CN112585271A - 用于生产udp-糖基转移酶的重组枯草芽孢杆菌及其重组方法 - Google Patents
用于生产udp-糖基转移酶的重组枯草芽孢杆菌及其重组方法 Download PDFInfo
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- CN112585271A CN112585271A CN201980055284.5A CN201980055284A CN112585271A CN 112585271 A CN112585271 A CN 112585271A CN 201980055284 A CN201980055284 A CN 201980055284A CN 112585271 A CN112585271 A CN 112585271A
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Abstract
一株用于生产UDP‑糖基转移酶的重组枯草芽孢杆菌及其重组方法，化学合成UDP‑糖基转移酶基因UGT，并与载体pUC57连接获得pUC57‑UGT，克隆不同启动子；所得的pUC57‑UGT与启动子连接到表达载体上，获得重组质粒；所得的重组质粒转化到宿主菌，获得宿主菌重组质粒；所得宿主菌重组质粒分别转化到枯草芽孢杆菌得到重组菌；所得重组菌筛选出高表达UDP‑糖基转移酶的重组枯草芽孢杆菌。通过将UDP‑糖基转移酶基因与不同启动子连接后转入枯草芽孢杆菌获得重组菌，并筛选出能够高效分泌表达UDP‑糖基转移酶的重组枯草芽孢杆菌，其分泌于胞外的UGT可以将莱胞迪苷A转化为莱胞迪苷D。
Description
PCT国内申请，说明书已公开。
Claims (8)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2019104167459 | 2019-05-20 | ||
| CN201910416745 | 2019-05-20 | ||
| PCT/CN2019/095986 WO2020232814A1 (zh) | 2019-05-20 | 2019-07-15 | 用于生产udp-糖基转移酶的重组枯草芽孢杆菌及其重组方法 |
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| Publication Number | Publication Date |
|---|---|
| CN112585271A true CN112585271A (zh) | 2021-03-30 |
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| CN201980055284.5A Pending CN112585271A (zh) | 2019-05-20 | 2019-07-15 | 用于生产udp-糖基转移酶的重组枯草芽孢杆菌及其重组方法 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20220064608A1 (zh) |
| CN (1) | CN112585271A (zh) |
| WO (1) | WO2020232814A1 (zh) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120164678A1 (en) * | 2010-11-30 | 2012-06-28 | Massachusetts Institute Of Technology | Microbial production of natural sweeteners, diterpenoid steviol glycosides |
| CN106795523A (zh) * | 2014-08-19 | 2017-05-31 | 可口可乐公司 | 制备莱鲍迪苷i的方法以及用途 |
| CN107109453A (zh) * | 2014-11-05 | 2017-08-29 | 马努斯生物合成股份有限公司 | 甜菊醇糖苷的微生物产生 |
| CN108893439A (zh) * | 2018-06-29 | 2018-11-27 | 江南大学 | 一种可高效表达葡萄糖脱氢酶的枯草芽胞杆菌工程菌 |
-
2019
- 2019-07-15 WO PCT/CN2019/095986 patent/WO2020232814A1/zh active Application Filing
- 2019-07-15 CN CN201980055284.5A patent/CN112585271A/zh active Pending
-
2021
- 2021-11-19 US US17/531,703 patent/US20220064608A1/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120164678A1 (en) * | 2010-11-30 | 2012-06-28 | Massachusetts Institute Of Technology | Microbial production of natural sweeteners, diterpenoid steviol glycosides |
| CN106795523A (zh) * | 2014-08-19 | 2017-05-31 | 可口可乐公司 | 制备莱鲍迪苷i的方法以及用途 |
| CN107109453A (zh) * | 2014-11-05 | 2017-08-29 | 马努斯生物合成股份有限公司 | 甜菊醇糖苷的微生物产生 |
| CN108893439A (zh) * | 2018-06-29 | 2018-11-27 | 江南大学 | 一种可高效表达葡萄糖脱氢酶的枯草芽胞杆菌工程菌 |
Non-Patent Citations (4)
| Title |
|---|
| NOGUCHI，A. 等: "AB360633.1" * |
| NOGUCHI，A. 等: "BAG80557.1" * |
| NOGUCHI，A. 等: "cDNA cloning of glycosyltransferases from Chinese wolfberry (Lycium barbarum L.) fruits and enzymatic synthesis of a catechin glucoside using a recombinant enzyme (UGT73A10)" * |
| 王振华 等: "芽孢杆苗的表达系统的研宄进展" * |
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| US20220064608A1 (en) | 2022-03-03 |
| WO2020232814A1 (zh) | 2020-11-26 |
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