CN114711338A - Cat food attractant and preparation method thereof - Google Patents

Cat food attractant and preparation method thereof Download PDF

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Publication number
CN114711338A
CN114711338A CN202110321157.4A CN202110321157A CN114711338A CN 114711338 A CN114711338 A CN 114711338A CN 202110321157 A CN202110321157 A CN 202110321157A CN 114711338 A CN114711338 A CN 114711338A
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animal liver
parts
zymolyte
phagostimulant
cat food
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殷军
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Wing Biotech Shanghai Co ltd
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Wing Biotech Shanghai Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/40Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14Pretreatment of feeding-stuffs with enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)

Abstract

The invention relates to the field of pet phagostimulants, in particular to a cat food phagostimulant and a preparation method thereof, wherein the cat food phagostimulant comprises the following raw materials in parts by weight: 70-80 parts of Maillard reactants and 10-20 parts of auxiliary materials; the preparation raw materials of the Maillard reaction product comprise: 80-90 parts of animal liver zymolyte, 3-4 parts of monosaccharide, 1-3 parts of oligosaccharide and 0.5-5 parts of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 10-20 parts of animal liver and 0.03-1 part of protease. The cat phagostimulant is not influenced by pet food raw materials, has excellent cat food intake rate, and has less influence of the flavor on the environment.

Description

Cat food attractant and preparation method thereof
Technical Field
The invention relates to the field of pet phagostimulants, in particular to a cat food phagostimulant and a preparation method thereof.
Background
The phagostimulant is a flavor additive commonly used for animal feed and has the function of promoting the feed intake. With the increasing concern and career of the pet, whether the pet can eat healthily and satisfactorily becomes the focus of the attention of the pet owner, which leads to the particularly outstanding requirement of the pet food on the flavor, so the quality of the pet phagostimulant greatly influences the economic benefit of the pet food.
The pet attractant is a flavor substance capable of stimulating olfactory organ, gustatory organ and sensory nerve of the pet. According to the difference of pet species, living environment and physiological characteristics, different types of pet foods are combined to produce the phagostimulant with different forms. The main components of the pet attractant comprise pure natural plant extracts, animal liver extracts, spice, molasses and the like, and in order to ensure that the pet attractant has a good attractant effect on pets, the processing technology of the attractant is complex, meanwhile, the flavor of the pet attractant is greatly influenced in an open environment for a long time, and the attractant effect of pets is influenced after a period of time.
Disclosure of Invention
Aiming at some problems in the prior art, the invention provides a cat food attractant in a first aspect, which comprises the following raw materials in parts by weight: 70-80 parts of Maillard reactants and 10-20 parts of auxiliary materials; the preparation raw materials of the Maillard reactants comprise: 80-90 parts of animal liver zymolyte, 3-4 parts of monosaccharide, 1-3 parts of oligosaccharide and 0.5-5 parts of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 10-20 parts of animal liver and 0.03-1 part of protease.
As a preferable technical scheme of the invention, the auxiliary material is dextrin and/or gelatin.
In a preferred embodiment of the invention, the DE of the dextrin is from 10 to 15.
In a preferred embodiment of the invention, the gelatin has a Brookfield viscosity of 4 to 8mpa.s at 60 ℃.
In a preferred embodiment of the present invention, the oligosaccharide is isomaltooligosaccharide.
In a preferred embodiment of the present invention, the isomaltooligosaccharide is isomaltooligosaccharide-900 and/or isomaltooligosaccharide-500.
As a preferable technical scheme of the invention, the enzymolysis temperature of the protease is 30-50 ℃.
The second aspect of the invention provides a preparation method of the cat food attractant, which comprises the following steps:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 200-400 mesh sieve, standing at 25-40 deg.C for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 100-;
(3) preparing a phagostimulant: mixing Maillard reaction product and adjuvants, and stirring at constant temperature of 50-80 deg.C for 1-3h to obtain the final product.
As a preferred embodiment of the present invention, the step (1) includes: preparing animal liver zymolyte: crushing animal liver, sieving with 300-350 mesh sieve, standing at 25-30 deg.C for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte.
As a preferred embodiment of the present invention, the step (1) includes: preparing animal liver zymolyte: crushing animal liver, sieving with 300-350 mesh sieve, standing at 25-30 deg.C and 45-65% humidity for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte.
Compared with the prior art, the invention has the following beneficial effects:
(1) in the application, isomaltose hypgather-900 is used as a preparation raw material of the Maillard reaction product, and a good flavor effect can be obtained within a short Maillard reaction time;
(2) in the application, the crushed animal liver is subjected to enzymolysis after standing for 1-2h at the temperature of 25-30 ℃ and the humidity of 45-65%, and the influence of poor flavor caused by isomaltooligosaccharide can be avoided after subsequent Maillard reaction;
(3) the application adopts the combined action of gelatin with the Boehringer's viscosity of 4-8mpa.s at 60 ℃ and maltodextrin with the DE of 10-15, so that the flavor stability and the fragrance richness of the phagostimulant in an open-air environment with the humidity of more than 70 percent are improved;
(4) the crushed animal liver passes through a sieve of 250-;
(5) the application discloses phagostimulant for cats is not influenced by pet food raw materials and has excellent cat food intake rate.
Detailed Description
The present invention is illustrated by the following specific embodiments, but is not limited to the specific examples given below.
The invention provides a cat food attractant, which comprises the following raw materials in parts by weight: 70-80 parts of Maillard reactants and 10-20 parts of auxiliary materials; the preparation raw materials of the Maillard reaction product comprise: 80-90 parts of animal liver zymolyte, 3-4 parts of monosaccharide, 1-3 parts of oligosaccharide and 0.5-5 parts of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 10-20 parts of animal liver and 0.03-1 part of protease.
In one embodiment, the cat food attractant is prepared from the following raw materials in parts by weight: 75 parts of Maillard reactants and 15 parts of auxiliary materials; the preparation raw materials of the Maillard reactants comprise: 85 parts of animal liver zymolyte, 3.5 parts of monosaccharide, 2 parts of oligosaccharide and 3 parts of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 15 parts of animal liver and 0.5 part of protease.
The monosaccharide of the present invention is not particularly limited, and examples thereof include glucose, xylose, fructosyl galactose, and the like.
In one embodiment, the monosaccharide is xylose.
In one embodiment, the oligosaccharide is isomaltooligosaccharide.
Preferably, the isomaltooligosaccharide is isomaltooligosaccharide-900 and/or isomaltooligosaccharide-500; more preferably, the isomaltooligosaccharide is isomaltooligosaccharide-900.
Preferably, the oligosaccharide is isomaltooligosaccharide.
The applicant finds that in the preparation process of the Maillard reactant of the application, the conventional monosaccharide is used as the preparation raw material of the Maillard reactant, and in the preparation process of the Maillard reactant, when the reaction is carried out at 110 ℃ in the temperature range of 100-. The applicant considers that the possible reason is that in the preparation process of the animal liver zymolyte, the animal liver is crushed and then stands for 1-2 hours in the environment with the temperature of 25-30 ℃ and the humidity of 45-65% for enzymolysis reaction, when the animal liver zymolyte obtained under the condition participates in Maillard reaction with isomaltooligosaccharide at the same time, because glucose molecules in isomaltose are linked through alpha-1, 6 glycosidic bonds, the conformation structure of the structure weakens the acting force between Maillard reactant molecules, so that the Maillard reactant reaction is accelerated, meanwhile, when the obtained animal liver zymolyte reacts, the reaction with the isomaltooligosaccharide is probably slow, the quick reactants of the amino acid and the monosaccharide are gathered around the isomaltooligosaccharide, and finally the obtained phagostimulant is eaten by the cat, has various flavors, and greatly reduces the influence of poor flavor caused by isomaltooligosaccharide.
In one embodiment, the excipient is dextrin and/or gelatin.
Preferably, the DE of the dextrin is 10-15; more preferably, the dextrin has a DE of 14.
The DE value is the percentage of reducing sugars (calculated as glucose) on dry matter of the syrup.
Preferably, the dextrin is selected from one or more of maltodextrin, cyclodextrin, white dextrin and yellow dextrin; more preferably, the dextrin is maltodextrin.
Maltodextrin is a low conversion product between starch and starch sugar, and is abbreviated as MD in English. An amorphous powder which is white or slightly yellowish in appearance, free from visible impurities to the naked eye, has a special odor and is not sweet or slightly sweet in taste. Has wide application, and in 1970, Ueberbacaher defines maltodextrin as follows: the product which takes starch as raw material and has the DE value controlled below 20% by hydrolysis is called maltodextrin. The definition in the united states is: the carbohydrate product is obtained by hydrolyzing, converting and spray drying the corn starch raw material. The definition of China is: starch or starchiness is used as raw material, and the starch derivative without free starch is prepared by enzymatic low-degree hydrolysis, refining and spray drying. The series products have the following common product specifications according to different DE values: MD10, MD15, MD20, and the like.
The physical properties of maltodextrin are directly related to the DE value of its hydrolysis, so that the DE value not only indicates the degree of hydrolysis, but also is an important indicator for mastering the characteristics of the product. Generally speaking, the higher the hydrolysis degree of maltodextrin, the larger the DE value, the larger the oxygen solubility, sweetness, permeability, fermentability, browning reaction and freezing point depression of the product; the poorer the organization, viscosity, pigment stability and crystallization resistance.
Preferably, the gelatin has a Boehringer's viscosity of 4 to 8mpa.s at 60 ℃; more preferably, the gelatin has a Boehringer's viscosity of 6mpa.s at 60 ℃.
The Bosch viscosity of gelatin in this application is 12.5 wt% aqueous gelatin solution measured using ND-2 Bosch viscometer.
In one embodiment, the excipients are dextrin and gelatin.
Preferably, the weight ratio of dextrin to gelatin is (1-2.5): 1; more preferably, the weight ratio of dextrin to gelatin is 1.8: 1.
the applicant finds that the addition of isomaltooligosaccharide leads to a phagostimulant with greatly increased speed of flavor bloom reduction when the phagostimulant exists in an open air environment with humidity of more than 70%, which greatly reduces the use value of the phagostimulant, and further, the applicant surprisingly finds that when the auxiliary materials comprise dextrin and gelatin, the dextrin is maltodextrin, especially DE is 10-15, and the gelatin has a Brookfield viscosity of 4-8mpa.s at 60 ℃, the flavor stability of the phagostimulant in the environment with humidity of more than 70% can be obviously improved, the applicant considers that the possible reason is that the maltodextrin with hydrolysis degree under the condition and the Brookfield viscosity of 4-8mpa.s can effectively generate action force with Maillard reactant molecules when being mixed with the Maillard reactant, the binding force between the molecules is increased, and the water molecules in the air are effectively prevented from entering the phagostimulant, maintaining flavor stability in an open air environment with humidity of more than 70% for a long time.
In one embodiment, the amino acid is selected from one or more of glycine, cysteine, methionine, glutamic acid.
The amino acid is not particularly limited in the present application, and those skilled in the art can select the amino acid according to the description of the present application.
In one embodiment, the animal liver is poultry liver.
Preferably, the poultry liver is chicken liver.
The protease is low-temperature flavor enzyme, is purchased from Novoxil (China) investment Limited company and has the model of NPF-10500.
The second aspect of the invention provides a preparation method of the cat food attractant, which comprises the following steps:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 200-400 mesh sieve, standing at 25-40 deg.C for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 100-;
(3) preparing a phagostimulant: mixing Maillard reaction product and adjuvants, and stirring at constant temperature of 50-80 deg.C for 1-3h to obtain the final product.
In the preparation process of the cat food attractant, the animal liver zymolyte is prepared by the preparation raw material of the animal liver zymolyte, if the amount of the obtained animal liver zymolyte is not enough to participate in the next reaction, repeating the steps until the amount of the obtained animal liver zymolyte reaches the usage amount of the animal liver zymolyte in the preparation step of the Maillard reaction product in the next step, taking the corresponding weight part of the animal liver zymolyte to participate in the preparation of the Maillard reaction product, and if the amount of the Maillard reaction product obtained in the step is not enough to participate in the next step, repeating the preparation process of the Maillard reactants until the amount of the obtained Maillard reactants reaches the use amount of the Maillard reactants in the preparation process of the phagostimulant in the next step, and finally taking corresponding parts by weight of the Maillard reactants to participate in the preparation of the cat food phagostimulant.
In one embodiment, the step (1) comprises: preparing animal liver zymolyte: crushing animal liver, sieving with 300-350 mesh sieve, standing at 25-30 deg.C for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte.
According to the application, the crushed animal liver passes through a sieve of 250-400 meshes, and the phagostimulant prepared from the product obtained by the method has strong fragrance under the conditions of no addition of distilled water and stirring in the enzymolysis reaction process.
Preferably, the step (1) includes: crushing animal liver, sieving with 300-350 mesh sieve, standing at 25-30 deg.C and 45-65% humidity for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte.
Further preferably, the step (1) includes: crushing animal liver, sieving with 300-350 mesh sieve, standing at 25-30 deg.C and 45-65% humidity for 1-2 hr, adding protease, stirring for 1-5min, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte.
More preferably, the step (1) comprises: crushing animal liver, sieving with 320 mesh sieve, standing at 26 deg.C and 50% humidity for 1.5 hr, adding protease, stirring for 3min, and reacting at 35 deg.C for 60min to obtain animal liver zymolyte.
In one embodiment, the step (2) comprises: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 102 deg.C for 0.8h to obtain Maillard reaction product.
In one embodiment, the step (3) comprises: mixing the Maillard reaction product and the auxiliary materials, and stirring at the constant temperature of 60 ℃ for 1.2h to obtain the catalyst.
Examples
Hereinafter, the present invention will be described in more detail by way of examples, but it should be understood that these examples are merely illustrative and not restrictive. The starting materials used in the examples which follow are all commercially available unless otherwise stated.
Example 1
The embodiment 1 of the invention provides a cat food attractant which comprises the following raw materials in parts by weight: 70 parts of Maillard reactants and 10 parts of auxiliary materials; the preparation raw materials of the Maillard reactants comprise: 80 parts of animal liver zymolyte, 3 parts of monosaccharide, 1 part of oligosaccharide and 0.5 part of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 10 parts of animal liver and 0.03 part of protease.
The protease is low-temperature flavor enzyme, is purchased from Novoxil (China) investment Limited company and has the model of NPF-10500.
The animal liver is chicken liver.
The amino acid is glycine and cysteine, and the weight ratio of the glycine to the cysteine is 1: 1.
the oligosaccharide is isomaltooligosaccharide-900, which is purchased from Yong-Ningbao bio-products Co., Ltd.
The monosaccharide is xylose.
The auxiliary materials are maltodextrin and gelatin, and the weight ratio is 1: 1; the DE of the maltodextrin is 10, and is purchased from Zhengzhou Tianzhui starch product company Limited in the second seven regions of Zhengzhou city, Henan province; the gelatin had a Bosch viscosity of 6mPa.s at 60 ℃ and was purchased from Biotechnology Ltd, Hainan, Western.
The preparation method of the cat food phagostimulant comprises the following steps:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 320 mesh sieve, standing at 26 deg.C and 50% humidity for 1.5 hr, adding protease, stirring for 3min, and reacting at 35 deg.C for 60min to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 102 deg.C for 0.8h to obtain Maillard reaction product;
(3) preparing a phagostimulant: mixing the Maillard reaction product and auxiliary materials, and stirring at the constant temperature of 60 ℃ for 1.2 h.
Example 2
The embodiment 2 of the invention provides a cat food phagostimulant which is prepared from the following raw materials in parts by weight: 80 parts of Maillard reactants and 20 parts of auxiliary materials; the preparation raw materials of the Maillard reaction product comprise: 90 parts of animal liver zymolyte, 4 parts of monosaccharide, 3 parts of oligosaccharide and 5 parts of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 20 parts of animal liver and 1 part of protease.
The protease is low-temperature flavor enzyme, is purchased from Novoxil (China) investment Limited company and has the model of NPF-10500.
The animal liver is chicken liver.
The amino acid is glycine and cysteine, and the weight ratio is 1: 1.
the oligosaccharide is isomaltooligosaccharide-900, which is purchased from Yongzhao biological GmbH.
The monosaccharide is xylose.
The auxiliary materials are maltodextrin and gelatin, and the weight ratio is 2.5: 1; the DE of the maltodextrin is 15, and the maltodextrin is purchased from Zhengzhou Tianzhu starch products Limited company in two seven regions of Zhengzhou city of Henan province; the gelatin had a Bosch viscosity of 6mPa.s at 60 ℃ and was purchased from Biotechnology Ltd, Hainan, Western.
The preparation method of the cat food attractant comprises the following steps:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 320 mesh sieve, standing at 26 deg.C and 50% humidity for 1.5 hr, adding protease, stirring for 3min, and reacting at 35 deg.C for 60min to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 102 deg.C for 0.8h to obtain Maillard reaction product;
(3) preparing a phagostimulant: mixing the Maillard reaction product and auxiliary materials, and stirring at the constant temperature of 60 ℃ for 1.2 h.
Example 3
The embodiment 3 of the invention provides a cat food phagostimulant which is prepared from the following raw materials in parts by weight: 75 parts of Maillard reactants and 15 parts of auxiliary materials; the preparation raw materials of the Maillard reactants comprise: 85 parts of animal liver zymolyte, 3.5 parts of monosaccharide, 2 parts of oligosaccharide and 3 parts of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 15 parts of animal liver and 0.5 part of protease.
The protease is low-temperature flavor enzyme, is purchased from Novoxil (China) investment Limited company and has the model of NPF-10500.
The animal liver is chicken liver.
The amino acid is glycine and cysteine, and the weight ratio of the glycine to the cysteine is 1: 1.
the oligosaccharide is isomaltooligosaccharide-900, which is purchased from Yongzhao biological GmbH.
The monosaccharide is xylose.
The auxiliary materials are maltodextrin and gelatin, and the weight ratio is 1.8: 1; the maltodextrin has a DE of 14 and is purchased from Zhengzhou Tianzhui starch product Co., Ltd in Zhengzhou province in Erseven areas of Zhengzhou province, Henan province; the gelatin had a Bosch viscosity of 6mPa.s at 60 ℃ and was purchased from Biotechnology Ltd, Hainan, Western.
The preparation method of the cat food attractant comprises the following steps:
(1) preparing animal liver zymolyte: crushing animal livers, sieving with a 320-mesh sieve, standing for 1.5h in an environment with the temperature of 26 ℃ and the humidity of 50%, adding protease, stirring for 3min, and reacting at the constant temperature of 35 ℃ for 60min to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 102 deg.C for 0.8h to obtain Maillard reaction product;
(3) preparing a phagostimulant: mixing the Maillard reaction product and auxiliary materials, and stirring at the constant temperature of 60 ℃ for 1.2 h.
Example 4
The embodiment 4 of the invention provides the cat food phagostimulant, and the specific implementation mode of the cat food phagostimulant is the same as that of the embodiment 3, except that the oligosaccharide is replaced by maltose.
The specific implementation mode of the preparation method of the cat food phagostimulant is the same as that of the example 3.
Example 5
Example 5 of the present invention provides a cat food phagostimulant in the same manner as in example 3.
The preparation method of the cat food phagostimulant comprises the following specific steps:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 320 mesh sieve, standing at 26 deg.C for 1.5 hr, adding protease, stirring for 3min, and reacting at 35 deg.C for 60min to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 102 deg.C for 0.8h to obtain Maillard reaction product;
(3) preparing a phagostimulant: mixing Maillard reactants and adjuvants, and stirring at constant temperature of 60 deg.C for 1.2 h.
Example 6
Example 6 of the present invention provides the cat food phagostimulant, which is prepared in a specific embodiment in the same manner as example 3, except that the auxiliary material is gelatin, has a brix viscosity of 6mpa.s at 60 ℃, and is purchased from bioscience, ltd.
The specific implementation mode of the preparation method of the cat food phagostimulant is the same as that of the example 3.
Example 7
Example 7 of the present invention provides the cat food phagostimulant, which is specifically embodied in the same manner as in example 3, except that the supplemental material is maltodextrin having a DE of 14, which is purchased from zheng tianzhu starch products limited, zheng town, two seven areas of zheng city, hou, southern he.
The specific implementation mode of the preparation method of the cat food phagostimulant is the same as that of the example 3.
Example 8
The embodiment 8 of the invention provides the cat food phagostimulant, and the specific implementation mode is the same as that in the embodiment 3, except that the auxiliary materials are maltodextrin and gelatin, and the weight ratio of the auxiliary materials to the food attractant is 1.8: 1; the maltodextrin has a DE of 19 and is purchased from Zhengzhou Tianzhui starch product Co., Ltd in the second seven regions of Zhengzhou city, Henan province; the gelatin had a Boehringer viscosity at 60 ℃ of 6mPa.s, available from Biotechnology, Inc., of Hao, Xianhao.
The specific implementation mode of the preparation method of the cat food phagostimulant is the same as that in example 3.
Example 9
Example 9 of the present invention provides a cat food phagostimulant in the same manner as in example 3.
The preparation method of the cat food phagostimulant comprises the following specific steps:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 320 mesh sieve, standing at 26 deg.C and 30% humidity for 1.5 hr, adding protease, stirring for 3min, and reacting at 35 deg.C for 60min to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 102 deg.C for 0.8h to obtain Maillard reaction product;
(3) preparing a phagostimulant: mixing the Maillard reaction product and auxiliary materials, and stirring at the constant temperature of 60 ℃ for 1.2 h.
Example 10
Example 10 of the present invention provides a cat food phagostimulant in the same manner as in example 3.
The preparation method of the cat food phagostimulant comprises the following specific steps:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 320 mesh sieve, standing at 26 deg.C and 80% humidity for 1.5 hr, adding protease, stirring for 3min, and reacting at 35 deg.C for 60min to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 102 deg.C for 0.8h to obtain Maillard reaction product;
(3) preparing a phagostimulant: mixing the Maillard reaction product and auxiliary materials, and stirring at the constant temperature of 60 ℃ for 1.2 h.
Performance evaluation
1. The feed intake rate is as follows: 10 pet cats aged 2-6 years old with good health condition, equivalent weight and various breeds are selected, wherein the sex ratio of the pet cats is 1: 1.
The baits obtained in the examples 1 to 10 were continuously fed for 10 days by bernas pure cat food purchased from shanghai nemacy pet products ltd, respectively, and each cat was fed independently without eating other foods during the feeding period. The pet cat feed is characterized in that a feeding basin is provided for each pet cat, 1 meal is fed every day, each meal lasts for 12 hours, the pet cat feeds the pet cat with the same amount every day, the feeding basin needs to be cleaned before each feeding, sufficient drinking water is prepared, tableware is fixed to prevent overturning, the position of the feeding basin is exchanged when the pet cat feeds the pet cat at a next meal, and grains fed by each meal cannot be reused. The feed intake rate for each pet cat was recorded daily, i.e. (total amount of cat food provided-cat food remaining)/total amount of cat food provided 100%. After 10 days, the average feed intake per cat was calculated and the results are shown in table 1. Wherein, after completion of the one example test, the 10 pet cats were subjected to the next example test.
2. Flavor sensory test: the odor of the phagostimulants obtained in examples 1-10 were recorded separately and classified into 3 grades, class a: the meat flavor is obvious, the sauce flavor is strong, and the fragrance is strong; b stage: the meat flavor is obvious, the sauce flavor is thin, and the foreign flavor is obvious; c level: the meat flavor was not obvious, no sauce flavor was present, the aroma was not obvious, the miscellaneous flavor was severe, and the results are shown in table 1.
3. Flavor stability: the phagostimulants obtained in examples 1 to 10 were placed in an open air environment with 80% humidity for 1 week, and then the feeding rates were measured again according to the above-mentioned methods for measuring feeding rates, and the results are shown in table 1.
TABLE 1
Figure BDA0002992889080000111
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims (10)

1. The cat food attractant is characterized by comprising the following preparation raw materials in parts by weight: 70-80 parts of Maillard reactants and 10-20 parts of auxiliary materials; the preparation raw materials of the Maillard reactants comprise: 80-90 parts of animal liver zymolyte, 3-4 parts of monosaccharide, 1-3 parts of oligosaccharide and 0.5-5 parts of amino acid; the preparation raw materials of the animal liver zymolyte comprise: 10-20 parts of animal liver and 0.03-1 part of protease.
2. The cat food phagostimulant of claim 1 wherein the excipient is dextrin and/or gelatin.
3. The cat food phagostimulant of claim 2 wherein the dextrin has a DE of 10 to 15.
4. The cat food phagostimulant of claim 2 wherein the gelatin has a boeh viscosity of 4 to 8mpa.s at 60 ℃.
5. The cat food phagostimulant of claim 1 wherein the oligosaccharide is isomaltooligosaccharide.
6. The cat food phagostimulant according to claim 5, wherein the isomaltooligosaccharide is isomaltooligosaccharide-900 and/or isomaltooligosaccharide-500.
7. The cat food phagostimulant of any one of claims 1 to 6 wherein the phagostimulant is characterized in that the enzymatic hydrolysis temperature of the protease is from 20 ℃ to 50 ℃.
8. A method of preparing a cat food attractant as defined in any one of claims 1 to 7 including the steps of:
(1) preparing animal liver zymolyte: crushing animal liver, sieving with 200-400 mesh sieve, standing at 25-40 deg.C for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte;
(2) preparation of maillard reactants: mixing animal liver zymolyte, monosaccharide, oligosaccharide and amino acid, and reacting at 100-;
(3) preparing a phagostimulant: mixing Maillard reaction product and adjuvants, and stirring at constant temperature of 50-80 deg.C for 1-3h to obtain the final product.
9. The method for preparing the phagostimulant according to claim 8, wherein the step (1) comprises the following steps: preparing animal liver zymolyte: crushing animal liver, sieving with a sieve of 300-350 meshes, standing at 25-30 ℃ for 1-2h, adding protease, and reacting at 20-50 ℃ for 20min-2h to obtain the animal liver zymolyte.
10. The method for preparing the phagostimulant according to claim 9, wherein the step (1) comprises the following steps: preparing animal liver zymolyte: crushing animal liver, sieving with 300-350 mesh sieve, standing at 25-30 deg.C and 45-65% humidity for 1-2 hr, adding protease, and reacting at 20-50 deg.C for 20min-2 hr to obtain animal liver zymolyte.
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