CN114503980A - Method and device for prolonging vase life of cut vase - Google Patents

Method and device for prolonging vase life of cut vase Download PDF

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CN114503980A
CN114503980A CN202210159733.4A CN202210159733A CN114503980A CN 114503980 A CN114503980 A CN 114503980A CN 202210159733 A CN202210159733 A CN 202210159733A CN 114503980 A CN114503980 A CN 114503980A
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vase
cut
flower
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branch
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刘俊祥
古琳
马梦宇
胡耀芳
孙振元
彭辉
周红敏
张雷
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Research Institute of Forestry of Chinese Academy of Forestry
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Research Institute of Forestry of Chinese Academy of Forestry
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
    • A01N3/02—Keeping cut flowers fresh chemically
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The invention discloses a method and a device for prolonging the vase life of a cut flower. The device is a bottle-inserting container, a light source for photosynthesis of cut flower branches is arranged in the bottle-inserting container, and the light source has the luminous intensity of 100 mu mol.m‑2s‑1The above; water is stored at the bottom of the bottle inserting container. The method of the invention utilizes the characteristics of green flower branches, abundant cortex tissue chloroplasts and feasible cortex photosynthesis to obviously improve the water balance of the cut flowers, improve the opening quality of the cut flowers and prolong the vase life.

Description

Method and device for prolonging vase life of cut vase
Technical Field
The invention belongs to the field of cut flower manufacturing, and relates to a method for prolonging the cut flower vase life and a device capable of prolonging the cut flower vase life.
Background
The cut flower is cut from living plant, has ornamental value, and can be used for making plant materials such as stem, leaf, flower, fruit, etc. for flower decoration.
The vase life refers to that the cut flowers are observed every day after the vase is inserted, the sign that the ornamental value of the cut flowers is lost is that the petals are completely wilted or the bent neck is more than 90 degrees, and the total days from the beginning of the vase insertion to the day before the ornamental value is lost is recorded as the vase life of the cut flowers.
China rose is rich in flower color, fragrant in smell and high in ornamental value, is the head of the world 'four big cut flowers', but the short vase service life limits the industrial development of China rose. In order to improve the quality of cut flower vase and prolong the opening time, a large amount of research is carried out by students. For example, carbon source, stem blockage inhibitor, ethylene inhibitor, culture vegetative shoot, etc. are added into the bottle-insert liquid.
In the process of implementing the invention, the inventor finds that at least one of the following technical problems exists in the prior art:
1. the capital investment is large;
2. some components of the bottle insert (such as the ethylene inhibitor silver thiosulfate) have the risk of environmental pollution; in addition, the components of the bottle insert liquid have dosage effect, the concentration control is not good, negative effect can be brought, and the effect of prolonging the service life of the bottle insert is unstable;
3. the investment time for culturing the vegetative shoots at the early stage is long, the cost is high, the large-scale production of cut flowers is not facilitated, and the main purpose of the method is not to prolong the vase life of the cut flowers.
Disclosure of Invention
In view of the above, the invention aims to provide a cut flower preservation method which is low in cost and ecological and environment-friendly.
Another object of the present invention is to provide a device for facilitating the freshness preservation of cut flowers.
The invention provides a method for prolonging the cut flower vase life through long-term exploration and trial, multiple experiments and efforts and continuous innovation, and aims to solve the technical problems.
According to one embodiment of the method for prolonging the vase life of the cut flower, the cut flower is green in flowering branch, rich in cortex tissues and chloroplasts and capable of photosynthesis of cortex.
According to one embodiment of the method for prolonging the cut flower vase life, the length of the flower branch of the fresh cut flower is 60-65 cm, and the length of the cut flower branch subjected to photosynthesis is 1/3-2/3 of the total length of the cut flower branch.
According to one embodiment of the method for prolonging the vase life of the cut vase, the rehydration duration is 1-3 hours, and the air plug is cut off to block branch sections of the cut xylem conduit in the rehydration process.
According to a preferred embodiment of the method for prolonging the vase life of the cut flower, the length of the rehydration treatment is 2 hours.
According to a preferable embodiment of the method for prolonging the vase life of the cut vase, the branch section with the length of 10-25 cm at the lower part of the branch is cut off in the rehydration process.
According to one embodiment of the method for prolonging the cut flower vase life, water is stored in the vase container, and the lower part of a fresh cut flower branch is immersed into the water by 10-30 cm.
According to a preferred embodiment of the method for prolonging the vase life of the cut flower, the lower part of the fresh cut flower branch is immersed in water 15-20 cm.
According to one embodiment of the method for prolonging the vase life of a cut vase, the internal light intensity of the vase container is above the photosynthetic light saturation point of the cortex of the branches, and the cortex of the branches fixes CO through photosynthesis2The amount of the compound is not less than that of the generation of CO by the respiration of branches2The amount of (c).
According to one embodiment of the method for prolonging the service life of the cut vase, the light intensity below and/or above the water surface in the vase container is 100 [ mu ] mol-2s-1～300μmol·m-2s-1。
According to a preferred embodiment of the method for extending the life of the cut vase, the light intensity under and/or above the water surface inside the vase container is 200 μmol-2s-1～300μmol·m-2s-1。
According to one embodiment of the method for prolonging the vase life of a cut flower, the photosynthesis photoperiod is 12h light/12 h dark.
According to one embodiment of the method for extending the cut flower vase life of the present invention, the flower plant is a rose.
According to one embodiment of the method for prolonging the vase life of the cut flower, 1-4 pieces of compound leaves at the top end of the cut flower are reserved.
According to a preferred embodiment of the method for prolonging the vase life of the cut flower, 1-2 pieces of compound leaves at the top end of the cut flower are reserved.
The invention also provides a device for prolonging the cut flower vase life based on the method, the device is a vase container, a light source for photosynthesis of cut flower branches is arranged in the vase container, and the light source has the luminous intensity of 100 mu mol.m-2s-1The above; the bottle insert container is stored with water, and the light source is located below or/and above the water surface.
According to a preferred embodiment of the device for extending the vase life of cut flowers according to the invention, the light source is located below the water surface.
According to a preferred embodiment of the device for extending the vase life of cut flowers according to the invention, the light source is located below and above the water surface.
According to a preferred embodiment of the apparatus for extending the vase life of a cut flower according to the present invention, the light source has a luminous intensity of 200. mu. mol. m-2s-1The above.
According to a preferred embodiment of the device for prolonging the vase life of a cut flower, the light source is an LED sleeve or an LED strip or an LED post or an LED ring and/or an LED sheet.
According to a preferred embodiment of the device for prolonging the vase life of the cut vase, the height of the water stored in the vase container is 15-30 cm.
Compared with the prior art, one of the technical solutions has the following advantages:
a) the method of the invention utilizes the characteristics of green flower branches, abundant cortex tissue chloroplasts and feasible cortex photosynthesis to obviously improve the water balance of the cut flowers, improve the opening quality of the cut flowers and prolong the vase life.
b) The method can obviously improve the water balance value, the flower branch water potential and the net photosynthetic rate of leaves of the cut flowers, and the higher the light intensity is, the larger the amplification is, so that the flower diameter increase rate is obviously improved, and the fresh weight decrease rate is slowed down. By utilizing the branch cortex photosynthesis, the method can play an important role in prolonging the vase life of the cut flower and improving the opening quality of the cut flower by improving the supply of water and sugar.
c) In one embodiment of the invention, the branch section of the conduit blocking the cut xylem is cut off by the air plug in the rehydration process, so that the water supply during the vase of the cut flower is ensured, the opening quality of the cut flower is improved, and the vase service life is prolonged.
d) In one embodiment of the invention, the photosynthetic products can be fixed through cortical photosynthesis, and the photosynthetic products can be accumulated in branch cortical tissues, so that the osmotic potential of cortical cells is reduced, and the water absorption of bark is promoted. The main problem of limiting the service life of the cut flower vase in the bottle-cutting process is water stress, and illumination of branches immersed in water can promote the bark to absorb water, so that the water supply is increased, and the service life of the bottle vase is prolonged.
e) Tests prove that the vase life of the cut rose flowers is prolonged by 3d and 4d respectively compared with a control under low-light and high-light treatment.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic cross-sectional view of an apparatus for extending the vase life of a cut flower according to a preferred embodiment of the present invention.
The labels in the figure are respectively: 100 vials, 200 light sources, 300 water.
FIG. 2 shows the change of chlorophyll fluorescence parameters of different tissues of Chinese rose branches. In fig. 2: a, initial fluorescence; b, maximum fluorescence; c, maximum photochemical efficiency.
FIG. 3 is a photosynthetic-photoresponse curve of the shoot cortex of a cut rose flower.
FIG. 4 is a graph showing the influence of cortical photosynthesis on the vase life of a cut rose.
FIG. 5 is a graph showing the trend of cortical photosynthesis affecting the fresh weight and the rate of change in flower diameter of cut rose flowers.
FIG. 6 is a graph showing the trend of the effect of cortical photosynthesis on leaf gas exchange of cut rose flowers. In fig. 6, a, net photosynthetic rate; b, transpiration rate; c, porosity conductivity; d, intercellular carbon dioxide concentration.
FIG. 7 is a graph showing the influence of cortical photosynthesis on the water potential of cut stems of Chinese rose.
Detailed Description
The following description is made with reference to the accompanying drawings and a specific embodiment.
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it may not be further defined and explained in subsequent figures.
See fig. 1. The present embodiment first describes an apparatus for extending the life of a cut flower,the device is a bottle-inserting container 100, a light source 200 for photosynthesis of cut flower branches is arranged in the bottle-inserting container, and the light source has the luminous intensity of 100 mu mol.m-2s-1The above; the vial insert container has water 300 stored therein. The light source 200 provides 100 μmol. m below and above the water surface in the vial insertion container 100-2s-1The above light intensity provides especially 200 μmol · m-2s-1The above light intensity. The light source is an LED sleeve or an LED lamp strip or an LED lamp post or an LED lamp ring or an LED lamp piece, and the combination thereof.
The method for prolonging the vase life of a cut vase described in the embodiment specifically comprises the following steps: taking fresh cut flowers, carrying out rehydration treatment for 1-3 days, cutting branches with the length of 15cm at the lower parts during rehydration treatment, plugging air plugs at the lower parts of the branches to block a guide pipe of a cut xylem, ensuring water supply during vase of the cut flowers after cutting, and inserting the cut flowers into a vase container capable of ensuring photosynthesis of cut branch cortex after rehydration treatment. The cut flowers are green in flower branches, rich in cortex tissues and chloroplasts and feasible for photosynthesis of cortex. In this example, rose was selected as the cut flower, and the light saturation point was 200. mu. mol. m-2s-1. The length of the flower branch of the fresh cut flower is 60-65 cm, and the length of the cut flower branch for photosynthesis is 1/2-2/3 of the total length of the cut flower branch. The top 2 compound leaves of the cut flower branch are reserved. The light intensity in the bottle-inserted container was 100. mu. mol. m-2s-1～300μmol·m-2s-1Preferably, the light intensity of the photosynthetic saturation point of the cortex of the branch is selected to ensure that the cortex of the branch fixes CO through photosynthesis2The amount of the compound is not less than that of the generation of CO by the respiration of branches2The amount of (c). The bottle container is stored with water, and the lower part of the fresh cut flower branch is immersed in the water. Photosynthetic products can be fixed through cortical photosynthesis, and the photosynthetic products can be accumulated in branch cortical tissues, so that the osmotic potential of cortical cells is reduced, and bark water absorption is promoted. The main problem of limiting the service life of the cut flower vase in the bottle-cutting process is water stress, and illumination of branches immersed in water can promote the bark to absorb water, so that the water supply is increased, and the service life of the bottle vase is prolonged.
To further illustrate the technical effects of the present invention, the following experiments are conducted.
In the test, fresh cut flowers of Chinese rose 'legend' (Rosa hybrida cv. 'Legends') are selected, and the length of flower branches is 60-65 cm. Pruning the length of the flowering branch in water to 40cm, and only keeping 2 compound leaves at the top. After rehydration for 2h, different light treatments were performed.
In the test, the cut flowers after rehydration are taken, and the photosynthetic pigment concentration of the cortex and the leaves of the branches, the chlorophyll fluorescence parameter of the cross section of the branches and the cortex photosynthetic-photoresponse curve are measured and repeated for 3 times.
Inserting the cut flower after rehydration into a transparent glass test tube filled with 100ml of deionized water, and placing the test tube in an illumination incubator with layered light control at the upper part and the lower part. The part above the water surface (flower, leaf, flower branch) is on the upper layer, and the part below the water surface (flower branch immersed in water) is on the lower layer. The surface of the test tube below the water surface is wrapped by aluminum foil paper (light transmittance is 0%) and a preservative film (light transmittance is 40%), and the wrapped test tube is respectively used as a Control (CK) and a low light treatment (low light, LL), and the unwrapped test tube is used as a high light treatment (HL). The test tube is sealed with a preservative film to avoid water evaporation. Setting the illumination intensity of the upper layer of the incubator to be 20 mu mol.m-2·s-1Adjusting the illumination intensity of the lower layer to ensure that the Photosynthetically Active Radiation (PAR) of the surfaces of the CK, LL and HL treated flower branches near light sources is 0 mu mol.m-2·s-1、80μmol·m-2·s-1And 200. mu. mol. m-2·s-1. Each treatment was arranged with 6 cut flowers for in situ observation and 12 cut flowers for water potential measurement sampling, for a total of 54 cut flowers. The humidity of the incubator is set to be 60 percent, the temperature is 25 ℃, and the photoperiod is 12h of illumination/12 h of darkness. During the treatment period, water is supplemented every day to ensure that the volume of the bottle insert liquid is unchanged.
1. Determination of photosynthetic property of Chinese rose branch cortex
(1) Determination of photosynthetic pigment content in branch cortex and leaf
And (3) taking 1g of cortex in the middle of the branch and 1g of functional leaves respectively, grinding and extracting, and then calculating the photosynthetic pigment content of each sample according to the constant volume and the fresh weight of the sample.
(2) Measurement of chlorophyll fluorescence parameter of cross section of branch
The cross section of the middle part of the branch was taken, placed in water to dark adapt for 30min, and the maximum photochemical efficiency of the cross section was measured using a Fluorescence kinetic microscope (Photon Systems Instruments, Czech Republic), and the radial change in the maximum photochemical efficiency was analyzed using the FluorCam software.
(3) Determination of cortical photo-response curves
The net photosynthetic rate (Pn) of the branches of the rose at different light intensities was measured using an LI-6400XT photosynthesizer (Li-cor Inc., Lincoln, NE, USA) and the photoresponse curve was plotted.
2. Determination of bottle insertion index
(1) Life of bottle insert
Observing the cut flowers every day after treatment, taking the fact that all petals are wilted or the bent neck is more than 90 degrees as the sign of the loss of the ornamental value of the cut flowers, and recording the total days from the beginning of the bottle insertion to the day before the loss of the ornamental value as the bottle insertion life of the cut flowers.
(2) Fresh weight and flower diameter change rate
The initial fresh weight (Wo) and initial diameter (D) of the cut flowers were recordedO) Then, the fresh weight (W) of the cut flowers was counted every dayi) Maximum diameter of flower (D)i) The fresh weight change rate (Wc) and the cut flower diameter change rate (Dc) were calculated according to the notations 1 and 2.
Wc(％)＝(Wi-Wo). times.100/Wo formula 1.
Dc(％)＝(Di-DO)×100/DOEquation 2.
(3) Water balance value
The water absorption capacity of the branches and the water loss of the branches of the flowers are counted every day, and the water absorption capacity of the branches is the difference between the weight of the solution and the weight of the bottle in two adjacent days (W)a) The water loss of the flowering branch is the difference (W) between the total weight of the solution, the bottle weight and the flowering branch on two adjacent daysl). Calculate the moisture balance value (W) from Notification 3b)。
Wb(g)＝Wa-WlEquation 3.
(4) Blade gas exchange parameters
Gas exchange parameters including Pn, stomatal conductance (Cond), intercellular carbon dioxide concentration (Ci) and transpiration rate (Tr) were determined for each treated leaf using LI-6400XT photosynthesizer (LI-cor inc., Lincoln, NE, USA) every 2 d.
3. Measurement of flower Branch Water potential
3 cut flowers were selected from each treatment every 2d, and the cut flowers were placed upside down in a pressure chamber (Model1505D, PMS instruments co., Albany, OR, USA) under pressure, and the pressure was stopped until the cut was drained of water, and the water potential of the flower branches was recorded.
Statistical analysis
Analysis of variance and comparison of significance of differences were performed using SPSS19.0 software with significance level P <0.05, summary data and charting with Excel 2007.
Cortex photosynthetic property of Chinese rose branches
a. Composition of photosynthetic pigment in branch cortex
As shown in Table 1, the contents of chlorophyll a, chlorophyll b, carotenoid and total chlorophyll in the leaves of Chinese rose are all obviously higher than those in the cortex of branches (P < 0.05). Wherein the cortex total chlorophyll content is 0.68mg.g-1FW, about 24.3% of leaf, 0.1mg.g carotenoid content-1FW, about 28.6% of the blade. The ratio of chlorophyll a to chlorophyll b in the cortex is lower than in leaves, while the ratio of carotenoids to total chlorophyll is slightly higher than in leaves.
TABLE 1 compositional differences in photosynthetic pigments in shoot cortex and leaves
Figure BDA0003513978560000061
Note: values are mean ± sem of 3 replicates. Different letters in the same column indicate that different treatments differed significantly at the p <0.05 level.
b. Photochemical characteristics of shoot cortex chloroplast
The rose shoots have chlorophyll fluorescence from the epidermis to the medulla (fig. 2A), indicating that there is a distribution of chloroplasts in living cells of different tissues. After the saturation pulse was turned on, the fluorescence intensity of each site was significantly increased (FIG. 2B), indicating that chloroplasts of each site had photochemical activity. The maximum photochemical efficiency exhibited a decreasing radial progression from epidermis to medulla, with the highest photochemical efficiency of the cortical tissue chloroplasts (fig. 2C).
c. Photosynthetic photoresponse characteristic of shoot cortex
The respiratory rate of the branches is 0.84 mu molCO2·m-2s-1Photosynthetically active radiation of 50 [ mu ] mol m-2s-1When the net photosynthetic rate is 0. mu. molCO2·m-2s-1At the moment, the cortex of the branch can photosynthesize CO released by the respiration of the branch2All are recycled. Subsequently, the net photosynthetic rate shows a tendency to increase first and then decrease. At a light intensity of 200. mu. mol. m-2s-1When the net photosynthetic rate of the branches reaches the peak, the net photosynthetic rate of the branches is 0.15 mu molCO2·m-2s-1(FIG. 3).
c. Influence of cortex photosynthesis on vase life of Chinese rose cut flowers
As shown in fig. 4, the vial insertion lives for HL, LL, and CK treatments were 11.67d, 10.67d, and 7.67d, respectively. Wherein, the vase life of the bottle subjected to HL treatment is obviously increased by 4d compared with CK, and is obviously increased by 1d (P is less than 0.05) compared with LL treatment, which indicates that the vase life of the Chinese rose cut bottle is in direct proportion to the illumination intensity of the stem.
The quality of the cut flowers treated by illumination is obviously superior to that of the cut flowers treated by darkness in the same vase time. When the bottle is inserted to the 8 th day, in the aspect of flower quality, the cut flower petals treated by CK have serious water loss wilting, the flower petals fall off and the flower branches bend the neck; LL treated cut flower, the flower core is gradually exposed, and the outermost layer of petals loses water and wilts; the cut flower petals treated by HL still have no wilting phenomenon. In terms of leaf quality, LL treated leaves sagged and lose water slightly compared to HL treatment; CK treatment caused severe water loss and wilting, with obvious green loss.
d. Influence of cortex photosynthesis on fresh weight and flower diameter change rate of Chinese rose cut flowers
As shown in fig. 5A, the cut flower fresh weight drop rate generally shows a tendency of CK > LL > HL. At 8d, the fresh weight of CK treated cut flowers was reduced by 28.63% from the initial fresh weight, significantly higher than 22.65% for LL treatment and 20.52% for HL treatment (P < 0.05). The difference between HL treatment and LL treatment reaches a remarkable level (P is less than 0.05) on the bottle insertion 9 th and 10d, and the effect of slowing down the fresh weight reduction of cut flowers by the HL treatment is more obvious.
As shown in fig. 5B, the flower diameter is in the trend of ascending first and then descending, and the CK, LL and HL treatments reach the maximum flower diameter at 5d, 8d and 9d respectively, and are increased by 27.64%, 37.8% and 43.26% respectively compared with the initial flower diameter. After treatment 1d, the rate of change of flower diameter was significantly higher for both HL and LL treatments than for CK (P < 0.05). When the treatment time was extended to 9d and 10d, the difference between HL and LL treatment reached a significant level (P < 0.05). The result shows that the rate of increasing the flower diameter can be obviously improved by the photosynthesis of the cortex of the branch, and the time of continuously increasing the flower diameter is prolonged.
e. Influence of cortex photosynthesis on water balance of Chinese rose cut flowers
As shown in Table 2, the water uptake by flowering branches was always HL > LL > CK, and the difference between HL and CK treatments increased with the number of days. The water uptake for the cannulation 2d, HL treatment was significantly higher than for LL and CK treatments (P < 0.05); differences between vial insertion 4d, 3 treatments all reached significant levels (P < 0.05). The results of flower branch water loss and water absorption are consistent, and HL > LL > CK is also shown.
From the results of the water balance values, all 3 treatments showed a tendency to decrease and then increase, but only the HL treatment remained positive at vial insertion 1d, i.e. water uptake > water loss. The vial insertion 8d, HL and LL treatments were significantly higher than the CK treatment, and the difference between the vial insertion 10d, HL and LL treatments reached a significant level (P < 0.05). The results show that cortex photosynthesis can delay the reduction of the water balance value of the cut flowers, and the effect is more obvious in the later stage of bottle insertion.
TABLE 2 influence of cortical photosynthesis on water uptake, water loss and water balance values of cut rose flowers
Figure BDA0003513978560000081
Note: values are mean ± sem of 6 replicates. Different letters in the same column indicate that different treatments differed significantly at the p <0.05 level.
f. Influence of cortex photosynthesis on leaf gas exchange of Chinese rose cut flowers
As shown in FIG. 6A, Pn for CK treatment decreased continuously 2d before vial insertion, while Pn for HL and LL treatment increased. Pn was significantly higher for HL treatment than for LL and CK treatment 6d prior to vial insertion (P < 0.05). The difference between the vial insertion 8d, LL and CK treatments reached a significant level (P < 0.05). The net photosynthetic rate of the leaves is in direct proportion to the intensity of light received by the stems, which shows that the photosynthesis of the leaves has a dependence on the photosynthesis of the stem cortex.
Tr (FIG. 6B) and Cond (FIG. 6C) have the same tendency as Pn, and show a tendency of HL > LL > CK as a whole. Combining the results of Pn (FIG. 6A), Cond (FIG. 6C) and Ci (FIG. 6D) of leaves, the decrease of Pn by HL treatment was accompanied by the decrease of Cond and Ci after 2D of bottle insertion, indicating that the stomata restriction factor dominates the decrease of photosynthetic rate; for LL and CK treatments, Pn continued to decrease, but Ci tended to increase at 4d and 6d, respectively, indicating that the decrease in photosynthetic rates of LL treatment with 4d phial and CK treatment with 6d phial was mainly due to non-stomatal restriction factors, and may be associated with the inhibition of photosynthetic cell viability.
g. Influence of cortex photosynthesis on water potential of cut stems of Chinese rose
As shown in FIG. 7, the water potential of the flower stems of different treatments all showed a descending trend, and the descending speed is shown as CK > LL > HL. Except for 6d, the stem water potential for HL treatment was significantly higher than for LL and CK treatments. Phialides 6d and 8d, LL treated stem water potential was significantly higher than CK treatment (P < 0.05). This shows that the photosynthetic capacity of the branch cortex can obviously relieve the decrease of the water potential of the flower stem, and the relieving effect is in direct proportion to the light intensity.
Chlorophyll synthesis requires the participation of light. The results of this example show that chlorophyll concentration in the cortex of the rose shoots is significantly lower than in leaves, which may be associated with lower chlorophyll synthase expression and weaker light intensity in the cortex tissue. Cortical photosynthesis relies on the absorption and transformation of limited light energy in the shoot by the cortical chloroplasts. The higher chlorophyll b/chlorophyll a ratio of rose cortex tissue compared to leaves facilitates the capture of the limited light energy transmitted through the bark by the cortical chloroplasts. The maximum photochemical efficiency of different tissues on the cross section of the Chinese rose branches shows a trend of remarkably reducing from cortex to pith, and the maximum photochemical efficiency of the cortex is remarkably higher than that of xylem and pith. Photosynthetic CO of woody plant cortex2The re-fixing rate is about 73 percent, and the Chinese rose branches are 50 mu mol.m-2s-1Can release CO from branches by breathing under the light intensity of2All are recycled. At 200. mu. mol. m- 2s-1Can even exhibit a positive net photosynthetic rate (0.15 μmolCO) at saturated light intensity of the rose branches2·m-2s-1) This shows that the Chinese rose branches can not only perform the function of carbon recovery, but also absorb and utilize external CO2。
The water shortage is an important factor influencing the quality of cut flowers and limiting the vase life. For the cut flower, the water absorption efficiency is greatly reduced because the cut is easily blocked by secretion and bacteria during the bottle inserting process. Therefore, it is certainly important to maintain the moisture supply of the cut flowers if the water absorption of the bark can be improved and other moisture sources except for the cut are added. The experiment result of the embodiment shows that the photosynthesis of the cortex of the branch can remarkably promote the absorption of the cut flower on water and maintain the water balance of the cut flower. Therefore, the prolonging of the vase life of the cut rose flowers by branch cortex photosynthesis is probably related to the cortex photosynthesis which promotes bark water absorption and repairs xylem embolism in flower stems and ensures the smoothness of water delivery of a catheter.
Sugar plays an important role in cut flower opening, and accumulation of sugar in petals, reduction of osmotic potential and subsequent inflow of water are considered to be main causes of increase in flower diameter and flower opening of Chinese rose cut flowers. The sugar in the petals is partly derived from non-structural carbohydrates stored in the petals and partly depends on the delivery of leaf carbohydrates. Leaf photosynthesis depends on gas exchange between stomata and the outside, the opening of the stomata is strictly controlled by the water potential of the leaves, and the stomata of the leaves are closed in advance due to the too fast reduction of the water potential, so that the plants are in danger of carbon starvation. The increase in the rate of increase in floral diameter by cortical photosynthesis and the increase in duration of increase in floral diameter may be associated with an increase in leaf photosynthetic rate.
The vase life is always the focus of the attention of the preservation of the picked fresh cut flowers, and the important function of cortex photosynthesis in woody plants is highly emphasized in recent years. According to the invention, the photosynthetic property of the cortex of the branch is applied to the postharvest preservation of the cut rose flower, and research results show that the photosynthetic property of the cortex of the branch can obviously improve the water and sugar supply of the cut rose flower, prolong the vase life of the cut rose flower, improve the opening quality of the cut rose flower and provide a theoretical basis for the application of the cortex photosynthetic property in the postharvest preservation of the fresh cut rose flower.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.

Claims (10)

1. A method for prolonging the cut flower vase life is characterized in that fresh cut flowers are taken, subjected to rehydration treatment and then inserted into a vase container capable of ensuring photosynthesis of cut flower branch cortex.
2. The method for extending the vase life of a cut flower as claimed in claim 1, wherein said cut flower is a flower with green flower branches, rich chloroplast of cortex tissue, and capacity to photosynthesize cortex.
3. The method for prolonging the vase life of a cut flower according to claim 1, wherein the length of the flower branch of the fresh cut flower is 60-65 cm, and the length of the branch of the cut flower subjected to photosynthesis is 1/3-2/3 of the total length of the branch of the cut flower.
4. The method for prolonging the vase life of the cut vase according to claim 1, wherein the rehydration duration is 1-3 hours, and the branch section of the conduit of the cut xylem is blocked by cutting off the air plug during the rehydration process.
5. The method for prolonging the vase life of a cut flower according to claim 1, wherein the vase container stores water, and the lower part of a fresh cut flower branch is immersed in the water by 10-30 cm.
6. The process of claim 5The method for prolonging the vase life of a cut vase is characterized in that the internal light intensity of the vase container is above the photosynthetic light saturation point of the cortex of the branch, and the cortex of the branch fixes CO through photosynthesis2The amount of the compound is not less than that of the generation of CO by the respiration of branches2The amount of (c).
7. The method for extending the life of a cut flower vase according to claim 5, wherein the intensity of light below and/or above the water surface inside the vase container is 100 μmol-m-2s-1～300μmol·m-2s-1(ii) a The photosynthesis photoperiod is 12h of light/12 h of dark.
8. The method for extending the vase life of a cut flower according to claim 2, wherein the flower plant is a rose.
9. The method for prolonging the vase life of a cut flower according to claim 1 or 8, wherein the cut flower has 1 to 4 pieces of compound leaves at the top.
10. An apparatus for prolonging the life of cut flower vase based on the method according to any one of claims 1 to 9, wherein the apparatus is a vase container, a light source for photosynthesis of cut flower branches is arranged in the vase container, and the light source has a luminous intensity of 100 μmol-m-2s-1The above; the bottle insert container is stored with water, and the light source is located below or/and above the water surface.
CN202210159733.4A 2022-02-22 2022-02-22 Method and device for prolonging vase life of cut vase Pending CN114503980A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170000112A1 (en) * 2013-12-24 2017-01-05 Enhold B.V. Method of preserving cut roses during transportation and storage and shipment kit containing cut roses
US20170172085A1 (en) * 2014-01-27 2017-06-22 Knud Jepsen A/S Bouquet of cut flowers
CN107374204A (en) * 2017-08-01 2017-11-24 华南理工大学 A kind of vase of high-preserved effect
CN108552166A (en) * 2018-06-19 2018-09-21 商丘师范学院 A method of extending Chinese rose Vase time

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170000112A1 (en) * 2013-12-24 2017-01-05 Enhold B.V. Method of preserving cut roses during transportation and storage and shipment kit containing cut roses
US20170172085A1 (en) * 2014-01-27 2017-06-22 Knud Jepsen A/S Bouquet of cut flowers
CN107374204A (en) * 2017-08-01 2017-11-24 华南理工大学 A kind of vase of high-preserved effect
CN108552166A (en) * 2018-06-19 2018-09-21 商丘师范学院 A method of extending Chinese rose Vase time

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