CN112266861A - Oil reservoir microorganism storage and transportation device and application - Google Patents
Oil reservoir microorganism storage and transportation device and application Download PDFInfo
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Abstract
The invention provides an oil reservoir microorganism storage and transportation device and application. The apparatus comprises a pressure control system (1), a temperature control system (2), and a reactor system (3); pressure control system (1) includes force (forcing) pump (11) and pressure measurement device (12), temperature control system (2) includes thermostated container (21), reactor system (3) include one or more parallelly connected temperature resistant pressure-resistant container (31) that have floating piston, temperature resistant pressure-resistant container (31) bottom sets up first three-way valve (32), the top sets up second three-way valve (33), temperature resistant pressure-resistant container (31) can be connected with force (forcing) pump (11) through first three-way valve (32) and via the pipeline, and can be connected with pressure measurement device (12) respectively through second three-way valve (33) and via the pipeline. The device can perform the microorganism culture in a standing mode under the conditions of high temperature and high pressure, meets the requirements of multiple groups of parallel experiments, and ensures the repeatability of the experiments.
Description
Technical Field
The invention relates to the field of oil exploitation, in particular to an oil reservoir microorganism storage and transportation device and application thereof.
Background
Microbial Enhanced Oil Recovery (MEOR) is one of the most promising technologies for Enhanced Oil Recovery currently considered, and is mainly achieved by acting microorganisms or metabolites of microorganisms on Oil reservoirs to improve the mobility of crude Oil in the formation or by adjusting plugging to expand water flooding wave and volume. The MEOR technology has the advantages of low cost and environmental protection, and has unique development advantages in residual oil reservoirs. To date, the large-scale industrial application of microbial oil recovery technology has not been widely popularized, and one very important restriction factor is that the mechanism of improving oil recovery efficiency by microbes is not completely clear-the composition and influence factors of microbial communities in real oil reservoir environments are not fully and systematically known. Pressure is an important environmental factor of underground deep oil reservoirs, and related researches on pressure influencing microorganisms in the deep part of the earth are just started, and a plurality of important problems are required to be deeply researched. Although indoor studies on reservoir microorganisms have been carried out for decades, due to objective limitations on oil field sampling and transportation conditions, a large number of samples are conventionally sampled and mailed by oilfield workers, the commercial sampling and transportation process is rough compared with the precise study on micro-life reservoir microorganisms, and a large amount of community information is lost in the processes of sampling, container packaging, normal-pressure long-distance transportation and the like. In laboratory work, the real information of the oil deposit microorganisms is greatly lost by a common normal pressure culture mode. Therefore, the invention is necessary to invent a device system for oil deposit microorganism pressure-carrying transportation and high-temperature high-pressure culture.
Disclosure of Invention
By reducing the time for reducing the pressure after the oil deposit microorganisms leave the oil deposit as much as possible, the sample is ensured to be stored under the oil deposit pressure and in an anaerobic environment. Therefore, after sampling at the well mouth, the container is locally subjected to container exhaust, then is sealed, and is transported after pressure compensation, and the simulation of the high-temperature and high-pressure physical model environment of the oil reservoir is realized in a laboratory, so that more comprehensive real information of the oil reservoir microorganisms can be obtained. The method has high requirements on pressure-carrying transportation and high-temperature high-pressure culture of the oil reservoir microorganisms after sampling, and a device system for pressure-carrying transportation and high-temperature high-pressure culture of the oil reservoir microorganisms is necessary to design.
An object of the present invention is to provide an oil reservoir microorganism storage and transportation device; the device is used for pressure-carrying transportation and high-temperature high-pressure culture.
The device of the invention not only can carry out pressure-loading transportation on the oil deposit microorganism sample after pressure supplement after the oil deposit microorganism sample is extracted at the well head of the oil field, but also can carry out microorganism culture in a standing mode under the condition of high temperature and high pressure, thereby meeting the requirements of a plurality of groups of parallel experiments and ensuring the repeatability of the experiments. The change of injection pump and manometer dual detection pressure, the operation is safe convenient, is fit for the researcher of oil reservoir microorganism and uses.
Another object of the present invention is to provide a method for preserving microorganisms in an oil reservoir.
To achieve the above objects, in one aspect, the present invention provides an oil reservoir microorganism storage and transportation apparatus, wherein the apparatus comprises a pressure control system 1, a temperature control system 2, and a reactor system 3; pressure control system 1 includes force (forcing) pump 11 and pressure measurement device 12, temperature control system 2 includes thermostated container 21, reactor system 3 includes one or more parallelly connected temperature resistant pressure-resistant container 31 that has floating piston 311, temperature resistant pressure-resistant container 31 bottom sets up first three-way valve 32, and the top sets up second three-way valve 33, temperature resistant pressure-resistant container 31 can be connected with force (forcing) pump 11 through first three-way valve 32 and via the pipeline to can be connected with pressure measurement device 12 through second three-way valve 33 and via the pipeline respectively.
Storage and transportation of the present invention, including storage and/or transportation.
Even further, the storage and transportation may include high temperature and high pressure culturing of microorganisms.
According to some embodiments of the invention, the pressurizing pump 11 is a displacement pump.
According to some embodiments of the present invention, the pressurizing pump 11 is one or a plurality of pumps connected in parallel.
According to some embodiments of the present invention, the pressure pump 11 is connected in parallel, and the pipeline connected to the outlet 111 of the pressure pump 11 is converged at one point and then connected to the temperature-resistant pressure-resistant container 31.
According to some embodiments of the present invention, the pressurizing pumps 11 are two pumps connected in parallel.
According to some embodiments of the invention, a valve 112 is provided at the outlet 111 of each booster pump 11.
According to some embodiments of the invention, the displacement pump employs a coaxial structure, a dual motor controller and a high precision stepper motor.
According to some embodiments of the present invention, the incubator 21 further comprises a temperature probe 22 and a temperature alarm device 23.
According to some embodiments of the present invention, the temperature alarm device 23 alarms when the temperature in the incubator is within ± 3 ℃ of the experimental temperature.
According to some embodiments of the present invention, the maximum pressure of the pressurizing pump 11 is 69.6MPa, and the maximum flow rate is 15 ml/min.
According to some embodiments of the present invention, the pressure of the pressurizing pump 11 is in the range of 0.069 to 69.6 MPa.
According to some embodiments of the present invention, the continuous flow rate of the pressurizing pump 11 is in the range of 0.001-132 psi.
According to some embodiments of the present invention, the pressure measuring device 12 is a pressure gauge measuring 2.0-2.5 times the test pressure (so that the pointer position is near the center of the dial).
According to some embodiments of the present invention, the maximum pressure resistance of the temperature-resistant pressure-resistant container 31 is 70MPa, and the maximum temperature-resistant temperature is 200 ℃.
According to some specific embodiments of the present invention, the maximum pressure resistance and the maximum temperature resistance of the first three-way valve 32 and the second three-way valve 33 are respectively 70MPa and 200 ℃.
According to some embodiments of the present invention, the maximum pressure resistance and the maximum temperature resistance of the pipeline between the pressure pump 11, the first three-way valve 32, the second three-way valve 33, the pressure measuring device 12 and the temperature-resistant pressure-resistant container 31 are 70MPa and 200 ℃.
According to some embodiments of the present invention, the temperature and pressure resistant container 31 has a cylindrical shape, and the total volume of the chamber excluding the piston portion is 1L.
According to some embodiments of the present invention, the temperature-resistant and pressure-resistant containers 31 are connected in parallel, and the pipeline at the bottom of each temperature-resistant and pressure-resistant container 31 connected to the pressure pump is led out by the first three-way valve 32 and converged at a point, and then connected to the pressure pump 11.
According to some specific embodiments of the present invention, the temperature-resistant and pressure-resistant containers 31 are 2-6 in parallel.
According to some specific embodiments of the invention, the piston is close to the bottom of the temperature-resistant and pressure-resistant container; one side of the piston, which faces the top of the temperature-resistant pressure-resistant container, is in contact with sterile water injected through the displacement pump, and the other side of the piston is in contact with an oil reservoir microorganism sample for culture.
In a non-storage and transportation state, the pressure control system 1, the temperature control system 2 and the reactor system 3 of the invention can be connected or can be disassembled.
According to some embodiments of the present invention, during storage and transportation, the bottom of the temperature-resistant pressure-resistant container 31 is connected to the pressure pump 11 through a first three-way valve 32 and a pipeline, and the top of the temperature-resistant pressure-resistant container 31 is connected to the pressure measuring device 12 through a second three-way valve 33 and a pipeline, and the temperature-resistant pressure-resistant container 31 is disposed in the incubator 21.
According to some embodiments of the invention, the device further comprises a sampling system 4; the sampling system 4 comprises a metering container 41 and a buffer container 42; the top of the temperature-resistant pressure-resistant container 31 can be connected to the pressure measuring device 12 and the metering container 41 through a second three-way valve 33 and a pipeline.
According to some embodiments of the present invention, the sampling system 4 further comprises a gas collecting device 43 connected to the buffer container 42 via a pipeline.
According to some embodiments of the present invention, in the sampling state, the temperature-resistant pressure-resistant container 31 is connected to the pressurizing pump 11 through the first three-way valve 32 at the bottom, connected to the pressure measuring device 12 and the metering container 41 through the second three-way valve 33 at the top, and connected to the buffer container 42 through the pipeline, respectively.
According to some embodiments of the present invention, in the sampling state, the temperature-resistant pressure-resistant container 31 is kept in communication with the pressure pump 11 and the pressure measuring device 12, respectively (the first three-way valve 32 and the second three-way valve 33 are both open).
According to some embodiments of the present invention, the temperature-resistant and pressure-resistant containers 31 are multiple in parallel, and the pipeline on the top of each temperature-resistant and pressure-resistant container 31, which is connected to the metering container 41, is led out by the second three-way valve 32 and converged at a point, and then is connected to the metering container 41.
According to some specific embodiments of the present invention, the temperature-resistant and pressure-resistant containers 31 are 2-6 in parallel.
In another aspect, the present invention further provides a method for preserving microorganisms in an oil reservoir, wherein the method comprises the following steps:
sampling; and
storage and transportation steps: the collected microorganism samples are stored and transported by using the oil reservoir microorganism storage and transportation device.
According to some embodiments of the invention, the step of storing and transporting comprises:
placing the produced liquid containing the microorganism sample obtained in the sampling step into a temperature-resistant pressure-resistant container 31;
discharging and pressurizing gas in the temperature-resistant pressure-resistant container 31 to the pressure of an oil reservoir where produced liquid is located, and then closing the first three-way valve 32 and the second three-way valve 33;
a step of placing the temperature-resistant pressure-resistant container 31 in the incubator 21 and connecting with the pressure-increasing pump 11 through a first three-way valve 32 and via a pipeline, and connecting with the pressure-measuring device 12 through a second three-way valve 33 and via a pipeline, to control the pressure inside the temperature-resistant pressure-resistant container 31 with the pressure-increasing pump 11; and
a step of raising the temperature of the thermostat 21 to the reservoir temperature.
According to some embodiments of the present invention, the step of raising the temperature of the thermostat 21 to the reservoir temperature comprises a step-by-step temperature rise with an increase of 4-6 ℃/h, wherein each time the temperature rise is carried out until the pressure is stabilized, the temperature rise is further carried out.
According to some embodiments of the invention, the pressure stabilization refers to a pressure recovery to a set pressure after a temperature increase.
According to some embodiments of the invention, the method further comprises the step of sampling with the reservoir microorganism storage and transportation device of the invention.
Wherein the device further comprises a sampling system 4; the sampling system 4 comprises a metering container 41 and a buffer container 42; the top of the temperature-resistant pressure-resistant container 31 can be connected to the pressure measuring device 12 and the metering container 41 through a second three-way valve 33 and a pipeline.
According to some embodiments of the present invention, the sampling system 4 further comprises a gas collecting device 43 connected to the buffer container 42 via a pipeline.
According to some embodiments of the present invention, in the sampling state, the temperature-resistant pressure-resistant container 31 is connected to the pressurizing pump 11 through the first three-way valve 32 at the bottom, connected to the pressure measuring device 12 and the metering container 41 through the second three-way valve 33 at the top, and connected to the buffer container 42 through the pipeline, respectively.
According to some embodiments of the present invention, the sampling step includes driving a floating piston of the temperature-resistant and pressure-resistant container 31 to move by using the pressure pump 11 to inject the produced fluid into the metering container 41.
According to some embodiments of the present invention, the step of discharging the gas after releasing the pressure is further included after the produced fluid is injected into the metering container 41.
In conclusion, the invention provides an oil reservoir microorganism storage and transportation device and application. The device of the invention has the following advantages:
simple structure and reasonable technology. The floating piston type temperature-resistant pressure-resistant culture container enables the internal pressure of the whole container to be increased in a mode of pressurizing one end of the piston, and a microorganism sample cannot be polluted. Not only satisfies timely pressure compensation and pressure-carrying transportation after sampling, but also satisfies the real-time accurate control of pressure along with the change of biological metabolism during static culture. In addition, the pressure control is carried out in a liquid injection mode, the high-pressure safety is guaranteed, and meanwhile, a buffer device is additionally arranged at the sampling end, so that the safe discharge of a small amount of gas generated in biological metabolism is facilitated. The high-precision displacement pump and the pressure gauge are simultaneously detected, and particularly the displacement pump controls the pressure in real time, so that the oil reservoir microorganism culture under long-term stable and safe operation is ensured. In addition, the floating piston type temperature-resistant pressure-resistant culture container can be externally connected with a container to inject experimental additives under high pressure, and has great research and practical space.
Compared analysis of the oil reservoir microbial community structure after a group of oil field sample pressure-carrying transportation and normal pressure transportation shows that part of important microbial community information, especially the information of archaea community is reserved under high pressure; after long-term culture at high temperature and high pressure and at high temperature and normal pressure, genome sequencing comparative analysis shows that community distribution of bacteria and archaea is obviously affected by pressure.
Drawings
FIG. 1 is a schematic block diagram of an oil reservoir microorganism storage and transportation device of the present invention;
FIG. 2 is a schematic structural view of an oil reservoir microorganism storage and transportation apparatus according to example 1 of the present invention;
FIG. 3 is a schematic structural view of an oil reservoir microorganism storage and transportation apparatus according to example 2 of the present invention;
FIG. 4 is a graph comparing the relative abundance of bacteria at the backdoor level for both pressure-loaded and atmospheric transport in accordance with the practice of the present invention;
FIG. 5 is a graph comparing the relative abundance of methanogens after pressure-loaded transport and atmospheric transport in accordance with the practice of the present invention;
FIG. 6 is a graph showing a comparison of relative abundance of bacteria at class level after high pressure culture and normal pressure culture in accordance with the present invention.
Detailed Description
The following detailed description is provided for the purpose of illustrating the embodiments and the advantageous effects thereof, and is not intended to limit the scope of the present disclosure.
Example 1
The present embodiment provides an oil reservoir microorganism storage and transportation device for oil reservoir microorganism pressure-carrying transportation and high-temperature and high-pressure culture, as shown in fig. 1, including: the pressure control system 1 is used for stably controlling the high-pressure environment of the culture container for a long time and accurately controlling the constant pressure in the sampling process; the temperature control device 2 is used for stably controlling the high-temperature environment of the culture system for a long time and automatically cutting off the heating system according to timely alarm; the reactor system 3 is used for carrying out high-temperature and high-pressure culture on the oil deposit microorganisms for a long time; and the sampling system 4 is used for periodically collecting the oil deposit microbial community and the metabolite thereof.
As shown in fig. 2, the pressure control system 1 includes two pressure pumps 11 (displacement pumps), a pressure measuring device 12 (pressure gauge), and a connecting line; the temperature control device 2 includes an incubator 21 (incubator) with a temperature probe 22; the reactor system 3 comprises five temperature-resistant pressure-resistant containers 31 with floating pistons 311, a first three-way valve 32, a second three-way valve 33 and connecting lines which are connected in parallel. The bottom of the temperature-resistant pressure-resistant container 31 is connected with a displacement pump through a first three-way valve 32, and the top of the temperature-resistant pressure-resistant container is connected with a pressure gauge through a second three-way valve 33; the sampling system 4 includes a metering vessel 41 and a buffer vessel 42 through which the gas is vented after the pressure is released.
Example 2
In the present embodiment, a storage and transportation device for reservoir microorganisms is provided, as shown in fig. 3, a reactor system 3 includes 1 temperature-resistant and pressure-resistant container 31 with a floating piston 311. The other structure is the same as embodiment 1.
Example 3
The embodiment takes the apparatus of embodiment 2 as an example to illustrate a specific application process of the apparatus: sampling is carried out in the oil field, and the sample is transported to an oil extraction process research institute in the oil field immediately after sampling. In a laboratory, firstly, an oil phase and a water phase of the produced fluid (the produced fluid can be naturally layered after being placed, and the oil phase and the water phase are separately stored) are loaded into a 1L temperature-resistant pressure-resistant container 31 according to the actual proportion when the produced fluid is produced, and the temperature-resistant pressure-resistant container 31 is subjected to gas removal and pressurization to reservoir pressure by a constant flow pump under the monitoring of a pressure gauge. And then rapidly transported to a laboratory where long-term culture experiments are conducted. As shown in fig. 3, the temperature-resistant and pressure-resistant container 31 is connected to the displacement pump by a connection line, and the temperature-resistant and pressure-resistant container 31 is placed in the incubator 21. The temperature of the thermostat 21 is slowly raised to the reservoir temperature. In order to avoid the rapid rise of the pressure in the culture container caused by the rapid rise of the temperature, the temperature is gradually raised by increasing the amplitude at the temperature of 2-3 ℃, and the temperature is further raised after the pressure is stabilized each time. Each vessel was always monitored by a pressure gauge, controlled by a Quizix series high pressure precision displacement pump (booster pump 11) to maintain the pressure of the culture vessel constant. When the temperature of the incubator is raised to the oil reservoir temperature, and the temperature (60 ℃) and the pressure (12MPa) are stable for 24 hours, the continuous culture of long-term standing is carried out. Sampling was performed at regular time nodes of culture by a Quizix series high pressure precision displacement pump control and monitoring by a pressure gauge (pressure measuring device 12), and the sampling procedure is shown in FIG. 3. When the displacement pump injects sterile water into one end of the temperature-resistant pressure-resistant container 31, the piston moves, and simultaneously, the oil deposit microorganisms are discharged from the other end of the temperature-resistant pressure-resistant container. The liquid sample is collected through the metering vessel 41 and the gas sample is collected after passing through the buffer vessel 42.
The strain test experimental method comprises the following steps: 200ml of water phase in a high-pressure and normal-pressure sample is taken, produced liquid/water phase solution thalli are collected by a 0.22-micron microporous filter membrane, and the filter membrane is sheared to extract total DNA by using FastDNA SPIN Kit for Soil according to the requirements of the specification. The DNA concentration was checked with TBS-380, the DNA purity with NanoDrop2000, and the integrity of the DNA with 1% agarose gel electrophoresis, and then checked with Sequenzer. Analysis mapping was performed after obtaining information on colony 16S rRNA (as shown in FIGS. 4 to 6).
The results show that high pressure retains some important microflora information, especially that of the archaea; after long-term culture at high temperature and high pressure and at high temperature and normal pressure, genome sequencing comparative analysis shows that community distribution of bacteria and archaea is obviously affected by pressure.
Claims (15)
1. An oil reservoir microorganism storage and transportation device, wherein the device comprises a pressure control system (1), a temperature control system (2), and a reactor system (3); pressure control system (1) includes force (forcing) pump (11) and pressure measurement device (12), temperature control system (2) includes thermostated container (21), reactor system (3) include one or more parallelly connected temperature resistant pressure vessel (31) that have floating piston, temperature resistant pressure vessel (31) bottom sets up first three-way valve (32), and the top sets up second three-way valve (33), temperature resistant pressure vessel (31) can be connected with force (forcing) pump (11) through first three-way valve (32) and via the pipeline to can be connected with pressure measurement device (12) respectively through second three-way valve (33) and via the pipeline.
2. The device according to claim 1, wherein the pressurizing pump (11) is a displacement pump.
3. Device according to claim 1 or 2, wherein said incubator (21) further comprises a temperature probe (22) and a temperature alarm device (23).
4. The device according to any one of claims 1 to 3, wherein the pressure pump (11) has a maximum pressure of 69.6MPa and a maximum flow rate of 15 ml/min.
5. The device according to any one of claims 1 to 4, wherein the pressure measuring device (12) is a pressure gauge, and the measuring range of the pressure gauge is 2.0-2.5 times of the experimental pressure.
6. The device according to any one of claims 1 to 5, wherein the maximum pressure resistance and the maximum temperature resistance of the pipeline between the temperature-resistant and pressure-resistant container (31), the first three-way valve (32), the second three-way valve (33), the pressure pump (11), the first three-way valve (32), the second three-way valve (33), the pressure measuring device (12) and the temperature-resistant and pressure-resistant container (31) are respectively 70MPa, and the maximum temperature resistance is respectively 200 ℃.
7. The device according to any one of claims 1 to 6, wherein, in the storage and transportation state, the temperature and pressure resistant container (31) is connected with the pressurizing pump (11) through a first three-way valve (32) at the bottom and is connected with the pressure measuring device (12) through a pipeline, and the temperature and pressure resistant container (31) is arranged in the incubator (21) through a second three-way valve (33) at the top.
8. The device according to any one of claims 1 to 7, wherein the device further comprises a sampling system (4); the sampling system (4) comprises a metering container (41) and a buffer container (42); the top of the temperature-resistant pressure-resistant container (31) can be respectively connected with the pressure measuring device (12) and the metering container (41) through a second three-way valve (33) and a pipeline.
9. The device according to claim 8, wherein the sampling system (4) further comprises a gas collection device (43) connected to the buffer vessel (42) by a pipe.
10. The device according to claim 8 or 9, wherein, in the sampling state, the temperature-resistant and pressure-resistant container (31) is connected with the pressurizing pump (11) through a first three-way valve (32) at the bottom and a pipeline, the top is connected with the pressure measuring device (12) and the metering container (41) through a second three-way valve (33) and a pipeline respectively, and the metering container (41) is connected with the buffer container (42) through a pipeline.
11. A method for preserving oil reservoir microorganisms, wherein the method comprises the following steps:
sampling; and
storage and transportation steps: the use of the device of any of claims 1-10 for storage and transportation of collected microbial samples.
12. The method of claim 11, wherein the warehousing step comprises:
placing the produced liquid containing the microorganism sample obtained in the sampling step into a temperature-resistant pressure-resistant container (31);
discharging gas in the temperature-resistant pressure-resistant container (31), pressurizing the gas to the pressure of an oil reservoir where produced liquid is located, and then closing the first three-way valve (32) and the second three-way valve (33);
placing the temperature-resistant pressure-resistant container (31) in a thermostat (21), and connecting the temperature-resistant pressure-resistant container with a pressure pump (11) through a first three-way valve (32) and via a pipeline, and connecting the temperature-resistant pressure-resistant container with a pressure measuring device (12) through a second three-way valve (33) and via a pipeline, so as to control the pressure in the temperature-resistant pressure-resistant container (31) by using the pressure pump (11); and
and raising the temperature of the constant temperature box (21) to the reservoir temperature.
13. The method according to claim 12, wherein the step of raising the temperature of the thermostat (21) to the reservoir temperature comprises a step-wise raising the temperature with an increase of 4-6 ℃/h, each time after the pressure has stabilized, with a further raising of the temperature.
14. The method of any one of claims 11 to 13, further comprising the step of sampling with any one of 8 to 10 of the reservoir microorganism storage and transportation device.
15. The method of claim 14, wherein the step of sampling comprises driving a floating piston of a temperature and pressure resistant vessel (31) with a booster pump (11) to move to inject production fluid into a metering vessel (41).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010960484.XA CN112266861A (en) | 2020-09-14 | 2020-09-14 | Oil reservoir microorganism storage and transportation device and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010960484.XA CN112266861A (en) | 2020-09-14 | 2020-09-14 | Oil reservoir microorganism storage and transportation device and application |
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| CN112266861A true CN112266861A (en) | 2021-01-26 |
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Application publication date: 20210126 |