CN112167124A - Method for improving hatching rate of mandarin fish fertilized eggs and uniformity of seeds - Google Patents
Method for improving hatching rate of mandarin fish fertilized eggs and uniformity of seeds Download PDFInfo
- Publication number
- CN112167124A CN112167124A CN202011150618.8A CN202011150618A CN112167124A CN 112167124 A CN112167124 A CN 112167124A CN 202011150618 A CN202011150618 A CN 202011150618A CN 112167124 A CN112167124 A CN 112167124A
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- China
- Prior art keywords
- mandarin fish
- rate
- uniformity
- hatching
- improving
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Links
- 241000404975 Synchiropus splendidus Species 0.000 title claims abstract description 38
- 230000012447 hatching Effects 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 15
- 235000013601 eggs Nutrition 0.000 title claims description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000012528 membrane Substances 0.000 claims description 22
- 238000001125 extrusion Methods 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000004075 alteration Effects 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000004720 fertilization Effects 0.000 abstract description 3
- 241000251468 Actinopterygii Species 0.000 description 6
- 206010053759 Growth retardation Diseases 0.000 description 4
- 231100000001 growth retardation Toxicity 0.000 description 4
- 241000264847 Siniperca chuatsi Species 0.000 description 3
- 230000003111 delayed effect Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000001788 irregular Effects 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000005452 bending Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000594011 Leuciscus leuciscus Species 0.000 description 1
- 241000233667 Saprolegnia Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000035613 defoliation Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000013933 post-embryonic development Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for improving mandarin fish fertilization hatchability and seed uniformity, belonging to the technical field of mandarin fish breeding and comprising the following steps: during mandarin fish egg hatching, when the film emergence reaches half, a method of stopping water for 20-30 minutes is adopted, the emergence rate can be rapidly increased, the aberration rate is greatly reduced, and the uniformity and the opening rate of the mandarin fish seedlings are improved.
Description
Technical Field
The invention relates to the technical field of mandarin fish breeding, in particular to a method for improving mandarin fish fertilization egg hatchability and fry uniformity, which can improve mandarin fish fertilization egg hatchability, reduce fry distortion rate and greatly improve mandarin fish larval uniformity and opening rate. The method is also suitable for solving the problems of high deformity rate, low hatching rate and the like caused by the delay of the demoulding of the viscous eggs when the fishes are hatched at low temperature.
Background
The mandarin fish fertilized egg hatching is at 18-22 ℃ and the mandarin fish is in second and third ripeness in the same year, irregular development, low hatching rate, high distortion rate and low shedding rate are common bottleneck problems in mandarin fish breeding, so that the emergence rate is reduced by 40-70%, and the bait fish consumption is increased by 50-100%. Delayed demoulding is the most prominent cause of this bottleneck problem.
The diameter of mature mandarin fish egg is about 2.0mm, water absorption is 3.6-4.0 mm during hatching fertilized eggs, and is far less than that of drifting eggs (such as large quadrate fish eggs, dace eggs and the like), so that the eggs are heavy, the egg membranes are thick, and the flow rate is also increased during hatching; the oxygen consumption rate and the induction points of the mandarin fish embryo development and the postembryonic development are much higher than those of common fishes, and the hatching density is only 1/(10-30) of that of drifting eggs during large-scale hatching; when the membrane is removed, the enrichment of the membrane-dissolving enzyme which can promote the dissolution of the egg membrane is lower than that of the drifting egg, and the membrane is slowly dissolved when the membrane is removed due to the thickness of the egg membrane, and the eggs delayed by membrane breaking are easy to be stained with impurities and infected with saprolegnia, so that the hardening of the egg membrane is caused, the membrane breaking is more difficult, and the membrane breaking period is further prolonged. The above problems easily result in: the method comprises the following steps of delaying demoulding of mandarin fish fries for 6-12 hours (about 10-20%), delaying opening for 6-10 hours, causing irregular specification of mandarin fish fries, and increasing difficulty in later-stage seedling cultivation; delaying the demoulding of mandarin fish fries for 12-24 h (about 10-20%), delaying the opening for 10-20 h, and if no separately matched palatable bait fish exists, the survival rate of the opening is lower except for irregular mandarin fish fries; the delayed demoulding of mandarin fish fry over 24 hr (10-20%) is curved in egg, and has partial fry, curved trunk or tail, abnormal development and abnormal shape, and poor survival ability.
Disclosure of Invention
In order to overcome the problems, the invention designs a method for improving mandarin fish fertilized egg hatchability and fingerling regularity, which can improve mandarin fish fertilized egg hatchability, reduce fingerling distortion rate and greatly improve mandarin fish larval regularity and opening rate.
The invention adopts the following technical scheme:
during mandarin fish egg hatching, when the membrane emergence reaches half, a method of stopping filling water in a hatching container for 20-30 minutes is adopted, so that mandarin fish eggs are deposited at the bottom, and the eggs are mutually extruded and fixed, so that the hatching rate can be remarkably improved to 99-100%, the growth retardation rate is reduced to 1-5%, and the distortion rate is reduced to 0.5-2%; the aperture opening ratio is improved to 95 to 99 percent.
The Siniperca chuatsi egg in the deciduate period can not be thinned and dissolved automatically like a drifting egg membrane, the tail of the Siniperca chuatsi in the egg is thin and weak, and the Siniperca chuatsi can rupture the membrane and swim out only by twisting the trunk and swinging the tail. When eggs are suspended in water, the twisting of the trunk and the swinging of the tail of the mandarin fish can cause the rolling of the eggs, and the membrane can be broken in a long time, 20-30% of the membrane can be broken in 2 hours, and 50-60% of the membrane can be broken in 4 hours; when the ovum is sinking in the bottom, because the hard support of bottom and frictional force effect, the rupture of membranes accelerates, rupture of membranes efficiency increases substantially: 50-60% of the film is broken in 2 hours, and 80-90% of the film is broken in 4 hours; after fixing the eggs, the mandarin fish can rupture membranes in a short time: and (5) breaking the membrane for 2 hours by 95-100%.
The recommended water shut-down times for different incubation conditions are given in table 1 below.
TABLE 1 recommended Water cut-off times under different incubation conditions
Has the advantages that: compared with the prior art, the method for improving mandarin fish fertilized egg hatchability and seed uniformity has the following beneficial effects:
1. compared with the traditional breeding method, the method provided by the invention can quickly improve the yield of the planted seedlings;
2. the deformity rate is greatly reduced, and the deformity caused by trunk bending and caudal peduncle bending caused by demoulding delay is basically eliminated;
3. improves the uniformity and the opening rate of the mandarin fish.
Detailed Description
The invention will now be further illustrated by reference to examples, and detailed embodiments and specific procedures are given:
using an effective capacity of 9m3After artificial insemination (the operation time is required to be controlled within two hours), the mandarin fish is put into a loop and mixed evenly, and after full water absorption is carried out for 4 hours, the amount of the water is 10 ten thousand grains/m3And (5) normally hatching in 4 hatching loops. Timing when the fish eggs are demoulded by 50 percent, stopping water for 0, 10, 20 and 30 minutes, and then opening the waterAnd (4) observing and counting results of the membrane removal rate of 4 groups of different temperatures and spawning batches at 40 minutes (see table 2). Results of the demoulding rate, the growth retardation rate, the aberration rate and the opening rate of the 4 groups were observed and counted after the opening of the circular channel for 24 hours (see table 3).
TABLE 2 stripping rates at different temperatures and different water cut-off times for spawning batches
TABLE 3 defoliation rates, growth retardation rates, distortion rates and opening rates at different water-cut times 24 hours after opening of loop decoilation
Hatching loop 10 ten thousand grains/m3Under the incubation condition, water is cut off for 20-30 minutes, the film yield of the experimental group reaches 99-100%, and is improved by 3-15% compared with that of the control group; the growth retardation of the mandarin fish is reduced from 14-20% of a control group to 1-5% of an experimental group; the aberration rate is reduced from 10-15% of a control group to 0.5-2% of an experimental group; the opening rate is improved from 80-90% of a control group to 95-99% of an experimental group. The actual seedling emergence amount of each experimental group is improved by 50-100% compared with that of a control group, and the uniformity experimental group of the mandarin fish seedlings is far higher than that of the control group.
Claims (3)
1. A method for improving mandarin fish fertilized egg hatchability and seed uniformity is characterized in that: during mandarin fish egg hatching, when the membrane is half removed, water filling is stopped in the hatching container to collect mandarin fish egg at the bottom and fix the mandarin fish eggs via mutual extrusion.
2. The method for improving mandarin fish fertilized egg hatchability and seed uniformity as claimed in claim 1, wherein: the hatching container is a hatching barrel or a hatching loop.
3. The method for improving mandarin fish fertilized egg hatchability and seed uniformity as claimed in claim 1, wherein: the water filling stopping time is 20-30 minutes after water supply is stopped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011150618.8A CN112167124A (en) | 2020-10-24 | 2020-10-24 | Method for improving hatching rate of mandarin fish fertilized eggs and uniformity of seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011150618.8A CN112167124A (en) | 2020-10-24 | 2020-10-24 | Method for improving hatching rate of mandarin fish fertilized eggs and uniformity of seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112167124A true CN112167124A (en) | 2021-01-05 |
Family
ID=73922642
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011150618.8A Pending CN112167124A (en) | 2020-10-24 | 2020-10-24 | Method for improving hatching rate of mandarin fish fertilized eggs and uniformity of seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112167124A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103712A (en) * | 2007-08-03 | 2008-01-16 | 天津农学院 | Ietalurus punetaus artificial hatching method |
| CN103076283A (en) * | 2013-01-17 | 2013-05-01 | 中国水产科学研究院黑龙江水产研究所 | Method for observing developmental process of salmonidae roes |
| CN109392783A (en) * | 2018-10-11 | 2019-03-01 | 安徽瀚沣渔业科技发展有限公司 | A kind of fishery cultivating ovum support |
| CN111134054A (en) * | 2020-02-18 | 2020-05-12 | 南宁学院 | Method for increasing survival rate of rainbow trout eyed eggs |
| CN111316936A (en) * | 2018-12-13 | 2020-06-23 | 射阳县周正海家庭农场 | Static water stripping artificial propagation method for large-scale barbel fish |
-
2020
- 2020-10-24 CN CN202011150618.8A patent/CN112167124A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103712A (en) * | 2007-08-03 | 2008-01-16 | 天津农学院 | Ietalurus punetaus artificial hatching method |
| CN103076283A (en) * | 2013-01-17 | 2013-05-01 | 中国水产科学研究院黑龙江水产研究所 | Method for observing developmental process of salmonidae roes |
| CN109392783A (en) * | 2018-10-11 | 2019-03-01 | 安徽瀚沣渔业科技发展有限公司 | A kind of fishery cultivating ovum support |
| CN111316936A (en) * | 2018-12-13 | 2020-06-23 | 射阳县周正海家庭农场 | Static water stripping artificial propagation method for large-scale barbel fish |
| CN111134054A (en) * | 2020-02-18 | 2020-05-12 | 南宁学院 | Method for increasing survival rate of rainbow trout eyed eggs |
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Application publication date: 20210105 |