CN113866444B - An automatic sample adding and mixing device for spectrophotometry pretreatment - Google Patents
An automatic sample adding and mixing device for spectrophotometry pretreatmentInfo
- Publication number
- CN113866444B CN113866444B CN202111121101.0A CN202111121101A CN113866444B CN 113866444 B CN113866444 B CN 113866444B CN 202111121101 A CN202111121101 A CN 202111121101A CN 113866444 B CN113866444 B CN 113866444B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00376—Conductive heating, e.g. heated plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides an automatic sample adding and mixing device for spectrophotometry pretreatment, which mainly comprises a base, a sample adding and sampling device for adding a buffering agent and sampling a sample cup after adding the buffering agent and mixing uniformly, a transmission and mixing device for transmitting the sample cup and a cuvette, a weighing device for weighing the whole weight of the sample cup, a reagent adding device for adding experimental reagents, a cleaning device for cleaning a sample adding and sampling needle and a reagent needle, a control system for controlling the devices and a control terminal, wherein the control terminal mainly exists in a network computer and runs control software. According to the invention, the sample adding and sampling device, the transmission mixing device, the weighing device, the reagent adding device, the cleaning device, the control system and the control terminal are matched for use, so that the automatic sample adding of the pretreatment part cuvette in a spectrophotometry is realized, the efficiency and the accuracy are high, the error is small, the cost of manual sample adding of professional experimenters is reduced, and the cost is saved.
Description
Technical Field
The invention mainly relates to the technical field of detection equipment, in particular to an automatic sample adding and mixing device for spectrophotometry pretreatment.
Background
The detection technology is one of the indispensable means of scientific experiments, the commonly used ultraviolet spectrophotometry is a qualitative, quantitative and structural analysis method established according to the absorption characteristics of substance molecules to electromagnetic waves with different wavelengths, and the spectrophotometer is used as optical detection equipment and is often used for detecting and analyzing the content of each element of a sample.
The spectrophotometry is divided into a pretreatment part and a detection part, wherein the workload of the pretreatment part accounts for about 80% of the whole process, however, in the spectrophotometry detection experiment, the manual operation is still needed to finish the working contents of the whole pretreatment such as sample weighing, sample adding liquid, uniform oscillation mixing, sample extracting, reagent adding and the like, and the manual operation is low in efficiency and inevitably causes operation errors due to more working links of the pretreatment part, so that the detection result of an upper machine is influenced. Therefore, an automatic sample adding and mixing device for pretreatment is needed, and the pretreatment work content in spectrophotometry is automatically completed, so that the purposes of saving trouble and labor and saving cost are achieved.
Disclosure of Invention
The invention provides an automatic sample adding and mixing device for spectrophotometry pretreatment, which aims to solve the technical problems that in spectrophotometry, the efficiency is low, the accuracy is poor, the detection result has errors, the labor cost is high and automatic detection cannot be realized.
The technical scheme provided by the invention is as follows:
The invention provides an automatic sample adding and mixing device for spectrophotometry pretreatment, which comprises a base, a power supply placed on the base, a transmission and mixing device for mixing samples, a sample adding and sampling device for adding buffering agents, a reagent adding device for adding experimental reagents, a weighing device for measuring the whole weight of a sample cup, a cleaning device for cleaning a sample adding and sampling needle and a reagent needle, a control terminal for collecting data and a control system arranged above the base, wherein the transmission and mixing device is fixedly arranged on the base through a supporting column, the sample adding and sampling device, the reagent adding device, the weighing device and the cleaning device are arranged around the transmission and mixing device, the control system is used for controlling the transmission and mixing device, the sample adding and sampling device, the reagent adding device, the weighing device and the cleaning device, and is also used for receiving instructions sent by the control terminal, and the control terminal is in the form of running control software in a network computer or a mobile phone.
The transmission mixing device comprises a first stepping motor, a first transmission belt, a rotating shaft, a tray and a tray support, wherein the tray support comprises a supporting column and a fixed platform, the supporting column is vertically arranged on a base, the fixed platform is horizontally and fixedly arranged at the top end of the supporting column, a shaft hole is formed in the center of the fixed platform, the rotating shaft penetrates through the shaft hole, a first bearing (not shown in the figure) is arranged in the shaft hole, the outer ring of the first bearing is embedded into the inner wall of the shaft hole, the inner ring of the first bearing is embedded into the surface of the rotating shaft, the tray is parallel to the fixed platform, the center of the tray is fixedly arranged at the top end of the rotating shaft, the first stepping motor is arranged below the fixed platform through an L-shaped fixing frame, and a driving shaft of the first stepping motor is vertically arranged and is in transmission connection with the bottom end of the rotating shaft through the first transmission belt.
The tray is preferably disc-shaped, a plurality of sample holes, a plurality of reagent holes and a plurality of cuvette holes are formed in the surface of the disc-shaped tray in a penetrating mode, the sample holes, the reagent holes and the cuvette holes are uniformly distributed around the axis of the center of the disc-shaped tray, the distance from the center of the cuvette holes to the axis is a, the distance from the center of the sample holes to the axis is b, the distance from the center of the reagent holes to the axis is c, a is less than or equal to b and less than or equal to c, a first connecting column is arranged on the lower bottom surface of the edge of the tray, the first connecting column is perpendicular to the tray, the bottom end of the first connecting column is connected with an arc-shaped supporting plate, the arc-shaped supporting plate is arranged between the tray and the fixing platform, the arc-shaped supporting plate is arranged under all the reagent holes, the bottom of the tray is also provided with a second connecting column, the bottom end of the second connecting column is connected with an annular shape, the annular shape is arranged between the tray and the fixing platform, and the annular supporting plate is arranged under all the color-comparison holes.
The heating plate is provided with a temperature sensor, a heating coil for heating is arranged in the heating plate, the heating plate is fixedly arranged at the center of the fixing platform, a through hole for the rotating shaft to pass through is formed in the center of the heating plate, the heating plate is provided with an annular groove for heating the cuvette, and the annular supporting plate is arranged in the annular groove.
The reagent adding device comprises a first mounting seat, a plunger pump, a second stepping motor, a first rotating rod, a first rotating arm and an L-shaped reagent needle, wherein the first mounting seat comprises a first lower plate surface, a first supporting plate and a first upper plate surface, the first lower plate surface is fixedly arranged on the base, the first upper plate surface is arranged right above the first lower plate surface, the first supporting plate is used for connecting the first upper plate surface and the first lower plate surface, the second stepping motor is fixedly arranged at the bottom of the first upper plate surface, a driving shaft of the second stepping motor penetrates through the first upper plate surface, the first rotating rod rotatably penetrates through the first upper plate surface, a driving shaft of the second stepping motor is in transmission connection with the first rotating rod through a second transmission belt, a third stepping motor is arranged at the bottom of the first lower plate surface, a driving shaft of the third stepping motor penetrates through the first supporting plate and is arranged at the tail end of the first driving wheel, a first driven wheel is arranged right above the first driving wheel, the first driven wheel and the first driving wheel is connected with the first driving belt, a driving shaft of the first stepping motor penetrates through the first driven wheel, a first rotating rod is fixedly arranged at the other end of the first rotating rod is connected with the L-shaped reagent needle, and the L-shaped reagent needle is fixedly arranged at one end of the first rotating rod through the first rotating rod, and the other end of the L-shaped reagent needle is fixedly penetrates through the first rotating rod, and the L-shaped needle.
Preferably, the sample adding and sampling device comprises a second mounting seat, a peristaltic pump, a fourth stepping motor, a second rotating rod, a second rotating arm and an L-shaped sample adding and sampling needle; the second mounting seat comprises a second lower plate surface, a second supporting plate and a second upper plate surface, the second lower plate surface is fixedly arranged on the base, the second upper plate surface is arranged right above the second lower plate surface, the second supporting plate is used for connecting the second upper plate surface and the second lower plate surface, the fourth stepping motor is fixedly arranged at the bottom of the second upper plate surface, a driving shaft of the fourth stepping motor penetrates through the second upper plate surface, the second rotating rod rotatably penetrates through the second upper plate surface, the driving shaft of the fourth stepping motor and the second rotating rod are in transmission connection through a fourth transmission belt, a fifth stepping motor is arranged at the bottom of the second lower plate surface, the driving shaft of the fifth stepping motor penetrates through the second supporting plate, a second driving wheel is arranged at the tail end of the second stepping motor, a second driven wheel is arranged right above the second driving wheel, the second driving wheel and the second driven wheel are in transmission connection through a fifth transmission belt, a third bearing is sleeved at the bottom end of the second stepping motor, a second transmission plate is fixedly arranged through the second bearing, the second transmission belt penetrates through the second rotating rod, the second peristaltic pump is fixedly arranged at the second rotating rod is connected with the other end of the second sample injection tube, and the second sample injection tube is fixedly connected with the other end of the sample injection tube.
The weighing device comprises a scale pan, a pressure sensor and a screw motor, wherein the screw of the screw motor is arranged in the vertical direction, a sliding block is slidably arranged on the screw of the screw motor, the upper end of the sliding block is connected with the pressure sensor, the upper end of the pressure sensor is connected with the scale pan, a sample groove is formed in the fixed platform penetrating through the surface of the fixed platform, the scale pan penetrates through the sample groove, and the scale pan is arranged right below the sample hole.
Preferably, the cleaning device comprises a cleaning tank arranged on the fixed platform, a first diaphragm pump and a second diaphragm pump arranged on the base, wherein the first diaphragm pump is provided with a waste liquid pipe and a liquid discharge pipe;
The second diaphragm pump is provided with a water inlet pipe and a water outlet pipe, one end of the water inlet pipe is connected with a clean water tank, the other end of the water inlet pipe is connected with the second diaphragm pump, the water outlet pipe is connected with the second diaphragm pump and the cleaning tank, a branch pipe is connected to the water outlet pipe, an electromagnetic valve is arranged on the branch pipe, and the tail end of the branch pipe is connected to the plunger pump.
Preferably, the control system comprises a control main board and a data exchanger connected with the control main board, wherein the connection mode of the control terminal and the control main board is wireless connection or electric connection, the data exchanger comprises a temperature control module and a driving module, the temperature control module is connected with the temperature sensor and used for controlling the temperature of the heating disc, and the first driving module is used for controlling the operation of the first stepping motor, the second stepping motor, the third stepping motor, the fourth stepping motor, the fifth stepping motor, the plunger pump, the peristaltic pump, the diaphragm pump and the screw motor.
The invention has the beneficial effects that:
The automatic sample adding and mixing device for spectrophotometry pretreatment provided by the invention has the advantages that the automatic sample adding and mixing device for spectrophotometry pretreatment, the sample adding and sampling device, the transmission and mixing device, the weighing device, the reagent adding device, the cleaning device, the control system and the control terminal are matched for use, so that the automatic addition of samples and reagents of a pretreatment part cuvette in spectrophotometry is realized, the efficiency and the accuracy are high, the error is small, the cost of manual sample adding of professional experimenters is reduced, and the cost is saved.
Drawings
The invention and its features, aspects and advantages will become more apparent from the detailed description of non-limiting embodiments with reference to the following drawings. Like numbers refer to like parts throughout. The drawings are not intended to be drawn to scale, emphasis instead being placed upon illustrating the principles of the invention.
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a perspective view of another angle of the present invention;
FIG. 3 is a front view of the present invention;
FIG. 4 is a top view of the present invention with respect to a stationary platform;
FIG. 5 is a perspective view of a weighing apparatus according to the present invention;
FIG. 6 is a perspective view of the present invention with respect to a transmission blending device;
FIG. 7 is a perspective view showing a reagent adding apparatus according to the present invention;
Detailed Description
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application.
It will be understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, steps, operations, devices, components, and/or groups thereof.
The terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship based on that shown in the drawings, merely for convenience of description and to simplify the description, and do not denote or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the invention.
The terms "first," "second," "third," and the like, as used herein, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Unless specifically stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected, mechanically connected, electrically connected, directly connected, or indirectly connected via an intervening medium, or may be in communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
The following description of the technical solutions according to the embodiments of the present invention refers to the accompanying drawings, which are included to illustrate only some embodiments of the invention, and not all embodiments. Accordingly, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by a person skilled in the art without making any inventive effort, are intended to fall within the scope of the present invention.
Example 1
In order to solve the technical problems that in a spectrophotometry method, the efficiency is low, the accuracy is poor, the detection result has errors, the labor cost is high, and the automatic detection cannot be realized.
The embodiment of the invention provides an automatic sample adding and mixing device for spectrophotometry pretreatment, which comprises a base 1, a power supply 2 (power supply for the whole device) arranged on the base 1, a transmission and mixing device 3 for mixing samples, a sample adding and sampling device 4 for adding buffering agents, a reagent adding device 5 for adding experimental reagents, a weighing device 6 for measuring the whole weight of a sample cup, a cleaning device 7 for cleaning a sample adding and sampling needle 406 and a reagent needle 506, a control terminal 8 for collecting data and a control system 9 arranged above the base 1, wherein the transmission and mixing device 3 is fixedly arranged on the base 1 through a support column 10, the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6 and the cleaning device 7 are arranged around the transmission and mixing device 3, the control system 9 is used for controlling the transmission and mixing device 3, the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6 and the cleaning device 7, the control system 9 is also used for receiving instructions sent by the control terminal 8, and the control terminal 8 is in the form of running control software in a network computer or a mobile phone.
In this embodiment, the transmission blending device 3 preferably comprises a first stepping motor 301, a first transmission belt 302, a rotating shaft 303, a tray 304 and a tray support 305, wherein the tray support 305 comprises a support column 10 and a fixed platform 11, the support column 10 is vertically installed below the fixed platform 11, the fixed platform 11 is horizontally fixedly arranged at the top end of the support column 10, a shaft hole is formed in the center of the fixed platform 11, the rotating shaft 303 penetrates through the shaft hole, a first bearing is arranged in the shaft hole, an outer ring of the first bearing is embedded in the inner wall of the shaft hole, an inner ring of the first bearing is embedded in the surface of the rotating shaft 303, so that the tray 304 can rotate under the action of the first stepping motor 301, the fixed platform 11 cannot be arranged, the tray 304 is arranged parallel to the fixed platform 11, the center of the tray 304 is fixedly arranged at the top end of the rotating shaft 303, the first stepping motor 301 is in transmission connection with the bottom end of the rotating shaft 303 through an L-shaped fixing frame 12, the first stepping motor 301 receives instructions from a driving module 33, and is driven by the first transmission belt 302, the first bearing is embedded in the inner ring of the rotating shaft 303, the inner ring of the first bearing is embedded in the surface of the rotating shaft 303, the tray is arranged on the rotating shaft 303, and the driving shaft can synchronously move with the driving shaft and the driving shaft can synchronously move, and the driving shaft can be added on the bottom of the driving shaft and the driving shaft can realize the driving and the driving movement can synchronously.
In this embodiment, the tray 304 is preferably disk-shaped; the surface of the disk-shaped tray 304 is provided with a plurality of sample holes 13, a plurality of reagent holes 14 and a plurality of cuvette holes 15 in a penetrating manner, sample cups, reagent bottles and cuvettes are respectively placed in the sample holes 13, the reagent holes 14 and the cuvette holes 15, the sample holes 13, the reagent holes 14 and the cuvette holes 15 are uniformly distributed around the axis of the center of the disk-shaped tray 304, the distance from the center of the cuvette holes 15 to the axis is a, the distance from the center of the sample holes 13 to the axis is b, the distance from the center of the reagent holes 14 to the axis is c, a is equal to or less than b, in spectrophotometry experiments, the diameters of the sample cups are generally larger than the diameter of the cuvette holes, the number of the cuvette cups is required to be consistent, the sample holes 13 for placing the sample cups are required to be arranged on the outer ring-shaped tray 304, the reagent holes 14 are arranged on the outermost ring-shaped tray 304 for being matched with the use of reagent needles 506, the first connecting column 16 is arranged on the bottom surface of the disk-shaped tray 304, the bottom of the first connecting column 16 is perpendicular to the tray 304, the bottom end of the first connecting column 16 is connected with the arc 17, the first connecting column 16 is arranged between the center of the sample holes 13 and the axis, the axis is c, the distance from the center of the reagent holes 14 is equal to the axis is equal to c, the diameter of the sample cups is equal to or less than c, in the diameter of the cuvette holes are generally equal to the cuvette holes, the cuvette holes are generally larger than the diameter of the cuvette holes are larger than the cuvette holes, the cuvette holes are required to be placed on the outer ring-shaped tray 304, the cuvette holes 13, and the cuvette holes are respectively, and are arranged on the outer ring-shaped and the cuvette holes are respectively.
In this embodiment, it is preferable that the fixed platform 11 is provided with a heating plate 20, a temperature sensor is provided on the heating plate 20, a heating coil (not shown in the figure) for heating is provided in the heating plate 20, the heating plate 20 is fixedly provided at the center of the fixed platform 11, the environmental temperature in winter is low, the activity of various reagents can be affected, in order to avoid inconvenience caused by the environmental temperature to spectrophotometry experiments, a heating plate cuvette is used for heating, a through hole for passing through a rotating shaft 303 is provided at the center of the heating plate 20, an annular groove 21 for heating the cuvette is provided in the heating plate 20, an annular supporting plate 19 is provided in the annular groove 21, and each cuvette is heated and kept in the groove of the annular groove 21.
Preferably in this embodiment, the reagent adding apparatus 5 comprises a first mount 501, a plunger pump 502, a second stepper motor 503, a first rotary lever 504, a first rotary arm 505, and an "L-shaped" reagent needle 506; the first mounting seat 501 comprises a first lower plate surface, a first supporting plate and a first upper plate surface, wherein the first lower plate surface is fixedly arranged on the base 1, the first upper plate surface is arranged right above the first lower plate surface, the first supporting plate is used for connecting the first upper plate surface and the first lower plate surface, the second stepping motor 503 is fixedly arranged at the bottom of the first upper plate surface, a driving shaft of the second stepping motor 503 penetrates through the first upper plate surface, a first rotating rod 504 is rotatably arranged through the first upper plate surface, the driving shaft of the second stepping motor 503 and the first rotating rod 504 are in transmission connection through a second transmission belt 22, a third stepping motor 23 is arranged at the bottom of the first lower plate surface, a driving shaft of the third stepping motor 23 penetrates through the first supporting plate, the end of the first driving wheel 24 is arranged, a first driven wheel 25 is arranged right above the first driving wheel 24, the first driving wheel 24 and the first driven wheel 25 are in transmission connection through a third transmission belt 26, a second bearing is sleeved at the bottom of the first rotating rod 504, a first transmission plate 28 is fixedly arranged on the outer ring of the second bearing, the first transmission plate 28 is fixedly connected with one side of the third transmission belt 26, one end of the first rotating rod 505 is fixedly arranged at the top end of the first rotating rod 504, a needle-shaped rotating rod 506 penetrates through the first L-shaped rotating rod 506, a reagent injection tube 506 penetrates through the other end of the first rotating rod 506, and a reagent injection tube 27 is arranged at the other end of the first rotating rod segment penetrating through the needle segment of the first rotating rod 506. The second stepping motor 503 and the third stepping motor 23 receive instructions from the driving module 33, the second stepping motor 503 realizes synchronous movement of the first rotating rod 504 and the driving shaft through the second transmission belt 22, lifting of the first rotating rod 504 is realized through the third stepping motor 23, the relative positions of the first driving wheel 24 and the first driven wheel 25 on the driving shaft of the third stepping motor 23 are unchanged, the third transmission belt 26 drives the first transmission plate 28 arranged on the third stepping motor to move up and down under the action of friction force, so that the second bearing and the first rotating rod 504 arranged on the second bearing are driven to lift, the reagent needle 506 is matched with the reagent tube 17 to use, and the plunger pump 502 sucks reagent in the reagent bottle and keeps the reagent at the front end of the reagent tube 17, so that the reagent can be conveniently injected into each cuvette subsequently.
In this embodiment, the sample application and sampling device 4 preferably includes a second mount 401, a peristaltic pump 402, a fourth stepper motor 403, a second rotary rod 404, a second rotary arm 405, and an "L-shaped" sample application and sampling needle 406; the second mounting seat 401 comprises a second lower plate surface, a second supporting plate and a second upper plate surface, wherein the second lower plate surface is fixedly arranged on the base 1, the second upper plate surface is arranged right above the second lower plate surface, the second supporting plate is used for connecting the second upper plate surface and the second lower plate surface, a fourth stepping motor 403 is fixedly arranged at the bottom of the second upper plate surface, a driving shaft of the second stepping motor 403 penetrates through the second upper plate surface, a second rotating rod 404 is rotatably arranged through the second upper plate surface, a driving shaft of the fourth stepping motor 403 is in transmission connection with the second rotating rod 404 through a fourth transmission belt, a fifth stepping motor is arranged at the bottom of the second lower plate surface, a driving shaft of the fifth stepping motor penetrates through the second supporting plate, a second driving wheel is arranged at the tail end of the fifth stepping motor, a second driven wheel is arranged right above the second driving wheel, the second driving wheel is in transmission connection with the second driven wheel through a fifth transmission belt, a third bearing is sleeved at the bottom end of the second rotating rod 404, a second transmission plate is fixedly arranged at the outer ring of the third bearing, the second transmission plate is fixedly connected with one side of the fifth transmission belt, one end of the second rotating rod 405 is fixedly arranged at the top end of the second rotating rod 404, an L-shaped sample injection needle 406 penetrates through the second sample injection needle 29, and the other end of the L-shaped sample injection needle 404 is connected with the other end of the second sample injection needle 404. The fourth stepping motor and the fifth stepping motor receive instructions from the driving module 33, the fourth stepping motor realizes synchronous movement of the driving shaft and the first rotating rod 404 through a fourth transmission belt, lifting of the first rotating rod 404 is realized through the fifth stepping motor, the relative positions of a second driving wheel and a second driven wheel on the driving shaft of the fifth stepping motor are unchanged, the fifth transmission belt drives a second transmission plate arranged on the fifth driving wheel to move up and down under the action of friction force, so that a third bearing and the first rotating rod 404 arranged on the third bearing are driven to lift, a sample adding and sampling needle 406 is matched with a sample liquid pipe 29 to use, a peristaltic pump 402 absorbs sample liquid in a sample cup, and the sample liquid is stored at the front end of the sample liquid pipe 29, so that the sample liquid can be conveniently injected into a corresponding cuvette in a subsequent mode.
In this embodiment, preferably, the weighing device 6 includes a scale 601, a pressure sensor 602 and a screw motor 603, wherein the screw of the screw motor 603 is arranged along the vertical direction, a sliding block 30 is slidably arranged on the screw of the screw motor 603, the upper end of the sliding block 30 is connected with the pressure sensor 602, the upper end of the pressure sensor 602 is connected with the scale 601, a sample groove 31 is formed in the fixed platform 11 penetrating through the surface of the fixed platform, the scale 601 penetrates through the sample groove 31, and the scale 601 is arranged right below the sample hole 13. In the spectrophotometry experiment process, the first sample cup with the sample is weighed, the weight of the sample cup accords with a set range, the tray 304 rotates, and the next sample cup with the sample is weighed.
In the embodiment, the cleaning device 7 comprises a cleaning tank 701 arranged on a fixed platform, a first diaphragm pump 702 and a second diaphragm pump 703 arranged on a base, wherein the first diaphragm pump 702 is provided with a waste liquid pipe 34 and a liquid discharge pipe 36, the waste liquid pipe 34 is connected with the cleaning tank 701 and the first diaphragm pump 702, one end of the liquid discharge pipe 36 is connected with the first diaphragm pump 702, and the other end is connected with the waste liquid tank 40;
the second diaphragm pump 703 is provided with a water inlet pipe 38 and a water outlet pipe 39, one end of the water inlet pipe 38 is connected with the clean water tank 41, the other end of the water inlet pipe 38 is connected with the second diaphragm pump 703, the water outlet pipe 39 is connected with the second diaphragm pump 703 and the cleaning tank 701, the water outlet pipe 39 is connected with a branch pipe 37, the branch pipe 37 is provided with an electromagnetic valve 35, and the tail end of the branch pipe 37 is connected with the plunger pump 502.
The second diaphragm pump 703 sucks clean water into the clean water tank 701 from the clean water tank 41, the first diaphragm pump 702 pumps the polluted clean water into the waste liquid tank 40, the clean water in the clean water tank 701 is replaced immediately, the pollution degree of the clean water can be reduced to the minimum, the first diaphragm pump 702 and the second diaphragm pump 703 are started simultaneously, the total operation time length of the first diaphragm pump 702 is equal to or longer than the total operation time length of the second diaphragm pump 703, the clean water in the clean water tank 701 is prevented from overflowing, the outer wall of the reagent needle 506 only needs to extend into the clean water tank 701 for cleaning, the cleaning mode of the inner wall of the reagent needle 506 is as follows, the electromagnetic valve 37 is opened, the clean water is led into the branch pipe 37, and the plunger pump 502 pumps the clean water in the branch pipe 37 into the reagent needle 506 and discharges the clean water into the clean water tank 701, so as to clean the inner wall of the reagent needle 506.
In this embodiment, the control system 9 preferably includes a control main board 901 and a data exchanger 902 connected to the control main board 901, where the connection mode between the control terminal 8 and the control main board 901 is wireless connection or electrical connection, the data exchanger 902 includes a temperature control module 32 and a driving module 33, the temperature control module 32 is connected to a temperature sensor and is used for controlling the temperature of the heating disc 20, and the first driving module 33 is used for controlling the operation of the first stepping motor 301, the second stepping motor 503, the third stepping motor 23, the fourth stepping motor 403, the fifth stepping motor, the plunger pump 502, the peristaltic pump 402, the diaphragm pump 702 and the lead screw motor 603, so as to control the use of the transmission mixing device 3, the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6 and the cleaning device 7.
The working steps of the automatic sample adding and mixing device for spectrophotometry pretreatment related to the embodiment are as follows:
1. The scale pan is lifted to the highest point, a sample is placed in a sample cup, then the sample cup is placed in a sample hole 13 marked with a number one position above the scale pan 601, the sample cup falls on the scale pan 601, the sample cup in the number one sample hole 13 is marked as a number one sample cup, the number one sample cup with the sample is weighed, the weight of the sample cup accords with a set range, a tray 304 rotates, the next sample cup with the sample is weighed, the weight of the sample cup is not in the set range, the tray 304 stops rotating;
2. The second rotating rod rotates to drive the sample adding and sampling needle 406 to move to the position right above the sample cups, buffer solution is added to all sample cups in sequence, the buffer solution is added according to the actual weight of samples in each sample cup, namely, the buffer solution is added proportionally, all the sample adding and sampling needle 406 is ready above the tray 304, the tray 304 rotates clockwise alternately to fully mix the samples in the sample cups with the buffer solution, the sample adding and sampling needle 406 moves to the position above the sample cup with a certain height, the sample liquid in the sample cup with a certain height is sucked, then the sample adding and sampling needle 406 rises and rotates to the position above the cuvette with a certain height, the sucked sample liquid is injected into the cuvette with a certain size, the sample adding and sampling needle 406 moves to the cleaning pond 701 to be cleaned, the sample liquid in each sample cup is respectively injected into each cuvette until the sample liquid in all the sample cups is extracted, the tray 304 is reset to the initial position, the sample adding and sampling needle 406 is washed in the cleaning pond after the sample adding and sampling needle 406 is ready above the cleaning pond 701;
3. The reagent needle 506 is cleaned, the reagent needle 506 stands by above the cleaning pool 701 after the cleaning is finished, reagent bottles filled with different reagents are respectively placed in the reagent holes 14, a first rotary rod drives the reagent needle 506 to move above one of the reagent bottles, a certain amount of reagents in the reagent bottles are sucked, the reagents required at the time are sucked into the reagent tube 27 all at one time, the reagent needle 506 moves above a cuvette, the reagents are injected into the cuvette of the first size, the positions of the reagent needle 506 are kept still, the tray 304 is rotated, the reagents are sequentially injected into the cuvettes in a one-suction-multiple-beating mode, after all the adding is finished, the reagent needle 506 is rotated to the cleaning pool 701 for cleaning, after the cleaning is finished, the first rotary rod drives the reagent needle 506 to move, the second reagent is sucked into the cuvette one by one, after all the adding is finished, the reagent needle 506 is rotated to the cleaning pool 701 for cleaning, the reagents in the rest reagent bottles are injected into the cuvettes one by one, the tray 304 can be rotated clockwise alternately according to whether the experiment requirement is required or not after the tray 304 is completely added with one reagent.
The above-described embodiments are merely illustrative of the principles of the present application and their effectiveness, and are not intended to limit the present application. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present application. Accordingly, it is intended that all equivalent modifications and variations which may be accomplished by those skilled in the art without departing from the spirit and technical spirit of the present invention shall be covered by the claims of this patent application.
Claims (9)
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| CN208568811U (en) * | 2018-05-07 | 2019-03-01 | 珠海市丽拓生物科技有限公司 | High-precision full-automatic novel urine iodine detector |
| CN111044743A (en) * | 2020-01-06 | 2020-04-21 | 无锡集佳智能科技有限公司 | Full-automatic sample pretreatment device |
| CN211955516U (en) * | 2020-03-18 | 2020-11-17 | 贵州传向医疗器械有限公司 | Integrated detection device for medical biochemical detection |
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| JP2775082B2 (en) * | 1992-04-14 | 1998-07-09 | 花王株式会社 | Sample weighing method using automatic analyzer |
| CN105929185B (en) * | 2016-06-21 | 2018-03-06 | 鹿钦宇 | Full automatic biochemical apparatus and its reagent adding method |
| CN108152285A (en) * | 2018-02-10 | 2018-06-12 | 王鲁生 | A kind of fully-automatic intelligent urine iodine analyzer and its method of work |
| CN109142766B (en) * | 2018-09-28 | 2024-11-08 | 基蛋生物科技股份有限公司 | Chemiluminescence detector |
| CN111044742B (en) * | 2020-01-06 | 2025-11-21 | 无锡集佳智能科技有限公司 | Intelligent sample processing and detecting device |
| CN212275566U (en) * | 2020-04-24 | 2021-01-01 | 江苏中朗宏泰科学仪器有限公司 | Reagent adds mechanism and has pesticide residue detector of this mechanism |
| CN111856056B (en) * | 2020-08-05 | 2024-12-20 | 丹东百特仪器有限公司 | A fully automatic sample addition system for high-throughput laser particle size analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN208568811U (en) * | 2018-05-07 | 2019-03-01 | 珠海市丽拓生物科技有限公司 | High-precision full-automatic novel urine iodine detector |
| CN111044743A (en) * | 2020-01-06 | 2020-04-21 | 无锡集佳智能科技有限公司 | Full-automatic sample pretreatment device |
| CN211955516U (en) * | 2020-03-18 | 2020-11-17 | 贵州传向医疗器械有限公司 | Integrated detection device for medical biochemical detection |
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