CN113711728A - Seed treatment method for improving germination rate of hemerocallis fulva - Google Patents
Seed treatment method for improving germination rate of hemerocallis fulva Download PDFInfo
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- CN113711728A CN113711728A CN202111109654.4A CN202111109654A CN113711728A CN 113711728 A CN113711728 A CN 113711728A CN 202111109654 A CN202111109654 A CN 202111109654A CN 113711728 A CN113711728 A CN 113711728A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a seed treatment method for improving the germination rate of hemerocallis fulva, which comprises the following steps: s1: cleaning, namely rubbing and cleaning the surfaces of the seeds in clear water to remove the seeds floating on the water surface; s2: soaking, namely taking a clean beaker filled with distilled water, adding gibberellin into the beaker according to the amount of 2-3g/L of distilled water, uniformly stirring, then putting the seeds cleaned in S1 into the beaker, and soaking for 12-24 hours at 4 ℃; s3: peeling, namely taking out the seeds soaked in the S2, and peeling off the soaked and softened seed coats; s4: sowing; s5: and (3) culturing, namely placing the hole tray sowed in the S4 on a matched nonporous tray, and placing the whole tray in an environment with the temperature of 25-28 ℃ for culturing. The treatment method is simple and easy to operate, can obviously improve the germination rate of the seeds, greatly shortens the dormancy breaking time, and improves the seedling raising and breeding efficiency of the hemerocallis fulva seeds.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a seed treatment method for improving the germination rate of hemerocallis fulva.
Background
Hemerocallis (academic name) is a perennial herb with short rootstocks and stout, spindle-shaped fleshy roots. There are many other varieties of hemerocallis, such as "jin zhen", "daylily" and "forget-worry grass". The day lily is eaten mostly, becomes a delicious food of common people on a daily dining table, and has the effects of clearing heat, promoting urination, cooling blood and stopping bleeding, so that the day lily can be used for medicine. Hemerocallis are native to China, Siberia, Japan and southeast Asia, but the improvement is little although the hemerocallis are native to China, and the hemerocallis only propagate by sowing, so that the propagation rate is far from enough. The problems of low seed germination rate, weak germination potential, long consumed time, low seedling forming efficiency and the like exist in the conventional dormancy breaking technology and breeding and seedling raising. Therefore, there is an urgent need to develop a seed treatment method for increasing the germination rate of hemerocallis fulva to solve the above technical problems.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a seed treatment method for improving the germination rate of hemerocallis fulva, which utilizes the characteristic that gibberellin breaks seed dormancy, peels off seed coats for direct sowing, breaks seed coat barriers, and can efficiently promote seed germination. The treatment method is simple and easy to operate, can obviously improve the germination rate of the seeds, greatly shortens the dormancy breaking time, improves the seedling raising and breeding efficiency of the hemerocallis fulva seeds, has wide application prospect, and is favorable for popularization and application.
In order to achieve the above object, the present invention provides a seed treatment method for increasing germination rate of hemerocallis fulva, comprising the steps of:
s1: cleaning, namely rubbing and cleaning the surfaces of the seeds in clear water to remove the seeds floating on the water surface;
s2: soaking, namely taking a clean beaker filled with distilled water, adding gibberellin into the beaker according to the amount of 2-3g/L of distilled water, uniformly stirring, then putting the seeds cleaned in S1 into the beaker, and soaking for 12-24 hours at 4 ℃;
s3: peeling, namely taking out the seeds soaked in the S2, and peeling off the soaked and softened seed coats;
s4: sowing, namely, mixing a substrate by turfy soil and vermiculite, soaking the substrate by water, putting the mixture into a plug tray, compacting and flattening the mixture, sowing seeds with seeds of which S3 is peeled into the plug tray, burying the seeds and covering a layer of dry substrate on the upper layer after 5 seeds are sowed in each plug tray, wherein the sowing depth is 1cm, and the thickness of the covered dry substrate is consistent with that of the plug tray surface, and compacting and flattening the surface;
s5: and (3) culturing, namely placing the plug tray sowed by the S4 on a matched nonporous tray, and culturing the whole plug tray in an environment at 25-28 ℃.
Preferably, in the step S2, the gibberellin is mixed with the distilled water by stirring using a glass rod.
Preferably, in S3, the seed coat that has been macerated is removed by tweezers and the embryo of the seed cannot be damaged.
Preferably, in S4, the substrate after wetting is held by a hand to be clenched into a mass without water drops, and loosened into particles after being loosened.
Preferably, in S4, the seed with the peel off seed coat is sown in the plug using tweezers.
Preferably, in the S4, the volume ratio of the peat soil to the vermiculite in the matrix is 3: 1.
Preferably, in S4, the size of the plug is 8cm × 8 cm.
Preferably, in the step S5, after sowing, the temperature and humidity of the substrate are kept, a large amount of water cannot be accumulated in the tray, the surface of the substrate cannot be dried too much, and the temperature cannot be higher than 27 ℃.
The seed treatment method for improving the germination rate of the hemerocallis fulva has the following beneficial effects.
1. The invention utilizes the characteristic that gibberellin breaks seed dormancy, peels off seed coats for direct sowing, breaks seed coat barriers, and can efficiently promote seed germination. The treatment method is simple and easy to operate, can obviously improve the germination rate of the seeds, greatly shortens the dormancy breaking time, improves the seedling raising and breeding efficiency of the hemerocallis fulva seeds, has wide application prospect, and is favorable for popularization and application.
2. Stripping off the seed coat before sowing the hemerocallis fulva can improve the moisture permeability and the gas exchange speed, prevent dormancy caused by seed swell due to the seed coat, and can improve the hemerocallis fulva germination rate due to the fact that the hemerocallis fulva seed coat contains substances for inhibiting seed germination.
Detailed Description
The present invention will be further described with reference to specific examples to assist understanding of the invention.
The invention provides a seed treatment method for improving the germination rate of hemerocallis fulva, which comprises the following steps:
s1: cleaning, namely rubbing and cleaning the surfaces of the seeds in clear water to remove the seeds floating on the water surface;
s2: soaking, namely taking a clean beaker filled with distilled water, adding gibberellin into the beaker according to the amount of 2-3g/L of distilled water, uniformly stirring the gibberellin and the distilled water by using a glass rod, then putting the seeds cleaned by S1 into the beaker, and soaking for 12-24 hours at 4 ℃;
s3: peeling, taking out the seeds soaked in the S2, and peeling off the seed coats which are soaked soft by using tweezers, wherein embryos of the seeds cannot be damaged.
S4: sowing, wherein the matrix is prepared by mixing turfy soil and vermiculite according to the volume ratio of 3:1, the turfy soil and the vermiculite are soaked in water and then are placed into a plug tray with the size of 8cm multiplied by 8cm to be compacted and flattened, the humidity of the soaked matrix is preferably that the matrix is held by hands, the matrix is tightly clenched and then is agglomerated without water drops, and the matrix is loosened to be particles. Sowing seeds with the seed coats peeled off in the S3 cavities by using tweezers, burying the seeds and covering a layer of dry matrix on the upper layer after 5 seeds are sowed in each cavity, wherein the sowing depth is 1cm, the thickness of the covered dry matrix is consistent with that of the cavity surface, and the surface is compacted and flattened;
s5: and (3) culturing, namely placing the plug tray sowed in the S4 on a matched nonporous tray, culturing the whole plug tray in an environment of 25-28 ℃, paying attention to temperature and keeping humidity of the substrate after sowing, wherein a large amount of water cannot be accumulated in the tray, the surface of the substrate cannot be dried excessively, and the temperature cannot be higher than 27 ℃.
And (3) comparison test:
by adopting the seed treatment method for improving the germination rate of the hemerocallis fulva, 360 full seeds are selected by taking the seeds of Sterla as materials, and gibberellin treatment is carried out to design 4 concentration gradients (C) and 3 time gradients (T), wherein C is 0g/L, 2g/L, 2.5g/L and 3g/L, and T is 12 h, 18 h and 24h respectively. Of these, 0g/L (without addition of gibberellin) was used as a control group, and the remaining 3 concentration gradients were used as gibberellin-treated groups, for a total of 12 treatment protocols, each treatment protocol using 30 seeds, which were repeated 3 times. The test results are as follows: the germination rates of 0g/L (without adding gibberellin) of the control group in the seed germination rate are 25% averagely after being soaked for 12 h, 18 h and 24h at 4 ℃, and the germination rates of the 0g/L, 18 h and 24h of the gibberellin treated group in the seed germination rate are 50% averagely after being soaked for 12 h, 18 h and 24h at 4 ℃ in the gibberellin treated group in the gibberellin solution. Therefore, the germination rate of the hemerocallis fulva seeds is remarkably improved after the hemerocallis fulva seeds are treated by the method.
The invention utilizes the characteristic that gibberellin breaks seed dormancy, peels off seed coats for direct sowing, breaks seed coat barriers, and can efficiently promote seed germination. The processing method is simple and easy to operate, can obviously improve the germination rate of seeds, greatly shorten the dormancy breaking time, improve the seedling raising and breeding efficiency of the hemerocallis fulva seeds, can improve the moisture permeability and the gas exchange speed by peeling off the seed coat before the hemerocallis fulva seeds are sown, prevents dormancy caused by seed imbibition inhibition of the seed coat, and can improve the germination rate of the hemerocallis fulva seeds by peeling off the hemerocallis fulva seed coat.
The inventive concept is explained in detail herein using specific examples, which are given only to aid in understanding the core concepts of the invention. It should be understood that any obvious modifications, equivalents and other improvements made by those skilled in the art without departing from the spirit of the present invention are included in the scope of the present invention.
Claims (8)
1. A seed treatment method for improving the germination rate of hemerocallis fulva is characterized by comprising the following steps:
s1: cleaning, namely rubbing and cleaning the surfaces of the seeds in clear water to remove the seeds floating on the water surface;
s2: soaking, namely taking a clean beaker filled with distilled water, adding gibberellin into the beaker according to the amount of 2-3g/L of distilled water, uniformly stirring, then putting the seeds cleaned in S1 into the beaker, and soaking for 12-24 hours at 4 ℃;
s3: peeling, namely taking out the seeds soaked in the S2, and peeling off the soaked and softened seed coats;
s4: sowing, namely, mixing a substrate by turfy soil and vermiculite, soaking the substrate by water, putting the mixture into a plug tray, compacting and flattening the mixture, sowing seeds with seeds of which S3 is peeled into the plug tray, burying the seeds and covering a layer of dry substrate on the upper layer after 5 seeds are sowed in each plug tray, wherein the sowing depth is 1cm, and the thickness of the covered dry substrate is consistent with that of the plug tray surface, and compacting and flattening the surface;
s5: and (3) culturing, namely placing the plug tray sowed by the S4 on a matched nonporous tray, and culturing the whole plug tray in an environment at 25-28 ℃.
2. The method for treating seeds with increased germination rate of daylily according to claim 1, wherein in S2, gibberellin is mixed with distilled water by stirring with a glass rod.
3. The method of claim 1, wherein in step S3, the seed coat that has been macerated is removed with tweezers, and the embryo of the seed cannot be destroyed.
4. The method as claimed in claim 1, wherein in step S4, the moisture content of the wetted substrate is selected to be grasped by a hand, to be pinched, to be held in a cluster without water drops, and to be loosened to be particles.
5. The method of claim 1, wherein in step S4, tweezers are used to sow the seeds with peel off the seed coat into the plug.
6. The method of claim 1, wherein in step S4, the volume ratio of the turfy soil to the vermiculite in the medium is 3: 1.
7. The method of claim 1, wherein in step S4, the tray size is 8cm x 8 cm.
8. The method of claim 1, wherein in step S5, the temperature and humidity of the substrate are kept after seeding, the tray can not have a lot of water, the surface of the substrate can not be dried too much, and the temperature can not be higher than 27 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111109654.4A CN113711728A (en) | 2021-09-22 | 2021-09-22 | Seed treatment method for improving germination rate of hemerocallis fulva |
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| CN202111109654.4A CN113711728A (en) | 2021-09-22 | 2021-09-22 | Seed treatment method for improving germination rate of hemerocallis fulva |
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| CN113711728A true CN113711728A (en) | 2021-11-30 |
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| CN202111109654.4A Withdrawn CN113711728A (en) | 2021-09-22 | 2021-09-22 | Seed treatment method for improving germination rate of hemerocallis fulva |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005012507A1 (en) * | 2003-07-25 | 2005-02-10 | The University Of Melbourne | Production of plant secondary metabolites using adsorption and elicitation in cell suspension culture |
| CN112088730A (en) * | 2020-08-18 | 2020-12-18 | 云南吉成园林科技股份有限公司 | Hemerocallis fulva tissue culture seedling domestication method |
-
2021
- 2021-09-22 CN CN202111109654.4A patent/CN113711728A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005012507A1 (en) * | 2003-07-25 | 2005-02-10 | The University Of Melbourne | Production of plant secondary metabolites using adsorption and elicitation in cell suspension culture |
| CN112088730A (en) * | 2020-08-18 | 2020-12-18 | 云南吉成园林科技股份有限公司 | Hemerocallis fulva tissue culture seedling domestication method |
Non-Patent Citations (3)
| Title |
|---|
| CALEB D. MCMANHAN ET AL.: ""The effects of Gibberellic Acid on seed germination of daylilies"", 《SOUTH CAROLINA JUNIOR ACADEMY OF SCIENCES》 * |
| 何琦 等: ""不同处理下萱草种子萌发研究"", 《种子》 * |
| 褚焕宁: ""萱草属植物快繁体系构建及杂种胚拯救研究"", 《中国优秀硕士学位论文 农业科技辑》 * |
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