CN113106016A - A photosynthetic reactor fermentation tube for cultivating little algae - Google Patents
A photosynthetic reactor fermentation tube for cultivating little algae Download PDFInfo
- Publication number
- CN113106016A CN113106016A CN202110524043.XA CN202110524043A CN113106016A CN 113106016 A CN113106016 A CN 113106016A CN 202110524043 A CN202110524043 A CN 202110524043A CN 113106016 A CN113106016 A CN 113106016A
- Authority
- CN
- China
- Prior art keywords
- main body
- fermentation
- fermentation tank
- tank main
- gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 101
- 230000004151 fermentation Effects 0.000 title claims abstract description 101
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 22
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 20
- 238000012258 culturing Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 238000005273 aeration Methods 0.000 claims description 14
- 239000002184 metal Substances 0.000 claims description 10
- 229910001220 stainless steel Inorganic materials 0.000 claims description 9
- 239000010935 stainless steel Substances 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 6
- 238000007599 discharging Methods 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 44
- 239000000243 solution Substances 0.000 description 17
- 235000016425 Arthrospira platensis Nutrition 0.000 description 12
- 240000002900 Arthrospira platensis Species 0.000 description 12
- 229940082787 spirulina Drugs 0.000 description 12
- 238000000034 method Methods 0.000 description 7
- 235000013305 food Nutrition 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 108060006184 phycobiliprotein Proteins 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000001651 autotrophic effect Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 239000000049 pigment Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- SJWWTRQNNRNTPU-ABBNZJFMSA-N fucoxanthin Chemical compound C[C@@]1(O)C[C@@H](OC(=O)C)CC(C)(C)C1=C=C\C(C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)C(=O)C[C@]1(C(C[C@H](O)C2)(C)C)[C@]2(C)O1 SJWWTRQNNRNTPU-ABBNZJFMSA-N 0.000 description 2
- AQLRNQCFQNNMJA-UHFFFAOYSA-N fucoxanthin Natural products CC(=O)OC1CC(C)(C)C(=C=CC(=CC=CC(=CC=CC=C(/C)C=CC=C(/C)C(=O)CC23OC2(C)CC(O)CC3(C)C)C)CO)C(C)(O)C1 AQLRNQCFQNNMJA-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000009919 sequestration Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 1
- 229910003243 Na2SiO3·9H2O Inorganic materials 0.000 description 1
- 241001104939 Nitzschia laevis Species 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000003170 nutritional factors Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000021404 traditional food Nutrition 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a fermentation tube of a photosynthetic reactor for culturing microalgae, belonging to the technical field of microalgae culture. The photosynthetic reactor fermentation tube comprises a fermentation tank main body, wherein an LED lamp is arranged on the inner wall of the fermentation tank main body, a pH sensor, a temperature sensor and a gas concentration sensor are arranged in the fermentation tank main body, the top of the fermentation tank main body is provided with a gas inlet, a feed hole and a gas exhaust hole, the gas inlet is communicated with an air compressor and a gas cylinder, the gas exhaust hole is communicated with a gas exhaust port, and the feed hole is connected with a feed inlet; a liquid outlet is arranged on the side wall of the fermentation tank main body; the outside parcel of fermentation cylinder main part has temperature regulator. The invention can cultivate and screen excellent algae species of target products by controlling single factor variable and under the conditions of temperature, pH value, gas concentration, different nutrient substances and the like, and has the advantages of high fermentation efficiency, high discharging effect, simple structure and convenient use.
Description
Technical Field
The invention relates to a fermentation tube of a photosynthetic reactor for culturing microalgae, belonging to the technical field of microalgae culture.
Background
With the global environmental pollution aggravation, the climate continuous change, the population continuous growth and the resource exhaustion, how to ensure the safety, nutrition and sustainable food supply faces a huge challenge. These challenges place new demands on future food supply means and functions. The cell factory manufacturing is utilized to replace the traditional food obtaining mode, a sustainable new food manufacturing mode is established, the requirements of food production on resources and energy sources are greatly reduced, the emission of greenhouse gases is reduced, the controllability of food production and manufacturing is improved, and potential food safety risks and health risks are effectively avoided. Microalgae are one of the unutilized resources that are considered as promising and powerful cell factories due to their important role in global sustainable development initiatives such as sustainable industry, sustainable agriculture and ecological economy, and in particular as renewable resources for various natural metabolites (proteins, lipids, fuels, biochemical activities, pigments, etc.) are receiving great attention. Microalgae are considered to be more suitable for the production of various biochips than other types of plants and organic resources, and have the following characteristics: 1) microalgae can be planted and cultured on non-cultivated land, so that competition with agricultural land is avoided; 2) the life cycle of the microalgae is short, the cell structure and the tissue are simple, and the operation and the regulation and the control are convenient to carry out as required; 3) microalgae can be grown and cultured under different conditions for a full period of time, facilitating large-scale production to improve their harvesting capacity. Therefore, the cultivation and screening of high-yield and high-efficiency microalgae species and the screening of excellent microalgae species have extremely important significance in the aspects of environment and energy.
The cultivation of microalgae using fermenters is typically carried out using cell factory technology to obtain abundant resources. The existing fermentation tank main body has the disadvantages of slow fermentation efficiency in the use process, poor microalgae quality, low utilization rate and the like, and has a complex structure and high equipment cost.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a fermentation tube of a photosynthetic reactor for culturing microalgae, the main body of the fermentation tube can culture and screen excellent algae species of target products under the conditions of temperature, pH value, gas concentration, different nutrient substances and the like by controlling single factor variable, and the fermentation tube has the advantages of high fermentation efficiency, high discharging effect, simple structure, convenient use and low use cost.
The invention provides a fermentation tube of a photosynthetic reactor for culturing microalgae, which comprises a fermentation tank main body, wherein an LED lamp is arranged on the inner wall of the fermentation tank main body, a pH sensor, a temperature sensor and a gas concentration sensor are arranged in the fermentation tank main body, the top of the fermentation tank main body is provided with a gas inlet, a feed hole and an exhaust hole, the gas inlet is communicated with an air compressor and a gas cylinder, the exhaust hole is communicated with an exhaust port, and the feed hole is connected with a feed inlet; a liquid outlet is arranged on the side wall of the fermentation tank main body; the outer side of the fermentation tank main body is wrapped with a temperature regulator; the top of the fermentation tank main body is also fixedly provided with a rotating motor, the rotating motor is guided into the fermentation tank main body through a stainless steel metal pipe, and the stainless steel metal pipe is fixedly provided with four-blade type fan blades; the fermentation cylinder main part is connected with the main control machine, the main control machine communicates with LED lamp, pH value sensor, temperature sensor, gas concentration sensor, temperature regulation board and rotating electrical machines.
In one embodiment of the invention, a one-way valve is arranged between the air inlet and the air compressor, a one-way valve is arranged between the feed hole and the feed inlet, a one-way valve is arranged between the exhaust hole and the exhaust port, and a one-way valve is arranged between the liquid outlet and the fermentation tank main body.
In one embodiment of the present invention, the inner wall of the fermenter body is opaque, and the fermenter body has a shape of a cylinder, a cube or a sphere.
In one embodiment of the present invention, the air compressor is communicated with an air flow meter, and the gas cylinder is communicated with a gas flow meter.
In one embodiment of the present invention, the air flow meter and the gas flow meter are both communicated with a mixed gas flow meter, the mixed gas flow meter is communicated with the mixed gas flow meter, and the mixed gas flow meter is communicated with the gas inlet.
In one embodiment of the invention, the LED lamp emits white, red, yellow, green and blue light, the intensity of the LED lamp light is 1.0 Klx-10.0 Klx, and the frequency of the LED lamp light is 1 Hz-30 Hz.
In one embodiment of the present invention, the stainless steel metal pipe is provided with a plurality of aeration openings, and aeration heads are fixedly installed in the aeration openings.
In one embodiment of the invention, the bottom of the fermentation tank main body is provided with a supporting leg, one end of the supporting leg, which is far away from the fermentation tank main body, is fixedly provided with a supporting seat, and the lower surface of the supporting seat is provided with at least one anti-skid protrusion.
Advantageous effects
1. According to the fermentation tank main body, the aeration pipe, the aeration head and the air compressor are arranged, so that the culture solution in the fermentation tank main body can be aerated through the air compressor and the aeration pipe in the fermentation process of the fermentation tank main body, algae can be contacted with the required gas more thoroughly, and the fermentation efficiency is improved.
2. The fermentation tank main body controls the temperature in the fermentation tank by arranging the heating component, the temperature sensor and the main controller, so that microalgae can be thoroughly cultured at proper temperature.
3. According to the fermentation tank main body, the rotating motor and the rotating blade are arranged, so that microalgae in the fermentation tank main body can better contact with a culture solution and a light source to perform sufficient photosynthesis through the rotation of the rotating motor, meanwhile, the microalgae culture solution is driven to be rapidly discharged from the liquid outlet, the situation that the fermented raw material blocks the liquid outlet is avoided, and the discharging efficiency is improved.
4. The fermentation tank main body of the invention cultivates and screens excellent algae seeds of target products under optimal conditions by controlling single factor variable and under the conditions of temperature, pH value, gas concentration, different nutrient substances and the like.
Drawings
FIG. 1 is a schematic structural diagram of a fermentation tube of a photosynthetic reactor for culturing microalgae according to the present invention.
Wherein: 1. a fermenter main body; 2. an LED lamp; 3. a pH sensor; 4. a temperature sensor; 5. a gas concentration sensor; 6. a temperature adjusting plate; 7. an air inlet; 8. an exhaust port; 9. a feed inlet; 9-1, a feed hole; 10. a liquid outlet; 11. an air compressor; 12. a gas cylinder; 13. a main control machine; 13-1, operating interface; 18. a rotating electric machine; 18-1, four-bladed fan blades; 19. and (5) supporting legs.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments and the accompanying drawings. In which like parts are designated by like reference numerals. It should be noted that the words "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to directions in the drawings. The terms "inner" and "outer" are used to refer to directions toward and away from, respectively, the geometric center of a particular component.
Example 1
The utility model provides a photosynthetic reactor fermentation tube for cultivateing little algae, as shown in figure 1, including fermentation cylinder main part 1, LED lamp 2, pH value sensor 3, temperature sensor 4, gas concentration sensor 5, temperature regulation board 6, air compressor 11, gas cylinder 12 and main control machine 13, fermentation cylinder main part 1 is used for the splendid attire to carry out the little algae of photosynthesis, the inner wall of fermentation cylinder main part 1 is equipped with LED lamp 2, and little algae in the fermentation cylinder main part l can receive the light that LED lamp 2 sent. The inner wall of the fermentation tank body 1 is opaque, the fermentation tank body 1 is preferably cylindrical, and the size can be as follows: the height is 50cm, the diameter is 24cm, and the volume is 20L, but the fermentation tank can be a fermentation tank with other shapes as long as the technical effect required by the embodiment of the invention can be met. The LED lamp 2 has a complete waterproof function and can normally work in liquid all the time.
An air inlet 7 is formed in the top of the fermentation tank main body 1, the air inlet 7 is connected with a pipeline outwards, and the air inlet 7 is communicated with an air compressor 11 and an air bottle 12 through the pipeline. The air compressor 11 is used for inputting compressed air into the interior of the fermentation tank main body 1 for microalgae growth; the gas cylinder 12 is used for inputting the gas stored inside into the fermentation tank main body 1 for the growth of microalgae. The air compressor 11 is communicated with an air flow meter, the air bottle 12 is communicated with a gas flow meter, the air flow meter and the gas flow meter are both communicated with a mixed gas flow meter, the mixed gas flow meter is communicated with the air inlet 7, and the design is used for controlling the amount of the air and the gas which are input into the fermentation tank main body 1.
The air inlet 7 is connected with the intake pipe to 1 in the fermentation cylinder main part, the intake pipe can guarantee that the interior little algae of fermentation cylinder main part 1 can the abundant gas that contacts let in, the intake pipe can extend the bottom of fermentation cylinder main part 1, also can be other forms, as long as can reach the technological effect that this application will reach can.
The feeding hole 9-1 is connected with a feeding pipe into the fermentation tank main body 1, the feeding hole 9-1 is connected with a pipeline outside the fermentation tank main body 1, the other end of the pipeline is provided with a feeding hole 9, and microalgae seed liquid to be cultured and screened is inoculated into the fermentation tank main body 1 through the feeding hole 9 or other nutrient substances are put into the fermentation tank main body 1.
The fermentation tank main body 1 is further provided with an exhaust hole 8-1 and a liquid outlet 10, the exhaust hole 8-1 is connected with a pipeline, a valve and an exhaust port 8 outwards, gas is exhausted outwards through the exhaust port 8 under the control of the pipeline and the valve, and the size and the number of the exhaust holes 8-1 are not limited in the embodiment as long as the requirements of the embodiment of the application can be met. The flow rate of liquid outlet 10 is controlled through the valve that is connected with liquid outlet 10, does not do the restriction in the size of liquid outlet 10 and the form this embodiment yet, as long as can take out through liquid outlet 10 and cultivate the algae species of screening. In this embodiment, it is preferable that the air inlet 7, the air outlet 8 of the air exhaust system, the air outlet 8-1, the feed inlet 9 of the feed system, and the feed inlet 9-1 are all disposed on the top of the fermenter main body 1, and all have valve control switches.
A pH value sensor 3, a temperature sensor 4, a gas concentration sensor 5 and four-blade type fan blades 18-1 are arranged in the fermentation tank main body 1 to detect the pH value and the temperature in the fermentation tank main body 1. In order to effectively detect the pH value and the temperature of the culture solution and the algae species contained in the fermentation tank main body 1, preferably, the pH value sensor 3 and the temperature sensor 4 are extended into the culture solution of the culture solution and the algae species, the top of the fermentation tank main body is also fixedly provided with a rotating motor 18, the rotating motor 18 is guided into the fermentation tank main body 1 through a stainless steel metal pipe, the stainless steel metal pipe is fixed with a four-blade type fan blade 18-1, the stainless steel metal pipe is provided with a plurality of aeration ports, aeration heads are fixedly arranged in the aeration ports, and the aeration heads uniformly spray the gas into the fermentation tank.
The temperature adjusting plate 6 is wrapped on the outer side of the fermentation tank main body 1, and the temperature of the culture solution and the algae seeds contained in the fermentation tank main body 1 can be adjusted by adjusting the temperature adjusting plate 6, such as heating or condensing.
The LED lamp 2 is a device for providing a light source for the growth of the algae seeds in the fermentation tank main body 1, the LED lamp of the embodiment is preferably a novel LED controllable lamp, can be adjusted to various colors such as white, red, yellow, green and blue, can be adjusted to various intensities in the range of 1.0Klx to 10.0Klx, and can also be adjusted to various frequencies in the range of 1Hz to 30Hz, so that the novel LED controllable lamp can be fully and more suitable for the cultivation of the microalgae.
Fermentation cylinder main part 1 is connected with main control machine 13, main control machine 13 and LED lamp 2, pH value sensor 3, temperature sensor 4, gas concentration sensor 5, temperature regulation board 6 and the equal circular telegram of gas stirring favourable turn 18 are connected to put the electric wire in the creel stand, provide light source, pH value sensor 3 test pH value, gas concentration sensor 5 survey concentration, temperature sensor 4 measurement temperature, temperature regulation board 6 heating or condensation, stirring favourable turn 18 rotational speed with control LED lamp 2. The control device of the main controller 13 can also be electrically connected with the air flow meter, the gas flow meter, the mixed gas flow meter and the mixed gas flow meter, wires are arranged in the creel stand, and the control of the gas quantity introduced into the fermentation tank main body 1 is realized by controlling the air flow meter, the gas flow meter, the mixed gas flow meter and the mixed gas flow meter. An operation interface 13-1 is arranged on the main control machine 13, and the information of pH value, temperature and the like is displayed by opening and closing the operation interface 13-1.
The bottom of fermentation cylinder main part is equipped with supporting leg 19, the equal fixed mounting of one end that the fermentation cylinder body was kept away from to the supporting leg has the supporting seat, the lower surface of supporting seat all is provided with at least one non-slip raised.
On the basis of the above embodiment, in the embodiment of the present application, a plurality of nutrients required for cultivating microalgae can be added from the sample inlet 9 and the valve is controlled to be opened or closed.
The working principle of the invention is as follows: this fermentation cylinder is when using, at first add the culture solution toward fermentation cylinder body 1 in through feed inlet 9, later start light-emitting component 2, air compressor 11, temperature control board 6 and rotating electrical machines 18, at the in-process of cultivateing, can pass through air compressor 11, gas cylinder and aeration system aerate this internal culture solution of fermentation cylinder, the nutrient distribution who makes the culture solution is balanced, little algae fully contacts various cultivation conditions, the fermentation efficiency is improved, for this internal suitable temperature that provides of fermentation cylinder through hot plate and temperature control system, make the fermentation more thorough. On the other hand, the waste gas and the surplus gas generated in the cultivation process can be discharged through the exhaust port 8, and in the cultivation process, the rotating motor 18 is started at a proper rotating speed, so that the microalgae can better contact the culture solution and the light source to perform sufficient photosynthesis. When the microalgae are collected, the rotating motor 18 fully drives the culture solution and the microalgae to be discharged, so that the condition of blocking the liquid outlet 10 is avoided, and the discharge efficiency is improved.
The fermentation tube of the photosynthetic reactor for culturing the microalgae provided by the embodiment can be used for culturing and screening excellent algae species of target products of optimal conditions, including but not limited to functional substances such as proteins, pigments, fats, carbohydrates, amino acids and the like, under conditions such as temperature, pH value, gas concentration, illumination conditions, nutritional factors and the like by controlling single factor variables.
Example 2
In this embodiment, the photosynthetic reactor fermenter provided in embodiment 1 is used to culture Spirulina, which is a characteristic alga with a fast growth speed, strong stress tolerance, high content of functional substances, and the like, and is a commonly used alga in biological carbon sequestration research, and the Spirulina of this embodiment is selected from dun top Spirulina (Spirulina dunnii), taking dun top Spirulina as an example, the culture conditions are optimized, and the yield of phycobiliprotein is increased; the method for culturing the spirulina specifically comprises the following steps:
the method comprises the following steps: temperature optimization
Inoculating spirulina seed liquid into fermenter, and controlling initial cell density at l.2 × 106Controlling pH within about 8 in the range of/mL, introducing air, controlling DO value (dissolved oxygen) within the range of 30% -50%, setting temperature gradients of 20 ℃, 25 ℃, 30 ℃, 35 ℃ and 40 ℃ at intervals of 5 ℃, culturing for 9 days, and obtaining the optimum culture temperature by measuring parameters such as cell density, dry weight, phycobiliprotein content and the like of the spirulina.
Step two: pH optimization
The initial cell density is controlled at l.2X 10 by using a single factor test method and the same temperature is optimized6Controlling the temperature to be 30 ℃ in the/mL range, introducing air, controlling the DO value (dissolved oxygen) to be 30% -50%, controlling the initial culture pH values to be 6, 7, 8, 9 and 10, and detecting the change of the pH value after 9 days of culture by using a pH value sensor to obtain the initial pH value with the maximum phycobiliprotein content.
Step three: autotrophic-heterotypic culture
Inoculating spirulina seed solution into fermenter with initial cell density of l.2 × 106and/mL, using autotrophic culture as a control, adding glucose after 1 day of autotrophic culture, performing heterotypic culture for 3-5 days, and observing the change of parameters such as cell size, cell density, dry weight and phycobiliprotein content, wherein the phenomena such as cell volume increase, cell density increase and the like are found in the autotrophic-heterotypic culture of spirulina compared with the autotrophic spirulina.
Step four: carbon sequestration efficiency optimization
Inoculating spirulina seed solution into fermenter with initial cell density of l.2 × 106Controlling pH to be about 8, introducing air and carbon dioxide, setting the concentration of the introduced carbon dioxide to be 10%, 20%, 30%, 40%, 50% and 100% by taking the introduced air as a reference, controlling DO (dissolved oxygen) value to be 30% -50%, culturing for 9 days, calculating carbon fixation rate, cell density, dry weight and phycobiliprotein content indexes, and obtaining high-yield phycobiliproteinCarbon dioxide concentration of the protein.
Example 3
This example uses the photosynthetic reactor fermenter proposed in example 1 to cultivate rhombohedral algae, the rhombohedral algae (Nitzschia laevis) in this example were selected from UTEX 2047, usa, the university of texas, austin, texas, the culture medium used was LDM medium, the composition ratio was 1g tryptone, 892mL artificial seawater, 100mL Bristol solution, 6mL PIV metal solution, 1mL biotin stock solution (25.0 × 10)-5g/L) and 1mL vitamin B12 (15.0X 10)-5g/L), all fermentation media are added with 120mg/L Na2SiO3·9H2O、0.68g/L NaNO3And 5g/L glucose, and the method for culturing the rhombohedral algae specifically comprises the following steps:
s1. the medium is adjusted to pH 8.5 and autoclaved at 121 ℃ for 20 minutes. The cell suspension was centrifuged at 3000g for 5 minutes and the cell particles were washed twice with distilled water and then transferred to the fermentor body containing fresh LDM medium. The pre-sterilized glucose stock solution was added to the medium at a final concentration of 5 g/L. The cell culture was cultured at 23 ℃ with shaking at 150 rpm. The mixed nutrient cultures were illuminated continuously with LED lights while the heterotrophic cultures were kept in the dark.
S2, the initial illumination intensity is respectively 0, 10, 20, 40 and 60 mu mol m-2s-1The influence of the light intensity on the cell growth and the fucoxanthin content was evaluated in the fermenter of (1). Then, white light, blue light and a mixture of the two (ratio 1: 1) were used to evaluate their effect on cell growth and pigment biosynthesis. Cells were heterotrophic for 24 hours by stimulation with mixed light in a photobioreactor fermentor.
By changing the condition of single factor, the optimal illumination condition with high fucoxanthin content and the culture condition of algae cells are found.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents may be substituted for elements thereof without departing from the scope of the invention.
Claims (8)
1. A fermentation tube of a photosynthetic reactor for culturing microalgae is characterized by comprising a fermentation tank main body, wherein an LED lamp is arranged on the inner wall of the fermentation tank main body, a pH sensor, a temperature sensor and a gas concentration sensor are arranged in the fermentation tank main body, the top of the fermentation tank main body is provided with a gas inlet, a feed hole and a vent hole, the gas inlet is communicated with an air compressor and a gas cylinder, the vent hole is communicated with a gas outlet, and the feed hole is connected with a feed inlet; a liquid outlet is arranged on the side wall of the fermentation tank main body; the outer side of the fermentation tank main body is wrapped with a temperature regulator; the top of the fermentation tank main body is also fixedly provided with a rotating motor, the rotating motor is guided into the fermentation tank main body through a stainless steel metal pipe, and the stainless steel metal pipe is fixedly provided with four-blade type fan blades; the fermentation cylinder main part is connected with the main control machine, the main control machine communicates with LED lamp, pH value sensor, temperature sensor, gas concentration sensor, temperature regulation board and rotating electrical machines.
2. A fermentation tube of a photosynthetic reactor for culturing microalgae according to claim 1 wherein a check valve is disposed between the air inlet and the air compressor, a check valve is disposed between the feed inlet and the feed inlet, a check valve is disposed between the exhaust outlet and the exhaust outlet, and a check valve is disposed between the liquid outlet and the fermentation tank body.
3. A photosynthetic reactor fermenter according to claim 2, wherein the inner wall of the fermenter body is opaque, and the fermenter body has a shape of a cylinder, a cube or a sphere.
4. A photosynthetic reactor fermentation tube for culturing microalgae according to claim 3 wherein the air compressor is communicated with an air flow meter and the gas cylinder is communicated with a gas flow meter.
5. A photosynthetic reactor fermentation tube for culturing microalgae according to claim 4 wherein the air flow meter and the gas flow meter are both in communication with a mixed gas flow meter, the mixed gas flow meter is in communication with a mixed gas flow meter, and the mixed gas flow meter is in communication with the gas inlet.
6. A photosynthetic reactor fermentation tube for culturing microalgae according to claim 5 wherein the LED lights emit white, red, yellow, green and blue light, the intensity of the LED light is 1.0 Klx-10.0 Klx, and the frequency of the LED light is 1 Hz-30 Hz.
7. A fermentation tube of a photosynthetic reactor for culturing micro algae according to claim 6, wherein the stainless steel metal tube is provided with a plurality of aeration ports, and aeration heads are fixedly arranged in the aeration ports.
8. A photosynthetic reactor fermentation tube for culturing microalgae according to claim 7 wherein the bottom of the fermenter main body is provided with support legs, one ends of the support legs, which are far away from the fermenter main body, are fixedly provided with support bases, and the lower surfaces of the support bases are provided with at least one anti-slip bulge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110524043.XA CN113106016A (en) | 2021-05-13 | 2021-05-13 | A photosynthetic reactor fermentation tube for cultivating little algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110524043.XA CN113106016A (en) | 2021-05-13 | 2021-05-13 | A photosynthetic reactor fermentation tube for cultivating little algae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113106016A true CN113106016A (en) | 2021-07-13 |
Family
ID=76722251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110524043.XA Withdrawn CN113106016A (en) | 2021-05-13 | 2021-05-13 | A photosynthetic reactor fermentation tube for cultivating little algae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113106016A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116399804A (en) * | 2023-03-15 | 2023-07-07 | 上海泽泉科技股份有限公司 | A photosynthetic measuring device for measuring liquid samples |
| IT202300023034A1 (en) * | 2023-11-02 | 2025-05-02 | Algae Spa | REACTOR FOR GROWING MICROALGAE |
-
2021
- 2021-05-13 CN CN202110524043.XA patent/CN113106016A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116399804A (en) * | 2023-03-15 | 2023-07-07 | 上海泽泉科技股份有限公司 | A photosynthetic measuring device for measuring liquid samples |
| IT202300023034A1 (en) * | 2023-11-02 | 2025-05-02 | Algae Spa | REACTOR FOR GROWING MICROALGAE |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105316217B (en) | Artificial light source both culturing microalgae equipment | |
| CN105316235A (en) | Freshwater eukaryoticmicroalgae culture method | |
| CN102827766B (en) | Photobioreactor for large-scale production of astaxanthin by haematococcus pluvialis | |
| CN110184193B (en) | A continuous gradient feeding method and device for efficient propagation of Haematococcus pluvialis | |
| CN113106016A (en) | A photosynthetic reactor fermentation tube for cultivating little algae | |
| CN103103128A (en) | Method for high efficiency enrichment culture of microalgae | |
| CN116574613A (en) | Chlorella pyrenoidosa chlorophyll synthesis deficient mutant strain and application thereof | |
| CN1314795C (en) | An airlift photobioreactor for high-density cultivation of Haematococcus pluvialis | |
| CN117229982B (en) | Application of N- (1, 3-dimethylbutyl) -N' -phenyl p-phenylenediamine-quinone in culture of synechocystis | |
| CN114907982A (en) | Chlorella mutant strain and high-density heterotrophic culture method and application | |
| CN102321537A (en) | Bubbling bioreactor used for plant tissue culture | |
| Tsygankov | Laboratory scale photobioreactors | |
| KR101287384B1 (en) | Method for increasing growht and lipid content of microalgae using mixture of led light | |
| CN110205250A (en) | One plant of cellulase high-yield and its screening technique and application | |
| CN105695356B (en) | Method for increasing the yield of Chlorella by two rounds of co-cultivation and method for preparing biological feed | |
| CN204097489U (en) | A kind of built-in light source bio-reactor and production cultivation equipment | |
| CN107384801A (en) | Closing biological membrane type cultural method for industrialized production microalgae | |
| CN203715619U (en) | Cylindrical air-lift type efficient photobioreactor for microalgae cultivation | |
| CN203683528U (en) | High-density continuous culture device of microalgae | |
| US12012581B2 (en) | Method and system for heterotrophic and mixotrophic cultivation of microalgae | |
| Voina et al. | Equipment for Increasing the Phototrophic Microalgae Production at Lab Scale | |
| CN113136345A (en) | Method for heterotrophic-autotrophic continuous culture of photosynthetic microorganisms, culture system and application thereof | |
| CN104593224A (en) | Special photo-biological reactor system for driving microalgae cultivation by multi-stage potential energy differences | |
| CN2763279Y (en) | Gas-lifting type photo-bioreactor for rain-growth red spherical chlorella high density culture | |
| Tókos et al. | Automation control system of an equipment for ELF stimulated phototrophic microalgae production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210713 |
|
| WW01 | Invention patent application withdrawn after publication |