CN112899216A - 一种人参皂苷含量高的人参细胞培养方法 - Google Patents
一种人参皂苷含量高的人参细胞培养方法 Download PDFInfo
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- CN112899216A CN112899216A CN202110120858.1A CN202110120858A CN112899216A CN 112899216 A CN112899216 A CN 112899216A CN 202110120858 A CN202110120858 A CN 202110120858A CN 112899216 A CN112899216 A CN 112899216A
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- ginseng
- ginsenoside
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- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 94
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 94
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 94
- 229930182494 ginsenoside Natural products 0.000 title claims abstract description 23
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 30
- RWXIFXNRCLMQCD-JBVRGBGGSA-N (20S)-ginsenoside Rg3 Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1CC[C@]2(C)[C@H]3C[C@@H](O)[C@H]4[C@@]([C@@]3(CC[C@H]2C1(C)C)C)(C)CC[C@@H]4[C@@](C)(O)CCC=C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RWXIFXNRCLMQCD-JBVRGBGGSA-N 0.000 claims description 15
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 14
- CKUVNOCSBYYHIS-UHFFFAOYSA-N (20R)-ginsenoside Rg3 Natural products CC(C)=CCCC(C)(O)C1CCC(C2(CCC3C4(C)C)C)(C)C1C(O)CC2C3(C)CCC4OC1OC(CO)C(O)C(O)C1O CKUVNOCSBYYHIS-UHFFFAOYSA-N 0.000 claims description 12
- CKUVNOCSBYYHIS-IRFFNABBSA-N (20S)-ginsenoside Rh2 Chemical compound O([C@H]1CC[C@]2(C)[C@H]3C[C@@H](O)[C@H]4[C@@]([C@@]3(CC[C@H]2C1(C)C)C)(C)CC[C@@H]4[C@@](C)(O)CCC=C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O CKUVNOCSBYYHIS-IRFFNABBSA-N 0.000 claims description 12
- CKUVNOCSBYYHIS-LGYUXIIVSA-N 20(R)-Ginsenoside Rh2 Natural products O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@H]1C(C)(C)[C@H]2[C@@](C)([C@H]3[C@](C)([C@@]4(C)[C@H]([C@H](O)C3)[C@@H]([C@](O)(CC/C=C(\C)/C)C)CC4)CC2)CC1 CKUVNOCSBYYHIS-LGYUXIIVSA-N 0.000 claims description 12
- XIRZPICFRDZXPF-UHFFFAOYSA-N Ginsenoside Rg3 Natural products CC(C)=CCCC(C)(O)C1CCC(C2(CC(O)C3C4(C)C)C)(C)C1C(O)CC2C3(C)CCC4OC1OC(CO)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O XIRZPICFRDZXPF-UHFFFAOYSA-N 0.000 claims description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
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- 238000005457 optimization Methods 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
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- 238000012136 culture method Methods 0.000 claims 9
- 240000004371 Panax ginseng Species 0.000 claims 2
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
Abstract
Description
Claims (10)
Priority Applications (2)
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CN202110120858.1A CN112899216A (zh) | 2021-01-28 | 2021-01-28 | 一种人参皂苷含量高的人参细胞培养方法 |
PCT/CN2022/074383 WO2022161442A1 (zh) | 2021-01-28 | 2022-01-27 | 一种人参皂苷含量高的人参细胞培养方法 |
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WO (1) | WO2022161442A1 (zh) |
Cited By (1)
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WO2022161442A1 (zh) * | 2021-01-28 | 2022-08-04 | 深圳先声科技发展有限公司 | 一种人参皂苷含量高的人参细胞培养方法 |
Families Citing this family (1)
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CN116300479B (zh) * | 2023-05-22 | 2023-08-22 | 山东卫康生物医药科技有限公司 | 基于干细胞培养法的人参皂甙生产装置的控制方法及系统 |
Citations (5)
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US20100233813A1 (en) * | 2007-09-21 | 2010-09-16 | Unhwa Corporation | Plant stem cell line derived from cambium of herbaceious plant with storage root and method for isolating the same |
CN102302420A (zh) * | 2011-07-29 | 2012-01-04 | 金凤燮 | 红参皂苷Rg2组和Rh1组、制备方法及在制备抗皮肤老化化妆品中的应用 |
WO2016028129A1 (ko) * | 2014-08-22 | 2016-02-25 | 주식회사 운화 | 희귀 진세노사이드를 고함유하는, 산삼 또는 인삼을 포함하는 인삼류 추출물, 인삼류 형성층 유래 식물줄기세포 또는 이의 추출물 제조방법 |
CN108531440A (zh) * | 2018-04-28 | 2018-09-14 | 刘汉石 | 一种人参细胞培养基及其应用 |
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KR100992800B1 (ko) * | 2010-05-14 | 2010-11-08 | 주식회사 지씨에이치앤피 | 미량의 진세노사이드 성분이 증가된 신규한 가공인삼 또는 가공인삼추출물의 제조방법 |
CN103609439B (zh) * | 2013-11-02 | 2015-12-09 | 吉林农业大学 | 不同菌种对人参发状根的影响及应用方法 |
CN105755090B (zh) * | 2016-03-18 | 2020-12-15 | 郭志刚 | 利用竹节参细胞大规模培养和生物转化技术获得竹节参皂苷等次生代谢产物的方法 |
CN106282291B (zh) * | 2016-10-19 | 2019-02-05 | 江西中天医药生物有限公司 | 细胞悬浮培养法生产人参皂甙的方法 |
US11499134B2 (en) * | 2019-01-24 | 2022-11-15 | Shenzhen Xiansheng Science And Technology Development Co., Ltd. | Method for culturing ginseng cell with high content of ginsenoside |
CN112899216A (zh) * | 2021-01-28 | 2021-06-04 | 深圳先声科技发展有限公司 | 一种人参皂苷含量高的人参细胞培养方法 |
-
2021
- 2021-01-28 CN CN202110120858.1A patent/CN112899216A/zh active Pending
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2022
- 2022-01-27 WO PCT/CN2022/074383 patent/WO2022161442A1/zh unknown
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CN102302420A (zh) * | 2011-07-29 | 2012-01-04 | 金凤燮 | 红参皂苷Rg2组和Rh1组、制备方法及在制备抗皮肤老化化妆品中的应用 |
WO2016028129A1 (ko) * | 2014-08-22 | 2016-02-25 | 주식회사 운화 | 희귀 진세노사이드를 고함유하는, 산삼 또는 인삼을 포함하는 인삼류 추출물, 인삼류 형성층 유래 식물줄기세포 또는 이의 추출물 제조방법 |
CN108531440A (zh) * | 2018-04-28 | 2018-09-14 | 刘汉石 | 一种人参细胞培养基及其应用 |
CN109576209A (zh) * | 2019-01-24 | 2019-04-05 | 深圳先声科技发展有限公司 | 人参形成层干细胞分离培养方法 |
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Title |
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J GINSENG RES 等: "Ginsenoside Production and Morphological Characterization of Wild Ginseng (Panax ginseng Meyer) Mutant Lines Induced by γ-irradiation ((60)Co) of Adventitious Roots", 《J GINSENG RES》 * |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022161442A1 (zh) * | 2021-01-28 | 2022-08-04 | 深圳先声科技发展有限公司 | 一种人参皂苷含量高的人参细胞培养方法 |
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Inventor after: Wu Dong Inventor after: Chen Huaide Inventor after: Cheng Lili Inventor after: Liu Minxian Inventor after: Hou Lili Inventor after: Liu Yujia Inventor after: Ling Yuanjian Inventor before: Hou Lili Inventor before: Chen Huaide Inventor before: Liu Minxian Inventor before: Liu Yujia Inventor before: Ling Yuanjian |
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