CN112222121A - Cleaning device and immunoassay appearance of reaction tube - Google Patents
Cleaning device and immunoassay appearance of reaction tube Download PDFInfo
- Publication number
- CN112222121A CN112222121A CN202011125160.0A CN202011125160A CN112222121A CN 112222121 A CN112222121 A CN 112222121A CN 202011125160 A CN202011125160 A CN 202011125160A CN 112222121 A CN112222121 A CN 112222121A
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- China
- Prior art keywords
- waste liquid
- flow
- liquid
- reaction tube
- controller
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
- B08B9/0813—Cleaning containers having tubular shape, e.g. casks, barrels, drums by the force of jets or sprays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
Abstract
The invention relates to a cleaning device of a reaction tube, which is used for cleaning the reaction tube in an immunoassay analyzer and comprises a liquid injection component and a liquid suction component; the liquid injection assembly comprises a liquid injection piece; the liquid suction assembly comprises a liquid suction piece, a waste liquid pipe and an anti-blocking system; wherein, prevent that the jam system includes first flow sensor, second flow sensor and controller, first flow sensor is used for measuring the first waste liquid flow of waste liquid pipe and imbibition spare junction, second flow sensor is used for measuring the second waste liquid flow of waste liquid pipe one end of keeping away from imbibition spare, first flow sensor, the equal controller electricity of second flow sensor is connected, the controller calculates according to first waste liquid flow and second waste liquid flow, and judge whether the waste liquid pipe blocks up according to the calculated result, it can in time know the jam condition of waste liquid pipe, the analysis staff of being convenient for in time dredges the waste liquid pipe, the waste liquid pipe has been prevented to take place to block up.
Description
Technical Field
The invention relates to the technical field of chemical immunoassay, in particular to a cleaning device for a reaction tube and an immunoassay analyzer with the same.
Background
Luminescence immunoassay is based on immunological reaction of antigen-antibody combination, uses enzyme, luminescent agent and other substances to mark antigen-antibody, and analyzes the content of analyte in sample by linking light signal with analyte concentration through luminescence reaction.
In the cleaning process, firstly, magnetic particles are used as a solid phase carrier to capture and combine target analytes in a sample, then the magnetic particles directly or indirectly combined with the target analytes are collected on the inner side wall of a reaction tube through magnetic force, and after a cleaning solution is injected and waste liquid is extracted for multiple times, free markers and other interference impurities which are not combined on the magnetic particles are finally removed so as to measure signals of antigen-antibody conjugates connected on the magnetic particles.
To traditional cleaning method, at the extraction waste liquid in-process, because the heterogeneous impurity of solid-liquid content is more in the waste liquid, therefore can cause when using for a long time and inhale waste liquid pipeline jam, if not dredge in time can cause the waste liquid to absorb incompletely, and then lead to the reaction tube to wash unclean to influence subsequent analysis experimental result, do not have at present and can effectively judge and inhale the technique that whether waste liquid pipeline blockked up, therefore can not be timely to inhale waste liquid pipeline and dredge.
Disclosure of Invention
In view of this, it is necessary to provide a cleaning device for a reaction tube, so as to solve the technical problem in the prior art that the blockage of the waste liquid suction pipeline cannot be found in time.
The invention provides a cleaning device of a reaction tube, which is used for cleaning the reaction tube in an immunoassay analyzer and comprises a liquid injection component and a liquid suction component;
the liquid injection assembly comprises a liquid injection part for injecting cleaning liquid into the reaction tube;
the liquid suction assembly comprises a liquid suction piece for sucking waste liquid from the reaction tube, a waste liquid tube connected with the liquid suction piece and used for discharging the waste liquid out of the immunoassay analyzer, and an anti-blocking system for preventing the waste liquid tube from being blocked;
the anti-blocking system comprises a first flow sensor, a second flow sensor and a controller, wherein the first flow sensor is used for measuring the first waste liquid flow at the joint of the waste liquid pipe and the liquid absorbing piece, the second flow sensor is used for measuring the second waste liquid flow at one end, far away from the liquid absorbing piece, of the waste liquid pipe, the first flow sensor and the second flow sensor are electrically connected with the controller, and the controller calculates according to the first waste liquid flow and the second waste liquid flow and judges whether the waste liquid pipe is blocked or not according to the calculation result.
Further, the controller comprises an acquisition module, a pre-input module, a calculation module and a judgment module; the acquisition module is used for acquiring the first waste liquid flow and the second waste liquid flow, the pre-input module is used for inputting and storing a preset flow difference threshold value, the calculation module is used for calculating an actual flow difference value of the first waste liquid flow and the second waste liquid flow, and the judgment module is used for judging the size relation between the preset flow difference threshold value and the actual flow difference value.
Further, the anti-blocking system further comprises a timer, the timer is electrically connected with the controller, and the controller periodically obtains the first waste liquid flow and the second waste liquid flow according to the time preset by the timer.
Furthermore, the anti-blocking system further comprises an alarm, the controller further comprises a sending module, the alarm is electrically connected with the controller, and the sending module is used for sending an alarm instruction to the alarm.
Further, the alarm is a buzzer and/or a warning lamp.
Further, imbibition subassembly still includes fixed plate, support and actuating mechanism, actuating mechanism install in the fixed plate and with the leg joint, in order to drive the support up-and-down motion, imbibition piece install in the support, in order to stretch out or withdraw from under the drive of support the reaction tube.
Further, the support comprises a supporting plate, a bottom plate and a top plate, the top plate is arranged above the reaction tube, the bottom plate is arranged close to the fixed plate horizontally, the upper end of the supporting plate is connected with the top plate, the lower end of the supporting plate is connected with the bottom plate, the driving mechanism comprises a lead screw motor and a guide rod, the guide rod is vertically fixed on the fixed plate, the bottom plate is movably sleeved on the upper end of the guide rod, a lead screw shaft of the lead screw motor is in threaded connection with the bottom plate, an avoiding groove is formed in the supporting plate corresponding to the lead screw shaft, and the liquid absorbing part is installed on the top plate.
Further, the liquid absorbing piece is an elongated liquid absorbing needle.
Further, annotate the liquid piece including annotating the liquid pipe and annotating the liquid needle, the roof both sides extend and are formed with the support arm, annotate the liquid needle install in the support arm.
The application also provides an immunoassay analyzer, which comprises a rotary disc and the cleaning device of the reaction tube, wherein a plurality of reactor placing holes are formed on the rotary disc at intervals along the circumferential direction of the rotary disc, and the rotary disc is transversely and rotatably arranged on the machine body.
According to the cleaning device for the reaction tube, the first flow sensor is used for measuring the first waste liquid flow at the joint of the waste liquid tube and the liquid absorbing piece, the second flow sensor is used for measuring the second waste liquid flow at the end, far away from the liquid absorbing piece, of the waste liquid tube, and then the controller is used for calculating and judging whether the waste liquid tube is blocked or not, so that the blocking condition of the waste liquid tube can be known in time, an analysis worker can conveniently dredge the waste liquid tube in time, and the waste liquid tube is prevented from being blocked.
The foregoing is a summary of the present invention, and in order to provide a clear understanding of the technical means of the present invention and to be implemented in accordance with the present specification, the following is a detailed description of the preferred embodiments of the present invention.
Drawings
Fig. 1 is a schematic structural diagram of a cleaning apparatus for a reaction tube according to an embodiment of the present invention;
FIG. 2 is a schematic logical structure diagram of an anti-jamming system according to a first embodiment of the present invention;
FIG. 3 is a schematic structural diagram of another view of a cleaning apparatus for a reaction tube according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of an immunoassay analyzer according to a second embodiment of the present invention.
Detailed Description
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate preferred embodiments of the invention and together with the description, serve to explain the principles of the invention and not to limit the scope of the invention.
Example one
As shown in fig. 1 and 2, the present application discloses a reaction tube cleaning device for cleaning a reaction tube a in an immunoassay analyzer, comprising a liquid injection assembly 10 and a liquid suction assembly 20;
the liquid injection assembly 10 comprises a liquid injection part for injecting cleaning liquid into the reaction tube;
the liquid injection member 1 comprises a liquid injection pipe 12 and a liquid injection needle 11, wherein two sides of the top plate 253 extend to form a supporting arm 254, the liquid injection needle 11 is installed on the supporting arm 254, cleaning liquid is injected into the reaction pipe through the liquid injection pipe 12 and the liquid injection needle, and a power system for liquid injection is generally arranged in the cleaning liquid storage device.
The liquid suction assembly 20 includes a liquid suction member 21 for sucking waste liquid from the reaction tube, a waste liquid tube 22 connected to the liquid suction member 21 for discharging waste liquid outside the immunoassay analyzer, and an anti-clogging system 23 for preventing clogging of the waste liquid tube, and it should be noted that the cleaning device for reaction tubes disclosed in the present application is a soft and hard combined device, and the anti-clogging system 23 is generally built inside the immunoassay analyzer in the use scene thereof.
As shown in fig. 2, the anti-clogging system 23 includes a first flow sensor 231, a second flow sensor 232, and a controller 233, wherein the first flow sensor 231 is used for measuring a first waste liquid flow Q1 at a connection position of the waste liquid pipe 22 and the liquid suction member 21, the second flow sensor 232 is used for measuring a second waste liquid flow Q2 at an end of the waste liquid pipe 22 away from the liquid suction member 21, both the first flow sensor 231 and the second flow sensor 232 are electrically connected to the controller 233, and the controller 233 performs calculation according to the first waste liquid flow Q1 and the second waste liquid flow Q2, and determines whether the waste liquid pipe 22 is clogged according to the calculation result.
Specifically, in some embodiments of the present application, the controller 233 includes an obtaining module 2331, a pre-inputting module 2332, a calculating module 2333, and a determining module 2334; the obtaining module 2331 is configured to obtain the first waste liquid flow Q1 and the second waste liquid flow Q2, the pre-inputting module 2332 is configured to input and store a preset flow difference threshold Δ Q, the calculating module 2333 is configured to calculate actual flow difference values Q1-Q2 of the first waste liquid flow Q1 and the second waste liquid flow Q2, and the judging module 2334 is configured to judge a size relationship between the preset flow difference threshold Δ Q and the actual flow difference values Q1-Q2.
It can be understood that the preset flow difference threshold value Δ Q is determined by a previous experiment, and is related to the material, length and thickness of the waste liquid pipe 22, and for a section of the waste liquid pipe 22, when the flow rate of the liquid entering from the front end and the flow rate of the liquid output from the rear end are greater than the preset flow difference threshold value Δ Q, it indicates that the flow difference between the front end and the rear end of the waste liquid pipe is large, and the waste liquid pipe is blocked and needs to be dredged in time; to immunoassay appearance's waste liquid pipe mediation, generally adopt the mode that the running water washed, in the clear water that the impurity degree is less than certain numerical value inputs the waste liquid intraductal through imbibition piece promptly, can effectually block up impurity discharge in the waste liquid pipe under water pressure reaches certain circumstances.
In some embodiments, the controller 233 can be directly integrated into the control system of the immunoassay analyzer, which can effectively reduce the size of the immunoassay analyzer and facilitate the overall control.
In some embodiments of the present application, the anti-clogging system 23 further comprises a timer 234, the timer 234 is electrically connected to the controller 233, and the controller 233 periodically obtains the first waste liquid flow rate Q1 and the second waste liquid flow rate Q2 according to a time preset by the timer 234.
In practical use, in the waste liquid suction process, the time period for obtaining the first waste liquid flow Q1 and the second waste liquid flow Q2 is generally not more than 2h, so that the blockage prevention system 23 can be effectively ensured to timely and effectively prevent the blockage of the waste liquid pipe 22.
The anti-blocking system 23 further comprises an alarm 235, the controller 233 further comprises a sending module 2335, the alarm 235 is electrically connected to the controller 233, and the sending module 2335 is configured to send an alarm instruction to the alarm 235.
Generally, when the determination module 2334 determines Q1-Q2 > Δ Q, the sending module 2336 sends an alarm instruction to the alarm 235 to make the alarm 235 alarm to prompt the staff to perform the work of dredging.
In some embodiments, the alarm 235 is a buzzer and/or a warning light.
As shown in fig. 1, as a preferred embodiment, the pipetting assembly 20 further comprises a fixing plate 24, a bracket 25 and a driving mechanism 26, wherein the driving mechanism 26 is mounted on the fixing plate 24 and connected with the bracket 25 to drive the bracket 25 to move up and down, and the pipetting member 21 is mounted on the bracket 25 to extend out of or withdraw from the reaction tube a under the driving of the bracket 25.
With continued reference to fig. 1 and 3, the support 25 includes a support plate 251, a bottom plate 252 and a top plate 253, the top plate 253 is disposed above the reaction tube a, the bottom plate 252 is disposed horizontally adjacent to the fixing plate 24, the upper end of the support plate 251 is connected to the top plate 253, the lower end of the support plate 251 is connected to the bottom plate 252, the driving mechanism 26 includes a lead screw motor 261 and a guide rod 262, the guide rod 262 is vertically fixed to the fixing plate 24, the bottom plate 252 is movably sleeved on the upper end of the guide rod 262, the lead screw motor 261 is fixedly mounted at the lower end of the fixing plate 24, a lead screw shaft of the lead screw motor 261 penetrates through the fixing plate 24 and is in threaded connection with the bottom plate 252, an avoiding groove 251a is formed on the support plate 251 corresponding to the lead screw shaft, and the liquid.
In the embodiment of the present application, the liquid absorbing member 21 is preferably an elongated liquid absorbing needle, and it is understood that the number of the liquid absorbing needles may be determined according to actual conditions, and in order to ensure that the waste liquid is completely absorbed, in some embodiments of the present application, the number of the liquid absorbing needles is two, and the two liquid absorbing needles are arranged in parallel at the lower end of the top plate 253.
In the embodiment of the application, when the screw rod motor 261 is controlled by the immunoassay analyzer to operate, the bracket 25 is driven to move downwards, the liquid injection needle 11 is driven by the bracket 25 to extend into the reaction tube a, the cleaning liquid is injected, then the bracket 25 is lifted, the liquid injection needle 11 is driven by the bracket 25 to separate from the reaction tube a, the reaction tube a moves to correspond to the liquid suction needle, the bracket 25 moves downwards again, the liquid suction needle sucks the waste liquid, at this time, under the preset time regulation of the timer 234, the first flow sensor 231 measures the first waste liquid flow Q1 at the connection position of the waste liquid tube 22 and the liquid suction member 21, the second flow sensor 232 measures the second waste liquid flow Q2 at the end of the waste liquid tube 22 far away from the liquid suction member 22, the obtaining module 2331 obtains the first waste liquid flow Q1 and the second waste liquid flow Q2, the operator inputs and stores the preset flow difference threshold Q3875-38764 at the pre-input module 2332, the calculating module 2333 calculates the actual difference Q1 and the actual flow Q3875-38764 of the second waste liquid flow Q2, the judging module 2334 judges the magnitude relation between the preset flow difference threshold value delta Q and the actual flow difference value Q1-Q2, if Q1-Q2 is less than delta Q, the waste liquid pipe 22 is not blocked and is not processed, if Q1-Q2 is less than delta Q, the waste liquid pipe 22 is blocked, and the sending module 2335 sends an alarm instruction to the alarm 235 to enable the alarm 235 to alarm to prompt a worker to dredge.
Example two
As shown in fig. 4, the present application further provides an immunoassay analyzer, which comprises a body 31, a turntable 32 and a device for cleaning reaction tubes as described in the first embodiment, wherein the turntable 30 is formed with a plurality of reactor placing holes 32a at intervals along its circumferential direction, the turntable 32 is transversely rotatably mounted on the body 31, the reactor placing holes 32a serve as placing positions for the reaction tubes a, and the reaction tubes a are rotated with the turntable to realize position change.
The immunoassay appearance that this embodiment provided, the first waste liquid flow of waste liquid pipe and imbibition piece junction is measured through first flow sensor, the second waste liquid flow of the one end of imbibition piece is kept away from to the waste liquid pipe through second flow sensor measurement, then calculate and judge whether the waste liquid pipe blocks up through the controller, can in time know the jam condition of waste liquid pipe, the analysis staff of being convenient for in time dredges the waste liquid pipe, it blocks up to have prevented that the waste liquid pipe from taking place, concrete implementation is no longer repeated here, can specifically look up embodiment one.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (10)
1. A cleaning device of a reaction tube is used for cleaning the reaction tube in an immunoassay analyzer and is characterized by comprising a liquid injection component and a liquid suction component;
the liquid injection assembly comprises a liquid injection part for injecting cleaning liquid into the reaction tube;
the liquid suction assembly comprises a liquid suction piece for sucking waste liquid from the reaction tube, a waste liquid tube connected with the liquid suction piece and used for discharging the waste liquid out of the immunoassay analyzer, and an anti-blocking system for preventing the waste liquid tube from being blocked;
the anti-blocking system comprises a first flow sensor, a second flow sensor and a controller, wherein the first flow sensor is used for measuring the first waste liquid flow at the joint of the waste liquid pipe and the liquid absorbing piece, the second flow sensor is used for measuring the second waste liquid flow at one end, far away from the liquid absorbing piece, of the waste liquid pipe, the first flow sensor and the second flow sensor are electrically connected with the controller, and the controller calculates according to the first waste liquid flow and the second waste liquid flow and judges whether the waste liquid pipe is blocked or not according to the calculation result.
2. The cleaning device for the reaction tube according to claim 1, wherein the controller comprises an acquisition module, a pre-input module, a calculation module and a judgment module; the acquisition module is used for acquiring the first waste liquid flow and the second waste liquid flow, the pre-input module is used for inputting and storing a preset flow difference threshold value, the calculation module is used for calculating an actual flow difference value of the first waste liquid flow and the second waste liquid flow, and the judgment module is used for judging the size relation between the preset flow difference threshold value and the actual flow difference value.
3. The apparatus for cleaning a reaction tube according to claim 2, wherein the anti-clogging system further comprises a timer electrically connected to the controller, and the controller periodically obtains the first waste liquid flow rate and the second waste liquid flow rate according to a time preset by the timer.
4. The cleaning device for the reaction tube according to claim 2, wherein the anti-clogging system further comprises an alarm, the controller further comprises a sending module, the alarm is electrically connected with the controller, and the sending module is used for sending an alarm instruction to the alarm.
5. The cleaning device for reaction tubes according to claim 4, wherein the alarm is a buzzer and/or a warning light.
6. The apparatus for cleaning reaction tubes according to claim 1, wherein the pipetting assembly further comprises a fixing plate, a holder and a driving mechanism, the driving mechanism is mounted on the fixing plate and connected to the holder to drive the holder to move up and down, and the pipetting member is mounted on the holder to extend out of or withdraw from the reaction tubes under the driving of the holder.
7. The apparatus for cleaning reaction tubes according to claim 6, wherein the holder comprises a support plate, a bottom plate and a top plate, the top plate is disposed above the reaction tubes, the bottom plate is horizontally disposed adjacent to the fixing plate, the support plate has an upper end connected to the top plate and a lower end connected to the bottom plate, the driving mechanism comprises a screw motor and a guide rod, the guide rod is vertically fixed to the fixing plate, the bottom plate is movably sleeved on the upper end of the guide rod, the screw motor is fixedly mounted on the lower end of the fixing plate, a screw shaft of the screw motor penetrates through the fixing plate and is in threaded connection with the bottom plate, the support plate is formed with an avoidance groove corresponding to the screw shaft, and the liquid absorbing member is mounted on the top plate.
8. The apparatus for washing reaction tubes according to claim 7, wherein the liquid-absorbing member is an elongated liquid-absorbing needle.
9. The apparatus for cleaning reaction tubes according to claim 7, wherein the liquid injection member includes a liquid injection tube and a liquid injection needle, support arms are formed on both sides of the top plate in an extending manner, and the liquid injection needle is mounted on the support arms.
10. An immunoassay analyzer comprising a body, a rotary plate having a plurality of reactor holding holes formed at intervals along a circumferential direction thereof, and a cleaning device for the reaction tube according to any one of claims 1 to 9, the rotary plate being transversely rotatably mounted to the body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011125160.0A CN112222121A (en) | 2020-10-20 | 2020-10-20 | Cleaning device and immunoassay appearance of reaction tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011125160.0A CN112222121A (en) | 2020-10-20 | 2020-10-20 | Cleaning device and immunoassay appearance of reaction tube |
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| Publication Number | Publication Date |
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| CN112222121A true CN112222121A (en) | 2021-01-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011125160.0A Pending CN112222121A (en) | 2020-10-20 | 2020-10-20 | Cleaning device and immunoassay appearance of reaction tube |
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| CN (1) | CN112222121A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113578905A (en) * | 2021-07-15 | 2021-11-02 | 深圳市爱康生物科技有限公司 | Plate washing machine needle blockage detection device and method |
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Application publication date: 20210115 |