CN114403905B - Rodent experimental animal vision electrophysiology detection placement platform - Google Patents
Rodent experimental animal vision electrophysiology detection placement platform Download PDFInfo
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- CN114403905B CN114403905B CN202111662804.4A CN202111662804A CN114403905B CN 114403905 B CN114403905 B CN 114403905B CN 202111662804 A CN202111662804 A CN 202111662804A CN 114403905 B CN114403905 B CN 114403905B
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- 238000001514 detection method Methods 0.000 title claims abstract description 27
- 241000283984 Rodentia Species 0.000 title claims abstract description 26
- 230000007831 electrophysiology Effects 0.000 title claims abstract description 21
- 238000002001 electrophysiology Methods 0.000 title claims abstract description 21
- 238000010171 animal model Methods 0.000 title claims abstract description 15
- 230000004438 eyesight Effects 0.000 title claims abstract description 14
- 230000007704 transition Effects 0.000 claims abstract description 45
- 238000002474 experimental method Methods 0.000 claims abstract description 23
- 230000000638 stimulation Effects 0.000 claims abstract description 8
- 230000000007 visual effect Effects 0.000 claims description 16
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 10
- 241000699670 Mus sp. Species 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 7
- 230000004936 stimulating effect Effects 0.000 claims description 7
- 238000004070 electrodeposition Methods 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 abstract description 19
- 230000000694 effects Effects 0.000 abstract description 9
- 230000008859 change Effects 0.000 abstract description 3
- 238000013480 data collection Methods 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 210000003128 head Anatomy 0.000 description 3
- 230000004382 visual function Effects 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 210000004087 cornea Anatomy 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 230000000763 evoking effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 210000001525 retina Anatomy 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000700198 Cavia Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000869 occipital lobe Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000000857 visual cortex Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
- A61B5/316—Modalities, i.e. specific diagnostic methods
- A61B5/398—Electrooculography [EOG], e.g. detecting nystagmus; Electroretinography [ERG]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
- A61B5/25—Bioelectric electrodes therefor
- A61B5/279—Bioelectric electrodes therefor specially adapted for particular uses
- A61B5/297—Bioelectric electrodes therefor specially adapted for particular uses for electrooculography [EOG]: for electroretinography [ERG]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
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Abstract
The invention discloses a rodent experimental animal vision electrophysiology detection placement platform, which is characterized by comprising the following components: a carrier plate, an electrode; the electrode is dynamically connected to the bearing flat plate through a connecting block; the electrode tip is provided with a stimulation component, and a transition cylinder in threaded connection with the bearing flat plate is arranged in the middle; the transition cylinder is in threaded connection with the connecting block, and a locker which is used for unlocking the locking state of the transition cylinder and the bearing flat plate or the transition cylinder through pressing is arranged at the bottom of the connecting block; the device has designed a brand-new animal experiment platform of form stable on oneself animal loading platform's basis, passes through screw-thread fit with the electrode, and dynamic stability sets up on loading plate, and corresponds the three positions of the mouse that treats the stimulus, compares in traditional self-control platform, and the during operation is more stable, is difficult for taking place dynamic change, causes experimental data collection error, influences experimental effect, helps the promotion of experimental quality, also can reduce unnecessary trouble in the experiment simultaneously.
Description
Technical Field
The invention belongs to the field of animal experiment instruments, and particularly relates to a rodent experiment animal vision electrophysiology detection placement platform.
Background
Visual electrophysiological examination belongs to a non-invasive objective visual function examination method by recording visual system bioelectric activities to diagnose diseases from visual function angles, identify curative effects and judge prognosis, and mainly comprises Electroretinogram (ERG) and visual evoked potential (Visual evoked potential, VEP).
Animal vision electrophysiological detection is an experimental technique that uses an electrophysiological instrument to measure bioelectrical activity produced in the visual system when the retina of an animal is irradiated with light or stimulated by an image, so as to evaluate the visual function thereof. The main detection process is as follows: recording electrodes are placed on the surface of eyeball or occipital lobe area of brain to record the electric signals generated by exciting retina and visual cortex by optical stimulus.
Rodent laboratory animals including rats, mice and guinea pigs can be subjected to corresponding visual electrophysiological detection. In the existing animal vision electrophysiological detection technology, a rodent experimental animal is usually placed on a common platform, and self-made electrodes are placed at the positions of corresponding electrodes of the animal (for example, when ERG detection is carried out, a recording electrode is placed at the center of the cornea of the detected eye of the animal, a reference electrode is placed at one cheek part, a grounding electrode is inserted under the tail skin, a recording electrode is placed under the middle point of the connecting line of two ears of the animal when VEP is carried out, the reference electrode is placed at one cheek part, and the grounding electrode is inserted under the tail skin). However, since rodent experimental species are different, the body sizes are greatly different, and individual body sizes are different among the rodent experimental animals of the same species, the actual operation needs to adjust the electrode position according to the animal size and body type. In addition, because the head of the rodent experimental animal is approximately triangular, the cornea edge plane is not perpendicular to the ground, the operation of adjusting the electrode is relatively inconvenient, the moving position is difficult to continuously adjust at times, the working procedure is complicated, the electrode is easy to be unstable to fix or fall off, the electrode state is unstable, the acquisition of visual electrophysiological signals is influenced, the accurate acquisition of experimental data is influenced, and unnecessary troubles are caused.
Disclosure of Invention
In order to solve the technical problems, the invention provides a rodent experimental animal vision electrophysiology detection placement platform, which solves the problems of inconvenience of a self-made experimental platform and unstable working state part of the self-made platform in the traditional experiment.
In order to achieve the technical effect of solving the technical problems, the invention is realized by the following technical schemes: a rodent experimental animal vision electrophysiology detection placement platform, comprising: a carrier plate, an electrode;
the electrode is dynamically connected to the bearing flat plate through a connecting block;
the electrode tip is provided with a stimulation component, and a transition cylinder in threaded connection with the bearing flat plate is arranged in the middle; the transition cylinder is in threaded connection with the connecting block, and a locker which is used for unlocking the locking state of the transition cylinder and the bearing flat plate or the transition cylinder through pressing is arranged at the bottom of the connecting block;
further, the upper position, the left (right) position and the lower position of the upper surface of the bearing flat plate are provided with T-shaped groove bodies which are in sliding connection with a connecting block, two sides of the bearing flat plate are respectively fixedly provided with a binding belt and a magic tape for sticking and fixing the binding belt, the upper part of the connecting block penetrates through threaded holes of a front-back threaded connection transition cylinder of the connecting block, the lower part of the connecting block is a T-shaped component which is in sliding connection with the T-shaped groove body, the locking device is dynamically arranged in the connecting block, and the pressing end of the locking device penetrates to one side of the bottom of the connecting block;
further, the locking device comprises a side clamping member connected with the pressing end and a thread clamping member dynamically driven to stretch out and draw back by the side clamping member, wherein the side clamping member is arranged in a C-shaped groove in the connecting block, an arc-shaped clamping connector is telescopically clamped into a groove in the side wall of the groove body, the thread clamping member is arranged in a cylindrical groove on one side of the C-shaped groove in the connecting block, the top concave end of the thread clamping member is clamped with a transition cylinder thread, the side clamping member is provided with a driving end matched with the transition end of the thread clamping member in an inclined plane, and the driving end of the side clamping member is compressed inwards and is matched with the transition end to drive the thread clamping member to move downwards;
further, a reset spring for providing a clamping state holding moment is arranged between the side clamping component and the groove wall of the C-shaped groove and between the thread clamping component and the cylindrical groove;
further, the electrode also comprises a lead movably connected to the inner cavity of the transition cylinder, and the stimulation component is movably connected with the transition cylinder through a clamping groove; the electrode is divided into a reference electrode connected to the upper part of the plane of the bearing platform, a recording electrode on the side edge and a grounding electrode on the lower part, the stimulating members of the reference electrode and the recording electrode are electrode clamps which can be opened movably, and the stimulating members of the grounding electrode are sharp electrode needles;
further, the transition cylinder of the recording electrode is in transition connection with an electrode clip through a mechanical joint arm with a joint belt damping of a three-joint arm, and the electrode clip is movably connected to the end head of the mechanical joint arm; the mechanical knuckle arm joint is a universal joint;
further, the electrode clamp comprises an upper clamping piece and a lower clamping piece, the upper clamping piece is hinged with the lower clamping piece at the middle part, and a reset spring is arranged between the lower clamping piece and the electrode clamp main body;
further, the lower surface of the upper clamping piece is attached to the upper surface of the lower clamping piece, electrode plates are arranged on the lower clamping piece, and the lead is communicated with the electrode plates on the upper clamping piece and the lower clamping piece;
still another object of the present invention is to provide a method for using a rodent experimental animal vision electrophysiology detection placement platform:
1. placing the device on a plane;
2. placing the anesthetized mice on the platform;
3. pressing down the pressing end of the locker;
4. sliding the connecting block to a proper position;
5. rotating the transition cylinder 22 to adjust the coverage of the electrode until the coverage is suitable for the position of the experimental mouse, and loosening the locker to lock the whole electrode position;
6. clamping electrodes at three parts to the parts to be stimulated of the mice;
7. connecting an electrode instrument through a wire, and starting an experiment;
8. at the end of the experiment, the mice were removed.
The beneficial effects of the invention are as follows:
1. the device designs a brand new animal experiment platform with stable form on the basis of the animal bearing platform, the electrodes are dynamically and stably arranged on the bearing plate through threaded fit, and the corresponding detection of the animal to be detected is realized, compared with the traditional self-made platform, the device is more stable in working, and is not easy to generate dynamic change, so that experiment data collection errors are caused, the experiment effect is influenced, the improvement of experiment quality is facilitated, and meanwhile, unnecessary troubles in the experiment can be reduced;
2. secondly, the electrodes arranged by the device are connected through threads, the range is adjustable, the device can be suitable for animals with different body types and sizes, and the electrodes can be better contacted with or placed into corresponding parts of the animals at proper positions by adjusting the positions of the electrodes, so that the electrode effect of the experiment can be improved;
3. the device is additionally provided with a locker with a locked position on the electrode, and the position of the electrode is regulated in a dual mode of a slide rail and screw thread regulation, the slide rail is used for large-scale rapid regulation, the screw thread regulation is used for accurately regulating the small distance of the electrode, the mouse adaptation capability of the whole experiment platform is improved, and convenience is provided for an operator to regulate the position of the electrode; simultaneously, the locker can be used for simultaneously adjusting and locking the sliding rail and adjusting the threads, and the locker can be directly pressed or loosened in actual operation to directly realize the locking adjustment of two degrees of freedom of movement, so that the actual operation flow is reduced, and the adjustment of an operator to the electrode is facilitated.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of a rodent experimental animal vision electrophysiology detection placement platform;
FIG. 2 is a schematic diagram of an electrode reference electrode and its attached structure of a rodent visual electrophysiology detection placement platform;
FIG. 3 is a schematic diagram of the side structure of an electrode reference electrode of a rodent visual electrophysiology detection placement platform;
FIG. 4 is a schematic view in section of the A-A plane of FIG. 3;
FIG. 5 is an enlarged schematic view of a portion of FIG. 4 at location 3;
FIG. 6 is a schematic partial cross-sectional view of the connection block portion of a rodent experimental animal visual electrophysiology detection placement platform;
FIG. 7 is a schematic diagram of the structure of a lateral locking member of a rodent visual electrophysiology detection positioning platform;
FIG. 8 is a schematic view of the thread locking structure of a rodent experimental animal vision electrophysiology detection placement platform;
in the drawings, the list of components represented by the various numbers is as follows:
the device comprises a 1-bearing flat plate, 11-connecting blocks, 112-side locking devices, 1121-driving ends, 1122-clamping ends, 1131-concave ends, 1132-transition ends, 113-thread locking devices, 114-reset inflammatory yellow, 12-T-shaped groove bodies, 121-grooves, 122-C-shaped grooves, 123-cylindrical grooves, 13-magic tapes, 14-restraint belts, 2-reference electrodes, 21-electrode clamps, 211-upper clamping pieces, 212-lower clamping pieces, 213-reset springs, 214-electrode plates, 22-transition cylinders, 23-wires, 4-recording electrodes, 41-mechanical joint arms, 5-grounding electrodes and 51-motor needles.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Referring to fig. 1 to 5, a rodent experimental animal vision electrophysiology detection placement platform is characterized by comprising: a carrying plate 1 and an electrode;
the electrodes are dynamically connected to the bearing flat plate 1 through the connecting blocks 11;
the electrode tip is provided with a stimulating component, and a transition cylinder 22 in threaded connection with the bearing flat plate is arranged in the middle; the transition cylinder is in threaded connection with the connecting block, and a locker which is used for unlocking the locking state of the connecting block 11 and the bearing flat plate 1 or the transition cylinder 22 by pressing is arranged at the bottom of the connecting block;
the upper, left (right) and lower positions of the upper surface of the bearing flat plate 1 are provided with T-shaped groove bodies 12 which are in sliding connection with a connecting block 11, two sides of the bearing flat plate 1 are respectively and fixedly provided with a binding belt 14 and a magic tape 13 for pasting and fixing the binding belt 14, the upper part of the connecting block 11 penetrates through threaded holes of a front-rear threaded connection transition cylinder 22, the lower part of the connecting block is a T-shaped component which is in sliding connection with the T-shaped groove bodies 12, the locker is dynamically arranged in the connecting block 11, and the pressing end of the locker penetrates to one side of the bottom of the connecting block 11;
the locking device comprises a side clamping member 112 connected with the pressing end and a thread clamping member 113 which is dynamically driven to stretch by the side clamping member 112, wherein the side clamping member 112 is arranged in a C-shaped groove 122 in the connecting block 11, an arc clamping connector 1122 of the side clamping member is stretched and clamped into a groove 121 on the side wall of the groove body of the C-shaped groove 122, the thread clamping member 113 is arranged in a cylindrical groove 123 on one side of the C-shaped groove 122 in the connecting block 11, a top concave end 1131 of the thread clamping member is clamped with a transition cylinder 22 thread, the side clamping member 112 is provided with a driving end 1121 of which the inclined surface is matched with a transition end 1132 of the thread clamping member 113, the side clamping member 112 is compressed inwards, and the driving end 1121 of the side clamping member is matched with the transition end 1132 to drive the thread clamping member 113 to move downwards;
a return spring 114 for providing a holding torque in the clamping state is arranged between the side clamping member 112 and the wall of the C-shaped groove 122 and between the threaded clamping member 113 and the cylindrical groove 123;
the electrode also comprises a lead 23 movably connected to the inner cavity of the transition cylinder 22, the stimulation component is movably connected with the transition cylinder 22 through a clamping groove, and the stimulation component can rotate relative to the transition cylinder 22, so that the coverage range of the electrode is adjusted through the transition cylinder 22 under the condition that the stimulation component is not moved;
the electrode is divided into a reference electrode 2 connected to the upper part of the plane of the bearing platform, a recording electrode 4 at the side edge and a grounding electrode 5 at the lower part, the stimulating members of the reference electrode 2 and the recording electrode 4 are electrode clamps 21 which can be opened movably, the stimulating member of the grounding electrode 5 is a sharp electrode needle 51, and the electrode needle 51 is directly pricked into the tail of a mouse to carry out electrode;
the transition cylinder 22 of the recording electrode 4 is in transition connection with the electrode clamp 21 through a mechanical joint arm 41 with a joint damping of three joint arms, and the electrode clamp 21 is movably connected to the end head of the mechanical joint arm 41;
the joints of the mechanical joint arm 41 are universal joints; the angle between the animal and the electrode is convenient to adjust, so that the limbus plane is parallel to the recording electrode;
the outer surface of the middle section of the transition cylinder 22 is provided with threads which are matched with the threaded holes of the connecting block 11;
the electrode clamp 21 comprises an upper clamping piece 211 and a lower clamping piece 212, wherein the upper clamping piece 211 and the lower clamping piece 212 are hinged at the middle part, and a reset spring 213 is arranged between the lower clamping piece 212 and the electrode clamp 21 main body so as to clamp the upper clamping piece 211 and the lower clamping piece 212 through elastic force, namely clamp the mouse part;
the lower surface of the upper clamping piece 211 is attached to the upper surface of the lower clamping piece 212, and electrode plates 214 are arranged on the upper clamping piece 211 and the lower clamping piece 212, and the wires 23 are communicated with the electrode plates 214 on the upper clamping piece 211 and the lower clamping piece 212.
Example 2
The embodiment is a specific application method and steps of a rodent experimental animal vision electrophysiology detection placement platform:
1. placing the device on a plane;
2. placing the anesthetized mice on the platform;
3. pressing down the pressing end of the locker;
4. sliding the connecting block to a proper position;
5. rotating the transition cylinder 22 to adjust the coverage of the electrode until the coverage is suitable for the position of the experimental mouse, and loosening the locker to lock the whole electrode position;
6. clamping electrodes at three parts to the parts to be stimulated of the mice;
7. connecting an electrode instrument through a wire, and starting an experiment;
8. at the end of the experiment, the mice were removed.
In summary, 1, the device designs a brand new animal experiment platform with stable form on the basis of its animal bearing platform, the electrode for electrode is dynamically and stably arranged on the bearing platform through screw thread fit and corresponds to three parts of the mouse to be stimulated, compared with the traditional self-made platform, the device is more stable in working, and is not easy to generate dynamic change, so that experimental data collection errors are caused, experimental effect is affected, improvement of experimental quality is facilitated, and unnecessary trouble in experiments can be reduced;
2. secondly, the electrode that the device set up is through threaded connection, for the adjustable scope, applicable different body types size's mouse, through adjusting its position for the electrode chip is better with the position contact mouse that is fit for waiting to stimulate the position, helps promoting the electrode effect of this experiment.
3. The device is additionally provided with a locker with a locked position on the electrode, and the position of the electrode is regulated in a dual mode of a slide rail and screw thread regulation, the slide rail is used for large-scale rapid regulation, the screw thread regulation is used for accurately regulating the small distance of the electrode, the mouse adaptation capability of the whole experiment platform is improved, and convenience is provided for an operator to regulate the position of the electrode; simultaneously, the locker can be used for simultaneously adjusting and locking the sliding rail and adjusting the threads, and the locker can be directly pressed or loosened in actual operation to directly realize the locking adjustment of two degrees of freedom of movement, so that the actual operation flow is reduced, and the adjustment of an operator to the electrode is facilitated.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.
Claims (6)
1. A rodent experimental animal vision electrophysiology detection placement platform, comprising: a carrier plate, an electrode;
the electrode is dynamically connected to the bearing flat plate through a connecting block;
the electrode tip is provided with a stimulation component, and a transition cylinder in threaded connection with the bearing flat plate is arranged in the middle; the transition cylinder is in threaded connection with the connecting block, and a locker which is used for unlocking the locking state of the transition cylinder and the bearing flat plate or the transition cylinder through pressing is arranged at the bottom of the connecting block;
the upper part, the left part or the right part and the lower part of the upper surface of the bearing flat plate are provided with T-shaped groove bodies which are in sliding connection with a connecting block, two sides of the bearing flat plate are respectively fixedly provided with a binding belt and a magic tape for sticking and fixing the binding belt, the upper part of the connecting block penetrates through a threaded hole of a front-back threaded connection transition cylinder of the connecting block, the lower part of the connecting block is a T-shaped component which is in sliding connection with the T-shaped groove body, and the locking device is dynamically arranged in the connecting block and the pressing end of the locking device penetrates to one side of the bottom of the connecting block;
the locking device comprises a side clamping member connected with the pressing end and a thread clamping member dynamically driven to stretch out and draw back by the side clamping member, wherein the side clamping member is arranged in a C-shaped groove in the connecting block, an arc-shaped clamping connector is telescopically clamped into a groove in the side wall of the groove body, the thread clamping member is arranged in a cylindrical groove on one side of the C-shaped groove in the connecting block, the top concave end of the thread clamping member is clamped with a transition cylinder thread, the side clamping member is provided with a driving end matched with the transition end of the thread clamping member in an inclined plane, the side clamping member is compressed inwards, and the driving end is matched with the transition end to drive the thread clamping member to move downwards;
and a reset spring for providing a clamping state holding moment is arranged between the side clamping component and the groove wall of the C-shaped groove and between the threaded clamping component and the cylindrical groove.
2. The rodent visual electrophysiology detection placement platform of claim 1 wherein the electrode further comprises a lead movably connected to the interior cavity of the transition cylinder, the stimulation member movably connected to the transition cylinder by a slot; the electrode is divided into a reference electrode connected to the upper part of the plane of the bearing platform, a recording electrode on the side edge and a grounding electrode on the lower part, the stimulating members of the reference electrode and the recording electrode are electrode clamps capable of being opened movably, and the stimulating members of the grounding electrode are sharp electrode needles.
3. The rodent visual electrophysiology detection placement platform of claim 2, wherein the transition cylinder of the recording electrode is in transition connection with an electrode clip by a three-arm joint belt damped mechanical arm, and the electrode clip is movably connected to the end head of the mechanical arm; the mechanical joint arm joint is a universal joint.
4. The rodent visual electrophysiology detection placement platform of claim 3, wherein the electrode clip comprises an upper clip and a lower clip, the upper clip and the lower clip being hinged at a central location, and a return spring being provided between the lower clip and the electrode clip body.
5. The rodent visual electrophysiology detection placement platform of claim 4, wherein the lower surface of the upper jaw is bonded to the upper surface of the lower jaw and each is provided with an electrode pad, and the wires are connected to the electrode pads on the upper and lower jaws.
6. The method for using the rodent visual electrophysiology detection placement platform according to claims 1-5, wherein the method comprises the following steps of;
(1) Placing the device on a plane;
(2) Placing the anesthetized mice on the platform;
(3) Pressing down the pressing end of the locker;
(4) Sliding the connecting block to a proper position;
(5) Rotating the transition cylinder 22 to adjust the coverage of the electrode until the coverage is suitable for the position of the experimental mouse, and loosening the locker to lock the whole electrode position;
(6) Clamping electrodes at three parts to the parts to be stimulated of the mice;
(7) Connecting an electrode instrument through a wire, and starting an experiment;
(8) At the end of the experiment, the mice were removed.
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| CN202111662804.4A CN114403905B (en) | 2021-12-31 | 2021-12-31 | Rodent experimental animal vision electrophysiology detection placement platform |
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