CN113367254B - Composition for feeding ladybug, preparation method thereof and feed - Google Patents

Abstract

The invention provides a composition for feeding Coccinella septempuncata and its preparation method and feed. The composition includes the following components by mass: 500 to 1000 parts of aphid treatment, 1 to 4 parts of β-carotene and guacamole. Juvenile hormone 0.000001~0.00001 parts etc. The feed composition provided by the invention includes an aphid treatment, juvenile hormone and β-carotene. The aphid treatment has comprehensive basic nutrition, the juvenile hormone promotes the development of adult ovaries, and β-carotene improves insect survival rate and protects follicles and According to the characteristics of the uterine cells, there is no significant difference in the egg-laying amount and hatching rate between the ladybugs raised using the composition and the ladybugs fed using conventional bean aphids, and the feed is easy to preserve and easy to use. This patent can reduce the overall demand for aphids by about 20%, save space by more than 50%, and reduce labor requirements by more than 30%.

Description

A composition for feeding Coccinella septempunctata and its preparation method and feed

Technical field

The invention belongs to the technical field of feed, and specifically relates to a composition for feeding Coccinella septempunctata, its preparation method and feed.

Background technique

Aphids are important pests on many crops. Due to the wide variety of aphids, their strong diffusion ability, short development period, and rapid reproduction, they can feed directly and spread plant viruses, causing plant nutrition to deteriorate or even die, seriously affecting crops. output and quality

The seven-star ladybug is the dominant natural enemy of aphids in the family Coleoptera, Coccinellidae. However, due to the influence of field climate conditions and agricultural operations, it is difficult to maintain a level sufficient to control aphid damage, and its utilization methods are restricted. In recent years, my country's greenhouse vegetables and facility horticulture crops have developed rapidly. The closed environment and suitable temperature and humidity of greenhouses and greenhouses provide excellent conditions for using the seven-spotted ladybug to control aphids that occur on these crops, making the seven-starred ladybug widely used. prospect.

At present, Coccinella septempunctata are mainly raised using live aphids as feed, and some use artificial feed. Feeding methods using aphids require breeding aphids in large numbers, which is economically costly and wastes time. When artificial feed is fed, it will have a certain impact on the body weight, pupation rate, eclosion rate, etc. of the ladybug. In particular, low fecundity has become a bottleneck restricting the development of artificial feed for the ladybird.

Contents of the invention

In order to solve the above technical problems, the purpose of the present invention is to provide a composition for feeding Coccinella septempuncata and its preparation method and feed. The main component of the composition is an aphid treatment material, which has relatively comprehensive basic nutrition and ensures that Coccinella septempuncata is fed. of health.

On the one hand, the present invention provides a composition for feeding Coccinella septempuncata, which composition includes the following components by mass: 500-1000 parts of aphid treatment, 1-4 parts of β-carotene and 0.000001 parts of juvenile hormone ~0.00001 copies;

Among them, the aphid treatment product is obtained by crushing adult aphids into a slurry and mixing it with purified water in a ratio of 1:3.

Preferably, the composition includes the following components by mass: 700 to 800 parts of aphid treatment, 1.5 to 2.5 parts of beta carotene, and 0.000003 to 0.00006 parts of juvenile hormone.

Further, the composition includes the following components by mass: 500 to 1000 parts of aphid treatment, 1 to 4 parts of beta carotene, 0.000001 to 0.00001 parts of juvenile hormone, 50 to 300 parts of sucrose, and 1 to 100 parts of potassium sorbate. 5 servings;

Preferably, the composition includes the following components by mass: 700-800 parts of aphid treatment, 1.5-2.5 parts of β-carotene, 0.000003-0.00006 parts of juvenile hormone, 100-200 parts of sucrose and 1.5-200 parts of potassium sorbate. 2.5 servings.

Further, the adult aphids are obtained by inoculating aphids with reproductive capacity onto the host plant and collecting them when the aphid coverage rate of the host plant exceeds 70%.

On the other hand, the present invention provides a method for preparing the composition according to any one of the above, which method includes the following steps:

(1) Mix the aphid treatment material with sucrose, potassium sorbate, and purified water to obtain a first solution;

(2) Mix beta carotene with purified water to obtain a second solution;

(3) Mix the first solution, the second solution and juvenile hormone to obtain the composition.

On the other hand, the present invention provides a feed for feeding Coccinella septempuncata, which feed includes the composition described in any one of the above or the composition obtained by the above preparation method.

On the other hand, the present invention provides a method of feeding Coccinella septempunctata, using the composition according to any one of the above claims or the composition obtained by the above-mentioned preparation method or the above-mentioned feed.

Further, the container used is selected from a petri dish, a plastic box or a beverage cup; the container is provided with a lid or a slit with a through hole, and the through hole or slit is required to allow ventilation but not allow Coccinella septempuncata to pass through.

Further, the usage amount of the composition is 0.2g/bird, which is the recommended feed amount for raising a pair of male and female Coccinella septempunctata. This usage amount can ensure that the feed is available for the Coccinella septempunctata within one day. The state of eating. Using too much will not only cause waste, but also increase the humidity in the container and affect the sanitary conditions in the container. If the amount is too small, the feed will dry out quickly and reduce the normal feeding period.

Beneficial effects of the present invention:

(1) The components of the feed composition involved in the present invention include an aphid treatment, juvenile hormone and β-carotene. The aphid treatment has comprehensive basic nutrition, the juvenile hormone promotes the development of adult ovaries, and β-carotene improves the performance of insects. Survival rate and the ability to protect follicles and uterine cells. There is no significant difference in the number of eggs laid and hatching rates between ladybugs fed using this composition and those fed with conventional bean aphids, and the number of aphids required is relatively reduced. ; Its preparation method is simple, easy to scale and mechanize operation, the finished product is easy to store, and can be promoted on a large scale.

(2) The feed related to the present invention, after using the feed to raise Coccinella septempunctata, its feeding frequency is higher, and the demand for aphids is reduced by about 20% without significant reduction in egg laying and hatching rate, which saves space. More than 50%, artificial reduction of more than 30% has greatly improved the breeding efficiency of adult ladybugs.

Description of the drawings

Figure 1 shows the effects of different contents of β-carotene of the present invention on the feeding frequency of Coccinella septempuncata;

Figure 2 shows the effects of different contents of β-carotene of the present invention on the number of eggs laid by Coccinella septempuncata;

Figure 3 shows the effects of different contents of β-carotene of the present invention on the hatching rate of Coccinella septempuncata eggs;

Figure 4 shows the effects of different contents of juvenile hormone of the present invention on the feeding frequency of Coccinella septempuncata;

Figure 5 shows the effects of different contents of juvenile hormone of the present invention on the egg production of Coccinella septempuncata;

Figure 6 shows the effects of different contents of juvenile hormone of the present invention on the hatching rate of Coccinella septempunctata eggs;

Figure 7 shows the effects of different contents of aphid treatments of the present invention on the feeding frequency of Coccinella septempuncata;

Figure 8 shows the effects of different contents of the aphid treatment materials of the present invention on the number of eggs laid by Coccinella septempuncata;

Figure 9 shows the effects of different contents of aphid treatment materials of the present invention on the hatching rate of Coccinella septempunctata eggs.

Detailed ways

The present invention will be further described below by means of examples and drawings, but the present invention is not limited to the scope of the examples. Experimental methods that do not indicate specific conditions in the following examples should be selected according to conventional methods and conditions, or according to product specifications.

In the following examples, unless otherwise stated, all reagents and materials used are conventionally commercially available.

A kind of feed for feeding Coccinella septempuncata, the feed includes the following components by mass: 500-1000 parts of aphid treatment, 1-4 parts of β-carotene, 0.000001-0.00001 parts of juvenile hormone, 50-300 parts of sucrose and 1 to 5 parts of potassium sorbate; preferably, the composition includes the following components by mass: 700 to 800 parts of aphid treatment, 1.5 to 2.5 parts of beta carotene, 0.000003 to 0.000006 parts of juvenile hormone, and 100 parts of sucrose. ~200 parts and 1.5~2.5 parts of potassium sorbate.

Among them, the aphid treatment product is obtained by crushing adult aphids into a slurry and mixing it with pure water at a ratio of 1:3. The adult aphids are obtained by inoculating aphids with reproductive capacity onto the host plant. When the aphid coverage of the host plant is Collect it when it exceeds 70%. Adult aphids are larger in size and have weaker clinging power than nymphs, so they are easy to shake off. Shake off the grown adult aphids into a gauze container, and place the gauze net in a mortar to crush the adult aphids into powder. .

The preparation method of the above composition includes the following steps:

(1) Mix the aphid treatment material with sucrose, potassium sorbate, and purified water to obtain a first solution;

(2) Mix beta carotene with purified water to obtain a second solution;

(3) Mix the first solution, the second solution and juvenile hormone, add an appropriate amount of purified water to adjust the volume, and obtain the composition. (Juvenile hormone is a low-concentration and high-efficiency hormone substance and is insoluble in water. It needs to be The organic solvent (75% alcohol) is dissolved into a higher concentration solution and then added. Since the overall content of juvenile hormone is very low, the solvent used will not affect the breeding of Coccinella septempunctata). The mixture is initially made into a paste. The resulting mixture is filled into a semi-ellipsoid container with a diameter of 0.5-2.0cm and a height of 0.2-0.5cm. It is sealed with a sealing film and placed under low temperature conditions of 5-10°C. Storage, storage time is 5-10 days. After storage, the mixture becomes solid, which is convenient for feed replacement. After feeding, the mixture will gradually melt and be eaten by the seven-spotted ladybug.

When feeding Coccinella septempunctata, the container used can be a petri dish, a plastic box or a drink cup. The volume of the container is 10-2000ml. A gauze or material with gauze can be used instead of the lid or box so that the air in the container can be Normal exchange can prevent the adult ladybugs in the container from dying of hypoxia. This experiment mainly used a petri dish with a diameter of 9cm and a height of 2cm.

Add a new piece of artificial feed (0.2g) for each feeding, and process the remaining artificial feed to prevent deterioration from affecting the normal growth and reproduction of the seven-spotted ladybug. A small amount of fresh aphids (50 grams per feeding) needs to be added every 5-10 days. -100 aphids), supplement the nutrients missing from the ground aphids and ensure the health of the seven-spotted ladybug.

Example 1

In order to clarify the optimal concentration of β-carotene, the following experiments were designed:

Taking the basic formula (750g of aphid treatment + 150g of sucrose + 100ml of purified water + 2g of potassium sorbate) as the main body, a total of 5 treatment groups were designed: A, B, C, D and E. A is the basic formula plus 0.5g of β-carotene, B For the basic formula, add 1.0g of β-carotene, for C, add 1.5g of β-carotene, for D, add 2.0g of β-carotene, and for E, add 2.5g of β-carotene. Controlled trials of pure feed and pure aphid feeding were conducted simultaneously. The test lasted for 30 days. The feeding status, egg laying volume and hatching status of each group of ladybugs were observed to screen out the optimal β-carotene concentration.

The indoor environment is set to a temperature of 25 ± 1°C, a humidity of 75%, and 16 h/d of light. Use a petri dish as a breeding container and place filter paper on the bottom of the petri dish to prevent excessive humidity in the dish and also facilitate egg collection. Raise seven-spotted ladybugs in the order of 5 days of feed and 1 day of aphids (no feed is required when feeding aphids), and change the feed once a day. The specific treatment is as follows:

Treatment A: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 0.5g of β-carotene. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment B: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 1.0g of β-carotene. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment C: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 1.5g of β-carotene. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment D: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 2.0g of β-carotene. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment E: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 2.5g of β-carotene. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Pure feed control group: 750g of aphid treatment, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 1.5g of β-carotene, 0.2g of feed per day, and no fresh aphids;

Pure aphid control group: 200-300 aphids were fed every day and no feed was fed.

The results of the effect of using different contents of β-carotene on the feeding frequency of Coccinella septempunctata are shown in Figure 1. From the results in Figure 1, it can be seen that during the entire observation period, the average feeding frequency of Coccinella septempunctata in the five treatment groups and the pure feed group was The feeding frequency from high to low is: D>B>A>E>pure feed>C, in order 0.383, 0.325, 0.316, 0.249, 0.192, 0.166 times/min.

The results of the effect of using different contents of β-carotene on the egg production of Coccinella septempunctata are shown in Figure 2. From the results in Figure 2, it can be seen that during the entire observation period, the 5 treatment groups, the CK group and the pure feed group were fed Coccinella septempunctata. The number of eggs laid by insects from high to low is: CK>D>E>C>B>A>pure feed, followed by 51.2, 46.3, 41.6, 39.0, 23.3, 22.2, 5.2 eggs. The number of eggs laid by Coccinella septempuncata in groups A and B was extremely significantly lower than that in group CK (p<0.01). The number of eggs laid by Coccinella septempunctata in groups C, D and There was no significant difference in the number of eggs laid by insects (P>0.05).

The results of the effect of using different contents of β-carotene on the hatching rate of Coccinella septempunctata eggs are shown in Figure 3. From the results in Figure 2, it can be seen that during the entire recording period, the 5 treatment groups, the CK group and the pure feed group were fed with Coccinella septempunctata. The average hatchability of ladybugs from high to low is: E>B>D>A>CK>C>pure feed, followed by 86.0%, 82.1%, 79.6%, 77.1%, 76.5%, 75.3%, 2.2%. There was no significant difference in hatchability of egg masses between artificial diets A, B, C, D and E (P>0.05).

Example 2

In order to clarify the optimal concentration of juvenile hormone, the following experiments were designed:

Studies have shown that low concentrations of juvenile hormone have the property of promoting the development of insect ovaries, but high concentrations of juvenile hormone will inhibit the normal growth and development of insects. If the addition concentration of juvenile hormone that has a beneficial effect on ladybirds is clarified, it can also It can improve the egg-laying efficiency of seven-star ladybugs.

The test is based on the basic formula (750g of aphid treatment + 150g of sucrose + 100ml of purified water + 2g of potassium sorbate). A total of 5 treatment groups are designed: A, B, C, D, and E. A is the basic formula plus 1 μL of juvenile hormone, and B Add 5 μL of juvenile hormone to the basic formula, add 10 μL of juvenile hormone to the basic formula of C, add 20 μL of juvenile hormone to the basic formula of D, and add 50 μL of juvenile hormone to the basic formula of E. Control experiments of pure feed and pure aphid feeding are conducted at the same time. . The test lasted for 30 days. The feeding status, egg laying volume and hatching status of each group of ladybugs were observed to screen out the optimal juvenile hormone concentration.

The indoor environment is set to a temperature of 25 ± 1°C, a humidity of 75%, and 16 h/d of light. Use a petri dish as a breeding container and place filter paper on the bottom of the petri dish to prevent excessive humidity in the dish and also facilitate egg collection. Feed ladybugs in the order of 5 days of feed and 1 day of aphids (no need to add feed when feeding aphids), change the feed once a day, the specific treatment is as follows:

Treatment A: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 1μL of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment B: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 5μL of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment C: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 10μL of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment D: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 20μL of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Treatment E: 750g of aphid treatment material, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 50μL of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day;

Pure feed control group: 750g of aphid treatment, 150g of sucrose, 100ml of purified water, 2g of potassium sorbate, 10μL of juvenile hormone, 0.2g of feed per day, and no fresh aphids;

Pure aphid control group: 200-300 aphids were fed every day and no feed was fed.

The results of using different juvenile hormone contents on the feeding frequency of Coccinella septempunctata are shown in Figure 4. From the results in Figure 4, it can be seen that during the entire observation period, the average feeding frequency of Coccinella septempunctata in the five treatment groups and the pure feed group was The food frequency from high to low is: B>A>C>D>Pure feed>E, that is, 0.293, 0.276, 0.232, 0.211, 0.177, 0.175 times/min.

The results of using different juvenile hormone contents on the egg production of Coccinella septempunctata are shown in Figure 5. From the results in Figure 5, it can be seen that during the entire observation period, the 5 treatment groups, the CK group and the pure feed group were fed to Coccinella septempunctata. The number of eggs laid from high to low is: CK>B>A>C>E>D>pure feed, followed by 43.9, 36.6, 34.8, 31.5, 28.0, 26.9, and 2.5 eggs. The number of eggs laid by Coccinella septempuncata in groups C, D and E was significantly lower than that in group CK (0.01＜p<0.05). There was no significant difference in the number of eggs laid by ladybugs (P>0.05).

The results of using different juvenile hormone contents on the hatching rate of Coccinella septempunctata eggs are shown in Figure 6. From the results in Figure 6, it can be seen that during the entire recording period, the 5 treatment groups, the CK group and the pure feed group were fed Coccinella septempunctata. The average hatching rate of insects from high to low is: CK>B>A>C>D>E>pure feed, followed by 71.0%, 58.1%, 56.4%, 49.3%, 23.0%, 16.9%, 6.4%. The egg-laying rate of Coccinella septempunctata in groups D and E was extremely significantly lower than that of the CK group (P<0.01). The egg-laying rate of Coccinella septempunctata in groups A, B and C was significantly lower than that of the CK group. Hatching rate of Coccinella septempunctata (0.01＜p＜0.05).

To sum up, in this trial, the β-carotene artificial feed groups C, D, and E had better effects. The optimal content of β-carotene was 1.5-2.5g/L, and the optimal content was group D. is 2g/L. Juvenile hormone group Artificial feed groups A, B, and C have better effects. The optimal content of juvenile hormone is 1-10 μL/L. Group B is the best, and the optimal content is 5 μL/L.

Example 3

In order to clarify the feeding effect of the overall formula, the following experiments were designed:

On the premise of determining that the reasonable content of β-carotene is 1.5-2.5g/L and the content of juvenile hormone is 5 μL/L (because the content of juvenile hormone is too low, the optimal content in Experiment 2 is 5 μL/L) Next, design the treatment groups: C1, C2, C3, C4, C5, D1, D2, D3, D4, D5, E1, E2, E3, E4 and E5; groups C, D and E respectively correspond to those in Experiment 1. 1.5g/L, 2.0g/L and 2.5g/L carotene, groups 1-5 added aphid treatment and sucrose at mass ratios of 1:2, 1:1, 2:1, 4:1, 8 respectively. :1 aphid sucrose mixture. The indoor environment is set to a temperature of 25 ± 1°C, a humidity of 75%, and 16 h/d of light. Use a petri dish as a breeding container and place filter paper on the bottom of the petri dish to prevent excessive humidity in the dish and also facilitate egg collection. Feed the ladybugs in the order of 5 days of feed and 1 day of aphids (no feed is needed when feeding aphids), and change the feed once a day. The test lasted for 30 days, and the groups with higher feeding frequency, egg laying volume and hatching rate were not significantly different from the aphid control group to determine the final formula ratio.

The specific processing is as follows:

Treatment C1: 300g of aphid treatment material, 600g of sucrose, 100ml of purified water, 1.5g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment C2: 450g of aphid treatment material, 450g of sucrose, 100ml of purified water, 1.5g/L of β-carotene, 5μL/L of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day. ;

Treatment C3: 600g of aphid treatment material, 300g of sucrose, 100ml of purified water, 1.5g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment C4: 720g of aphid treatment material, 180g of sucrose, 100ml of purified water, 1.5g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment C5: 800g of aphid treatment material, 100g of sucrose, 100ml of purified water, 1.5g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment D1: 300g of aphid treatment material, 600g of sucrose, 100ml of purified water, 2.0g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment D2: 450g of aphid treatment material, 450g of sucrose, 100ml of purified water, 2.0g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment D3: 600g of aphid treatment material, 300g of sucrose, 100ml of purified water, 2.0g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment D4: 720g of aphid treatment material, 180g of sucrose, 100ml of purified water, 2.0g/L of β-carotene, 5μL/L of juvenile hormone. Coccinella septempunctata were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment D5: 800g of aphid treatment, 100g of sucrose, 100ml of purified water, 2.0gg/L of β-carotene, 5μL/L of juvenile hormone. Coccinella septempunctata were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment E1: 300g of aphid treatment material, 600g of sucrose, 100ml of purified water, 2.5g/L of β-carotene, 5μL/L of juvenile hormone. Coccinella septempunctata were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment E2: 450g of aphid treatment material, 450g of sucrose, 100ml of purified water, 2.5g/L of β-carotene, 5μL/L of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day. ;

Treatment E3: 600g of aphid treatment material, 300g of sucrose, 100ml of purified water, 2.5g/L of β-carotene, 5μL/L of juvenile hormone. The ladybugs were raised in the order of 5 days of feed and 1 day of aphids, and 0.2g of feed was fed every day. ;

Treatment E4: 720g of aphid treatment material, 180g of sucrose, 100ml of purified water, 2.5g/L of β-carotene, 5μL/L of juvenile hormone. Feed the ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day. ;

Treatment E5: 800g of aphid treatment material, 100g of sucrose, 100ml of purified water, 2.5g/L of β-carotene, 5μL/L of juvenile hormone. Feed ladybugs in the order of 5 days of feed and 1 day of aphids, and feed 0.2g of feed every day. ;

Pure aphid control group: 200-300 aphids were fed every day and no feed was fed.

The results of the effect of using different contents of aphid treatments on the hatching rate of Coccinella septempunctata eggs are shown in Figure 7. From the results in Figure 7, it can be seen that during the entire recording period, the number of Coccinella septempunctata fed in the 15 treatment groups and the CK group was The ones with higher feeding frequency are: C4, C5, D4, D5, E3, E4, and E5. It can be seen that the ratio of aphids to sucrose is 4:1 to 8:1, which is more reasonable.

The results of the effect of using different contents of aphid treatments on the hatching rate of Coccinella septempunctata eggs are shown in Figure 8. From the results in Figure 8, it can be seen that during the entire recording period, the number of Coccinella septempunctata fed in the 15 treatment groups and the CK group was The ones with higher egg laying volume were: C5, D4, D5, and E5, and there was no significant difference with the control group. The egg laying volume of the other treatment groups was significantly lower than that of the control group. It can be seen that the ratio of aphids to sucrose is 8:1 which is more reasonable.

The results of the effect of using different contents of aphid treatments on the hatching rate of Coccinella septempunctata eggs are shown in Figure 9. From the results in Figure 9, it can be seen that during the entire recording period, the Coccinella septempunctata fed in the 15 treatment groups and the CK group There was not much difference in the hatching rate, which was basically maintained at the level of 65%-80%, and there was no significant difference from the control group.

The optimal ratio of aphid treatment group and sucrose in the feed formula of this application is 8:1. However, considering the cost and difficulty of raw material processing, the ratio of 4:1 can also be used for production. The feeding effect and cost are both low, so this Apply aphids to sucrose ratio of 4:1 to 8:1.

Therefore, the preferred feed formula in this application is: 700-800g of aphids, 100-200g of sucrose, 100mL of purified water, 2g of potassium sorbate, 2 (±0.5)g of β-carotene, and 5 μL of juvenile hormone. After using this formula to raise Coccinella septempunctata, its feeding frequency is higher, and the demand for aphids is reduced by about 20% without significant reduction in egg laying and hatching rate (according to feed production standards, 0.2g of feed Contains 0.0375g aphids, equivalent to about 175 aphids/day, which is lower than the 200-300 aphids/day required for conventional feeding), and saves more than 50% of space (the feed volume is small, compared with the currently used utilization zone) For feeding aphid plants, smaller containers can be used), and manual labor can be reduced by more than 30% (the feed can be fed directly after the feed is made, without the need to cut fresh aphid plants every time; and the feeding amount is small, and the cleaning is also Feeding with aphid plants is convenient and quick), which greatly improves the breeding efficiency of adult ladybugs.

The composition for feeding Coccinella septempunctata and its preparation method and feed provided by the present invention have been introduced in detail above. This article uses specific examples to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method and the core idea of the present invention. It should be noted that those skilled in the art can make several improvements and modifications to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the scope of the claims of the present invention.

Claims (3)

1. A composition for feeding coccinella septempunctata, which is characterized by comprising the following components in parts by mass: 700-800 g of aphid treatment substance, 100-200 g of sucrose, 100mL of purified water, 2g of potassium sorbate, 1.5-2.5g of beta-carotene and 5 mu L of juvenile hormone;

wherein, the aphid treatment substance is obtained by rolling adult aphids into slurry and mixing the slurry with purified water according to the proportion of 1:3; the adult aphids are obtained by inoculating reproductive aphids to a host plant and collecting the plant when the coverage of the plant aphids exceeds 70%.

2. A process for preparing a composition as claimed in claim 1, comprising the steps of:

(1) Mixing the aphid treated matter with sucrose, potassium sorbate and purified water to obtain a first solution;

(2) Mixing beta carotene with purified water to obtain a second solution;

(3) Mixing the first solution, the second solution and the juvenile hormone to obtain the composition.

3. A feed for feeding ladybug, characterized in that the feed comprises the composition of claim 1 or the composition obtained by the preparation method of claim 2.