CN113092699A - Method and device for monitoring ecological environment of water area by using flagellates - Google Patents
Method and device for monitoring ecological environment of water area by using flagellates Download PDFInfo
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- CN113092699A CN113092699A CN202110301131.3A CN202110301131A CN113092699A CN 113092699 A CN113092699 A CN 113092699A CN 202110301131 A CN202110301131 A CN 202110301131A CN 113092699 A CN113092699 A CN 113092699A
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- water
- flagellates
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- mesh bag
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 85
- 238000012544 monitoring process Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000004744 fabric Substances 0.000 claims description 11
- 239000004677 Nylon Substances 0.000 claims description 8
- 239000006260 foam Substances 0.000 claims description 8
- 229920001778 nylon Polymers 0.000 claims description 8
- 229910001220 stainless steel Inorganic materials 0.000 claims description 8
- 239000010935 stainless steel Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 239000000834 fixative Substances 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 2
- 239000003344 environmental pollutant Substances 0.000 description 7
- 231100000719 pollutant Toxicity 0.000 description 7
- 229910001385 heavy metal Inorganic materials 0.000 description 6
- 238000009825 accumulation Methods 0.000 description 4
- 231100000613 environmental toxicology Toxicity 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000006353 environmental stress Effects 0.000 description 3
- 239000002759 woven fabric Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the field of monitoring of ecological environment pollution of a water area, in particular to a method and a device for monitoring the ecological environment of the water area by utilizing flagellates. The technical scheme realizes the application of actively utilizing the flagellates to monitor the ecological environment of the water area.
Description
Technical Field
The invention relates to the field of monitoring of ecological environment pollution of a water area, in particular to a method and a device for monitoring the ecological environment of the water area by using flagellates.
Background
In recent years, water eutrophication, heavy metal pollution and other water area ecological environment pollution events occur frequently. The flagellates are important groups of plankton, are sensitive to various pollutants in the water environment, can enrich certain pollutants in cells, and have application value in monitoring the ecological environmental pollution of water areas. The prior art can not accurately monitor, evaluate and early warn the response of the flagellates to organic pollution stress and can not provide comparable basic data for environmental toxicology, pollutant accumulation dynamics and water quality purification due to the fact that the flagellates can freely move and have contingency in temporal instantaneity and spatial distribution. It is therefore desirable to design a method that can actively use flagellates for monitoring.
Disclosure of Invention
In order to solve the above problems, the present invention provides a method for monitoring the ecological environment of a water area by using flagellates, comprising the steps of:
s1, manufacturing a device which is provided with a water drain valve and can not leak flagellate;
s2, culturing the pre-cultured flagellates at 10%4The cell density of Cells/L is inoculated into the device in S1 through a water discharge valve, the water discharge valve of the device is closed, the device is slowly immersed into the water body to be monitored so that a water sample enters the device, and the device is fixed at a position 0.1 to 0.5 meter away from the water surface;
s3, taking out the device after a preset time, hanging for 3-5 minutes, and placing a prepared sample bottle below a water drain valve when the water sample in the device naturally leaks to 10-50 mL;
s4, opening a water drain valve, and transferring all the residual water samples containing the flagellates in the device into a sample bottle;
s5, adding fixative to the sample bottle or rapidly returning to the laboratory under low temperature storage condition for detection and comparison with original pre-cultured flagellate.
And the device for monitoring the ecological environment of the water area by using the flagellates for the method at least comprises a mesh bag main body, wherein the mesh bag main body is conical, the mesh bag main body is made of cloth with the aperture of 10 mu m, the upper end of the mesh bag main body is provided with a circular ring for fixing the shape of the mesh bag main body, the lower end of the mesh bag main body is provided with a water drain valve, the upper part of the mesh bag main body is connected with a foam floater through a connecting rope, and the lower part of the mesh bag main body is connected with a stainless steel block through a connecting rope.
Moreover, the cloth for manufacturing the mesh bag main body is nylon woven cloth.
Moreover, the circular ring is a stainless steel circular ring with the diameter of 15-30 cm.
And the circular ring and the water drain valve are sewed on the mesh bag main body, and the upper part of the mesh bag main body is sealed without an opening.
Moreover, the connecting rope is provided with a buckle for adjusting the length of the connecting rope.
And the net bag main body is connected with the foam floater through more than 3 connecting ropes uniformly distributed above the net bag main body.
The beneficial effects of this technical scheme lie in: the flagellates cultured in a laboratory are limited in the mesh bag device and are placed in a water area to be detected, and organic pollutants, heavy metal ions and the like dissolved and suspended in the water body can freely enter the mesh bag and are absorbed by the flagellates. After a certain monitoring time, concentrating a water sample and taking out the sample, qualitatively and quantitatively researching the response of the flagellates to environmental stresses such as organic pollution, heavy metal pollution and the like by taking cells cultured in a laboratory as a contrast, and providing a basis for environmental toxicology, pollutant accumulation dynamics and water quality purification engineering, thereby realizing the active monitoring of the ecological environment of a water area by using the flagellates.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the apparatus of the present invention;
description of reference numerals 1: foam float, 2: connecting rope, 3: buckle, 4: nylon woven fabric with pore diameter of 10 μm, 5: stainless steel ring, 6: water drain valve, 7: drain valve switch, 8: and (4) a stainless steel block.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and examples, and the present invention is not limited to the examples.
A method for monitoring the ecological environment of a water area by using flagellates comprises the following steps:
s1, manufacturing a device which is provided with a water drain valve and can not leak flagellates, wherein the device is made of nylon woven fabric and can slowly and naturally leak water, and the water drain valve is used for quickly draining water in the device;
s2, culturing the pre-cultured flagellates at 10%4The cell density of Cells/L is inoculated into the device in S1 through a water discharge valve, the water discharge valve of the device is closed, the device is slowly immersed into the water body to be monitored so that a water sample enters the device, the length of the rope is adjusted through a buckle on a device connecting rope, and the device is fixed at a position 0.1 to 0.5 meter away from the water surface;
s3, taking out the device after a preset time, hanging for 3-5 minutes, and placing a prepared sample bottle below a water drain valve after water in the device naturally leaks to the residual 10-50 mL from the nylon woven fabric;
s4, opening a water drain valve, and transferring all the residual water samples containing the flagellates in the device into a sample bottle;
s5, adding fixative or storing at low temperature, and rapidly returning to laboratory for each test, and comparing with original pre-cultured flagellate. Therefore, the response of the flagellates to environmental stresses such as organic pollution, heavy metal pollution and the like can be qualitatively and quantitatively researched, and a basis is provided for environmental toxicology, pollutant accumulation dynamics and water quality purification engineering.
Wherein the fixing agent in S5 is glutaraldehyde or Lugol iodine solution.
A device for monitoring the ecological environment of a water area by using flagellates in the method at least comprises a mesh bag main body which is conical as shown in figure 1, the mesh bag main body is made of cloth with the aperture of 10 mu m, the upper end of the mesh bag main body is provided with a circular ring for fixing the shape of the mesh bag main body, the lower end of the mesh bag main body is provided with a water drain valve 6, the upper part of the mesh bag main body is connected with a foam floater through a connecting rope, and the lower part of the mesh bag main body is connected with a stainless steel block 8 through a connecting rope. The reason why the pore diameter of the main body of the mesh bag is 10 μm is that the cell diameter of the flagellates is larger than 10 μm, and the diameter of the organic pollution particles is smaller than 10 μm, so that the function of absorbing pollutants in the water body to be monitored while leaking the flagellates can be realized.
A drain valve switch 7 is connected to the drain valve.
Moreover, the cloth for manufacturing the mesh bag main body is nylon woven cloth 4.
Moreover, the circular ring is a stainless steel circular ring 5 with the diameter of 15-30 cm.
And the circular ring and the water drain valve are sewed on the mesh bag main body, and the upper part of the mesh bag main body is sealed without an opening.
Moreover, the connecting rope is provided with a buckle 3 for adjusting the length of the connecting rope so as to adjust the length of the connecting rope.
And the pocket main part is connected with foam float 1 through evenly distributed connect rope 2 more than 3 of top to let foam float evenly atress, do benefit to the balance of pocket main part.
When in use, the flagellate is firstly mixed with 104The cell density of the Cells/L is inoculated into the mesh bag through the water drain valve, the device is placed in a water sample monitoring state for a certain time, then the device is taken out and hung, raw water leaks out through meshes of the nylon fabric, and flagellate Cells with the diameter larger than that of the meshes of the nylon fabric are intercepted and remained in the mesh bag. And when the residual water is leaked out by 10 to 50mL, discharging the concentrated water sample containing the dinoflagellate cells to a sample bottle through a lower water discharge valve. Because the raw water is tens of liters, the water quantity is overlarge, the cell density of algae is too low, and the subsequent indoor detection experiment operation is not facilitated, the water sample is concentrated by hanging and leaking water.
The beneficial effects of this technical scheme lie in: the flagellates cultured in a laboratory are limited in the mesh bag device and are placed in a water area to be detected, and organic pollutants, heavy metal ions and the like dissolved and suspended in the water body can freely enter the mesh bag and are absorbed by the flagellates. After a certain monitoring time, concentrating a water sample and taking out the sample, qualitatively and quantitatively researching the response of the flagellates to environmental stresses such as organic pollution, heavy metal pollution and the like by taking cells cultured in a laboratory as a contrast, and providing a basis for environmental toxicology, pollutant accumulation dynamics and water quality purification engineering, thereby realizing the active monitoring of the ecological environment of a water area by using the flagellates.
Claims (7)
1. A method for monitoring the ecological environment of a water area by using flagellates is characterized by comprising the following steps:
s1, manufacturing a device which is provided with a water drain valve and can not leak flagellate;
s2, culturing the pre-cultured flagellates at 10%4The cell density of Cells/L is inoculated into the device in S1 through a water discharge valve, the water discharge valve of the device is closed, the device is slowly immersed into the water body to be monitored so that a water sample enters the device, and the device is fixed at a position 0.1 to 0.5 meter away from the water surface;
s3, taking out the device after a preset time, hanging for 3-5 minutes, and placing a prepared sample bottle below a water drain valve when the water sample in the device naturally leaks to 10-50 mL;
s4, opening a water drain valve, and transferring all the residual water samples containing the flagellates in the device into a sample bottle;
s5, adding fixative to the sample bottle or rapidly returning to the laboratory under low temperature storage condition for detection and comparison with original pre-cultured flagellate.
2. A device for monitoring the ecological environment of a body of water by using flagellates according to the method of claim 1, comprising at least a body of mesh bag, wherein: the mesh bag main body is conical, the mesh bag main body is made of cloth with the aperture of 10 microns, the upper end of the mesh bag main body is provided with a circular ring for fixing the shape of the mesh bag main body, the lower end of the mesh bag main body is provided with a water drain valve, the upper part of the mesh bag main body is connected with a foam floater through a connecting rope, and the lower part of the mesh bag main body is connected with a stainless steel block through a connecting rope.
3. A device for monitoring the ecological environment of a body of water by using flagellates as claimed in claim 2, wherein: the cloth for manufacturing the mesh bag main body is nylon woven cloth.
4. A device for monitoring the ecological environment of a body of water by using flagellates as claimed in claim 2, wherein: the circular ring is a stainless steel circular ring with the diameter of 15-30 cm.
5. A device for monitoring the ecological environment of a body of water by using flagellates as claimed in claim 2, wherein: the circular ring and the water drain valve are sewn on the mesh bag main body, and the upper part of the mesh bag main body is sealed without an opening.
6. A device for monitoring the ecological environment of a body of water by using flagellates as claimed in claim 2, wherein: the connecting rope is provided with a buckle for adjusting the length of the connecting rope.
7. A device for monitoring the ecological environment of a body of water by using flagellates as claimed in claim 2, wherein: the net bag main body is connected with the foam floater through more than 3 connecting ropes uniformly distributed above the net bag main body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110301131.3A CN113092699A (en) | 2021-03-22 | 2021-03-22 | Method and device for monitoring ecological environment of water area by using flagellates |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110301131.3A CN113092699A (en) | 2021-03-22 | 2021-03-22 | Method and device for monitoring ecological environment of water area by using flagellates |
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| Publication Number | Publication Date |
|---|---|
| CN113092699A true CN113092699A (en) | 2021-07-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110301131.3A Pending CN113092699A (en) | 2021-03-22 | 2021-03-22 | Method and device for monitoring ecological environment of water area by using flagellates |
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| Country | Link |
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| CN (1) | CN113092699A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203069397U (en) * | 2012-12-26 | 2013-07-17 | 中国水产科学研究院珠江水产研究所 | Quantitative collection fishing net for floating algae in rivers |
| CN203083840U (en) * | 2013-02-07 | 2013-07-24 | 陕西理工学院 | Sampling device for periphytic algae in still water |
| CN203128543U (en) * | 2013-01-24 | 2013-08-14 | 中国水产科学研究院珠江水产研究所 | Physiological ecology research device for alga in natural water |
| CN105092806A (en) * | 2015-07-03 | 2015-11-25 | 黎佛林 | Diatom biological water quality monitoring system |
| CN107036851A (en) * | 2017-04-26 | 2017-08-11 | 湖北工业大学 | Settle algae collection device and the method for determining the algae rate of settling |
| CN107593631A (en) * | 2017-08-29 | 2018-01-19 | 暨南大学 | A kind of planktonic organism and particle matter collection device and application suitable for Large River |
| CN108165457A (en) * | 2017-12-29 | 2018-06-15 | 北京师范大学 | Biological habitat insitu analog device in a kind of water body |
| CN208588727U (en) * | 2018-07-16 | 2019-03-08 | 三峡大学 | Experimental provision for field algal culture and water sampling |
-
2021
- 2021-03-22 CN CN202110301131.3A patent/CN113092699A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203069397U (en) * | 2012-12-26 | 2013-07-17 | 中国水产科学研究院珠江水产研究所 | Quantitative collection fishing net for floating algae in rivers |
| CN203128543U (en) * | 2013-01-24 | 2013-08-14 | 中国水产科学研究院珠江水产研究所 | Physiological ecology research device for alga in natural water |
| CN203083840U (en) * | 2013-02-07 | 2013-07-24 | 陕西理工学院 | Sampling device for periphytic algae in still water |
| CN105092806A (en) * | 2015-07-03 | 2015-11-25 | 黎佛林 | Diatom biological water quality monitoring system |
| CN107036851A (en) * | 2017-04-26 | 2017-08-11 | 湖北工业大学 | Settle algae collection device and the method for determining the algae rate of settling |
| CN107593631A (en) * | 2017-08-29 | 2018-01-19 | 暨南大学 | A kind of planktonic organism and particle matter collection device and application suitable for Large River |
| CN108165457A (en) * | 2017-12-29 | 2018-06-15 | 北京师范大学 | Biological habitat insitu analog device in a kind of water body |
| CN208588727U (en) * | 2018-07-16 | 2019-03-08 | 三峡大学 | Experimental provision for field algal culture and water sampling |
Non-Patent Citations (3)
| Title |
|---|
| 张其余: "秋冬季河藻类数量特征分析及水质评价", 《山东水利》 * |
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Application publication date: 20210709 |
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