CN112500239A - Plant protective agent and preparation method thereof - Google Patents
Plant protective agent and preparation method thereof Download PDFInfo
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- CN112500239A CN112500239A CN202011162919.2A CN202011162919A CN112500239A CN 112500239 A CN112500239 A CN 112500239A CN 202011162919 A CN202011162919 A CN 202011162919A CN 112500239 A CN112500239 A CN 112500239A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Pest Control & Pesticides (AREA)
- Genetics & Genomics (AREA)
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- Biomedical Technology (AREA)
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- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
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Abstract
The invention discloses a plant protective agent, which comprises a compound microbial agent and a fertilizer, wherein the mass ratio of the compound microbial agent to the fertilizer is 10-50: 50-90; the compound microbial agent comprises the following raw materials in parts by weight: 2-10 parts of streptomyces jingyangensis, 5-15 parts of bacillus subtilis, 5-15 parts of bacillus amyloliquefaciens, 1-6 parts of rhodospirillum and 1-6 parts of rhizobia. The plant protective agent has high efficiency, low toxicity, environmental protection, safety and long lasting period, can effectively improve the quality and safety of food, and is beneficial to the sustainable development of the environment. In addition, the invention also provides a preparation method of the plant protective agent, and the method also has the technical effects, and is simple in process and low in production cost.
Description
Technical Field
The invention relates to the technical field of plant protection, in particular to a plant protective agent and a preparation method thereof.
Background
The pesticide is a chemical agent used for preventing and controlling plant diseases and insect pests and regulating plant growth in agriculture, and is widely used for agriculture, forestry and animal husbandry production, environmental and household sanitation, pest and disease control, industrial product mildew and moth prevention and the like. The pesticide has various varieties, and can be mainly divided into insecticide, acaricide, rodenticide, nematicide, molluscicide, bactericide, herbicide, plant growth regulator and the like according to the application; according to the raw material source, the pesticide can be divided into mineral source pesticides (inorganic pesticides), biological source pesticides (natural organic matters, microorganisms, antibiotics and the like) and chemical synthetic pesticides; according to the chemical structure, organic chlorine, organic phosphorus, organic nitrogen, organic sulfur, carbamate, pyrethroid, amide compounds, urea compounds, ether compounds, phenol compounds, phenoxy carboxylic acids, amidines, triazoles, heterocycles, benzoic acids, organometallic compounds and the like are mainly used.
As a big agricultural country, the pesticide plays an important role in agricultural production in China. With the improvement of health consciousness of people, consumers pay more and more attention to the safety of agricultural products, and the national restrictions on pesticide residues are more and more strict. In order to reduce the use risk of pesticides and reduce pesticide residues, efforts to develop novel pesticides have become urgent.
Disclosure of Invention
In order to solve the technical problems, the invention provides a plant protective agent which has high efficiency, low toxicity, environmental protection, safety and long lasting period, can effectively improve the quality and safety of food, and is beneficial to the sustainable development of the environment. In addition, the invention also provides a preparation method of the plant protective agent, and the method also has the technical effects, and is simple in process and low in production cost.
The invention discloses a plant protective agent, which comprises a compound microbial agent and a fertilizer, wherein the mass ratio of the compound microbial agent to the fertilizer is 10-50: 50-90; the compound microbial agent comprises the following raw materials in parts by weight: 2-10 parts of streptomyces jingyangensis, 5-15 parts of bacillus subtilis, 5-15 parts of bacillus amyloliquefaciens, 1-6 parts of rhodospirillum and 1-6 parts of rhizobia.
Preferably, the fertilizer comprises the following raw materials in parts by weight: 16-18 parts of urea, 27-30 parts of ammonium bicarbonate, 7-11 parts of calcium superphosphate, 6-20 parts of potassium chloride, 8-14 parts of amino acid, 30-40 parts of plant ash and 15-25 parts of buckwheat hulls.
Preferably, the preparation method of the streptomyces jingyangensis comprises the steps of inoculating 4-6 wt% of streptomyces jingyangensis into a first culture medium for fermentation culture at the temperature of 25-35 ℃ for 4-8 days, and drying and crushing at low temperature after fermentation; the first culture medium comprises the following raw materials in parts by weight: 5-10 parts of soybean meal, 5-8 parts of rice bran powder, 2-4 parts of soybean powder, 6-8 parts of corn flour, 0.2-0.45 part of ammonium bicarbonate, 5-15 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.1-1 part of magnesium sulfate, 0.1-0.5 part of sodium propionate, 0.1-0.4 part of ammonium nitrate and 80-90 parts of sterile water.
Preferably, the preparation method of the bacillus subtilis comprises the steps of inoculating 2-5 wt% of bacillus subtilis into a second culture medium for fermentation culture at 28-33 ℃ for 2-7 days, and drying and crushing at low temperature after fermentation; the second culture medium comprises the following raw materials in parts by weight: 8-12 parts of soybean meal, 3-6 parts of rice bran powder, 5-9 parts of corn flour, 0.5-2 parts of ammonium sulfate, 5-15 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.1-1 part of magnesium sulfate, 1.5-3 parts of monopotassium phosphate, 2-4 parts of urea and 80-90 parts of sterile water.
Preferably, the preparation method of the bacillus amyloliquefaciens comprises the steps of inoculating 1-3 wt% of bacillus amyloliquefaciens into a third culture medium for fermentation culture at the temperature of 25-33 ℃ for 2-3 days, and drying and crushing at low temperature after fermentation; the third culture medium comprises the following raw materials in parts by weight: 5-10 parts of seaweed ash, 5-8 parts of rice bran powder, 2-5 parts of yeast powder, 0.2-0.45 part of ammonium bicarbonate, 15-20 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.5-2 parts of magnesium sulfate, 0.1-0.4 part of ammonium nitrate and 200 parts of sterile water 150-.
Preferably, the preparation method of the rhodospirillum comprises the steps of inoculating 8-12 wt% of rhodospirillum in a fourth culture medium for anaerobic fermentation culture at the temperature of 26-32 ℃ for 5-8 days, and drying and crushing at low temperature after fermentation; the fourth culture medium comprises the following raw materials in parts by weight: 2-5 parts of yeast extract, 5-10 parts of soybean meal, 3-5 parts of corn flour, 4-8 parts of glucose, 5-6 parts of disodium hydrogen phosphate, 0.5-0.8 part of magnesium sulfate and 85-95 parts of sterile water. .
Preferably, the rhizobium is prepared by inoculating 0.5-1.2 wt% of rhizobium to a fifth culture medium for fermentation culture at 22-26 ℃ for 3-4 days, and drying and crushing at low temperature after fermentation; the fifth culture medium comprises the following raw materials in parts by weight: 5-10 parts of soybean meal powder, 5-8 parts of rice bran powder, 8-10 parts of mannitol, 5-15 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.5-0.8 part of magnesium sulfate and 120 parts of sterile water 100.
In addition, the invention also provides a preparation method of the plant protective agent, which comprises the following steps: the compound microbial agent and the fertilizer are mixed according to the proportion, and the finished product is obtained after the mixture is crushed at room temperature and is sieved by a 200-micron 350-micron pore size sieve.
Compared with the prior art, the plant protective agent provided by the invention has the beneficial effects that: the obvious synergistic effect generated by combining a plurality of active components in the plant protective agent is utilized, the use times of the plant protective agent are reduced, the use cost is reduced, the pollution to the environment is reduced, the generation of drug resistance of pests is delayed, and the service life of the plant protective agent is prolonged.
Compared with the prior art, the insecticidal composition has reasonable components, is safe and nontoxic, can be naturally degraded, has good effect of controlling pests, and low dosage and cost, and has obvious synergistic effect instead of simple superposition of the components.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Example 1
A plant protective agent comprises a compound microbial agent and a fertilizer, wherein the mass ratio of the compound microbial agent to the fertilizer is 10: 90; the compound microbial agent comprises the following raw materials in parts by weight: 2 parts of streptomyces jingyangensis, 15 parts of bacillus subtilis, 15 parts of bacillus amyloliquefaciens, 3 parts of rhodospirillum and 3 parts of rhizobia; the fertilizer comprises the following raw materials in parts by weight: 16 parts of urea, 27 parts of ammonium bicarbonate, 7 parts of calcium superphosphate, 12 parts of potassium chloride, 10 parts of amino acid, 35 parts of plant ash and 20 parts of buckwheat hulls.
Example 2
A plant protective agent comprises a compound microbial agent and a fertilizer, wherein the mass ratio of the compound microbial agent to the fertilizer is 50: 50; the compound microbial agent comprises the following raw materials in parts by weight: 6 parts of streptomyces jingyangensis, 8 parts of bacillus subtilis, 8 parts of bacillus amyloliquefaciens, 5 parts of rhodospirillum and 5 parts of rhizobia; the fertilizer comprises the following raw materials in parts by weight: 18 parts of urea, 29 parts of ammonium bicarbonate, 10 parts of calcium superphosphate, 15 parts of potassium chloride, 11 parts of amino acid, 34 parts of plant ash and 17 parts of buckwheat hulls.
Example 3
A plant protective agent comprises a compound microbial agent and a fertilizer, wherein the mass ratio of the compound microbial agent to the fertilizer is 40: 60; the compound microbial agent comprises the following raw materials in parts by weight: 6 parts of streptomyces jingyangensis, 13 parts of bacillus subtilis, 8 parts of bacillus amyloliquefaciens, 4 parts of rhodospirillum and 6 parts of rhizobia; the fertilizer comprises the following raw materials in parts by weight: 17 parts of urea, 29 parts of ammonium bicarbonate, 9 parts of calcium superphosphate, 16 parts of potassium chloride, 13 parts of amino acid, 16 parts of plant ash and 18 parts of buckwheat hulls.
The preparation method of the streptomyces jingyangensis comprises the steps of inoculating 4 wt% of streptomyces jingyangensis into a first culture medium for fermentation culture at the temperature of 25 ℃ for 6 days, and drying and crushing at low temperature after fermentation; the first culture medium comprises the following raw materials in parts by weight: 8 parts of soybean meal, 6 parts of rice bran powder, 3 parts of soybean meal, 7 parts of corn meal, 0.3 part of ammonium bicarbonate, 10 parts of glucose, 3 parts of disodium hydrogen phosphate, 0.5 part of magnesium sulfate, 0.4 part of sodium propionate, 0.2 part of ammonium nitrate and 85 parts of sterile water.
The preparation method of Bacillus subtilis comprises inoculating 3 wt% Bacillus subtilis to a second culture medium, fermenting at 30 deg.C for 5 days, drying at low temperature, and pulverizing; the second culture medium comprises the following raw materials in parts by weight: 10 parts of soybean meal powder, 4 parts of rice bran powder, 7 parts of corn flour, 1.5 parts of ammonium sulfate, 8 parts of glucose, 2 parts of disodium hydrogen phosphate, 0.6 part of magnesium sulfate, 2.5 parts of potassium dihydrogen phosphate, 4 parts of urea and 86 parts of sterile water.
The preparation method of the bacillus amyloliquefaciens comprises the steps of inoculating 2.5 wt% of bacillus amyloliquefaciens into a third culture medium for fermentation culture at 31 ℃ for 2 days, and drying and crushing at low temperature after fermentation; the third culture medium comprises the following raw materials in parts by weight: 8 parts of seaweed ash, 7 parts of rice bran powder, 3 parts of yeast powder, 0.3 part of ammonium bicarbonate, 17 parts of glucose, 2 parts of disodium hydrogen phosphate, 1.5 parts of magnesium sulfate, 0.25 part of ammonium nitrate and 160 parts of sterile water.
The preparation method of Rhodospirillum comprises inoculating 10 wt% of Rhodospirillum into fourth culture medium for anaerobic fermentation culture at 26 deg.C for 5 days, drying at low temperature, and pulverizing; the fourth culture medium comprises the following raw materials in parts by weight: 5 parts of yeast extract, 10 parts of soybean meal, 5 parts of corn flour, 6 parts of glucose, 5 parts of disodium hydrogen phosphate, 0.7 part of magnesium sulfate and 90 parts of sterile water. .
The rhizobium is prepared by inoculating 0.8 wt% of rhizobium to a fifth culture medium, fermenting at 25 deg.C for 4 days, and drying at low temperature and pulverizing; the fifth culture medium comprises the following raw materials in parts by weight: 6 parts of soybean meal powder, 8 parts of rice bran powder, 10 parts of mannitol, 12 parts of glucose, 3 parts of disodium hydrogen phosphate, 0.8 part of magnesium sulfate and 120 parts of sterile water.
Mixing the prepared compound microbial agent and fertilizer in proportion, crushing at room temperature, and sieving with a 250-micron pore size sieve to obtain the finished product.
The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same or similar parts in the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. A plant protective agent is characterized by comprising a compound microbial agent and a fertilizer, wherein the mass ratio of the compound microbial agent to the fertilizer is 10-50: 50-90;
the compound microbial agent comprises the following raw materials in parts by weight: 2-10 parts of streptomyces jingyangensis, 5-15 parts of bacillus subtilis, 5-15 parts of bacillus amyloliquefaciens, 1-6 parts of rhodospirillum and 1-6 parts of rhizobia.
2. The plant protection agent as claimed in claim 1, wherein the fertilizer comprises the following raw materials in parts by weight: 16-18 parts of urea, 27-30 parts of ammonium bicarbonate, 7-11 parts of calcium superphosphate, 6-20 parts of potassium chloride, 8-14 parts of amino acid, 30-40 parts of plant ash and 15-25 parts of buckwheat hulls.
3. The plant protectant of claim 1, wherein the streptomyces jingyangensis is prepared by inoculating 4-6 wt% streptomyces jingyangensis to a first culture medium, fermenting at 25-35 deg.C for 4-8 days, drying at low temperature, and pulverizing;
the first culture medium comprises the following raw materials in parts by weight: 5-10 parts of soybean meal, 5-8 parts of rice bran powder, 2-4 parts of soybean powder, 6-8 parts of corn flour, 0.2-0.45 part of ammonium bicarbonate, 5-15 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.1-1 part of magnesium sulfate, 0.1-0.5 part of sodium propionate, 0.1-0.4 part of ammonium nitrate and 80-90 parts of sterile water.
4. The plant protective agent according to claim 1, wherein the bacillus subtilis is prepared by inoculating 2-5 wt% of bacillus subtilis to a second medium, fermenting and culturing at 28-33 ℃ for 2-7 days, drying and pulverizing at low temperature after fermentation;
the second culture medium comprises the following raw materials in parts by weight: 8-12 parts of soybean meal, 3-6 parts of rice bran powder, 5-9 parts of corn flour, 0.5-2 parts of ammonium sulfate, 5-15 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.1-1 part of magnesium sulfate, 1.5-3 parts of monopotassium phosphate, 2-4 parts of urea and 80-90 parts of sterile water.
5. The plant protective agent according to claim 1, wherein the bacillus amyloliquefaciens is prepared by inoculating 1-3 wt% of bacillus amyloliquefaciens to a third culture medium, fermenting and culturing at 25-33 ℃ for 2-3 days, drying at low temperature and pulverizing after fermenting;
the third culture medium comprises the following raw materials in parts by weight: 5-10 parts of seaweed ash, 5-8 parts of rice bran powder, 2-5 parts of yeast powder, 0.2-0.45 part of ammonium bicarbonate, 15-20 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.5-2 parts of magnesium sulfate, 0.1-0.4 part of ammonium nitrate and 200 parts of sterile water 150-.
6. The plant protective agent according to claim 1, wherein the rhodospirillum is prepared by inoculating 8-12 wt% of rhodospirillum to a fourth medium for anaerobic fermentation culture at 26-32 ℃ for 5-8 days, drying and pulverizing at low temperature after fermentation;
the fourth culture medium comprises the following raw materials in parts by weight: 2-5 parts of yeast extract, 5-10 parts of soybean meal, 3-5 parts of corn flour, 4-8 parts of glucose, 5-6 parts of disodium hydrogen phosphate, 0.5-0.8 part of magnesium sulfate and 85-95 parts of sterile water.
7. The plant protecting agent as claimed in claim 1, wherein the rhizobia is prepared by inoculating 0.5-1.2 wt% rhizobia to a fifth medium, fermenting at 22-26 deg.C for 3-4 days, drying at low temperature, and pulverizing;
the fifth culture medium comprises the following raw materials in parts by weight: 5-10 parts of soybean meal powder, 5-8 parts of rice bran powder, 8-10 parts of mannitol, 5-15 parts of glucose, 2-4 parts of disodium hydrogen phosphate, 0.5-0.8 part of magnesium sulfate and 120 parts of sterile water 100.
8. The method for preparing the plant protective agent according to any one of claims 1 to 7, characterized in that the compound microbial agent and the fertilizer are mixed in proportion, and the mixture is crushed at room temperature and then sieved by a 200-350 micron pore size sieve to obtain a finished product.
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| CN202011162919.2A CN112500239A (en) | 2020-10-27 | 2020-10-27 | Plant protective agent and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011162919.2A CN112500239A (en) | 2020-10-27 | 2020-10-27 | Plant protective agent and preparation method thereof |
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| CN112500239A true CN112500239A (en) | 2021-03-16 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112457107A (en) * | 2020-12-11 | 2021-03-09 | 陈兰 | Plant nutrient solution |
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| CN110437000A (en) * | 2019-08-14 | 2019-11-12 | 潍坊市绿中新能源科技有限公司 | A kind of microbial manure and its preparation method and application |
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2020
- 2020-10-27 CN CN202011162919.2A patent/CN112500239A/en active Pending
Patent Citations (5)
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| CN104230580A (en) * | 2014-08-21 | 2014-12-24 | 上海亨康生物科技有限公司 | Composite microbial disease/insect-resistant agent for green vegetables and manufacturing method thereof |
| CN104472554A (en) * | 2014-12-31 | 2015-04-01 | 吴中区金庭小美华家庭农场 | Composite microbial preparation for preventing and treating white rust of rape and preparation method of composite microbial preparation |
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| CN109867565A (en) * | 2019-03-19 | 2019-06-11 | 中国热带农业科学院环境与植物保护研究所 | A kind of plant protection product and its application method |
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Application publication date: 20210316 |