CN112033743A - Parkinson's excrement and urine liquid extraction element - Google Patents

Parkinson's excrement and urine liquid extraction element Download PDF

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CN112033743A
CN112033743A CN202010686144.2A CN202010686144A CN112033743A CN 112033743 A CN112033743 A CN 112033743A CN 202010686144 A CN202010686144 A CN 202010686144A CN 112033743 A CN112033743 A CN 112033743A
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杨卫廷
黄东雅
蒋艳艳
王璐
胡晖
顾晓波
余飞
鲍艺文
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Abstract

本发明公开了一种帕金森粪便液提取装置,其技术方案要点是包括有装置外壳和搅拌装置和抽取装置以及承载盘,装置外壳内设置有存储腔,装置外壳上设置有用于封堵存储腔的密封盖,搅拌装置包括有位于存储腔内用的储物管、和可拆卸安装于储物管上并且用于装纳样品粪便的过滤件、和可拆卸安装于储物管上的搅拌件、以及设置于密封盖上用于驱动搅拌件搅拌过滤件内样品粪便的驱动电机,储物管上设置有供粪便液流出至存储腔内的泻液孔,装置外壳上设置有用于持续将稀释液输入至过滤件内的循环装置,循环装置和搅拌件工作过程中粪便液持续从过滤件中流向存储腔内并且与稀释液交融,该提取装置能够自动进行搅拌、洗涤、提取操作,方便清洗。

Figure 202010686144

The invention discloses a Parkinson's fecal liquid extraction device. The technical solution of the device includes a device shell, a stirring device, an extraction device and a carrying plate. The device shell is provided with a storage cavity, and the device shell is provided with a storage cavity for sealing The sealing cover, the stirring device includes a storage tube for use in the storage cavity, a filter member detachably installed on the storage tube and used to accommodate the sample stool, and a stirring member detachably installed on the storage tube , and a drive motor arranged on the sealing cover for driving the stirring element to stir the sample feces in the filter element, the storage tube is provided with a laxative hole for the fecal liquid to flow out into the storage cavity, and the device shell is provided with a continuous dilution The liquid is input to the circulation device in the filter element. During the working process of the circulation device and the stirring element, the fecal liquid continues to flow from the filter element to the storage cavity and blends with the diluent. The extraction device can automatically perform stirring, washing and extraction operations, which is convenient for cleaning. .

Figure 202010686144

Description

一种帕金森粪便液提取装置A Parkinson's fecal fluid extraction device

技术领域technical field

本发明涉及一种提取装置,更具体地说,它涉及一种帕金森粪便液提取装置。The present invention relates to an extraction device, more particularly, to a Parkinson's fecal fluid extraction device.

背景技术Background technique

PD(Parkinson disease,帕金森病)是一种常见于中老年患者的中枢神经系统退行性疾病,我国65岁以上人群发病率约为1.7%。该病起病缓慢,呈慢性进行性发展,除了典型的静止性震颤、肌强直、运动减少和姿势步态异常等运动症状外,还存在以便秘、嗅觉丧失为主的自主神经功能紊乱而影响患者日常生活。已有研究显示和健康对照组相比PD患者普雷沃氏菌科数量减少;与以震颤为主的PD患者相比,姿势步态障碍为主的PD患者肠杆菌科菌群的数量远远增多,其他菌种如疣微菌科、慢生根瘤菌科、梭菌属Ⅳ、瘤胃菌科等在PD患者粪便中也有一定程度变化,因此,帕金森病患者存在肠道菌群失调,不同症状可能具有不同特征的菌群失调现象。PD (Parkinson disease, Parkinson's disease) is a degenerative disease of the central nervous system common in middle-aged and elderly patients. The incidence rate of people over 65 years old in my country is about 1.7%. In addition to the typical motor symptoms such as resting tremor, muscle rigidity, decreased movement, and abnormal posture and gait, the disease has a slow onset and a chronic progressive development. daily life of the patient. Previous studies have shown that the number of Prevotella in PD patients is reduced compared with healthy controls; compared with PD patients with tremor predominant, PD patients with postural gait disorders have a far greater number of Enterobacteriaceae increased, and other bacterial species such as Verrucomicrobiaceae, Brachyrhizobacteriaceae, Clostridium IV, Ruminobacteriaceae, etc. also changed to a certain extent in the feces of PD patients. Therefore, patients with Parkinson's disease have intestinal flora imbalance. Symptoms may have different characteristics of dysbiosis.

焦磷酸测序是一种新型的酶联级测序技术,对已知的短序列的测序分析,该技术既可以满足对重要微生物的鉴定与分型,又可以对特定DNA片段的突变进行检测和克隆。故可以对粪便指定菌群的微生物进行DNA检测,判断该微生物的种类及含量,通过对粪便菌群的监测,判断PD患者的病情走向,从而合理的做出预防操作。然而现有的装置存在以下问题:1.从搅拌、过滤、提取、转送,流程复杂,不仅耗时耗力而且浪费不少的人力资源;2.清洁装置的时候由于粪便滤渣具有一定粘性,造成后期清理麻烦。Pyrosequencing is a new type of enzyme-linked sequencing technology. The sequencing analysis of known short sequences can not only meet the identification and typing of important microorganisms, but also detect and clone mutations in specific DNA fragments. . Therefore, it is possible to perform DNA detection on the microorganisms of the designated fecal flora to determine the type and content of the microorganisms. However, the existing devices have the following problems: 1. From stirring, filtration, extraction, and transfer, the process is complicated, which is not only time-consuming and labor-intensive, but also wastes a lot of human resources; 2. When cleaning the device, the fecal filter residue has a certain viscosity, causing Trouble with cleaning up later.

发明内容SUMMARY OF THE INVENTION

针对现有技术存在的不足,本发明的目的在于提供一种帕金森粪便液提取装置,该提取装置能够自动进行搅拌、洗涤、提取操作,方便清洗。In view of the deficiencies in the prior art, the purpose of the present invention is to provide a Parkinson's fecal liquid extraction device, which can automatically perform stirring, washing, and extraction operations, and is convenient for cleaning.

为实现上述目的,本发明提供了如下技术方案:一种帕金森粪便液提取装置,包括有装置外壳、和设置于装置外壳内用于生成粪便液并进行过滤操作的搅拌装置、和用于抽取粪便液的抽取装置、以及用于承载容纳粪便液并且能够直接转放于焦磷酸测序装置内的承载盘;In order to achieve the above purpose, the present invention provides the following technical solutions: a Parkinson's fecal liquid extraction device, comprising a device casing, a stirring device arranged in the device casing for generating fecal liquid and performing a filtering operation, and a stirring device for extracting the fecal liquid. An extraction device for fecal fluid, and a carrier plate for carrying fecal fluid and capable of being directly transferred into the pyrosequencing device;

所述装置外壳内设置有用于存放稀释液的存储腔,所述装置外壳上设置有用于封堵存储腔的密封盖,所述搅拌装置包括有位于存储腔内并悬放于稀释液上方的储物管、和可拆卸安装于储物管上并且用于装纳样品粪便的过滤件、和可拆卸安装于储物管上的搅拌件、以及设置于密封盖上用于驱动搅拌件搅拌过滤件内样品粪便的驱动电机,所述储物管上设置有供粪便液流出至存储腔内的泻液孔,所述装置外壳上设置有用于持续将稀释液输入至过滤件内的循环装置,循环装置和搅拌件工作过程中粪便液持续从过滤件中流向存储腔内并且与稀释液交融。The device shell is provided with a storage cavity for storing the diluent, the device shell is provided with a sealing cover for blocking the storage cavity, and the stirring device includes a storage cavity located in the storage cavity and suspended above the diluent. A material pipe, a filter element detachably mounted on the storage pipe and used to accommodate the sample stool, a stirring element detachably mounted on the storage pipe, and a sealing cover for driving the stirring element to stir the filter element The drive motor for the sample feces, the storage tube is provided with a laxative hole for the fecal liquid to flow out into the storage cavity, and the device shell is provided with a circulation device for continuously inputting the diluent into the filter element. During the operation of the device and the stirring element, the fecal fluid continues to flow from the filter element to the storage cavity and blends with the diluent.

本发明进一步设置为:所述装置外壳上设置有用于控制密封盖沿装置外壳高度方向移动的控制装置,所述搅拌件包括有安装于储物管开口位置处的固定片和安装于驱动电机转轴上的搅拌轴,所述固定片滑移连接于搅拌轴上并且所述固定片上设置有径长尺寸大于搅拌轴径长尺寸的搅拌通孔,所述固定片上还设置有供循环装置向过滤件内输液的输液孔。The present invention is further provided as follows: the device casing is provided with a control device for controlling the movement of the sealing cover along the height direction of the device casing, and the stirring element includes a fixed piece installed at the opening of the storage tube and a rotating shaft installed on the drive motor On the stirring shaft, the fixed piece is slidably connected to the stirring shaft, and the fixed piece is provided with a stirring through hole whose diameter and length are greater than the diameter and length of the stirring shaft, and the fixed piece is also provided with a circulating device to the filter element. Infusion hole for internal infusion.

本发明进一步设置为:所述储物管通过若干连接杆悬浮于存储腔内,并且所述连接杆滑移连接于装置外壳的内侧壁上,所述装置外壳内侧壁上设置有若干供连接杆沿装置外壳高度方向滑移的滑移槽,所述滑移槽内设置有用于限制连接杆位置的限位件,所述固定片朝向密封盖的一侧凸设有抵接块,所述抵接块沿密封盖周向延伸并且呈下上起伏波浪状结构设置,所述搅拌轴上设置有与抵接块抵接的推动块,搅拌轴转动过程中储物管上下往返运动。The present invention further provides that: the storage tube is suspended in the storage cavity through a plurality of connecting rods, and the connecting rods are slidably connected to the inner side wall of the device shell, and a plurality of connecting rods are provided on the inner side wall of the device shell. A sliding groove that slides along the height direction of the device casing, a limiter for limiting the position of the connecting rod is arranged in the sliding groove, and abutting block is protruded from the side of the fixing piece facing the sealing cover, and the abutting block is arranged in the sliding groove. The connecting block extends along the circumferential direction of the sealing cover and is arranged in a wave-like structure. The stirring shaft is provided with a pushing block abutting against the abutting block.

本发明进一步设置为:所述循环装置包块有具有定时功能的循环液压泵和用于将存储腔内的稀释液输送至过滤件内的输液管,所述固定片远离密封盖的一侧设置有供输液管插入的限位管,所述限位管呈圆台结构设置,并且限位管端面大的一端与输液孔重合连通设置,所述限位管端面小的一端其径长尺寸小于输液管的径长尺寸。The present invention is further provided that: the circulation device package has a circulating hydraulic pump with a timing function and an infusion pipe for delivering the diluent in the storage cavity to the filter element, and the fixing piece is arranged on the side away from the sealing cover There is a limit tube for the infusion tube to be inserted into, the limit tube is arranged in a circular truncated structure, and the end of the limit tube with the large end face is overlapped and connected to the infusion hole, and the end of the limit tube with a small end face is smaller in diameter and length than the infusion. The diameter and length of the tube.

本发明进一步设置为:所述过滤件包括有用于安装至储物管开口位置的第一过滤袋、和设置于第一固定袋内并且安装至储物管开口位置的过滤管、以及设置于过滤管内用于放置样品粪便的第二过滤袋,所述过滤管上设置有若干过滤细孔,所述输液孔的位置与第二过滤袋开口的位置对应设置,所述储物管开口位置处设置有向上凸起的扣块,所述过滤管和第一过滤袋以及固定片上均设置有与扣块扣接的扣孔。The present invention further provides that: the filter element includes a first filter bag for being installed at the opening position of the storage tube, a filter tube disposed in the first fixed bag and installed at the opening position of the storage tube, and a filter tube disposed at the opening position of the storage tube. A second filter bag for placing sample feces in the tube, the filter tube is provided with a number of filter pores, the position of the infusion hole is set corresponding to the position of the opening of the second filter bag, and the position of the opening of the storage tube is set There is an upwardly protruding buckle block, and the filter tube, the first filter bag and the fixing sheet are all provided with button holes for buckled connection with the buckle block.

本发明进一步设置为:所述抽取装置包括有用于抽取稀释后的粪便液的抽液管、和用于给抽液管提供抽液动力的抽液泵、以及设置在装置外壳上的出液部,所述出液部的下端均匀排设有若干出液孔,所述出液孔内均设置有出液管,当抽液泵运行过程中抽液管内的液体均匀流向若干出液管内。The present invention is further provided that: the extraction device includes a liquid suction pipe for extracting the diluted fecal liquid, a liquid suction pump for providing liquid suction power to the liquid suction pipe, and a liquid outlet part arranged on the device shell The lower end of the liquid outlet is evenly arranged with a number of liquid outlet holes, and the liquid outlet holes are all provided with a liquid outlet pipe. When the liquid suction pump is running, the liquid in the liquid suction pipe flows evenly into the several liquid outlet pipes.

本发明进一步设置为:所述承载盘上均匀排设有若干用于容纳稀释后的粪便液的容纳槽,所述装置外壳上设置有用于放置承载盘的承载平台,所述承载平台上设置有若干与容纳槽一一对应的固定槽,所述出液部沿装置外壳的高度方向滑移设置,出液部滑移到位时出液孔与容纳槽一一对应。The present invention is further provided as follows: a plurality of accommodating grooves for accommodating diluted fecal liquid are evenly arranged on the carrying plate, a carrying platform for placing the carrying plate is arranged on the outer casing of the device, and a carrying platform is arranged on the carrying platform There are several fixing grooves corresponding to the accommodating grooves one-to-one, the liquid outlet part is arranged to slide along the height direction of the device shell, and the liquid outlet holes correspond to the accommodating grooves one-to-one when the liquid outlet part slides in place.

本发明进一步设置为:所述承载平台上设置有用于控制驱动电机和循环装置以及抽液泵启动的第一重力感应开关、和用于控制驱动电机和循环装置以及抽液泵关闭的第二重力感应开关,当承载盘放置于承载平台上时第一重力感应开关被触发并且循环装置和驱动电机依次启动并在指定时间后抽液泵将稀释后的粪便液抽取至容纳槽内,当容纳槽内液体达到指定重量时第二重力感应开关被触发抽液泵关闭。The present invention is further provided as follows: the bearing platform is provided with a first gravity induction switch for controlling the driving motor, the circulation device and the start of the suction pump, and a second gravity switch for controlling the driving motor, the circulation device and the closing of the suction pump Induction switch, when the carrier plate is placed on the carrier platform, the first gravity induction switch is triggered and the circulation device and the drive motor are started in sequence, and after a specified time, the suction pump will extract the diluted fecal liquid into the holding tank. When the internal liquid reaches the specified weight, the second gravity sensing switch is triggered to close the pump.

综上所述,本发明具有以下有益效果:1、自动进行搅拌操作,而且将搅拌后的稀释粪便液直接注入到承载盘上,减少了手动注入到焦磷酸测序装置内的步骤,方便快捷,机械注入也减少了人为注入的失误率;2、过滤件和搅拌件该类直接触及粪便的,由于粪便具有一定粘性,在使用过后,搅拌件能够直接扔掉,过滤件也能够直接扔掉,或者作为其他实验物资保留。3、循环装置能够将稀释液从上至下灌注到过滤件内,充分与样品粪便接触,保证搅拌的均匀性,除此之外,将储物管设计在位于稀释液的上方位置处,能够使得从储物管内流出的液体和位于其下方的稀释液充分混合,从而在长时间循环过程中,形成一均匀的稀释粪便也。该发明,操作方便,最终液体生成均匀。而且整个装置内没有粪便黏贴物,仅对粪便液进行清洗操作,相对于现有技术的不足,该设备清洗方便,快捷。To sum up, the present invention has the following beneficial effects: 1. The stirring operation is automatically performed, and the diluted fecal liquid after stirring is directly injected into the carrier plate, which reduces the steps of manually injecting into the pyrosequencing device, which is convenient and quick, Mechanical injection also reduces the error rate of artificial injection; 2. Filters and stirring pieces that directly touch the feces, because the feces have a certain viscosity, after use, the stirring piece can be thrown away directly, and the filter piece can also be thrown away directly, Or keep it as other experimental materials. 3. The circulation device can pour the diluent into the filter element from top to bottom, and fully contact with the sample feces to ensure the uniformity of stirring. The liquid flowing out of the storage tube and the diluent located below it are fully mixed, so as to form a uniform diluted feces during the long-term circulation process. The invention is convenient to operate, and the final liquid is evenly generated. In addition, there is no fecal sticker in the whole device, and only the fecal liquid is cleaned. Compared with the shortcomings of the prior art, the device is convenient and fast to clean.

附图说明Description of drawings

图1为帕金森粪便液提取装置的剖视示意图;Fig. 1 is the sectional schematic diagram of Parkinson's fecal fluid extraction device;

图2为搅拌装置的剖视示意图;2 is a schematic cross-sectional view of a stirring device;

图3为过滤件的剖视示意图。FIG. 3 is a schematic cross-sectional view of the filter element.

附图标记:1、装置外壳;11、存储腔;12、密封盖;13、循环装置;131、循环液压泵;132、输液管;14、连接杆;15、滑移槽;151、限位件;16、承载平台;161、固定槽;2、搅拌装置;21、储物管;211、泻液孔;22、过滤件;221、第一过滤袋;222、过滤管;223、第二过滤袋;23、搅拌件;231、固定片;2311、搅拌通孔;2312、输液孔;2313、抵接块;2314、限位管;232、搅拌轴;2321、推动块;24、驱动电机;3、抽取装置;31、抽液管;32、抽液泵;33、出液部;331、出液孔;332、出液管;4、承载盘;41、容纳槽。Reference numerals: 1. Device housing; 11. Storage cavity; 12. Sealing cover; 13. Circulation device; 131. Circulating hydraulic pump; 132, Infusion tube; parts; 16, carrying platform; 161, fixing tank; 2, stirring device; 21, storage tube; 211, laxative hole; 22, filter part; 221, first filter bag; 222, filter tube; 223, second filter bag; 23, stirring piece; 231, fixing piece; 2311, stirring through hole; 2312, infusion hole; 2313, abutment block; 2314, limit tube; 232, stirring shaft; 2321, push block; 24, drive motor 3. Extraction device; 31. Liquid suction pipe; 32. Liquid suction pump; 33. Liquid outlet; 331, Liquid outlet hole; 332, Liquid outlet pipe;

具体实施方式Detailed ways

下面结合附图和实施例,对本发明进一步详细说明。其中相同的零部件用相同的附图标记表示。需要说明的是,下面描述中使用的词语“前”、“后”、“左”、“右”、“上”和“下”指的是附图中的方向,词语“底面”和“顶面”、“内”和“外”分别指的是朝向或远离特定部件几何中心的方向。The present invention will be described in further detail below with reference to the accompanying drawings and embodiments. Wherein the same parts are denoted by the same reference numerals. It should be noted that the words "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to directions in the drawings, and the words "bottom" and "top" "Face", "inner" and "outer" refer to directions toward or away from the geometric center of a particular part, respectively.

参照图1至图3所示,为实现上述目的,本发明提供了如下技术方案:一种帕金森粪便液提取装置,包括有装置外壳1、和设置于装置外壳1内用于生成粪便液并进行过滤操作的搅拌装置2、和用于抽取粪便液的抽取装置3、以及用于承载容纳粪便液并且能够直接转放于焦磷酸测序装置内的承载盘4;1 to 3, in order to achieve the above purpose, the present invention provides the following technical solutions: a Parkinson's fecal liquid extraction device, comprising a device housing 1 and a device housing 1 for generating fecal liquid and The stirring device 2 for filtering operation, the extraction device 3 for extracting fecal liquid, and the carrier plate 4 for carrying the fecal liquid and capable of being directly transferred into the pyrosequencing device;

装置外壳1内设置有用于存放稀释液的存储腔11,装置外壳1上设置有用于封堵存储腔11的密封盖12,搅拌装置2包括有位于存储腔11内并悬放于稀释液上方的储物管21、和可拆卸安装于储物管21上并且用于装纳样品粪便的过滤件22、和可拆卸安装于储物管21上的搅拌件23、以及设置于密封盖12上用于驱动搅拌件23搅拌过滤件22内样品粪便的驱动电机24,储物管21上设置有供粪便液流出至存储腔11内的泻液孔211,装置外壳1上设置有用于持续将稀释液输入至过滤件22内的循环装置13,循环装置13和搅拌件23工作过程中粪便液持续从过滤件22中流向存储腔11内并且与稀释液交融。The device housing 1 is provided with a storage cavity 11 for storing the diluent, the device housing 1 is provided with a sealing cover 12 for sealing the storage cavity 11, and the stirring device 2 includes a storage cavity 11 and suspended above the diluent. The storage tube 21, the filter member 22 detachably installed on the storage tube 21 and used to accommodate the sample stool, and the stirring member 23 detachably installed on the storage tube 21, and provided on the sealing cover 12 for The drive motor 24 for stirring the sample feces in the filter element 22 is driven by the stirring element 23. The storage tube 21 is provided with a laxative hole 211 for the fecal liquid to flow out into the storage cavity 11. It is input to the circulation device 13 in the filter element 22. During the operation of the circulation device 13 and the stirring element 23, the fecal liquid continues to flow from the filter element 22 to the storage chamber 11 and blends with the diluent.

本装置操作步骤:1、将适量样品粪便放入过滤件22内,该过滤件22为一次性产品,用完即丢,所以避免清洗粪便的操作;The operation steps of the device: 1. Put an appropriate amount of sample feces into the filter element 22. The filter element 22 is a one-time product and is thrown away after use, so the operation of cleaning the feces is avoided;

2、将过滤件22放入储物管21内,并且将搅拌件23的一部分安装于储物管21上另一部分安装在驱动电机24上,该搅拌件23也为一次性产品,用完即丢,所以避免清洗粪便的操作;2. Put the filter element 22 into the storage pipe 21, and install a part of the stirring element 23 on the storage pipe 21 and the other part on the drive motor 24. The stirring element 23 is also a one-time product. Disposal, so avoid the operation of cleaning feces;

3、向存储腔11注入适量的稀释液,闭合密封盖12,并且将承载盘4放置于正确位置上;3. Pour an appropriate amount of diluent into the storage cavity 11, close the sealing cover 12, and place the carrier tray 4 in the correct position;

4、启动循环装置13,使得稀释液不管输送至过滤件22内;4. Start the circulation device 13, so that the diluent is transported into the filter element 22 regardless of;

5、启动驱动电机24使得搅拌件23不断搅拌过滤件22内的样品粪便,此时部分菌群随着稀释液从过滤件22流到储物管21再通过泻液孔211流至存储腔11内,与稀释液交融;5. Start the drive motor 24 so that the stirring member 23 continuously stirs the sample feces in the filter member 22. At this time, part of the flora flows from the filter member 22 to the storage tube 21 with the diluent and then flows to the storage cavity 11 through the laxative hole 211. inside, blended with the diluent;

6、持续进行4和5步骤,使得过滤件22内的样品粪便被充分分解,菌群全部与稀释液交融,此时稀释液变成稀释后的粪便液;6. Continue to carry out steps 4 and 5, so that the sample feces in the filter element 22 are fully decomposed, and the flora is all blended with the diluent, and the diluent becomes the diluted fecal liquid at this time;

7、停止循环装置13和驱动电机24,启动抽取装置3,将稀释后的粪便液抽取至承载盘4上;7. Stop the circulation device 13 and the drive motor 24, start the extraction device 3, and extract the diluted fecal liquid onto the carrier plate 4;

8、直接将容纳有稀释后的粪便液的承载盘4放置于焦磷酸测序装置内进行焦磷酸测序检测,检测出普雷沃氏菌科、肠杆菌科菌群、疣微菌科、慢生根瘤菌科、梭菌属Ⅳ、瘤胃菌科等含量。并且根据得出的菌群数值判断患者病情走向,从而做出有效医疗操作。8. Directly place the carrier plate 4 containing the diluted fecal fluid in the pyrosequencing device to perform pyrosequencing detection, and detect Prevotella, Enterobacteriaceae, Verrucobacterium, and Slow Rhizobacteria, Clostridium IV, Ruminaceae and other contents. And according to the obtained bacterial group value, the patient's condition is judged, so as to make effective medical operations.

本装置的清洗步骤:Cleaning steps for this device:

1、将过滤件22连带搅拌件23一并取下,丢弃;1. Remove the filter element 22 together with the stirring element 23 and discard it;

2、往存储腔11注水,将循环装置13开到最大,向储物管21内输水,由于最大功率的循环装置13的注水量大于储物管21的泄水量,能够逐渐淹没储物管21,进行循环洗涤;2. Fill the storage cavity 11 with water, turn on the circulation device 13 to the maximum, and deliver water into the storage pipe 21. Since the water injection volume of the circulation device 13 with the maximum power is greater than the discharge volume of the storage pipe 21, the storage pipe can be gradually submerged. 21. Perform circular washing;

3、启动抽取装置3,通过自主抽水,达到洗涤抽取装置3内部的效果。3. Start the extraction device 3, and achieve the effect of washing the interior of the extraction device 3 by pumping water autonomously.

本发明设计的好处:1、自动进行搅拌操作,而且将搅拌后的稀释粪便液直接注入到承载盘4上,减少了手动注入到焦磷酸测序装置内的步骤,方便快捷,机械注入也减少了人为注入的失误率;2、过滤件22和搅拌件23该类直接触及粪便的,由于粪便具有一定粘性,在使用过后,搅拌件23能够直接扔掉,过滤件22也能够直接扔掉,或者作为其他实验物资保留。3、循环装置13能够将稀释液从上至下灌注到过滤件22内,充分与样品粪便接触,保证搅拌的均匀性,如果直接在稀释液中采用搅拌件23搅拌过滤件22内的样品粪便,会使得内外液压差不便,由于在过滤件22的作用下,使得菌群集中位于过滤件22内的粪便液中,在后续抽取装置3工作过程中导致抽取液中菌群不够充分,造成实验误差。除此之外,将储物管21设计在位于稀释液的上方位置处,能够使得从储物管21内流出的液体和位于其下方的稀释液充分混合,从而在长时间循环过程中,形成一均匀的稀释粪便也。该发明,操作方便,最终液体生成均匀。而且整个装置内没有粪便黏贴物,仅对粪便液进行清洗操作,相对于现有技术的不足,该设备清洗方便,快捷。The advantages of the design of the present invention: 1. The stirring operation is performed automatically, and the diluted fecal liquid after stirring is directly injected into the carrier plate 4, which reduces the steps of manual injection into the pyrosequencing device, which is convenient and quick, and reduces the mechanical injection. The error rate of artificial injection; 2. The filter element 22 and the stirring element 23 directly touch the feces. Because the feces have a certain viscosity, after use, the stirring element 23 can be thrown away directly, and the filter element 22 can also be directly thrown away, or Reserved as other experimental materials. 3. The circulation device 13 can pour the diluent into the filter element 22 from top to bottom, and fully contact the sample feces to ensure the uniformity of stirring. , it will make the difference in internal and external hydraulic pressure inconvenient, because under the action of the filter element 22, the bacteria cluster is located in the fecal liquid in the filter element 22, and the bacteria group in the extraction liquid is not sufficient during the subsequent operation of the extraction device 3, resulting in the experiment error. In addition, the storage tube 21 is designed to be located above the diluent, so that the liquid flowing out of the storage tube 21 and the diluent located below it can be fully mixed, so that during the long-term circulation process, the formation of An evenly diluted stool also. The invention is convenient to operate, and the final liquid is evenly generated. In addition, there is no fecal sticker in the whole device, and only the fecal liquid is cleaned. Compared with the shortcomings of the prior art, the device is convenient and fast to clean.

装置外壳1上设置有用于控制密封盖12沿装置外壳1高度方向移动的控制装置,搅拌件23包括有安装于储物管21开口位置处的固定片231和安装于驱动电机24转轴上的搅拌轴232,固定片231滑移连接于搅拌轴232上并且固定片231上设置有径长尺寸大于搅拌轴232径长尺寸的搅拌通孔2311,固定片231上还设置有供循环装置13向过滤件22内输液的输液孔2312。固定片231用于封堵储物管21的开口,使得搅拌轴232在搅拌过程中粪便不会从过滤件22中飞溅出来,首先将固定片231安装于储物管21开口位置处,再将搅拌轴232安装至驱动电机24的转轴上,通过控制装置将密封盖12盖合在装置外壳1上时,搅拌轴232插入搅拌通孔2311内,当驱动电机24启动时,搅拌轴232搅拌过滤件22内的样品粪便,循环装置13通过输液孔2312向过滤件22内输入稀释液。The device housing 1 is provided with a control device for controlling the movement of the sealing cover 12 along the height direction of the device housing 1, and the stirring member 23 includes a fixed piece 231 installed at the opening position of the storage tube 21 and a stirring member installed on the rotating shaft of the drive motor 24. The shaft 232, the fixed piece 231 is slidably connected to the stirring shaft 232, and the fixed piece 231 is provided with a stirring through hole 2311 whose diameter and length are larger than the diameter and length of the stirring shaft 232, and the fixed piece 231 is also provided with a filter for the circulation device 13 to filter. The infusion hole 2312 in the component 22 for infusion. The fixing piece 231 is used to block the opening of the storage tube 21, so that the stirring shaft 232 will not splash out the feces from the filter element 22 during the stirring process. The stirring shaft 232 is installed on the rotating shaft of the driving motor 24. When the sealing cover 12 is closed on the device casing 1 by the control device, the stirring shaft 232 is inserted into the stirring through hole 2311. When the driving motor 24 is started, the stirring shaft 232 stirs and filters The sample feces in the filter element 22, the circulation device 13 inputs the diluent into the filter element 22 through the infusion hole 2312.

储物管21通过若干连接杆14悬浮于存储腔11内,并且连接杆14滑移连接于装置外壳1的内侧壁上,装置外壳1内侧壁上设置有若干供连接杆14沿装置外壳1高度方向滑移的滑移槽15,滑移槽15内设置有用于限制连接杆14位置的限位件151,固定片231朝向密封盖12的一侧凸设有抵接块2313,抵接块2313沿密封盖12周向延伸并且呈下上起伏波浪状结构设置,搅拌轴232上设置有与抵接块2313抵接的推动块2321,搅拌轴232转动过程中储物管21上下往返运动。该限位件151可以以限位弹簧的形式,通过连接杆14将储物管21悬浮于稀释液的上方,并且在搅拌轴232转动过程中通过推动块2321推动抵接块2313使得储物管21上下移动,从而达到更加全面的搅拌效果,使得内部搅拌更加充足。The storage tube 21 is suspended in the storage cavity 11 through a plurality of connecting rods 14 , and the connecting rods 14 are slidably connected to the inner side wall of the device housing 1 . The sliding groove 15 slides in the direction. A limiting member 151 for limiting the position of the connecting rod 14 is arranged in the sliding groove 15. The side of the fixing piece 231 facing the sealing cover 12 is protruded with an abutting block 2313. The abutting block 2313 It extends along the circumferential direction of the sealing cover 12 and is arranged in a wave-like structure. The limiting member 151 can be in the form of a limiting spring to suspend the storage tube 21 above the diluent through the connecting rod 14, and during the rotation of the stirring shaft 232, the abutment block 2313 is pushed by the pushing block 2321 to make the storage tube 21 21 moves up and down, so as to achieve a more comprehensive stirring effect and make the internal stirring more sufficient.

循环装置13包块有具有定时功能的循环液压泵131和用于将存储腔11内的稀释液输送至过滤件22内的输液管132,固定片231远离密封盖12的一侧设置有供输液管132插入的限位管2314,限位管2314呈圆台结构设置,并且限位管2314端面大的一端与输液孔2312重合连通设置,限位管2314端面小的一端其径长尺寸小于输液管132的径长尺寸。循环液压泵131与驱动电机24能够依次启动,定时功能的循环液压泵131能够针对不同状态的样品粪便进行定时操作,对于稍软一点的粪便进行较短时间的搅拌、循环操作,对于较硬一点的粪便进行较长时间的搅拌、循环操作。The circulation device 13 block is provided with a circulating hydraulic pump 131 with a timing function and an infusion tube 132 for conveying the diluent in the storage chamber 11 to the filter element 22. The side of the fixing sheet 231 away from the sealing cover 12 is provided with an infusion solution for infusion. The limit tube 2314 into which the tube 132 is inserted, the limit tube 2314 is arranged in a circular truncated structure, and the large end face of the limit tube 2314 is overlapped and communicated with the infusion hole 2312, and the end face of the limit tube 2314 is smaller in diameter and length than the infusion tube. 132 diameter and length. The circulating hydraulic pump 131 and the driving motor 24 can be started in sequence. The circulating hydraulic pump 131 with the timing function can perform timing operations for sample feces in different states. For slightly softer feces, a short period of stirring and circulation operations can be performed. The feces are stirred and circulated for a long time.

除此之外该限位管2314的作用:1、对输液管132起到限位作用,不会过分插入导致输液管132与样品粪便抵接;2、样品粪便在被搅拌轴232搅拌过程中不会飞溅到输液管132上。保证输液管132不会被污染。In addition, the function of the limiting tube 2314: 1. It has a limiting effect on the infusion tube 132, and will not cause the infusion tube 132 to abut the sample feces due to excessive insertion; 2. The sample feces are stirred by the stirring shaft 232 during the process Does not splash on the infusion tube 132. Ensure that the infusion tube 132 is not contaminated.

过滤件22包括有用于安装至储物管21开口位置的第一过滤袋221、和设置于第一固定袋内并且安装至储物管21开口位置的过滤管222、以及设置于过滤管222内用于放置样品粪便的第二过滤袋223,过滤管222上设置有若干过滤细孔,输液孔2312的位置与第二过滤袋223开口的位置对应设置,储物管21开口位置处设置有向上凸起的扣块,过滤管222和第一过滤袋221以及固定片231上均设置有与扣块扣接的扣孔。三层的过滤结构设计:1、第二过滤袋223过滤掉大颗粒粪球;2、过滤管222防止第二过滤袋223破损,并且过滤掉小颗粒粪球;3、用于过滤掉细小粪球;而且通过第一过滤袋221、过滤管222、第二过滤袋223能够生成相对应的粪球,从而应用到其他实验中。并且通过扣块和扣孔将第一过滤袋221、过滤管222、固定片231安装在储物管21上,在一切操作完毕之后,如果需要打包带离粪球,只需接触搅拌轴232的固定,再拉扯第一过滤袋221连带搅拌件23一并打包带走。The filter element 22 includes a first filter bag 221 installed at the opening position of the storage tube 21 , a filter tube 222 arranged in the first fixed bag and installed at the opening position of the storage tube 21 , and a filter tube 222 arranged in the filter tube 222 The second filter bag 223 for placing the sample feces, the filter tube 222 is provided with a number of filter pores, the position of the infusion hole 2312 is set corresponding to the position of the opening of the second filter bag 223, and the opening position of the storage tube 21 is provided with upwards. The raised buckle block, the filter tube 222 , the first filter bag 221 and the fixing plate 231 are all provided with button holes that are buckled with the buckle block. Three-layer filtration structure design: 1. The second filter bag 223 filters out large particles of fecal balls; 2. The filter tube 222 prevents the second filter bag 223 from being damaged, and filters out small particles of fecal balls; 3. Used to filter out small fecal balls And through the first filter bag 221, the filter tube 222, and the second filter bag 223, the corresponding fecal balls can be generated, which can be applied to other experiments. And the first filter bag 221, the filter tube 222, and the fixing piece 231 are installed on the storage tube 21 through the buckle block and the button hole. After all operations are completed, if you need to pack and remove the fecal balls, you only need to contact the stirring shaft 232. Fix it, and then pull the first filter bag 221 together with the stirring member 23 and pack it away.

抽取装置3包括有用于抽取稀释后的粪便液的抽液管31、和用于给抽液管31提供抽液动力的抽液泵32、以及设置在装置外壳1上的出液部33,出液部33的下端均匀排设有若干出液孔331,出液孔331内均设置有出液管332,当抽液泵32运行过程中抽液管31内的液体均匀流向若干出液管332内。均匀排设的出液孔331能够与承载盘4的容纳位置一一对应,并且通过抽液泵32将稀释后的粪便液均匀的注入到承载盘4上。避免了人为注入的不准确性和耗时大的缺点。The extraction device 3 includes a liquid suction pipe 31 for extracting the diluted fecal liquid, a liquid suction pump 32 for providing liquid suction power to the liquid suction pipe 31, and a liquid outlet 33 provided on the device casing 1, and the outlet is discharged. The lower end of the liquid part 33 is evenly arranged with a plurality of liquid outlet holes 331, and the liquid outlet holes 331 are all provided with a liquid outlet pipe 332. When the liquid suction pump 32 is running, the liquid in the liquid suction pipe 31 flows evenly to the plurality of liquid outlet pipes 332. Inside. The evenly arranged liquid outlet holes 331 can correspond one-to-one with the accommodating positions of the carrying tray 4 , and the diluted fecal liquid is evenly injected onto the carrying tray 4 through the liquid suction pump 32 . The inaccuracy and time-consuming disadvantages of artificial injection are avoided.

承载盘4上均匀排设有若干用于容纳稀释后的粪便液的容纳槽41,装置外壳1上设置有用于放置承载盘4的承载平台16,承载平台16上设置有若干与容纳槽41一一对应的固定槽161,出液部33沿装置外壳1的高度方向滑移设置,出液部33滑移到位时出液孔331与容纳槽41一一对应。该出液部33可以设计成功手动滑移也能够自动滑移,当出液部33远离承载平台16时,承载平台16上方空间充足,使用者方便将承载盘4放置于承载平台16上,当出液部33靠近承载平台16时,出液管332贴近容纳槽41,输液稳定,不易出现液体激荡的情况发生。A plurality of accommodating grooves 41 for accommodating the diluted fecal liquid are evenly arranged on the carrying plate 4, and a carrying platform 16 for placing the carrying plate 4 is arranged on the housing 1 of the device. With a corresponding fixing groove 161, the liquid outlet 33 is slidably disposed along the height direction of the device casing 1. When the liquid outlet 33 is slid in place, the liquid outlet holes 331 are in one-to-one correspondence with the accommodating grooves 41. The liquid outlet 33 can be designed to slide manually or automatically. When the liquid outlet 33 is far away from the carrying platform 16, the space above the carrying platform 16 is sufficient, and the user can conveniently place the carrying tray 4 on the carrying platform 16. When the liquid outlet 33 is close to the bearing platform 16, the liquid outlet pipe 332 is close to the accommodating tank 41, the infusion is stable, and the situation of liquid turbulence is less likely to occur.

承载平台16上设置有用于控制驱动电机24和循环装置13以及抽液泵32启动的第一重力感应开关、和用于控制驱动电机24和循环装置13以及抽液泵32关闭的第二重力感应开关,当承载盘4放置于承载平台16上时第一重力感应开关被触发并且循环装置13和驱动电机24依次启动并在指定时间后抽液泵32将稀释后的粪便液抽取至容纳槽41内,当容纳槽41内液体达到指定重量时第二重力感应开关被触发抽液泵32关闭。该第一重力感应开关和第二重力感应开关的设计,实现的自动一体化操作,大大方便了使用者的实用性。The carrying platform 16 is provided with a first gravity induction switch for controlling the drive motor 24, the circulation device 13 and the suction pump 32 to start, and a second gravity induction switch for controlling the drive motor 24, the circulation device 13 and the suction pump 32 to close. switch, when the carrier plate 4 is placed on the carrier platform 16, the first gravity-sensing switch is triggered and the circulation device 13 and the drive motor 24 are activated in sequence, and after a specified time, the liquid pump 32 will extract the diluted fecal liquid to the holding tank 41 Inside, when the liquid in the accommodating tank 41 reaches a specified weight, the second gravity sensing switch is triggered to turn off the pump 32 . The design of the first gravity sensing switch and the second gravity sensing switch realizes the automatic integrated operation, which greatly facilitates the practicability of the user.

除此之外为了排除患者由于食用了酸性食物导致最终检测结果出现偏差,在循环装置13内设置有检测稀释后的粪便液的检测试纸,再配合上能够直观显示检测试纸的检测结构的光谱仪,在抽取装置抽取时能够顺带检测粪便液的PH值,发现PH值异常时可以中断焦磷酸测序,避免造成更多的资源浪费。In addition, in order to eliminate the deviation of the final test result caused by the patient's consumption of acidic food, a test strip for detecting the diluted fecal liquid is provided in the circulation device 13, and a spectrometer that can visually display the detection structure of the test strip is installed. When the extraction device extracts, the pH value of the fecal fluid can be detected incidentally, and when the pH value is found to be abnormal, the pyrosequencing can be interrupted to avoid more waste of resources.

为了保证粪便始终在合适的环境内进行搅拌操作,在装置外壳1的存储腔11内设置控温装置,使得搅拌的各个阶段处于想要的温度环境中。In order to ensure that the feces are always stirred in a suitable environment, a temperature control device is provided in the storage cavity 11 of the device shell 1, so that each stage of stirring is in a desired temperature environment.

以上仅是本发明的优选实施方式,本发明的保护范围并不仅局限于上述实施例,凡属于本发明思路下的技术方案均属于本发明的保护范围。应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明原理前提下的若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。The above are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions that belong to the idea of the present invention belong to the protection scope of the present invention. It should be pointed out that for those skilled in the art, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims (8)

1. A Parkinson excrement liquid extraction device is characterized by comprising a device shell (1), a stirring device (2) which is arranged in the device shell (1) and used for generating excrement liquid and performing filtering operation, an extraction device (3) used for extracting the excrement liquid, and a bearing disc (4) used for bearing and containing the excrement liquid and capable of being directly placed in a pyrosequencing device in a rotating mode;
the device is characterized in that a storage cavity (11) for storing diluent is arranged in the device shell (1), a sealing cover (12) for sealing the storage cavity (11) is arranged on the device shell (1), the stirring device (2) comprises a storage pipe (21) which is positioned in the storage cavity (11) and is suspended above the diluent, a filtering piece (22) which is detachably installed on the storage pipe (21) and is used for containing sample excrement, a stirring piece (23) which is detachably installed on the storage pipe (21), and a driving motor (24) which is arranged on the sealing cover (12) and is used for driving the stirring piece (23) to stir the sample excrement in the filtering piece (22), a liquid discharging hole (211) for the excrement liquid to flow out into the storage cavity (11) is formed in the storage pipe (21), and a circulating device (13) which is used for continuously inputting the diluent into the filtering piece (22) is arranged on the device shell (1), during operation of the circulation device (13) and the stirring element (23), the fecal matter continues to flow from the filter element (22) into the storage chamber (11) and intermingle with the diluent.
2. The parkinson's disease liquid extraction device of claim 1, wherein: be provided with on device shell (1) and be used for controlling sealed lid (12) along the controlling means that device shell (1) direction of height removed, stirring piece (23) including install stationary blade (231) of storage pipe (21) opening position department and install in driving motor (24) epaxial (mixing) shaft (232) of rotating, stationary blade (231) slide connect on (mixing) shaft (232) and be provided with footpath length size on stationary blade (231) and be greater than (mixing) shaft (232) footpath length size's stirring through-hole (2311), still be provided with on stationary blade (231) and supply circulating device (13) to filter infusion hole (2312) of piece (22) internal infusion.
3. The parkinson's disease liquid extraction device of claim 2, wherein: the storage pipe (21) suspends in the storage cavity (11) through a plurality of connecting rods (14), and the connecting rods (14) are connected on the inside wall of the device shell (1) in a sliding manner, a plurality of sliding grooves (15) for the connecting rods (14) to slide along the height direction of the device shell (1) are formed in the inside wall of the device shell (1), limiting parts (151) used for limiting the positions of the connecting rods (14) are arranged in the sliding grooves (15), abutting blocks (2313) are convexly arranged on one sides of the fixing pieces (231) facing the sealing cover (12), the abutting blocks (2313) extend in the circumferential direction of the sealing cover (12) and are arranged in a wavy structure with upward and downward fluctuation, pushing blocks (2321) abutted to the abutting blocks (2313) are arranged on the stirring shaft (232), and the storage pipe (21) moves up and down in a reciprocating manner in the rotation process of the stirring shaft (232.
4. The parkinson's disease liquid extraction device of claim 2, wherein: the circulating device (13) package piece has circulating hydraulic pump (131) that has timing function and is used for carrying the weak solution in the storage chamber (11) to transfer line (132) in filtering piece (22), the stationary blade (231) keep away from sealed one side of lid (12) and is provided with spacing pipe (2314) that supply transfer line (132) to insert, spacing pipe (2314) are the setting of round platform structure to the one end that spacing pipe (2314) terminal surface is big and infusion hole (2312) coincidence intercommunication setting, its footpath length size of the little one end of spacing pipe (2314) terminal surface is less than the footpath length size of transfer line (132).
5. The parkinson's disease liquid extraction device of claim 4, wherein: filter piece (22) including be used for installing to storage pipe (21) open position first filter bag (221) and set up in first fixed bag and install to storage pipe (21) open position filter pipe (222) and set up in filter pipe (222) and be used for placing sample faecal second filter bag (223), be provided with a plurality of filtration pores on filter pipe (222), the position of infusion hole (2312) corresponds the setting with second filter bag (223) open position, storage pipe (21) open position department is provided with the bellied knot piece that makes progress, all be provided with the knot hole with knot piece lock joint on filter pipe (222) and first filter bag (221) and stationary blade (231).
6. The parkinson's disease liquid extraction device of claim 4, wherein: draw-out device (3) including be used for extracting liquid suction pipe (31) of the excrement and urine liquid after diluting and be used for providing drawing liquid power for liquid suction pipe (31) drawing liquid pump (32) and set up play liquid portion (33) on device shell (1), the lower extreme of play liquid portion (33) is evenly arranged and is equipped with a plurality of liquid holes (331), all be provided with drain pipe (332) in liquid hole (331), in liquid uniform flow direction a plurality of drain pipes (332) in liquid suction pipe (31) when drawing liquid pump (32) operation in-process.
7. The parkinson's disease liquid extraction device of claim 6, wherein: bear and evenly arrange holding tank (41) that are used for holding excrement and urine liquid after diluting on dish (4), be provided with on device shell (1) and be used for placing bearing platform (16) that bear dish (4), be provided with fixed slot (161) of a plurality of and holding tank (41) one-to-one on bearing platform (16), go out liquid portion (33) and slide the setting along the direction of height of device shell (1), go out liquid hole (331) and holding tank (41) one-to-one when liquid portion (33) slide and target in place.
8. The parkinson's disease liquid extraction device of claim 7, wherein: the carrying platform (16) is provided with a first gravity sensing switch for controlling the driving motor (24), the circulating device (13) and the liquid pump (32) to be started and a second gravity sensing switch for controlling the driving motor (24), the circulating device (13) and the liquid pump (32) to be closed, when the carrying plate (4) is placed on the carrying platform (16), the first gravity sensing switch is triggered, the circulating device (13) and the driving motor (24) are sequentially started, the liquid pump (32) pumps diluted excrement liquid into the accommodating groove (41) after a specified time, and when the liquid in the accommodating groove (41) reaches a specified weight, the second gravity sensing switch is triggered to close the liquid pump (32).
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