CN114323783A - Sampling method, sampling assembly and sample analyzer - Google Patents
Sampling method, sampling assembly and sample analyzer Download PDFInfo
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- CN114323783A CN114323783A CN202011061707.5A CN202011061707A CN114323783A CN 114323783 A CN114323783 A CN 114323783A CN 202011061707 A CN202011061707 A CN 202011061707A CN 114323783 A CN114323783 A CN 114323783A
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- 238000005070 sampling Methods 0.000 title claims abstract description 197
- 238000000034 method Methods 0.000 title claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 287
- 238000002156 mixing Methods 0.000 claims abstract description 238
- 239000003153 chemical reaction reagent Substances 0.000 claims description 14
- 238000004140 cleaning Methods 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 239000003085 diluting agent Substances 0.000 claims description 13
- 238000007599 discharging Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 description 37
- 239000011324 bead Substances 0.000 description 28
- 239000000243 solution Substances 0.000 description 15
- 238000004898 kneading Methods 0.000 description 11
- 238000001514 detection method Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000012295 chemical reaction liquid Substances 0.000 description 4
- 210000002700 urine Anatomy 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 239000003219 hemolytic agent Substances 0.000 description 2
- 238000010907 mechanical stirring Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
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Abstract
The application relates to the technical field of medical equipment, and particularly discloses a sampling method, a sampling assembly and a sample analyzer. The sampling method comprises the following steps: before the sampling needle filled with the first liquid leaves the first mixing container, driving the sampling needle to absorb the first mixing liquid in the first mixing container, so that part of a cavity of the sampling needle, which is close to one end of the first mixing container, is filled with the first mixing liquid, wherein the first mixing liquid at least comprises the first liquid and the second liquid; the sampling needle is driven away from the first mixing container. In this way, when in the course of the work of sample analysis appearance or the sampling needle leaves first mixing container, when being destroyed because of surface tension and leading to this spherical globule to drip into or sneak into the first mixing liquid in the first mixing container, can hardly cause the change to the type and the concentration of the first mixing liquid in the first mixing container, consequently, can avoid the influence of spherical globule to the analysis result, improve the rate of accuracy of analysis result.
Description
Technical Field
The present application relates to the field of medical equipment technology, and in particular, to a sampling method, a sampling assembly, and a sample analyzer.
Background
A sample analyzer is an apparatus for analyzing data of a biological sample such as blood or urine.
In the long-term research and development process, the inventor of the application finds that in the actual operation process of the instrument in the prior art, because the sample liquid has surface tension at the liquid outlet of the sampling needle, a spherical liquid bead is formed at the liquid outlet of the sample liquid, and the spherical liquid bead can stay at the liquid outlet for a long time. Because the sample analyzer must have the condition such as mechanical vibration or pipeline extrusion in the course of the work, this can lead to surface tension to be destroyed, and spherical liquid pearl drips and causes the sample volume or sample concentration change to drop to the reaction cup, leads to subsequent analysis result to have serious error.
Disclosure of Invention
Based on this, the application provides a sampling method, a sampling assembly and a sample analyzer, which can improve the accuracy of analysis results.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a sampling method comprising: before the sampling needle filled with the first liquid leaves the first mixing container, driving the sampling needle to absorb the first mixing liquid in the first mixing container, so that part of a cavity of the sampling needle, which is close to one end of the first mixing container, is filled with the first mixing liquid, wherein the first mixing liquid at least comprises the first liquid and the second liquid; the sampling needle is driven away from the first mixing container.
In order to solve the above technical problem, another technical solution adopted by the present application is: there is provided a sampling assembly for performing a sampling method as aforesaid, the sampling assembly comprising: a sampling needle for aspirating at least a first liquid; a first blending container for blending the first liquid and the second liquid to form a first blended solution; the first driving unit is used for driving the sampling needle to absorb the first blending liquid in the first blending container, so that part of a cavity of the sampling needle, which is close to one end of the first blending container, is filled with the first blending liquid; and the second driving unit is connected with the sampling needle and is used for driving the sampling needle to move in the horizontal direction and/or the vertical direction.
In order to solve the above technical problem, the present application adopts another technical solution: a sample analyzer is provided that includes the sampling assembly as described above and a measurement assembly coupled to the sampling assembly.
The beneficial effect of this application is: be different from prior art's condition, this application before the sampling needle that is equipped with first liquid leaves first mixing container, the first mixing liquid in the first mixing container of drive sampling needle absorption to make the sampling needle be close to the partial cavity of first mixing container one end and be equipped with first mixing liquid. Therefore, the spherical liquid beads formed at the liquid outlet under the influence of the surface tension are spherical liquid beads of the first blending liquid, and if the spherical liquid beads are dropped into or mixed into the first blending liquid in the first blending container due to the fact that the surface tension is damaged in the working process of the sample analyzer or when the sampling needle leaves the first blending container, the type and the concentration of the first blending liquid in the first blending container can hardly be changed, so that the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts. Wherein:
FIG. 1 is a schematic flow chart of a first embodiment of the sampling method of the present application;
FIG. 2 is a schematic view of the structure of the sampling needle of the present application;
FIG. 3 is a schematic flow chart of a second embodiment of the sampling method of the present application;
FIG. 4 is a schematic flow chart of a third embodiment of the sampling method of the present application;
FIG. 5 is a schematic flow chart of a fourth embodiment of the sampling method of the present application;
FIG. 6 is a schematic flow chart of a fifth embodiment of the sampling method of the present application;
FIG. 7 is a schematic flow chart of a sixth embodiment of the sampling method of the present application;
FIG. 8 is a schematic structural diagram of a first embodiment of a sampling assembly of the present application;
FIG. 9 is a schematic structural diagram of a second embodiment of the sampling assembly of the present application;
fig. 10 is a schematic structural diagram of a third embodiment of the sampling assembly of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments that can be derived by a person skilled in the art from the embodiments given herein without making any inventive step are within the scope of the present disclosure.
An embodiment of the present application provides a sampling method, please refer to fig. 1 and fig. 8 to 10, the sampling method includes:
s11: before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, the sampling needle 11 is driven to suck the first mixing liquid in the first mixing container 12, so that part of the cavity of the sampling needle 11 close to one end of the first mixing container 12 is filled with the first mixing liquid.
The first uniform mixing liquid at least comprises a first liquid and a second liquid, and the first uniform mixing liquid is a liquid to be detected or a prepared liquid. Optionally, the volume of the first liquid in the first blended liquid is substantially less than the volume of the second liquid in the first blended liquid, e.g., the volume ratio of the first liquid to the second liquid may be 1:100 to 1:500, e.g., 1:100, 1:200, 1:300, 1:400, 1:450, 1: 500.
Optionally, the first liquid may be a sample liquid such as blood, urine, or the like, and the first liquid may also be a reagent or a diluent for preparing the reagent or the diluent; the second liquid may be a reagent or a diluent for pre-reaction with the sample fluid prior to detection. When the first liquid is a sample liquid and the second liquid is a reagent for pre-reaction with the sample liquid before detection, the first mixing container 12 is a cuvette. It will be appreciated that the reagent pre-reacted with the sample fluid before entering the measurement assembly may be various, such as a hemolytic agent, a buffer or a staining agent, and is not limited herein.
Referring to fig. 2, the internal cavity of the sampling needle 11 is an L-shaped hollow cavity, the L-shaped hollow cavity includes a first transverse sub-cavity 101 and a first vertical sub-cavity 102, which are communicated with each other, and the first transverse sub-cavity 101 is close to the first blending container 12. After the sampling needle 11 sucks the first blending liquid in the first blending container 12, the first blending liquid is at least filled to the corner of the L-shaped hollow cavity (i.e. the joint of the first transverse sub-cavity 101 and the first vertical sub-cavity 102). In this way, when the surface tension is destroyed and the first blending liquid in the first transverse sub-cavity 101 drops into the first blending container 12, the first liquid in the first vertical sub-cavity 102 is not mixed into the first blending container 12. It should be noted that the present solution is applicable to sampling needles with any structure, and the structure of the sampling needle 11 in fig. 2 is only an example and is not limited.
In addition, since the inner diameter of the sampling needle 11 is only 0.2 to 0.8mm, even if the surface tension is broken, only a small amount of the first kneading liquid is dropped or mixed from the liquid outlet of the sampling needle 11 into the first kneading container 12. The volume of the first mixing liquid sucked by the sampling needle 11 can be set according to the user's requirement, and is not limited herein.
S12: the sampling needle 11 is driven away from the first mixing container 12.
Specifically, after a part of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid, the sampling needle 11 is driven to leave the first mixing container 12. The spherical liquid bead formed at the liquid outlet of the sampling needle 11 under the influence of the surface tension is the spherical liquid bead of the first mixing liquid, and if the spherical liquid bead is dropped into or mixed into the first mixing liquid in the first mixing container 12 due to the damage of the surface tension in the working process of the sample analyzer or when the sampling needle 11 leaves the first mixing container 12, the type and concentration of the first mixing liquid in the first mixing container 12 can hardly be changed.
Different from the situation in the prior art, before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, the sampling needle 11 is driven to suck the first mixing liquid in the first mixing container 12, so that part of the cavity of the sampling needle 11 close to one end of the first mixing container 12 is filled with the first mixing liquid. In this way, the spherical liquid beads formed at the liquid outlet under the influence of the surface tension are spherical liquid beads of the first blending liquid, and if the spherical liquid beads are dropped into or mixed into the first blending liquid in the first blending container 12 due to the damage of the surface tension in the working process of the sample analyzer or when the sampling needle 11 is separated from the first blending container 12, the type and concentration of the first blending liquid in the first blending container 12 are hardly changed, so that the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result is improved.
In some embodiments, referring to fig. 3 and fig. 8-10, before S11, the method further includes:
s13: the sampling needle 11 is driven into the first mixing container 12, and the liquid outlet of the sampling needle 11 is positioned below the liquid level of the first mixing container 12 filled with the second liquid.
Specifically, before step S13, the sampling needle 11 sucks the first liquid in the test tube (not shown), and after the sampling needle 11 sucks the first liquid in the test tube, the outer wall of the sampling needle 11 is cleaned. At the same time, the second liquid is injected into the first kneading container 12. The sampling needle 11 is then extended into the second liquid in the first mixing container 12.
S14: the sampling needle 11 is driven to inject the first liquid into the first kneading container 12 to form the first kneading liquid.
Specifically, the sampling needle 11 may inject the first liquid below the liquid level of the first kneading vessel 12 in a plurality of times. In addition, a mixing mechanism (e.g., a stirring magnet or a mechanical stirring rod) (not shown) may be installed in the first mixing container 12 to improve the mixing efficiency of the first liquid and the second liquid.
In some embodiments, referring to fig. 4 and fig. 8-10, when there are at least two mixing containers in the sample analyzer, after step S12, the method further includes:
s15: the sampling needle 11 containing the first liquid is driven into the second mixing container 13, and the liquid outlet of the sampling needle 11 is positioned below the liquid level of the second mixing container 13 containing the third liquid.
Specifically, before step S15, the third liquid is injected into the second kneading container 13. The sampling needle 11 is then extended into the third liquid in the second mixing container 13.
S16: and driving the sampling needle 11 to inject the first liquid into the second blending container 13 to form second blending liquid, wherein the second blending liquid at least comprises the first liquid and the third liquid.
Specifically, the sampling needle 11 may inject the first liquid below the liquid level of the second kneading vessel 13 in a plurality of times. In addition, a mixing mechanism (for example, a stirring magnet or a mechanical stirring rod) (not shown) may be installed in the second mixing container 13 to improve the mixing efficiency of the first liquid and the third liquid.
S17: before the sampling needle 11 filled with the first liquid leaves the second mixing container 13, the sampling needle 11 is driven to suck the second mixing liquid in the second mixing container 13, so that part of the cavity of the sampling needle 11 close to one end of the second mixing container 13 is filled with the second mixing liquid.
The second uniform mixing liquid at least comprises a first liquid and a third liquid, and the first uniform mixing liquid is a liquid to be detected or a prepared liquid. Optionally, the volume of the first liquid in the second blending liquid is much smaller than the volume of the third liquid in the second blending liquid, e.g., the volume ratio of the first liquid to the third liquid may be 1:100 to 1:500, e.g., 1:100, 1:200, 1:300, 1:400, 1:450, 1: 500.
Optionally, the first liquid may be a sample liquid such as blood, urine, or the like, and the first liquid may also be a reagent or a diluent for preparing the reagent or the diluent; the third liquid may be a reagent or diluent for pre-reaction with the sample liquid prior to detection. When the first liquid is a sample liquid and the third liquid is a reagent for pre-reaction with the sample liquid before detection, the second mixing container 13 is a cuvette. It is understood that the reagent pre-reacted with the sample solution before the detection may be various reagents, such as a hemolytic agent or a buffer, and is not limited herein.
It should be noted that, referring to fig. 2, the internal cavity of the sampling needle 11 is an L-shaped hollow cavity, the L-shaped hollow cavity includes a first transverse sub-cavity 101 and a first vertical sub-cavity 102, which are communicated with each other, and the first transverse sub-cavity 101 is close to the second mixing container 13. After the sampling needle 11 sucks the second mixing liquid in the second mixing container 13, the second mixing liquid is at least filled to the corner of the L-shaped hollow cavity (i.e. the joint of the first transverse sub-cavity 101 and the first vertical sub-cavity 102). In this way, when the surface tension is broken and the second blending liquid in the first transverse sub-cavity 101 drops into the second blending container 13, the first liquid in the first vertical sub-cavity 102 is not mixed into the second blending container 13.
S18: the sampling needle 11 is driven away from the second mixing container 13.
Specifically, after a part of the cavity of the sampling needle 11 near one end of the second mixing container 13 is filled with the second mixing liquid, the sampling needle 11 is driven to leave the second mixing container 13. The spherical liquid bead formed at the liquid outlet of the sampling needle 11 under the influence of the surface tension is a spherical liquid bead of the second mixing liquid, and if the spherical liquid bead is dropped into or mixed into the second mixing liquid in the second mixing container 13 due to the damage of the surface tension in the working process of the sample analyzer or when the sampling needle 11 leaves the second mixing container 13, the type and concentration of the second mixing liquid in the second mixing container 13 can hardly be changed.
Through the mode, before sampling needle 11 that this application was equipped with first liquid left second mixing container 13, drive sampling needle 11 and absorb the second mixing liquid in the second mixing container 13 to make sampling needle 11 be close to the partial cavity of second mixing container 13 one end and be equipped with second mixing liquid. In this way, the spherical liquid beads formed at the liquid outlet under the influence of the surface tension are spherical liquid beads of the second mixing liquid, and if the spherical liquid beads are dropped into or mixed into the second mixing liquid in the second mixing container 13 due to the damage of the surface tension in the working process of the sample analyzer or when the sampling needle 11 is separated from the second mixing container 13, the type and concentration of the second mixing liquid in the second mixing container 13 are hardly changed, so that the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result can be improved.
Referring to fig. 5 and fig. 8-10, in some embodiments, before step S15, the method further includes:
s19: the first homogenate liquid and a part of the first liquid in the sampling needle 11 are discharged.
Since the composition and/or concentration of the first homogeneous liquid is different from that of the second homogeneous liquid, the first homogeneous liquid in the sampling needle 11 needs to be discharged before step S15, and in addition, since the first liquid is contained in the sampling needle 11, the inner wall of the sampling needle 11 cannot be cleaned, and the purpose of flushing the first transverse subcavity 101 is achieved by discharging part of the first liquid.
The first mixing fluid and a part of the first fluid in the sampling needle 11 can be discharged through the first mixing container 12 or the swab (not shown).
After step S19, the process proceeds to step S20 or S21.
S20: the outer wall of the sampling needle 11 is cleaned with a cleaning liquid.
Since the liquid outlet of the sampling needle 11 is located below the liquid level of the first mixing container 12 containing the second liquid in step S13, the second liquid and the first mixed liquid adhere to the outer wall of the sampling needle 11, and in order to avoid contaminating the second mixed liquid, it is necessary to clean the outer wall of the sampling needle 11 with a cleaning liquid.
S21: the outer surface of the sampling needle 11 is air-dried.
It will be appreciated that the outer surface of the sampling needle 11 is air dried to eliminate the effect of the cleaning fluid or other reagents on the second mixing fluid.
If a plurality of blending containers exist in the sample analyzer and need to be subjected to sample separation, the steps S15-S21 can be repeated to achieve the purpose of multiple sample separation.
Referring to fig. 6-7 and 8-10, in some embodiments, after step S12 or S18, the method further includes:
s22: the outer wall and the inner wall of the sampling needle 11 are cleaned with a cleaning liquid.
Specifically, after the sampling needle 11 leaves the first kneading container 12 or the second kneading container 13, if the sampling needle 11 is not in the state of sample separation, the outer wall and the inner wall of the sampling needle 11 are cleaned with a cleaning liquid.
Referring to fig. 8, the sampling assembly 10 according to an embodiment of the present invention is a sampling assembly 10 for performing the sampling method according to the above embodiment, where the sampling assembly 10 includes: a sampling needle 11, a first mixing container 12, a first drive unit 14 and a second drive unit (not shown).
The sampling needle 11 is used to aspirate at least a first liquid. It should be noted that, referring to fig. 2, the internal cavity of the sampling needle 11 is an L-shaped hollow cavity, the L-shaped hollow cavity includes a first transverse sub-cavity 101 and a first vertical sub-cavity 102, which are communicated with each other, and the first transverse sub-cavity 101 is close to the first blending container 12. After the sampling needle 11 sucks the first blending liquid in the first blending container 12, the first blending liquid is at least filled to the corner of the L-shaped hollow cavity (i.e. the joint of the first transverse sub-cavity 101 and the first vertical sub-cavity 102). In this way, when the surface tension is destroyed and the first blending liquid in the first transverse sub-cavity 101 drops into the first blending container 12, the first liquid in the first vertical sub-cavity 102 is not mixed into the first blending container 12. It should be noted that the present solution is applicable to sampling needles with any structure, and the structure of the sampling needle 11 in fig. 2 is only an example and is not limited.
The first blending container 12 is used to blend the first liquid and the second liquid to form a first blended solution.
The first drive unit 14 is used to provide the liquid in the sampling needle with drive power.
Specifically, the first drive unit 14 includes: a first pressure supply device 141 and a control circuit 143. The first pressure supply device 141 is connected to the sampling needle 11 through the fourth electromagnetic valve 174, and the control circuit 143 is electrically connected to the first pressure supply device 141 and controls the first pressure supply device 141. The control circuit 143 is configured to control the first pressure supply device 141 to provide driving power for the liquid in the sampling needle 11 before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, so that the sampling needle 11 can suck the first mixing liquid in the first mixing container 12, and a part of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid. The first pressure supply device 141 may be an air pump, a liquid pump, a syringe, a negative pressure tank, etc., and is not limited thereto.
The sampling needle 11 is fixed on a second driving unit, and the second driving unit is used for driving the sampling needle 11 filled with the first liquid to move in the horizontal direction and/or the vertical direction, so that the sampling needle 11 enters or leaves the first driving unit 14.
Different from the prior art, the control circuit 143 of this application is used for before the sampling needle 11 that is equipped with first liquid leaves first mixing container 12, and the first pressure device 141 that supplies of control pulling is first supplies pressure device 141 in order to drive sampling needle 11 and absorb first mixing liquid in first mixing container 12 to make the partial cavity that sampling needle 11 is close to first mixing container 12 one end be equipped with first mixing liquid. In this way, the spherical liquid beads formed at the liquid outlet under the influence of the surface tension are spherical liquid beads of the first blending liquid, and if the spherical liquid beads are dropped into or mixed into the first blending liquid in the first blending container 12 due to the damage of the surface tension in the working process of the sample analyzer or when the sampling needle 11 is separated from the first blending container 12, the type and concentration of the first blending liquid in the first blending container 12 are hardly changed, so that the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result is improved.
Referring to fig. 9, in some embodiments, the sampling assembly 10 further includes: a second kneading vessel 13.
The second mixing vessel 13 is used to mix the first liquid and the third liquid to form a second mixed solution.
The second driving unit is further configured to drive the sampling needle 11 filled with the first liquid to enter the second blending container 13, and enable the liquid outlet of the sampling needle 11 to be located below the liquid level of the second blending container 13.
The control circuit 143 is configured to control the first pressure supply device 141 to provide driving power for the liquid in the sampling needle 11 before the sampling needle 11 filled with the first liquid leaves the second mixing container 13, so that the sampling needle 11 can suck the second mixing liquid in the second mixing container 13, and a part of the cavity of the sampling needle 11 near one end of the second mixing container 13 is filled with the second mixing liquid. The first pressure supply device 141 may be an air pump, a liquid pump, a syringe, a negative pressure tank, etc., and is not limited thereto.
The second driving unit is also used for driving the sampling needle 11 to leave the second blending container 13.
The sampling assembly 10 further includes: a liquid storage container for storing diluent or other reagents required by the reaction, and the first mixing container 12 and/or the second mixing container 13 are/is controllably connected with the liquid storage container.
With continued reference to FIG. 9, the reservoir may be a reaction reservoir 15. The reaction liquid pool 15 is used for storing the reaction liquid. In some embodiments, the first solution and the second solution may be reaction solutions with the same type and concentration, in this case, the reaction solution pool 15 is connected to the first blending container 12 through the first electromagnetic valve 171, the reaction solution pool 15 is connected to the second blending container 13 through the second electromagnetic valve 172, and the reaction solution pool 15 is connected to the cleaning unit 18 through the third electromagnetic valve 173, so as to inject the reaction solutions into the first blending container 12, the second blending container 13, and the cleaning unit 18, respectively.
Further, with reference to fig. 9, the first blending container 12 and the second blending container 13 are respectively communicated with the second pressure supply device 121, and the second pressure supply device 121 is used for providing driving power for the liquid in the first blending container 12 and the second blending container 13, so as to inject the reaction liquid in the reaction liquid pool 15 into the first blending container 12 and the second blending container 13 when the first electromagnetic valve 171 and the second electromagnetic valve 172 are opened. The second pressure supply device 121 may be an air pump, a liquid pump, a syringe, a negative pressure tank, etc., and is not limited thereto.
Referring to fig. 10, in certain embodiments, the sampling assembly 10 further comprises: a cleaning unit 18. The cleaning unit 18 is used to clean the outer wall of the sampling needle 11 with a cleaning liquid. Further, the washing unit 18 communicates with the third pressure supply device 19 through the sixth solenoid valve 176, and the third pressure supply device 19 is used for supplying driving power to the washing liquid in the washing unit 18.
With continued reference to fig. 10, in some embodiments, the first pressure supply device 141 may be a syringe, the reservoir may be a diluent reservoir 16, the diluent reservoir 16 is used for storing diluent, and the diluent reservoir 16 is connected to the inlet of the syringe through a fifth solenoid valve 175 to inject the diluent into the syringe, so as to clean the inner wall of the sampling needle 11 through the syringe.
The embodiment of the application provides a sample analyzer, and the sample analyzer can be used for analyzing biological samples, and the biological samples can be blood, urine and the like. The sample analyzer may further include a driving assembly, a measuring assembly, a waste liquid processing assembly, and a controller, in addition to the sampling assembly 10 in the above embodiments. The drive assembly is used to drive various flow paths (including gas and liquid paths) in the sample analyzer. The measuring assembly is connected with the sampling assembly and used for detecting the first blending liquid to form detection information. The waste liquid treatment assembly is used for collecting and discharging waste liquid in the sample analyzer. The controller is used for controlling the work flow of the sample analyzer and processing the detection information to obtain a result.
Different from the situation in the prior art, before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, the sampling needle 11 is driven to suck the first mixing liquid in the first mixing container 12, so that part of the cavity of the sampling needle 11 close to one end of the first mixing container 12 is filled with the first mixing liquid. In this way, the spherical liquid beads formed at the liquid outlet under the influence of the surface tension are spherical liquid beads of the first blending liquid, and if the spherical liquid beads are dropped into or mixed into the first blending liquid in the first blending container 12 due to the damage of the surface tension in the working process of the sample analyzer or when the sampling needle 11 is separated from the first blending container 12, the type and concentration of the first blending liquid in the first blending container 12 are hardly changed, so that the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result is improved.
In the description of the present application, it should be noted that the terms "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are only for convenience in describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and operate, and thus, should not be construed as limiting the present application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (10)
1. A sampling method, characterized in that the sampling method comprises:
before a sampling needle filled with first liquid leaves a first mixing container, driving the sampling needle to suck the first mixing liquid in the first mixing container so that a part of a cavity of the sampling needle, which is close to one end of the first mixing container, is filled with the first mixing liquid, wherein the first mixing liquid at least comprises the first liquid and second liquid;
and driving the sampling needle to leave the first blending container.
2. The sampling method of claim 1, wherein prior to the sampling needle containing the first liquid exiting the first blending container, the method further comprises:
driving the sampling needle to enter the first mixing container, wherein a liquid outlet of the sampling needle is positioned below the liquid level of the first mixing container filled with the second liquid;
and driving the sampling needle to inject the first liquid into the first blending container so as to form the first blending liquid.
3. The sampling method of claim 1, wherein after said driving said sampling needle out of said first blending container, said method further comprises:
driving the sampling needle filled with the first liquid into a second mixing container, wherein a liquid outlet of the sampling needle is positioned below the liquid level of the second mixing container filled with a third liquid;
driving the sampling needle to inject the first liquid into the second blending container to form second blending liquid, wherein the second blending liquid at least comprises the first liquid and the third liquid;
before the sampling needle filled with the first liquid leaves the second mixing container, driving the sampling needle to suck the second mixing liquid in the second mixing container, so that part of a cavity of the sampling needle, which is close to one end of the second mixing container, is filled with the second mixing liquid;
and driving the sampling needle to leave the second mixing container.
4. The sampling method according to any one of claims 1 to 3,
the volume of the first liquid in the first blending liquid is much smaller than the volume of the second liquid in the first blending liquid;
the volume of the first liquid in the second blending liquid is much smaller than the volume of the third liquid in the second blending liquid.
5. The sampling method of claim 3, wherein prior to said driving the sampling needle containing the first liquid into the second blending container, the sampling method further comprises:
discharging the first blending liquid and part of the first liquid in the sampling needle;
and cleaning the outer wall of the sampling needle by adopting a cleaning solution, or air-drying the outer wall of the sampling needle.
6. The sampling method of claim 1 or 3, wherein after driving the sampling needle away from the first blending container or the second blending container, the sampling method further comprises:
and cleaning the outer wall and the inner wall of the sampling needle by adopting cleaning liquid.
7. A sampling assembly for performing the method of any of claims 1-6, the sampling assembly comprising:
a sampling needle for aspirating at least a first liquid;
a first blending container for blending the first liquid and the second liquid to form a first blended solution;
the first driving unit is used for driving the sampling needle to absorb the first blending liquid in the first blending container, so that part of the cavity of the sampling needle, which is close to one end of the first blending container, is filled with the first blending liquid;
and the second driving unit is connected with the sampling needle and is used for driving the sampling needle to move in the horizontal direction and/or the vertical direction.
8. The sampling assembly of claim 7, further comprising:
a second blending container for blending the first liquid and the third liquid to form a second blended solution;
the second driving unit is used for driving the sampling needle filled with the first liquid to enter or leave the first driving unit, and the second driving unit is also used for driving the sampling needle filled with the first liquid to enter the second blending container, and enabling the liquid outlet of the sampling needle to be positioned below the liquid level of the second blending container;
the first driving unit is also used for driving the sampling needle to suck the second blending liquid in the second blending container, so that part of the cavity of the sampling needle close to one end of the second blending container is filled with the second blending liquid.
9. The sampling assembly of claim 8, further comprising:
the liquid storage container is used for storing diluent or other reagents required by the reaction, and the first blending container and/or the second blending container are/is controllably connected with the liquid storage container.
10. A sample analyser comprising a sampling assembly according to any one of claims 7 to 9 and a measurement assembly connected to the sampling assembly.
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