CN113142195A - Internal absorption antifreeze agent for fresh cut flowers, preparation method and use method thereof - Google Patents
Internal absorption antifreeze agent for fresh cut flowers, preparation method and use method thereof Download PDFInfo
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- CN113142195A CN113142195A CN202110369567.6A CN202110369567A CN113142195A CN 113142195 A CN113142195 A CN 113142195A CN 202110369567 A CN202110369567 A CN 202110369567A CN 113142195 A CN113142195 A CN 113142195A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
- A01N3/02—Keeping cut flowers fresh chemically
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- General Health & Medical Sciences (AREA)
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- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a systemic antifreeze agent for fresh cut flowers, which is prepared from S-abscisic acid, ethanol, citric acid, tween-20 and water, and the preparation method of the systemic antifreeze agent for fresh cut flowers comprises the following steps: weighing the raw materials according to the proportion; mixing the weighed S-abscisic acid and ethanol to prepare the bacteriostatic agent; adding the weighed water into a measuring cup, and then adding the weighed citric acid to obtain a first mixed solution; adding the weighed tween-20 into the first mixed solution to obtain a second mixed solution; and adding the prepared bacteriostatic agent into the second mixed solution to obtain the systemic antifreeze agent for the fresh cut flowers. A use method of a systemic antifreeze agent in fresh cut flowers comprises the following steps: preparing an antifreeze agent in the fresh cut flowers by using a maintenance bucket; putting the cut flowers into a maintenance bucket to absorb water; fishing out the cut flowers, controlling water, and placing in a refrigeration house; and then taking out the flowers from the refrigeration house to prepare the bouquet for shipment. The antifreeze agent in the fresh cut flowers can effectively promote the antifreeze capacity of the cut flowers at low temperature, reduce the probability of freezing injury of the cut flowers and promote the recovery of the cut flowers after the freezing injury.
Description
Technical Field
The invention relates to the field of gardening, in particular to a systemic antifreeze agent for fresh cut flowers, a preparation method and a use method thereof.
Background
The cut flower is cut from living plant, has ornamental value, and can be used for making plant materials such as stem, leaf, flower, fruit, etc. for flower decoration such as flower basket, bouquet, wreath, vase flower, wall flower, etc. The cut flowers are divided into cut flowers, cut leaves and cut branches, are suitable for manufacturing flower baskets, flower bundles, vase flowers and the like, and have ornamental value. The subdivision can be divided into cutting flower, cutting leaf, cutting branch, cutting fruit, etc.
When the atmospheric temperature is reduced to below zero ℃, some plants are frozen and killed due to the fact that the plants cannot resist severe cold, and other plants can stand upright in the ice and snow and are full of life. This is due to the significant differences in frost tolerance of different plants. The biological membrane system in the plant organism cell has close relation with the frost resistance of the plant; when plants are exposed to low temperature, the biological membrane is subjected to phase transition, namely, the biological membrane is changed into a gel state from a liquid crystal phase, the structure of the biological membrane is destroyed, and consequently, a non-lipid 'cave' with high permeability is formed on the plasma membrane and becomes a channel for freely inputting and outputting a plurality of electrolytes, and the sol in the cytoplasm is discharged in a large amount, and finally the plants are killed. Therefore, the frost-resistant plants must be able to maintain the normal liquid crystal phase of the biological membrane in the organism when the temperature is reduced; secondly, the stability on the membrane structure is provided, and the stability is positively correlated with the cold resistance of plant varieties; thirdly, the icing in the cells can be avoided, so that the damage of ice crystals to the membrane can be prevented; fourthly, the water in the cells flowing outside the cells freezes to cause certain damage, because the freeze dehydration causes the drought of the cells, denatures the protein, and also causes the contraction and collapse of the cells, so that the plasma membrane of the cells is damaged. Therefore, the performance of freeze and dehydration resistance is also required.
The cut flowers are easy to be frozen in the weather environment below 0 ℃ because no heat preservation measures are taken in the transportation process, and the cut flowers are not easy to recover after being frozen. Especially, the flower material of fleshy stem has higher probability of frostbite because of higher water content. Therefore, it is necessary to provide a fresh cut flower internal freezing resistant agent which can effectively promote the freezing resistance of the cut flower at low temperature, reduce the freezing injury probability of the cut flower and promote the restoration of the cut flower after the freezing injury.
Disclosure of Invention
The invention aims to provide a systemic antifreeze agent for fresh cut flowers, a preparation method and a use method thereof, which can effectively promote the antifreeze capacity of the cut flowers at low temperature, reduce the probability of freezing injury of the cut flowers and promote the recovery of the cut flowers after the freezing injury.
In order to achieve the purpose, the internal absorption antifreeze agent for the fresh cut flowers is prepared from the following raw materials in parts by mass:
0.05-0.1 part of S-abscisic acid;
1-2 parts of ethanol;
2-3.7 parts of citric acid;
200.4-0.6 parts of tween-200;
5000 parts of water.
The invention also provides a preparation method of the systemic antifreeze agent for the fresh cut flowers, which comprises the following steps: the method comprises the following steps:
weighing the raw materials according to the proportion for later use;
mixing the weighed S-abscisic acid and the ethanol to prepare the bacteriostatic agent;
adding the weighed water into a measuring cup, then adding the weighed citric acid, and fully stirring and dissolving to obtain a first mixed solution;
adding the weighed tween-20 into the first mixed solution, and uniformly mixing to obtain a second mixed solution;
and adding the prepared bacteriostatic agent into the second mixed solution, and uniformly mixing to obtain the systemic antifreeze agent for the fresh cut flowers.
Wherein, in the step of adding the weighed water into a measuring cup, then adding the weighed citric acid, fully stirring and dissolving to obtain a first mixed solution:
the judgment standard of fully stirring and dissolving is as follows: the particles of citric acid could not be observed with the naked eye.
Wherein, in the step of adding the weighed water to the measuring cup:
the measuring cup has a capacity of 5L.
The invention also provides a use method of the systemic antifreeze agent for the fresh cut flowers, which comprises the following steps:
preparing an antifreeze agent in the fresh cut flowers by using a maintenance bucket;
putting the cut flowers into a maintenance bucket to absorb water;
fishing out the cut flowers, controlling water, and placing in a cold storage at 2-5 ℃;
and then taking out the flowers from the refrigeration house to prepare the bouquet for shipment.
Wherein, in the step of putting the cut flowers into a maintenance bucket for absorbing water:
the water absorption time is more than 4 hours.
Wherein, in the step of fishing out the cut flowers to control water and placing the cut flowers in a cold storage at 2-5 ℃:
the standing time is more than 30 hours.
The invention has the beneficial effects that: weighing the raw materials according to the proportion for later use; mixing the weighed S-abscisic acid and the ethanol to prepare the bacteriostatic agent; adding the weighed water into a measuring cup, then adding the weighed citric acid, and fully stirring and dissolving to obtain a first mixed solution; adding the weighed tween-20 into the first mixed solution, and uniformly mixing to obtain a second mixed solution; the prepared bacteriostatic agent is added into the second mixed solution and uniformly mixed to obtain the internal freezing resistant agent for the fresh cut flowers. The anti-freezing agent for the fresh cut flowers has wide application range, can be generally applied to most of the cut flowers, and is efficient, economical, low-toxicity and pollution-free.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of the steps of example 1 of the method for preparing the systemic antifreeze agent in fresh cut flowers according to the present invention.
FIG. 2 is a flow chart of the steps of example 2 of the method for preparing the systemic antifreeze agent in the fresh cut flowers according to the present invention.
FIG. 3 is a flow chart of the steps of example 3 of the method for preparing the systemic antifreeze agent in the fresh cut flowers according to the present invention.
FIG. 4 is a flow chart of the steps of the method for using the systemic anti-freezing agent in the fresh cut flowers according to the invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Embodiment 1, please refer to fig. 1, and the invention provides a systemic antifreeze agent for fresh cut flowers, which is prepared from the following raw materials in parts by mass:
0.075 part of S-abscisic acid; 1.5 parts of ethanol; 3.125 parts of citric acid; tween-200.5 parts; 5000 parts of water.
A preparation method of a systemic antifreeze agent for fresh cut flowers comprises the following steps:
s1: weighing the raw materials according to the proportion for later use;
s2: mixing 0.075 part of the S-abscisic acid and 1.5 parts of ethanol to prepare a bacteriostatic agent;
s3: adding 5000 parts of the weighed water into a measuring cup of 5L, then adding 3.125 parts of the weighed citric acid, and fully stirring and dissolving to obtain a first mixed solution;
s4: adding 0.5 part of the weighed tween-20 into the first mixed solution, and uniformly mixing to obtain a second mixed solution;
s5: and adding the prepared bacteriostatic agent into the second mixed solution, and uniformly mixing to obtain the systemic antifreeze agent for the fresh cut flowers.
In this embodiment, the step of mixing the weighed S-abscisic acid and ethanol to prepare the bacteriostatic agent comprises: weighing the S-abscisic acid and the ethanol according to the proportion for later use; mixing the weighed S-abscisic acid and the ethanol and dissolving; standing after the dissolution is finished to obtain the bacteriostatic agent. The step of mixing and dissolving the weighed S-abscisic acid and the weighed ethanol comprises the following specific steps: the S-abscisic acid is first added into a test tube, then ethanol is added, and the mixture is fully shaken and dissolved. Standing after the dissolution is completed to obtain the bacteriostatic agent, wherein the step of standing comprises the following steps: the standing time was 2 hours. Adding the weighed water into a measuring cup, then adding the weighed citric acid, fully stirring and dissolving to obtain a first mixed solution: the judgment standard of fully stirring and dissolving is as follows: the particles of citric acid could not be observed with the naked eye.
Embodiment 2, please refer to fig. 2, which provides a systemic antifreeze agent for fresh cut flowers, which is prepared from the following raw materials in parts by mass:
0.1 part of S-abscisic acid; 2 parts of ethanol; 3.125 parts of citric acid; tween-200.5 parts; 5000 parts of water.
A preparation method of a systemic antifreeze agent for fresh cut flowers comprises the following steps:
s1: weighing the raw materials according to the proportion for later use;
s2: mixing 0.1 part of the weighed S-abscisic acid with 2 parts of ethanol to prepare a bacteriostatic agent;
s3: adding 5000 parts of the weighed water into a measuring cup of 5L, then adding 3.125 parts of the weighed citric acid, and fully stirring and dissolving to obtain a first mixed solution;
s4: adding 0.5 part of the weighed tween-20 into the first mixed solution, and uniformly mixing to obtain a second mixed solution;
s5: and adding the prepared bacteriostatic agent into the second mixed solution, and uniformly mixing to obtain the systemic antifreeze agent for the fresh cut flowers.
In this embodiment, the step of mixing the weighed S-abscisic acid and ethanol to prepare the bacteriostatic agent comprises: weighing the S-abscisic acid and the ethanol according to the proportion for later use; mixing the weighed S-abscisic acid and the ethanol and dissolving; standing after the dissolution is finished to obtain the bacteriostatic agent. The step of mixing and dissolving the weighed S-abscisic acid and the weighed ethanol comprises the following specific steps: the S-abscisic acid is first added into a test tube, then ethanol is added, and the mixture is fully shaken and dissolved. Standing after the dissolution is completed to obtain the bacteriostatic agent, wherein the step of standing comprises the following steps: the standing time was 2 hours. Adding the weighed water into a measuring cup, then adding the weighed citric acid, fully stirring and dissolving to obtain a first mixed solution: the judgment standard of fully stirring and dissolving is as follows: the particles of citric acid could not be observed with the naked eye.
Embodiment 3, please refer to fig. 3, which provides a systemic antifreeze agent for fresh cut flowers, comprising the following raw materials in parts by mass:
0.05 part of S-abscisic acid; 1 part of ethanol; 3.125 parts of citric acid; tween-200.5 parts; 5000 parts of water.
A preparation method of a systemic antifreeze agent for fresh cut flowers comprises the following steps:
s1: weighing the raw materials according to the proportion for later use;
s2: mixing 0.05 part of the weighed S-abscisic acid with 1 part of the ethanol to prepare the bacteriostatic agent;
s3: adding 5000 parts of the weighed water into a measuring cup of 5L, then adding 3.125 parts of the weighed citric acid, and fully stirring and dissolving to obtain a first mixed solution;
s4: adding 0.5 part of the weighed tween-20 into the first mixed solution, and uniformly mixing to obtain a second mixed solution;
s5: and adding the prepared bacteriostatic agent into the second mixed solution, and uniformly mixing to obtain the systemic antifreeze agent for the fresh cut flowers.
In this embodiment, the step of mixing the weighed S-abscisic acid and ethanol to prepare the bacteriostatic agent comprises: weighing the S-abscisic acid and the ethanol according to the proportion for later use; mixing the weighed S-abscisic acid and the ethanol and dissolving; standing after the dissolution is finished to obtain the bacteriostatic agent. The step of mixing and dissolving the weighed S-abscisic acid and the weighed ethanol comprises the following specific steps: the S-abscisic acid is first added into a test tube, then ethanol is added, and the mixture is fully shaken and dissolved. Standing after the dissolution is completed to obtain the bacteriostatic agent, wherein the step of standing comprises the following steps: the standing time was 2 hours. Adding the weighed water into a measuring cup, then adding the weighed citric acid, fully stirring and dissolving to obtain a first mixed solution: the judgment standard of fully stirring and dissolving is as follows: the particles of citric acid could not be observed with the naked eye.
By combining examples 1 to 3, it can be seen that:
the fresh cut flower systemic antifreeze agent provided by the invention is an efficient, economical, low-toxicity and pollution-free fresh cut flower systemic antifreeze agent and a method comprehensively developed from the aspects of enhancing antifreeze and the like. The systemic antifreeze agent for the fresh cut flowers is widely suitable for various flowers, and is particularly suitable for flowers such as six flowers, orchids, lilies, paeonia suffruticosa and the like. The S-abscisic acid can be quickly absorbed into the cells of the cut flowers by using the internal freezing resistant agent of the fresh cut flowers, so that the generation of freezing resistant factors is induced, and the freezing injury degree and the recovery capability of the cut flowers are effectively reduced. Taking cymbidium sinense and sixposed flowers as examples, after the cymbidium sinense and sixposed flowers absorb the antifreeze agent, the frostbite degree of the cymbidium sinense and the sixposed flowers can be effectively reduced when the environment with the low temperature below 0 ℃ is dealt with.
The citric acid is used as a pH value regulator, and when the pH value is lower, the flow of water can be accelerated, the water absorption capacity of the flower stems is enhanced, and anti-freezing components can quickly reach the cut flower cells; the S-abscisic acid is a plant growth balance factor, is a natural abscisic acid with an original name, is a pure natural product contained in all green plants, and has the functions of cultivating sources, consolidating the foundation and enhancing the comprehensive resistance (drought resistance, heat resistance, cold resistance, disease and insect resistance, salt and alkali resistance and the like) of the plants. Has important effects on drought resistance, water conservation, disaster reduction, yield conservation and ecological environment restoration in agricultural production. (ii) a Tween-20 can activate the surface of the catheter and prevent foreign matters from blocking the flower trunk and branch pipelines.
In addition, the preparation method of the systemic antifreeze agent for the fresh cut flowers is simple, and the systemic antifreeze agent can be prepared by only uniformly mixing various raw materials. The internal-absorption antifreeze agent for the fresh cut flowers needs to be subjected to internal absorption, induces the generation of cold-resistant factors and the like, and if the internal absorption is completed, the antifreeze agent is delivered immediately or placed in a frostbite environment, the expected effect cannot be achieved.
Therefore, the internal-absorption anti-freezing agent for the fresh cut flowers can effectively promote the freezing resistance of the cut flowers at low temperature, reduce the probability of freezing injury of the cut flowers and promote the recovery of the cut flowers after the freezing injury. The anti-freezing agent for the fresh cut flowers has wide application range, can be generally applied to most of the cut flowers, and is efficient, economical, low-toxicity and pollution-free.
Referring to fig. 4, the present invention further provides a method for using the anti-freezing agent in the fresh cut flower, comprising the following steps:
s1: preparing an antifreeze agent in the fresh cut flowers by using a maintenance bucket;
s2: putting the cut flowers into a maintenance bucket to absorb water for more than 4 hours;
s3: fishing out the cut flowers, controlling water, and placing the cut flowers in a refrigeration house at the temperature of 2-5 ℃ for more than 30 hours;
s4: and then taking out the flowers from the refrigeration house to prepare the bouquet for shipment.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. A systemic antifreeze agent for fresh cut flowers is characterized by being prepared from the following raw materials in parts by mass:
0.05-0.1 part of S-abscisic acid;
1-2 parts of ethanol;
2-3.7 parts of citric acid;
200.4-0.6 parts of tween-200;
5000 parts of water.
2. The preparation method of the systemic antifreeze agent in the fresh cut flowers as claimed in claim 1, characterized by comprising the following steps:
weighing the raw materials according to the proportion for later use;
mixing the weighed S-abscisic acid and the ethanol to prepare the bacteriostatic agent;
adding the weighed water into a measuring cup, then adding the weighed citric acid, and fully stirring and dissolving to obtain a first mixed solution;
adding the weighed tween-20 into the first mixed solution, and uniformly mixing to obtain a second mixed solution;
and adding the prepared bacteriostatic agent into the second mixed solution, and uniformly mixing to obtain the systemic antifreeze agent for the fresh cut flowers.
3. The method for preparing systemic antifreeze agent in fresh cut flowers as claimed in claim 2, wherein, in the step of adding the weighed water into a measuring cup, then adding the weighed citric acid, fully stirring and dissolving to obtain the first mixed solution:
the judgment standard of fully stirring and dissolving is as follows: the particles of citric acid could not be observed with the naked eye.
4. The method for preparing systemic antifreeze agent in fresh cut flowers as claimed in claim 3, wherein, in the step of adding the weighed water to the measuring cup:
the measuring cup has a capacity of 5L.
5. The use method of the systemic antifreeze agent in the fresh cut flowers as claimed in claim 4, characterized by comprising the following steps:
preparing an antifreeze agent in the fresh cut flowers by using a maintenance bucket;
putting the cut flowers into a maintenance bucket to absorb water;
fishing out the cut flowers, controlling water, and placing in a cold storage at 2-5 ℃;
and then taking out the flowers from the refrigeration house to prepare the bouquet for shipment.
6. The method for using the systemic antifreeze agent in the fresh cut flowers as claimed in claim 5, characterized in that, in the step of putting the cut flowers into a curing bucket for absorbing water:
the water absorption time is more than 4 hours.
7. The use method of the systemic antifreeze agent in the cut flowers as claimed in claim 6, characterized in that in the step of fishing out the cut flowers for controlling water and placing the cut flowers in a refrigerator at 2-5 ℃:
the standing time is more than 30 hours.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05186303A (en) * | 1991-02-04 | 1993-07-27 | Sankyo Co Ltd | Flowering promoter |
CN110742067A (en) * | 2019-11-18 | 2020-02-04 | 常州春海园艺有限公司 | Anti-freezing solution for flower seedlings |
-
2021
- 2021-04-07 CN CN202110369567.6A patent/CN113142195A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05186303A (en) * | 1991-02-04 | 1993-07-27 | Sankyo Co Ltd | Flowering promoter |
CN110742067A (en) * | 2019-11-18 | 2020-02-04 | 常州春海园艺有限公司 | Anti-freezing solution for flower seedlings |
Non-Patent Citations (9)
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