CN112534051B - 基因组编辑方法、组合物、细胞、细胞制剂、以及细胞制剂的制造方法 - Google Patents

基因组编辑方法、组合物、细胞、细胞制剂、以及细胞制剂的制造方法

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Publication number
CN112534051B
CN112534051B CN201980034209.0A CN201980034209A CN112534051B CN 112534051 B CN112534051 B CN 112534051B CN 201980034209 A CN201980034209 A CN 201980034209A CN 112534051 B CN112534051 B CN 112534051B
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cells
homologous recombination
dna
cell
genome
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CN112534051A (zh
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花园丰
原弘真
鱼崎英毅
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Jichi Medical University
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Jichi Medical University
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Health & Medical Sciences (AREA)
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  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
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CN201980034209.0A 2018-03-29 2019-03-28 基因组编辑方法、组合物、细胞、细胞制剂、以及细胞制剂的制造方法 Active CN112534051B (zh)

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JP2018066174 2018-03-29
JP2018-066174 2018-03-29
PCT/JP2019/013602 WO2019189573A1 (ja) 2018-03-29 2019-03-28 ゲノム編集方法、組成物、細胞、細胞製剤、及び細胞製剤の製造方法

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CN112534051B true CN112534051B (zh) 2025-07-29

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US (1) US20210071202A1 (https=)
EP (1) EP3778888A4 (https=)
JP (1) JP7469806B2 (https=)
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WO (1) WO2019189573A1 (https=)

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CN116162621B (zh) * 2021-11-25 2025-09-16 中国科学院大连化学物理研究所 Dna双链序列及应用和肺腺癌细胞增殖抑制剂或在肺腺癌药物

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN107787367A (zh) * 2015-04-06 2018-03-09 里兰斯坦福初级大学理事会 用于crispr/cas介导的基因调控的化学修饰的引导rna

Family Cites Families (6)

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ES2731437T3 (es) 2014-11-21 2019-11-15 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida mediante el uso de pares de ARN guías
US20180119174A1 (en) * 2015-05-13 2018-05-03 Seattle Children's Hospita (dba Seattle Children's Research Institute Enhancing endonuclease based gene editing in primary cells
JP6937740B2 (ja) * 2015-07-28 2021-09-22 ダニスコ・ユーエス・インク ゲノム編集システムおよび使用方法
WO2017096041A1 (en) * 2015-12-02 2017-06-08 The Regents Of The University Of California Compositions and methods for modifying a target nucleic acid
JP6776090B2 (ja) 2016-10-19 2020-10-28 アルプスアルパイン株式会社 位置判定装置、位置判定方法及びプログラム並びにキーレスエントリーシステム
US11866726B2 (en) * 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107787367A (zh) * 2015-04-06 2018-03-09 里兰斯坦福初级大学理事会 用于crispr/cas介导的基因调控的化学修饰的引导rna

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage;Jian-Ping Zhang等;Genome Biology;20170220;第18卷(第1期);第4页左栏第2段、图2 *
Hematopoietic stem cell gne therapy:progress and lesions learned;Richard A Morgan等;cell stem cell;20171102;第21卷(第5期);摘要、第9页最后1段、图3 *

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EP3778888A1 (en) 2021-02-17
JP7469806B2 (ja) 2024-04-17
WO2019189573A1 (ja) 2019-10-03
CN112534051A (zh) 2021-03-19
US20210071202A1 (en) 2021-03-11
JPWO2019189573A1 (ja) 2021-04-15
EP3778888A4 (en) 2022-04-13

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