CN112675357A - Skin hydrogel dressing - Google Patents
Skin hydrogel dressing Download PDFInfo
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- CN112675357A CN112675357A CN202011645216.5A CN202011645216A CN112675357A CN 112675357 A CN112675357 A CN 112675357A CN 202011645216 A CN202011645216 A CN 202011645216A CN 112675357 A CN112675357 A CN 112675357A
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Abstract
The invention discloses a skin hydrogel dressing which comprises methacrylated gelatin and metal ions, wherein the metal ions are at least one of zinc ions, copper ions and manganese ions. In the skin hydrogel dressing, the methacrylic acid esterified gelatin and metal ions are innovatively used in a matched mode, and the apple stem cell extract, the growth factor TGF-beta 1, the radix tetrastigme extract, the thrombin freeze-dried powder and the like are combined to achieve the effects of repairing wounds and tightening skin.
Description
Technical Field
The invention relates to a skin hydrogel dressing.
Background
The hydrogel has high water content and rich physical and chemical properties, and has important application value in the field of biomedicine. The three-dimensional hydrogel dressing has a controllable structure, size and pores, and can provide a proper microenvironment for proliferation and differentiation of cells, so that repair and regeneration of tissues are efficiently realized. In recent years, the 3D-based printing technology (also referred to as additive manufacturing) has a significant advantage in structural modeling, which shows great potential in constructing complex three-dimensional hydrogel dressings, but currently photocrosslinked hydrogel printing systems are limited in biocompatibility and bioactivity.
Disclosure of Invention
In view of the above, it is an object of the present invention to overcome the above-mentioned disadvantages of the prior art and to provide a skin hydrogel dressing.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a skin hydrogel dressing comprising methacrylated gelatin and a metal ion, the metal ion being at least one of zinc ion, copper ion, manganese ion. The metal ions in the application can well promote osteogenesis, chondrogenesis, antibiosis and the like.
Preferably, the skin hydrogel dressing comprises the following components in parts by weight: 100 parts of methacrylated gelatin and 0.0001-1 part of metal ions.
Preferably, the skin hydrogel dressing further comprises an apple stem cell extract.
The apple stem cells in the application can rapidly activate dormant fibroblasts, promote cell division and proliferation, improve the cell DNA damage repair capacity, promote cell self-repair and renewal, enable skin cells to regenerate, continuously replace aged cells, and endow skin with sufficient moisture and super-strong water locking capacity; on the other hand, the apple stem cell extract can better promote the absorption of other components.
More preferably, the weight part of the apple stem cell extract is 5-10 parts.
Preferably, the skin hydrogel dressing is uniformly loaded with a growth factor TGF-beta 1, and the weight of the growth factor TGF-beta 1 is 1-3 parts.
More preferably, the skin hydrogel dressing further comprises 5-10 parts by weight of a radix tetrastigme extract. The radix tetrastigme extract has the good effects of clearing away heat and toxic materials, dispelling wind and eliminating phlegm, promoting blood circulation and relieving pain and the like.
Preferably, the skin hydrogel dressing further comprises 1-5 parts by weight of thrombin freeze-dried powder.
In the application, the apple stem cell extract, the metal ions, the radix tetrastigme extract and the thrombin freeze-dried powder can play a synergistic role together, and the effects of repairing wounds and tightening skin are better.
More preferably, the weight ratio of the metal ions, the apple stem cell extract, the radix tetrastigme extract and the thrombin freeze-dried powder is as follows: metal ions: apple stem cell extract: radix tetrastigme extract: the thrombin freeze-dried powder is 0.001:6:8: 3.
The four components in the weight ratio are compounded, so that the effects of repairing wounds and tightening skin can be better achieved.
Compared with the prior art, the invention has the beneficial effects that:
in the skin hydrogel dressing, the methacrylic acid esterified gelatin and metal ions are innovatively used in a matched mode, and the apple stem cell extract, the growth factor TGF-beta 1, the radix tetrastigme extract, the thrombin freeze-dried powder and the like are combined to achieve the effects of repairing wounds and tightening skin.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
In an embodiment of the skin hydrogel dressing of the present invention, the skin hydrogel dressing of the present invention is uniformly loaded with growth factor TGF- β 1, and at the same time, comprises the following components in parts by weight:
100 parts of methacrylated gelatin, 0.0001 part of metal ions (zinc ions), 5 parts of apple stem cell extract, 11 parts of growth factor TGF-beta, 5 parts of radix tetrastigme extract and 1 part of thrombin freeze-dried powder.
Example 2
In an embodiment of the skin hydrogel dressing of the present invention, the skin hydrogel dressing of the present invention is uniformly loaded with growth factor TGF- β 1, and at the same time, comprises the following components in parts by weight:
100 parts of methacrylated gelatin, 1 part of metal ions (copper ions), 10 parts of apple stem cell extract, 13 parts of growth factor TGF-beta, 10 parts of radix tetrastigme extract and 5 parts of thrombin freeze-dried powder.
Example 3
In an embodiment of the skin hydrogel dressing of the present invention, the skin hydrogel dressing of the present invention is uniformly loaded with growth factor TGF- β 1, and at the same time, comprises the following components in parts by weight:
100 parts of methacrylated gelatin, 0.1 part of metal ions (manganese ions), 8 parts of apple stem cell extract, 12 parts of growth factor TGF-beta, 6 parts of radix tetrastigme extract and 4 parts of thrombin freeze-dried powder.
Example 4
In an embodiment of the skin hydrogel dressing of the present invention, the skin hydrogel dressing of the present invention is uniformly loaded with growth factor TGF- β 1, and at the same time, comprises the following components in parts by weight:
100 parts of methacrylated gelatin, 0.5 part of metal ions (zinc ions), 9 parts of apple stem cell extract, 12 parts of growth factor TGF-beta, 7 parts of radix tetrastigme extract and 2 parts of thrombin freeze-dried powder.
Example 5
In an embodiment of the skin hydrogel dressing of the present invention, the skin hydrogel dressing of the present invention is uniformly loaded with growth factor TGF- β 1, and at the same time, comprises the following components in parts by weight:
100 parts of methacrylated gelatin, 0.001 part of metal ions (zinc ions), 6 parts of apple stem cell extract, 12 parts of growth factor TGF-beta, 8 parts of radix tetrastigme extract and 3 parts of thrombin freeze-dried powder.
Example 6
Effect of skin hydrogel dressing on wound healing in rats.
1. Experimental methods
The experimental rats totally contain 32 rats, the weight of the experimental rats ranges from 200 g to 250g, the experimental rats are male, the experimental rats are randomly divided into 8 groups, and each group contains 4 rats. After the rats are bred for 3 days conventionally, 2% sodium pentobarbital is injected into the abdominal cavity, the injection amount is 30mL/kg, after anesthesia is successful, the hairs in the back area are cut off by scissors, and the hairs are further removed by depilatory cream. A circular, full-thickness skin of 1.5cm diameter was then surgically removed to form an open wound, reaching the fascia. After the wound model is established, 8 groups of the dressing respectively correspond to the dressings in the examples 1-5, the comparative example 1 is antibacterial gauze, the comparative examples 2 and 3 only contain one of methacrylate gelatin and metal ions, and the rest is the same as the example 5. The mice of each group are respectively applied with corresponding dressing and then bandaged and fixed by gauze. The rats are raised in a single cage, the dressing change is carried out every two days for the first four days, the dressing change is carried out every three days later until the wound is healed, and the wound area and the recovery condition are calculated by software after the shooting in the period.
2. Results of the experiment
At 3 days, more cell exudates are present on each wound surface, at 7 days, the area of the wound surface is obviously reduced, and the wound is basically recovered at 14 days. The wound repair is shown in table 1.
TABLE 1 Effect of different dressings on the recovery of wounds in rats
As can be seen from Table 1, the materials in the embodiments 1-5 of the present application contain both methacrylated gelatin and metal ions, and both have a good function of promoting wound healing; comparative examples 2 and 3 contain only one of methacrylated gelatin and metal ions, and do not have a good function of promoting wound healing; therefore, the skin hydrogel auxiliary material disclosed by the application has the advantages that the methacrylated gelatin and the metal ions act together to play a synergistic effect, and a better effect of promoting wound healing is achieved.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (8)
1. The skin hydrogel dressing is characterized by comprising methacrylated gelatin and metal ions, wherein the metal ions are at least one of zinc ions, copper ions and manganese ions.
2. The skin hydrogel dressing of claim 1, comprising the following ingredients in parts by weight: 100 parts of methacrylated gelatin and 0.0001-1 part of metal ions.
3. The skin hydrogel dressing according to any one of claims 1 to 2, further comprising apple stem cell extract.
4. The skin hydrogel dressing according to claim 3, wherein the apple stem cell extract is 5 to 10 parts by weight.
5. The skin hydrogel dressing according to claim 3, wherein the growth factor TGF- β 1 is uniformly supported on the skin hydrogel dressing, and the weight of the growth factor TGF- β 1 is 1 to 3 parts.
6. The skin hydrogel dressing according to claim 5, further comprising 5 to 10 parts by weight of an extract of Hemsley rockvine root.
7. The skin hydrogel dressing according to claim 6, further comprising 1 to 5 parts by weight of thrombin lyophilized powder.
8. The skin hydrogel dressing of claim 7, wherein the metal ions, the apple stem cell extract, the radix tetrastigme extract, and the thrombin lyophilized powder are in a weight ratio of: metal ions: apple stem cell extract: radix tetrastigme extract: the thrombin freeze-dried powder is 0.001:6:8: 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011645216.5A CN112675357A (en) | 2020-12-31 | 2020-12-31 | Skin hydrogel dressing |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011645216.5A CN112675357A (en) | 2020-12-31 | 2020-12-31 | Skin hydrogel dressing |
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| CN112675357A true CN112675357A (en) | 2021-04-20 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462370A (en) * | 2022-11-03 | 2022-12-13 | 能科达(上海)干细胞研究中心有限公司 | Gel solution for preserving umbilical cord near totipotent stem cells and application thereof |
| CN116077721A (en) * | 2023-02-20 | 2023-05-09 | 深圳锋顺生物科技有限公司 | Gelatin-metal ion injectable hydrogel and preparation method and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102198287A (en) * | 2011-02-28 | 2011-09-28 | 北京上惠好生物技术有限公司 | Medical hydrogel and production technology |
| CN102740863A (en) * | 2009-11-06 | 2012-10-17 | 开尔弗森2200有限公司 | Copper salts of ion exchange materials for use in the treatment and prevention of infections |
| WO2014205261A1 (en) * | 2013-06-19 | 2014-12-24 | The Brigham And Women's Hospital, Inc. | Nanocomposite hydrogels |
| CN104788583A (en) * | 2014-01-17 | 2015-07-22 | 北京朗嘉仪生物技术有限公司 | Photo-crosslinking material, hydrogel formed by photo-crosslinking material and preparation method and application thereof |
| CN105055448A (en) * | 2015-05-25 | 2015-11-18 | 中国人民解放军第三军医大学第一附属医院 | Uses of copper ion preparation in preparation of drug or dressing for promoting skin keratinocyte to proliferate |
| CN106390188A (en) * | 2016-04-01 | 2017-02-15 | 浙江理工大学 | Hydrogel having wound repair function and preparation method of hydrogel |
| CN107383290A (en) * | 2017-08-09 | 2017-11-24 | 成都美益达医疗科技有限公司 | A kind of polyacrylate grafting modified gelatin adhesive and preparation method thereof |
| CN107802878A (en) * | 2017-09-06 | 2018-03-16 | 华南理工大学 | A kind of modified gelatin/potassium-sodium niobate compound electroactive antibacterial biological dressing and its preparation and application |
| CN109513038A (en) * | 2018-12-14 | 2019-03-26 | 华南理工大学 | The temperature-sensitive hydrogel and preparation method thereof of supported copper metal organic framework nanoparticle |
| CN109966557A (en) * | 2018-12-05 | 2019-07-05 | 博志生物科技有限公司 | The preparation method and application of the GelMA hydrogel of local sustained release Abaloparatide or related polypeptide |
| CN111533940A (en) * | 2020-04-11 | 2020-08-14 | 东华大学 | Tannic acid functionalized photo-crosslinked hydrogel stent and preparation method thereof |
-
2020
- 2020-12-31 CN CN202011645216.5A patent/CN112675357A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102740863A (en) * | 2009-11-06 | 2012-10-17 | 开尔弗森2200有限公司 | Copper salts of ion exchange materials for use in the treatment and prevention of infections |
| CN102198287A (en) * | 2011-02-28 | 2011-09-28 | 北京上惠好生物技术有限公司 | Medical hydrogel and production technology |
| WO2014205261A1 (en) * | 2013-06-19 | 2014-12-24 | The Brigham And Women's Hospital, Inc. | Nanocomposite hydrogels |
| CN104788583A (en) * | 2014-01-17 | 2015-07-22 | 北京朗嘉仪生物技术有限公司 | Photo-crosslinking material, hydrogel formed by photo-crosslinking material and preparation method and application thereof |
| CN105055448A (en) * | 2015-05-25 | 2015-11-18 | 中国人民解放军第三军医大学第一附属医院 | Uses of copper ion preparation in preparation of drug or dressing for promoting skin keratinocyte to proliferate |
| CN106390188A (en) * | 2016-04-01 | 2017-02-15 | 浙江理工大学 | Hydrogel having wound repair function and preparation method of hydrogel |
| CN107383290A (en) * | 2017-08-09 | 2017-11-24 | 成都美益达医疗科技有限公司 | A kind of polyacrylate grafting modified gelatin adhesive and preparation method thereof |
| CN107802878A (en) * | 2017-09-06 | 2018-03-16 | 华南理工大学 | A kind of modified gelatin/potassium-sodium niobate compound electroactive antibacterial biological dressing and its preparation and application |
| CN109966557A (en) * | 2018-12-05 | 2019-07-05 | 博志生物科技有限公司 | The preparation method and application of the GelMA hydrogel of local sustained release Abaloparatide or related polypeptide |
| CN109513038A (en) * | 2018-12-14 | 2019-03-26 | 华南理工大学 | The temperature-sensitive hydrogel and preparation method thereof of supported copper metal organic framework nanoparticle |
| CN111533940A (en) * | 2020-04-11 | 2020-08-14 | 东华大学 | Tannic acid functionalized photo-crosslinked hydrogel stent and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| IFFAT JAHAN ET AL: "Silver-Nanoparticle-Entrapped Soft GelMA Gels as Prospective Scaffolds for Wound Healing", 《AMERICAN CHEMICAL SOCIETY》 * |
| 培冈等著: "《带着我去化妆品柜台 2016全面升级版》", 31 December 2015, 新世界出版社 * |
| 胡馨尹著: "《三叶青预防和治疗肿瘤》", 31 August 2008, 中医古籍出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462370A (en) * | 2022-11-03 | 2022-12-13 | 能科达(上海)干细胞研究中心有限公司 | Gel solution for preserving umbilical cord near totipotent stem cells and application thereof |
| CN116077721A (en) * | 2023-02-20 | 2023-05-09 | 深圳锋顺生物科技有限公司 | Gelatin-metal ion injectable hydrogel and preparation method and application thereof |
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