CN114561316A - Lactobacillus chaff-like bacteria and application thereof - Google Patents
Lactobacillus chaff-like bacteria and application thereof Download PDFInfo
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- 241000186660 Lactobacillus Species 0.000 title claims abstract description 47
- 229940039696 lactobacillus Drugs 0.000 title claims abstract description 47
- 241000894006 Bacteria Species 0.000 title claims abstract description 33
- 239000010802 sludge Substances 0.000 claims abstract description 79
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 74
- 238000012545 processing Methods 0.000 claims abstract description 48
- 239000000463 material Substances 0.000 claims abstract description 40
- 239000010865 sewage Substances 0.000 claims abstract description 40
- 238000000855 fermentation Methods 0.000 claims abstract description 38
- 230000004151 fermentation Effects 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 32
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 30
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000002781 deodorant agent Substances 0.000 claims abstract description 24
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical group O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000002156 mixing Methods 0.000 claims abstract description 21
- 238000004321 preservation Methods 0.000 claims abstract description 19
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 15
- 239000004310 lactic acid Substances 0.000 claims abstract description 15
- 238000009264 composting Methods 0.000 claims abstract description 13
- 238000005507 spraying Methods 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 9
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- 230000008569 process Effects 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 9
- 239000002351 wastewater Substances 0.000 claims description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- 244000063299 Bacillus subtilis Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 241000831743 Lactobacillus parafarraginis Species 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims description 6
- 239000011574 phosphorus Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 5
- 229910052785 arsenic Inorganic materials 0.000 claims description 5
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 claims description 5
- 229910052793 cadmium Inorganic materials 0.000 claims description 5
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 5
- 229910052804 chromium Inorganic materials 0.000 claims description 5
- 239000011651 chromium Substances 0.000 claims description 5
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 5
- 229910052753 mercury Inorganic materials 0.000 claims description 5
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 4
- 244000234623 Coprinus comatus Species 0.000 claims description 3
- 235000004439 Coprinus comatus Nutrition 0.000 claims description 3
- 240000001462 Pleurotus ostreatus Species 0.000 claims description 3
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims description 3
- 238000003672 processing method Methods 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 description 20
- 244000005700 microbiome Species 0.000 description 9
- 239000002689 soil Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
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- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 108700029181 Bacteria lipase activator Proteins 0.000 description 2
- 241000207925 Leonurus Species 0.000 description 2
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 235000010855 food raising agent Nutrition 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- HGUFODBRKLSHSI-UHFFFAOYSA-N 2,3,7,8-tetrachloro-dibenzo-p-dioxin Chemical compound O1C2=CC(Cl)=C(Cl)C=C2OC2=C1C=C(Cl)C(Cl)=C2 HGUFODBRKLSHSI-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241000334160 Isatis Species 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
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- 229910052799 carbon Inorganic materials 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Fertilizers (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention relates to the technical field of sludge resource utilization, in particular to lactobacillus chaff and application thereof. The invention provides the lactobacillus chaff-like bacteria with the preservation number of CGMCC No.23951, and provides the lactobacillus chaff-like bacteria which can be used for treating the residual sludge of the sewage in the grain processing so as to convert the residual sludge into the biological organic fertilizer. The method comprises the steps of mixing and stirring the residual sludge of the grain processing sewage, mushroom fungus residues and furfural residues to obtain a mixed material; carrying out aerobic composting fermentation, and spraying a deodorant in the fermentation process to obtain an organic fertilizer; mixing the organic fertilizer with lactic acid bacteria to obtain an organic fertilizer with the pH of 6.5-7.5; mixing with functional strains, and culturing under anoxic condition to obtain bio-organic fertilizer; the lactobacillus is a kind of chaff lactobacillus with the preservation number of CGMCC No. 23951. The obtained biological organic fertilizer has better performance.
Description
Technical Field
The invention relates to the technical field of sludge resource utilization, in particular to lactobacillus chaff and application thereof.
Background
China is an ancient agricultural kingdom, and organic fertilizers occupy an extremely important position in Chinese traditional agricultural production. The history and reality of traditional agriculture relying on applying organic fertilizers to obtain high-yield and high-quality agricultural products provide rich experience for the development of world agriculture, but the history related to commercial organic fertilizers is only a short decade. The fertilizer is applied in a large amount in agricultural production, so that the yield of agricultural products is greatly improved, but adverse effects such as quality reduction of the agricultural products, soil hardening, water source pollution and the like are caused, the content of beneficial microorganisms in soil organic matters, soil nutrients and soil can be effectively improved by additionally applying the organic fertilizer and the biological organic fertilizer, and nutrient elements such as nitrogen, phosphorus, potassium and the like fixed in the soil can be released by utilizing the microorganisms, so that the using amount of the fertilizer is reduced.
At present, the organic fertilizer and the bio-organic fertilizer are produced by mainly using livestock manure, mainly because the livestock manure is easy to obtain, but the fermentation is incomplete in the fermentation process, the crop seedling burning condition is often caused after the fertilizer is applied, and meanwhile, the heavy metal in the livestock manure can cause soil pollution.
The sludge disposal method still mainly uses incineration, the sludge incineration method heats and dries dehydrated sludge by using an incinerator, and then oxidizes organic matters in the sludge at high temperature to make the sludge become a small amount of ash, the incineration cost is 180 yuan/ton, the cost is too high, and great pressure is brought to the production and operation of a sewage treatment plant. Direct incineration of sludge without drying is not only very difficult, but also extremely uneconomical in terms of energy consumption. Large investment in treatment facilities, high treatment cost, high maintenance cost of equipment and generation of dioxin, a strong carcinogen.
The sludge landfill method has the advantages of simple and feasible treatment method, low cost, no need of high dehydration of the sludge and strong adaptability. However, sludge landfills also have problems, particularly with respect to landfill leachate and gas formation. Leachate is a severely contaminated liquid that can contaminate groundwater environments if landfills are located or improperly operated. The gas produced by landfills is primarily methane, which can cause explosion and combustion if appropriate measures are taken.
Disclosure of Invention
In view of the above, the technical problem to be solved by the present invention is to provide a lactobacillus chaff-like bacterium and an application thereof, and the lactobacillus chaff-like bacterium provided by the present invention can be used for treating excess sludge of sewage from grain processing, so that the excess sludge can be converted into a bio-organic fertilizer.
The invention provides a kind of Lactobacillus chaff (Lactobacillus parafarraginis), which is characterized in that the preservation number is CGMCC No. 23951.
The invention also provides an application of the lactobacillus chaff-like bacteria in treatment of residual sludge of sewage in grain processing.
The invention also provides application of the lactobacillus chaff-like bacteria as a pH value regulator or a deodorant for treating the residual sludge of the grain processing sewage.
The invention also provides a method for treating the excess sludge of the grain processing sewage, which comprises the following steps:
A) mixing and stirring the residual sludge of the grain processing sewage, mushroom fungus residues and furfural residues to obtain a mixed material;
B) carrying out aerobic composting fermentation on the mixed material, and spraying a deodorant in the fermentation process to obtain an organic fertilizer;
C) mixing the organic fertilizer with lactic acid bacteria to obtain a mixed organic fertilizer with the pH value of 6.5-7.5;
D) mixing the mixed organic fertilizer with functional strains, and culturing under an anoxic condition to obtain a biological organic fertilizer;
the lactobacillus is a kind of chaff lactobacillus with the preservation number of CGMCC No. 23951.
Preferably, in the step A), in the dry matter of the excess sludge of the grain processing sewage, the content of total arsenic is less than or equal to 15mg/kg, the content of total cadmium is less than or equal to 3mg/kg, the content of total lead is less than or equal to 50mg/kg, the content of total chromium is less than or equal to 150mg/kg, and the content of total mercury is less than or equal to 2 mg/kg;
the water content of the residual sludge of the grain processing sewage is 85-90%, and the pH value is 6.5-7;
in the dry matter of the residual sludge of the grain processing sewage, the content of organic matters is 45-50 wt%, the content of nitrogen is 1.4-1.7 wt%, the content of phosphorus is 3-4 wt%, and the content of potassium is 0.8-1.2 wt%.
Preferably, in the step A), the mushroom dregs comprise at least one of needle mushroom dregs, oyster mushroom dregs and coprinus comatus dregs;
the particle size of the mushroom dreg is 2-3 cm;
the particle size of the furfural residues is 2-3 cm.
Preferably, in the step A), the carbon-nitrogen ratio in the mixed material is 15-35: 1;
the water content of the mixed material is 50-70%.
Preferably, in the step A), the addition amount of the mushroom dregs is 30-200% of the mass of the residual sludge of the grain processing sewage;
the addition amount of the furfural residues is 20-100% of the mass of the residual sludge of the grain processing sewage.
Preferably, in the step B), the aerobic composting fermentation temperature is 40-75 ℃, the humidity is 50-70%, and the time is 15-30 d;
the deodorant sprayed in the fermentation process specifically comprises the following steps:
turning and throwing are carried out in the fermentation process, and deodorant is sprayed in the turning and throwing process.
Preferably, in the step D), the addition amount of the functional strain is 0.2-0.3% of the mass of the mixed organic fertilizer;
the functional strain is bacillus subtilis;
the temperature for culturing under the anoxic condition is room temperature, and the time is 15-20 days.
The invention provides the lactobacillus chaff-like bacteria with the preservation number of CGMCC No.23951, and provides the lactobacillus chaff-like bacteria which can be used for treating the residual sludge of the sewage in the grain processing so as to convert the residual sludge into the biological organic fertilizer. The method comprises the steps of mixing and stirring the residual sludge of the grain processing sewage, mushroom fungus residues and furfural residues to obtain a mixed material; carrying out aerobic composting fermentation, and spraying a deodorant in the fermentation process to obtain an organic fertilizer; mixing the organic fertilizer with lactic acid bacteria to obtain an organic fertilizer with the pH of 6.5-7.5; mixing with functional strains, and culturing under anoxic condition to obtain a biological organic fertilizer; the lactobacillus is a kind of chaff lactobacillus with the preservation number of CGMCC No. 23951. The obtained biological organic fertilizer has better performance.
The invention can treat the excess sludge of the grain processing sewage, not only can meet the requirement of environmental management, but also can meet the resource utilization, and simultaneously solves the problems of high energy consumption in the incineration process of high sludge water content or secondary pollution to the environment in the sludge landfill process.
Biological preservation Instructions
Biomaterial LBSW21-02, categorical nomenclature: lactobacillus chaff-like Lactobacillus parafarraginis, deposited at CGMCC (CGMCC) by 11 months and 22 days 2021, with the addresses as follows: the microbial research institute of China academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, and the preservation number is CGMCC No. 23951.
Detailed Description
The technical solutions of the present invention will be described clearly and completely below with reference to embodiments of the present invention, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a kind of Lactobacillus chaff (Lactobacillus parafarraginis), which is characterized in that the preservation number is CGMCC No. 23951.
The invention screens and obtains a microbial strain which can produce lactic acid under the anoxic condition from the soybean whey wastewater, the strain is named as LBS W21-02, the propagation speed is high, the tolerance is strong, and tests show that the strain can be applied to the fermentation of organic fertilizers and reduce the pH value of the organic fertilizers. 16SrDNA sequencing was performed on the strain LBSW21-02 and the determined 16SrDNA sequences were BLAST aligned. The morphological characteristics of the strain are as follows: the colony diameter is about 3mm, and is convex, round, smooth, fine and white in surface. Through identification, the Lactobacillus chaff-like bacteria (Lactobacillus parafarraginis) is determined on the molecular level, and the preservation number of the Lactobacillus chaff-like bacteria preserved in the China general microbiological culture Collection center is CGMCC No. 23951.
The invention also provides application of the lactobacillus chaff-like bacteria in treatment of residual sludge of grain processing sewage.
The invention also provides application of the lactobacillus chaff-like bacteria as a pH value regulator or a deodorant for treating the residual sludge of the grain processing sewage.
The invention also provides a method for treating the excess sludge of the grain processing sewage, which comprises the following steps:
A) mixing and stirring the residual sludge of the grain processing sewage, mushroom fungus residues and furfural residues to obtain a mixed material;
B) carrying out aerobic composting fermentation on the mixed material, and spraying a deodorant in the fermentation process to obtain an organic fertilizer;
C) mixing the organic fertilizer with lactic acid bacteria to obtain a mixed organic fertilizer with the pH value of 6.5-7.5;
D) mixing the mixed organic fertilizer with functional strains, and culturing under an anoxic condition to obtain a biological organic fertilizer;
the lactobacillus is a kind of chaff lactobacillus with the preservation number of CGMCC No. 23951.
The method comprises the steps of mixing and stirring the residual sludge of the grain processing sewage, the mushroom fungus residues and the furfural residues to obtain a mixed material.
In some embodiments of the invention, the content of total arsenic is less than or equal to 15mg/kg, the content of total cadmium is less than or equal to 3mg/kg, the content of total lead is less than or equal to 50mg/kg, the content of total chromium is less than or equal to 150mg/kg, and the content of total mercury is less than or equal to 2mg/kg in dry matter of the excess sludge of the grain processing sewage. In certain embodiments, the grain processing wastewater excess sludge has a dry matter content of 4.3mg/kg total arsenic, 2.1mg/kg total cadmium, 20.5mg/kg total lead, 30.33mg/kg total chromium, and 0.11mg/kg total mercury.
In some embodiments of the invention, the water content of the residual sludge of the grain processing sewage is 85% -90%, and the pH value is 6.5-7. In some embodiments, the grain processing wastewater excess sludge has a water content of 89.5% and a pH of 7.
In some embodiments of the invention, the dry matter of the grain processing sewage excess sludge contains 45 wt% to 50 wt% of organic matter, 1.4 wt% to 1.7 wt% of nitrogen, 3 wt% to 4 wt% of phosphorus, and 0.8 wt% to 1.2 wt% of potassium. In certain embodiments, the grain processing sewage excess sludge has a dry matter content of 46.5 wt% organic matter, 1.6 wt% nitrogen, 3.7 wt% phosphorus, and 1.1 wt% potassium.
In certain embodiments of the invention, the grain processing wastewater excess sludge is sludge sediment obtained after settling of grain processing wastewater.
In certain embodiments of the invention, the mushroom dregs comprise at least one of needle mushroom dregs, oyster mushroom dregs, and coprinus comatus dregs. In some embodiments of the invention, the grain size of the mushroom dregs is 2-3 cm. The source of the mushroom dregs is not particularly limited in the present invention, and the mushroom dregs may be generally commercially available.
In certain embodiments of the invention, the furfural residue refers primarily to post-corn cob processing material. In certain embodiments of the invention, the particle size of the furfural residue is 2-3 cm. The source of the furfural residue is not particularly limited, and the furfural residue can be generally sold in the market.
In some embodiments of the invention, the mixing and stirring are performed in a preparation machine, and the mixing and stirring are performed on the sludge, the mushroom fungus residues and the furfural residues, so that the efficiency of the material during fermentation is improved, and the phenomenon of uneven fermentation is avoided.
In some embodiments of the invention, the carbon-nitrogen ratio in the mixed material is 15-35: 1; the water content of the mixed material is 50-70%. In certain embodiments, the carbon to nitrogen ratio in the mixed feed is 20: 1; the water content of the mixed material is 70%.
In some embodiments of the invention, the addition amount of the mushroom dregs is 30-200% of the mass of the residual sludge of the grain processing sewage. In certain embodiments, the mushroom dregs are added in an amount of 50% of the mass of the residual sludge of the grain processing sewage.
In some embodiments of the invention, the furfural residue is added in an amount of 20-100% by mass of the residual sludge of the grain processing sewage. In certain embodiments, the furfural residue is added in an amount of 50% of the mass of the residual sludge of the grain processing wastewater.
And (3) after obtaining a mixed material, carrying out aerobic composting fermentation on the mixed material, and spraying a deodorant in the fermentation process to obtain the organic fertilizer.
In some embodiments of the invention, the pH value of the organic fertilizer is 8.5-9.2. In certain embodiments, the pH of the organic fertilizer is 8.78.
In some embodiments of the invention, before the aerobic composting fermentation of the mixed material, the method further comprises:
and piling the mixed materials into a long ridge stack.
In some embodiments of the invention, the length of the long ridge stack is 20-50 m, the height is 1.5-2.5 m, and the width is 2-3 m. In some embodiments, the stacks of strips have a length of 20m, a height of 2m and a width of 2.5 m.
In some embodiments of the invention, the deodorant sprayed during fermentation is specifically:
turning and throwing are carried out in the fermentation process, and deodorant is sprayed in the turning and throwing process.
In the invention, the temperature and oxygen in the aerobic composting fermentation process are controlled by controlling the turner to turn over materials, and in some embodiments, the fermentation process is turned over 2 times per day.
In certain embodiments of the invention, the turning is performed in a turner. The turner can be a turner produced by Shandong Longtai stockbreeding machinery Limited. During the turning process, the material is taken up by the roller rotating at high speed and moves backwards for a certain distance. Therefore, in the turning process, the turning machine can break up and crush the materials and take away part of water. The operation can be implemented in an open place and a workshop greenhouse. The material is broken through turning the material, and meanwhile, oxygen can enter the material during turning, so that fermentation is accelerated, and odor is reduced.
In certain embodiments of the present invention, the odor control agent is a biological odor control agent, including lactic acid bacteria or commercially available odor control agents. Specifically, the deodorant may be Lactobacillus chaff-like bacteria (Lactobacillus parafarraginis) with the preservation number of CGMCC No.23951, or Lactobacillus produced by leonurus bio-science ltd.c. of Qingdao or a Wanjiefen microbial deodorant produced by Shaanxi Fuan bio-science ltd.c. of Isatis. In this process, the lactic acid bacteria act as a deodorant. Therefore, the invention requests to protect the application of the lactobacillus chaff as a deodorant for treating the residual sludge of the grain processing sewage.
The beneficial microorganisms in the biological deodorant can rapidly capture and adsorb and decompose malodorous gas through self growth and metabolism and metabolites thereof, and the biological deodorant is a nontoxic, pollution-free and ecologically balanced deodorant. 0.1 kg of deodorant is used for each cubic meter of mixed material, and the mixed material is diluted by 30-50 times and sprayed on the mixed material. In certain embodiments, the dilution is by a factor of 40. Spraying is carried out during each turning and throwing, and a spraying device carried on the turning and throwing machine is utilized for spraying.
In some embodiments of the invention, the aerobic composting fermentation temperature is 40-75 ℃, the humidity is 50-70%, and the time is 15-30 days. In certain embodiments, the aerobic composting fermentation is at a temperature of 60 ℃ or 55 ℃ and a humidity of 60% for a period of 20 days.
And after obtaining the organic fertilizer, mixing the organic fertilizer with lactic acid bacteria to obtain a mixed organic fertilizer with the pH value of 6.5-7.5.
In the invention, the lactobacillus is the lactobacillus chaff-like bacteria with the preservation number of CGMCC No. 23951. In this process, the lactic acid bacteria act as a pH regulator. Therefore, the invention requests to protect the application of the lactobacillus chaff as a pH value regulator for treating the residual sludge of the grain processing sewage.
In certain embodiments of the invention, the pH of the mixed organic fertilizer is 7.22, 7.14 or 6.95.
And mixing the mixed organic fertilizer with functional strains, and culturing under an anoxic condition to obtain the biological organic fertilizer.
In some embodiments of the present invention, the functional strain is bacillus subtilis, and specifically may be bacillus subtilis produced by the company of leonurus glauca bioltd.
In some embodiments of the invention, the addition amount of the functional strain is 0.2-0.3% of the mass of the mixed organic fertilizer. In certain embodiments, the addition amount of the functional strain is 0.2% of the mass of the mixed organic fertilizer.
In some embodiments of the invention, the temperature of the culture under the anoxic condition is room temperature, and the time is 15-20 days. In certain embodiments, the temperature of the incubation under hypoxic conditions is room temperature for 15 days.
In some embodiments of the present invention, after the culturing, the method further comprises: and crushing and sieving the obtained organic fertilizer. In certain embodiments, the size of the particles after pulverization is 2mm or less. In certain embodiments, a 6 mesh screen is screened.
The method utilizes residual microorganisms in the mushroom dregs as fermentation strains, and the sludge contains a large amount of organic matters and nutrients which can be used as nutrients of the microorganisms to enable the microorganisms to be rapidly propagated and metabolized, so that the high temperature generated in the process can destroy the cell walls of zoogloea in the sludge to achieve the effect of breaking the walls, thereby reducing the water content of the sludge and degrading harmful substances in the sludge to enable the harmful substances to become organic fertilizers, and realizing the resource utilization.
According to the invention, mushroom residues are used as fermentation auxiliary materials, so that the high water content of sludge is neutralized, the water content of the mixed material is ensured to meet the fermentation requirement, and meanwhile, the rest strains in the mushroom residues are used as a leavening agent to reduce the leavening agent investment and reduce the fertilizer production cost. The mushroom dregs are used as production fertilizer to ferment the fertilizer, thereby realizing comprehensive utilization of resources.
According to the invention, the furfural residues are added, so that the C/N of the obtained mixed material is 15-35: 1, promoting the reproduction and metabolism of microorganisms.
According to the invention, the pH value of the compost is adjusted by using the lactic acid bacteria, the pH value of the fermented organic fertilizer is ensured to be 6.5-7.5, the fertilizer is prevented from becoming alkaline after fermentation is finished, and the production standard of the biological organic fertilizer is met. After the functional strains are added, the fertilizer is stably planted in the fertilizer under the anoxic condition for 15 days.
The invention degrades the sludge through biotransformation, partial heavy metals in the sludge are degraded by the adsorption of microorganisms, and the high temperature (55-70 ℃) generated by the metabolism of the microorganisms can kill worm eggs and grass seeds in the sludge, thereby thoroughly carrying out harmless treatment on the sludge.
The source of the raw materials used in the present invention is not particularly limited, and the raw materials may be those generally commercially available.
In order to further illustrate the present invention, the following will describe the method for treating excess sludge of grain processing sewage in detail with reference to the examples, but it should not be construed as limiting the scope of the present invention.
Example 1
The residual sludge of the grain processing sewage is sludge sediment obtained after the grain processing sewage is settled, wherein in dry matters of the sludge sediment, the content of total arsenic is 4.3mg/kg, the content of total cadmium is 2.1mg/kg, the content of total lead is 20.5mg/kg, the content of total chromium is 30.33mg/kg, and the content of total mercury is 0.11 mg/kg; in the dry matter of the sludge sediment, the content of organic matters is 46.5 wt%, the content of nitrogen is 1.6 wt%, the content of phosphorus is 3.7 wt%, and the content of potassium is 1.1 wt%; the water content of the residual sludge is 89.5%, and the pH value is 7.
The mushroom residues are commercially available needle mushroom residues, and the particle size of the particles is 2-3 cm.
The particle size of the furfural residues is 2-3 cm, and the furfural residues are generally sold in the market.
The method for treating the excess sludge of the grain processing sewage comprises the following steps:
1) putting 3 tons of the excess sludge into a preparation machine, adding mushroom residues, wherein the adding amount of the mushroom residues is 50% of the weight of the sludge, adding furfural residues, and the adding amount of the furfural residues is 50% of the weight of the sludge, and then mixing and stirring by the preparation machine to obtain a mixed material; in the mixed material, the carbon-nitrogen ratio is 20: 1; the water content of the mixed material is 70%;
2) piling the mixed materials into a long strip ridge stack, wherein the length of the long strip ridge stack is 20m, the height of the long strip ridge stack is 2m, and the width of the long strip ridge stack is 2.5m, carrying out aerobic composting fermentation, turning over in the fermentation process, turning over 2 times every day, spraying lactobacillus (lactobacillus chaff-like bacteria with the preservation number of CGMCC No.23951, 0.1 kg of lactobacillus is used for each cubic meter of the mixed materials, diluting by 40 times and spraying on the mixed materials) along with a turner in the turning over process, keeping the temperature and humidity of the ridge stack at 60 ℃ in the fermentation process, and obtaining an organic fertilizer with the pH value of 8.78 after fermenting for 20 days;
3) adding lactobacillus (Lactobacillus chaff-like bacteria with the preservation number of CGMCC No. 23951) into the organic fertilizer to obtain a mixed organic fertilizer, wherein the pH value of the mixed organic fertilizer is 7.22;
4) and (2) adding bacillus subtilis (produced by Ulva sylvestris Vahl Co., Ltd.) in the mixed organic fertilizer obtained in the step 3), wherein the addition amount of the bacillus subtilis is 0.2% of the mass of the mixed organic fertilizer, culturing for 15 days at room temperature under the anoxic condition, crushing, and sieving with a 6-mesh sieve to obtain the biological organic fertilizer.
According to the bioorganic fertilizer execution standard (NY 884-.
The bio-organic fertilizer has no odor, and the odor threshold is 0.
Example 2
Changing the adding amount of lactobacillus (the lactobacillus chaff-like bacteria with the preservation number of CGMCC No. 23951) in the step 3) of the embodiment 1 to ensure that the pH value of the obtained mixed organic fertilizer is 7.14; the rest steps and parameters are carried out according to the steps of the example 1, and the biological organic fertilizer is prepared.
According to the bioorganic fertilizer execution standard (NY 884-.
The bio-organic fertilizer has no odor, and the odor threshold is 0.
Example 3
Changing the adding amount of lactobacillus (the lactobacillus chaff-like bacteria with the preservation number of CGMCC No. 23951) in the step 3) of the embodiment 1 to ensure that the pH value of the obtained mixed organic fertilizer is 6.95; the remaining steps and parameters were performed as in example 1 to produce a bio-organic fertilizer.
According to the bioorganic fertilizer execution standard (NY 884-.
The bio-organic fertilizer has no odor, and the odor threshold is 0.
Example 4
The deodorant in example 1 was replaced with lactic acid bacteria produced by Ulva Neurosa Vahl, Inc., and the remaining steps and parameters were performed according to the procedure in example 1, to obtain a bio-organic fertilizer.
According to the bioorganic fertilizer execution standard (NY 884-.
The odor of the bio-organic fertilizer can be obviously detected, and the smell threshold is 3.
Example 5
The lactic acid bacteria in the step 2) of the embodiment 1 are replaced by Wanjiefen (produced by Shaanxi Fuan Biotechnology Co., Ltd.), and the rest steps and parameters are carried out according to the steps of the embodiment 1, so as to prepare the biological organic fertilizer.
According to the bioorganic fertilizer execution standard (NY 884-.
The bio-organic fertilizer has strong odor, and the odor threshold is 4.
Comparative example 1
The lactic acid bacteria in step 3) of example 1 were replaced with commercially available lactic acid bacteria (manufactured by sienga and biotechnology limited) so that the pH of the resulting mixed organic fertilizer was 7.82; the rest steps and parameters are carried out according to the steps of the example 1, and the biological organic fertilizer is prepared.
According to the biological organic fertilizer execution standard (NY 884-2012), the detection of the obtained biological organic fertilizer shows that the effective viable count of the biological organic fertilizer is 0.94 hundred million/g, and the organic matter content is 45.3 wt%.
The bio-organic fertilizer has no odor, and the odor threshold is 0.
The experimental result shows that the biological organic fertilizer obtained by the invention is detected according to the biological organic fertilizer execution standard (NY 884-.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. Lactobacillus chaff-like bacteria (Lactobacillus parafarraginis) is characterized in that the preservation number is CGMCC No. 23951.
2. The use of the lactobacillus chaffeensis of claim 1 for the treatment of excess sludge from grain processing wastewater.
3. Use of the lactobacillus chaffeensis of claim 1 as a pH regulator or deodorant for the treatment of excess sludge from grain processing wastewater.
4. A method for treating excess sludge of grain processing sewage comprises the following steps:
A) mixing and stirring the residual sludge of the grain processing sewage, mushroom fungus residues and furfural residues to obtain a mixed material;
B) carrying out aerobic composting fermentation on the mixed material, and spraying a deodorant in the fermentation process to obtain an organic fertilizer;
C) mixing the organic fertilizer with lactic acid bacteria to obtain a mixed organic fertilizer with the pH value of 6.5-7.5;
D) mixing the mixed organic fertilizer with functional strains, and culturing under an anoxic condition to obtain a biological organic fertilizer;
the lactobacillus is a kind of chaff lactobacillus with the preservation number of CGMCC No. 23951.
5. The treatment method according to claim 4, wherein in step A), the content of total arsenic is less than or equal to 15mg/kg, the content of total cadmium is less than or equal to 3mg/kg, the content of total lead is less than or equal to 50mg/kg, the content of total chromium is less than or equal to 150mg/kg, and the content of total mercury is less than or equal to 2mg/kg in dry matter of the excess sludge of the grain processing wastewater;
the water content of the residual sludge of the grain processing sewage is 85-90%, and the pH value is 6.5-7;
in the dry matter of the residual sludge of the grain processing sewage, the content of organic matters is 45-50 wt%, the content of nitrogen is 1.4-1.7 wt%, the content of phosphorus is 3-4 wt%, and the content of potassium is 0.8-1.2 wt%.
6. The processing method according to claim 4, wherein in the step A), the mushroom dregs comprise at least one of needle mushroom dregs, oyster mushroom dregs and coprinus comatus dregs;
the particle size of the mushroom dreg is 2-3 cm;
the particle size of the furfural residues is 2-3 cm.
7. The treatment method according to claim 4, wherein in the step A), the carbon-nitrogen ratio in the mixed material is 15-35: 1;
the water content of the mixed material is 50-70%.
8. The treatment method according to claim 4, wherein in the step A), the addition amount of the mushroom dregs is 30-200% of the mass of the residual sludge of the grain processing sewage;
the addition amount of the furfural residues is 20-100% of the mass of the residual sludge of the grain processing sewage.
9. The treatment method according to claim 4, wherein in the step B), the aerobic composting fermentation temperature is 40-75 ℃, the humidity is 50-70%, and the time is 15-30 d;
the deodorant sprayed in the fermentation process specifically comprises the following steps:
turning and throwing are carried out in the fermentation process, and deodorant is sprayed in the turning and throwing process.
10. The treatment method as claimed in claim 4, wherein in the step D), the addition amount of the functional strain is 0.2-0.3% of the mass of the mixed organic fertilizer;
the functional strain is bacillus subtilis;
the temperature for culturing under the anoxic condition is room temperature, and the time is 15-20 days.
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| CN116218727A (en) * | 2023-02-10 | 2023-06-06 | 青岛尚德生物技术有限公司 | A kind of microbial agent and its application of improving the aerobic fermentation rate of livestock and poultry manure |
| CN117016290A (en) * | 2023-09-01 | 2023-11-10 | 辽宁省微生物科学研究院 | Earthing material for Dictyophora rubrovalvata cultivation and preparation method and application thereof |
| CN121450548A (en) * | 2025-12-31 | 2026-02-03 | 浙江森井生物技术股份有限公司 | Lactobacillus glutamicum LF-20 and application thereof |
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| US20190300407A1 (en) * | 2016-10-25 | 2019-10-03 | Kunshan Huake Institute Of Biopolymer Co., Ltd | Biological Floc and Uses Thereof, for example, in Sludge Innocent Treatment and Industrial Water Purification Treatment |
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| CN103242069A (en) * | 2013-05-29 | 2013-08-14 | 冯国恒 | Sludge resource utilization method |
| US20190300407A1 (en) * | 2016-10-25 | 2019-10-03 | Kunshan Huake Institute Of Biopolymer Co., Ltd | Biological Floc and Uses Thereof, for example, in Sludge Innocent Treatment and Industrial Water Purification Treatment |
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| CN117016290A (en) * | 2023-09-01 | 2023-11-10 | 辽宁省微生物科学研究院 | Earthing material for Dictyophora rubrovalvata cultivation and preparation method and application thereof |
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