CN113951122A - Cultivation method for improving survival rate of curcuma kwangsiensis tissue culture seedlings - Google Patents
Cultivation method for improving survival rate of curcuma kwangsiensis tissue culture seedlings Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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Abstract
The invention discloses a cultivation method for improving survival rate of curcuma zedoaria tissue culture seedlings, which comprises the following steps: culturing the curcuma kwangsiensis tissue culture seedlings in a matrix, wherein the matrix at least comprises a first matrix layer formed by a first matrix, the first matrix is composed of river sand, sawdust, peat and perlite, and the mass ratio of the river sand, the sawdust, the peat and the perlite is 1-3: 2-6: 1-3. The improved substrate is adopted to improve the survival rate of transplanting the tissue culture seedlings of curcuma kwangsiensis, promote the growth of plants and shorten the growth period of curcuma kwangsiensis.
Description
Technical Field
The invention relates to the technical field of rapid propagation of plant tissue culture seedlings, in particular to a cultivation method for improving the survival rate of curcuma zedoary tissue culture seedlings.
Background
Curcuma kwangsiensis S.G.Lee et C.F.Liang in Guangxi is a perennial herb of Curcuma in Zingiberaceae, the rhizome and root tuber are one of the traditional Chinese medicinal materials of rhizoma Curcumae and radix Curcumae primordium specified in the 'Chinese pharmacopoeia' of 2020 edition, and has the effects of promoting blood circulation, relieving pain, promoting qi circulation, relieving depression, clearing heart fire, cooling blood, benefiting gallbladder, removing jaundice and the like, and has the efficacies of mainly treating epilepsy, mania, stabbing pain in chest and hypochondrium, chest stuffiness, cardialgia, amenorrhea and dysmenorrheal. In recent years, curcuma kwangsiensis is widely applied clinically, the demand of curcuma kwangsiensis is increasing, and planting of curcuma kwangsiensis becomes a new industry for gathering of agriculture and farmers with Guangxi characteristics. The Guangxi zedoary is mainly bred by rhizomes in production, has the problems of large seed consumption and low breeding speed, and can realize large-scale production of the Guangxi zedoary in a short time by adopting a plant tissue culture technology. However, the problems of low survival rate, slow growth and the like of the tissue culture seedlings exist after the tissue culture seedlings of curcuma kwangsiensis are rooted and transplanted, and the development of the tissue culture seedling-culturing and rapid propagation technology of curcuma kwangsiensis is restricted.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
One object of the present invention is to provide a method for culturing curcuma zedoary, which can increase the survival rate of curcuma zedoary tissue culture seedlings in Guangxi, promote the growth of plants, and shorten the growth cycle of curcuma zedoary in Guangxi.
To achieve these objects and other advantages in accordance with the present invention, there is provided a cultivation method for increasing survival rate of curcuma zedoaria tissue culture seedlings, comprising:
culturing the curcuma kwangsiensis tissue culture seedlings in a matrix, wherein the matrix at least comprises a first matrix layer formed by a first matrix, the first matrix is composed of river sand, sawdust, peat and perlite, and the mass ratio of the river sand, the sawdust, the peat and the perlite is 1-3: 2-6: 1-3.
Preferably, in the cultivation method for improving the survival rate of the curcuma zedoary tissue culture seedlings in Guangxi, the mass ratio of the river sand, the wood chips, the peat and the perlite in the first substrate is 1:1:2: 1.
Preferably, in the cultivation method for improving survival rate of curcuma kwangsiensis tissue culture seedlings, before the curcuma kwangsiensis tissue culture seedlings are cultivated in the matrix, the method further comprises:
and (3) carrying out moist heat sterilization on the river sand in the first matrix at the temperature of 120 ℃ for 25 minutes.
Preferably, in the cultivation method for improving the survival rate of the curcuma zedoary tissue culture seedlings in Guangxi, the first matrix is an unused new matrix; before the curcuma kwangsiensis tissue culture seedlings are cultivated in the matrix, the method further comprises the following steps:
carrying out recovery fermentation treatment on the used first substrate to obtain a second substrate;
laying the second substrate to form a second substrate layer;
laying the first substrate above the second substrate layer to form a first substrate layer, wherein the thickness of the second substrate layer is one third of the thickness of the first substrate layer.
Preferably, in the cultivation method for improving survival rate of curcuma zedoaria tissue culture seedlings in Guangxi, the step of recovering and fermenting the used first substrate to obtain the second substrate comprises the following steps:
cutting Guangxi rhizoma zedoariae waste materials, wherein the Guangxi rhizoma zedoariae waste materials comprise stem leaves and root hair parts left by the part of the Guangxi rhizoma zedoariae which is extracted with medicine, and/or the whole plant of the Guangxi rhizoma zedoariae which is in bad growth state;
mixing the Guangxi curcuma zedoary waste into the used first matrix to obtain a mixed material, controlling the water content of the mixed material not to exceed 10%, wherein the mass ratio of the Guangxi curcuma zedoary waste to the used first matrix is 1: 3-4;
and mixing EM bacteria in the mixed material, stacking and fermenting for 20-40 days, turning over once every 10 days in the fermentation process, and obtaining a second substrate after the fermentation is finished.
Preferably, in the cultivation method for improving the survival rate of the curcuma kwangsiensis tissue culture seedlings, the mass ratio of the curcuma kwangsiensis waste to the used first matrix is 1: 4.
Preferably, in the cultivation method for improving the survival rate of the curcuma zedoary tissue culture seedlings in Guangxi, EM bacteria are mixed in the mixed materials, and stacking fermentation is carried out for 40 days.
The invention at least comprises the following beneficial effects:
the zedoary cultivation method comprises the following steps: culturing the curcuma kwangsiensis tissue culture seedlings in a matrix, wherein the matrix at least comprises a first matrix layer formed by a first matrix, the first matrix is composed of river sand, sawdust, peat and perlite, and the mass ratio of the river sand, the sawdust, the peat and the perlite is 1-3: 2-6: 1-3. The improved substrate is adopted to improve the survival rate of transplanting the tissue culture seedlings of curcuma kwangsiensis, promote the growth of plants and shorten the growth period of curcuma kwangsiensis.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a photograph showing the growth of a transplanted seedling of Curcuma kwangsiensis in example 1 of the present invention.
Detailed Description
The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
The invention provides a cultivation method for improving survival rate of curcuma zedoaria tissue culture seedlings, which comprises the following steps: culturing the curcuma kwangsiensis tissue culture seedlings in a matrix, wherein the matrix at least comprises a first matrix layer formed by a first matrix, the first matrix is composed of river sand, sawdust, peat and perlite, and the mass ratio of the river sand, the sawdust, the peat and the perlite is 1-3: 2-6: 1-3. The first matrix consisting of river sand, sawdust, peat and perlite is loose and fertile, has good drainage and can meet the growth requirement of tissue culture seedlings. Based on the method, the survival rate of transplanting the tissue culture seedlings of the curcuma kwangsiensis is improved by adopting the improved matrix, the plant growth is promoted, and the growth cycle of the curcuma kwangsiensis is shortened.
In some preferred embodiments, in the cultivation method for improving survival rate of curcuma zedoary tissue culture seedlings, in the first medium, the mass ratio of the river sand, the wood chips, the peat and the perlite is 1:1:2: 1.
In some preferred embodiments, in the cultivation method for improving survival rate of curcuma kwangsiensis tissue culture seedlings, before the cultivation of the curcuma kwangsiensis tissue culture seedlings in the medium, the method further comprises: and (3) carrying out moist heat sterilization on the river sand in the first matrix at the temperature of 120 ℃ for 25 minutes. Here, the river sand is sterilized under certain conditions to avoid pest and disease damage of the tissue culture seedlings.
In some preferred embodiments, in the cultivation method for improving survival rate of curcuma zedoaria tissue culture seedlings, the first substrate is an unused new substrate; before the curcuma kwangsiensis tissue culture seedlings are cultivated in the matrix, the method further comprises the following steps: carrying out recovery fermentation treatment on the used first substrate to obtain a second substrate; laying the second substrate to form a second substrate layer; laying the first substrate above the second substrate layer to form a first substrate layer, wherein the thickness of the second substrate layer is one third of the thickness of the first substrate layer.
In order to improve the growth state of the tissue culture seedling and promote the growth of the plant, a new unused first substrate can be adopted each time the tissue culture seedling is transplanted from the culture bottle to the substrate. However, this practice results in a certain degree of waste, resulting in an increase in the cost of cultivation. Therefore, the used first substrate is recovered and is used as a second substrate after fermentation treatment and is laid below the first substrate layer. The first substrate is a new unused substrate, the first substrate is loose and fertile, the drainage is good, the second substrate obtained by fermentation treatment can kill germs, and the second substrate is compact in structure and richer in nutrition. The use of two layers of substrates can meet the growth of tissue culture seedlings. Based on the matrix setting mode, the use of new matrix can be saved, the cost is saved, the threat of plant diseases and insect pests possibly caused by the repeated use of the matrix can be avoided, the structure of the matrix can be improved, the upper layer of the matrix is loose, the lower layer of the matrix is compact, the nutrition is rich, and the growth of plants is facilitated.
Specifically, in the cultivation method for improving survival rate of curcuma zedoary tissue culture seedlings in Guangxi, the used first substrate is recycled and fermented to obtain a second substrate, and the cultivation method comprises the following steps: cutting Guangxi rhizoma zedoariae waste materials, wherein the Guangxi rhizoma zedoariae waste materials comprise stem leaves and root hair parts left by the part of the Guangxi rhizoma zedoariae which is extracted with medicine, and/or the whole plant of the Guangxi rhizoma zedoariae which is in bad growth state; mixing the Guangxi curcuma zedoary waste into the used first matrix to obtain a mixed material, controlling the water content of the mixed material not to exceed 10%, wherein the mass ratio of the Guangxi curcuma zedoary waste to the used first matrix is 1: 3-4; and mixing EM bacteria in the mixed material, stacking and fermenting for 20-40 days, turning over once every 10 days in the fermentation process, and obtaining a second substrate after the fermentation is finished.
When in collection, the stem and leaf parts of the curcuma kwangsiensis are discarded and the root and hair parts are also removed after drying. In addition, some plants with poor growth state can be directly discarded. These waste fractions still contain small amounts of nutrients, which are needed for plant growth. Therefore, these waste portions are recovered, minced, and then fermented together with the recovered first substrate, and the second substrate layer is laid as a second substrate after fermentation. The curcuma kwangsiensis waste is mixed with the used first substrate, and is stacked and fermented by EM (effective microorganisms) to kill germs in the substrate, increase nutrient substances in the second substrate and utilize plant growth. Through the fermentation process, the purpose of reutilization of the used first matrix can be achieved, waste is reduced, the plant growth requirement can be met, the survival rate of transplanting of the curcuma kwangsiensis tissue culture seedlings is improved, the plant growth is promoted, and the growth period of the curcuma kwangsiensis is shortened.
In some preferred embodiments, in the cultivation method for improving survival rate of curcuma kwangsiensis tissue culture seedlings, the mass ratio of the curcuma kwangsiensis waste to the used first matrix is 1: 4.
In some preferred embodiments, in the cultivation method for improving the survival rate of the curcuma zedoary tissue culture seedlings, EM bacteria are mixed in the mixed material, and the mixture is stacked and fermented for 40 days.
Example 1
In this example, curcuma zedoaria was collected in the area of southwestern Guangxi for tissue culture in southwestern Guangxi, 200 bottles of rooting tissue culture seedlings with root length of 2-5 cm were selected, 10 seedlings per bottle were used on average for greenhouse transplantation test.
The method comprises the following specific operation steps: and cleaning the tissue culture seedlings by clear water, draining water and naturally drying. Planting the tissue culture seedlings in a matrix, wherein the mass ratio of river sand, wood chips, peat and perlite in the matrix is 1:1:2:1, placing the matrix in a greenhouse for cultivation, and cultivating the matrix under the conditions that the illumination intensity is 6000Lx, the temperature is 30 ℃ and the humidity is 60%. After 1 month of growth, the survival rate of the tissue culture seedlings after being taken out of the bottles and the growth condition of transplantation are observed.
FIG. 1 shows the growth of Curcuma kwangsiensis tissue culture seedlings transplanted in a matrix for 1 month. As can be seen from figure 1, the Curcuma kwangsiensis tissue culture seedlings have good growth state after being transplanted in a matrix and grown for 1 month, and the plant height reaches 8-12 cm. And moreover, the survival rate of the tissue culture seedlings transplanted in the matrix is counted, the survival rate of the tissue culture seedlings of curcuma kwangxi planted in a Nanning greenhouse base is 100%, and the plant height is 8-12 cm.
Example 2
In this example, curcuma kwangsiensis cultured seedlings (induced to root) were selected for greenhouse transplantation test. And (3) washing the tissue culture seedlings induced to root off the culture medium by using clear water, wherein the plant height is 1.5-3 cm, and airing at room temperature.
3 kinds of matrixes were selected. The matrix A only consists of wood chips and peat, and the mass ratio of the wood chips to the peat is 1: 1; the matrix B is composed of river sand, sawdust and peat, and the mass ratio of the river sand to the sawdust to the peat is 1:1: 1; the medium C is composed of river sand, wood chips, peat and perlite, wherein the mass ratio of the river sand to the wood chips to the peat to the perlite is 1:1:2: 1.
After planting the tissue culture seedlings on different substrates, placing the tissue culture seedlings in greenhouses at 24 ℃, 30 ℃ and 37 ℃, adjusting the illumination of the greenhouses at different temperatures to 3 illumination gradients (6000LX, 3000LX and 500LX), and culturing for 30 days to count the survival rate and the plant height (see table 1).
TABLE 3 growth of Curcuma kwangsiensis in different media
As can be seen from Table 1, the survival rate of the tissue culture seedling is high and the growth vigor is good only under the condition of the medium C containing 4 components such as river sand, wood chips, peat, perlite and the like, and the plant height can reach 6-8 cm or 8-12 cm.
Example 3
Preparing a matrix:
(1) and (3) carrying out recovery fermentation treatment on the used first substrate to obtain a second substrate.
a) Cutting the Guangxi zedoary waste materials, wherein the Guangxi zedoary waste materials comprise stem leaves and root hair parts left by the collected medicinal parts of the Guangxi zedoary, and/or the whole Guangxi zedoary plant with poor growth state, and the like.
b) Mixing Guangxi curcuma zedoary waste into the used first matrix to obtain a mixed material, controlling the water content of the mixed material not to exceed 10%, wherein the mass ratio of the Guangxi curcuma zedoary waste to the used first matrix is 1: 4.
c) Mixing EM bacteria in the mixed material, stacking and fermenting for 20 days, turning over once every 10 days in the fermentation process, and obtaining a second substrate after the fermentation is finished.
(2) Laying a second substrate to form a second substrate layer, the second substrate layer having a thickness of 1 cm.
(3) And laying the first substrate above the second substrate layer to form the first substrate layer, wherein the thickness of the second substrate layer is one third of that of the first substrate layer.
In this example, curcuma zedoaria was collected in the area of southwestern Guangxi for tissue culture in southwestern Guangxi, 200 bottles of rooting tissue culture seedlings with root length of 2-5 cm were selected, 10 seedlings per bottle were used on average for greenhouse transplantation test.
The method comprises the following specific operation steps: and cleaning the tissue culture seedlings by clear water, draining water and naturally drying. Planting the tissue culture seedlings in a matrix, placing the matrix in a greenhouse for cultivation, and cultivating the tissue culture seedlings under the conditions that the illumination intensity is 6000Lx, the temperature is 30 ℃ and the humidity is 60%. After 1 month of growth, the survival rate of the tissue culture seedlings after being taken out of the bottles and the growth condition of transplantation are counted. The survival rate of the tissue culture seedlings can reach 100%, the growth vigor is good, and the plant height can reach 12-15 cm.
Example 4
Preparing a matrix:
(1) and (3) carrying out recovery fermentation treatment on the used first substrate to obtain a second substrate.
a) Cutting the Guangxi zedoary waste materials, wherein the Guangxi zedoary waste materials comprise stem leaves and root hair parts left by the collected medicinal parts of the Guangxi zedoary, and/or the whole Guangxi zedoary plant with poor growth state, and the like.
b) Mixing Guangxi curcuma zedoary waste into the used first matrix to obtain a mixed material, controlling the water content of the mixed material not to exceed 10%, wherein the mass ratio of the Guangxi curcuma zedoary waste to the used first matrix is 1: 3.
c) Mixing EM bacteria in the mixed material, stacking and fermenting for 40 days, turning over once every 10 days in the fermentation process, and obtaining a second substrate after the fermentation is finished.
(2) Laying a second substrate to form a second substrate layer, the second substrate layer having a thickness of 1.5 cm.
(3) And laying the first substrate above the second substrate layer to form the first substrate layer, wherein the thickness of the second substrate layer is one third of that of the first substrate layer.
In this example, curcuma zedoaria was collected in the area of southwestern Guangxi for tissue culture in southwestern Guangxi, 200 bottles of rooting tissue culture seedlings with root length of 2-5 cm were selected, 10 seedlings per bottle were used on average for greenhouse transplantation test.
The method comprises the following specific operation steps: and cleaning the tissue culture seedlings by clear water, draining water and naturally drying. Planting the tissue culture seedlings in a matrix, placing the matrix in a greenhouse for cultivation, and cultivating the tissue culture seedlings under the conditions that the illumination intensity is 6000Lx, the temperature is 30 ℃ and the humidity is 60%. After 1 month of growth, the survival rate of the tissue culture seedlings after being taken out of the bottles and the growth condition of transplantation are counted. The survival rate of the tissue culture seedlings can reach 100%, the growth vigor is good, and the plant height can reach 12-15 cm.
In conclusion, the survival rate of transplanted curcuma zedoary tissue culture seedlings is improved based on the improved matrix, the plant growth is promoted, and the growth cycle of curcuma zedoary is shortened. The method promotes the large-scale cultivation and the marketization application of the curcuma zedoary through the perfect efficient breeding matrix of the curcuma zedoary, solves the problems of low survival rate, slow growth and the like of the curcuma zedoary tissue culture seedlings, has good practicability, and can bring good social and economic benefits.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the uses set forth in the specification and examples. It can be applied to all kinds of fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. It is therefore intended that the invention not be limited to the exact details and illustrations described and illustrated herein, but fall within the scope of the appended claims and equivalents thereof.
Claims (7)
1. A cultivation method for improving survival rate of Curcuma kwangsiensis tissue culture seedlings is characterized by comprising the following steps:
culturing the curcuma kwangsiensis tissue culture seedlings in a matrix, wherein the matrix at least comprises a first matrix layer formed by a first matrix, the first matrix is composed of river sand, sawdust, peat and perlite, and the mass ratio of the river sand, the sawdust, the peat and the perlite is 1-3: 2-6: 1-3.
2. The cultivation method for improving the survival rate of zedoary turmeric tissue culture seedlings as claimed in claim 1, wherein the mass ratio of said river sand, said wood chips, said peat and said perlite in said first medium is 1:1:2: 1.
3. The cultivation method for improving the survival rate of curcuma kwangsiensis tissue culture seedlings according to claim 1, wherein before the cultivation of curcuma kwangsiensis tissue culture seedlings in the medium, the method further comprises:
and (3) carrying out moist heat sterilization on the river sand in the first matrix at the temperature of 120 ℃ for 25 minutes.
4. The cultivation method for improving the survival rate of zedoary turmeric tissue culture seedlings as claimed in claim 1, wherein said first substrate is a new unused substrate; before the curcuma kwangsiensis tissue culture seedlings are cultivated in the matrix, the method further comprises the following steps:
carrying out recovery fermentation treatment on the used first substrate to obtain a second substrate;
laying the second substrate to form a second substrate layer;
laying the first substrate above the second substrate layer to form a first substrate layer, wherein the thickness of the second substrate layer is one third of the thickness of the first substrate layer.
5. The cultivation method for improving the survival rate of zedoary turmeric tissue culture seedlings as claimed in claim 4, wherein said recovering and fermenting the used first substrate to obtain the second substrate comprises:
cutting Guangxi rhizoma zedoariae waste materials, wherein the Guangxi rhizoma zedoariae waste materials comprise stem leaves and root hair parts left by the part of the Guangxi rhizoma zedoariae which is extracted with medicine, and/or the whole plant of the Guangxi rhizoma zedoariae which is in bad growth state;
mixing the Guangxi curcuma zedoary waste into the used first matrix to obtain a mixed material, controlling the water content of the mixed material not to exceed 10%, wherein the mass ratio of the Guangxi curcuma zedoary waste to the used first matrix is 1: 3-4;
and mixing EM bacteria in the mixed material, stacking and fermenting for 20-40 days, turning over once every 10 days in the fermentation process, and obtaining a second substrate after the fermentation is finished.
6. The cultivation method for improving the survival rate of the curcuma kwangsiensis tissue culture seedlings according to claim 5, wherein the mass ratio of the curcuma kwangsiensis waste to the used first matrix is 1: 4.
7. The cultivation method for improving the survival rate of zedoary turmeric tissue culture seedlings as claimed in claim 5, wherein EM bacteria is mixed in said mixture, and the mixture is piled and fermented for 40 days.
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