CN114292746A

CN114292746A - Automatic blood and liquid distribution system for cell culture

Info

Publication number: CN114292746A
Application number: CN202111664900.2A
Authority: CN
Inventors: 陆文斌
Original assignee: Changzhou Wujin Peoples Hospital
Current assignee: Changzhou Wujin Peoples Hospital
Priority date: 2021-12-31
Filing date: 2021-12-31
Publication date: 2022-04-08

Abstract

The invention provides an automatic blood and liquid distribution system for cell culture, which comprises a liquid injection device, a rocker arm support, a metering type distribution support and a metering type shaking device, wherein the liquid injection device comprises a liquid injection vessel and a main hose, the rocker arm support is used for adjusting the spatial position of the liquid injection vessel, one end of the main hose is communicated with the liquid injection vessel, the other end of the main hose is connected with a plurality of branch hoses or culture bags, the culture bags are arranged in the metering type shaking device, injection needles are arranged on the branch hoses, a plurality of centrifuge tubes are arranged in the metering type distribution support, the injection needles are abutted against the side walls of the centrifuge tubes and are in one-to-one correspondence with the centrifuge tubes in number, and regulators for adjusting the section sizes of the branch hoses are arranged in the metering type distribution support. The invention adopts a metering type distribution bracket to slowly inject the blood cells without plasma into a centrifuge tube containing lymphocyte separation liquid, and controls the liquid injection speed of the branch hose by changing the section size of the branch hose, thereby realizing the liquid level in each centrifuge tube to be level.

Description

Automatic blood and liquid distribution system for cell culture

Technical Field

The invention relates to the technical field of medical instruments, in particular to an automatic blood and liquid distribution system for cell culture.

Background

In the cell culture process, particularly in the cell culture involved in the immunotherapy process, the liquid operations such as blood cell separation and medium replacement are the work that the experiment operator needs to perform every day. During this process, the operator encounters a number of problems.

First, during cell culture, large volumes of media are often filled into culture bags. Previously, a 50ml syringe with the piston removed was used as a funnel and mounted on a support made of plexiglas or stainless steel. The self-made bracket is inconvenient to use because the self-made bracket can not be adjusted up, down, left, right, front and back according to the intention of a user. The culture medium fluid replacement is carried out every day as the conventional work of cell culture, and an improper bracket seriously influences the working efficiency and even can bring harm to the health of workers due to inconvenient posture. Therefore, there is a need for a rocker arm stand that is adjustable in attitude, easy to sterilize, and simple in construction.

Secondly, in previous cell culture work, doctors often need to extract mononuclear cells (PBMCs) in blood and then perform culture or other operations. In this process, the blood cells from which the plasma is removed are first required to be slowly poured into a 50ml centrifuge tube containing 20ml of lymphocyte separation fluid, and the blood cells are required to be stably kept above the lymphocyte separation fluid, and the layering is clear and can not be confused. This imposes strict requirements on the operating technique of the laboratory personnel, not only for special training, but also for a long incubation time to be able to perform this step excellently. In the earlier filed patent CN201720388419.8, "a blood dispenser dedicated to cell culture", the difficulty of this operation was reduced and the quality of the isolated cells was maintained high. However, in practical use, the device is cumbersome, not only needs to be powered on, but also occupies a large area, so that the operating table which can catch the elbow is more tense. In addition, because of the different densities of blood and separation fluid, balancing is still performed prior to centrifugation. Therefore, there is a need for a blood dispensing device that is simple in construction, easy to operate, does not require electrical cabling, and is capable of automatic balancing.

Third, subculture or replenisher is a routine procedure in cell culture, and laboratories are performed many times a day. For small volume flasks, fluid replacement is convenient because each flask has little media to replenish and can be accomplished easily with a pipette. However, for large-volume culture bags or cell factories, the liquid replenishment is laborious, and the liquid replenishment can reach hundreds of milliliters each time, and the liquid replenishment can be completed by using a pipettor for many times. This not only makes the large volume fluid replacement inefficient, but also increases the likelihood of cell contamination. Conventionally, a 50ml syringe with a piston removed was used as a funnel, connected to a culture bag, and then directly perfused with a culture medium. However, this also entails a series of problems, such as the inability to accurately calculate the volume of medium to be filled; it is inconvenient to shake the culture bag while replenishing the culture medium to mix the culture medium uniformly; the labor is consumed, and the like. Therefore, it is necessary to develop a liquid mixing platform with weighing function for cell culture.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: to overcome the deficiencies of the prior art, the present invention provides an automatic blood and fluid dispensing system for cell culture.

The technical scheme adopted for solving the technical problems is as follows: an automatic blood and liquid distribution system for cell culture comprises a liquid injection device, a rocker arm bracket, a metering type distribution bracket and a metering type shaking device, the liquid injection device comprises a liquid injection vessel and a main hose, the rocker arm bracket is used for adjusting the spatial position of the liquid injection vessel, one end of the main hose is communicated with the liquid injection vessel, the other end of the main hose is connected with a plurality of sub-hoses or culture bags, the culture bag is arranged in the metering type shaking device, the metering type shaking device is used for shaking and weighing the liquid in the culture bag, the distribution hose is provided with an injection needle, the metering distribution bracket is internally provided with a plurality of centrifuge tubes, the syringe needle support locate on the centrifuging tube lateral wall position and quantity and centrifuging tube one-to-one, be equipped with in the metering type distribution support and be used for adjusting the regulator of each branch hose cross-sectional size.

Locate the injection household utensils in the rocker arm support for when conveniently annotating the liquid, the spatial position of adjustment injection household utensils more is fit for experimenter's use habit, convenient operation. When the blood cells with the plasma removed are injected into a centrifuge tube containing a separation liquid, the blood cells flow into a main hose from an injector dish, then are shunted through a plurality of sub-hoses and respectively flow into each centrifuge tube, and an injection needle head is abutted against the inner wall surface of the centrifuge tube, so that the blood cells are stably kept above the lymphocyte separation liquid, and are clearly layered and not confused; the size of the section of each sub-hose is adjusted through an adjuster, so that the speed of injecting the blood cell suspension into each centrifugal tube is adjusted to realize balancing, and the liquid level height in each centrifugal tube is the same finally. When liquid is supplemented into the large-volume culture bag, the culture bag is connected with the main hose, then culture medium is directly filled into the culture bag through an injection vessel, the metering type shaking device shakes the culture bag to uniformly mix the culture medium, and through the weighing function of the metering type shaking device, when the mixed liquid in the culture bag reaches the specified weight, an operator stops adding the culture medium into the injection vessel.

Further, metering type distribution support includes the body frame, it places the hole to be equipped with a plurality of centrifuging tubes on the body frame, it is equipped with miniature electronic scale to place the hole bottom, it is equipped with the mount to place the hole top, the mount front end be equipped with syringe needle complex caulking groove, the mount rear end is equipped with the regulator.

A plurality of centrifuging tubes are put into respectively by operating personnel and are placed downtheholely, and miniature electronic scale is used for weighing to the liquid in the centrifuging tube, sets up the caulking groove on the mount and is used for fixed syringe needle position, replaces the staff to hold syringe needle and annotate liquid in to the centrifuging tube, to operating personnel technical requirement greatly reduced. The fixed frame is provided with an adjuster used for adjusting the section size of the sub-hose, thereby adjusting the liquid injection speed of each sub-hose to each centrifugal pipe.

Further, divide the liquid speed of annotating of hose for automatically regulated, the regulator includes frame type support and locates the adjusting gear in the frame type support, be equipped with the spout on frame type support both sides wall, the spout is followed to frame type support front end direction slope from frame type support rear end to frame type support diapire direction, the adjusting gear both sides be equipped with connecting portion with spout sliding connection, divide the hose to locate between adjusting gear and the frame type support diapire, be equipped with on the frame type support roof with adjusting gear meshing's rack, be equipped with driving motor on the adjusting gear connecting portion, the driving motor output with the coaxial setting of connecting portion, driving motor casing pastes and locates on the outer wall of frame type support.

The adjusting gear is meshed with the rack on the top wall of the frame-shaped support, when the driving motor drives the adjusting gear to rotate forwards or reversely, the adjusting gear moves back and forth on the rack, and the sliding groove is used for limiting the position of the adjusting gear and ensuring that the adjusting gear is always meshed with the rack; the sub-hose is arranged between the adjusting gear and the bottom wall of the frame-shaped support, and when the adjusting gear moves towards the front end from the rear end of the frame-shaped support, the sub-hose is gradually extruded and flattened, the section size of the sub-hose is changed, and therefore the liquid injection speed of the sub-hose is controlled.

Further, in the regulator is installed into to the branch hose for the convenience, avoid syringe needle and regulator touching simultaneously, frame type support diapire can be dismantled with frame type support lateral wall and be connected. Through with the regulator split, expose frame type support diapire, fix the syringe needle in the caulking groove, divide the hose to pave on frame type support diapire, then with frame type support lateral wall and diapire lock, accomplish the installation of branch hose, whole process accords with safe operation and absolute aseptic requirement, and the syringe needle can not contact any article.

Further, be close to or keep away from the centrifuging tube internal face for syringe needle on the control mount, metering type distribution support is still including adjusting the support and adjusting the pole, it locates to adjust the support body frame one side, it is articulated with the horizontal direction to adjust the support lower extreme articulated point department is equipped with and is used for the drive adjust the support along pin joint pivoted accommodate motor, it is close to or keeps away from along the pin joint swing to adjust the support lower extreme the body frame, it is close to with the mount to adjust support upper end one side of regulator is articulated, be equipped with the adjustment tank along length direction on the regulation support lateral wall, it is articulated with the mount intermediate position to adjust pole one end, and the other end is located in the adjustment tank with adjustment tank sliding connection, it adjusts the gliding drive arrangement of pole along the adjustment tank to be equipped with the drive on the regulation support.

The adjusting motor is used for driving the adjusting bracket to swing and driving the fixing frame at the upper end of the adjusting bracket to be close to or far away from the centrifugal tube; the adjusting rod slides along the adjusting groove under the driving of the driving device, so that the angle between the fixing frame and the adjusting bracket is adjusted.

Further, for the position of annotating the liquid household utensils on the free adjustment rocker arm support, rocker arm support includes base and multistage articulated support arm in proper order, be equipped with the screw of adjusting the elasticity on the support arm pin joint, it is provided with the stand to rotate along vertical direction on the base, the support arm of below with the stand is articulated along the horizontal direction, the support arm of the top be used for with annotate the liquid household utensils and connect.

Furthermore, the uppermost support arm is provided with a clamp matched with the outer wall surface of the liquid injection vessel.

Furthermore, in order to enable the metering type shaking device to have the functions of shaking and weighing, the metering type shaking device comprises a tray and an electronic scale, the tray is arranged above the working surface of the electronic scale and is hinged with the electronic scale, and a swinging assembly for driving the tray to swing along a hinged point is arranged on the electronic scale.

Furthermore, in order to drive the culture bags arranged on the tray to swing, the swing assembly comprises a servo motor and a reset spring, the servo motor and the reset spring are arranged on the working surface of the electronic scale and are respectively arranged on two sides of a hinge joint of the tray and the electronic scale, a cam is arranged on the output end of the servo motor, the outer peripheral wall of the cam is matched with the lower end face of the tray, and the servo motor drives the cam to rotate and drive the tray to swing along the hinge joint.

Servo motor drives the cam and rotates, and the cam periphery wall supports the tray lower extreme, drives the tray and follows the pin joint swing when the tray rotates, and reset spring is used for ensuring that tray lower terminal surface hugs closely on the cam. The electronic scale is used for weighing the servo motor, the cam, the reset spring, the tray and the culture bag arranged on the tray, the peeling function of the electronic scale is used before weighing, the weight of the servo motor, the cam, the reset spring, the tray and the culture bag is reduced, and then the liquid in the culture bag is weighed.

Further, be equipped with the segmental arc that is used for the buffering on the cam periphery wall, the distance of segmental arc to cam rotation center is unchangeable. The arc section is the buffering section, and when cam arc section and tray cooperation, the tray stopped swinging, firstly for accurate weighing, secondly for reducing inertia.

The invention has the beneficial effects that: according to the automatic blood and liquid distribution system for cell culture, the rocker arm support is adopted, the position of the liquid injection vessel can be freely adjusted, the use habit of operators is met, and the working efficiency is improved; the blood cells with the plasma removed are slowly injected into the centrifuge tube containing the lymphocyte separation liquid by adopting the metering type distribution bracket, the blood cells are stably kept above the lymphocyte separation liquid and are clearly layered, and the liquid injection speed of the sub-hose is controlled by changing the section size of the sub-hose, so that the liquid level in each centrifuge tube is parallel and level; adopt even device of metering type shake convenient shaking culture bag in the time of the fluid infusion in order to the mixing culture medium, can accurately calculate the total amount of the culture medium of filling.

Drawings

The invention is further illustrated by the following figures and examples.

FIG. 1 is a schematic structural diagram of a preferred embodiment of the present invention;

FIG. 2 is a schematic structural view of the liquid injection device;

FIG. 3 is a schematic structural view of the rocker arm stand;

FIG. 4 is a schematic structural view of a metered dispensing holder;

FIG. 5 is a schematic view of the structure of the fixing frame and the adjusting bracket;

FIG. 6 is an enlarged schematic cross-sectional view A of FIG. 5;

FIG. 7 is a schematic view of the engagement of the adjuster with the mount;

FIG. 8 is a schematic cross-sectional view of the regulator;

FIG. 9 is a schematic view of the structure of the metering shake-up device;

FIG. 10 is a side view of a metered shake-up device;

fig. 11 is a structural schematic view of the cam.

In the figure: 1. the liquid injection device comprises a liquid injection device 11, a liquid injection vessel 12, a main hose 13, a branch hose 14, an injection needle 2, a rocker arm support 21, a base 22, a support arm 23, a column 24, a clamp 3, a metering distribution support 31, a main frame 311, a placing hole 312, a miniature electronic scale 32, a fixing frame 321, a caulking groove 33, a regulator 331, a frame-shaped support 332, an adjusting gear 333, a sliding groove 334, a rack 34, an adjusting support 35, an adjusting rod 351, an adjusting groove 36, a cylinder 4, a metering shaking device 41, a tray 42, an electronic scale 43, a reset spring 44, a cam 441, an arc section 45, a servo motor 5, a centrifuge tube 6 and a culture bag.

Detailed Description

The present invention will now be described in detail with reference to the accompanying drawings. This figure is a simplified schematic diagram, and merely illustrates the basic structure of the present invention in a schematic manner, and therefore it shows only the constitution related to the present invention.

As shown in fig. 1 and 2, the automatic blood and liquid distribution system for cell culture of the present invention comprises a liquid injection device 1, a rocker arm support 2, a metering type distribution support 3 and a metering type shaking device 4, wherein the liquid injection device 1 comprises a liquid injection vessel 11 and a main hose 12, the rocker arm support 2 is used for adjusting the spatial position of the liquid injection vessel 11, one end of the main hose 12 is communicated with the liquid injection vessel 11, the other end of the main hose is connected with a plurality of branch hoses 13 or connected with a culture bag 6, the culture bag 6 is arranged in the metering type shaking device 4, the metering type shaking device 4 is used for shaking and weighing the liquid in the culture bag 6, the branch hoses 13 are provided with injection needles 14, a plurality of centrifuge tubes 5 are arranged in the metering type distribution support 3, the injection needles 14 are abutted against the side walls of the centrifuge tubes 5 and are in one-to-one correspondence with the centrifuge tubes 5 in number, an adjuster 33 for adjusting the cross-sectional size of each sub-hose 13 is arranged in the metering distribution support 3.

As shown in fig. 4 and 7, the metering distribution support 3 includes a main frame 31, a plurality of centrifugal tube 5 placing holes 311 are provided on the main frame 31, a micro electronic scale 312 is provided at the bottom of the placing holes 311, a fixing frame 32 is provided above the placing holes 311, an insertion groove 321 that is engaged with the injection needle 14 is provided at the front end of the fixing frame 32, and the regulator 33 is provided at the rear end of the fixing frame 32.

As shown in fig. 8, the regulator 33 includes a frame-shaped support 331 and an adjusting gear 332 disposed in the frame-shaped support 331, sliding grooves 333 are disposed on two side walls of the frame-shaped support 331, the sliding grooves 333 are inclined toward a bottom wall of the frame-shaped support 331 from a rear end of the frame-shaped support 331 toward a front end of the frame-shaped support 331, connecting portions are disposed on two sides of the adjusting gear 332 and slidably connected to the sliding grooves 333, the sub-hose 13 is disposed between the adjusting gear 332 and the bottom wall of the frame-shaped support 331, the bottom wall of the frame-shaped support 331 is detachably connected to a side wall of the frame-shaped support, a rack 334 engaged with the adjusting gear 332 is disposed on a top wall of the frame-shaped support 331, a driving motor is disposed on the connecting portion of the adjusting gear 332, an output end of the driving motor is disposed coaxially with the connecting portions, and a casing of the driving motor is attached to an outer wall of the frame-shaped support 331.

As shown in fig. 5 and 6, the metering and dispensing support 3 further includes an adjusting support 34 and an adjusting rod 35, the adjusting support 34 is disposed on one side of the main frame 31, a lower end of the adjusting support 34 is hinged to the horizontal direction, an adjusting motor for driving the adjusting support 34 to rotate along a hinge point is disposed at the hinge point of the lower end of the adjusting support 34, the lower end of the adjusting support 34 swings along the hinge point to approach or depart from the main frame 31, an upper end of the adjusting support 34 is hinged to one side of the fixing frame 32 close to the adjuster 33, an adjusting groove 351 is disposed on a side wall of the adjusting support 34 along the length direction, one end of the adjusting rod 35 is hinged to a middle position of the fixing frame 32, the other end of the adjusting rod is disposed in the adjusting groove 351 and slidably connected to the adjusting groove 351, and a driving device for driving the adjusting rod 35 to slide along the adjusting groove 351 is disposed on the adjusting support 34. The driving device is specifically an air cylinder 36, a cylinder body of the air cylinder 36 is arranged on the adjusting support 34, an output end of the air cylinder 36 is sleeved on the adjusting rod 35, the adjusting rod 35 is driven to slide along the adjusting groove 351 by extending or contracting of the air cylinder 36, and an included angle between the fixing frame 32 and the adjusting support 34 is controlled.

As shown in fig. 3, the rocker arm stand 2 includes a base 21 and a support arm 22 with two ends hinged to each other, a magnet is arranged on the base 21 and is fixed to a desktop conveniently, a screw for adjusting tightness is arranged on a hinged point of the support arm 22, an upright column 23 is arranged on the base 21 in a rotating manner along a vertical direction, the lower end of the lower support arm is hinged to the upright column 23 along a horizontal direction, a clamp 24 is arranged at the upper end of the upper support arm and is used for clamping an injection vessel 11, the clamp 24 is detachably connected with the support arm 22, and different injection vessels can be clamped by replacing different clamps 24.

As shown in fig. 9, the metering shake-up device 4 includes a tray 41 and an electronic scale 42, the tray 41 is disposed above the working surface of the electronic scale 42 and is hinged to the electronic scale 42, and the electronic scale 42 is provided with a swing component for driving the tray 41 to swing along the hinged point.

As shown in fig. 10 and 11, the swing assembly includes a servo motor 45 and a return spring 43, the servo motor 45 and the return spring 43 are respectively disposed on two sides of a hinge point of the tray 41 and the electronic scale 42 on a working surface of the electronic scale 42, a cam 44 is disposed on an output end of the servo motor 45, an outer peripheral wall of the cam 44 is matched with a lower end surface of the tray 41, and the servo motor 45 drives the cam 44 to rotate to drive the tray 41 to swing along the hinge point. The peripheral wall of the cam 44 is provided with an arc-shaped section 441 for buffering, and the distance from the arc-shaped section 441 to the rotation center of the cam 44 is unchanged.

The working process is as follows:

in the working process, the whole system is placed in an ultra-clean workbench or a biological safety cabinet. All parts are made of stainless steel metal or corrosion-resistant plastic, and the appearance is smooth and round, so that the disinfection and safe operation are facilitated.

The priming vessel 11 uses a 50ml syringe with a piston drawn off, and by gravity, the blood or culture medium is filled via a hose into a 50ml centrifuge tube 5 placed on the metering dispensing stand 3 or into a culture bag 6 placed on the metering shake-up device 4.

The specific operation is as follows:

the operator adjusts the position height of the 50ml injector by adjusting the rocker arm bracket 2 according to the habit of the operator.

When the centrifugal tube 5 is filled, the injection needles 14 on the sub-hose 13 are clamped into the caulking grooves 321 of the fixing frame 32, the adjusting motor on the adjusting bracket 34 is matched with the air cylinder 36 on the adjusting bracket 34, and the position and the angle of the fixing frame 32 are adjusted, so that the injection needles 14 are attached to the inner wall surface of the centrifugal tube 5. Then, the operator fills the 50ml syringe with the blood cells from which the plasma is removed, the liquid is shunted along the main tube 12 through the branch tubes 13, and flows into the 50ml centrifuge tube 5 containing 20ml of the lymphocyte separation liquid, respectively, and the blood cells are stably kept above the lymphocyte separation liquid, and the separation is clear. The position of the adjusting gear 332 is adjusted through the driving motor, the adjusting gear 332 extrudes the sub-hoses 13 to different degrees, the cross section sizes of the corresponding sub-hoses 13 are controlled, and the liquid flow rate is controlled, so that the final liquid level leveling result of each centrifugal tube 5 is achieved.

When filling the culture bag 6, the main hose 12 is connected with the culture bag 6, the culture belt is placed on the tray 41, the electronic scale 42 is provided with a peeling function and a swinging frequency, the servo motor 45 connected with the tray 41 is started, the servo motor 45 drives the cam 44 to rotate, the cam 44 is matched with the return spring 43 to drive the tray 41 to swing, liquid in the culture bag 6 is uniformly shaken, after the weight of the liquid in the culture bag 6 is about to reach a specified weight, an operator stops filling the culture medium into the 50ml syringe, and liquid supplement is completed after the culture medium in the hose completely flows into the culture bag 6.

Directions and references (e.g., up, down, left, right, etc.) may be used in the present disclosure only to aid in the description of features in the figures. The following detailed description is, therefore, not to be taken in a limiting sense, and the scope of the claimed subject matter is defined only by the appended claims and equivalents thereof.

In light of the foregoing description of preferred embodiments in accordance with the invention, it is to be understood that numerous changes and modifications may be made by those skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.

Claims

1. An automatic blood and liquid dispensing system for cell culture, characterized by: include that priming device (1), rocker arm support (2), measurement formula distribution support (3) and measurement formula shake even device (4), priming device (1) is including annotating liquid household utensils (11) and main hose (12), rocker arm support (2) are used for adjusting the spatial position who annotates liquid household utensils (11), main hose (12) one end with annotate liquid household utensils (11) intercommunication, the other end is connected or is connected with cultivateing bag (6) with a plurality of branch hoses (13), it locates to cultivate bag (6) in measurement formula shakes even device (4), measurement formula shakes even device (4) and is used for shaking even and weighing the liquid of cultivateing in bag (6), be equipped with injection needle (14) on branch hose (13), be provided with a plurality of centrifuging tubes (5) in measurement formula distribution support (3), injection needle (14) support locate position and quantity on centrifuging tube (5) lateral wall with centrifuging tube (5) one-to-one, and an adjuster (33) for adjusting the section size of each branch hose (13) is arranged in the metering type distribution bracket (3).

2. An automatic blood and liquid dispensing system for cell culture according to claim 1, wherein: metering type distribution support (3) include body frame (31), it places hole (311) to be equipped with a plurality of centrifuging tubes (5) on body frame (31), it is equipped with miniature electronic scale (312) to place hole (311) bottom, it is equipped with mount (32) to place hole (311) top, mount (32) front end is equipped with caulking groove (321) with syringe needle (14) complex, mount (32) rear end is equipped with regulator (33).

3. An automatic blood and liquid dispensing system for cell culture according to claim 2, wherein: the regulator (33) comprises a frame-shaped bracket (331) and a regulating gear (332) arranged in the frame-shaped bracket (331), the two side walls of the frame-shaped bracket (331) are provided with sliding grooves (333), the sliding grooves (333) incline towards the bottom wall of the frame-shaped bracket (331) along the direction from the rear end of the frame-shaped bracket (331) to the front end of the frame-shaped bracket (331), two sides of the adjusting gear (332) are provided with connecting parts which are in sliding connection with the sliding groove (333), the sub-hose (13) is arranged between the adjusting gear (332) and the bottom wall of the frame-shaped bracket (331), the top wall of the frame-shaped bracket (331) is provided with a rack (334) engaged with the adjusting gear (332), the adjusting gear (332) is provided with a driving motor on a connecting portion, the output end of the driving motor is coaxial with the connecting portion, and the driving motor shell is attached to the outer wall surface of the frame-shaped support (331).

4. An automatic blood and liquid distribution system for cell culture according to claim 3, wherein: the bottom wall of the frame-shaped support (331) is detachably connected with the side wall of the frame-shaped support (331).

5. An automatic blood and liquid dispensing system for cell culture according to claim 2, wherein: the metering type distribution support (3) further comprises an adjusting support (34) and an adjusting rod (35), the adjusting support (34) is arranged on one side of the main frame (31), the lower end of the adjusting support (34) is hinged to the horizontal direction, an adjusting motor used for driving the adjusting support (34) to rotate along a hinged point is arranged at a hinged point of the lower end of the adjusting support (34), the lower end of the adjusting support (34) swings along the hinged point to be close to or far away from the main frame (31), the upper end of the adjusting support (34) is hinged to one side, close to the adjuster (33), of the fixing frame (32), an adjusting groove (351) is formed in the side wall of the adjusting support (34) along the length direction, one end of the adjusting rod (35) is hinged to the middle of the fixing frame (32), the other end of the adjusting rod is arranged in the adjusting groove (351) and is in sliding connection with the adjusting groove (351), and a driving device for driving the adjusting rod (35) to slide along the adjusting groove (351) is arranged on the adjusting support (34).

6. An automatic blood and liquid dispensing system for cell culture according to claim 1, wherein: rocker arm support (2) include base (21) and multistage articulated support arm (22) in proper order, be equipped with the screw of adjusting the elasticity on support arm (22) pin joint, it is provided with stand (23) to rotate along vertical direction on base (21), support arm (22) of below with stand (23) are articulated along the horizontal direction, support arm (22) of the top be used for with annotate liquid household utensils (11) and connect.

7. An automatic blood and liquid dispensing system for cell culture according to claim 6, wherein: and a clamp (24) matched with the outer wall surface of the liquid injection vessel (11) is arranged on the uppermost supporting arm (22).

8. An automatic blood and liquid dispensing system for cell culture according to claim 1, wherein: the metering type shaking device (4) comprises a tray (41) and an electronic scale (42), wherein the tray (41) is arranged above the working surface of the electronic scale (42) and hinged with the electronic scale (42), and a swinging assembly for driving the tray (41) to swing along a hinged point is arranged on the electronic scale (42).

9. An automatic blood and liquid dispensing system for cell culture according to claim 8, wherein: the swing assembly comprises a servo motor (45) and a reset spring (43), the servo motor (45) and the reset spring (43) are arranged on the working surface of the electronic scale (42) and are respectively arranged on two sides of a hinge point of the tray (41) and the electronic scale (42), a cam (44) is arranged on the output end of the servo motor (45), the peripheral wall of the cam (44) is matched with the lower end surface of the tray (41), and the servo motor (45) drives the cam (44) to rotate and drive the tray (41) to swing along the hinge point.

10. An automatic blood and liquid dispensing system for cell culture according to claim 9, wherein: the buffer cam is characterized in that an arc-shaped section (441) for buffering is arranged on the outer peripheral wall of the cam (44), and the distance from the arc-shaped section (441) to the rotation center of the cam (44) is unchanged.