CN113332357A

CN113332357A - Composition and preparation for improving cervical HPV infection symptoms and preparation method

Info

Publication number: CN113332357A
Application number: CN202110623920.9A
Authority: CN
Inventors: 冯驯
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-06-04
Filing date: 2021-06-04
Publication date: 2021-09-03
Also published as: CN114903944B; CN114903944A

Abstract

The invention discloses a composition and a preparation for improving cervical HPV infection symptoms and a preparation method thereof. The composition for improving cervical HPV infection symptoms comprises 50-70 parts of bupleurum virus and 5-20 parts of ginseng and peony anti-allergic agent; the bupleurum virus clear comprises: extracts of bupleuri radix, herba Andrographitis, rhizoma Polygoni Cuspidati, Scutellariae radix, fructus forsythiae and pericarpium Granati; the ginseng and peony anti-allergy agent comprises: radix Paeoniae extract, hydrolyzed ginsenosides and radix scrophulariae extract. The composition and the preparation are combined by two Chinese herbal medicine compounds, can resist virus, resist allergy and diminish inflammation, can promote blood circulation to remove blood stasis, improve body functions, condition and improve the microenvironment of vagina, and solves the problem of narrow treatment range of a single formula. The composition and the preparation can effectively relieve the cervical HPV infection symptoms of women, improve the cervical environment of women and improve the immunity, are directly acted on affected parts when being externally used, have high safety and quick response, and clinical tests show that the composition has good effect on preventing and improving the cervical HPV infection symptoms.

Description

Composition and preparation for improving cervical HPV infection symptoms and preparation method

Technical Field

The invention relates to the technical field of pharmaceutical preparations, in particular to a composition for improving cervical HPV infection symptoms, a preparation and a preparation method thereof.

Background

Cervical cancer is one of the most common malignant tumors threatening the health of women worldwide, about 52.8 ten thousand new cervical cancer cases occur each year worldwide, and the number of deaths related to cervical cancer reaches 26.6 ten thousand. About 13 ten thousand new cases of cervical cancer account for about 1/4 of the total number of new cases in the world every year in China, and although the screening, the prevention and the treatment of the cervical cancer are actively promoted in China in recent years, the morbidity and the mortality of the cervical cancer are always high, the morbidity age tends to be younger, and the economic burden brought to the society and families is still very serious.

Human Papillomavirus (HPV) is a non-enveloped icosahedral capsid protein virus, a spherical DNA virus, belonging to the genus papillomavirus. HPV infection has two characteristics: squamous epithelium and immune escape. According to the relationship between HPV infection and the risk of cervical cancer, the HPV infection is divided into a low-risk type and a high-risk type. The low-risk HPV mainly comprises HPV6, HPV11, HPV30, HPV42, HPV43 and the like, and the low-risk HPV mainly causes benign lesions such as cervical low-grade intraepithelial neoplasia and genital warts. The high-risk HPV mainly comprises HPV16, HPV18, HPV31, HPV33, HPV52 and the like, and the high-risk HPV mainly causes lesions such as cervical intraepithelial neoplasia, cervical cancer, external genital cancer and the like. HPV is widely present in nature, is susceptible to survive in moist and warm environments, and human being is its only host.

Any female with sexual activity may be infected and patients with cervical HPV infection are often asymptomatic. The infection rate of young women with active sexual life is highest, the infection probability of young women with active sexual life under the age of 30 years is 4-15%, most infections are in an HPV carrying state, carriers can be cleared through self immunity, the clearing time is about 8 months on average, but 10-15% of patients still have continuous virus development due to insufficient self defense ability, and cervical lesions are caused.

HPV preventive vaccines have been popularized and used in a plurality of countries, and good effects are obtained, but bivalent HPV vaccines are just approved in China. In view of the current situation of cervical cancer prevalence in China, work needs to be performed from two aspects of prevention and clinical diagnosis and treatment, so as to reduce the incidence of cervical diseases.

The current clinical diagnosis and treatment method aiming at HPV comprises the following steps: western medicine treatment, Chinese medicine treatment and combination of Chinese and Western medicine. Western medical treatments include physical therapy (laser, freezing, microwave, and ozone fumigation, etc.), surgical therapy (cervical ring electrotomy), which is prone to organic damage, and drug therapy (interferon, vaccine), which requires frequent vaccination.

The traditional Chinese medicine treatment mainly emphasizes the concept of dialectical treatment and treatment of both symptoms and root causes. The traditional Chinese medicine for treating HPV infection mostly plays a role by reducing the virus load, the traditional Chinese medicine oral medicine improves the overall immune function and the antiviral ability, and the traditional Chinese medicine external medicine mainly helps to eliminate cervical local viruses by improving the local immune environment of the cervix so as to achieve the optimal treatment effect.

The traditional Chinese and western medicine combined treatment mainly aims at eliminating viruses and destroying virus host cells in western medicine, and mainly aims at improving the immunity of an organism or a local microenvironment and resisting viruses in traditional Chinese medicine.

Cervical HPV infected patients mostly have no obvious symptoms, are manifested by much leucorrhea, yellow or white color, sticky or thin texture, or peculiar smell, or pruritus vulvae, or mold, trichomonas or nonspecific bacterial infection, and belong to the category of leukorrhagia in traditional Chinese medicine. The "leukorrhagia" name is proposed for the first time in the treatise on the disease source, and the leucorrhea causes the blood and the filthy liquid to be brought down due to the impairment of the blood and qi, the damage of the thoroughfare vessel and the conception vessel caused by overstrain. Renzhai Zhi Fang Lun records that "bleeding in the lower part is not stopped, which is called metrorrhagia, frequent flow of dirty fluid, which is called leukorrhagia". For the pathogenesis of leukorrhagia, the 'Fu-Bai-Wei is all damp syndrome recorded in Fu-Qing-Master Ladies' and named because the pulse is not restricted, it is named. Dampness is a yin pathogen, which tends to block qi movement and damage yang qi.

Therefore, impairment of spleen yang, dysfunction of spleen in transportation and transformation, and dampness obstructing the lower energizer are the basic pathogenesis of the disease. The Su Wen & Ci Fang Lun proposes that the healthy qi exists in the interior and the pathogenic factors cannot be dried, and the Su Wen & Yi Pi Lun says that the pathogenic factors are accompanied by deficiency of qi. Therefore, the cervical HPV is continuously infected, on one hand, due to the long-term invasion of damp turbidity evil toxin to the phylum; on the other hand, the body is deficient in healthy qi and unable to support the pathogens to go out, and they are located in the uterus. This indicates that persistent infection with HPV is essentially qi-deficient, marked by downward flow of damp-heat. Frequent maintenance of cervical care and cleaning is an important measure for preventing HPV infection, Chinese herbal medicines improve immunity of an organism and a local microenvironment so as to achieve the aim of resisting virus, and the traditional Chinese medicine is safe, effective, free from side effects, unique in advantages and gradually accepted by people.

At present, a great deal of attempt work is carried out on the traditional Chinese medicine in the aspect of cervical HPV infection resistance, including internal and external treatment, and a certain curative effect is achieved. However, the existing traditional Chinese medicine composition has poor effect of improving and relieving cervical HPV infection symptoms of women.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide the composition for improving the cervical HPV infection symptom, which can resist virus, resist allergy and diminish inflammation, promote blood circulation to remove blood stasis and regulate body functions, thereby achieving good effects of improving and relieving the cervical HPV infection symptom.

The invention also aims to provide a preparation for improving cervical HPV infection symptoms, which is used for improving and treating the HPV infection symptoms.

The invention also aims to provide a preparation method of the preparation for improving cervical HPV infection symptoms, which is simple in process and suitable for industrial production.

One of the purposes of the invention is realized by adopting the following technical scheme:

a composition for improving cervical HPV infection symptom comprises 50-70 parts of bupleurum virus and 5-20 parts of ginseng and peony anti-allergy agent; the bupleurum virus clear comprises the following components: extracts of bupleuri radix, herba Andrographitis, rhizoma Polygoni Cuspidati, Scutellariae radix, fructus forsythiae and pericarpium Granati; the ginseng and peony anti-allergy agent comprises the following components: radix Paeoniae extract, hydrolyzed ginsenosides and radix scrophulariae extract.

Further, the bupleurum virus clear also comprises the following components: butanediol and water; the ginseng and peony anti-allergy agent also comprises the following components: propylene glycol and water.

Further, the bupleurum virus clear comprises the following components: 15 parts of radix bupleuri, 15 parts of common andrographis herb, 15 parts of giant knotweed, 15 parts of baical skullcap root, 15 parts of weeping forsythia capsule, 15 parts of pomegranate bark extract, 150 parts of butanediol and 150 parts of water.

Further, the ginseng and peony anti-allergy agent comprises the following components: 2-3 parts of radix paeoniae alba extract, 2-3 parts of hydrolyzed ginsenosides, 2-3 parts of radix scrophulariae extract, 70-78 parts of propylene glycol and 15-20 parts of water.

Further, the lactobacillus fermented product also comprises 20-40 parts.

The second purpose of the invention is realized by adopting the following technical scheme:

a preparation for improving cervical HPV infection symptoms comprises 50-70 parts of bupleurum virus, 5-20 parts of radix paeoniae alba anti-allergy agent and 1000 parts of excipient 100-.

Further, the lactobacillus fermented product also comprises 20-40 parts.

Further, the excipient comprises 900 parts of water 700-.

Further, the preparation is a solution, an ointment, a gel, an aerosol, a tablet, a capsule or a powder.

The third purpose of the invention is realized by adopting the following technical scheme:

a preparation method of a preparation for improving cervical HPV infection symptoms comprises the following components in parts by weight: 50-70 parts of bupleurum virus, 5-20 parts of radix paeoniae alba anti-allergy agent, 20-40 parts of lactobacillus fermentation product, 900 parts of water 700-; the preparation method comprises the following steps:

a swelling step: adding water with the formula amount into a liquid preparation tank, and then adding carbomer with the formula amount to swell the carbomer;

an active ingredient addition step: adding the bupleurum virus clearing agent, the ginseng and peony anti-allergic agent and the lactobacillus fermentation product into the liquid preparation tank according to the formula amount, and stirring;

and (3) adding auxiliary materials: adding the propylene glycol with the formula amount, and uniformly stirring; and adding triethanolamine and ethyl glycerol according to the formula amount while stirring, and uniformly stirring to obtain a light yellow transparent gel matrix.

Compared with the prior art, the invention has the beneficial effects that:

(1) the composition for improving cervical HPV infection symptoms provided by the invention is a composite composition formed by combining two traditional Chinese medicine compounds, can resist viruses, resist allergy and diminish inflammation, can promote blood circulation to remove blood stasis, improve body functions, condition and improve vaginal microenvironment, and solves the problem of narrow treatment range of a single formula. The composition can effectively relieve the cervical HPV infection symptoms of women, improve the cervical environment of women and improve the immunity, is directly acted on an affected part when being externally used, has high safety and quick response, and clinical tests show that the composition has good effect on preventing and improving the cervical HPV infection symptoms.

(2) The preparation for improving cervical HPV infection symptoms provided by the invention adopts a traditional Chinese medicine compound, and has good effects on improving and treating female cervical HPV.

(3) The preparation method of the preparation for improving cervical HPV infection symptoms provided by the invention is simple in process and suitable for industrial production.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment. The raw materials, equipments and the like used in the following examples are commercially available unless otherwise specified. For example, the raw material medicines used in the embodiment of the invention can be purchased from traditional Chinese medicine stores and traditional Chinese medicine markets, and the specifications of the raw material medicines meet the national traditional Chinese medicine pharmacopoeia standards. And all the Chinese herbal medicine extracts are in the catalogue of 2021 edition of cosmetic raw materials.

A composition for improving cervical HPV infection symptom comprises 50-70 parts of bupleurum virus and 5-20 parts of ginseng and peony anti-allergy agent; the bupleurum virus medicament comprises the following components: extracts of bupleuri radix, herba Andrographitis, rhizoma Polygoni Cuspidati, Scutellariae radix, fructus forsythiae and pericarpium Granati; the ginseng and peony anti-allergy agent comprises the following components: radix Paeoniae extract, hydrolyzed ginsenosides and radix scrophulariae extract. The bupleurum virus clearing and ginseng and peony anti-allergic agent is a Chinese medicinal compound preparation which is developed according to the theoretical design formula of the Chinese medicine and combining the use effect of patients. The compound of the bupleurum virus clearing and ginseng and peony anti-allergic agent is specially used for patients with cervical HPV infection or patients with slight symptoms, has certain curative effect on female leukorrhagia, yellow or white color, sticky or thin texture, peculiar smell, pruritus vulvae, or infection accompanied by mould, trichomonas or non-specific bacteria, and obviously improves and relieves the symptoms of the female cervical HPV infection.

Description of the efficacy of bupleurum viruses: the Chinese medicinal components of the bupleurum root virus medicine include bupleurum root, creat, giant knotweed rhizome, skullcap root, forsythia fruit and pomegranate rind extract. According to the theory of traditional Chinese medicine, the traditional Chinese medicines with the effects of clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, and relieving swelling and promoting diuresis are utilized, and the main effects are as follows: antiviral and symptom relieving effects.

Bupleurum root: the property of bupleurum is bitter, pungent and slightly cold, and the bupleurum enters liver and gallbladder channels, has the effects of relieving exterior syndrome, reducing fever, soothing liver, relieving depression and lifting yang qi, and can be clinically used for treating cold and fever, alternating cold and heat, distending pain in chest and hypochondrium, irregular menstruation, dysmenorrheal, uterine prolapse, rectocele and the like. In addition, it can also be used for treating depression.

Andrographis paniculata: also called Chunlian autumn willow, once you please, elemena, scabrous elephantfoot herb, lysimachia foenum-graecum hance, golden fungus hook, indian grass, eel grass, etc. The medicinal plants have the functions of clearing away heat and toxic materials, diminishing inflammation, diminishing swelling and relieving pain, and particularly have the curative effects of treating various infectious diseases, including traumatic infection, furuncle, carbuncle, erysipelas, upper respiratory tract infection, acute and chronic tonsillitis, acute and chronic pharyngolaryngitis, acute and chronic bronchitis, acute bacillary dysentery, acute gastroenteritis, urinary tract infection, endometritis, pelvic inflammation, otitis media, periodontitis and the like, with different degrees. The preparation and the use method are as follows: it is now available in the form of injection, oral capsule or tablet, and external preparation.

Giant knotweed rhizome: the rhizome is used for medicine, and has the effects of promoting blood circulation, removing blood stasis, dredging channels, relieving cough, etc. The functional indications are as follows: dispel wind and remove dampness, dissipate blood stasis and relieve pain, relieve cough and resolve phlegm. Can be used for treating arthralgia, jaundice due to damp-heat pathogen, amenorrhea, abdominal mass, scald due to hot water or fire, traumatic injury, carbuncle, swelling, sore, and cough with excessive phlegm.

Scutellaria baicalensis: it is bitter and cold in taste, and has effects of clearing heat and eliminating dampness, purging pathogenic fire and removing toxic substance, stopping bleeding, preventing miscarriage, etc. It can be used for treating epidemic febrile disease, upper respiratory infection, cough due to lung heat, yellow gallbladder due to damp-heat, pneumonia, dysentery, hemoptysis, conjunctival congestion, threatened abortion, hypertension, carbuncle, furuncle, and sore. The clinical antibacterial property is better than that of coptis root, and the drug resistance is not generated.

Fructus forsythiae: the functions are mainly used for clearing heat, detoxifying, dissipating stagnation and reducing swelling. It is indicated for warm heat, erysipelas, macula, superficial infection, abscess, swelling, scrofula and stranguria. The book of the drug property treatise records that the recipe is mainly used for treating stranguria caused by five kinds of stranguria and urinary obstruction, and can remove heart heat and heat in the family.

Pomegranate rind: has the effects of relieving diarrhea with astringents, stopping bleeding, and expelling parasites. It is commonly used for chronic diarrhea, chronic dysentery, hematochezia, rectocele, metrorrhagia, leukorrhagia, abdominal pain due to parasitic infestation.

The traditional Chinese medicine formula for treating the bupleurum virus has the effects of soothing liver and relieving depression, improving yang, clearing away heat and toxic materials, diminishing inflammation and relieving pain, stopping bleeding and preventing miscarriage, and relieving diarrhea and stranguria, and can be used for improving cervical diseases caused by HPV infection.

Explanation of efficacy of Shenshao anti-allergic agent: according to the theory of traditional Chinese medicine, the ginseng and peony anti-allergy agent utilizes the treatment concept of nourishing and dredging collaterals, and has the main effects of improving immunity, dredging collaterals, activating blood and relieving symptoms.

Peony: root drug, called "Bai Shao", has the actions of alleviating pain, relieving spasm, removing blood stasis and restoring menstrual flow.

Ginseng: the root is tonic, and is suitable for regulating blood pressure, restoring cardiac function, neurasthenia and asthenia, and also has expectorant, stomach invigorating, diuretic and exciting effects.

Figwort root: heat enters nutrient and blood in warm diseases; fever; polydipsia; a deep-red tongue; carrying out spot formation; bone steaming, strain cough; restlessness due to deficiency in the middle energizer; constipation due to body fluid impairment; eyes are astringent and dim; swollen and sore throat; scrofula and subcutaneous nodule; abscess, deep-rooted carbuncle and sore.

The three Chinese medicinal materials are extracted to enrich effective components, and are redissolved with alcohol water.

Preferably, the bupleurum viral clear also comprises the following components: butanediol and water, wherein the butanediol and the water are used as a solvent and an extracting agent; the ginseng and peony anti-allergy agent also comprises the following components: propylene glycol and water, wherein the propylene glycol and the water are used as a solvent and an extracting agent.

Preferably, the bupleurum viral clear comprises the following components: 15 parts of radix bupleuri, 15 parts of common andrographis herb, 15 parts of giant knotweed, 15 parts of baical skullcap root, 15 parts of weeping forsythia capsule, 15 parts of pomegranate bark extract, 150 parts of butanediol and 150 parts of water. The dosage of each active component of the bupleurum virus medicament is within the limited range, and the good synergistic effect can be exerted, so that the cervical disease caused by HPV infection can be better improved and relieved.

Preferably, the ginseng and peony anti-allergy agent comprises the following components: 2-3 parts of radix paeoniae alba extract, 2-3 parts of hydrolyzed ginsenosides, 2-3 parts of radix scrophulariae extract, 70-78 parts of propylene glycol and 15-20 parts of water. The dosage of each active component of the ginseng and peony anti-allergy agent is within the limited range, so that the ginseng and peony anti-allergy agent can play a good synergistic effect, and can better improve and relieve cervical diseases caused by HPV infection.

Preferably, the feed also comprises 20-40 parts of lactobacillus fermentation products. The lactobacillus fermentation liquor can increase the growth of beneficial bacteria and regulate vaginal flora balance. The lactic acid bacteria fermentation product can be purchased on the market, and is preferably a lactic acid bacteria fermentation product of microorganism health biological science and technology limited company.

The applicant named chaisheishu as a composition for improving cervical HPV infection symptoms provided by the embodiment of the invention combines two traditional Chinese medicine compounds into a composite composition, which can resist virus, resist allergy and diminish inflammation, promote blood circulation to remove blood stasis, improve body functions, condition and improve vaginal microenvironment, and fill the problem of narrow treatment range of a single formula. The composition can effectively relieve the cervical HPV infection symptoms of women, improve the cervical environment of women and improve the immunity, is directly acted on an affected part when being externally used, has high safety and quick response, and clinical tests show that the composition has good effect on preventing and improving the cervical HPV infection symptoms.

A preparation for improving cervical HPV infection symptoms comprises 50-70 parts of bupleurum virus, 5-20 parts of radix paeoniae alba anti-allergy agent and 1000 parts of excipient 100-. The preparation takes the bupleurum virus clearing and the ginseng and peony anti-allergy agent as the effective components, and can be prepared into preparations of different dosage forms by adding corresponding auxiliary materials (excipients), so that the use requirements of different crowds can be met.

Preferably, the feed also comprises 20-40 parts of lactobacillus fermentation products. The lactobacillus fermentation liquor can increase the growth of beneficial bacteria, regulate the balance of vaginal flora and is beneficial to enhancing the improvement and relieving effects of cervical HPV infection symptoms.

Preferably, the excipient comprises 900 parts of water 700-. The auxiliary materials are gel matrix excipients, and the product prepared by adding the excipients is a gel preparation which is convenient for a user to smear.

Preferably, the preparation is a solution, ointment, gel, aerosol, tablet, capsule or powder. In actual use, the preparation is preferably in the form of gel, but can also be prepared into solution for scrubbing; or making into ointment for application; preparing into aerosol for spraying; or, made into tablet and capsule for stuffing therapy; or, the powder preparation can be prepared and can be mixed with a solvent before use.

When the preparation for improving cervical HPV infection symptoms is a gel, the preparation comprises the following components in parts by weight: 50-70 parts of bupleurum virus, 5-20 parts of radix paeoniae alba anti-allergy agent, 20-40 parts of lactobacillus fermentation product, 900 parts of water 700-; at this time, the preparation method may be performed according to the following steps:

an active ingredient addition step: adding the bupleurum virus clearing agent, the ginseng and peony anti-allergic agent and the lactobacillus fermentation product into a liquid preparation tank according to the formula amount, and stirring;

Examples 1 to 3

A preparation for improving cervical HPV infection symptoms is prepared from bupleurum virus, radix Paeoniae alba anti-allergic agent, lactobacillus fermentation product and excipient, wherein the bupleurum virus is prepared from the following components: 15 parts of radix bupleuri, 15 parts of common andrographis herb, 15 parts of giant knotweed, 15 parts of baical skullcap root, 15 parts of weeping forsythia capsule, 15 parts of pomegranate bark extract, 150 parts of butanediol and 150 parts of water; the ginseng and peony anti-allergy agent is prepared from the following components: 2.5 parts of a peony root extract, 2.5 parts of hydrolyzed ginsenoside, 2.5 parts of a figwort root extract, 75 parts of propylene glycol and 18 parts of water; the excipient is gel excipient, and the finally formed product is the gel preparation for external vagina application. The specific formulation table is detailed in table 1.

TABLE 1 examples 1-3 formulation of formulations for ameliorating symptoms of cervical HPV infection

Examples 1-3 the formulations were prepared as follows:

example 1

(1) 860 parts of water is added into a 3000L liquid preparation tank, and then 7 parts of carbomer with the formula amount is slowly added to swell the carbomer;

(2) adding 70 parts of bupleurum virus clear and 5 parts of ginseng and peony anti-allergy agent according to the formula amount, and uniformly stirring; then adding 30 parts of lactobacillus fermentation product according to the formula amount, and uniformly stirring;

(3) adding 20 parts of propylene glycol according to the formula amount, and uniformly stirring; then slowly adding 8 parts of triethanolamine and 5 parts of ethyl glycerol according to the formula amount while stirring, and uniformly stirring to obtain a light yellow transparent gel matrix;

(4) and (6) subpackaging.

Example 2

(2) adding 60 parts of bupleurum virus clearance and 10 parts of ginseng and peony anti-allergy agent according to the formula amount, and uniformly stirring; then adding 30 parts of lactobacillus fermentation product according to the formula amount, and uniformly stirring;

(4) and (6) subpackaging.

Example 3

(1) Adding 900 parts of water into a 3000L liquid preparation tank, and slowly adding 7 parts of carbomer according to the formula amount to swell the carbomer;

(2) adding 50 parts of bupleurum virus clear and 20 parts of ginseng and peony anti-allergy agent according to the formula amount, and uniformly stirring; then adding 30 parts of lactobacillus fermentation product according to the formula amount, and uniformly stirring;

(3) adding 10 parts of propylene glycol according to the formula amount, and uniformly stirring; then slowly adding 8 parts of triethanolamine and 5 parts of ethyl glycerol according to the formula amount while stirring, and uniformly stirring to obtain a light yellow transparent gel matrix;

(4) and (6) subpackaging.

Effect verification

First, physical shop tries

1. Subject design

In 2021, from 3 months to 5 months, the prescription and blank gel formula of the example 2 are respectively adopted to prepare samples, 5kg of the samples are prepared in total, the samples are subpackaged to form 1000 gel test packs, the gel test packs are respectively tested in two physical shops, and 50 females (37 in early infection stage and 13 in middle infection stage) are selected for test. Of these, 40 female patients (30 early stage infected patients and 10 middle stage infected patients) used the sample of example 2, and 10 female patients (7 early stage infected patients and 3 middle stage infected patients) used the sample of the control group. The blank gel formulation does not contain Chaishao component (does not contain bupleurum virus clearing, ginseng peony anti-allergic agent and lactobacillus fermentation product).

Table 2: subject design

Physical store	Early infected person	Middle stage infected person
			Physical store 1	12	6
Physical store 2	18	4
			Control group	7	3

2. Standard of therapeutic effect and effect of use

The curative effect standard is as follows: the treatment effect is mainly judged according to the following 3 indexes.

Whether the symptoms of erosion and pruritus are relieved or not;

whether viscous secretion is reduced;

and thirdly, whether the peculiar smell is improved or not.

According to the 3 indexes, the treatment effect is divided into 4 levels (healing, obvious effect, effective and ineffective), and the definition of the 4 levels is as follows:

and (3) healing: no erosion and pruritus symptoms, reduced secretion amount, normal amount recovery, no odor;

the effect is shown: no erosion and pruritus, reduced viscous secretion, no odor;

the method has the following advantages: the erosion and pruritus symptoms are relieved, the viscous secretion is reduced, and no peculiar smell exists;

and (4) invalidation: the symptoms of erosion and pruritus are not relieved, the reduction of viscous secretion is not caused, and the peculiar smell is not improved.

3. Application method

The using method comprises the following steps: mainly comprises two steps of cleaning and external application, wherein the cleaning and external application is carried out for 2 times a day, a period is 7 days, and the cleaning and external application are continuously carried out for 2 periods.

Cleaning: cleaning the affected part with warm water and bath lotion, and washing;

externally applying: apply 5g of the powder to the affected part of vagina in two times.

4. Statistics of results

The statistics of the usage effect of the prescription of example 2 are shown in table 3 below, and the statistics of the usage effect of the control group samples are shown in table 4 below.

Table 3 example 2 demographics on the effectiveness of sample use

Group of	Number of examples	Recovery method	Show effect	Is effective	Invalidation
						Early infected person	30	27	3	0	0
Middle stage infected person	10	0	8	2	0

TABLE 4 statistics of the number of persons with the effect of using the control group samples

Group of	Number of examples	Recovery method	Show effect	Is effective	Invalidation	Self-healing
							Early infected person	7	0	0	0	6	1
Middle stage infected person	3	0	0	0	3	0

As can be seen from the recorded results in table 3, the composition and gel preparation for improving cervical HPV infection symptoms provided by the embodiment of the present invention have good therapeutic effect on HPV, and the symptoms of users in early and middle stages are relieved, especially the recovery rate of patients in early stage is high. Of the 40 test subjects who tried the sample of example 2, 27 early-stage infected subjects were cured; the symptoms of 3 early infected persons are relieved, and 8 middle infected persons are obviously improved, namely 11 cases of the early infected persons have obvious effects; the itching of 2 cases of middle-stage infected persons is improved, and the effective cases are shown.

As can be seen from the recorded results in table 4, 9 of the 10 subjects who tried the blank control group sample did not improve the symptoms, and 1 of the early patients were self-healed to meet the control conditions.

In conclusion, the preparation for improving cervical HPV infection symptoms provided by the embodiment of the invention has good effects of improving and relieving early and middle HPV infection symptoms of women, is safe and reliable, is convenient for family care, and has great market potential.

Second, typical case

1. Case 1, old women, age 42, HPV early infected, with itching, profuse secretions and off-flavors. The prescription of example 1 is tried, the application is carried out 2 times a day, and after 7 days, the pruritus symptom is relieved, and the viscous secretion is reduced; after the continuous use for 5 days, the pruritus symptom basically disappears, the secretion amount returns to normal, and no peculiar smell exists.

2. Case 2, xiao lady, 50 years old, HPV middle-term infected person, with erosive, pruritic symptoms, more secretion and peculiar smell. The prescription of the embodiment 3 is tried, the medicine is used for 2 times a day, and after the medicine is used for 7 days, the symptoms of erosion and pruritus are relieved, and the viscous secretion is reduced; after the medicine is continuously used for 7 days, erosion disappears, and the medicine has no pruritus symptom, nearly normal secretion amount and no peculiar smell.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. A composition for improving cervical HPV infection symptoms is characterized by comprising 50-70 parts of bupleurum virus and 5-20 parts of ginseng and peony anti-allergy agent; the bupleurum virus clear comprises the following components: extracts of bupleuri radix, herba Andrographitis, rhizoma Polygoni Cuspidati, Scutellariae radix, fructus forsythiae and pericarpium Granati; the ginseng and peony anti-allergy agent comprises the following components: radix Paeoniae extract, hydrolyzed ginsenosides and radix scrophulariae extract.

2. The composition for ameliorating the symptoms of cervical HPV infection of claim 1, wherein the bupleurum viras serum further comprises the following components: butanediol and water; the ginseng and peony anti-allergy agent also comprises the following components: propylene glycol and water.

3. The composition for ameliorating the symptoms of cervical HPV infection of claim 2, wherein the bupleurum viras comprises the following components: 15 parts of radix bupleuri, 15 parts of common andrographis herb, 15 parts of giant knotweed, 15 parts of baical skullcap root, 15 parts of weeping forsythia capsule, 15 parts of pomegranate bark extract, 150 parts of butanediol and 150 parts of water.

4. The composition for improving cervical HPV infection symptoms of claim 2, wherein the ginseng and peony anti-allergic agent comprises the following components: 2-3 parts of radix paeoniae alba extract, 2-3 parts of hydrolyzed ginsenosides, 2-3 parts of radix scrophulariae extract, 70-78 parts of propylene glycol and 15-20 parts of water.

5. The composition for ameliorating the symptoms of cervical HPV infection according to claim 1, further comprising 20-40 parts of a lactic acid bacteria fermentation product.

6. A preparation for improving cervical HPV infection symptoms is characterized by comprising 50-70 parts of bupleurum virus, 5-20 parts of ginseng and peony anti-allergy agent and 100-1000 parts of excipient.

7. The preparation for ameliorating the symptoms of cervical HPV infection according to claim 6, further comprising 20-40 parts of a lactic acid bacteria fermentation product.

8. The preparation for improving symptoms of cervical HPV infection according to claim 6, wherein the excipient comprises water 700-900 parts, propylene glycol 10-30 parts, ethyl glycerol 2-10 parts, carbomer 3-15 parts, triethanolamine 3-15 parts.

9. The formulation for ameliorating the symptoms of cervical HPV infection according to claim 6, wherein the formulation is a solution, ointment, gel, aerosol, tablet, capsule or powder.

10. A preparation method of a preparation for improving cervical HPV infection symptoms is characterized in that the preparation comprises the following components in parts by weight: 50-70 parts of bupleurum virus, 5-20 parts of radix paeoniae alba anti-allergy agent, 20-40 parts of lactobacillus fermentation product, 900 parts of water 700-; the preparation method comprises the following steps: