CN113188867A - Medical science inspection is with phlegm sample preprocessing device - Google Patents

Medical science inspection is with phlegm sample preprocessing device Download PDF

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Publication number
CN113188867A
CN113188867A CN202110470887.0A CN202110470887A CN113188867A CN 113188867 A CN113188867 A CN 113188867A CN 202110470887 A CN202110470887 A CN 202110470887A CN 113188867 A CN113188867 A CN 113188867A
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sputum
cup
cavity
sampler
culture
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CN113188867B (en
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吴玉丹
陈璐璐
张甜甜
郭宇
褚旭
蔡林衡
时振国
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First Affiliated Hospital of Henan University of Science and Technology
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First Affiliated Hospital of Henan University of Science and Technology
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/02Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34Purifying; Cleaning
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088Concentrating samples by other techniques involving separation of suspended solids filtration

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  • General Engineering & Computer Science (AREA)
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Abstract

The utility model provides a medical science inspection is with phlegm sample preprocessing device, includes casing and a plurality of phlegm cup of getting place the seat, each gets that has the chamber that holds of putting the phlegm cup in the phlegm cup place the seat, to getting to pour into compound enzymolysis liquid in the phlegm cup in order to carry out the syringe of preliminary treatment and penetrate and get phlegm cup bottom and will be through the sampler that breeds the expanding propagation in the bacterial culture mechanism that the pre-installation has the culture solution is derived to the phlegm sample of preliminary treatment. The sputum sample can be processed, so that bacteria in the sputum sample can be propagated in the queuing inspection process and can be detected more easily, the detection time of the sputum sample is greatly shortened, the detection efficiency is improved, and more importantly, the micro pathogenic bacteria in the sputum sample can be detected more accurately; wherein the bottom of the sampler can be punctured when the sputum taking cup is put into the sputum taking cup, so that the diluted sputum after the enzymolysis reaction is dripped into the bacterial culture mechanism for culture after passing through a through channel in the sputum taking cup.

Description

Medical science inspection is with phlegm sample preprocessing device
Technical Field
The invention relates to the field of sputum sample inspection in the medical field, in particular to a sputum sample pretreatment device for medical inspection.
Background
Sputum is the secretion of alveoli, bronchi and trachea, and the amount of sputum is very small in healthy people. In pathological conditions, after respiratory mucosa and alveoli are stimulated, the mucous membranes are congested and edematous, serous fluid is exuded, mucus secretion is increased, and exudates such as various cells and fibrin are mixed with mucus, inhaled dust, certain tissue necrotic matters and the like to form sputum. The composition of sputum is complex and consists of 95% moisture and 5% dust, protein, etc.
The sputum examination has certain value for diagnosis, observation of curative effect and prognosis of respiratory system diseases such as pulmonary tuberculosis, lung fluke, lung tumor, bronchial asthma, chronic bronchitis, bronchiectasis and the like.
During sputum examination, a sputum sample of a patient is generally extracted and then sent to a clinical laboratory, and the types of bacteria in the sputum are analyzed by an instrument so as to judge the types of diseases; however, in hospitals, since there are too many patients to be examined, it takes a long time to queue up a sputum sample for examination after delivery, and therefore, it is generally necessary to temporarily store the sputum sample in a refrigerated state and then conduct examination after treatment, which results in a prolonged examination time.
Disclosure of Invention
In order to solve the problem that the inspection time is prolonged after the existing sputum sample is refrigerated and stored, the invention provides a sputum sample pretreatment device for medical inspection, which can treat the sputum sample, so that bacteria in the sputum sample can be propagated in the queuing inspection process and can be detected more easily, the detection time of the sputum sample is greatly shortened, the detection efficiency is improved, and more importantly, the micro pathogenic bacteria in the sputum sample can be detected more accurately.
The technical scheme adopted by the invention for solving the technical problems is as follows: a sputum sample pretreatment device for medical examination comprises a shell and a plurality of sputum taking cup placing seats arranged in the shell, wherein each sputum taking cup placing seat is internally provided with a containing cavity for placing a sputum taking cup, an injector for injecting compound enzymatic hydrolysate into the sputum taking cup to pretreat a sputum sample in the sputum taking cup, and a sampler which penetrates the bottom of the sputum taking cup and guides the pretreated sputum sample out to a bacteria culture mechanism pre-filled with culture solution to cultivate and propagate; the sampler includes that the top has the cylinder that penetrates the toper pointed end in getting the phlegm cup, sets up the at least two passages that run through that supply the phlegm sample after the preliminary treatment to pass along the axial of cylinder, all is provided with in each passage that runs through and carries out filterable cassette to the phlegm sample.
As an optimization scheme of the sputum sample pretreatment device for medical examination, the center of the bottom of the conical tip protrudes out of the top end of the cylinder through a connecting piece, the top end of the cylinder is provided with a groove surrounding the connecting piece, the edge of the groove and the side wall of the cylinder form a scraper surrounding the conical tip, and the scraper and the bottom of the conical tip form an opening.
As another optimized scheme of the sputum sample pretreatment device for medical examination, the sampler is provided with a sterilization cleaning unit for cleaning and sterilizing the sampler, the sterilization cleaning unit comprises a main cleaning pipeline communicated with a cleaning water pump, a branch cleaning pipeline connected with the main cleaning pipeline and used for conveying cleaning water into each sampler, and an electric heating wire arranged in each sampler and used for heating the sampler, wherein the cleaning water pump is arranged in an equipment cavity at the lower part of the shell; the electric heating wire is arranged in a closed cavity which is arranged in the center of the column body and extends along the axial direction of the column body, the through channel is arranged around the closed cavity, a cleaning hole with the tail end communicated with the bottom end of the through channel is arranged in the center of the bottom of the column body, and the head end of the cleaning hole is communicated with the branch cleaning pipeline, so that cleaning water enters the through channel at a certain pressure to clean the through channel; the utility model discloses a pass through passageway, including the cassette, the cassette is woven for the wire and is formed the thin slice that has the gap, and the slip sets up in passing through the passageway, and the upper surface of cassette passes through the extension spring to be connected the annular wedge bottom that the inner wall was equipped with in the passageway top of passing through.
As another optimized scheme of the sputum sample pretreatment device for medical examination, the sputum taking cup is provided with a conical cup body, the top of the cup body is provided with a sealing cover, the bottom of the cup body is provided with a flow guide pipe matched with the sampler, the bottom end opening of the flow guide pipe is sealed by a thin film, and when the sputum taking cup is placed in the sputum taking cup placing seat, the top end of the sampler punctures the thin film; the side wall of the upper part of the cup body is provided with a disposable injection hole for the injector to puncture and penetrate into.
As another optimized scheme of the sputum sample pretreatment device for medical examination, the inside of the shell is divided into a sputum taking cup placing cavity, a culture dish taking and placing cavity, a culture solution storage cavity and a spare part box from top to bottom, wherein the sputum taking cup placing seat is arranged in the sputum taking cup placing cavity, an opening which can be opened and is sealed by a cover plate is formed in the top of the sputum taking cup placing cavity, and the sputum taking cup is placed in or taken out through the opening; the bacterial culture mechanism is arranged in the culture dish taking and placing cavity, and the side wall of the culture dish taking and placing cavity is provided with an openable window so as to take out or place the culture dish pre-filled with culture solution in the bacterial culture mechanism through the window; each culture dish taking and placing cavity is positioned below one sampler; the culture solution storage cavity is internally stored with culture solution, and the culture solution is conveyed into each culture dish through a pumping mechanism; the spare part case is an openable cavity, and a spare culture dish and a spare sputum taking cup are stored in the spare part case.
As above-mentioned medical science inspection is with another kind of optimization scheme of phlegm sample preprocessing device, get a plurality of rows of window direction regular distribution that the chamber was put along the culture dish to phlegm cup arrangement seat in the casing, each row gets the culture dish of phlegm cup arrangement seat below and connects as an organic wholely through the connecting piece to extend the window through handle portion, be convenient for pull out or push the culture dish through handle portion, the both sides of culture dish have along the track that the culture dish was taken out or was pushed the direction, below at the culture dish is provided with the heat preservation chamber, a temperature for keeping in the culture dish.
As another kind of optimization scheme of above-mentioned sputum sample preprocessing device for medical examination, the top that the chamber was got to the culture dish has a plurality of strip cloth sap cavity, and each strip cloth sap cavity corresponds with one row of culture dish of below, and strip cloth sap cavity bottom sets up a plurality of and pours into the culture solution discharge pipe in each culture dish with the culture solution of strip cloth sap cavity into, pumping mechanism include a plurality of miniature liquid pump, every miniature liquid pump corresponds a strip cloth sap cavity, and the liquid suction pipe of miniature liquid pump stretches into culture solution storage intracavity and extracts the culture solution to strip cloth sap cavity is arrived in the pumping.
As another optimization scheme of the sputum sample pretreatment device for medical examination, a supporting piece surrounding the sampler is arranged in the containing cavity, the supporting piece is of a cavity structure, the outer side wall of the supporting piece is provided with a limiting cylinder in an inverted round platform shape, the limiting cylinder is matched with the outer wall of the sputum taking cup to realize the positioning of the sputum taking cup, and a cavity in the supporting piece forms an installing cavity of equipment or a pipeline.
As another optimized scheme of the sputum sample pretreatment device for medical examination, the sputum taking cup placing seat comprises a limiting pedestal, and the limiting pedestal is matched with a limiting pedestal of another sputum taking cup placing seat to form an accommodating cavity; the injector comprises a compound enzymatic hydrolysate storage cavity and a piston cavity, wherein the compound enzymatic hydrolysate storage cavity is arranged in the limiting pedestal, the piston cavity is provided with a piston, and the bottom of the piston cavity is communicated with the compound enzymatic hydrolysate storage cavity through a pipette with a one-way valve so as to suck the compound enzymatic hydrolysate in the compound enzymatic hydrolysate storage cavity into the piston cavity when the piston is drawn out; bottom one side in piston chamber is provided with the protrusion in the fluid-discharge tube that holds the chamber lateral wall, and when getting the sputum cup and arranging the in-process that holds the chamber in, the tip of fluid-discharge tube pierces through in the disposable injection hole on the cup stretches into the cup to when pushing down the piston, in impressing the compound enzyme solution of piston intracavity into the cup.
As another optimized scheme of the sputum sample pretreatment device for medical examination, the top end of the piston extends out of the piston cavity to form a clamping joint, the clamping joint is in butt joint with a hole in an extension part of a sealing cover on the cup body, and when the sputum taking cup is placed in the accommodating cavity, the extension part drives the clamping joint and then pushes the piston downwards, so that the complex enzyme-hydrolyzed liquid in the piston cavity is extruded into the cup body and mixed with the sputum sample for pretreatment; and a sealing plug is arranged at the top end of the piston cavity.
Compared with the prior art, the invention has the following beneficial effects:
1) the sputum sample pretreatment device can treat the sputum sample, so that bacteria in the sputum sample can be propagated in the queuing inspection process and can be detected more easily, the detection time of the sputum sample is greatly shortened, the detection efficiency is improved, and more importantly, trace pathogenic bacteria in the sputum sample can be detected more accurately; the sampler can puncture the bottom of the sputum taking cup when the sputum taking cup is placed in the sputum taking cup, so that diluted sputum after enzymolysis reaction is dripped into the bacteria culture mechanism for culture after passing through a through channel in the sputum taking cup, and a filter disc is arranged in the through channel and can filter impurities such as floating dust in a sputum sample;
2) the edge of the top end of the sampler is provided with the groove, the groove and the side wall form the scraper, and the scraper can be matched with the bottom of the sputum taking cup to scrape a sputum sample into the groove, so that the sputum sample can more conveniently flow into the through channel; because the sampler is fixed, after the sputum sample of one patient is processed, germs can remain in a through channel, and the sampler is not only unsanitary, but also can influence the precision of the next detection; in order to solve the problem, the sampler is arranged to be metal, a sterilization cleaning unit is arranged in the sampler, the core of the sterilization cleaning unit is an electric heating wire for heating the sampler and a cleaning pipeline capable of spraying water for cleaning, when sterilization cleaning is needed, the electric heating wire is heated, the sampler is heated to more than 80 ℃, such as 100 ℃, and the temperature is maintained for a certain time, such as 30s, in the process, residual germs in the sampler can be effectively killed, then a cleaning water pump is opened, cleaning water enters the sampler along the cleaning pipeline, the sampler is thoroughly cleaned through a through channel, and after cleaning is finished, the electric heating wire is opened again for drying, so that self-cleaning of the sampler is conveniently and quickly realized;
3) the sputum taking cup is provided with the disposable injection hole, so that the composite enzymatic hydrolysate for treating the sputum sample can be injected into the sputum taking cup in a closed state with the sputum sample, meanwhile, the bottom of the sputum taking cup is provided with the flow guide pipe matched with the sampler, and the bottom end of the flow guide pipe is closed by the thin film, so that the obtained sputum sample is preferentially gathered into the flow guide pipe under the action of gravity, and after the thin film is punctured by the sampler, the sputum sample in the flow guide pipe can be automatically gathered to the top end of the sampler;
4) the sputum sample pretreatment device can be matched with the sputum taking cup to treat a plurality of sputum samples at one time, and can automatically realize the injection of compound enzymatic hydrolysate in the process of putting the sputum taking cup, and automatically fall into a culture dish filled with culture solution for propagation after reacting for a period of time when the sputum samples become thin, thereby shortening the treatment and culture time during detection, and enabling trace pathogenic bacteria possibly contained in the sputum samples to propagate and be more easily detected.
Drawings
FIG. 1 is a schematic view of a sputum sample pretreatment apparatus of the present invention in use;
FIG. 2 is a schematic view of a sputum sample pretreatment apparatus according to the present invention;
FIG. 3 is a schematic diagram showing the arrangement of the sputum cup holder in the sputum sample pretreatment apparatus;
FIG. 4 is a schematic view of the structure of the sputum cup;
FIG. 5 is a schematic diagram of the structure of the sampler;
FIG. 6 is a schematic view of the sputum cup holder with the syringe not depressed;
FIG. 7 is a schematic view of the sputum cup holder with the injector pushed down;
FIG. 8 is a schematic view of the connection of the culture dish;
reference numerals: 1. a shell, 101, a sputum-taking cup placing cavity, 102, a culture dish taking and placing cavity, 103, a culture solution storage cavity, 104, a cover plate, 105, a spare part box, 106, an equipment cavity, 2, a sputum-taking cup, 201, a cup body, 202, a flow guide pipe, 203, a film, 204, a disposable injection hole, 205, a sealing cover, 206, an extension part, 3, a sputum-taking cup placing seat, 301, a limiting seat, 302, an accommodating cavity, 303, a limiting cylinder, 304, a support piece, 4, a bacteria culture mechanism, 401, a culture dish, 402, a track, 403, a heat preservation cavity, 404, a connecting piece, 405, a handle part, 5, an injector, 501, a piston cavity, 502, a piston, 503, a clamping head, 504, a composite enzymatic liquid discharge pipe, 505, a disposable puncture tube, 506, a sealing plug, 507, 508, a complex enzymatic liquid storage cavity, 6, a pumping mechanism, 601, a micro liquid pump, 602, a liquid suction pipe, 603, a strip-shaped liquid distribution cavity, 604 and a culture solution discharge pipe, 7. the device comprises a sterilization cleaning unit 701, a main cleaning pipeline 702, a branch cleaning pipeline 703, a cleaning hole 704, an electric heating wire 8, a sampler 801, a cylinder 802, a conical tip 803, a groove 804, a scraper 805, a through channel 806, a filter plate 807 and an annular wedge.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the following specific embodiments. The invention is not illustrated in the following examples, which are prior art.
Example 1
As shown in fig. 1, 2 and 5, a sputum sample pretreatment device for medical examination comprises a housing 1 and a plurality of sputum taking cup placement seats 3 arranged in the housing 1, wherein each sputum taking cup placement seat 3 is internally provided with a containing cavity 302 for placing a sputum taking cup 2, an injector 5 for injecting composite enzymolysis liquid into the sputum taking cup 2 to pretreat the sputum sample in the sputum taking cup 2, and a sampler 8 for penetrating the bottom of the sputum taking cup 2 and guiding the pretreated sputum sample out of a bacterial culture mechanism 4 pre-filled with culture solution to culture and propagate; the sampler 8 includes that the top has the cylinder 801 that penetrates the sharp-pointed 802 of toper in the phlegm cup 2 of getting, offers two at least confession through passageway 805 that the phlegm sample after the preliminary treatment passed along the axial of cylinder 801, all is provided with in each through passageway 805 and carries out filterable cassette 806 to the phlegm sample.
The compound enzymatic hydrolysate used in the embodiment is the compound enzymatic hydrolysate used in the existing clinical laboratory, and the main purpose is to carry out the enzymolysis of protein in a sputum sample in advance to make the sputum sample break away from a viscous state, wherein contained germs can flow into the bacteria culture mechanism 4 along with the sputum sample which becomes liquid, and because the filter plate 806 is arranged in the sampler 8, the bacteria can further filter impurities such as dust in the sputum sample, and the like, so that the bacteria are dripped into the bacteria culture mechanism 4, and the propagation is expanded in the culture solution, so that trace pathogenic bacteria can be detected more easily.
In this embodiment, sampler 8 is not disposable, so its connected mode adopts and to dismantle the connection to can wholly take off and wash and install again after the disinfection and isolation and use.
The foregoing is a basic embodiment of the present invention, and further modifications, optimizations and limitations can be made on the foregoing, so as to obtain the following examples:
example 2
The present embodiment is an optimized scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in FIG. 5, the center of the bottom of the tapered tip 802 protrudes from the top of the cylinder 801 through a connector, and the top of the cylinder 801 is provided with a groove 803 surrounding the connector, the edge of the groove 803 and the sidewall of the cylinder 801 form a scraper 804 surrounding the tapered tip 802, and the scraper 804 and the bottom of the tapered tip 802 form an opening.
Example 3
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 5, the sampler 8 has a sterilization and washing unit 7 for washing and sterilizing the sampler, and the sterilization and washing unit 7 includes a main washing line 701 communicated with a washing water pump disposed in the equipment chamber 106 at the lower part of the casing 1, a branch washing line 702 connected to the main washing line 701 and feeding washing water into each sampler 8, and an electric heating wire 704 disposed in the sampler 8 for heating itself; the electric heating wire 704 is arranged in a closed cavity which is arranged in the center of the column body 801 and extends along the axial direction of the column body, the through channel 805 is arranged around the closed cavity, a cleaning hole 703 is arranged in the center of the bottom of the column body 801, the tail end of the cleaning hole 703 is communicated with the bottom end of the through channel 805, and the head end of the cleaning hole 703 is communicated with the branch cleaning pipeline 702, so that cleaning water enters the through channel 805 under certain pressure to clean the through channel 805; the filter plate 806 is a thin sheet woven by metal wires and provided with slits, and is arranged in the through channel 805 in a sliding manner, and the upper surface of the filter plate 806 is connected to the bottom of an annular wedge 807 arranged on the inner wall of the top end of the through channel 805 through a tension spring.
In this embodiment, the annular wedge 807 has a right triangle cross section, and the thickness of the annular wedge 807 gradually increases from the top end to the bottom end of the through passage 805, so that a flat surface for connecting the tension spring is formed at the bottom thereof.
In this embodiment, owing to need not pull down sampler 8 wholly and wash poststerilization, adopt the mode of heating sterilization, metal is chooseed for use to sampler 8's material to can bear high temperature.
Example 4
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 4, the sputum taking cup 2 has a conical cup body 201, a sealing cover 205 is arranged on the top of the cup body 201, a flow guide tube 202 matched with the sampler 8 is arranged on the bottom of the cup body, the bottom end opening of the flow guide tube 202 is sealed by a film 203, and when the sputum taking cup 2 is placed in the sputum taking cup placing seat 3, the top end of the sampler 8 punctures the film 203; a disposable injection hole 204 for the injector 5 to pierce and penetrate into is formed on the upper side wall of the cup body 201.
In this embodiment, the sputum cup 2 is a disposable plastic cup made of medical plastic, and the sealing cap 205 on the top is sealed after the sputum sample is filled with the bacteria to escape into the air; because the sputum is generally more viscous, the existence of the flow guide tube 202 can enable the sputum sample to naturally gather in the flow guide tube and can be matched with the cylindrical sampler 8, and after the top end of the sampler 8 punctures the film 203 at the bottom of the sampler, the sputum sample is easier to gather at the top end of the sampler 8; the disposable injection hole 204 on the upper side wall of the cup body 201 is intact when taking the sputum sample, so as to form a closed space by matching with the film 203 and the sealing cover 205, and prevent germs in the sputum sample from escaping into the air; the disposable injection hole 204 is easily punctured to facilitate injection of an enzymatic solution thereto for pretreatment of the sputum sample.
Example 5
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 1 and 2, the inside of the housing 1 is divided into a sputum cup placing cavity 101, a culture dish taking and placing cavity 102, a culture solution storage cavity 103 and a spare part box 105 from top to bottom, wherein the sputum cup placing seat 3 is arranged in the sputum cup placing cavity 101, an opening which can be opened and is sealed by a cover plate 104 is arranged at the top of the sputum cup placing cavity 101, and the sputum cup 2 is placed in or taken out through the opening; the bacterial culture mechanism 4 is arranged in the culture dish taking and placing cavity 102, and the side wall of the culture dish taking and placing cavity 102 is provided with an openable window so as to take out or place a culture dish 401, which is pre-filled with culture solution, from the bacterial culture mechanism 4 through the window; each culture dish taking and placing cavity 102 is positioned below one sampler 8; the culture solution storage cavity 103 stores culture solution, and the culture solution is conveyed to each culture dish 401 through the pumping mechanism 6; the spare part container 105 is an openable cavity in which a spare culture dish 401 and a sputum cup 2 are stored.
Example 6
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 3, 6, 7 and 8, a plurality of rows of sputum-taking cup holders 3 in the housing 1 are regularly distributed along the direction of a window of the culture dish taking and placing cavity 102, the culture dishes 401 below each row of sputum-taking cup holders 3 are connected into a whole through a connecting piece 404, and extend out of the window through a handle part 405, so that the culture dishes 401 can be pulled out or pushed in through the handle part 405, two sides of the culture dishes 401 are provided with tracks 402 along the direction of pulling out or pushing in the culture dishes 401, and a heat preservation cavity 403 is arranged below the culture dishes 401 for preserving the temperature in the culture dishes 401.
In this embodiment, the heat preservation chamber 403 has a constant temperature heating wire therein; the culture dish 401 is internally sleeved with a disposable plastic culture dish, thereby being convenient for replacement.
Example 7
The present embodiment is an optimized scheme based on embodiment 6, and the main structure of the present embodiment is the same as that of embodiment 6, and the improvement point is that: as shown in fig. 6 and 7, the top of the culture dish taking and placing cavity 102 has a plurality of strip-shaped liquid distribution cavities 603, each strip-shaped liquid distribution cavity 603 corresponds to one row of culture dishes 401 below, the bottom of the strip-shaped liquid distribution cavity 603 is provided with a plurality of culture liquid discharge pipes 604 for injecting the culture liquid in the strip-shaped liquid distribution cavity 603 into each culture dish 401, the pumping mechanism 6 comprises a plurality of micro liquid pumps 601, each micro liquid pump 601 corresponds to one strip-shaped liquid distribution cavity 603, and the liquid suction pipe 602 of the micro liquid pump 601 extends into the culture liquid storage cavity 103 to draw the culture liquid and pumps the culture liquid into the strip-shaped liquid distribution cavity 603.
Example 8
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 6 and 7, a supporting member 304 surrounding the sampler 8 is arranged in the accommodating cavity 302, the supporting member 304 is of a hollow cavity structure, the outer side wall of the supporting member 304 is provided with a reverse truncated cone-shaped limiting cylinder 303, the limiting cylinder 303 is matched with the outer wall of the sputum taking cup 2 to position the sputum taking cup 2, and the hollow cavity in the supporting member 304 forms a mounting cavity for equipment or a pipeline.
Example 9
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 6 and 7, the sputum cup placing seat 3 comprises a limit seat 301, and the limit seat 301 is matched with the limit seat 301 of another sputum cup placing seat 3 to form an accommodating cavity 302; the injector 5 comprises a compound enzymatic hydrolysate storage cavity 508 arranged in the limiting pedestal 301 and a piston cavity 501 with a piston 502, wherein the bottom of the piston cavity 501 is communicated with the compound enzymatic hydrolysate storage cavity 508 through a pipette 507 with a one-way valve, so that when the piston 502 is drawn out, the compound enzymatic hydrolysate in the compound enzymatic hydrolysate storage cavity 508 is sucked into the piston cavity 501; a liquid discharge pipe 504 protruding out of the side wall of the accommodating cavity 302 is arranged on one side of the bottom of the piston cavity 501, and when the sputum taking cup 2 is placed in the accommodating cavity 302, the end of the liquid discharge pipe 504 pierces the disposable injection hole 206 on the cup body 201 and extends into the cup body 201, and when the piston 502 is pressed down, the composite enzymolysis liquid in the piston cavity 501 is pressed into the cup body 201.
Example 10
The present embodiment is an optimized solution based on embodiment 9, and the main structure thereof is the same as that of embodiment 9, and the improvement point is that: as shown in fig. 6 and 7, the top end of the piston 502 extends out of the piston cavity 501 to form a clamping joint 503, the clamping joint 503 is in butt joint with a hole on the extension 206 of the sealing cover 205 on the cup body 201, and when the sputum taking cup 2 is placed into the accommodating cavity 302, the extension 206 drives the clamping joint 503 to push the piston 502 downwards, so that the composite enzymatic hydrolysate in the piston cavity 501 is squeezed into the cup body 201, and is mixed with the sputum sample for pretreatment; the top end of the piston cavity 501 is provided with a sealing plug 506.
Example 11
The present embodiment is another optimization scheme based on embodiment 9, and the main structure of the present embodiment is the same as that of embodiment 9, and the improvement point is that: as shown in figures 6 and 7, a disposable puncture tube 505 is detachably connected to the free end of the drainage tube 504, and the disposable puncture tube 505 punctures the disposable injection hole 206 and enters the cup body 201 during the process of placing the sputum cup 2 into the containing cavity 302.
In this embodiment, the end of the disposable puncture tube 505 is a slant surface and gradually retracts from the upper edge to the lower edge of the end, so that the upper edge pierces the sealing cap of the disposable injection hole 206 and a gap is formed between the lower edge and the sealing cap, thereby facilitating the composite enzymatic hydrolysate to flow into the cup body 201.

Claims (10)

1. The utility model provides a medical science inspection is with phlegm sample preprocessing device which characterized in that: the device comprises a shell (1) and a plurality of sputum taking cup arranging seats (3) arranged in the shell (1), wherein each sputum taking cup arranging seat (3) is internally provided with a containing cavity (302) for arranging a sputum taking cup (2), an injector (5) for injecting compound enzymatic hydrolysate into the sputum taking cup (2) to pretreat a sputum sample in the sputum taking cup (2), and a sampler (8) which penetrates into the bottom of the sputum taking cup (2) and guides the pretreated sputum sample out of a bacteria culture mechanism (4) pre-filled with culture solution to culture and propagate; sampler (8) have the cylinder (801) that penetrates the sharp-pointed end of toper (802) in getting phlegm cup (2) including the top, offer two at least confession through passageway (805) that the phlegm sample after the preliminary treatment passed along the axial of cylinder (801), all be provided with in each through passageway (805) and carry out filterable cassette (806) to the phlegm sample.
2. The sputum sample pretreatment apparatus for medical examination as claimed in claim 1, wherein: the center of the bottom of the conical tip (802) protrudes out of the top end of the cylinder (801) through a connecting piece, the top end of the cylinder (801) is provided with a groove (803) surrounding the connecting piece, the edge of the groove (803) and the side wall of the cylinder (801) form a scraper (804) surrounding the conical tip (802), and the scraper (804) and the bottom of the conical tip (802) form an opening.
3. The sputum sample pretreatment apparatus for medical examination as claimed in claim 1, wherein: the sampler (8) is internally provided with a sterilization cleaning unit (7) for cleaning and sterilizing the sampler, the sterilization cleaning unit (7) comprises a main cleaning pipeline (701) communicated with a cleaning water pump, a branch cleaning pipeline (702) connected with the main cleaning pipeline (701) and used for sending cleaning water into each sampler (8), and an electric heating wire (704) arranged in the sampler (8) and used for heating the sampler, wherein the cleaning water pump is arranged in an equipment cavity (106) at the lower part of the shell (1); the electric heating wire (704) is arranged in a closed cavity which is arranged in the center of the column body (801) and extends along the axial direction of the column body, the through channel (805) is arranged around the closed cavity, a cleaning hole (703) is arranged in the center of the bottom of the column body (801), the tail end of the cleaning hole is communicated with the bottom end of the through channel (805), and the head end of the cleaning hole (703) is communicated with the branch cleaning pipeline (702), so that cleaning water enters the through channel (805) at a certain pressure to clean the through channel; the filter plate (806) is a thin sheet with gaps formed by weaving metal wires and is arranged in the through channel (805) in a sliding mode, and the upper surface of the filter plate (806) is connected to the bottom of an annular wedge (807) arranged on the inner wall of the top end of the through channel (805) through a tension spring.
4. The sputum sample pretreatment apparatus for medical examination as claimed in claim 1, wherein: the sputum taking cup (2) is provided with a conical cup body (201), the top of the cup body (201) is provided with a sealing cover (205), the bottom of the cup body is provided with a flow guide pipe (202) matched with the sampler (8), the bottom end opening of the flow guide pipe (202) is sealed by a thin film (203), and when the sputum taking cup (2) is placed in the sputum taking cup placing seat (3), the top end of the sampler (8) punctures the thin film (203); the side wall of the upper part of the cup body (201) is provided with a disposable injection hole (204) for the injector (5) to puncture and penetrate into.
5. The sputum sample pretreatment apparatus for medical examination as claimed in claim 1, wherein: the interior of the shell (1) is divided into a sputum taking cup placing cavity (101), a culture dish taking and placing cavity (102), a culture solution storage cavity (103) and a spare part box (105) from top to bottom, wherein the sputum taking cup placing seat (3) is arranged in the sputum taking cup placing cavity (101), the top of the sputum taking cup placing cavity (101) is provided with an opening which can be opened and is sealed by a cover plate (104), and the sputum taking cup (2) is placed in or taken out through the opening; the bacterial culture mechanism (4) is arranged in the culture dish taking and placing cavity (102), and the side wall of the culture dish taking and placing cavity (102) is provided with an openable window so as to take out or place a culture dish (401) pre-filled with culture solution in the bacterial culture mechanism (4) through the window; each culture dish taking and placing cavity (102) is positioned below one sampler (8); the culture solution storage cavity (103) stores culture solution, and the culture solution is conveyed to each culture dish (401) through a pumping mechanism (6); the spare part box (105) is an openable cavity, and a spare culture dish (401) and a sputum taking cup (2) are stored in the spare part box.
6. The sputum sample pretreatment apparatus for medical examination as claimed in claim 1, wherein: the utility model discloses a sputum collection device, including casing (1), get the window direction law distribution a plurality of rows that phlegm cup mount pad (3) were put along the culture dish in casing (1), culture dish (401) of every row of phlegm cup mount pad (3) below are connected as an organic wholely through connecting piece (404), and extend the window through handle portion (405), be convenient for pull out or push culture dish (401) through handle portion (405), the both sides of culture dish (401) have along track (402) of culture dish (401) extraction or push-in direction, below in culture dish (401) is provided with heat preservation chamber (403), a temperature for in keeping culture dish (401).
7. The sputum sample pretreatment apparatus for medical examination as claimed in claim 6, wherein: the top that the chamber (102) was got to the culture dish has a plurality of strip cloth liquid chamber (603), and each strip cloth liquid chamber (603) corresponds with one row of culture dish (401) of below, and strip cloth liquid chamber (603) bottom sets up a plurality of culture solution discharge pipe (604) of pouring into each culture dish (401) with the culture solution in strip cloth liquid chamber (603), pumping mechanism (6) include a plurality of miniature fluid pump (601), every miniature fluid pump (601) correspond a strip cloth liquid chamber (603), and draw tube (602) of miniature fluid pump (601) stretch into extract culture solution in culture solution storage chamber (103) to the pumping is to in strip cloth liquid chamber (603).
8. The sputum sample pretreatment apparatus for medical examination as claimed in claim 1, wherein: the utility model discloses a sputum sampling device, including holding chamber (302), be provided with support piece (304) around sampler (8) in holding chamber (302), this support piece (304) is inside to be the cavity structure, and its lateral wall is provided with spacing section of thick bamboo (303) of the form of round of falling platform, and this spacing section of thick bamboo (303) and the outer wall cooperation of getting phlegm cup (2), realize getting the location of phlegm cup (2), and the cavity in support piece (304) forms and settles equipment or pipeline installation chamber.
9. The sputum sample pretreatment apparatus for medical examination as claimed in claim 1, wherein: the sputum taking cup placing seat (3) comprises a limiting seat (301), and the limiting seat (301) is matched with the limiting seat (301) of the other sputum taking cup placing seat (3) to form an accommodating cavity (302); the injector (5) comprises a composite enzymatic hydrolysate storage cavity (508) arranged in the limiting pedestal (301) and a piston cavity (501) with a piston (502), wherein the bottom of the piston cavity (501) is communicated with the composite enzymatic hydrolysate storage cavity (508) through a pipette (507) with a one-way valve, so that when the piston (502) is drawn out, the composite enzymatic hydrolysate in the composite enzymatic hydrolysate storage cavity (508) is sucked into the piston cavity (501); a liquid discharge pipe (504) protruding out of the side wall of the accommodating cavity (302) is arranged on one side of the bottom of the piston cavity (501), and when the sputum taking cup (2) is placed in the accommodating cavity (302), the end of the liquid discharge pipe (504) pierces through the disposable injection hole (206) in the cup body (201) and extends into the cup body (201), and when the piston (502) is pressed down, the composite enzymolysis liquid in the piston cavity (501) is pressed into the cup body (201).
10. The sputum sample pretreatment apparatus for medical examination as claimed in claim 9, wherein: the top end of the piston (502) extends out of the piston cavity (501) to form a clamping joint (503), the clamping joint (503) is in butt joint with a hole in an extension part (206) of a sealing cover (205) on the cup body (201), and when the sputum taking cup (2) is placed into the accommodating cavity (302), the extension part (206) drives the clamping joint (503) to push the piston (502) downwards, so that the composite enzymatic hydrolysate in the piston cavity (501) is extruded into the cup body (201) and mixed with a sputum sample for pretreatment; the top end of the piston cavity (501) is provided with a sealing plug (506).
CN202110470887.0A 2021-04-29 2021-04-29 Medical science inspection is with phlegm sample preprocessing device Active CN113188867B (en)

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