CN112263643A

CN112263643A - Pulse activating beverage without sucrose and preservative and preparation method thereof

Info

Publication number: CN112263643A
Application number: CN202011349447.1A
Authority: CN
Inventors: 蔡林凯; 黄珠成; 谢良兵; 蒋娅
Original assignee: Taiji Group Sichuan Tiancheng Pharmaceutical Co ltd
Current assignee: Taiji Group Sichuan Tiancheng Pharmaceutical Co ltd
Priority date: 2020-11-26
Filing date: 2020-11-26
Publication date: 2021-01-26

Abstract

The invention relates to a pulse-activating decoction without sucrose and preservatives and a preparation method thereof, belonging to the technical field of preparation of traditional Chinese medicine preparations. According to the invention, the red ginseng, the radix ophiopogonis, the schisandra chinensis and the steviosin are added into the pulse-activating decoction, the steviosin is used for replacing cane sugar and preservatives in the existing pulse-activating decoction, and the prepared pulse-activating decoction does not influence the use effect, and has the advantages of no preservatives, safer administration, no cane sugar, capability of meeting the use requirements of patients who cannot take cane sugar and the like.

Description

Pulse activating beverage without sucrose and preservative and preparation method thereof

Technical Field

The invention belongs to the technical field of preparation of traditional Chinese medicine preparations, and particularly relates to a pulse-activating decoction without sucrose and preservatives and a preparation method thereof.

Background

The food used today contains more and more preservatives, and the variety of preservatives therein is wide. Since long-term intake of preservatives is not harmful to the human body, and with advances in technology, scientists have increasingly discovered that chemically synthesized preservatives can cause cumulative chronic injuries to the human body, most of which are irreversible. In such cases, the elimination of preservatives is a trend, and many countries review the requirements and relevant standards for the use of chemically synthesized preservatives in food products, and the conditional countries have begun to ban the use of chemically synthesized preservatives in general.

In 2019, 1.16 million adults are estimated to have diabetes in China, and 4.63 million adults are estimated to have diabetes globally. It is expected that the total number of diabetic patients will increase to 5.78 million by 2030 and the total number of uropathic patients will increase to 7 million by 2045 years. Since the dominant diabetic patients cannot eat sugar, and recessive diabetic patients such as those who eat high sugar by mistake can cause serious consequences, the diabetic patients can hardly take sugar-added preparations, and therefore, the reduction and control of sugar intake become one of the matters of great concern. Low-sugar or sugar-free foods (containing medicines) have become the first choice.

The pulse-activating decoction has wide clinical application and definite curative effect, and pharmacological experiments and clinical researches prove that the pulse-activating decoction has the effects of protecting cardiac muscle and improving cardiac function, also has the effects of immunoregulation, scavenging free radicals, promoting growth and development and learning and memory, and is commonly used for treating acute myocardial infarction, coronary heart disease, angina, severe pulmonary heart disease, chronic keshan disease, epidemic hemorrhagic fever, ischemic anemia, infantile intractable spontaneous perspiration, neurosis, toxic myocarditis and other diseases. However, the existing pulse-activating decoction contains sucrose and preservatives, so the use of the pulse-activating decoction in diabetic patients is greatly limited.

Therefore, in order to solve the application limitation caused by the sugar-containing and preservative-containing pulse-activating decoction, the components of the pulse-activating decoction need to be further improved, and a safer and more practical product needs to be prepared.

Disclosure of Invention

In view of the above, an object of the present invention is to provide a pulse-activating decoction without sucrose and preservatives; the invention also aims to provide a preparation method of the pulse-activating decoction which does not contain sucrose and preservatives.

In order to achieve the purpose, the invention provides the following technical scheme:

1. a pulse-activating decoction without sucrose and antiseptic comprises Ginseng radix Rubri, radix Ophiopogonis, fructus Schisandrae chinensis and steviosin.

Preferably, each 1000mL of the pulse-activating decoction comprises 100g of red ginseng, 200g of radix ophiopogonis, 100g of schisandra chinensis, 0.5-1.5 g of steviosin and the balance of water.

Preferably, each 1000mL of the pulse-activating decoction comprises 100g of red ginseng, 200g of radix ophiopogonis, 100g of schisandra chinensis, 0.6-1.0 g of steviosin and the balance of water.

2. The preparation method of the pulse-activating decoction without sucrose and preservatives comprises the following specific steps:

(1) preparing a percolate: pulverizing red ginseng, radix ophiopogonis and schisandra chinensis, adding 65% ethanol, stirring to completely dissolve the materials, soaking for 24 hours, performing percolation treatment, controlling the percolation speed to be 1-3ml per minute per kg of the materials, collecting percolate and concentrating;

(2) and (3) percolating liquid treatment: naturally cooling the percolate obtained by concentrating in the step (1), adding water for diluting, filtering, continuously adding water for diluting, adjusting the pH value to 5-8, adding steviosin, adding water, and stirring to completely dissolve the steviosin;

(3) and (3) post-treatment: and (3) stirring, standing, filtering, filling and sealing the completely dissolved solution in the step (2) and sterilizing to obtain the pulse-activating drink without cane sugar and preservatives.

Preferably, the pulverization in the step (1) is specifically as follows: crushing the red ginseng by using a crusher through a sieve plate with the thickness of 8-12 mm; the fructus Schisandrae chinensis is processed by a universal pulverizer.

Preferably, the percolation treatment in the step (1) is specifically: percolating at a rate of 1-3 ml/kg per minute, and collecting percolate.

Preferably, the pH value is adjusted to be 5.5-7.8 in the step (2).

Preferably, the standing time in the step (3) is not less than 2 h.

Preferably, the sterilization in the step (3) is specifically: the temperature is 100-110 ℃, and the time of the sterilization process is 30-45 min.

The invention has the beneficial effects that:

1. the invention provides a pulse-activating decoction which contains red ginseng, dwarf lilyturf tuber, Chinese magnoliavine fruit and steviosin and does not contain syrup and preservatives, the invention adopts the steviosin to replace the use of cane sugar in the existing pulse-activating decoction, and the application of the preservatives is improved and cancelled by a corresponding preparation method, so that the pulse-activating decoction which is prepared by using natural, safe and stable steviosin to replace cane sugar in the invention perfectly solves the problem that the pulse-activating decoction is limited by the use of the pulse-activating decoction in patients with diabetes in the prior art due to the cane sugar and the preservatives contained in the pulse-activating decoction.

2. The invention also discloses a preparation method of the pulse-activating decoction without sucrose and preservatives, which is characterized in that the pulse-activating decoction without syrup and preservatives, which contains red ginseng, dwarf lilyturf tuber, Chinese magnoliavine fruit and steviosin, is prepared by the modes of preparing percolate, treating the percolate, post-treating and the like, is simple and effective in preparation, and can reduce the sucrose and preservative content on the basis of ensuring the effectiveness of the pulse-activating decoction.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.

Drawings

For the purposes of promoting a better understanding of the objects, aspects and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:

FIG. 1 shows the results of thin layer chromatography with panaxadiol and panaxatriol as reference, wherein a is reference solution I, b as test solution I;

FIG. 2 is a result of thin-layer chromatography with radix Ophiopogonis as control, wherein a is a sample solution II, and b is a control solution II;

FIG. 3 shows the results of thin-layer chromatography with Schisandra chinensis and schizandrol A as reference, wherein a is test solution III, b is reference solution III, and c is reference solution III;

FIG. 4 is a liquid chromatogram I obtained after taking the pulse-activating decoction prepared in example 1 as a test sample;

FIG. 5 is a liquid chromatogram II of the reference solution.

Detailed Description

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It should be noted that, in the following embodiments, features in the embodiments may be combined with each other without conflict.

Example 1

The pulse-activating drink which does not contain sucrose and preservatives is prepared, wherein the specific preparation method comprises the following steps:

(1) preparing a percolate: mixing 200g radix Ophiopogonis, 100g Ginseng radix Rubri (crushed by 10mm sieve plate with crusher) and 100g fructus Schisandrae (processed by universal crusher), adding 65% ethanol, stirring to dissolve completely, soaking for 24 hr, percolating, collecting percolate, and concentrating;

(2) and (3) percolating liquid treatment: naturally cooling the percolate obtained by concentrating in the step (1) (at the speed of 1-3ml per kg of medicinal materials per minute), adding water for diluting, filtering, continuously adding water for diluting, adjusting pH value to 5.5, adding 0.6g of steviosin, adding water, and stirring to completely dissolve;

(3) and (3) post-treatment: and (3) continuously adding water into the solution completely dissolved in the step (2) for regulation, and carrying out stirring, standing for at least 2 hours, filtering, filling and sealing and sterilizing (the sterilization condition is that the temperature is 100 ℃ and the time of the sterilization process is 40min) treatment to obtain 1000ml of the pulse-activating beverage without sucrose and preservatives.

Example 2

(1) preparing a percolate: mixing 200g radix Ophiopogonis, 100g Ginseng radix Rubri (crushed by 8mm sieve plate with crusher) and 100g fructus Schisandrae (processed by universal pulverizer), adding 65% ethanol, stirring to dissolve completely, soaking for 24 hr, percolating, collecting percolate, and concentrating;

(2) and (3) percolating liquid treatment: naturally cooling the percolate obtained by concentrating in the step (1) (at the speed of 1-3ml per kg of medicinal materials per minute), adding water for diluting, filtering, continuously adding water for diluting, adjusting pH to 5, adding 0.5g of steviosin, adding water, and stirring to completely dissolve;

(3) and (3) post-treatment: and (3) continuously adding water into the solution completely dissolved in the step (2), and performing stirring, standing for at least 2h, filtering, filling and sealing, and sterilizing (under the sterilization condition that the temperature is 110 ℃ and the time of the sterilization process is 30min) to obtain 1000ml of the pulse-activating beverage without sucrose and preservatives.

Example 3

(1) preparing a percolate: mixing 200g radix Ophiopogonis, 100g Ginseng radix Rubri (crushed by 12mm sieve plate with crusher) and 100g fructus Schisandrae (processed by universal crusher), adding 65% ethanol, stirring to dissolve completely, soaking for 24 hr, percolating, collecting percolate, and concentrating;

(2) and (3) percolating liquid treatment: naturally cooling the percolate obtained by concentrating in the step (1) (at the speed of 1-3ml per kg of medicinal materials per minute), adding water for diluting, filtering, continuously adding water for diluting, adjusting pH to 8, adding 1.5g of steviosin, adding water, and stirring to completely dissolve;

(3) and (3) post-treatment: and (3) continuously adding water into the solution completely dissolved in the step (2) for regulation, and carrying out stirring, standing for at least 2h, filtering, filling and sealing and sterilizing treatment (the sterilization condition is that the temperature is 100 ℃ and the time of the sterilization process is 45min) to obtain 1000ml of the pulse-activating beverage without cane sugar and preservatives.

Example 4

(2) and (3) percolating liquid treatment: naturally cooling the percolate obtained by concentrating in the step (1) (at the speed of 1-3ml per kg of medicinal materials per minute), adding water for diluting, filtering, continuously adding water for diluting, adjusting pH value to 7.8, adding 1.0g of steviosin, adding water, and stirring to completely dissolve;

Decoction for identifying pulse-activating

The quality standard detection is carried out on the pulse-activating decoction prepared in the example 1, and the specific detection items are as follows:

1. the characteristics are as follows: the pulse-activating decoction prepared in example 1 was tested to have the following properties: the yellowish-brown to reddish-brown clear liquid is fragrant, sweet and sour in taste and slightly bitter, so that the pulse-activating drink prepared in example 1 meets the requirements on properties in the quality standard detection of the pulse-activating drink (yellowish-brown to reddish-brown clear liquid, fragrant, sweet and sour in taste and slightly bitter).

2. And (4) checking: the relative density and the pH of the pulse-activating decoction prepared in the example 1 are detected to be 1.06 and 6.4, so that the pulse-activating decoction prepared in the example 1 meets the requirements on the relative density (not less than 1.04) and the pH (4.5-7.0) in the quality standard detection of the pulse-activating decoction;

3. and (3) identification: the pulse-activating decoction prepared in the example 1 can detect spots corresponding to the panaxadiol and panaxatriol reference substances, spots corresponding to the radix ophiopogonis reference medicinal materials and spots corresponding to the schisandra chinensis reference medicinal materials and the schizandrol A reference substances by thin-layer chromatography detection;

the specific detection method is as follows:

a. carrying out thin-layer chromatography detection by using panaxadiol and panaxatriol as controls:

(1) preparing a test solution I: 20ml of the shengmai beverage prepared in example 1 and containing no sucrose and preservative was taken, extracted with 20ml of n-butanol with shaking, the n-butanol solution was evaporated to dryness, 15ml of a 45% ethanol solution of sulfuric acid (7 → 100) was added to the residue (7 ml of sulfuric acid was taken, and diluted to 100ml with 45% ethanol to obtain a 45% ethanol solution of sulfuric acid), heated and refluxed for 1 hour, ethanol was volatilized, extracted with 10ml of chloroform with shaking, the chloroform fraction was separated, washed with water to neutrality, dehydrated with an appropriate amount of anhydrous sodium sulfate, filtered, and the filtrate was concentrated to 1ml to serve as a test sample solution I.

(2) Preparation of control solution I: and adding anhydrous ethanol into control panaxadiol and panaxatriol to obtain mixed solution containing 1mg of panaxadiol and 1mg of panaxatriol per 1ml, and making into control solution I.

(3) Thin layer chromatography (general 0502) test: sucking 10 μ l of each of the sample solution I prepared in the step (1) and the control solution I prepared in the step (2), respectively dropping the sample solution I and the control solution I on the same silica gel G thin layer plate, developing with cyclohexane-acetone (2:1) mixed solution as a developing agent, taking out, air drying, spraying with a sulfuric acid methanol solution (1 → 2) (i.e. taking 1ml of sulfuric acid, diluting with methanol to 2ml to obtain a sulfuric acid methanol solution), heating at 105 ℃ for about 10 minutes, placing under an ultraviolet lamp with a wavelength of 365nm for inspection, and displaying fluorescent spots of the same color in the chromatogram of the sample solution I at positions corresponding to the chromatogram of the control solution I, as shown in FIG. 1, wherein a is the control solution I, b as the sample solution I. The detection result shows that the pulse-activating decoction meets the specification of the thin-layer chromatography identification of the panaxadiol and the panaxatriol in the quality standard.

b. Performing thin-layer chromatography detection by taking radix ophiopogonis as a control:

(1) preparing a test solution II: taking 10ml of the pulse-activating decoction without sucrose and preservative prepared in example 1, adding 0.5ml of hydrochloric acid and 1ml of water, heating and boiling for 5min, cooling, extracting with 20ml of chloroform with shaking, separating chloroform solution, and concentrating to 1ml to obtain a test solution II.

(2) Preparing a reference medicinal material solution II: decocting 1g radix Ophiopogonis as control with 20ml water for 10min, filtering, adding 0.5ml hydrochloric acid into the filtrate, and making into control solution II by the same method (boiling for 5min, cooling, extracting with chloroform 20ml under shaking, separating chloroform solution, and concentrating to 1 ml).

(3) Thin layer chromatography (general 0502) test: sucking 5 μ l of each of the test solution II and the control solution II, respectively dropping on the same silica gel G thin layer plate, developing with mixed solution of chloroform and acetone at a volume ratio of 4:1 as developing agent, taking out, air drying, spraying 10% sulfuric acid ethanol solution (80ml ethanol solution slowly adding 10ml sulfuric acid, cooling, adding ethanol solution to 100ml to obtain 10% sulfuric acid ethanol solution), and heating at 100 deg.C until the spots are clearly developed. The detection result shows that the main spots with the same color appear in the chromatogram of the test solution II and at the position corresponding to the chromatogram of the reference medicinal material solution II, as shown in FIG. 2, wherein a is the test solution II and b is the reference medicinal material solution II. The detection result shows that the pulse-activating decoction meets the thin-layer chromatography identification regulation of the radix ophiopogonis in the quality standard.

c. Performing thin-layer chromatography detection by using schisandra chinensis and schizandrol A as controls:

(1) preparing a test solution III: 10ml of the pulse-activating decoction prepared in example 1 and containing no sucrose and preservative was taken, 20ml of water was added, shaking was carried out, extraction was carried out with shaking of 30ml of ether, repetition was carried out for 3 times, the ether solution was combined and evaporated to dryness, and 1ml of ethanol was added to the residue to dissolve it, thereby obtaining a sample solution III.

(2) Preparing a schisandra chinensis control solution III: adding 20ml chloroform into 1g fructus Schisandrae chinensis control medicinal material, ultrasonic treating for 30min, filtering, evaporating filtrate, and dissolving the residue with 1ml ethanol to obtain control medicinal material solution III.

(3) Preparing a schizandrol A reference substance solution III: and adding chloroform into the schizandrol A control to obtain a solution containing 1mg of schizandrol A per 1ml, and using as the schizandrol A control solution III.

(4) Thin layer chromatography (general 0502) test: sucking 5-10 mul of the test solution prepared in the step (1)III, 2-5 mul of schisandra chinensis contrast solution III and 2-5 mul of schizandrol A contrast solution III are respectively spotted on the same silica gel GF₂₅₄And (3) taking the upper solution of petroleum ether (30-60 ℃) and ethyl formate-formic acid (15:5:1) as a developing agent on the thin-layer plate, developing, taking out, airing, and placing under an ultraviolet lamp with the wavelength of 254nm for inspection. In the chromatogram of the test solution, spots of the same color appear at the positions corresponding to the chromatograms of the Schisandra chinensis control solution and the schizandrol A control solution, as shown in FIG. 3, wherein a is test solution III, b is control solution III, and c is control solution III. The detection result shows that the pulse-activating decoction meets the thin-layer chromatography identification regulation of the schisandra chinensis and the schizandrol A in the quality standard.

4. The microbial limit: the number of bacteria of the pulse-activating decoction prepared in example 1 was measured, and the results were: total aerobic bacteria count < 10cfu/ml, total mold and yeast count < 10cfu/ml, no detected Escherichia coli (1 ml); it is shown that the pulse-activating decoction prepared in example 1 satisfies the requirement of oral liquid for microbial limitation (total aerobic bacteria number is not more than 10)²cfu/ml, total number of mould and yeast not more than 10¹cfu/ml, Escherichia coli (1ml) could not be detected.

5. Content determination: the shengmai beverage prepared in example 1 is subjected to content detection of schizandrol A, the detection result is 1.36 mg/branch, and the requirement that the content of the schizandrol A in the quality standard is not less than 0.75 mg/branch is met.

6. Characteristic spectrum:

(1) preparing a test solution: the pulse-activating decoction prepared in example 1 was filtered through a 0.45 μm microporous membrane, and the subsequent filtrate was collected to obtain a test solution.

(2) Preparation of reference solutions: taking ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rb2, ginsenoside Rd, schisandrin A and schisantherin A as reference substances, respectively, preparing reference substance solution (firstly taking schisandrin A and schisantherin A, precisely weighing, adding methanol to dissolve completely, fixing volume with methanol to form stock solution, then taking ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rb2 and ginsenoside Rd, precisely weighing, adding methanol to dissolve completely, adding precisely weighed stock solution, and adding methanol to fix volume) to obtain concentrations of 0.4g/L, 0.08g/L, and, 0.08g/L of the reference solution.

(3) Performing liquid chromatography detection on the test solution and the reference solution under the same conditions and in the same operation process to obtain a liquid chromatogram I (shown in FIG. 4) of the test solution and a liquid chromatogram II (shown in FIG. 5) of the reference solution, wherein the specific process of the liquid chromatography detection is as follows: precisely sucking 10 μ L of reference solution and 10 μ L of test solution, respectively, and injecting into liquid chromatograph for liquid chromatography detection;

wherein the detection process of the liquid chromatography is carried out according to the following conditions: a chromatographic column: waters SymmetryShield^TMPerforming gradient elution with RP18(4.6mm × 250mm × 5.0 μm), column temperature of 30 deg.C, flow rate of 1.0ml/min, acetonitrile as mobile phase A, and water as mobile phase B, detecting wavelength of 203nm, and theoretical plate number calculated according to ginsenoside Rb1 peak should not be lower than 40000;

the gradient of mobile phase a and mobile phase B when eluted in gradient is shown in table 1 below:

TABLE 1 composition of mobile phase A and mobile phase B at gradient elution

"→" indicates a gradual increase.

(4) The retention times of ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rb2, ginsenoside Rd, schisandrin and schisantherin in the liquid chromatography detection process were confirmed by the above fig. 5, as shown in table 2 below.

(5) The quality standard of the pulse-activating decoction prepared in example 1 is shown to be satisfied by comparing the retention time of the characteristic peak appearing in the liquid chromatogram I (FIG. 4) with the retention time of the characteristic peak of each reference substance in FIG. 5 (the liquid chromatogram I shows 9 characteristic peaks with the same retention time of the chromatographic peaks of the 9 reference substances in the liquid chromatogram II).

TABLE 2 retention time of different references in liquid chromatogram

Similarly, the pulse-activating decoction prepared in examples 2 to 4 was tested, and similarly, the corresponding characteristic peaks of 9 reference substances could be found in the same retention time in the corresponding liquid chromatogram obtained in examples 2 to 4, which indicates that the pulse-activating decoction prepared in examples 2 to 4 also meets the quality testing standard of the pulse-activating decoction.

In conclusion, the invention provides the pulse-activating decoction which contains red ginseng, dwarf lilyturf tuber, Chinese magnoliavine fruit and steviosin and does not contain syrup and preservatives, the invention adopts the steviosin to replace the use of cane sugar in the existing pulse-activating decoction, and improves and cancels the application of the preservatives through the corresponding preparation method, so that the pulse-activating decoction which is prepared by using natural, safe and stable steviosin to replace cane sugar in the invention perfectly solves the problem that the pulse-activating decoction in the prior art is limited by the use of the pulse-activating decoction in patients with diabetes due to the cane sugar and the preservatives contained in the pulse-activating decoction. In addition, the invention also discloses a preparation method of the pulse-activating decoction without sucrose and preservatives, which is characterized in that the pulse-activating decoction containing red ginseng, dwarf lilyturf tuber, Chinese magnoliavine fruit and steviosin and not containing syrup and preservatives is prepared by the modes of preparing percolate, treating the percolate, post-treating and the like, the preparation method is simple and effective, and the contents of sucrose and preservatives in the pulse-activating decoction can be reduced on the basis of ensuring the effectiveness of the pulse-activating decoction.

Finally, the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the claims of the present invention.

Claims

1. A pulse-activating drink which does not contain sucrose and preservatives is characterized by comprising red ginseng, dwarf lilyturf tuber, Chinese magnoliavine fruit and stevioside.

2. The pulse-activating decoction according to claim 1, wherein each 1000mL of the pulse-activating decoction comprises 100g of red ginseng, 200g of radix ophiopogonis, 100g of schisandra chinensis, 0.5-1.5 g of steviosin and the balance of water.

3. The pulse-activating decoction according to claim 1, wherein each 1000mL of the pulse-activating decoction comprises 100g of red ginseng, 200g of radix ophiopogonis, 100g of schisandra chinensis, 0.6-1.0 g of stevioside and the balance of water.

4. The method for preparing the pulse-activating decoction without sucrose and preservatives as described in any one of claims 1 to 3, wherein the method comprises the following steps:

5. The preparation method according to claim 4, wherein the pulverization in the step (1) is specifically: crushing the red ginseng by using a crusher through a sieve plate with the thickness of 8-12 mm; the fructus Schisandrae chinensis is processed by a universal pulverizer.

6. The preparation method according to claim 4, wherein the percolation treatment in the step (1) is specifically: percolating at a rate of 1-3 ml/kg per minute, and collecting percolate.

7. The method according to claim 4, wherein the pH value is adjusted to 5.5 to 7.8 in the step (2).

8. The method according to claim 4, wherein the standing time in the step (3) is not less than 2 hours.

9. The method according to claim 4, wherein the sterilization in step (3) is specifically: the temperature is 100-110 ℃, and the time of the sterilization process is 30-45 min.