CN114525204A - Cell biology experiment culture apparatus - Google Patents

Cell biology experiment culture apparatus Download PDF

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Publication number
CN114525204A
CN114525204A CN202210170190.6A CN202210170190A CN114525204A CN 114525204 A CN114525204 A CN 114525204A CN 202210170190 A CN202210170190 A CN 202210170190A CN 114525204 A CN114525204 A CN 114525204A
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box
fixedly connected
cell biology
culture
main body
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杨川
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West China Hospital of Sichuan University
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West China Hospital of Sichuan University
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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Abstract

The invention discloses a cell biology experiment culture device, and relates to the technical field of cell culture. The invention comprises a main body device, a control device, a fresh air device, an illumination sterilization device and a constant temperature device, wherein the main body device is arranged on a laboratory bench, and the control device is arranged on the front side of the main body device. The novel culture device for cell biology experiments is improved by arranging the main body device, the operation is simple, the use is convenient, the intelligent degree is high, the temperature and the humidity in the culture box can be controlled by arranging the control device, a plurality of different types of cell culture can be carried out simultaneously, the air fluidity in the culture box can be ensured and the stability of a sterile environment can be maintained by arranging the fresh air device, the illumination intensity in the culture box can be ensured by arranging the illumination sterilization device, the sterilization effect can be carried out by utilizing ultraviolet rays, the temperature in the culture box can be stabilized to be always at the temperature required by cells by arranging the constant temperature device, the fluctuation is extremely small, and the noise is extremely small.

Description

Cell biology experiment culture apparatus
Technical Field
The invention belongs to the technical field of cell culture, and particularly relates to a cell biology experiment culture device.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. For cell culture, a constant-temperature and sterile culture environment is very important, and most of the existing constant-temperature culture boxes can achieve the constant-temperature and sterile culture environment, but the existing constant-temperature culture boxes have certain defects when being used, for example, the existing constant-temperature culture boxes are not high in use efficiency, when workers need to culture different types of cells, the cells need to be cultured respectively, so that the culture period is long, the work efficiency is low, and in addition, the required conditions are different when different cells are cultured, and the common constant-temperature culture boxes are difficult to meet. Therefore, we propose a culture device for cell biology experiments.
Disclosure of Invention
The invention aims to provide a cell biology experiment culture device, which solves the existing problems: the current constant temperature incubator availability factor is not high, when the staff need cultivate different kind cells, needs to cultivate respectively, leads to cultivateing the cycle length, and work efficiency is low to when cultivateing different cells, required condition is also different, and general constant temperature incubator is difficult to satisfy.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a cell biology experiment culture device, which comprises a main body device, a control device, a fresh air device, an illumination sterilization device and a constant temperature device, wherein the main body device is arranged on an experiment table, the control device is arranged on the front side of the main body device, the fresh air device is arranged on the top of the main body device, the illumination sterilization device is arranged inside the main body device, and the constant temperature device is arranged inside the main body device.
Further, the main body device comprises a base, universal wheels, a machine cabin, a box body, a box door, an observation window and a partition plate groove, wherein the base is arranged at the top of the universal wheels, the base is fixedly connected with the universal wheels, the universal wheels are fixedly arranged on the experiment table, the machine cabin is arranged at the top of the base, the machine cabin is fixedly connected with the base, the box body is arranged at the top of the machine cabin, the box body is fixedly connected with the machine cabin, the box door is arranged on the front side of the box body, the box door is fixedly connected with the box body, the observation window is arranged on the front side of the box door, the observation window is fixedly connected with the box door, the partition plate groove is arranged in the box body, and the partition plate groove is fixedly connected with the box body.
Further, controlling means is including control mainboard, display screen, action column, the control mainboard sets up the front in quick-witted storehouse, control mainboard and quick-witted storehouse fixed connection, the display screen sets up the front at the control mainboard, display screen and control mainboard fixed connection, the action column sets up the side at the display screen, action column and display screen fixed connection.
Further, the new trend device includes ventilation pipe, new trend filter screen, fan, glass interlayer, intelligent valve, control cabinet, the ventilation pipe sets up the top at the box, ventilation pipe and box fixed connection, the new trend filter screen sets up the inside at the ventilation pipe, new trend filter screen and ventilation pipe fixed connection, the fan sets up the inside at the ventilation pipe, fan and ventilation pipe fixed connection, the glass interlayer sets up the inside at the box, glass interlayer and box fixed connection, the intelligent valve sets up the top at the glass interlayer, intelligent valve and glass interlayer fixed connection, the control cabinet sets up the top at the intelligent valve, control cabinet and intelligent valve fixed connection.
Further, state illumination sterilizing equipment and include degerming ultraviolet lamp, illumination LED lamp, temperature monitoring probe, degerming ultraviolet lamp sets up the inside top surface at the box, degerming ultraviolet lamp and box fixed connection, illumination LED lamp sets up the side at degerming ultraviolet lamp, illumination LED lamp and degerming ultraviolet lamp fixed connection, temperature monitoring probe sets up the inside at the box, temperature monitoring probe and box fixed connection.
Further, constant temperature equipment includes compressor unit, exchange wind channel, hot air exitus, compressor unit sets up the inside in quick-witted storehouse, compressor unit and quick-witted storehouse fixed connection, the setting of exchange wind channel is at the back in quick-witted storehouse and extends to the inside in quick-witted storehouse, exchange wind channel and quick-witted storehouse fixed connection, hot air exitus sets up the top at compressor unit, hot air exitus and compressor unit fixed connection.
The invention has the following beneficial effects:
1. the novel culture device for cell biology experiments is improved by arranging the main body device, the operation is simple, the use is convenient, the intelligent degree is high, the temperature and the humidity in the incubator can be controlled by arranging the control device, a plurality of different types of cell culture can be carried out simultaneously, and the air mobility in the incubator and the stability of sterile environment can be ensured by arranging the fresh air device.
2. The illumination sterilization device can ensure the illumination intensity in the incubator and perform sterilization by using ultraviolet rays, and the constant temperature device can stabilize the temperature in the incubator to be always at the temperature required by cells, so that the fluctuation is extremely small, and the noise is extremely small.
Of course, it is not necessary for any product to practice the invention to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a rear view of the present invention;
FIG. 3 is an internal structural view of the present invention;
FIG. 4 is an enlarged view of a portion of the present invention at A;
fig. 5 is a top view of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a main body device; 101. a base; 102. a universal wheel; 103. a machine cabin; 104. a box body; 105. a box door; 106. an observation window; 107. a partition plate groove; 2. a control device; 201. a control main board; 202. a display screen; 203. an operating column; 3. a fresh air device; 301. a vent pipe; 302. a fresh air filter screen; 303. a fan; 304. a glass barrier layer; 305. an intelligent valve; 306. a control seat; 4. an illumination sterilization device; 401. sterilizing ultraviolet lamps; 402. an illuminating LED lamp; 403. a temperature monitoring probe; 5. a thermostatic device; 501. a compressor unit; 502. exchanging air channels; 503. and a hot air outlet.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention is a cell biology experiment culture apparatus, including a main body device 1, a control device 2, a fresh air device 3, an illumination sterilization device 4, and a constant temperature device 5, wherein the main body device 1 is disposed on an experiment table, the control device 2 is disposed on the front surface of the main body device 1, the fresh air device 3 is disposed on the top of the main body device 1, the illumination sterilization device 4 is disposed inside the main body device 1, and the constant temperature device 5 is disposed inside the main body device 1.
The main body device 1 comprises a base 101, a universal wheel 102, a machine cabin 103, a box body 104, a box door 105, an observation window 106 and a partition groove 107, wherein the base 101 is arranged at the top of the universal wheel 102, the base 101 is fixedly connected with the universal wheel 102, the universal wheel 102 is fixedly arranged on a laboratory bench, the machine cabin 103 is arranged at the top of the base 101, the machine cabin 103 is fixedly connected with the base 101, the box body 104 is arranged at the top of the machine cabin 103, the box body 104 is fixedly connected with the machine cabin 103, the box door 105 is arranged at the front of the box body 104, the box door 105 is fixedly connected with the box body 104, the observation window 106 is arranged at the front of the box door 105, the observation window 106 is fixedly connected with the box door 105, the partition groove 107 is arranged inside the box body 104, the partition groove 107 is fixedly connected with the box body 104, and a novel culture device for cytological experiments can be improved by arranging the base 101, the universal wheel 102, the machine cabin 103, the box body 104, the box door 105, the observation window 106 and the partition groove 107 in the main body device 1, simple operation, convenient use and high intelligence degree.
Controlling means 2 is including control mainboard 201, display screen 202, action column 203, control mainboard 201 sets up the front at machine storehouse 103, control mainboard 201 and machine storehouse 103 fixed connection, display screen 202 sets up the front at control mainboard 201, display screen 202 and control mainboard 201 fixed connection, action column 203 sets up the side at display screen 202, action column 203 and display screen 202 fixed connection, through setting up the control mainboard 201 among controlling means 2, display screen 202, action column 203 can control the incubator interior humiture and can carry out the cell culture of a plurality of different grade types simultaneously.
The fresh air device 3 comprises a ventilation pipe 301, a fresh air filter screen 302, a fan 303, a glass interlayer 304, an intelligent valve 305 and a control seat 306, the ventilation pipe 301 is arranged at the top of the box body 104, the ventilation pipe 301 is fixedly connected with the box body 104, the fresh air filter screen 302 is arranged inside the ventilation pipe 301, the fresh air filter screen 302 is fixedly connected with the ventilation pipe 301, the fan 303 is arranged inside the ventilation pipe 301, the fan 303 is fixedly connected with the ventilation pipe 301, the glass interlayer 304 is arranged inside the box body 104, the glass interlayer 304 is fixedly connected with the box body 104, the intelligent valve 305 is arranged at the top of the glass interlayer 304, the intelligent valve 305 is fixedly connected with the glass interlayer 304, the control seat 306 is arranged at the top of the intelligent valve 305, and the control seat 306 is fixedly connected with the intelligent valve 305, the ventilation pipe 301, the fresh air filter screen 302, the fan 303, the glass interlayer 304, the intelligent valve 305 and the control seat 306 in the fresh air device 3 are arranged to ensure the air fluidity in the incubator and maintain the stability of the sterile environment.
Illumination sterilizing equipment 4 includes degerming ultraviolet lamp 401, illumination LED lamp 402, temperature monitoring probe 403, degerming ultraviolet lamp 401 sets up the inside top surface at box 104, degerming ultraviolet lamp 401 and box 104 fixed connection, illumination LED lamp 402 sets up the side at degerming ultraviolet lamp 401, illumination LED lamp 402 and degerming ultraviolet lamp 401 fixed connection, temperature monitoring probe 403 sets up the inside at box 104, temperature monitoring probe 403 and box 104 fixed connection, through setting up degerming ultraviolet lamp 401 in the illumination sterilizing equipment 4, illumination LED lamp 402, temperature monitoring probe 403 can guarantee the illumination intensity in the incubator and utilize the ultraviolet ray to carry out bactericidal action.
The thermostatic device 5 comprises a compressor unit 501, an exchange air duct 502 and a hot air outlet 503, wherein the compressor unit 501 is arranged inside the machine cabin 103, the compressor unit 501 is fixedly connected with the machine cabin 103, the exchange air duct 502 is arranged on the back of the machine cabin 103 and extends to the inside of the machine cabin 103, the exchange air duct 502 is fixedly connected with the machine cabin 103, the hot air outlet 503 is arranged at the top of the compressor unit 501, the hot air outlet 503 is fixedly connected with the compressor unit 501, the temperature in the incubator can be stabilized by the compressor unit 501, the exchange air duct 502 and the hot air outlet 503 which are arranged in the thermostatic device 5 and is always at the temperature required by cells, the fluctuation is extremely small, and the noise is extremely small.
One specific application of this embodiment is: after the invention is put into use, a user uses the novel culture device for cell biology experiments through the base 101, the universal wheel 102, the machine cabin 103, the box body 104, the box door 105, the observation window 106 and the partition plate groove 107 in the main body device 1, the operation is simple, the use is convenient, the intelligence degree is high, the temperature and the humidity in the culture box can be controlled through the control mainboard 201, the display screen 202 and the operation column 203 in the control device 2, a plurality of cells of different types can be cultured simultaneously, the air fluidity in the culture box and the sterile environment stability can be ensured through the ventilation pipe 301, the fresh air filter screen 302, the fan 303, the glass partition layer 304, the intelligent valve 305 and the control seat 306 in the fresh air device 3, the illumination intensity in the culture box can be ensured through the sterilizing ultraviolet lamp 401, the illuminating LED lamp 402 and the temperature monitoring probe 403 in the illuminating and sterilizing device 4 and the sterilization effect can be realized by utilizing ultraviolet rays, the compressor unit 501, the exchange air duct 502 and the hot air outlet 503 in the thermostatic device 5 can stabilize the temperature in the incubator to be always at the temperature required by the cells, the fluctuation is extremely small, and the noise is extremely small.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (10)

1. The utility model provides a cell biology experiment culture apparatus, includes main part device (1), controlling means (2), new trend device (3), illumination sterilizing equipment (4), constant temperature equipment (5), its characterized in that: the main body device (1) is arranged on a laboratory bench, the control device (2) is arranged on the front face of the main body device (1), the fresh air device (3) is arranged at the top of the main body device (1), the illumination and sterilization device (4) is arranged inside the main body device (1), and the constant temperature device (5) is arranged inside the main body device (1).
2. The culture device for cell biology experiments according to claim 1, wherein: the main body device (1) comprises a base (101), universal wheels (102), a machine cabin (103), a box body (104), a box door (105), an observation window (106) and a partition plate groove (107), wherein the base (101) is arranged at the top of the universal wheels (102), the base (101) is fixedly connected with the universal wheels (102), the universal wheels (102) are fixedly arranged on an experiment table, the machine cabin (103) is arranged at the top of the base (101), and the machine cabin (103) is fixedly connected with the base (101).
3. The culture device for cell biology experiment according to claim 2, wherein: the box body (104) is arranged at the top of the machine bin (103), the box body (104) is fixedly connected with the machine bin (103), the box door (105) is arranged on the front surface of the box body (104), the box door (105) is fixedly connected with the box body (104), the observation window (106) is arranged on the front surface of the box door (105), the observation window (106) is fixedly connected with the box door (105), the partition plate groove (107) is arranged inside the box body (104), and the partition plate groove (107) is fixedly connected with the box body (104).
4. The culture device for cell biology experiments according to claim 1, wherein: control device (2) is including control mainboard (201), display screen (202), action column (203), control mainboard (201) sets up the front in machine storehouse (103), control mainboard (201) and machine storehouse (103) fixed connection, display screen (202) set up the front in control mainboard (201), display screen (202) and control mainboard (201) fixed connection, action column (203) set up the side in display screen (202), action column (203) and display screen (202) fixed connection.
5. The culture device for cell biology experiments according to claim 1, wherein: fresh air device (3) include ventilation pipe (301), new trend filter screen (302), fan (303), glass interlayer (304), intelligent valve (305), control seat (306), ventilation pipe (301) set up the top at box (104), ventilation pipe (301) and box (104) fixed connection, new trend filter screen (302) set up the inside at ventilation pipe (301), new trend filter screen (302) and ventilation pipe (301) fixed connection.
6. The culture device for cell biology experiments according to claim 5, wherein: the glass partition wall type air conditioner is characterized in that the fan (303) is arranged inside the ventilation pipe (301), the fan (303) is fixedly connected with the ventilation pipe (301), the glass partition wall (304) is arranged inside the box body (104), and the glass partition wall (304) is fixedly connected with the box body (104).
7. The culture device for cell biology experiments according to claim 5, wherein: the intelligent valve (305) is arranged on the top of the glass isolation layer (304), the intelligent valve (305) is fixedly connected with the glass isolation layer (304), the control seat (306) is arranged on the top of the intelligent valve (305), and the control seat (306) is fixedly connected with the intelligent valve (305).
8. The culture device for cell biology experiments according to claim 1, wherein: illumination sterilizing equipment (4) are including degerming ultraviolet lamp (401), illumination LED lamp (402), temperature monitoring probe (403), degerming ultraviolet lamp (401) set up the inside top surface at box (104), degerming ultraviolet lamp (401) and box (104) fixed connection, illumination LED lamp (402) set up the side at degerming ultraviolet lamp (401), illumination LED lamp (402) and degerming ultraviolet lamp (401) fixed connection, temperature monitoring probe (403) set up the inside at box (104), temperature monitoring probe (403) and box (104) fixed connection.
9. The culture device for cell biology experiments according to claim 1, wherein: the constant temperature device (5) comprises a compressor unit (501), an exchange air duct (502) and a hot air outlet (503), wherein the compressor unit (501) is arranged inside the machine cabin (103), and the compressor unit (501) is fixedly connected with the machine cabin (103).
10. The culture device for cell biology experiment according to claim 9, wherein: the exchange air duct (502) is arranged on the back of the machine bin (103) and extends to the interior of the machine bin (103), the exchange air duct (502) is fixedly connected with the machine bin (103), the hot air outlet (503) is arranged at the top of the compressor unit (501), and the hot air outlet (503) is fixedly connected with the compressor unit (501).
CN202210170190.6A 2022-02-23 2022-02-23 Cell biology experiment culture apparatus Pending CN114525204A (en)

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Application publication date: 20220524