CN113796218A - Rapid callus rooting system and rapid callus rooting method for cuttings - Google Patents
Rapid callus rooting system and rapid callus rooting method for cuttings Download PDFInfo
- Publication number
- CN113796218A CN113796218A CN202111243448.2A CN202111243448A CN113796218A CN 113796218 A CN113796218 A CN 113796218A CN 202111243448 A CN202111243448 A CN 202111243448A CN 113796218 A CN113796218 A CN 113796218A
- Authority
- CN
- China
- Prior art keywords
- callus
- liquid
- rooting
- water tank
- rapid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
- A01G9/16—Dismountable or portable greenhouses ; Greenhouses with sliding roofs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
- A01G9/246—Air-conditioning systems
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
- A01G9/247—Watering arrangements
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Hydroponics (AREA)
Abstract
本发明公开了一种扦插枝条快速愈伤生根系统及快速愈伤生根方法，涉及农业生产技术领域，包括培养室，培养室内设有空气温度自动调节系统和诱导愈伤装置，诱导愈伤装置包括依次连接的水箱、雾化器和定植管，空气温度自动调节系统用于控制培养室的温度，定植管用于固定扦插枝条的下部，水箱内设置有自动加热装置，水箱内用于盛放促进形成愈伤组织的液体或促进生根的液体，自动加热装置用于加热水箱内盛放的液体，雾化器用于将水箱内盛放的液体喷入定植管中，雾化器通过时控开关控制开启和关闭。本发明使扦插枝条的下部处于雾化环境中，氧气充足；扦插枝条的下部的温度为最适宜形成愈伤组织的温度，枝条的上部温度相对较低，利于提高枝条扦插成活率。
The invention discloses a rapid callus rooting system and a rapid callus rooting method for cuttings, and relates to the technical field of agricultural production. The water tank, atomizer and planting tube are connected in sequence. The air temperature automatic adjustment system is used to control the temperature of the culture room. The planting tube is used to fix the lower part of the cuttings. The water tank is provided with an automatic heating device. The liquid of callus or the liquid that promotes rooting, the automatic heating device is used to heat the liquid contained in the water tank, the atomizer is used to spray the liquid contained in the water tank into the planting tube, and the atomizer is controlled by the time control switch to open and off. In the present invention, the lower part of the cutting branch is placed in an atomized environment, and the oxygen is sufficient; the temperature of the lower part of the cutting branch is the temperature most suitable for forming callus, and the temperature of the upper part of the branch is relatively low, which is beneficial to improve the survival rate of the cutting branch.
Description
Technical Field
The invention relates to the technical field of agricultural production, in particular to a rapid callus rooting system and a rapid callus rooting method for cuttage branches.
Background
The traditional fruit tree branch cuttage has the following problems:
1. the base of the branch is planted in soil or substrate which keeps a certain temperature and humidity, but the problem is that the temperature of the air at the upper part of the planted branch is high and the temperature of the soil or substrate at the lower part of the planted branch is low under the common conditions. Leading the upper lateral buds of the cuttage branches to sprout in advance, consuming nutrition and moisture of the branches, leading the lower temperature of the cuttage branches to be lower, leading callus not to be easy to generate and causing cuttage failure;
2. the branches are easy to rot and necrose due to low temperature and high humidity in soil or matrix for cuttage at the bases of the branches;
3. the branches are cut in soil or a substrate, and because the humidity is too high, the branches cannot be sterilized thoroughly, and the soil contains a large number of insect pests, so that underground pests such as fly maggots, root-knot nematodes and the like are easy to occur, and the branches are easy to necrotize.
4. The branches are planted in soil or matrix in a cuttage mode, the air permeability of roots is poor, callus is not generated easily, and the efficiency and the quality of cuttage seedling raising of woody plants such as fruit trees and the like are seriously influenced.
Disclosure of Invention
The invention aims to provide a rapid callus and rooting system and a rapid callus and rooting method for cutting branches, which are used for solving the problems in the prior art, so that the lower parts of the cutting branches are positioned in an atomized environment of nutrient solution and rooting agent, and oxygen is sufficient; the temperature of the lower part of the cuttage branch is the most suitable temperature for forming callus, the temperature of the upper part of the branch is relatively low, the upper axillary buds of the cuttage branch are prevented from germinating prematurely, and the branch cuttage survival rate is favorably improved.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a rapid callus and rooting system for cutting branches, which comprises a culture chamber, wherein an automatic air temperature regulating system, an induced callus device and a controller are arranged in the culture chamber, the induced callus device comprises a water tank, an atomizer and a field planting pipe which are sequentially connected, the automatic air temperature regulating system is used for controlling the temperature of the culture chamber, the field planting pipe is used for fixing the lower part of the cutting branches, an automatic heating device is arranged in the water tank, the water tank is used for containing liquid for promoting the formation of callus or liquid for promoting rooting, the automatic heating device is used for heating the liquid contained in the water tank, the atomizer is used for spraying the liquid contained in the water tank into the field planting pipe, the atomizer is controlled to be opened and closed by a time control switch, and the controller, the automatic air temperature regulating system, the controller and the field planting pipe are controlled to be opened and closed by the time control switch, The automatic heating device is electrically connected with the time control switch.
Preferably, a liquid discharge pipe is connected to the planting pipe and used for discharging liquid in the planting pipe.
Preferably, the atomizer with the field planting pipe passes through the spray pipe intercommunication, be provided with a plurality of shower nozzles on the spray pipe, each shower nozzle all is located in the field planting pipe.
Preferably, the lower part of each cutting branch is provided with a sponge block, and each sponge block is positioned in the planting pipe.
Preferably, the field planting pipe is horizontally arranged, a plurality of field planting holes are uniformly formed in the field planting pipe, and the field planting holes are used for the lower parts of the cuttage branches to penetrate through.
Preferably, the culture chamber comprises a frame body, and a black and white film is arranged on the outer side of the frame body.
The invention also provides a rapid callus rooting method adopting the rapid callus rooting system for the cutting branches, which comprises the following steps:
step one, opening an automatic air temperature regulating system to control the temperature in a culture chamber to be 15-18 ℃, opening an automatic heating device, and heating liquid which promotes callus formation in a water tank to 35-40 ℃;
spraying atomized liquid for promoting the lower part of the cuttage branch to form callus into the planting pipe, and ensuring the upper part of the cuttage branch to be under the condition of weak scattered light illumination at the temperature of 18-20 ℃; spraying for 2 minutes every 1h to enable the lower part of the cuttage branch to be in a constant-temperature high-humidity state with the temperature of 25-32 ℃ and the humidity of more than or equal to 90%, and further inducing the lower part of the cuttage branch to generate callus;
step three, after the lower part of the cuttage branch forms callus, replacing the liquid in the water tank with the liquid for promoting rooting, opening the automatic heating device, and heating the liquid in the water tank to 35-40 ℃;
spraying vaporous liquid for promoting rooting into the planting tube, ensuring the illumination condition until the lower part of the cuttage branch takes root, and spraying for 2min every 1h all day to ensure that the lower part of the cuttage branch is in a constant temperature and high humidity state, so as to promote the induction of callus to generate adventitious roots.
Preferably, in the first step, the liquid for promoting callus formation comprises modified hopcalite nutrient solution and naphthylacetic acid, wherein the modified hopcalite nutrient solution comprises 472.5mg/L calcium nitrate tetrahydrate, 253mg/L potassium nitrate, 40mg/L ammonium nitrate, 68mg/L monopotassium phosphate, 246.5mg/L magnesium sulfate, 1.39mg/L ferrous sulfate heptahydrate, 1.865mg/L disodium edetate, 3.1mg/L boric acid, 11.15mg/L manganese sulfate, 4.3mg/L zinc sulfate, 0.415mg/L potassium iodide, 0.125mg/L sodium molybdate, 0.013mg/L copper sulfate and 0.013mg/L cobalt chloride, and the naphthylacetic acid is 100PPM calculated according to 0.5 times of standard nutrient solution concentration.
Preferably, the liquid for promoting the formation of the callus is also added with 10000 times of 90% carbendazim water dispersible granules and/or 3000 times of 1.8% abamectin water emulsion, so that the occurrence of germ infection and insect pest can be effectively prevented and treated.
Preferably, the liquid for promoting rooting comprises the modified Hoagland nutrient solution and indole butyric acid, and the indole butyric acid is 100 PPM.
Compared with the prior art, the invention has the following technical effects:
the invention is applied to the rapid propagation of fruit tree branch cuttage, and comprises tree species which are easy to root by cuttage, such as fig, grape, pomegranate, medlar, and south Xinjiang special fruit trees which are difficult to root by cuttage, such as jujube, apricot, peach, apple, bergamot pear, and the like.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram of a rapid callus rooting system for cutting shoots according to the present invention;
FIG. 2 is a schematic view of an atomizer according to the present invention;
wherein: 100-a rapid callus and rooting system for cutting branches, 1-a culture room, 2-an automatic air temperature adjusting system, 3-an induced callus device, 4-a water tank, 5-an atomizer, 6-a planting tube, 7-an automatic heating device, 8-a liquid discharge tube, 9-a spraying pipeline, 10-a spray head, 11-a controller and 12-a time control switch.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
The invention aims to provide a rapid callus and rooting system and a rapid callus and rooting method for cutting branches, which are used for solving the problems in the prior art, so that the lower parts of the cutting branches are positioned in an atomized environment of nutrient solution and rooting agent, and oxygen is sufficient; the temperature of the lower part of the cuttage branch is the most suitable temperature for forming callus, the temperature of the upper part of the branch is relatively low, the upper axillary buds of the cuttage branch are prevented from germinating prematurely, and the branch cuttage survival rate is favorably improved.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example one
As shown in fig. 1-2: the embodiment provides a rapid cuttage branch callus and rooting system 100, which comprises a culture chamber 1, wherein an automatic air temperature adjusting system 2, an induction callus device 3 and a controller 11 are arranged in the culture chamber 1, the induction callus device 3 comprises a water tank 4, an atomizer 5 and a planting tube 6 which are sequentially connected, the automatic air temperature adjusting system 2 is used for controlling the temperature of the culture chamber 1, the automatic air temperature adjusting system 2 adopts four vertical air conditioners, the planting tube 6 is used for fixing the lower part of a cuttage branch, an automatic heating device 7 is arranged in the water tank 4, the water tank 4 is used for containing liquid for promoting callus formation or liquid for promoting rooting, the automatic heating device 7 is used for heating liquid contained in the water tank 4, the automatic heating device 7 adopts a heating rod with the power of 20KW for field planting and is provided with a temperature control device, the atomizer 5 is used for spraying the liquid contained in the water tank 4 into the tube 6, the atomizer 5 is controlled to be turned on and off by a time control switch 12, and a controller 11 is electrically connected with the automatic air temperature regulating system 2, the automatic heating device 7 and the time control switch 12.
The controller 11 controls the air temperature automatic regulating system 2, the automatic heating device 7 and the time control switch 12 to work, and the control process of the controller 11 is the prior art.
The temperature of the liquid in the water tank 4 is adjusted by the automatic heating device 7, so that the temperature of the generated atomized water vapor reaches 25-28 ℃; the automatic air temperature regulating system 2 regulates the indoor air temperature of the culture room 1 to be 15-18 ℃, so that the upper part of the branch is in a low temperature state, and the lower part of the cutting branch is in a high temperature state.
In this embodiment, atomizer 5 and field planting pipe 6 pass through spray pipe 9 intercommunication, are provided with a plurality of shower nozzles 10 on spray pipe 9, and each shower nozzle 10 all is located field planting pipe 6. The atomizer 5 adopts an ultrasonic atomizer, and the output force of the spray head 10 is 24 kg/h.
The water tank 4, the atomizer 5 and the planting pipe 6 are connected through pipelines to form a closed system, and the liquid for promoting the formation of callus or the liquid for promoting rooting is mutually converted in two forms of water and steam fog. Spraying every 1h for 2min throughout the day.
In this embodiment, the planting pipe 6 is connected with a liquid discharge pipe 8, and the liquid discharge pipe 8 is used for discharging the liquid in the planting pipe 6. The liquid is atomized by the atomizer 5, is transmitted to the lower part of the cuttage branch through the atomizing pipeline 9, and flows out of the liquid discharge pipe 8 at the far end of the planting pipe 6, and is not recycled, so that the secondary pollution of the liquid is prevented.
In the embodiment, the lower part of each cutting branch is provided with a sponge block 5-10cm, each sponge block is positioned in the field planting pipe 6, and the lower part of each cutting branch is fixed in the field planting pipe 6 through the sponge block.
In this embodiment, the field planting pipe 6 is horizontally arranged, and a plurality of field planting holes are evenly formed in the field planting pipe 6 and used for penetrating through the lower portion of the cuttage branch. The field planting pipe 6 is a white PVC pipe with the diameter of 110mm, the length of the field planting pipe 6 is 4m, the diameter of the field planting holes is 2.5cm, and the distance between the field planting holes is 10 cm.
In this embodiment, the culture chamber 1 includes a frame body, the frame body is built by stainless steel frames, the size of the frame body is 12m long by 4m wide by 3m high, a black and white film is arranged on the outer side of the frame body, and a passage door is arranged on one side of the culture chamber 1. The cultivation room 1 is internally provided with a passage with the length of 8m, four layers of three rows of planting pipes 6 are vertically arranged on two sides of the passage, and each layer of planting pipes has 3840 planting holes, so that the space is effectively utilized.
In this embodiment, the water tank 4 is located below ground level and has a capacity of 2 tons.
In the embodiment, the liquid in the water tank 4 is atomized by the atomizer 5 and then is delivered to the lower part of the cuttage branch through the spray pipeline 9; the lower part of the cutting branch is in an atomization environment with high temperature (25-28 ℃), high humidity (more than or equal to 90%), sterility, sufficient nutrition, sufficient oxygen and special hormone level, so that the environmental condition which is most favorable for the lower part of the cutting branch to quickly generate callus is created; the hormone for promoting rooting is replaced after the callus is generated, so that the rapid rooting is facilitated; the air temperature of the upper part of the branch is lower (15-18 ℃), the germination of the upper part of the branch is inhibited, and the death of the branch caused by the pumping due to the excessive nutrient and moisture consumption of the branch is prevented.
The cuttage branch rapid callus rooting system 100 is installed in a sunlight greenhouse of a gardening test station of Tarim university in Alarler city, Xinjiang, the time for cuttage of figs and the success rate of generation of callus through branch cuttage within 5-7 days are shortened by half compared with the traditional method, and the success rate is improved from 50-70% to more than 95%; the survival rate of the jujube branches which are difficult to root after cuttage is improved to more than 80 percent from 0 to 20 percent.
Example two
The embodiment provides a rapid callus rooting method adopting the rapid callus rooting system 100 for cutting branches of the first embodiment, which comprises the following steps:
step one, turning on an automatic air temperature regulating system 2 to control the temperature in a culture chamber 1 to be 15-18 ℃, turning on an automatic heating device 7 to heat the liquid promoting callus formation in a water tank 4 to 35-40 ℃;
spraying atomized liquid for promoting the lower part of the cuttage branch to form callus into the planting pipe 6, and ensuring the upper part of the cuttage branch to be under the condition of weak scattered light illumination at the temperature of 18-20 ℃; spraying for 2 minutes every 1h to enable the lower part of the cuttage branch to be in a constant-temperature high-humidity state with the temperature of 25-32 ℃ and the humidity of more than or equal to 90%, and further inducing the lower part of the cuttage branch to generate callus;
step three, after the lower part of the cuttage branch forms callus, replacing the liquid in the water tank 4 with the liquid for promoting rooting, opening the automatic heating device 7, and heating the liquid in the water tank 4 to 35-40 ℃;
spraying atomized liquid for promoting rooting into the planting pipe 6, ensuring the illumination condition until the lower part of the cuttage branch takes root, and spraying for 2min every 1h all day to ensure that the lower part of the cuttage branch is in a constant temperature and high humidity state, so as to promote the callus to generate adventitious roots.
The callus rooting time required among varieties is different, such as fig and grape which are easy to root form callus for one week, and the callus rooting starts for two weeks; the time of the jujubes and apples which are not easy to root is prolonged by about one time. The frequency of spraying the callus-promoting liquid and the rooting-promoting liquid was 6 times a day for 5 minutes each time.
In this example, in step one, the liquid for promoting callus formation comprises modified Hoagland nutrient solution and naphthylacetic acid, wherein the concentration of the modified Hoagland nutrient solution is 0.5 times that of the modified Hoagland nutrient solution, the concentration of the naphthylacetic acid is 100PPM, and the naphthylacetic acid (NAA) is added to facilitate the shoots to quickly form callus.
The branches are just cut until callus is formed, and the wound is susceptible to infection. In this embodiment, the liquid for promoting callus formation is further added with 10000 times of 90% carbendazim water dispersible granule and/or 3000 times of 1.8% abamectin water emulsion. The carbendazim water dispersible granule with 10000 times concentration and 90% content can effectively prevent and treat branch rot diseases, and the abamectin water emulsion with 3000 times concentration and 1.8% content can effectively prevent and treat root maggots and root-knot nematodes.
In the embodiment, the liquid for promoting rooting comprises modified Hoagland nutrient solution and indolebutyric acid, wherein the indolebutyric acid is 100PPM, and the indolebutyric acid (IBA) is favorable for rapid rooting of the callus parts.
TABLE 1 modified Hoagland nutrient solution formula (0.5 times standard nutrient solution concentration)
| Fertilizer | Content (mg/L) |
| Calcium nitrate tetrahydrate | 472.5 |
| Potassium nitrate | 253 |
| Ammonium nitrate | 40 |
| Potassium dihydrogen phosphate | 68 |
| Magnesium sulfate | 246.5 |
| Iron salt solution: ferrous sulfate heptahydrate | 1.39 |
| Disodium edetate (EDTA.Na) | 1.865 |
| Boric acid | 3.1 |
| Manganese sulfate | 11.15 |
| Zinc sulfate | 4.3 |
| Potassium iodide | 0.415 |
| Sodium molybdate | 0.125 |
| Copper sulfate | 0.013 |
| Cobalt chloride | 0.013 |
The water used to prepare the modified Hoagland nutrient solution was sterile purified water produced by an RO reverse osmosis water purifier. The concentration of the prepared nutrient solution is 0.5 time of that of the standard nutrient solution, and the pH value is 6.5.
The liquid for promoting the formation of the callus or the liquid for promoting the rooting is special nutrient solution and is prepared by sterile raw water, special nutrient solution formula fertilizer and special hormone, and water, nutrient and hormone conditions which are most beneficial to the rapid rooting of the callus generated at the lower part of the cuttage branch are created.
The rapid callus rooting method of the embodiment enables the lower parts of the cuttage branches to be in an aseptic and insect-pest-free environment, is beneficial to prevention and control of plant diseases and insect pests, and improves the survival rate of cuttage. Compared with the traditional soil or matrix cutting seedling culture, the rapid callus rooting method can save 50% of time for the formation of the callus; for fruit tree species which are not easy to root, such as jujube, apricot, peach, apple, bergamot pear, poplar and the like, the cuttage survival rate is improved from 0-50% to 80-100%.
The principle and the implementation mode of the present invention are explained by applying specific examples in the present specification, and the above descriptions of the examples are only used to help understanding the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (10)
1. A quick callus rooting system of cuttage branch which characterized in that: including cultivateing the room, cultivate indoor air temperature automatic regulating system, induced callus device and the controller of setting up, induced callus device is including the water tank, atomizer and the field planting pipe that connect gradually, air temperature automatic regulating system is used for control cultivate the temperature of room, the field planting pipe is used for the lower part of fixed cuttage branch, be provided with self-heating device in the water tank, be used for holding in the water tank and promote the liquid that forms callus or promote the liquid of takeing root, self-heating device is used for heating the liquid that holds in the water tank, the atomizer be used for with the liquid that holds in the water tank spouts in the field planting pipe, the atomizer opens and closes through time control switch control, the controller with air temperature automatic regulating system automatic heating device with the time control switch electricity is connected.
2. The system for rapid callus rooting of cutting shoots according to claim 1, wherein: and the field planting pipe is connected with a liquid discharge pipe, and the liquid discharge pipe is used for discharging liquid in the field planting pipe.
3. The system for rapid callus rooting of cutting shoots according to claim 1, wherein: the atomizer with the field planting pipe passes through the spray pipe intercommunication, the last a plurality of shower nozzles that are provided with of spray pipe, each the shower nozzle all is located in the field planting pipe.
4. The system for rapid callus rooting of cutting shoots according to claim 1, wherein: sponge blocks are arranged at the lower parts of the cuttage branches, and the sponge blocks are located in the planting pipes.
5. The system for rapid callus rooting of cutting shoots according to claim 1, wherein: the field planting pipe is horizontally arranged, a plurality of field planting holes are uniformly formed in the field planting pipe, and the field planting holes are used for enabling the lower portions of the cuttage branches to penetrate through.
6. The system for rapid callus rooting of cutting shoots according to claim 1, wherein: the culture room comprises a frame body, and a black and white film is arranged on the outer side of the frame body.
7. A rapid callus rooting method using the cutting shoot rapid callus rooting system according to any one of claims 1 to 6, characterized in that: the method comprises the following steps:
step one, opening an automatic air temperature regulating system to control the temperature in a culture chamber to be 15-18 ℃, opening an automatic heating device, and heating liquid which promotes callus formation in a water tank to 35-40 ℃;
spraying atomized liquid for promoting the lower part of the cuttage branch to form callus into the planting pipe, and ensuring the upper part of the cuttage branch to be under the condition of weak scattered light illumination at the temperature of 18-20 ℃; spraying for 2 minutes every 1h to enable the lower part of the cuttage branch to be in a constant-temperature high-humidity state with the temperature of 25-32 ℃ and the humidity of more than or equal to 90%, and further inducing the lower part of the cuttage branch to generate callus;
step three, after the lower part of the cuttage branch forms callus, replacing the liquid in the water tank with the liquid for promoting rooting, opening the automatic heating device, and heating the liquid in the water tank to 35-40 ℃;
spraying vaporous liquid for promoting rooting into the planting tube, ensuring the illumination condition until the lower part of the cuttage branch takes root, and spraying for 2min every 1h all day to ensure that the lower part of the cuttage branch is in a constant temperature and high humidity state, so as to promote the induction of callus to generate adventitious roots.
8. The rapid callus rooting method according to claim 7, characterized in that: in the first step, the liquid for promoting callus formation comprises a modified Hoagland nutrient solution and naphthylacetic acid, wherein the modified Hoagland nutrient solution comprises 472.5mg/L calcium nitrate tetrahydrate, 253mg/L potassium nitrate, 40mg/L ammonium nitrate, 68mg/L monopotassium phosphate, 246.5mg/L magnesium sulfate, 1.39mg/L ferrous sulfate heptahydrate, 1.865mg/L disodium oxalate, 3.1mg/L boric acid, 11.15mg/L manganese sulfate, 4.3mg/L zinc sulfate, 0.415mg/L potassium iodide, 0.125mg/L sodium molybdate, 0.013mg/L copper sulfate and 0.013mg/L cobalt chloride, and the naphthylacetic acid is 100PPM calculated according to the concentration of 0.5 times of the standard nutrient solution.
9. The rapid callus rooting method according to claim 8, characterized in that: the liquid for promoting callus formation is also added with 10000 times of 90% carbendazim water dispersible granules with concentration and/or 3000 times of 1.8% abamectin water emulsion with concentration, so that the occurrence of germ infection and insect damage can be effectively prevented and treated.
10. The rapid callus rooting method according to claim 8, characterized in that: the liquid for promoting rooting comprises the improved Hoagland nutrient solution and indole butyric acid, and the indole butyric acid is 100 PPM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243448.2A CN113796218A (en) | 2021-10-25 | 2021-10-25 | Rapid callus rooting system and rapid callus rooting method for cuttings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243448.2A CN113796218A (en) | 2021-10-25 | 2021-10-25 | Rapid callus rooting system and rapid callus rooting method for cuttings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113796218A true CN113796218A (en) | 2021-12-17 |
Family
ID=78898236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111243448.2A Pending CN113796218A (en) | 2021-10-25 | 2021-10-25 | Rapid callus rooting system and rapid callus rooting method for cuttings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113796218A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT202200025713A1 (en) * | 2022-12-15 | 2024-06-15 | Gimagi S R L Soc Agricola | PROCEDURE TO OBTAIN GRAFTED FRUIT PLANTS BY MULTIPLYING MICRO-GRAFTED PLANTS FROM SELF-ROOTED MATERIAL |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201355972Y (en) * | 2009-01-25 | 2009-12-09 | 张宗旺 | Nursery box of red date trees |
| CN106134959A (en) * | 2016-07-12 | 2016-11-23 | 四川农业大学 | A kind of Herba Sedi Aizoon mist training production method |
| CN106212236A (en) * | 2016-08-03 | 2016-12-14 | 江西省·中国科学院庐山植物园 | A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting |
| CN107032883A (en) * | 2017-04-21 | 2017-08-11 | 河南红枫种苗股份有限公司 | Take root nutrient solution and the golden armor mist training cuttage and seedling culture method of golden armor cuttings |
| CN110583467A (en) * | 2019-10-16 | 2019-12-20 | 广东圣之禾生物科技有限公司 | Automatic aeroponic rapid rooting and planting system and method for cuttage plants |
| CN110881482A (en) * | 2019-12-06 | 2020-03-17 | 广东红树林生态科技有限公司 | Rapid rooting liquid for mangrove plants and preparation method thereof |
| CN210695378U (en) * | 2019-10-16 | 2020-06-09 | 广东圣之禾生物科技有限公司 | Automatic aeroponics of cuttage class plant is taken root, is planted system fast |
| CN112704002A (en) * | 2021-01-22 | 2021-04-27 | 贵州大学 | Weining short-column camellia oleifera twig aeroponics rooting method |
| CN216163594U (en) * | 2021-10-25 | 2022-04-05 | 塔里木大学 | Rapid callus rooting system for cutting branches |
-
2021
- 2021-10-25 CN CN202111243448.2A patent/CN113796218A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201355972Y (en) * | 2009-01-25 | 2009-12-09 | 张宗旺 | Nursery box of red date trees |
| CN106134959A (en) * | 2016-07-12 | 2016-11-23 | 四川农业大学 | A kind of Herba Sedi Aizoon mist training production method |
| CN106212236A (en) * | 2016-08-03 | 2016-12-14 | 江西省·中国科学院庐山植物园 | A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting |
| CN107032883A (en) * | 2017-04-21 | 2017-08-11 | 河南红枫种苗股份有限公司 | Take root nutrient solution and the golden armor mist training cuttage and seedling culture method of golden armor cuttings |
| CN110583467A (en) * | 2019-10-16 | 2019-12-20 | 广东圣之禾生物科技有限公司 | Automatic aeroponic rapid rooting and planting system and method for cuttage plants |
| CN210695378U (en) * | 2019-10-16 | 2020-06-09 | 广东圣之禾生物科技有限公司 | Automatic aeroponics of cuttage class plant is taken root, is planted system fast |
| CN110881482A (en) * | 2019-12-06 | 2020-03-17 | 广东红树林生态科技有限公司 | Rapid rooting liquid for mangrove plants and preparation method thereof |
| CN112704002A (en) * | 2021-01-22 | 2021-04-27 | 贵州大学 | Weining short-column camellia oleifera twig aeroponics rooting method |
| CN216163594U (en) * | 2021-10-25 | 2022-04-05 | 塔里木大学 | Rapid callus rooting system for cutting branches |
Non-Patent Citations (2)
| Title |
|---|
| 吴少华: "园林苗圃学", 30 June 2004, 上海交通大学出版社, pages: 50 - 52 * |
| 江苏科技大学编: "中国栽桑养蚕专著系列 中国桑树栽培学", 31 December 2020, 上海科学技术出版社, pages: 310 - 311 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT202200025713A1 (en) * | 2022-12-15 | 2024-06-15 | Gimagi S R L Soc Agricola | PROCEDURE TO OBTAIN GRAFTED FRUIT PLANTS BY MULTIPLYING MICRO-GRAFTED PLANTS FROM SELF-ROOTED MATERIAL |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106170201B (en) | High density soilless plant growing systems and methods | |
| CN106386033A (en) | Seedling raising method for flue-cured tobacco | |
| CN109699477A (en) | A kind of cloud and mist cultivation cuttage breeding method of rosemary | |
| JP6779560B2 (en) | How to grow low temperature sensitive plants | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| CN100515175C (en) | Method for cultivation of arabidopsis | |
| CN103168690B (en) | Breeding method of Qi dioscorea opposita virus-free miniature seed beans | |
| CN113796218A (en) | Rapid callus rooting system and rapid callus rooting method for cuttings | |
| CN105724203A (en) | Method for stereoscopic mist-spray cultivation of hydroponic flowers | |
| CN110012755B (en) | A method and cultivation device for factory-based cultivation of rubber grass seedlings | |
| CN216163594U (en) | Rapid callus rooting system for cutting branches | |
| CN103999598A (en) | Cultivating method of oriental lily seedballs | |
| CN110199861A (en) | A kind of atomising cultivation method of celery | |
| CN110235626A (en) | A kind of method that numerous bergamot pear is expanded in scale | |
| CN105532294A (en) | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese | |
| CN105145299A (en) | Soilless seedling nursing method for muskmelon greenhouse | |
| CN105165356A (en) | Method for raising seedlings by cutting in fields | |
| RU2268571C1 (en) | Composition for maize seed pretreatment | |
| CN110692522A (en) | Tissue culture seedling method for Prunus cistana | |
| AU2020104022A4 (en) | A method to promote cuttage rooting on improved varieties of Carya illinoensis | |
| CN104585040A (en) | Method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis | |
| CN117136797A (en) | A method for raising asparagus bare root seedlings to prevent and control root rot | |
| CN104542210B (en) | A rapid propagation method for cranberry plants | |
| CN114847030A (en) | Light environment regulation and control method for regulating and controlling growth and development of roots of kombucha | |
| RU2715604C1 (en) | Method of producing healthier potato minitubers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |