CN114224935A - Cold compress spray for relieving gout and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a cold compress spray for relieving gout and a preparation method thereof; the cold compress spray for relieving gout comprises: the cold compress spray is an external medicine, adverse reaction and toxic and side effects are remarkably reduced, and the cold compress spray has good permeability, can immediately decompose the tophus after being quickly kneaded and absorbed by hands, promotes blood circulation, quickly relieves pain, has obvious treatment effect, remarkably improves the medication compliance of patients with gouty arthritis, and improves the life quality of the patients.

Description

Cold compress spray for relieving gout and preparation method thereof

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a cold compress spray for relieving gout and a preparation method thereof.

Background

Gout is directly related to hyperuricemia caused by purine metabolic disorder and/or reduction of uric acid excretion, and belongs to the category of metabolic rheumatism. Gout can be complicated with kidney disease, and severe cases can cause joint destruction and renal function damage, often accompanied with hyperlipidemia, hypertension, diabetes, arteriosclerosis, coronary heart disease, etc. Hyperuricemia is the basis for the development of gout.

Gout belongs to the categories of arthralgia syndrome, gout, historical segment and the like in traditional Chinese medicine, and refers to symptoms of arthralgia, myalgia, numbness, heaviness and unsmooth flexion and extension caused by exogenous pathogenic factors blocking channels and collaterals, stagnation of qi and blood and deficiency of liver and kidney. Gout patients often develop tophi. Seen subcutaneously around the pinna, around the joints, tendons, soft tissues, etc. of the patient. Tophus formed on various parts of the body, especially four limbs, not only seriously affects the appearance of the limbs, but also leads to joint deformity, dysfunction, nerve compression, skin ulceration and prolonged unhealed sinus, and needs to be treated by operation.

At present, colchicine is used as a treatment medicament for gouty arthritis, and Baotan or indomethacin can be selected. The continuous rise of serum uric acid can also be treated by using the carbendazim, and the medicine plays a role in treating the reabsorption of urate by inhibiting renal tubules and is one of effective treatment medicines. If kidney disease is present, allopurines are often the treatment of choice. In the acute stage, steroid injection into joints, joint immobilization and cold compress of local parts can obviously relieve symptoms. However, although the western medicines can rapidly relieve symptoms, the recurrence rate is high, and the disease can rebound after the medicine is stopped, the western medicines must be taken for a long time without interruption, so that the western medicines bring a large economic burden to patients, and the western medicines have large toxic and side effects on the kidney after long-term administration. Therefore, the development of a spray for effectively treating gouty arthritis is a problem which needs to be solved at present.

Disclosure of Invention

The invention aims to provide a cold compress spray for relieving gout, which has the advantages of quick response, short treatment course, low toxic and side effects, simple preparation process and convenient use.

Specifically, the present invention provides:

a cold compress spray for relieving gout comprises: alkaline ionized water, sodium bicarbonate, L-carnitine, eucommia bark water extract, green olive juice and monosodium glutamate.

The cold compress spray for relieving gout comprises the following components in parts by weight:

the pH value of the alkaline ionized water is 10.0-10.9.

A preparation method of a cold compress spray for relieving gout comprises the following steps:

step 1, adding baking soda into alkaline ionized water, and after uniformly stirring, enabling the pH value to reach 12-13;

step 2, adding L-carnitine into the soda alkaline water obtained in the step 1, uniformly stirring, and standing;

step 3, adding the green olive juice of the eucommia bark water extract into the solution obtained in the step 2, and filtering;

step 4, heating the filtrate obtained in the step 3, adding monosodium glutamate, standing and filtering;

and 5, concentrating the liquid obtained in the step 4 to a relative density of 1.10-1.15 at 25 ℃, and filling the spray into a filling machine to obtain the cold compress spray.

The alkaline ionized water of the present invention is water that is directly collected or produced by an alkaline ion water treatment apparatus (also referred to as an alkaline ionizer) and exhibits alkalinity, and is also referred to as electrolyzed water. The alkaline ionized water is purchased from the Kyoto Shanghai city, and the brand is imported table salt ocean alkaline ionized water.

Preparing a eucommia bark water extract: pulverizing cortex Eucommiae, sieving with 60 mesh sieve, weighing 40g powder, boiling in 1000mL sterile distilled water for 4h, filtering with 300 mesh sieve, collecting filtrate, concentrating in water bath, evaporating to obtain dry liquid, and adding distilled water to obtain cortex Eucommiae water extract with concentration of 3 g/L.

Preparation of green olive juice: putting the cleaned green olive into boiling water, adding 0.1% citric acid solution, scalding for 5min, taking out, washing in flowing water to remove surface wax, softening pulp tissue, and protecting color; extracting the pitted olive pulp in a material-water ratio of 1:5(g/mL) for 2h at 50 ℃ and 400r/min in a temperature-controlled stirrer; filtering the extracted olive juice with gauze to obtain clear and transparent green olive juice.

The baking soda and the sodium bicarbonate have the molecular formulaNaHCO₃Is an inorganic salt. Purchased from the market.

The L-carnitine, also called L-carnitine and vitamin BT, has a chemical formula of C₇H₁₅NO₃Purchased from the market.

The monosodium glutamate is sodium glutamate, is a delicate flavor seasoning, and is easily soluble in water; purchased from the market.

Compared with the prior art, the invention has the following advantages and positive effects:

1. the spray is an external medicine, adverse reactions and toxic and side effects are remarkably reduced, the permeability of the spray is good, and after the spray is quickly kneaded and absorbed by hands, the tophus can be immediately decomposed to promote blood circulation, so that pains are quickly relieved, the treatment effect is obvious, the medication compliance of patients with gouty arthritis is remarkably improved, and the life quality of the patients is improved;

2. the invention has scientific compatibility, has the advantages of quick response, short treatment course, low cost and the like, and has simple and convenient preparation process, stable and safe drug effect, stable preparation system, long-term storage, convenient use and easy popularization and application.

Detailed Description

The present invention is further described in the following description of the specific embodiments, which is not intended to limit the invention, but various modifications and improvements can be made by those skilled in the art according to the basic idea of the invention, within the scope of the invention, as long as they do not depart from the basic idea of the invention.

Preparing a eucommia bark water extract: pulverizing cortex Eucommiae, sieving with 60 mesh sieve, weighing 40g powder, boiling in 1000mL sterile distilled water for 4h, filtering with 300 mesh sieve, collecting filtrate, concentrating in water bath, evaporating to obtain dry liquid, and adding distilled water to obtain cortex Eucommiae water extract with concentration of 3 g/L.

Preparation of green olive juice: putting the cleaned green olive into boiling water, adding 0.1% citric acid solution, scalding for 5min, taking out, washing in flowing water to remove surface wax, softening pulp tissue, and protecting color; extracting the pitted olive pulp in a material-water ratio of 1:5(g/mL) for 2h at 50 ℃ and 400r/min in a temperature-controlled stirrer; filtering the extracted olive juice with gauze to obtain clear and transparent green olive juice.

The baking soda and the sodium bicarbonate have the molecular formula of NaHCO₃Is an inorganic salt. Purchased from the market.

The L-carnitine disclosed by the invention is also called L-carnitine and vitamin BT, has a chemical formula of C7H15NO3, and is purchased from the market.

The monosodium glutamate is sodium glutamate, is a delicate flavor seasoning, and is easily soluble in water; purchased from the market.

Example 1

Step 1, adding 0.2g of baking soda into 99ml of alkaline ionized water, and uniformly stirring until the pH value reaches 12.5;

step 2, adding 0.1g of L-carnitine into the soda alkaline water obtained in the step 1, uniformly stirring, and standing;

step 3, adding 0.1g of eucommia bark water extract and 0.01g of green olive juice into the solution obtained in the step 2, and filtering;

step 4, heating the filtrate obtained in the step 3, adding 0.01g of monosodium glutamate, standing and filtering;

and 5, concentrating the liquid obtained in the step 4 to a relative density of 1.10-1.15 at 25 ℃, and filling the spray into a filling machine to obtain the cold compress spray.

Example 2

Step 1, adding 1.8 of baking soda into 97g of alkaline ionized water, and uniformly stirring until the pH value reaches 13;

step 2, adding 0.5g of L-carnitine into the soda alkaline water obtained in the step 1, uniformly stirring, and standing;

step 3, adding 0.5g of eucommia bark water extract and 0.1g of green olive juice into the solution obtained in the step 2, and filtering;

step 4, heating the filtrate obtained in the step 3, adding 0.1g of monosodium glutamate, standing and filtering;

and 5, concentrating the liquid obtained in the step 4 to a relative density of 1.10-1.15 at 25 ℃, and filling the spray into a filling machine to obtain the cold compress spray.

Example 3

Step 1, adding 1.0g of baking soda into 98g of alkaline ionized water, and uniformly stirring until the pH value reaches 12-13;

step 2, adding 0.2g of L-carnitine into the soda alkaline water obtained in the step 1, uniformly stirring, and standing;

step 3, adding 0.4g of eucommia bark water extract and 0.04g of green olive juice into the solution obtained in the step 2, and filtering;

step 4, heating the filtrate obtained in the step 3, adding 0.06g of monosodium glutamate, standing and filtering;

and 5, concentrating the liquid obtained in the step 4 to a relative density of 1.10-1.15 at 25 ℃, and filling the spray into a filling machine to obtain the cold compress spray.

Comparative example 1:

step 1, adding 1.0g of baking soda into 98g of deionized water, and uniformly stirring;

step 2, adding 0.2g of L-carnitine into the soda alkaline water obtained in the step 1, uniformly stirring, and standing;

step 3, adding 0.04g of green olive juice into the solution obtained in the step 2, and filtering;

step 4, heating the filtrate obtained in the step 3, adding 0.06g of monosodium glutamate, standing and filtering;

and 5, concentrating the liquid obtained in the step 4 to a relative density of 1.10-1.15 at 25 ℃, and filling the spray into a filling machine to obtain the cold compress spray. .

Comparative example 2

Step 1, adding 1.0g of baking soda into 98g of alkaline ionized water, and uniformly stirring until the pH value reaches 12-13;

step 2, adding 0.4g of eucommia bark water extract into the solution obtained in the step 1, and filtering;

step 3, heating the filtrate obtained in the step 2, adding 0.06g of monosodium glutamate, standing and filtering;

and 4, concentrating the liquid obtained in the step 3 to a relative density of 1.10-1.15 at 25 ℃, and filling the spray into a filling machine to obtain the cold compress spray.

The first experimental example: acute footpad swelling test of sodium urate crystal of spray rat

Preparing sodium urate crystal and sodium urate solution: adding 2g of uric acid into 400ml of boiling water, adjusting the pH value to 7.4 by using NaOH (1N), heating to 95 ℃, cooling at room temperature, stirring lightly, standing at 4 ℃ for 4-6 hours, separating out flocculent precipitate, filtering to obtain microcrystalline sodium urate, and sterilizing at 80 ℃. 1000mg of sodium urate is weighed before use, fully ground and added with 20ml of sterile physiological saline to prepare 50g/L (5%) of suspension.

Grouping and administration: rats were fed adaptively for 3 days, and were randomly divided into 5 groups by weight: model control group, positive drug indomethacin group (0.0045g/kg), experiment 1 group, experiment 2 group, and experiment 3 group. The indometacin group as the positive drug is administered by intragastric administration (10ml/kg) 1 hour before causing inflammation; the other groups were coated immediately after molding (0.1L/piece), and were re-coated 6, 12, and 24 hours after inflammation, and the model groups were coated with the same volume of vehicle.

Experiment 1 group sprays prepared in example 1 of the present invention.

Experiment 2 group sprays prepared in example 2 of the present invention.

Experiment 3 group sprays prepared in example 3 of the present invention.

Comparative example 1 group.

Comparative example 2 group.

Preparing a model: the circumference of the joint was measured as a normal value by a plantar volume measuring instrument. In each group, 0.2mL of 5% sodium urate suspension was injected into the dorsal aspect of the right ankle joint (joint extension, 45 degree needle insertion) of the rats for joint cavity molding.

Swelling degree of joints: the volume of the right ankle joint was measured 6, 12, 24, 48 hours after the onset of inflammation. And the swelling degree of the joint was calculated, and the results are shown in table 1.

Swelling degree is equal to the volume of the joint after molding-the volume of the joint before molding

Table 1 effect on sodium urate induced ankle swelling in rats (X ± SD, n ═ 9)

And (4) conclusion: modeling comparison group: after 6h, the ankle joint volume of the rat is obviously increased, swelling is gradually reduced along with the time, but the ankle joint does not return to normal after 48h, and the difference is significant compared with that before inflammation (P is less than 0.01). Compared with the model group, the experiment 1 group, the experiment 2 group and the experiment 3 group obviously reduce the ankle joint volume of the rat (P is less than 0.05 and P is less than 0.01); while the comparative example 1 and comparative example 2 did not improve ankle joint volume, and were not statistically significant compared to the model control group.

Experimental example II analgesic test of spray of the present invention

A hot plate method is adopted, a mouse with the weight of 20-24 g and a female are taken, a physio-physiological experiment multi-purpose instrument is used, the temperature is adjusted to be 55 +/-0.5 ℃, and the foot of the mouse licked is used as an index of pain. The pain threshold was determined once every 5min for 2 times before administration and the mean was taken. Selecting mice with pain threshold value of 10-30 seconds, stable reaction and no jumping for test, wherein each group comprises 10 mice and is randomly divided into the following groups:

matrix group, 70% ethanol solution is given;

experiment 1 group sprays prepared in example 1 of the present invention;

experiment 2 group spray prepared in example 2 of the present invention;

experiment 3 group sprays made in example 3 of the present invention.

Comparative example 1 group.

Comparative example 2 group.

Each group of the double-sided hind paw primers was 15. mu.l/body, 15 min/time X3 times. The pain threshold is re-measured 15min, 30 min and 60 min after the last application, and is measured twice at 5min intervals, and the average is taken as the pain threshold. The results are shown in Table 2.

TABLE 2 Effect of sprays of the invention on Hot plate pain threshold in mice

The results prove that: the local spraying agent can improve the pain threshold caused by mouse hot plate method; while the sprays of the comparative examples did not increase the pain threshold in mice, it is sufficient to show that the composition of the invention is not a simple combination but an organic combination in its entirety.

Claims (4)

1. A cold compress spray for relieving gout is characterized in that the spray comprises the following raw materials: alkaline ionized water, sodium bicarbonate, L-carnitine, eucommia bark water extract, green olive juice and monosodium glutamate.

2. The cold compress spray for relieving gout as claimed in claim 1, which is characterized by comprising the following components in parts by weight:

3. the cold compress spray for relieving gout as claimed in claim 1, wherein the pH value of the alkaline ionized water is 10.0-10.9.

4. The cold compress spray for relieving gout as claimed in claim 1, wherein the preparation method of the spray comprises the following steps:

step 1, adding baking soda into alkaline ionized water, and after uniformly stirring, enabling the pH value to reach 12-13;

step 2, adding L-carnitine into the soda alkaline water obtained in the step 1, uniformly stirring, and standing;

step 3, adding the eucommia bark water extract and the green olive juice into the solution obtained in the step 2, and filtering;

step 4, heating the filtrate obtained in the step 3, adding monosodium glutamate, standing and filtering;

and 5, concentrating the liquid obtained in the step 4 to a relative density of 1.10-1.15 at 25 ℃, and filling the spray into a filling machine to obtain the cold compress spray.