CN113188866A - Preprocessing device that pathogenic bacteria used in gastroenterology inspection fecal sample - Google Patents

Preprocessing device that pathogenic bacteria used in gastroenterology inspection fecal sample Download PDF

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CN113188866A
CN113188866A CN202110470881.3A CN202110470881A CN113188866A CN 113188866 A CN113188866 A CN 113188866A CN 202110470881 A CN202110470881 A CN 202110470881A CN 113188866 A CN113188866 A CN 113188866A
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sample
sample processing
cylinder
pathogenic bacteria
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CN113188866B (en
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郑玉峰
王瑞芳
田笑笑
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First Affiliated Hospital of Henan University of Science and Technology
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    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/02Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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    • G01MEASURING; TESTING
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    • G01N1/00Sampling; Preparing specimens for investigation
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088Concentrating samples by other techniques involving separation of suspended solids filtration

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Abstract

一种消化科检验粪便样本中致病菌用的预处理装置,包括壳体以及设置在壳体内可打开的样本处理腔,粪便样本放置在密封的一次性取样杯内,送入到样本处理腔内固定后进行前处理,样本处理腔内设置有可升降的样本处理探头,样本处理探头包括底部具有锥形尖端的柱体,在该柱体内具有与处理液输入管连通的处理液管路,壳体内设置有异味抽吸机构。本发明能够使粪便样本中的微量细菌在检验之前被扩繁,更加容易被检测出来,不仅大大缩短了粪便样本的检测时间,提高了检测效率,更主要的是,能够更加精确检测出其中含有的微量致病菌,提前检测出疾病,避免延误治疗时机,而异味抽吸机构能够抽走粪便样本处理过程中的臭味,保持空气清洁。

Figure 202110470881

A pretreatment device for the examination of pathogenic bacteria in fecal samples in a gastroenterology department, comprising a casing and an openable sample processing cavity arranged in the casing, the fecal samples are placed in a sealed disposable sampling cup, and sent to the sample processing cavity After internal fixation, pretreatment is performed. A sample processing probe that can be raised and lowered is arranged in the sample processing chamber. The sample processing probe includes a cylinder with a tapered tip at the bottom, and a processing liquid pipeline connected to the processing liquid input pipe is arranged in the cylinder. An odor suction mechanism is arranged in the casing. The invention can make the micro bacteria in the fecal sample propagate before the inspection, and it is easier to be detected, not only greatly shortens the detection time of the fecal sample, but also improves the detection efficiency, and more importantly, it can more accurately detect the bacteria contained in the fecal sample. The trace pathogenic bacteria can be detected in advance to avoid delays in treatment, and the odor suction mechanism can remove the odor during the processing of fecal samples and keep the air clean.

Figure 202110470881

Description

Preprocessing device that pathogenic bacteria used in gastroenterology inspection fecal sample
Technical Field
The invention relates to the field of testing of stool samples in the gastroenterology department, in particular to a pretreatment device for testing pathogenic bacteria in the stool samples in the gastroenterology department.
Background
Since a large amount of bacteria can be seen in feces to reflect the pathological changes of the digestive system of a human body, a patient is often required to provide a feces sample for examination during disease diagnosis in the gastroenterology department, and whether a certain disease is suffered or not is judged according to the type of flora in the feces sample, for example, the ratio of cocci to bacilli in normal feces is about 1:10, human yeast can be found, candida is rarely found, candida albicans can be found when intestinal flora imbalance occurs, and part of characteristic bacteria or pathogenic bacteria can be discharged along with feces when some special diseases occur in the intestinal tract.
The existing bacterial examination of the digestive department excrement sample is generally microscopic examination, a fresh patient excrement sample is adopted, but if the bacterial examination is in the early stage of pathological changes, pathogenic bacteria or characteristic bacteria in the excrement sample are possibly few, and the bacterial examination is easy to die in the queuing inspection process, so that the bacterial examination is difficult to examine, and the optimal treatment time is delayed.
Disclosure of Invention
In order to solve the problem that pathogenic bacteria or characteristic bacteria in early disease are low in content and difficult to detect, the invention provides a pretreatment device for detecting the pathogenic bacteria in a fecal sample in the gastroenterology department.
The technical scheme adopted by the invention for solving the technical problems is as follows: a pretreatment device for pathogenic bacteria in a digestive department test stool sample comprises a shell and an openable sample treatment cavity arranged in the shell, wherein the stool sample is placed in a sealed disposable sampling cup and is sent into the sample treatment cavity for fixation and then is pretreated; a liftable sample processing probe is arranged in the sample processing cavity, the sample processing probe comprises a cylinder with a conical tip at the bottom, a processing liquid pipeline communicated with a processing liquid input pipe is arranged in the cylinder, and after the excrement sample in the disposable sampling cup is inserted into the bottom end of the sample processing probe, the processing liquid is injected into the excrement sample through the processing liquid pipeline;
the peculiar smell suction mechanism is arranged in the shell and comprises a gas filtering and absorbing cavity which is arranged in the shell and filled with an activated carbon adsorption bag, the gas filtering and absorbing cavity is communicated with the inside of the sample treatment cavity through air holes densely distributed on the surface of the gas filtering and absorbing cavity and is communicated with a negative pressure suction pipeline after extending out of the shell through a secondary absorption pipe, and the secondary absorption pipe is filled with a zeolite molecular sieve, so that the gas in the sample treatment cavity is discharged through the negative pressure suction pipeline after being purified by the activated carbon adsorption bag and the zeolite molecular sieve in sequence.
As another optimized scheme of the pretreatment device for pathogenic bacteria in the digestive department test stool samples, a bottom plate of the sample treatment cavity is enclosed by the inner wall of the shell, a truncated cone-shaped fixed cavity is formed by downwards sinking the center of the bottom plate, the inner wall of the fixed cavity is attached to the side wall and the bottom wall of the disposable sampling cup, and the disposable sampling cup filled with the stool samples is fixed in the fixed cavity by bolts; the center of the bottom of the fixed cavity is provided with a penetrating hole for the fluid to pass through after penetrating through the disposable sampling cup; the bottom plate below has a backup pad, and the lateral wall of fixed chamber bottom is connected to this backup pad to form the gas filtration absorption chamber that encircles fixed chamber, all densely covered the gas pocket on backup pad and the bottom plate.
As another optimization scheme of the pretreatment device for pathogenic bacteria in the excrement sample for the gastroenterology examination, a cylindrical cavity with an opening at the bottom end is arranged in the center of the cylinder, a flow guide body is arranged in the cylindrical cavity in a sliding mode, a hydraulic driving cavity is formed between the top of the flow guide body and the top of the cylindrical cavity and communicated with a first hydraulic input pipe, so that the conical tip of the flow guide body is controlled to extend out of or retract into the cylindrical cavity by utilizing hydraulic oil, the bottom of the disposable sampling cup is punctured when the conical tip of the flow guide body extends out, and the excrement sample treated by the treatment liquid is dripped into a culture dish of a bacteria culture mechanism below the sample treatment cavity for bacteria culture.
As another optimization scheme of the pretreatment device for pathogenic bacteria in the digestive system test fecal samples, a drainage channel is arranged in the flow guide body, the bottom end opening of the drainage channel is arranged on the bottom end surface of the flow guide body, the top end of the drainage channel is connected with a plurality of drainage holes which obliquely extend upwards to the side wall openings of the drainage channels, and the drainage holes are communicated with the liquid inlet holes formed in the side wall of the bottom end of the column body in a one-to-one correspondence manner when the flow guide body extends to the limit position, so that the treated fecal sample liquid is dripped into the culture dish through the liquid inlet holes, the drainage holes and the drainage channel.
As another optimization scheme of the pretreatment device for detecting pathogenic bacteria in the excrement sample in the gastroenterology department, the liquid inlet hole is obliquely arranged, one end of the opening with the high position extends to the bottom end of the side face of the column body, one end with the low position is communicated with the column-shaped cavity, the inner diameter of the liquid inlet hole is gradually reduced from one end with the high position to one end with the low position, and the filter screen is arranged in the liquid inlet hole to filter impurities in the excrement sample liquid.
As another optimized scheme of the pretreatment device for pathogenic bacteria in the digestive system test fecal samples, the bacterial culture mechanism is arranged in a culture dish taking and placing cavity of the shell, and the side wall of the culture dish taking and placing cavity is provided with an openable window so as to take out or place a culture dish pre-filled with culture solution in the bacterial culture mechanism through the window; the both sides of culture dish have along the track of culture dish extraction or push-in direction, are provided with the heat preservation chamber in the below of culture dish for keep the temperature in the culture dish.
As another optimized scheme of the pretreatment device for pathogenic bacteria in the digestive system examination fecal sample, a plurality of reversible cutting blades are arranged around the column body, the top ends of the reversible cutting blades are hinged on the side wall of the column body, a columnar telescopic bag is arranged between the middle part of the reversible cutting blades and the column body, the columnar telescopic bag is communicated with a second hydraulic input pipe through a hydraulic hole channel in the column body, and hydraulic oil is injected into or pumped out of the columnar telescopic bag, so that the reversible cutting blades and the column body keep an included angle of 50-80 degrees or are attached to the side wall of the column body;
the cylinder is driven to rotate by a driving mechanism, and is communicated with an external liquid pipeline by utilizing a rotary joint, and the main body of the driving mechanism is a motor.
As another optimization scheme of the pretreatment device for pathogenic bacteria in the digestive department test stool sample, the top end of the sample treatment probe is connected with the driving mechanism, the driving mechanism is arranged on the lifting plate, the edge of the lifting plate is arranged in the vertical sliding groove of the side wall of the shell in a sliding manner, the lifting plate divides the upper part of the sample treatment cavity into a height-variable equipment cavity, and the driving mechanism is driven by four groups of synchronous hydraulic lifting oil cylinders in the equipment cavity to lift, so that the lifting of the sample treatment probe is realized.
As another optimization scheme of the pretreatment device for detecting pathogenic bacteria in the fecal sample in the gastroenterology department, the disposable sampling cup is provided with a truncated cone-shaped cup body, the top and the bottom of the cup body are respectively provided with a film sealing cover and a sealing film, the top of the cup body is provided with a horizontal extension part, and the extension part is matched and fixed with the top end of a fixed cavity of a sample processing cavity.
The treatment liquid is a common treatment liquid for treating excrement in the medical field, mainly aims to dilute an excrement sample and enable bacteria in the excrement to enter the treatment liquid so as to be convenient to check, and comprises a glycerol aqueous solution with the mass fraction of 10-15%.
Compared with the prior art, the invention has the following beneficial effects:
1) the invention can process the excrement sample, so that trace bacteria in the excrement sample are propagated before inspection and are easier to detect, thereby not only greatly shortening the detection time of the excrement sample and improving the detection efficiency, but also more importantly, the invention can more accurately detect trace pathogenic bacteria contained in the excrement sample, detect the disease in advance and avoid delaying the treatment time;
2) when the excrement sample is treated, the excrement sample is in a closed environment, so that peculiar smell of excrement is easily emitted, and the air in an inspection room is abnormal and smelly; in order to solve the problem, the odor suction mechanism is arranged in the shell, the main body of the odor suction mechanism is the activated carbon adsorption bag and the zeolite molecular sieve in the secondary adsorption pipe, and in the process of sucking air in the shell by utilizing negative pressure, gas is firstly adsorbed by the activated carbon adsorption bag for the first time and then absorbs odor for the second time through the zeolite molecular sieve in the secondary adsorption pipe, so that the odor is prevented from being emitted, and the environment of an inspection room is improved;
3) the sample processing probe can be inserted into the disposable sampling cup, the treatment liquid is injected into the excrement sample to dilute excrement, then the flow guide body in the sample processing probe can extend out to puncture the bottom of the disposable sampling cup again, and the diluted excrement sample is introduced into the bacterial culture mechanism to be cultured, so that trace pathogenic bacteria can be propagated, the quantity of the pathogenic bacteria is increased, and the pathogenic bacteria can be easily detected in the later period;
4) the guide body of the sample processing probe can extend out or retract into the column body of the processing probe under the hydraulic drive, so that the guide body can retract into the column body when the excrement sample is diluted, the bottom end of the processing probe is contacted with the excrement sample as much as possible, and the processing liquid is released, so that bacteria in excrement can enter the processing liquid to complete the processing; then the flow guide body extends out and punctures the bottom of the disposable sampling cup, so that the treatment liquid containing bacteria can pass through the liquid inlet hole on the side surface of the column body and drop into a culture dish along the flow guide channel in the flow guide body for culture, and at the moment, the excrement sample soaked by the treatment liquid is still in the disposable sampling cup and is convenient to take out; in order to enable bacteria in excrement to enter the treatment liquid as much as possible, the treatment probe is further provided with the turnable cutting blades, the turnable cutting blades can keep a certain angle with the cylinder of the treatment probe under the control of the columnar telescopic bag, and meanwhile, the cylinder rotates to cut and stir the excrement sample, so that the treatment liquid can contact the excrement sample as much as possible, and further, the bacteria in the treatment liquid can enter the treatment liquid.
Drawings
FIG. 1 is a schematic view of the internal structure of the present invention;
FIG. 2 is a schematic view after being loaded into a disposable sampling cup;
FIG. 3 is a schematic view of a treatment probe extended into a disposable sampling cup;
FIG. 4 is a schematic view of the treatment probe with the reversible cutting blades deployed;
FIG. 5 is a schematic view of the fluid carrier extending out after puncturing the disposable sampling cup;
FIG. 6 is a schematic view of the interior of the housing of the present invention;
FIG. 7 is a schematic view of a disposable sampling cup;
FIG. 8 is a schematic view of a treatment probe;
FIG. 9 is an enlarged schematic view of the treatment probe;
FIG. 10 is a schematic view of a treatment probe with a flow conductor extended for drainage;
fig. 11 is a detailed view of the fluid-guiding body and the column body in fig. 10;
reference numerals: 1. a housing 101, a sample processing chamber 102, a lifting plate 103, an apparatus chamber 104, a support plate 105, a fixed chamber 106, a bottom plate 107, a penetration hole 108, a petri dish taking and placing chamber 109, a gas filtration and absorption chamber 2, a driving mechanism 201, a motor 202, a rotary joint 203, a first hydraulic input pipe 204, a processing liquid input pipe 205, a second hydraulic input pipe 206, a synchronous hydraulic lifting cylinder 3, a sample processing probe 301, a column 302, a hydraulic driving chamber 303, a flow guide body 304, a flow guide channel 305, a flow guide hole 306, a liquid inlet hole 307, a processing liquid pipeline 308, a reversible cutting blade 309, a columnar telescopic bag 4, a bacteria culturing mechanism 401, a petri dish 402, a rail 403, a heat preservation chamber 5, a disposable sampling cup 501, a cup body 502, a film cover 503, a sealing film 504, an extension part, 6. peculiar smell suction mechanism 601, active carbon adsorption package, 602, secondary adsorption tube, 603, negative pressure suction pipeline.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the following specific embodiments. The parts of the invention not described in the following embodiments are all the prior art, such as a hydraulic oil cylinder and a hydraulic system and a swivel joint matched with the hydraulic oil cylinder.
Example 1
As shown in fig. 1-5, a pretreatment device for pathogenic bacteria in a stool sample for digestive department examination, which comprises a shell 1 and an openable sample treatment cavity 101 arranged in the shell 1, wherein the stool sample is placed in a sealed disposable sampling cup 5, and is sent into the sample treatment cavity 101 for fixation and then is pretreated; a liftable sample processing probe 3 is arranged in the sample processing cavity 101, the sample processing probe 3 comprises a cylinder 301 with a conical tip at the bottom, a processing liquid pipeline 307 communicated with a processing liquid input pipe 204 is arranged in the cylinder 301, and the processing liquid pipeline 307 injects processing liquid into the excrement sample after the excrement sample in the disposable sampling cup 5 is inserted into the bottom end of the sample processing probe 3;
be provided with peculiar smell suction mechanism 6 in the casing 1, this peculiar smell suction mechanism 6 is including setting up in casing 1 and filling the gas filtration absorption chamber 109 that has activated carbon adsorption package 601, this gas filtration absorption chamber 109 communicates in its surface densely covered gas pocket and sample processing chamber 101 to stretch out casing 1 back through a secondary absorption pipe 602 and communicate with negative pressure suction pipeline 603, be filled with zeolite molecular sieve in secondary absorption pipe 602, so that the gas in sample processing chamber 101 is discharged through negative pressure suction pipeline 603 after activated carbon adsorption package 601 and zeolite molecular sieve purification treatment in proper order.
In this embodiment, the air filtering and absorbing cavity 109 is not communicated with the outside to prevent the odor of the stool sample from escaping; the secondary adsorption pipe 602 is a round pipe, two ends of the secondary adsorption pipe are sealed by breathable gauze, one end of the secondary adsorption pipe is embedded in the activated carbon adsorption bag 601, the other end of the secondary adsorption pipe extends out of the shell 1 and is connected with the negative pressure suction pipeline 603, the negative pressure suction pipeline 603 is connected with the exhaust fan, and in the process of pumping air in the sample processing cavity 109, secondary filtration and purification are carried out on the air, so that peculiar smell emission is removed.
The treatment liquid is a common treatment liquid for treating excrement in the medical field, mainly aims to dilute an excrement sample and enable bacteria in the excrement to enter the treatment liquid so as to be convenient to check, and comprises a glycerol aqueous solution with the mass fraction of 10-15%.
The foregoing is a basic embodiment of the present invention, and further modifications, optimizations and limitations can be made on the foregoing, so as to obtain the following examples:
example 2
The present embodiment is an optimized scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 6, a bottom plate 106 of the sample processing chamber 101 is enclosed with the inner wall of the housing 1, and a truncated cone-shaped fixing chamber 105 is formed by sinking downwards at the center of the bottom plate, the inner wall of the fixing chamber 105 is attached to the side wall and the bottom wall of the disposable sampling cup 5, and the disposable sampling cup 5 filled with the stool sample is fixed in the fixing chamber 105 by bolts; the center of the bottom of the fixed cavity 105 is provided with a penetrating hole 107 for the current guiding body 303 to pass through after penetrating the disposable sampling cup 5; the bottom plate 106 has a support plate 104 below, and the support plate 104 is connected to the sidewall of the bottom of the fixing chamber 105, thereby forming a gas filtering and absorbing chamber 109 surrounding the fixing chamber 105, and the support plate 104 and the bottom plate 106 are densely distributed with air holes.
Example 3
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 8-11, a cylindrical cavity with an open bottom end is provided in the center of the cylinder 301, a flow guiding body 303 is slidably provided in the cylindrical cavity, a hydraulic driving cavity 302 is formed between the top of the flow guiding body 303 and the top of the cylindrical cavity, the hydraulic driving cavity 302 is communicated with the first hydraulic input pipe 203, so that the conical tip of the flow guiding body 303 is controlled to extend out or retract into the cylindrical cavity by using hydraulic oil, and the bottom of the disposable sampling cup 5 is punctured when the conical tip of the flow guiding body 303 extends out, so that the fecal sample treated by the treatment liquid is dripped into a culture dish 401 of the bacteria culture mechanism 4 below the sample treatment cavity 101 for bacteria culture.
In this embodiment, the culture dish 401 contains a culture medium for culturing bacteria, and the culture medium is generally lb medium or agar medium.
Example 4
The present embodiment is an optimized scheme based on embodiment 3, and the main structure of the present embodiment is the same as that of embodiment 3, and the improvement point is that: as shown in fig. 8 and 9, a drainage channel 304 is disposed in the flow guiding body 303, a bottom end of the drainage channel 304 is open on a bottom end surface of the flow guiding body 303, a plurality of drainage holes 305 extending obliquely upward to side wall openings of the drainage channel are connected to a top end of the flow guiding body 303, and when the flow guiding body 303 extends to a limit position, the drainage holes 305 are communicated with liquid inlet holes 306 formed in a bottom end side wall of the cylinder 301 in a one-to-one correspondence manner, so that the processed fecal sample liquid is dropped into the culture dish 401 through the liquid inlet holes 306, the drainage holes 305 and the drainage channel 304.
Example 5
The present embodiment is an optimized scheme based on embodiment 4, and the main structure of the present embodiment is the same as that of embodiment 4, and the improvement point is that: as shown in fig. 9-11, the liquid inlet hole 306 is disposed obliquely, the high open end extends to the bottom end of the side surface of the column 301, the low open end is communicated with the column cavity, the inner diameter of the liquid inlet hole 306 is gradually reduced from the high open end to the low open end, and a filter screen is disposed in the liquid inlet hole 306 to filter impurities in the fecal sample liquid.
Example 6
The present embodiment is another optimization scheme based on embodiment 3, and the main structure of the present embodiment is the same as that of embodiment 3, and the improvement point is that: as shown in fig. 1 to 5, the bacteria culture mechanism 4 is disposed in the culture dish taking and placing cavity 108 of the housing 1, and a side wall of the culture dish taking and placing cavity 108 has an openable window for taking out or placing a culture dish 401 filled with culture solution in the bacteria culture mechanism 4 through the window; both sides of the culture dish 401 have rails 402 along the direction in which the culture dish 401 is drawn out or pushed in, and a soak chamber 403 is provided below the culture dish 401 for maintaining the temperature inside the culture dish 401.
In this embodiment, the culture dish access chamber 108 is sealed except for the openable window to prevent the escape of odor.
In this embodiment, the heat preservation chamber 403 has a constant temperature heating wire therein; the culture dish 401 is internally sleeved with a disposable plastic culture dish, thereby being convenient for replacement.
Example 7
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 8 and 9, a plurality of reversible cutting blades 308 are arranged around the column 301, the top ends of the reversible cutting blades 308 are hinged on the side wall of the column 301, a columnar telescopic bag 309 is arranged between the middle part of the reversible cutting blades 308 and the column 301, the columnar telescopic bag 309 is communicated with the second hydraulic input pipe 205 through a hydraulic hole in the column 301, and the reversible cutting blades 308 and the column 301 keep an included angle of 50-80 degrees or are attached to the side wall of the column 301 by injecting or extracting hydraulic oil into or from the columnar telescopic bag 309;
the cylinder 301 is driven by a driving mechanism 2 to rotate, and is communicated with an external liquid pipeline by using a rotary joint 202, and the main body of the driving mechanism 2 is a motor 201.
In this embodiment, the reversible cutting blades 308 are sharp-edged blades that are uniformly distributed around the cylinder 301, typically 2-4 in number; 2-4 strip-shaped shallow grooves can be uniformly arranged on the surface of the cylinder 301, and a reversible cutting blade 308 is hinged in each strip-shaped shallow groove; the columnar telescopic bag 309 is made of elastic materials, is positioned in the strip-shaped shallow groove, and is vertical to the column body 301 in the telescopic direction.
Example 8
This embodiment is an optimized solution based on embodiment 7, and the main structure thereof is the same as that of embodiment 7, and the improvement point is that: as shown in fig. 1 to 5, the top end of the sample processing probe 3 is connected to a driving mechanism 2, the driving mechanism 2 is disposed on a lifting plate 102, the edge of the lifting plate 102 is slidably disposed in a vertical sliding slot on the side wall of the housing 1, the lifting plate 102 divides the upper portion of the sample processing chamber 101 into a device chamber 103 with a variable height, and the driving mechanism 2 is driven by four sets of synchronous hydraulic lifting cylinders 206 in the device chamber 103 to lift, thereby lifting the sample processing probe 3.
Example 9
The present embodiment is another optimization scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 7, the disposable sampling cup 5 has a truncated cone-shaped cup body 501, the top and bottom of the cup body 501 are respectively provided with a film cover 502 and a sealing film 503, the top of the cup body 501 is provided with a horizontal extension portion 504, and the extension portion 504 is matched and fixed with the top end of the fixing cavity 105 of the sample processing cavity 101.
In this embodiment, the disposable sampling cup 5 is made of plastic, and the top and bottom film covers 502 and the sealing film 503 are provided to prevent odor and bacteria from being emitted, and to facilitate the penetration and penetration of the sample processing probe 3.

Claims (9)

1.一种消化科检验粪便样本中致病菌用的预处理装置,包括壳体(1)以及设置在壳体(1)内可打开的样本处理腔(101),所述粪便样本放置在密封的一次性取样杯(5)内,送入到样本处理腔(101)内固定后进行前处理;其特征在于:所述样本处理腔(101)内设置有可升降的样本处理探头(3),样本处理探头(3)包括底部具有锥形尖端的柱体(301),在该柱体(301)内具有与处理液输入管(204)连通的处理液管路(307),该处理液管路(307)在样本处理探头(3)底端扎入一次性取样杯(5)内的粪便样本后,向粪便样本内注入处理液;1. A pretreatment device for examining pathogenic bacteria in fecal samples in a gastroenterology department, comprising a casing (1) and an openable sample processing cavity (101) disposed in the casing (1), the fecal samples being placed in a The sealed disposable sampling cup (5) is sent into the sample processing chamber (101) to be fixed and then pre-treated; it is characterized in that: the sample processing chamber (101) is provided with a liftable sample processing probe (3). ), the sample processing probe (3) comprises a cylinder (301) with a tapered tip at the bottom, and a processing liquid pipeline (307) communicated with the processing liquid input pipe (204) in the cylinder (301), the processing liquid The liquid pipeline (307) injects the treatment liquid into the stool sample after the bottom end of the sample processing probe (3) is inserted into the stool sample in the disposable sampling cup (5); 所述壳体(1)内设置有异味抽吸机构(6),该异味抽吸机构(6)包括设置在壳体(1)内且填充有活性炭吸附包(601)的气体过滤吸收腔(109),该气体过滤吸收腔(109)通过其表面密布的气孔与样本处理腔(101)内连通,并通过一根二次吸收管(602)伸出壳体(1)后与负压抽吸管路(603)连通,在二次吸附管(602)内填充有沸石分子筛,以使样本处理腔(101)内的气体依次经活性炭吸附包(601)和沸石分子筛净化处理后,通过负压抽吸管路(603)排出。An odor suction mechanism (6) is arranged in the casing (1), and the odor suction mechanism (6) includes a gas filter absorption cavity (6) which is arranged in the casing (1) and is filled with an activated carbon adsorption bag (601). 109), the gas filtration and absorption cavity (109) communicates with the sample processing cavity (101) through the air holes densely covered on its surface, and extends out of the housing (1) through a secondary absorption tube (602) and is pumped with negative pressure. The suction pipe (603) is connected, and the secondary adsorption pipe (602) is filled with zeolite molecular sieve, so that the gas in the sample processing chamber (101) is sequentially purified by the activated carbon adsorption bag (601) and the zeolite molecular sieve, and then passes through the negative Press the suction line (603) to discharge. 2.根据权利要求1所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:与壳体(1)内壁围成所述样本处理腔(101)的底板(106),在其中心向下凹陷形成一个圆台状的固定腔(105),该固定腔(105)的内壁与一次性取样杯(5)的侧壁和底壁贴合,并依靠螺栓将装有粪便样本的一次性取样杯(5)固定在固定腔(105)内;固定腔(105)的底部中心具有供导流体(303)穿透一次性取样杯(5)后通过的穿透孔(107);所述底板(106)下方具有一块支撑板(104),且该支撑板(104)连接固定腔(105)底部的侧壁,从而形成环绕固定腔(105)的气体过滤吸收腔(109),支撑板(104)和底板(106)上均密布气孔。2. A pretreatment device for gastroenterology inspection for pathogenic bacteria in stool samples according to claim 1, characterized in that: a bottom plate ( 106), a truncated cone-shaped fixing cavity (105) is formed in the downward depression in its center, and the inner wall of the fixing cavity (105) is fitted with the side wall and bottom wall of the disposable sampling cup (5), and the fixing cavity (105) is fixed by means of bolts. The disposable sampling cup (5) with the fecal sample is fixed in the fixed cavity (105); the bottom center of the fixed cavity (105) has a penetration hole through which the conducting fluid (303) penetrates the disposable sampling cup (5) (107); a support plate (104) is provided under the bottom plate (106), and the support plate (104) is connected to the side wall of the bottom of the fixing cavity (105), thereby forming a gas filtering and absorbing cavity surrounding the fixing cavity (105) (109), air holes are densely distributed on the support plate (104) and the bottom plate (106). 3.根据权利要求1所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:所述柱体(301)的中心设置有底端开口的柱形腔,在该柱形腔内滑动设置有导流体(303),且该导流体(303)的顶部与柱形腔顶部之间形成液压驱动腔(302),该液压驱动腔(302)与第一液压输入管(203)连通,从而利用液压油控制导流体(303)的锥形尖端伸出或缩回柱形腔内,并在导流体(303)的锥形尖端伸出时扎破一次性取样杯(5)底部,使经处理液处理过的粪便样本滴入到样本处理腔(101)下方设有的细菌培养机构(4)的培养皿(401)内进行细菌培养。3. A kind of pretreatment device for gastroenterology inspection for pathogenic bacteria in stool samples according to claim 1, characterized in that: the center of the cylinder (301) is provided with a cylindrical cavity with an open bottom end, in the center of the cylinder (301) A guide body (303) is slidably arranged in the cylindrical cavity, and a hydraulic drive cavity (302) is formed between the top of the guide body (303) and the top of the cylindrical cavity, and the hydraulic drive cavity (302) is connected to the first hydraulic input The tube (203) is communicated, so that the conical tip of the guiding fluid (303) is controlled by hydraulic oil to extend or retract into the cylindrical cavity, and the disposable sampling cup is punctured when the conical tip of the guiding fluid (303) extends (5) At the bottom, drop the fecal samples treated with the treatment liquid into the petri dish (401) of the bacterial culture mechanism (4) provided below the sample processing chamber (101) for bacterial culture. 4.根据权利要求3所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:所述导流体(303)内设置有引流通道(304),该引流通道(304)的底端开口在导流体(303)的底端面上,顶端连接有若干条倾斜向上延伸到其侧壁开口的引流孔(305),且这些引流孔(305)在导流体(303)伸出至极限位置时,与柱体(301)底端侧壁开设的进液孔(306)一一对应连通,从而使经处理的粪便样本液体经进液孔(306)、引流孔(305)和引流通道(304)滴入到培养皿(401)内。4. The pretreatment device for gastroenterology inspection for pathogenic bacteria in stool samples according to claim 3, characterized in that: a drainage channel (304) is provided in the guiding fluid (303), and the drainage channel ( The bottom end of 304) is opened on the bottom end surface of the guiding body (303), and the top end is connected with a plurality of drainage holes (305) extending obliquely upward to the opening of its side wall, and these drainage holes (305) are in the guiding body (303). When extending to the limit position, it is connected with the liquid inlet holes (306) opened on the side wall of the bottom end of the cylinder (301) in a one-to-one correspondence, so that the processed stool sample liquid can pass through the liquid inlet holes (306) and the drainage holes (305). ) and the drainage channel (304) are dropped into the petri dish (401). 5.根据权利要求4所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:所述进液孔(306)倾斜设置,其位置高的开口一端延伸到柱体(301)侧面的底端,位置低的一端连通柱形腔,进液孔(306)的内径从高处一端向低处一端逐渐缩小,在进液孔(306)内设置有过滤网,以过滤粪便样本液体中的杂质。5. The pretreatment device for gastroenterology inspection for pathogenic bacteria in fecal samples according to claim 4, characterized in that: the liquid inlet hole (306) is arranged at an inclination, and its high open end extends to the column The bottom end of the side of the body (301), the lower end is connected to the cylindrical cavity, the inner diameter of the liquid inlet (306) is gradually reduced from the high end to the lower end, and a filter screen is arranged in the liquid inlet (306), to filter impurities from the fecal sample fluid. 6.根据权利要求3所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:所述细菌培养机构(4)设置在壳体(1)的培养皿取放腔(108)内,且培养皿取放腔(108)的侧壁具有可打开的窗口,以通过该窗口取出或放入细菌培养机构(4)中预装有培养液的培养皿(401);培养皿(401)的两侧具有沿培养皿(401)取出或推入方向的轨道(402),在培养皿(401)的下方设置有保温腔(403),用于保持培养皿(401)内的温度。6. A pretreatment device for gastroenterology inspection of pathogenic bacteria in stool samples according to claim 3, characterized in that: the bacterial culture mechanism (4) is arranged on the petri dish of the housing (1) to pick and place Inside the cavity (108), and the side wall of the petri dish pick-and-place cavity (108) has an openable window, through which the petri dish (401) pre-filled with the culture liquid can be taken out or put into the bacterial culture mechanism (4) The two sides of the culture dish (401) are provided with tracks (402) along the direction of taking out or pushing the culture dish (401), and a heat preservation cavity (403) is provided below the culture dish (401) for keeping the culture dish (401). ) temperature. 7.根据权利要求1所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:环绕所述柱体(301)设置有若干个可翻转切割叶片(308),可翻转切割叶片(308)的顶端铰接在柱体(301)侧壁,且中部与柱体(301)之间具有柱状伸缩囊袋(309),该柱状伸缩囊袋(309)通过位于柱体(301)内的液压孔道与第二液压输入管(205)连通,通过向柱状伸缩囊袋(309)内注入或抽出液压油,使可翻转切割叶片(308)与柱体(301)保持50-80°夹角或贴在柱体(301)侧壁;7. A pretreatment device for gastroenterology inspection for pathogenic bacteria in stool samples according to claim 1, characterized in that: a plurality of reversible cutting blades (308) are arranged around the cylinder (301), The top end of the reversible cutting blade (308) is hinged on the side wall of the cylinder (301), and there is a cylindrical expansion bag (309) between the middle part and the cylinder (301). The hydraulic hole in (301) is communicated with the second hydraulic input pipe (205), and by injecting or extracting hydraulic oil into the cylindrical telescopic bag (309), the reversible cutting blade (308) and the cylinder (301) are kept at 50 degrees. -80° angle or stick to the side wall of the cylinder (301); 所述柱体(301)由一驱动机构(2)带动其自转,且利用回转接头(202)实现与外接液体管路的连通,所述驱动机构(2)的主体为电机(201)。The cylinder (301) is driven by a driving mechanism (2) to rotate, and uses a rotary joint (202) to communicate with an external liquid pipeline. The main body of the driving mechanism (2) is a motor (201). 8.根据权利要求7所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:所述样本处理探头(3)的顶端与驱动机构(2)连接,且驱动机构(2)设置在一块升降板(102)上,升降板(102)的边缘滑动设置在壳体(1)侧壁的竖直滑槽内,且升降板(102)将样本处理腔(101)的上部分隔出一个高度可变的设备腔(103),所述驱动机构(2)由设备腔(103)内的四组同步液压升降油缸(206)带动其升降,进而实现样本处理探头(3)的升降。8. A pretreatment device for gastroenterology inspection of pathogenic bacteria in stool samples according to claim 7, characterized in that: the top of the sample processing probe (3) is connected to the driving mechanism (2), and drives the The mechanism (2) is arranged on a lift plate (102), the edge of the lift plate (102) is slidably arranged in the vertical chute on the side wall of the housing (1), and the lift plate (102) moves the sample processing chamber (101) ) is divided into a height-variable equipment cavity (103), and the drive mechanism (2) is driven up and down by four sets of synchronous hydraulic lifting cylinders (206) in the equipment cavity (103), thereby realizing the sample processing probe ( 3) The lift. 9.根据权利要求1所述的一种消化科检验粪便样本中致病菌用的预处理装置,其特征在于:所述一次性取样杯(5)具有圆台形的杯体(501),杯体(501)的顶部和底部分别设置有薄膜封盖(502)和密封薄膜(503),杯体(501)顶部具有水平的外延部(504),该外延部(504)与样本处理腔(101)的固定腔(105)顶端配合固定。9. The pretreatment device for gastroenterology inspection for pathogenic bacteria in stool samples according to claim 1, characterized in that: the disposable sampling cup (5) has a truncated cup body (501), and the cup The top and bottom of the body (501) are respectively provided with a film cover (502) and a sealing film (503), and the top of the cup body (501) has a horizontal extension (504) which is connected to the sample processing chamber (504). The top of the fixing cavity (105) of 101) is fitted and fixed.
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