock, 10-15 parts of aloe, 0.5-1.5 parts of yeast beta-glucan, 0.2-0.5 part of honey and 0.015-0.03 part of borneol. The preparation method is simple, the cost is low, the adopted raw materials are all effective extraction components in common Chinese medicinal materials, the raw materials directly act on the focus, the first pass effect of the liver is avoided, the test shows that the traditional Chinese medicine has better repairing and inhibiting effects on cervical erosion and HPV infection, has good inhibiting effect on gynecological inflammation caused by other various reasons, has definite curative effect, is convenient to administer, safe, free of stimulation, good in stability and high in patient compliance, and is suitable for batch large-scale production.

Description

Biological preparation capable of repairing and inhibiting cervical erosion and HPV and preparation method thereof

Technical Field

The invention belongs to the technical field of biological preparations, and particularly relates to a traditional Chinese medicine biological preparation with a repairing and inhibiting effect on cervical erosion and HPV, and a preparation method and application thereof.

Background

Cervical erosion is one of the local features of chronic cervicitis, is the most common gynecological disease of female reproductive system, has incidence rate of about 85% in women in the fertility stage, and shows gynecological inflammation due to physical, chemical and biological factors. Both of these so-called "cervical erosion" appearances, whether physiological or so-called pathological, require treatment by external therapy as long as they have symptoms such as leukorrhagia, lumbago, and after-bleeding in the same room. If symptomatic, even physiological changes are present, treatment may sometimes be considered. If the cervical cancer is a precancerous lesion or an early cervical cancer, the diagnosis can be carried out only by combining cervical cytology examination, HPV virus examination and the like. Therefore, in the case of cervical erosion, the health and daily life of women are seriously affected, and if the treatment is not carried out in time, the cervical cancer may be transformed. So it can neither exaggerate nor ignore cervical erosion.

The clinical treatment of cervical erosion is common in operations, such as microwave, laser, freezing and the like, and the conization can also be performed. The effect is certainly different from the type of operation. If microwave, laser and freezing are carried out, the later fertility is easily affected, so the treatment mode is more suitable for women without fertility requirements and is not suitable for women in all ages.

HPV is an abbreviation for human papillomavirus, which is capable of causing squamous epithelial proliferation of human skin mucosa. Infection in HPV patients is often evident as a localized lesion with the following symptoms: lumbago, abnormal leucorrhea, leukorrhagia, vaginal irregular bleeding, and severe infection manifested by verruca vulgaris, condyloma acuminatum, etc. HPV positivity, especially high-risk HPV positivity, has no exact curative effect treatment mode at the present stage, and continuous high-risk HPV positivity is a main reason for inducing female cervical cancer. The traditional Chinese medicine researches believe that the virus infection is mainly caused by invasion of exogenous pathogenic factors, damp-heat toxin stasis and failure of the body resistance to surpass the pathogenic factors.

In recent years, with the pace of life of people increasing, the incidence of various gynecological diseases increases year by year and tends to be younger and more advanced, and if the gynecological diseases are not treated in time, the gynecological diseases are not cured for a long time, and the life and the work of patients are seriously affected. Therefore, the significance of prevention and control of HPV virus on female health is taken as important importance, the main treatment is to detoxify and improve the immunity of the organism, and meanwhile, the medicament can quickly and effectively enter targeted cells and targeted tissues to directly reach focuses, block HPV infection, eliminate HPV virus and prevent cervical canceration.

However, the existing related medicines usually take western medicines as main medicines, the preparation process is quite complex, the side effects are obvious, the adaptability of patients is poor, and certain potential safety hazards exist.

Disclosure of Invention

In order to achieve the purpose, the invention discloses a traditional Chinese medicine biological preparation with a repairing and inhibiting effect on cervical erosion and HPV, a preparation method and application thereof, and solves the technical problems in the background technology.

The technical scheme adopted by the invention is as follows: a traditional Chinese medicine biological preparation having a repairing and inhibiting effect on cervical erosion and HPV comprises the following raw materials in parts by weight: 28-35 parts of wild buckwheat rhizome, 25-31 parts of cape jasmine fruit, 22-30 parts of gynostemma pentaphylla, 18-25 parts of dogwood, 15-25 parts of hairyvein agrimony, 15-20 parts of burdock, 10-15 parts of aloe, 0.5-1.5 parts of yeast beta-glucan, 0.2-0.5 part of honey and 0.015-0.03 part of borneol.

Further, the feed comprises the following raw materials in parts by weight: 28 parts of wild buckwheat rhizome, 25 parts of cape jasmine fruit, 22 parts of gynostemma pentaphylla, 18 parts of dogwood, 15 parts of hairyvein agrimony, 15 parts of great burdock achene, 10 parts of aloe, 0.5 part of yeast beta-glucan, 0.2 part of honey and 0.015 part of borneol.

Further, the feed comprises the following raw materials in parts by weight: 30 parts of wild buckwheat rhizome, 28 parts of cape jasmine fruit, 25 parts of gynostemma pentaphylla, 20 parts of dogwood, 20 parts of hairyvein agrimony, 18 parts of great burdock achene, 12 parts of aloe, 0.8 part of yeast beta-glucan, 0.3 part of honey and 0.02 part of borneol.

Further, the feed comprises the following raw materials in parts by weight: 35 parts of wild buckwheat rhizome, 31 parts of cape jasmine fruit, 30 parts of gynostemma pentaphylla, 25 parts of dogwood, 25 parts of hairyvein agrimony, 20 parts of great burdock achene, 15 parts of aloe, 1.5 parts of yeast beta-glucan, 0.5 part of honey and 0.03 part of borneol.

Furthermore, the traditional Chinese medicine biological preparation with the repairing and inhibiting effects on cervical erosion and HPV can exist in the forms of gel, cream, medicinal powder or vaginal effervescent tablets and the like.

The invention also provides a method for preparing the traditional Chinese medicine biological preparation with the repairing and inhibiting effects on cervical erosion and HPV, which comprises the following steps,

s1, cleaning rhizoma Fagopyri Dibotryis, fructus Gardeniae, herba Gynostemmatis, Corni fructus, herba et Gemma Agrimoniae, fructus Arctii, and Aloe, air drying, pulverizing, and grinding into coarse powder;

s2, obtaining superfine wall-breaking powder with a particle size less than 50nm by using a vibration grinding type superfine pulverizer;

s3, adding an alcohol solvent for reflux extraction, collecting an extracting solution, and extracting under vacuum to obtain a concentrated mixed extract;

s4, dissolving yeast beta-glucan, honey and borneol in purified water, adding the concentrated mixed extract after the yeast beta-glucan, the honey and the borneol are completely dissolved, and stirring for 1-2 h for homogenization;

s5, adding a gel excipient (5% alginate), stirring for 30-60 min, homogenizing, adding triethanolamine to adjust the pH value to an optimal value, standing for 6-12h to obtain a finished product, and hermetically storing.

Further, in the step S1, the ventilation drying is carried out for 12-24h under the precondition of 25-35 ℃; pulverizing at 6000-10000r/min for 60-120s to obtain coarse powder.

Further, in the step S2, the superfine grinding is carried out for 60-120S under the conditions of-10 ℃ to 10 ℃ and 800-1200 r/min.

Further, in the step S3, reflux extraction is respectively carried out for 2 times by 8 times and 5 times of ethanol, and each time lasts for 2-3 hours; the obtained concentrated extract has a density of 1.15g/cm³The error is controlled within +/-0.05 percent.

Further, the yeast beta-glucan, honey and borneol in the step S4 are analytically pure.

Further, the adjuvants in step S5, alginate and triethanolamine, are chemically pure.

Wild buckwheat rhizome: bitter and mild in nature; not only can clear away heat and toxic material, remove dampness and discharge pus, but also can remove stasis and relieve pain; it is mainly used for treating heat toxin dysentery, scrofula, carbuncle, swelling, sore, snake and insect bite, etc.

Gardenia: bitter and cold in nature; clearing away heat, promoting urination, cooling blood, removing toxic substances, relieving swelling and pain; clearing away the fire of heart, lung and triple energizer and eliminating dampness and heat of bladder; can be used for treating jaundice due to damp-heat pathogen, stranguria due to blood pathogen, and sore due to toxic heat.

Gynostemma pentaphylla: sweet and bitter taste and slightly cold nature; clearing away heat and toxic material, invigorating qi and invigorating spleen; has effects in tranquilizing mind, lowering blood pressure, and resisting cancer; it can be used for treating deficiency of both qi and fluid, cough due to dry phlegm, cancer, sore, and carbuncle due to toxic heat.

Dogwood: sour, sweet, slightly warm; astringe and relieve depletion, and tonify liver and kidney; it is a combination of herbs to warm and tonify yin and yang, tonify kidney yang, and tonify kidney essence. It can be used for treating female metrorrhagia and menorrhagia.

Agrimony: bitter, astringent and mild, and can be used for treating cancer; astringe to stop bleeding, check malaria and stop dysentery, kill parasites and remove toxicity; it can be used for treating leukorrhagia, pudendal pruritus, carbuncle, swelling, sore, hematuria, hematochezia, and metrorrhagia.

And (3) burdock fruit: pungent, bitter and cold in flavor; detoxication, eruption, detumescence and sore treatment; it is good at clearing heat-toxicity and relieving constipation; mainly treats wind-heat rash itching, heat-toxin sore swelling, mumps and the like.

Aloe vera: purgative, disinsection, and liver heat clearing; has strong inhibiting effect on gram-positive bacteria, can kill mould and virus, and has repairing effect on damaged skin; it can be used for treating constipation due to heat accumulation, excessive fire of liver channel, tinea, sore, and abdominal pain due to parasitic infestation.

Yeast β -glucan: polysaccharides that are widely found in the cell walls of algae and plants; has effects in enhancing immunity, resisting tumor, bacteria and virus, and promoting wound healing.

Honey: sweet and mild; detoxication, tonifying middle-jiao and relieving urgency, and loosening bowel to relieve constipation; it can be used for treating constipation due to intestinal dryness, abdominal pain, unhealed ulcer, and scald due to hot water and fire.

Borneol: processing Borneolum Syntheticum spice family plants/synthesizing Camphora, clearing heat, relieving pain, inducing resuscitation, and refreshing mind; the oral administration is the essential drug for treating coma and obstruction; it is a good herb for heat-toxicity swelling and pain, and can clear heat and alleviate pain, resolve swelling and promote tissue regeneration for external use.

The invention comprehensively analyzes the characteristics, the taste and the efficacy of the traditional Chinese medicines one by one, continuously improves the traditional Chinese medicines through repeated demonstration and formula improvement, and exerts the advantage of the synergistic formula of the traditional Chinese medicines for improving the curative effect, and the wild buckwheat, the gynostemma pentaphylla and the hairyvein agrimony in the formula have obvious effects of resisting tumors, sterilizing, resisting viruses and regulating the immunity; fructus Gardeniae and fructus Arctii have effects of clearing away toxic materials, relieving swelling and resolving hard mass; the dogwood and the aloe can promote an organism to remove viruses and mutated cells in time through an immune system, protect cervical squamous epithelial tissue, activate peripheral nerves of vaginal tissue, accelerate blood circulation, comprehensively repair vaginal mucosa and regenerate the vaginal mucosa; the yeast beta-glucan, the honey and the borneol can remove blood stasis and promote tissue regeneration, resist bacteria and inflammation, inhibit tumors and reduce the harm of viruses to human bodies; meanwhile, the scheme adopts a nanoscale ultramicro extraction technology to keep the content of the medicinal powder, a reflux extraction technology reduces impurity exudation, a nanometer transdermal absorption technology is used for forming a protective film on the epidermis of an affected part, the adhesion time is long, not only is the diseased part protected, but also the transdermal absorption of the medicament is increased, especially, the deep vaginal administration has obvious effects of repairing cervical erosion and inhibiting HPV, the curative effect is exact, and the recurrence rate can be reduced; the preparation method is simple to operate, convenient to use and worthy of popularization and large-scale production.

Compared with the prior art, the invention has the following beneficial effects:

the active ingredients in the biological preparation can permeate into the bottom layer cells of cervical mucosa through nano transdermal absorption, fully contact and more comprehensively and thoroughly repair the erosion tissues, eliminate viruses, and have the effective rate of more than 96 percent for treating chronic inflammations such as gynecological cervical erosion, vaginitis and the like; has obvious therapeutic effect on HPV infection, has an effective negative conversion rate of more than 90 percent, effectively inhibits the precancerous lesion of cervical carcinoma, and has very important social value and significance.

The preparation method of the traditional Chinese medicine biological preparation provided by the invention is simple to operate, the cost is low, the adopted raw materials are all effective extracted components in common traditional Chinese medicinal materials, the effective extracted components directly act on focus, the first pass effect of liver is avoided, the traditional Chinese medicine biological preparation has better repairing and inhibiting effects on cervical erosion and HPV infection through experiments, has good inhibiting effect on gynecological inflammation caused by other various reasons, has definite curative effect, is convenient to administer, safe and non-irritant, has good stability and high patient compliance, and is suitable for large-scale production.

Drawings

FIG. 1 is a schematic diagram of the formulation process of the biological agent for repairing and inhibiting cervical erosion and HPV in the present invention;

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

Reagents, auxiliary materials and the like used in the present examples were purchased from normal commercial sources unless otherwise specified. The experimental method is not shown in the embodiment, and is generally recommended by the manufacturer or performed according to conventional conditions.

The terms "first" and "second," and the like, in the description and in the claims of embodiments of the present invention are used for distinguishing between different objects and not for describing a particular order of the objects. For example, the first parameter set and the second parameter set, etc. are used to distinguish different parameter sets, rather than to describe a particular order of parameter sets.

In the description of the embodiments of the present invention, the meaning of "a plurality" means two or more unless otherwise specified. For example, a plurality of elements refers to two elements or more. The term "and/or" herein is an association relationship describing an associated object, and means that there may be three relationships, for example, a display panel and/or a backlight, which may mean: there are three cases of a display panel alone, a display panel and a backlight together, and a backlight alone. The symbol "/" herein denotes a relationship in which the associated object is or, for example, input/output denotes input or output.

In the present embodiments, words such as "exemplary" or "for example" are used to indicate examples, illustrations or descriptions. Any embodiment or design described as "exemplary" or "e.g.," an embodiment of the present invention is not necessarily to be construed as preferred or advantageous over other embodiments or designs. Rather, use of the word "exemplary" or "such as" is intended to present relevant concepts in a concrete fashion.

The following examples are not intended to limit the scope of the present invention, nor are the steps described to limit the order of execution. Modifications of the invention which are obvious to those skilled in the art in view of the prior art are also within the scope of the invention as claimed.

In the existing medical technology, patients suffering from cervical erosion and HPV infection are treated by operation, and although the treatment effect of the operation method for treating the cervical erosion of women is quick, doctors do not recommend the treatment method for women who are not married and have a fertility requirement. Because the harm of the cervical erosion operation is mainly caused by the treatment in the way, the deformation or the adhesion of the cervical orifice of the female can be caused, which can influence the future fertility of the female invisibly; in addition, after the operation treatment, the traditional Chinese medicine composition can bring large trauma to a patient, reduce the physical constitution of the patient, reduce the immunity and cannot be recovered in a short time; therefore, the treatment of cervical erosion and HPV infection for women by adopting an operation mode needs to be careful.

The invention provides a traditional Chinese medicine biological preparation, the preparation method is simple to operate, the cost is low, the adopted raw materials are all effective extracted components in common traditional Chinese medicinal materials, the effective extracted components directly act on the focus, the liver first-pass effect is avoided, the traditional Chinese medicine biological preparation has better repairing and inhibiting effects on cervical erosion and HPV infection through experiments, has good inhibiting effects on gynecological inflammation caused by other various reasons, and is accurate in curative effect, convenient to administer, safe, free of stimulation, good in stability, high in patient compliance and suitable for large-scale batch production.

Example 1

As shown in fig. 1, the biological agent for repairing and inhibiting cervical erosion and HPV described in this embodiment includes the following raw materials in parts by weight: 28-35 parts of wild buckwheat rhizome, 25-31 parts of cape jasmine fruit, 22-30 parts of gynostemma pentaphylla, 18-25 parts of dogwood, 15-25 parts of hairyvein agrimony, 15-20 parts of burdock, 10-15 parts of aloe, 0.5-1.5 parts of yeast beta-glucan, 0.2-0.5 part of honey and 0.015-0.03 part of borneol.

Example 2

As shown in fig. 1, the biological agent for repairing and inhibiting cervical erosion and HPV described in this embodiment includes the following raw materials by weight: 30kg of wild buckwheat rhizome, 28kg of cape jasmine fruit, 25kg of gynostemma pentaphylla, 20kg of dogwood, 20kg of hairyvein agrimonia herb and bud, 18kg of burdock, 12kg of aloe, 0.8kg of yeast beta-glucan, 0.3kg of honey and 0.02kg of borneol.

Example 3

As shown in fig. 1, the method for formulating the biological agent capable of repairing and inhibiting cervical erosion and HPV described in this embodiment is based on the biological agent in embodiment 2, and includes the following steps:

s1, cleaning rhizoma Fagopyri Dibotryis 30kg, fructus Gardeniae 28kg, herba Gynostemmatis 25kg, Corni fructus 20kg, herba et Gemma Agrimoniae 20kg, fructus Arctii 18kg, and Aloe 12kg, air drying, pulverizing, and grinding into coarse powder;

s2, obtaining ultramicro wall-broken powder with a particle size less than 50nm by using a vibration grinding type ultramicro pulverizer;

s3, adding alcohol solvent, reflux extracting, collecting extractive solution, and vacuum pumping to obtain concentrated mixed extract 1.15 + -0.05 g/cm³；

S4, dissolving yeast beta-glucan 0.8kg, honey 0.3kg and borneol 0.02kg in purified water, adding the concentrated mixed extract after all the yeast beta-glucan, honey and borneol are dissolved, and stirring for 1-2 h for homogenization;

s5, adding a gel excipient (5% alginate), stirring for 30-60 min, homogenizing, adding triethanolamine to adjust the pH value to an optimal value, standing for 6-12h to obtain a finished product, and sealing for storage.

The preparation prepared by the preparation method is semitransparent light yellow brown gel with faint scent.

Specifically, in the step S1, the ventilation drying is drying for 12-24h under the precondition of 25-35 ℃; at 6000-100Pulverizing at 00r/min for 60-120s to obtain coarse powder; in the step S2, the superfine grinding is carried out for 60-120S under the conditions of-10 ℃ and 800-1200 r/min; in the step S3, reflux extraction is performed for 2 times by respectively adopting 8 times and 5 times of ethanol, and each time lasts for 2-3 h; the obtained concentrated extract has a density of 1.15g/cm³Controlling the error within plus or minus 0.05 percent; the yeast beta-glucan, the honey and the borneol in the step S4 are analytically pure; the adjuvants in step S5, alginate and triethanolamine, are chemically pure.

Example 4

As shown in fig. 1, the biological preparation of traditional Chinese medicine for repairing and inhibiting cervical erosion and HPV in this embodiment includes the following raw materials by weight: 28kg of wild buckwheat rhizome, 25kg of cape jasmine fruit, 22kg of gynostemma pentaphylla, 18kg of dogwood fruit, 15kg of hairyvein agrimony, 15kg of great burdock achene, 10kg of aloe, 0.5kg of yeast beta-glucan, 0.2kg of honey and 0.015kg of borneol.

Example 5

As shown in fig. 1, the method for formulating the biological agent capable of repairing and inhibiting cervical erosion and HPV described in this embodiment is based on the biological agent in embodiment 4, and includes the following steps:

s1, cleaning rhizoma Fagopyri Dibotryis 28kg, fructus Gardeniae 25kg, herba Gynostemmatis 22kg, Corni fructus 18kg, herba et Gemma Agrimoniae 15kg, fructus Arctii 15kg, and Aloe 10kg, air drying, pulverizing, and grinding into coarse powder;

s3, adding alcohol solvent for reflux extraction, collecting extractive solution, and vacuum-pumping to obtain concentrated mixed extract 1.15 + -0.05 g/cm³；

S4, dissolving yeast beta-glucan 0.5kg, honey 0.2kg and borneol 0.015kg in purified water, adding the concentrated mixed extract after all the yeast beta-glucan, honey and borneol are dissolved completely, and stirring for 1-2 h for homogenization;

Specifically, in the step S1, the ventilation drying is carried out for 12-24h under the premise of 25-35 ℃; crushing for 60-120s under the condition of 6000 plus 10000r/min to obtain coarse powder; in the step S2, the superfine grinding is carried out for 60-120S under the conditions of-10 ℃ and 800-1200 r/min; in the step S3, reflux extraction is performed for 2 times by respectively adopting 8 times and 5 times of ethanol, and each time lasts for 2-3 hours; the obtained concentrated extract has a density of 1.15g/cm³Controlling the error within plus or minus 0.05 percent; the yeast beta-glucan, the honey and the borneol in the step S4 are analytically pure; the auxiliary materials of alginate and triethanolamine in the step S5 are chemically pure.

Example 6

As shown in fig. 1, the traditional Chinese medicine biological preparation for repairing and inhibiting cervical erosion and HPV in this embodiment includes the following raw materials by weight: 35kg of wild buckwheat rhizome, 31kg of cape jasmine fruit, 30kg of gynostemma pentaphylla, 25kg of dogwood fruit, 25kg of hairyvein agrimony, 20kg of burdock fruit, 15kg of aloe, 1.5kg of yeast beta-glucan, 0.5kg of honey and 0.03kg of borneol.

Example 7

As shown in fig. 1, the method for formulating the biological agent for repairable inhibition of cervical erosion and HPV, which is described in this embodiment, is based on the biological agent in embodiment 6, and includes the following steps:

s1, cleaning wild buckwheat rhizome 35kg, cape jasmine 31kg, gynostemma pentaphylla 30kg, dogwood 25kg, agrimonia pilosa ledeb 25kg, burdock fruit 20kg and aloe 15kg, drying in the air, pulverizing and grinding into coarse powder;

s2, obtaining superfine wall-broken powder with a particle size less than 50nm by using a vibration grinding type superfine pulverizer;

S4, dissolving yeast beta-glucan 1.5kg, honey 0.5kg and borneol 0.03kg in purified water, adding the concentrated mixed extract after all the yeast beta-glucan, honey 0.5kg and borneol are dissolved, and stirring for 1-2 h for homogenization;

The preparation prepared by the preparation method is semitransparent light yellowish brown gel with faint scent.

Specifically, in the step S1, the ventilation drying is carried out for 12-24h under the premise of 25-35 ℃; crushing for 60-120s under the condition of 6000 plus 10000r/min to obtain coarse powder; in the step S2, the superfine grinding is carried out for 60-120S under the conditions of-10 ℃ and 800-1200 r/min; in the step S3, reflux extraction is performed for 2 times by respectively adopting 8 times and 5 times of ethanol, and each time lasts for 2-3 h; the obtained concentrated extract has a density of 1.15g/cm³The error is controlled within +/-0.05 percent; the yeast beta-glucan, the honey and the borneol in the step S4 are analytically pure; the auxiliary materials of alginate and triethanolamine in the step S5 are chemically pure.

The specific use method of the traditional Chinese medicine biological preparation having the repairing and inhibiting effects on cervical erosion and HPV comprises the following steps: after vulva is cleaned, the gel is slowly and lightly pushed into the affected part of vagina.

By combining the technical schemes in the embodiments 1 to 7, the preparation method of the pharmaceutical composition is simple to operate and low in cost, the adopted raw materials are all effective extracted components in common Chinese medicinal materials, the raw materials directly act on the focus, the liver first-pass effect is avoided, the pharmaceutical composition has a good repairing and inhibiting effect on cervical erosion and HPV infection through tests, has a good inhibiting effect on gynecological inflammation caused by other various reasons, is exact in curative effect, convenient to administer, safe, free of stimulation, good in stability, high in patient compliance, and suitable for batch large-scale production.

The invention comprehensively analyzes the nature, the taste and the efficacy of the traditional Chinese medicine one by one, is continuously perfect through repeated demonstration and formula improvement, and exerts the advantage of the efficacy enhancement of the traditional Chinese medicine synergistic formula, and the wild buckwheat rhizome, the fiveleaf gynostemma herb and the hairyvein agrimony in the formula have obvious efficacies of resisting tumors, sterilizing, resisting viruses and regulating immunity; fructus Gardeniae and fructus Arctii have effects of clearing away toxic materials, relieving swelling and resolving hard mass; the dogwood and the aloe can promote an organism to remove viruses and mutated cells in time through an immune system, protect cervical squamous epithelial tissue, activate peripheral nerves of vaginal tissue, accelerate blood circulation, comprehensively repair vaginal mucosa and regenerate the vaginal mucosa; the yeast beta-glucan, the honey and the borneol can remove blood stasis and promote tissue regeneration, resist bacteria and inflammation, inhibit tumors and reduce the harm of viruses to human bodies; meanwhile, the scheme adopts a nanoscale ultramicro extraction technology to reserve the content of the medicinal powder, reduces impurity exudation by a reflux extraction technology, forms a protective film on the epidermis of an affected part by using a nanometer transdermal absorption technology, has long adhesion time, not only protects a diseased part, but also increases the transdermal absorption of the medicament, particularly has obvious effects of repairing cervical erosion and inhibiting HPV (human papilloma virus) by deep delivery of the medicament in vagina, has definite curative effect, and can reduce the recurrence rate; the preparation method is simple to operate, convenient to use and worthy of popularization and large-scale production.

The preparation method of the pharmaceutical composition is simple to operate and low in cost, the adopted raw materials are all effective extracted components in common Chinese medicinal materials, and the pharmaceutical composition directly acts on a focus to avoid the first pass effect of the liver, has a good repairing and inhibiting effect on cervical erosion and HPV infection through experiments, has a good inhibiting effect on gynecological inflammation caused by other various reasons, is exact in curative effect, convenient to administer, safe, free of stimulation, good in stability, high in patient compliance and suitable for batch large-scale production.

While embodiments of the present invention have been described, the present invention is not limited to the above embodiments, which are illustrative, rather than restrictive, and many modifications may be made by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. The biological preparation capable of repairing and inhibiting cervical erosion and HPV is characterized by comprising the following raw materials in parts by weight: 28-35 parts of wild buckwheat rhizome, 25-31 parts of cape jasmine fruit, 22-30 parts of gynostemma pentaphylla, 18-25 parts of dogwood, 15-25 parts of hairyvein agrimony, 15-20 parts of burdock, 10-15 parts of aloe, 0.5-1.5 parts of yeast beta-glucan, 0.2-0.5 part of honey and 0.015-0.03 part of borneol.

2. The biological preparation for repairable inhibition of cervical erosion and HPV according to claim 1, characterized by comprising the following raw materials in parts by weight: 28 parts of wild buckwheat rhizome, 25 parts of cape jasmine fruit, 22 parts of gynostemma pentaphylla, 18 parts of dogwood, 15 parts of hairyvein agrimony, 15 parts of great burdock achene, 10 parts of aloe, 0.5 part of yeast beta-glucan, 0.2 part of honey and 0.015 part of borneol.

3. The biological preparation for repairable inhibition of cervical erosion and HPV according to claim 1, characterized by comprising the following raw materials in parts by weight: 30 parts of wild buckwheat rhizome, 28 parts of cape jasmine fruit, 25 parts of gynostemma pentaphylla, 20 parts of dogwood, 20 parts of hairyvein agrimony, 18 parts of burdock, 12 parts of aloe, 0.8 part of yeast beta-glucan, 0.3 part of honey and 0.02 part of borneol.

4. The biological preparation for repairable inhibition of cervical erosion and HPV according to claim 1, characterized by comprising the following raw materials in parts by weight: 35 parts of wild buckwheat rhizome, 31 parts of cape jasmine fruit, 30 parts of gynostemma pentaphylla, 25 parts of dogwood, 25 parts of hairyvein agrimony, 20 parts of burdock, 15 parts of aloe, 1.5 parts of yeast beta-glucan, 0.5 part of honey and 0.03 part of borneol.

5. The method for formulating a biological agent for repairable inhibition of cervical erosion and HPV according to any one of claims 1 to 4, characterized in that it comprises the following steps:

s1, washing rhizoma Fagopyri Dibotryis, fructus Gardeniae, herba Gynostemmatis, Corni fructus, herba et Gemma Agrimoniae, fructus Arctii, and Aloe, air drying, pulverizing, and grinding into coarse powder;

s3, adding alcohol solvent for reflux extraction, collecting the extract, and extracting under vacuum to obtain concentrated mixed extract;

6. The method for preparing the biological preparation capable of repairing and inhibiting cervical erosion and HPV as claimed in claim 5, wherein in the step S1, the ventilation drying is carried out for 12-24h at 25-35 ℃; pulverizing at 6000-10000r/min for 60-120s to obtain coarse powder.

7. The preparation method of the biological agent for repairing and inhibiting cervical erosion and HPV according to claim 5, wherein in the step S2, the superfine grinding is carried out for 60-120S at-10 ℃ to 10 ℃ and at 800-1200 r/min.

8. The method for preparing a biological agent for repairing and inhibiting cervical erosion and HPV according to claim 5, wherein in the step S3, the reflux extraction is performed for 2 times with 8 times and 5 times of ethanol for 2-3 hours each time; the obtained concentrated extract has a density of 1.15g/cm³The error is controlled within +/-0.05 percent.

9. The method for preparing a biological agent for repairable inhibition of cervical erosion and HPV according to claim 5, wherein the yeast β -glucan, honey and borneol in the step S4 are analytically pure.

10. The method for preparing a biological preparation capable of repairing and inhibiting cervical erosion and HPV as claimed in claim 5, wherein the auxiliary materials alginate and triethanolamine in step S5 are chemically pure.