CN113588748A - Full blood triggered electrochemical test paper - Google Patents
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a full blood triggered electrochemical test paper which comprises a PET (polyethylene terephthalate) substrate, a washtrough white glue layer, a hydrophilic film layer and a cover sheet layer which are sequentially arranged, wherein an electrode layer is attached to the PET substrate and comprises a reaction area, a working electrode, a counter electrode, a full blood electrode and pins, the reaction area is provided with one end of the PET substrate, the reaction area is provided with an enzyme layer, the working electrode, the counter electrode and the full blood electrode are sequentially arranged on the reaction area respectively, the full blood electrode is positioned at one end far away from a sampling port of the reaction area, the pins are used for correspondingly connecting the working electrode, the counter electrode and the full blood electrode, and the pins are arranged at the other end of the PET substrate and are used for connecting a detection instrument. Can be used for monitoring the contents of cholesterol, blood sugar, ketone bodies, uric acid and the like in a human body and providing technical support for early detection and prevention of diseases.
Description
Technical Field
The invention belongs to the technical field of electrochemical test paper, and particularly relates to full blood triggered electrochemical test paper.
Background
With the improvement of living standard of people, the eating habits are continuously changed, the work pressure causes the irregularity of daily work and rest, and the risk of suffering from chronic diseases is increased day by day, so that the daily monitoring of the body can be effectively found and can be used for preventing as soon as possible, and the daily monitoring is mainly reflected in several aspects of cholesterol, blood sugar, ketone bodies and uric acid at present.
Cholesterol is a derivative of cyclopentaphenanthrene synthesized from acetyl coenzyme a by condensation reaction of isoprene units, is an important raw material for synthesizing physiologically active substances such as adrenocortical hormone, sex hormone, bile acid and vitamin D, and is also a main component constituting cell membranes, and its serum concentration can be used as an index of lipid metabolism. At present, the determination of cholesterol is mainly used for cardiovascular disease risk analysis, and when the cholesterol is increased, the cholesterol is commonly found in atherosclerosis, primary hyperlipidemia, common bile duct blockage and the like; when cholesterol is low, it is often seen in hypoalbuminemia, anemia, sepsis, malnutrition, etc.
Glucose in the blood is called blood glucose (Glu). Glucose is an important constituent of the human body and also an important source of energy. The monitoring of blood sugar can screen the diabetic better and control the blood sugar change of the diabetic better, all has important guiding significance to the law of life, activity, motion, diet and reasonable medication, and can help the patient find problems at any time and get to the hospital to seek medical advice in time.
Ketone bodies are intermediates in the normal catabolism of fatty acids in the liver, including beta-hydroxybutyrate, acetoacetate and acetone. Because the diabetes patient can not normally utilize glucose oxidation energy supply, the decomposition of fat in vivo is increased, the metabolism of fatty acid in liver is correspondingly vigorous, and a large amount of ketone bodies in vivo are accumulated, thereby causing ketosis and ketoacidosis. Moreover, studies have shown that diabetic patients have a significant increase in the concentration of ketone bodies in their blood before the blood glucose level increases, and therefore, determining ketone body concentrations is of interest for early warning of diabetes.
Uric acid is a final product of purine metabolism in a human body, is mainly formed by decomposing nucleic acid decomposed by cell metabolism, other purine compounds and purine in food through the action of enzyme, has weak acidity and reducibility, and has an important effect on protecting the human body from being damaged by free radicals. Under normal conditions, the rate of production and excretion of uric acid in human bodies is relatively constant, and the change of the uric acid content in body fluid can fully reflect the functional conditions of metabolism, immunity and the like of the human bodies. When the concentration of uric acid in blood is too high, a series of diseases such as diabetes, hypertension, gout, heart failure and the like can be caused, so that the detection of the content of uric acid in human bodies is particularly important.
At present, when indexes such as cholesterol, blood sugar, ketone body and uric acid in a human body are detected, a large-scale biochemical analyzer and professional personnel are needed for operation, so that the detection time and cost are increased. Based on the situation, the electrochemical test paper which is simple and easy to use is designed for quickly detecting the contents of cholesterol, blood sugar, ketone bodies, uric acid and the like in the blood of a human body, and has great significance.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the full blood triggered electrochemical test paper which is used for monitoring the contents of cholesterol, blood sugar, ketone bodies, uric acid and the like in a human body and provides technical support for early discovery and prevention of diseases.
In order to achieve the technical purpose, the invention specifically adopts the following technical scheme:
the utility model provides an electrochemistry test paper that full blood triggered, is including the PET substrate, the scouring bath white glue film, hydrophilic rete and the cover plate layer that set gradually, it has the electrode layer to adhere to on the PET substrate, and the electrode layer includes reaction zone, working electrode, counter electrode, full blood electrode and pin, the reaction zone sets up the one end of PET substrate, is provided with the enzyme layer on the reaction zone, working electrode, counter electrode and full blood electrode set gradually respectively on the reaction zone, and full blood electrode is located the one end of keeping away from the reaction zone sample connection, the pin is used for corresponding connection working electrode, counter electrode and full blood electrode, the pin sets up the other end at the PET substrate and is used for connecting detecting instrument.
And the electrode layer is printed on the PET sheet by conductive silver paste, conductive carbon paste and insulating ink in a screen printing mode. The conductive carbon paste is used for preparing a working electrode, a counter electrode, a full blood electrode and pins, the conductive silver paste is used for preparing leads for connecting the working electrode, the counter electrode, the full blood electrode and the pins, and the insulating ink is attached to a circuit and leaks out of a reaction area and the pins.
The notching white glue layer is pasted on the electrode layer, a first through groove is formed in one end of the notching white glue layer, and the first through groove is arranged corresponding to the reaction area.
Hydrophilic rete adhere to on the white glue film layer of dashing groove, be provided with the exhaust hole on last hydrophilic rete, the exhaust hole corresponds the one end that sets up the reaction zone sample connection of keeping away from in the reaction zone, exhaust hole, enzyme layer and hydrophilic rete constitute siphon structure, help the blood specimen to be covered with the reaction zone.
The cover sheet layer is attached to the hydrophilic film layer, the cover sheet layer is provided with a second through groove, the second through groove corresponds to the first through groove, and the vent hole of the hydrophilic film layer is leaked.
The invention has the beneficial effects that:
1) the electrochemical test paper can quickly detect the contents of cholesterol, blood sugar, ketone bodies, uric acid and the like in the blood of a human body;
2) the full blood electrode is added, when the reaction area is filled with blood, the full blood trigger electrode is conducted to start detection, if the reaction area is not filled with blood, the full blood trigger electrode is not conducted, and the full blood trigger electrode is prompted to be not filled with blood, so that the risk of misleading by an inaccurate test result under the condition that the blood is not filled and is not perceived in the test process can be completely avoided;
3) the silver electrode and the carbon electrode are used for manufacturing the test paper, and most of the used reagents are stable and cheap, so that the production cost is greatly reduced.
Drawings
FIG. 1 is a schematic diagram of a seal structure of the electrochemical test paper of the present invention;
FIG. 2 is a schematic diagram of an electrode layer structure of the electrochemical test strip of the present invention;
FIG. 3 is a schematic view of a structure of a notching white glue layer of the electrochemical test paper of the present invention;
FIG. 4 is a schematic diagram of the structure of the hydrophilic membrane layer of the electrochemical test paper of the present invention;
FIG. 5 is a schematic diagram of the structure of the cover sheet layer of the electrochemical test strip of the present invention;
FIG. 6 is a schematic diagram of the electrode layer structure of the electrochemical HCT compensation test paper of the present invention;
FIG. 7 is a schematic view of the structure of the notching white glue layer of the electrochemical HCT compensation test paper of the present invention;
FIG. 8 is a schematic diagram of a CODE structure of electrochemical test paper CODE;
in the figure, 1: PET substrate, 2: full blood electrode, 3: working electrode, 4: reaction zone, 5: counter electrode, 6-8, 18-19: pin, 9: notching white glue layer, 10: a first through groove; 11: a hydrophilic film layer; 12: an exhaust hole; 13: a cover sheet layer; 14: a second through groove; 15: a sampling port; 16: a HCT working electrode 16; 17: a HCT counter electrode; 20: CODE.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in figure 1, the invention provides a full blood triggered electrochemical test paper, which comprises a PET substrate 1, a notching white glue layer 9, a hydrophilic film layer 11 and a cover sheet layer 13 which are sequentially arranged from bottom to top, wherein an electrode layer is attached to the PET substrate 1.
As shown in fig. 2, an electrode layer is attached to a PET substrate 1, and the electrode layer is printed on a PET sheet by screen printing using conductive silver paste, conductive carbon paste, and insulating ink. The electrode layer includes reaction zone 4, working electrode 3, counter electrode 5, full blood electrode 2 and a plurality of pin (6 ~ 8), and reaction zone 4 is located the top center of PET substrate 1, is provided with the enzyme layer on the reaction zone 4, has set gradually working electrode 3, counter electrode 5 and full blood electrode 2 on the reaction zone 4 respectively, and wherein full blood electrode 2 is located the least significant end of reaction zone 4 for whether detection reaction zone 4 is covered with blood. Three pins (6-8) are respectively arranged at the bottom of the PET substrate 1, and the three pins (6-8) are respectively and correspondingly connected with the working electrode 3, the counter electrode 5 and the full blood electrode 2.
Specifically, the conductive carbon paste is used for preparing a working electrode 3, a counter electrode 5, a full blood electrode 2 and pins (6-8), the conductive silver paste is used for preparing leads for connecting the working electrode 3, the counter electrode 5, the full blood electrode 2 and the pins (6-8), wherein the working electrode 3 and the pin 8, the counter electrode 5 and the pin 7, and the full blood electrode 2 and the pin 6 are correspondingly connected, and the insulating ink is attached to a circuit and leaks out of the reaction area 4 and the pins (6-8).
As shown in fig. 3, a first through groove 10 is arranged in the center of the top of the punched-out prepreg layer 9, the size and position of the first through groove 10 correspond to the reaction region 4 of the electrode layer, and a sampling port 15 is formed by the first through groove 10.
As shown in fig. 4, the vent 12 is disposed on the upper hydrophilic film 11, the vent 12 is located at the bottom of the first through groove 10, and the vent 12, the enzyme layer and the hydrophilic film 11 form a siphon structure to help the blood sample to spread over the whole reaction area 4.
As shown in fig. 5, the cover sheet layer 13 is provided with a second through groove 14, and the second through groove 14 is corresponding to the first through groove 10 and leaks out of the vent 12 of the hydrophilic film layer 11 for siphoning.
In another embodiment of the present invention, an electrochemical test strip with HCT compensation function is further provided, as shown in fig. 6, in the structure of the test strip, the electrode layer further includes a HCT working electrode 16, a HCT counter electrode 17, and corresponding pins (18-19).
As shown in fig. 7, the HCT working electrode 16 and HCT counter electrode 17 were divided into the working electrode 3, counter electrode 5, and full blood electrode 2 regions by the washout gel layer 9, and the HCT working electrode 16 and HCT counter electrode 17 were measured by measuring the impedance of blood without providing an enzyme layer in the reaction region 4 corresponding to the HCT working electrode 16 and HCT counter electrode 17 and without the presence of a reactant.
In the independent HCT test area, reactants do not exist on the HCT electrode, so that the HCT test cannot be interfered, the HCT test result is more accurate, HCT compensation can be correctly carried out, and the final blood sugar test result is more accurate.
In another embodiment of the present invention, as shown in fig. 8, the bottom of the electrochemical test paper of the present invention is further provided with a CODE 20 for indicating a linear relationship between a reaction current and a concentration of the test paper, the test paper does not need to be matched with a CODE card when performing a test, and a designed CODE electrode is combined according to different arrangements to be identified by an instrument by a barcode, so that a cost for manufacturing the CODE card is saved, a test step is simpler, and a user can perform an accurate test without depending on the CODE card.
Example 1
A manufacturing method of electrochemical uric acid test paper comprises the following steps:
1) taking a PET substrate, and printing conductive silver paste, conductive carbon paste and insulating ink on the PET substrate, wherein the electrode consists of a working electrode, a counter electrode and a full blood trigger electrode. The working electrode and the counter electrode measure the current value generated by the reaction of uric acid in blood and the enzyme layer of the reaction area so as to calculate the concentration; the full blood electrode judges whether the blood is full or not by detecting the current after the blood is sucked, and the non-full blood is not contacted with the full blood trigger electrode, so that the non-full blood is prompted to be full; and covering the electrode with insulating ink, and exposing a reaction area and an electrode pin with a certain area to obtain the PET uric acid test paper substrate.
2) After the treatment fluid containing polyvinyl alcohol is prepared, the reaction area is subjected to hydrophilic treatment by using the treatment fluid, and then the reaction area is dried in a drying tunnel. The drying temperature of the treatment liquid is 50 ℃, and the time is 4 min.
3) Loading the prepared reaction liquid medicine into a reaction area, and drying in a drying tunnel at 50 ℃ for 20-25 min. The reaction liquid medicine is prepared according to the proportion that a phosphate buffer solution with the pH value of 6.7 accounts for 87 percent, an electronic mediator potassium ferricyanide accounts for 5 percent, a film forming agent K90 accounts for 5 percent, a surfactant Tween 20 accounts for 1 percent and Triton X-100 accounts for 2 percent.
4) After the liquid medicine in the reaction area is dried, a notching white glue layer is adhered, then a hydrophilic film is adhered, and finally a cover plate is adhered. The material of the notching white glue layer is Sowess WH234343 type double-sided adhesive tape, the hydrophilic membrane is subjected to single-sided hydrophilic treatment, the 3M 9901P type is used, and the material of the cover plate is synthetic paper.
The reaction mechanism of the scheme is that uric acid in blood reacts with potassium ferricyanide to generate potassium ferrocyanide, then the potassium ferrocyanide is oxidized into potassium ferricyanide under the voltage of +400mV to generate current, the magnitude of the current value is in direct proportion to the concentration of uric acid, and the concentration of uric acid in blood can be converted according to the magnitude of the detected current value; the full blood electrode is arranged at the innermost side of the reaction area, full blood is sucked and contacts with the full blood electrode to conduct, the full blood is sucked and then 400mV is driven to pressurize, and if the blood sucking quantity is insufficient, the full blood is not conducted, and the full blood is not sucked.
Example 2
The method for manufacturing the electrochemical ketone body test paper comprises the following steps:
1) taking a PET substrate, and printing conductive silver paste, conductive carbon paste and insulating ink on the PET substrate, wherein the electrode consists of a working electrode, a counter electrode and a full blood trigger electrode. The working electrode and the counter electrode are used for measuring the current value generated by the reaction of the ketone body in the blood and the enzyme layer in the reaction area so as to calculate the concentration; the full blood electrode judges whether the blood is full or not by detecting the current after the blood is sucked, and the non-full blood is not contacted with the full blood trigger electrode, so that the non-full blood is prompted to be full; and covering the electrode with insulating ink, and exposing a reaction area and electrode pins with a certain area to obtain the PET ketone body test paper substrate.
2) After the treatment fluid containing polyvinyl alcohol is prepared, the reaction area is subjected to hydrophilic treatment by using the treatment fluid, and then the reaction area is dried in a drying tunnel. The drying temperature of the treatment liquid is 50 ℃, and the time is 4 min.
3) Loading the prepared reaction liquid medicine into a reaction area, and drying in a drying tunnel at 50 ℃ for 20-25 min. The reaction liquid medicine is prepared according to the proportion that a phosphate buffer solution with the pH of 7.3 accounts for 90 percent, beta-hydroxybutyrate dehydrogenase accounts for 1.5 percent, Nicotinamide Adenine Dinucleotide (NAD) accounts for 1 percent, diaphorase accounts for 1.5 percent, an electron mediator accounts for 1 percent, an enzyme protective agent BSA and cane sugar respectively account for 2 percent and 0.5 percent, sodium chloride accounts for 0.5 percent, and a surfactant Tween 20 and polypropylene glycol respectively account for 1 percent.
4) After the liquid medicine in the reaction area is dried, a notching white glue layer is adhered, then a hydrophilic film is adhered, and finally a cover plate is adhered. The material of the notching white glue layer is Sowess WH234343 type double-sided adhesive tape, the hydrophilic membrane is subjected to single-sided hydrophilic treatment, the 3M 9901P type is used, and the material of the cover plate is synthetic paper.
The reaction mechanism of the scheme is that beta-hydroxybutyric acid in blood reacts with NAD under the action of beta-hydroxybutyric acid dehydrogenase to generate NADH, then the NADH reacts with an electron mediator under the action of diaphorase to generate a sub-form of the electron mediator, then the sub-form of the electron mediator is oxidized to generate current under the voltage of +400mV, the magnitude of the current is in direct proportion to the beta-hydroxybutyric acid in the blood, namely the current is also in direct proportion to ketone bodies in the blood, and the concentration of the ketone bodies in the blood can be calculated according to the magnitude of the detected current value; the full blood electrode is arranged at the innermost side of the reaction area, full blood is sucked and contacts with the full blood electrode to conduct, the full blood is sucked and then 400mV is driven to pressurize, and if the blood sucking quantity is insufficient, the full blood is not conducted, and the full blood is not sucked.
Example 3
A manufacturing method of electrochemical blood sugar test paper comprises the following steps:
1) taking a PET substrate, printing conductive silver paste, conductive carbon paste and insulating ink on the PET substrate, wherein the electrodes consist of a working electrode, a counter electrode (formed by silk-screen printing of carbon paste), a full blood trigger electrode (formed by silk-screen printing of carbon paste) and a row of CODE electrodes (formed by silk-screen printing of carbon paste), one group of HCT electrodes exist in an independent reaction area, HC is measured by measuring the impedance of blood, the working electrode and the counter electrode measure the current value generated by the reaction of glucose in the blood and liquid medicine in the reaction area so as to calculate the concentration, the full blood electrode judges whether the blood is fully absorbed or not by detecting the current after the blood is absorbed, the full blood is not contacted with the full blood trigger electrode, the full blood is not fully absorbed, the CODE electrodes distinguish CODE CODEs by the arrangement and combination of mutual short circuits, during the test, a CODE card does not need to be inserted for calibration and then the test, covering the electrode with insulating ink, and exposing a reaction region and electrode pins with a certain area to obtain a PET blood glucose test paper substrate;
2) after the treatment fluid containing polyvinyl alcohol is prepared, the reaction area is subjected to hydrophilic treatment by using the treatment fluid, and then the reaction area is dried in a drying tunnel. The drying temperature of the treatment liquid is 50 ℃, and the time is 4 min.
3) Loading the prepared reaction liquid medicine containing glucose dehydrogenase and potassium ferricyanide into the reaction area, and drying in a drying tunnel; the drying temperature is 50 ℃ and the drying time is 5 min. The enzyme solution is prepared according to the proportion that a phosphate buffer solution with the pH value of 6.8 accounts for 86 percent, a surfactant Tween 20 accounts for 1 percent, hydroxyethyl cellulose accounts for 2.5 percent, glucose dehydrogenase accounts for 3 percent, and potassium ferricyanide accounts for 7.5 percent.
4) After the liquid medicine in the reaction area is dried, a notching white glue layer is adhered, then a hydrophilic film is adhered, and finally a cover plate is adhered. The material of the notching white glue layer is Sowess WH234343 type double-sided adhesive tape, the hydrophilic membrane is subjected to single-sided hydrophilic treatment, the 3M 9901P type is used, and the material of the cover plate is synthetic paper.
The reaction mechanism of the scheme is that glucose in blood reacts with potassium ferricyanide under the action of glucose dehydrogenase, the potassium ferricyanide is directly reduced into potassium ferrocyanide, the potassium ferrocyanide is oxidized into potassium ferricyanide under the voltage of +400mV, current is generated, and the linear relation between the glucose concentration and the current is selected and determined through the permutation and combination of the code electrodes, so that the glucose concentration is obtained; HCT test is to measure the impedance of blood by HCT electrode to obtain HCT, and HCT compensation is carried out; the whole test process does not need to independently insert a CODE card for calibration, and the most appropriate CODE is determined and selected through the arrangement and combination of CODE electrodes, so that the bar CODE-free function is realized; the full blood electrode is arranged at the innermost side of the reaction area, full blood is sucked and contacts with the full blood electrode to conduct, the full blood is sucked and then 400mV is driven to pressurize, and if the blood sucking quantity is insufficient, the full blood is not conducted, and the full blood is not sucked.
Example 4
The method for manufacturing the electrochemical total cholesterol test paper comprises the following steps:
1) taking a PET substrate, and printing conductive silver paste, conductive carbon paste and insulating ink on the PET substrate, wherein the electrode consists of a working electrode, a counter electrode and a full blood trigger electrode. The working electrode and the counter electrode measure the current value generated by the reaction of total cholesterol in blood and the enzyme layer in the reaction area so as to calculate the concentration; the full blood electrode judges whether the blood is full or not by detecting the current after the blood is sucked, and the non-full blood is not contacted with the full blood trigger electrode, so that the non-full blood is prompted to be full; and covering the electrode with insulating ink, and exposing a reaction region and electrode pins with a certain area to obtain the PET cholesterol test paper substrate.
2) After the treatment fluid containing polyvinyl alcohol is prepared, the reaction area is subjected to hydrophilic treatment by using the treatment fluid, and then the reaction area is dried in a drying tunnel. The drying temperature of the treatment liquid is 50 ℃, and the time is 4 min.
3) Loading the prepared enzyme solution into a reaction zone, and drying in a drying tunnel at 50 ℃ for 20-25 min. The enzyme solution is prepared according to the proportion that MOPS buffer solution with the pH of 7.3 accounts for 87 percent, cholesterol esterase accounts for 2 percent, cholesterol oxidase accounts for 2 percent, peroxidase accounts for 1 percent, surfactant Tween 20, polypropylene glycol and film-forming agent K90 respectively account for 1 percent, and electron mediator potassium ferrocyanide accounts for 5 percent.
4) After the liquid medicine in the reaction area is dried, a notching white glue layer is adhered, then a hydrophilic film is adhered, and finally a cover plate is adhered. The material of the notching white glue layer is Sowess WH234343 type double-sided adhesive tape, the hydrophilic membrane is subjected to single-sided hydrophilic treatment, the 3M 9901P type is used, and the material of the cover plate is synthetic paper.
The reaction mechanism of the scheme is that total cholesterol ester in blood is hydrolyzed into free cholesterol under the action of cholesterol esterase, the free cholesterol is decomposed to generate hydrogen peroxide under the action of cholesterol oxidase, the hydrogen peroxide reacts with potassium ferrocyanide under the action of peroxidase to generate potassium ferricyanide, then the potassium ferricyanide is reduced into the potassium ferrocyanide under the voltage of +400mV to generate current, the magnitude of the current is in direct proportion to the concentration of the cholesterol in the blood, and the concentration of the total cholesterol in the blood can be converted according to the magnitude of the generated current; the full blood electrode is arranged at the innermost side of the reaction area, full blood is sucked and contacts with the full blood electrode to conduct, the full blood is sucked and then 400mV is driven to pressurize, and if the blood sucking quantity is insufficient, the full blood is not conducted, and the full blood is not sucked.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. The utility model provides an electrochemistry test paper that full blood triggered, is including the PET substrate, the dashing groove white glue film, hydrophilic rete and the cover plate layer that set gradually, its characterized in that, it has the electrode layer to adhere to on the PET substrate, the electrode layer includes reaction zone, working electrode, counter electrode, full blood electrode and pin, the reaction zone sets up the one end of PET substrate, is provided with the enzyme layer on the reaction zone, working electrode, counter electrode and full blood electrode set gradually respectively on the reaction zone, and full blood electrode is located the one end of keeping away from the reaction zone sample connection, the pin is used for corresponding connection working electrode, counter electrode and full blood electrode, the pin sets up the other end at the PET substrate and is used for connecting detecting instrument.
2. The full blood triggered electrochemical test paper as claimed in claim 1, wherein the electrode layer is printed on the PET sheet by screen printing with conductive silver paste, conductive carbon paste and insulating ink.
3. The full blood triggered electrochemical test paper as claimed in claim 1, wherein a first through slot is disposed at one end of the notched albumin layer, and the first through slot is disposed corresponding to the reaction region.
4. The full blood triggered electrochemical test paper as claimed in claim 3, wherein the upper hydrophilic membrane layer is provided with an air vent, the air vent is correspondingly arranged at one end of the reaction zone away from the reaction zone sampling port, and the air vent, the enzyme layer and the hydrophilic membrane layer form a siphon structure.
5. The full blood triggered electrochemical test strip according to claim 4 wherein said cover sheet has a second through slot formed therein, said second through slot corresponding to said first through slot.
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