CN114766361B - Lily bamboo tissue culture seedling cultivation device and application method thereof - Google Patents

Lily bamboo tissue culture seedling cultivation device and application method thereof Download PDF

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Publication number
CN114766361B
CN114766361B CN202210430567.7A CN202210430567A CN114766361B CN 114766361 B CN114766361 B CN 114766361B CN 202210430567 A CN202210430567 A CN 202210430567A CN 114766361 B CN114766361 B CN 114766361B
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arc
shaped water
lily
water bag
shaped
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CN114766361A (en
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周日花
李贵雨
林妃
高宏茂
唐跃东
谭乐和
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Hainan Rezuo Liangyuan Seed Industry Technology Co ltd
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Hainan Rezuo Liangyuan Seed Industry Technology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/003Cutting apparatus specially adapted for tissue culture
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a lily bamboo tissue culture seedling cultivation device which comprises a plurality of groups of enclosing mechanisms for enclosing lily bamboo branches, wherein different enclosing mechanisms are connected together through a connecting mechanism, a moving mechanism is arranged on the connecting mechanism positioned at the lowest part, each enclosing mechanism comprises a first arc-shaped water bag, a second arc-shaped water bag, a third arc-shaped water bag and a fourth arc-shaped water bag, the first arc-shaped water bags, the second arc-shaped water bags and the third arc-shaped water bags are fixedly connected together, the fourth arc-shaped water bags are respectively connected with the second arc-shaped water bags and the third arc-shaped water bags, and the first arc-shaped water bags, the second arc-shaped water bags, the third arc-shaped water bags and the fourth arc-shaped water bags are enclosed to form a ring shape.

Description

Lily bamboo tissue culture seedling cultivation device and application method thereof
Technical Field
The invention relates to the field of agricultural cultivation, in particular to a lily bamboo tissue culture seedling cultivation device.
Background
Lily bamboo is a perennial evergreen shrub or small arbor of Agave, dragon and genus, branches are arranged on the vertical upper part, internodes are short, and a large amount of lily bamboo is introduced and cultivated in the south China. Lily bamboo is high in temperature and humidity, suitable for growing at 20-28 ℃, drought-resistant and moisture-resistant, suitable for penumbra, and not suitable for strong direct sunlight, and has overwintering requirements above 12 ℃ and not strict requirements on soil and fertilizer. The national lily bamboo market is huge in scale and high in market seedling demand, but the existing cutting means cannot meet the market demand, so that the quality and yield of seedlings are improved by means of an in-vitro rapid propagation technology, the virus carrying rate can be reduced, the activity of the seedlings is improved, and the quality of the seedlings is ensured by applying a tissue culture technology; secondly, can enlarge the reproduction base number, greatly reduced manufacturing cost, and when carrying out tissue extraction work to lily bamboo, often adopt artifical cutting to carry out the extraction of tissue, still need transport to aseptic space after handling the plant tissue that draws out and carry out subsequent isolated culture, and inefficiency, moreover, be unfavorable to carry out excessive extraction with any type plant tissue on same plant, easily lead to lily bamboo growth rate to reduce and mortality promotion, current extraction equipment is when being used for cutting the branch, because lily bamboo branch is thinner, when cutting, because the blade can insert in the branch of lily bamboo gradually, lead to the blade can extrude the lily bamboo main part that has not cut gradually, appear because of the secondary injury that extrudes and bring when cutting, the tissue after cutting is difficult for peeling off with the bulk phase simultaneously, moreover, in order to guarantee that the branch of lily bamboo can keep healthy after extracting some tissues, need reduce the volume of single point sample as far as possible, simultaneously guarantee that the length of sample, width and height are in reasonable scope.
Disclosure of Invention
Aiming at the prior problems, the technical problem to be solved by the invention is that the lily bamboo tissue culture seedling cultivation device can reduce the extrusion of the tissues remained on the branches during cutting, reduce secondary injury, simultaneously enable the cut tissues to be easily separated, and is suitable for cutting the tissues of the branches with thinner branches.
The invention provides a lily bamboo tissue culture seedling cultivation device, which comprises a plurality of groups of enclosing mechanisms for enclosing lily bamboo branches, wherein different enclosing mechanisms are connected together through a connecting mechanism, a moving mechanism is arranged on the connecting mechanism positioned at the lowest part, each enclosing mechanism comprises a first arc-shaped water bag, a second arc-shaped water bag, a third arc-shaped water bag and a fourth arc-shaped water bag, the first arc-shaped water bag, the second arc-shaped water bag and the third arc-shaped water bag are fixedly connected together, the fourth arc-shaped water bag is respectively connected with the second arc-shaped water bag and the third arc-shaped water bag, the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag are enclosed to be in a ring shape, the novel water tank is characterized in that a plurality of independent cavities are formed in the first arc water tank, the second arc water tank, the third arc water tank and the fourth arc water tank, a rectangular frame is arranged on the fourth arc water tank, a plurality of hollow grooves are formed in the rectangular frame, through grooves are formed in the end portions of the rectangular frame, the through grooves are communicated with each other, elastic blades which are abutted to the through grooves and can move relatively are arranged in the through grooves, a bag body is arranged in the hollow grooves, the bag body is connected with an untrimmed part of the elastic blades, the first arc water tank, the second arc water tank, the third arc water tank, the fourth arc water tank and the bag body are all communicated with an external water source, and the moving mechanism is electrically connected with an external power supply.
Further, coupling mechanism includes a plurality of first coupling assembling and a plurality of second coupling assembling, first coupling assembling includes head rod and a plurality of first arc connecting plate, the tip of head rod with first arc connecting plate links to each other, first arc connecting plate respectively with first arc water pocket second arc water pocket with the third arc water pocket links to each other, second coupling assembling includes second connecting rod and a plurality of second arc connecting plate, the tip of second connecting rod with the second arc connecting plate links to each other, the second arc connecting plate respectively with the fourth arc water pocket links to each other.
Further, the moving mechanism comprises a plurality of linear guide rails, a first linear motor is arranged on the linear guide rails, a rotating mechanism is arranged at the top of the first linear motor, one rotating mechanism is connected with the first arc-shaped connecting plate located at the lowest position, the other rotating mechanism is connected with the second arc-shaped connecting plate located at the lowest position, the first linear motor and the rotating mechanism are electrically connected with an external power supply, and the first linear motor is connected with external signals.
Further, the rotating mechanism comprises an arc-shaped guide rail and a second linear motor, wherein the arc-shaped guide rail is fixedly connected with the top of the first linear motor, the second linear motor is arranged on the arc-shaped guide rail, one of the second linear motor is connected with the first arc-shaped connecting plate positioned at the lowest position, the other second linear motor is connected with the second arc-shaped connecting plate positioned at the lowest position, the second linear motor is electrically connected with an external power supply, and the second linear motor is connected with external signals.
Further, be equipped with a plurality of first electric telescopic handle between the linear guide, first electric telescopic handle's flexible end and one of them side linear guide links to each other, first electric telescopic handle's stiff end with the opposite side linear guide links to each other, first electric telescopic handle is connected with external power supply electricity, first electric telescopic handle is connected with external signal.
Further, the bottom of linear guide is equipped with the carrier that is used for bearing lily bamboo cultivated in a pot, linear guide's bottom is equipped with the slider, be equipped with the spout on the carrier, the slider is in slide in the spout, the bottom of carrier is equipped with a plurality of bracing pieces, the bottom of bracing piece is equipped with the universal wheel.
Further, be equipped with the fixed plate that links to each other with it on the arc guide rail, be equipped with the backup pad on the fixed plate, be equipped with the second electric telescopic handle that links to each other with it in the backup pad, the flexible end of second electric telescopic handle is equipped with one side open-ended casing, opening one side of casing with the rectangle frame is aligned, be equipped with the negative pressure pump that is linked together with it on the casing, second electric telescopic handle is connected with external power supply electricity, second electric telescopic handle is connected with external signal.
A use method of a lily bamboo tissue culture seedling cultivation device comprises the following steps of S1: the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag enclose the branches of the lily bulbs; s2: the position of the fourth arc-shaped water bag is opposite to the direction of the growth inclination direction of the lily bulbs per se; s3: filling the cavities in the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag with equal quantity; s4: further filling a water source into the cavity in the fourth arc-shaped water bag, so that the fourth arc-shaped water bag is continuously expanded and extrudes the branches of the lily bulbs, and the branches of the lily bulbs are in a bending state;
s5: the elastic blade moves towards the branch direction of the lily bamboo after being extruded by the water filled bag body until the elastic blade cuts open and goes deep into the lily bamboo branch; s6: and cutting the branches of the lily bamboos through different elastic blades.
The invention has the beneficial effects that:
the lily bamboo tissue culture seedling cultivating device of the invention can move to the cultivating position of lily bamboo by the driving action of the moving mechanism, the position of the fourth arc-shaped water bag is determined according to the growth inclined direction of the lily bamboo, the position of the fourth arc-shaped water bag is opposite to the growth inclined direction of the lily bamboo, then the branches of the lily bamboo are enclosed, the lily bamboo can be effectively wrapped by the cooperation of the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag, then the cavities in the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag are continuously filled with water by external water sources, so that the branches of the lily bamboo are tightly adhered and wrapped by the water filling expansion of the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag, ensuring that the branches of lily bamboos can be sufficiently straightened and fixed at the moment, stopping water source inflow of the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag, further filling the water source into the cavity in the fourth arc-shaped water bag through an external water source, enabling the fourth arc-shaped water bag to continuously expand and squeeze the branches of lily bamboos, enabling the partial branches of lily bamboos to be in a bending state, enabling the surfaces of the lily branches to be in a tightening state, then filling water into the bag body in the hollow groove in the horizontal direction, further enabling the elastic blade to move towards the branches of lily bamboos until being cut and penetrated into the lily branches after being squeezed by the bag body, because the surface of the lily bamboo branch at the cut side is in a stretched and tensed state, other tissues on the branch can be separated from the elastic blade under the action of external force, the cut branch tissues are not extruded when the elastic blade is gradually deepened, secondary damage is reduced, and then water in the water filled bag body can be extracted, so that the elastic blade is reset; when a cut in the vertical direction is required to be cut, the second arc-shaped water bag on one side is filled with water for the second time, so that the lily bamboo shoots incline towards the other direction under the combined action of the second arc-shaped water bag and the fourth arc-shaped water bag, at the moment, the bag bodies in the hollow groove on the same side in the vertical direction as the second arc-shaped water bag can be filled with water, the elastic blades are further enabled to move towards the lily bamboo shoots until cut and penetrate into the lily bamboo shoots after being extruded by the bag bodies, the surfaces of the cut lily bamboo shoots are in a stretched tight state under the combined action of the second arc-shaped water bag and the fourth arc-shaped water bag, other tissues on the shoots can be separated from the elastic blades under the action of external force, the elastic blades are not extruded when the elastic blades are gradually deepened, and secondary injuries caused by the second cutting work are reduced; then, the water of the second arc-shaped water bag is discharged to the amount before secondary water filling and is filled towards the third arc-shaped water bag, so that the lily bamboo branch is inclined towards the third direction under the combined action of the third arc-shaped water bag and the fourth arc-shaped water bag, at the moment, the bag body in the hollow groove which is positioned in the vertical direction and at the same side of the third arc-shaped water bag is filled with water, the elastic blade is further moved towards the lily bamboo branch direction until the lily bamboo branch is cut and goes deep into the lily bamboo branch after being extruded by the bag body, the cut lily bamboo branch surface is in a stretched and tensed state under the combined action of the third arc-shaped water bag and the fourth arc-shaped water bag, so that other tissues on the lily branch can be separated from the elastic blade under the action of external force, the branches are not extruded when the elastic blades are gradually deepened, the secondary damage caused by the third cutting operation is reduced, and because the lily bamboo branches can be in a tense state during cutting, the cut tissues in the cut area can be separated from the branches under the action of the force generated by stretching and tense, the cutting operation is safer and more reliable, the condition that the lily bamboo is easy to die due to large manual operation error is reduced, the surfaces of the branches on the side opposite to the inclined direction are cut during cutting, the lily bamboo is not pressed more by gravity, the problem that the cut position is extruded to generate secondary damage due to the action of gravity is avoided when the lily bamboo is cut on the same side of the inclined direction, the device can be applied to the tissue extraction of the lily bamboo, thereby facilitating the subsequent tissue culture work.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only preferred embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a whole structure diagram of a lily bamboo tissue culture seedling cultivation device of the invention;
FIG. 2 is a cross-sectional view of a rectangular frame of a lily bamboo tissue culture seedling cultivation device of the invention;
FIG. 3 is a cross-sectional view of a first or second or third or fourth arcuate water bladder of a lily bamboo tissue culture seedling culturing device of the present invention.
In the figure, 1 is a first arc water bag, 2 is a second arc water bag, 3 is a third arc water bag, 4 is a fourth arc water bag, 5 is a cavity, 6 is a rectangular frame, 7 is a hollow groove, 8 is a through groove, 9 is an elastic blade, 10 is a bag body, 11 is a first connecting rod, 12 is a first arc connecting plate, 13 is a second connecting rod, 14 is a second arc connecting plate, 15 is a linear guide rail, 16 is a first linear motor, 17 is an arc guide rail, 18 is a second linear motor, 19 is a first electric telescopic rod, 20 is a bearing frame, 21 is a sliding block, 22 is a sliding groove, 23 is a supporting rod, 24 is a universal wheel, 25 is a fixed plate, 26 is a shell, 27 is a negative pressure pump, 28 is a supporting plate, and 29 is a second electric telescopic rod.
Detailed Description
For a better understanding of the technical content of the present invention, specific examples are provided below and the present invention is further described with reference to the accompanying drawings.
Referring to fig. 1 to 3, the invention provides a lily bamboo tissue culture seedling cultivation device, which comprises a plurality of groups of enclosing mechanisms for enclosing lily bamboo branches, wherein different enclosing mechanisms are connected together through a connecting mechanism, a moving mechanism is arranged on the connecting mechanism positioned at the lowest part, the enclosing mechanism comprises a first arc-shaped water bag 1, a second arc-shaped water bag 2, a third arc-shaped water bag 3 and a fourth arc-shaped water bag 4, the first arc-shaped water bag 1, the second arc-shaped water bag 2 and the third arc-shaped water bag 3 are fixedly connected together, the fourth arc-shaped water bag 4 is respectively connected with the second arc-shaped water bag 2 and the third arc-shaped water bag 3, the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4 enclose a ring shape, the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4 are internally provided with a plurality of independent cavities 5, the fourth arc-shaped water bag 4 is provided with a rectangular frame 6, a plurality of hollow grooves 7 are arranged in the rectangular frame 6, the end parts of the rectangular frame 6 are provided with through grooves 8, the through grooves 8 are communicated with each other, elastic blades 9 which are abutted to the through grooves 8 and can relatively move are arranged in the through grooves 8, a bag body 9 is arranged in the hollow grooves 7, the bag body 9 is connected with the non-cutting edge parts of the elastic blades 9, the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3, the fourth arc-shaped water bag 4 and the bag body 9 are all communicated with an external water source, the moving mechanism is electrically connected with an external power supply, the device can move to the culturing position of lily through the driving function of the moving mechanism, determining the position of the fourth arc-shaped water bag 4 according to the growth inclination direction of the lily bamboo, enabling the position of the fourth arc-shaped water bag 4 to be opposite to the growth inclination direction of the lily bamboo, then enclosing the branches of the lily bamboo, enabling the lily bamboo to be effectively wrapped through the cooperation of the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4, continuously filling water into the cavities 5 in the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4 through an external water source, enabling the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4 to be inflated and enabling the branches of the lily bamboo to be tightly attached and wrapped, ensuring that the branches of the lily bamboo can be fully straightened and fixed at the moment, then the water sources of the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4 are stopped from flowing in, then the water sources are further filled into the cavity 5 in the fourth arc-shaped water bag 4 through an external water source, so that the fourth arc-shaped water bag 4 continuously expands and presses the branches of the lily bulbs, the partial branches of the lily bulbs are in a bending state, the surfaces of the lily bulbs are in a tightening state, then the air bags 9 in the hollow grooves 7 in the horizontal direction are filled with water, the elastic blades 9 are further moved in the directions of the branches of the lily bulbs after being pressed by the air bags 9 until the cut and deep into the lily bulbs, and because the surfaces of the lily bulbs on the cut side are in a tightening state under tension at this time, other tissues on the branches can be separated from the elastic blade 9 under the action of external force, the cut branch tissues are not extruded when the elastic blade 9 is gradually deepened, secondary damage is reduced, and then water in the water filled bag body 9 can be extracted, so that the elastic blade 9 is reset; when the incision in the vertical direction is required to be cut, the second arc-shaped water bag 2 on one side is filled with water for the second time, so that the lily bamboo shoots are inclined towards the other direction under the combined action of the second arc-shaped water bag 2 and the fourth arc-shaped water bag 4, at the moment, the bag body 9 in the hollow groove 7 which is positioned in the vertical direction and is positioned on the same side as the second arc-shaped water bag 2 is filled with water, the elastic blade 9 is further moved towards the lily bamboo shoots after being extruded by the bag body 9 until the lily bamboo shoots are cut and extend into the lily bamboo shoots, the surfaces of the cut lily bamboo shoots are in a stretched state under the combined action of the second arc-shaped water bag 2 and the fourth arc-shaped water bag 4, other tissues on the lily shoots can be separated from the elastic blade 9 under the action of external force, the lily shoots are not extruded by the elastic blade 9 in a gradually deep way, and secondary injuries caused by the secondary cutting work are reduced; then, by discharging the water of the second arc-shaped water bag 2 to the amount before secondary water filling and filling the water towards the third arc-shaped water bag 3, the lily bamboo shoots are inclined towards the third direction under the combined action of the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4, at the moment, the bag body 9 in the hollow groove 7 which is positioned in the vertical direction and at the same side of the third arc-shaped water bag 3 can be filled with water, the elastic blade 9 moves towards the lily bamboo shoots after being extruded by the bag body 9 until the cut lily bamboo shoots are cut and penetrated into the lily bamboo shoots, the cut lily bamboo shoots at the moment are in a stretched state under the combined action of the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4, so that other tissues on the lily shoots can be separated from the elastic blade 9 under the action of external force, the branches are not extruded when the elastic blade 9 is gradually deepened, the secondary damage caused by the third cutting operation is reduced, and because the lily bamboo branches can be in a tense state when being cut, the cut tissues in the cut area can be separated from the branches under the action of the force generated by stretching and tense, the cutting operation is safer and more reliable, the condition that the lily bamboo is easy to die due to large manual operation error is reduced, the surfaces of the branches on the side opposite to the inclined direction are cut when the lily bamboo is cut, the lily bamboo is not pressed more bent under the action of gravity, the problem that the cut position is extruded and secondary damage is caused under the action of gravity is avoided when the lily bamboo is cut on the same side of the inclined direction is avoided, the device can be additionally applied to the tissue extraction of the lily bamboo, thereby facilitating the subsequent tissue culture work.
Specifically, coupling mechanism includes a plurality of first coupling assembling and a plurality of second coupling assembling, first coupling assembling includes head rod 11 and a plurality of first arc connecting plate 12, head rod 11's tip with first arc connecting plate 12 links to each other, first arc connecting plate 12 respectively with first arc water pocket 1 second arc water pocket 2 with third arc water pocket 3 links to each other, second coupling assembling includes second connecting rod 13 and a plurality of second arc connecting plate 14, the tip of second connecting rod 13 with second arc connecting plate 14 links to each other, second arc connecting plate 14 respectively with fourth arc water pocket 4 links to each other, through head rod 11 with cooperation between the first arc connecting plate 12 can be right first arc water pocket 1 second arc water pocket 2 with third arc water pocket 3 carries out effective support, avoids back inflation down simultaneously, passes through second connecting rod 13 with cooperation of second connecting plate 14 can carry out the expansion to the fourth arc and can carry out the inflation simultaneously more than the high-efficient water-filling point to the high-efficient water sampling to the fourth arc connecting plate 14, thereby can carry out the high-efficient water sampling to the multiple groups and the device is realized.
Specifically, the moving mechanism includes a plurality of linear guide 15, be equipped with first linear motor 16 on the linear guide 15, the top of first linear motor 16 is equipped with slewing mechanism, one of them slewing mechanism with be located the below first arc connecting plate 12 links to each other, another slewing mechanism with be located the below second arc connecting plate 14 links to each other, first linear motor 16 with slewing mechanism all is connected with external power supply electricity, first linear motor 16 is connected with external signal, through linear guide 15, first linear motor 16 with cooperation between the slewing mechanism can be in proper order respectively sampled at the lily bamboo plant of same row, through slewing mechanism's rotation for can be to the slope direction of different lily bamboo plants suits, thereby can be better sample the work to lily bamboo, and the suitability is good, and can also carry out corresponding adjustment according to the specificity of different plants the position of enclosing mechanism.
Specifically, the rotating mechanism includes an arc guide rail 17 and a second linear motor 18, the arc guide rail 17 is fixedly connected with the top of the first linear motor 16, the second linear motor 18 is arranged on the arc guide rail 17, one of the second linear motors 18 is connected with the first arc connecting plate 12 located at the lowest position, the other second linear motor 18 is connected with the second arc connecting plate 14 located at the lowest position, the second linear motor 18 is electrically connected with an external power supply, the second linear motor 18 is connected with external signals, the second arc connecting plate 14 can rotate in the horizontal direction through the cooperation of the arc guide rail 17, the second linear motor 18 and the second arc connecting plate 14, and then can be adjusted to one side opposite to the plant inclination direction, and the first arc connecting plate 12 can be adjusted to one side opposite to the plant inclination direction through the cooperation of the arc guide rail 17, the second linear motor 18 and the first arc connecting plate 12, so that the second arc connecting plate 18 can be better matched with the natural plant growth state, and the subsequent work of the plant can be conveniently matched.
Specifically, be equipped with a plurality of first electric telescopic handle 19 between the linear guide 15, the flexible end of first electric telescopic handle 19 and one of them side linear guide 15 links to each other, the stiff end of first electric telescopic handle 19 with the opposite side linear guide 15 links to each other, first electric telescopic handle 19 is connected with external power supply electricity, first electric telescopic handle 19 is connected with external signal, through the effect of first electric telescopic handle 19, can be adjusted distance between the linear guide 15, and the suitability is strong to can be applicable to the basin of planting of different diameters lily bamboo and take a sample.
Specifically, the bottom of linear guide 15 is equipped with and is used for bearing the carrier 20 that lily bamboo cultivated in a pot, the bottom of linear guide 15 is equipped with slider 21, be equipped with spout 22 on the carrier 20, slider 21 is in slide in the spout 22, the bottom of carrier 20 is equipped with a plurality of bracing pieces 23, the bottom of bracing piece 23 is equipped with universal wheel 24, through slider 21 with cooperation between the spout 22 for linear guide 15 can carry out corresponding slip, is convenient for carry out corresponding regulation work.
Specifically, be equipped with the fixed plate 25 who links to each other with it on the arc guide rail 17, be equipped with backup pad 28 on the fixed plate 25, be equipped with the second electric telescopic handle 29 that links to each other with it on the backup pad 28, the flexible end of second electric telescopic handle 29 is equipped with one side open-ended casing 26, casing 26 with can adopt detachable mode to link together between the flexible end of second electric telescopic handle 29, for example through set up the draw-in groove on the surface of casing 26 and through the flexible end of second electric telescopic handle 29 inserts in this draw-in groove, opening one side of casing 26 with rectangle frame 6 is aligned, be equipped with the negative pressure pump 27 that is linked together with it on the casing 26, second electric telescopic handle 29 is connected with external power supply electricity, second electric telescopic handle 29 is connected with external signal, and can place the culture medium that is used for the group banks up in the casing 26, carry out tissue differentiation and follow-up cultivation work through the culture medium, through casing 26 with rectangle frame 6 contacts together, then through rectangle frame 26 the negative pressure is followed by the rectangle is drawn up the expansion end of second electric telescopic handle 29 and is moved to the rectangle frame 6 and is moved down, and can be moved to the rectangle frame 25 is followed by the rectangle and is moved to the follow-up, and is inhaled the rectangle frame is moved to the rectangle frame is followed by the rectangle frame is moved and is moved to the rectangle frame is followed by the tissue is moved to the rectangle frame is 25.
A use method of a lily bamboo tissue culture seedling cultivation device comprises the following steps of S1: the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4 enclose the branches of lily bulbs; s2: the position of the fourth arc-shaped water bag 4 is opposite to the direction of the growth inclination direction of the lily bamboo; s3: the cavities 5 in the first arc-shaped water bag 1, the second arc-shaped water bag 2, the third arc-shaped water bag 3 and the fourth arc-shaped water bag 4 are filled with water in an equivalent amount; s4: further filling a water source into the cavity 5 in the fourth arc-shaped water bag 4, so that the fourth arc-shaped water bag 4 continuously expands and presses the branches of the lily bulbs, and the branches of the lily bulbs are in a bending state; s5: the elastic blade 9 moves towards the branch direction of the lily bamboo after being extruded by the water filled bag body 9 until being cut and penetrated into the lily bamboo branch; s6: cutting the branches of the lily bulbs by using different elastic blades 9, and performing subsequent tissue culture on tissues cut from the branches of the lily bulbs after cutting.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.

Claims (7)

1. The lily bamboo tissue culture seedling cultivation device is characterized by comprising a plurality of groups of enclosing mechanisms for enclosing lily bamboo branches, wherein different enclosing mechanisms are connected together through a connecting mechanism, a moving mechanism is arranged on the connecting mechanism positioned at the lowest part, each enclosing mechanism comprises a first arc-shaped water bag, a second arc-shaped water bag, a third arc-shaped water bag and a fourth arc-shaped water bag, the first arc-shaped water bag, the second arc-shaped water bag and the third arc-shaped water bag are fixedly connected together, the fourth arc-shaped water bag is respectively connected with the second arc-shaped water bag and the third arc-shaped water bag, the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag enclose and close to form a ring shape, the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag are internally provided with a plurality of independent cavities, the fourth arc-shaped water bag is provided with a rectangular frame, the interior of the rectangular frame is provided with a plurality of hollow grooves, the end parts of the rectangular frame are provided with through grooves, the through grooves are mutually communicated, elastic blades which are abutted to the through grooves and can relatively move are arranged in the through grooves, a bag body is arranged in the hollow grooves, the bag body is connected with the non-cutting edge part of the elastic blades, the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag, the fourth arc-shaped water bag and the bag body are all communicated with an external water source, and the moving mechanism is electrically connected with an external power supply;
the connecting mechanism comprises a plurality of first connecting components and a plurality of second connecting components, the first connecting components comprise a first connecting rod and a plurality of first arc-shaped connecting plates, the end parts of the first connecting rods are connected with the first arc-shaped connecting plates, the first arc-shaped connecting plates are respectively connected with the first arc-shaped water bags, the second arc-shaped water bags and the third arc-shaped water bags, the second connecting components comprise a second connecting rod and a plurality of second arc-shaped connecting plates, the end parts of the second connecting rods are connected with the second arc-shaped connecting plates, and the second arc-shaped connecting plates are respectively connected with the fourth arc-shaped water bags;
the cultivation device is enabled to move to the cultivation position of the lily bulbs under the driving action of the moving mechanism, and the position of the fourth arc-shaped water bag is determined according to the growth inclination direction of the lily bulbs;
the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag enclose the branches of the lily bulbs;
the position of the fourth arc-shaped water bag is opposite to the direction of the growth inclination direction of the lily bulbs per se;
the cavities in the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag are filled with water in an equivalent amount, so that the branches of lily bulbs can be sufficiently straightened and fixed;
further filling a water source into the cavity in the fourth arc-shaped water bag, so that the fourth arc-shaped water bag is continuously expanded and extrudes the branches of the lily bulbs, and the branches of the lily bulbs are in a bending state;
the elastic blade moves towards the branch direction of the lily bamboo after being extruded by the water filled bag body until the elastic blade cuts open and goes deep into the lily bamboo branch;
cutting the branches of the lily bamboos through different elastic blades: the lily bamboo branches are inclined towards the other direction under the combined action of the second arc-shaped water bag and the fourth arc-shaped water bag by firstly filling water into the second arc-shaped water bag at one side, and then the bag bodies in the hollow groove at the same side of the second arc-shaped water bag in the vertical direction can be filled with water, so that the elastic blade moves towards the lily bamboo branches after being extruded by the bag bodies until the elastic blade cuts open and penetrates into the lily bamboo branches; then through the volume of releasing the water of second arc water pocket to the secondary before filling water and towards third arc water pocket fills water for lily bamboo branch is in third direction slope under the combined action of third arc water pocket and fourth arc water pocket, can be through being in vertical direction and with the same side of third arc water pocket the cell body in the hollow tank fills water this moment, and then makes the elastic blade is received after the extrusion of cell body moves towards lily bamboo branch direction until cut open and go deep into in the lily bamboo branch.
2. The lily bamboo tissue culture seedling cultivation device according to claim 1, wherein the moving mechanism comprises a plurality of linear guide rails, a first linear motor is arranged on the linear guide rails, a rotating mechanism is arranged at the top of the first linear motor, one rotating mechanism is connected with the first arc-shaped connecting plate located at the lowest position, the other rotating mechanism is connected with the second arc-shaped connecting plate located at the lowest position, the first linear motor and the rotating mechanism are electrically connected with an external power supply, and the first linear motor is connected with external signals.
3. The lily bamboo tissue culture seedling cultivation device according to claim 2, wherein the rotation mechanism comprises an arc-shaped guide rail and a second linear motor, the arc-shaped guide rail is fixedly connected with the top of the first linear motor, the second linear motor is arranged on the arc-shaped guide rail, one of the second linear motor is connected with the first arc-shaped connecting plate located at the lowest position, the other second linear motor is connected with the second arc-shaped connecting plate located at the lowest position, the second linear motor is electrically connected with an external power supply, and the second linear motor is in signal connection with the outside.
4. The lily bamboo tissue culture seedling cultivation device according to claim 2, wherein a plurality of first electric telescopic rods are arranged between the linear guide rails, the telescopic ends of the first electric telescopic rods are connected with the linear guide rails on one side of the linear guide rails, the fixed ends of the first electric telescopic rods are connected with the linear guide rails on the other side of the linear guide rails, the first electric telescopic rods are electrically connected with an external power supply, and the first electric telescopic rods are connected with external signals.
5. The lily bamboo tissue culture seedling cultivation device according to claim 2, wherein a bearing frame for bearing lily bamboo potting is arranged at the bottom of the linear guide rail, a sliding block is arranged at the bottom of the linear guide rail, a sliding groove is formed in the bearing frame, the sliding block slides in the sliding groove, a plurality of supporting rods are arranged at the bottom of the bearing frame, and universal wheels are arranged at the bottoms of the supporting rods.
6. The lily bamboo tissue culture seedling cultivating device according to claim 3, wherein a fixing plate connected with the arc-shaped guide rail is arranged on the arc-shaped guide rail, a supporting plate is arranged on the fixing plate, a second electric telescopic rod connected with the fixing plate is arranged on the supporting plate, a shell with an opening at one side is arranged at the telescopic end of the second electric telescopic rod, one side of the opening of the shell is aligned with the rectangular frame, a negative pressure pump communicated with the shell is arranged on the shell, the second electric telescopic rod is electrically connected with an external power supply, and the second electric telescopic rod is connected with an external signal.
7. The method for using the lily bamboo tissue culture seedling cultivation device according to claim 1, which is characterized in that,
s1: the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag enclose the branches of the lily bulbs;
s2: the position of the fourth arc-shaped water bag is opposite to the direction of the growth inclination direction of the lily bulbs per se;
s3: filling the cavities in the first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag with equal quantity;
s4: further filling a water source into the cavity in the fourth arc-shaped water bag, so that the fourth arc-shaped water bag is continuously expanded and extrudes the branches of the lily bulbs, and the branches of the lily bulbs are in a bending state;
s5: the elastic blade moves towards the branch direction of the lily bamboo after being extruded by the water filled bag body until the elastic blade cuts open and goes deep into the lily bamboo branch;
s6: cutting the branches of the lily bamboos through different elastic blades: the lily bamboo branches are inclined towards the other direction under the combined action of the second arc-shaped water bag and the fourth arc-shaped water bag by firstly filling water into the second arc-shaped water bag at one side, and then the bag bodies in the hollow groove at the same side of the second arc-shaped water bag in the vertical direction can be filled with water, so that the elastic blade moves towards the lily bamboo branches after being extruded by the bag bodies until the elastic blade cuts open and penetrates into the lily bamboo branches; then through the volume of releasing the water of second arc water pocket to the secondary before filling water and towards third arc water pocket fills water for lily bamboo branch is in third direction slope under the combined action of third arc water pocket and fourth arc water pocket, can be through being in vertical direction and with the same side of third arc water pocket the cell body in the hollow tank fills water this moment, and then makes the elastic blade is received after the extrusion of cell body moves towards lily bamboo branch direction until cut open and go deep into in the lily bamboo branch.
CN202210430567.7A 2022-04-23 2022-04-23 Lily bamboo tissue culture seedling cultivation device and application method thereof Active CN114766361B (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0819343A (en) * 1994-07-07 1996-01-23 Dainippon Seito Kk Divided inoculation of plant tissue fragments, device therefor and inoculation unit using the same
CN208798462U (en) * 2018-09-14 2019-04-30 海南天然橡胶产业集团股份有限公司 A kind of automatic intelligent rubber cutting machine
CN209283911U (en) * 2018-09-20 2019-08-23 海南煊烨生物科技有限公司 It is a kind of with electronic control function and be used for rubber tree full-automatic rubber cutting apparatus
CN112119713A (en) * 2020-09-23 2020-12-25 杭州仙珑宇盈科技有限公司 Device for planting dendrobium officinale on trunk
CN113196965A (en) * 2021-06-01 2021-08-03 缪静 Rapid shaping device and shaping method for pear tree grafting
CN113841522A (en) * 2021-10-29 2021-12-28 蒲县农业资源开发中心 Method for cultivating peach-shaped fruit trees
CN113924942A (en) * 2021-08-23 2022-01-14 吴�琳 Cutting device and cutting method
WO2022068254A1 (en) * 2020-09-30 2022-04-07 北京工业大学 Fully automatic intelligent rubber tapping robot

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0819343A (en) * 1994-07-07 1996-01-23 Dainippon Seito Kk Divided inoculation of plant tissue fragments, device therefor and inoculation unit using the same
CN208798462U (en) * 2018-09-14 2019-04-30 海南天然橡胶产业集团股份有限公司 A kind of automatic intelligent rubber cutting machine
CN209283911U (en) * 2018-09-20 2019-08-23 海南煊烨生物科技有限公司 It is a kind of with electronic control function and be used for rubber tree full-automatic rubber cutting apparatus
CN112119713A (en) * 2020-09-23 2020-12-25 杭州仙珑宇盈科技有限公司 Device for planting dendrobium officinale on trunk
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CN113196965A (en) * 2021-06-01 2021-08-03 缪静 Rapid shaping device and shaping method for pear tree grafting
CN113924942A (en) * 2021-08-23 2022-01-14 吴�琳 Cutting device and cutting method
CN113841522A (en) * 2021-10-29 2021-12-28 蒲县农业资源开发中心 Method for cultivating peach-shaped fruit trees

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