CN111961574A

CN111961574A - Cell separation device for biological field

Info

Publication number: CN111961574A
Application number: CN202010822024.0A
Authority: CN
Inventors: 吴永淇
Original assignee: Fuzhou Pengbo Biotechnology Co ltd
Current assignee: Fuzhou Pengbo Biotechnology Co ltd
Priority date: 2020-08-15
Filing date: 2020-08-15
Publication date: 2020-11-20

Abstract

The invention relates to the technical field of biology, and discloses a cell separation device used in the field of biology, which comprises a shell, wherein the outer side of the shell is fixedly connected with a storage frame; the automatic test-tube rack that vibrates, make the activity of cell obtain guaranteeing when promoting the cell separation, compare the method of stirring formula separation, effectively reduce the cracked probability of cell, make cell separation operation go on smoothly, according to actual need, automatic pause vibration operation, avoid frequent switchgear, increase equipment's burden, reduce equipment's life, and trigger the operation of elevating, increase the linkage nature between the structure, higher degree of automation has, make test-tube rack and put thing frame part break away from, the test tube exposes in use, can directly observe the condition in the test tube under the condition that need not to take, the trouble of artifical manual test tube observation of taking has been removed from, also can avoid causing the pollution because of the bacterium of hand to the separation sample, the effect of messenger's cell separation is guaranteed.

Description

Cell separation device for biological field

Technical Field

The invention relates to the technical field of biology, in particular to a cell separation device used in the field of biology.

Background

During the research of biological cells, whether the cell behavior is studied or the cell-related by-products are cultivated to meet the quantity and quality required by the research. Cell culture usually requires cell separation, stirring is a common separation method, but during operation, the cells may be broken due to excessive centrifugal force, and the cells are inactivated, so that the quality of cell separation is greatly reduced. During separation, cells need to be frequently observed, the progress of cell separation is known, and the equipment needs to be turned on and off for multiple times, so that the operation load of the equipment is increased, the equipment is burnt out, and the service life of the equipment is shortened. Moreover, the test tube is taken manually, so that the observation is troublesome, the risk of sample pollution is increased, and the cell separation effect is influenced.

Aiming at the defects of the prior art, the invention provides a cell separation device used in the biological field, which has the advantages of vibration separation, automatic lifting and convenient operation, solves the problems of poor separation effect, inconvenient operation and easy damage of equipment, automatically vibrates a test tube rack, promotes the cell separation and ensures the activity of the cells simultaneously, compared with a stirring type separation method, effectively reduces the probability of cell rupture, ensures the cell separation operation to be carried out smoothly, can automatically suspend the vibration operation according to the actual requirement, avoids frequent switching of the equipment, increases the load of the equipment, reduces the service life of the equipment, triggers the lifting operation to separate the test tube rack from a storage frame part, can directly observe the conditions in the test tube without taking the test tube when the test tube is exposed and used, avoids the trouble of manually taking the test tube for observation, the pollution of bacteria on hands to the separated sample can be avoided, and the cell separation effect is ensured.

Disclosure of Invention

Technical scheme (I)

In order to realize the purposes of vibration separation, automatic lifting and convenient operation, the invention provides the following technical scheme: a cell separation device for biological field includes shell, puts thing frame, test-tube rack, ejector pin, inversion V type pole, gear, first commentaries on classics board, carousel, inversion Z type pole, bar copper, magnetic pole group, ratchet, second commentaries on classics board, circle board

Wherein:

the position and connection relationship among the structures are as follows:

a cell separation device used in the biological field comprises a shell, wherein an arc-shaped through groove is formed in the surface of the shell, a storage frame is fixedly connected to the outer side of the shell, a through hole is formed in the bottom of the storage frame, a moving frame is movably connected to the inner portion of the storage frame, a test tube rack is movably connected to the inner portion of the moving frame, clamping plates are fixedly connected to the two sides of the test tube rack, three placing openings with the same size are formed in the test tube rack, an ejector rod is movably connected to the bottom of the test tube rack and is in an inverted L shape, a top plate is fixedly connected to the top of a vertical rod of the test tube rack, a T-shaped rod is movably connected to the outer side of a cross rod of the test tube rack, a through hole is formed in the cross rod of the T-shaped rod, an inverted V-shaped rod is fixedly connected to the bottom of the, the outside fixedly connected with sawtooth of down tube, the size looks adaptation of its size and gear, the outside swing joint of the V type pole of inversion has the gear, the outside swing joint of gear has the spring, the top swing joint of gear has first commentaries on classics board, the outside swing joint of first commentaries on classics board has first stay cord, one side swing joint that first commentaries on classics board was kept away from to first stay cord has the spring, first stay cord swing joint is in the outside of gear, the inside swing joint of shell has the carousel, the tooth is dialled to the outside fixedly connected with of carousel, the outside fixedly connected with lug and the push rod of carousel.

An inverted Z-shaped rod is movably connected below the rotary disc, an insulating rod is fixedly connected to the outer side of the inverted Z-shaped rod, a copper rod is fixedly connected to the bottom of the insulating rod, a copper rod is movably connected below the inverted Z-shaped rod, a magnetic pole group is fixedly connected to the outer side of the copper rod and consists of an N pole block and an S pole block, a fixed frame is fixedly connected to the outer side of the magnetic pole group, a clamping plate is fixedly connected to the top of the fixed frame, a spring is movably connected to the outer side of the fixed frame, a ratchet wheel is movably connected below the copper rod, a second rotary plate is movably connected to the outer side of the ratchet wheel, a spring is movably connected to the outer side of the second rotary plate, one side of the spring, which is far away from the second rotary plate, is movably connected with a rotary disc, one side of the rotary disc, which is far away from the second rotary plate, is movably connected with a ring plate, the outside fixedly connected with three dogs that the size is the same of circle board, the outside swing joint of circle board has the second stay cord, and one side that the circle board was kept away from to the second stay cord with put the removal frame swing joint in the thing frame.

(II) advantageous effects

Compared with the prior art, the invention provides a cell separation device used in the biological field, which has the following beneficial effects:

1. this a cell separation device for biological field through the combined action of test-tube rack, ratchet, second commentaries on classics board and circle board, vibrates the test-tube rack automatically, makes the activity of cell obtain guaranteeing when promoting cell separation, compares the method of stirring formula separation, effectively reduces the probability that the cell broke, makes cell separation operation go on smoothly.

2. This a cell separation device for biological field through the combined action of carousel, inversion Z type pole, bar copper, magnetic pole group, ratchet, second commentaries on classics board and circle board, according to actual need, automatic suspension vibration operation avoids frequent switchgear, increases the burden of equipment, reduces the life of equipment to trigger and rise the position operation, increase the linkage nature between the structure, have higher degree of automation.

3. This a cell separator for biological field through put thing frame, test-tube rack, ejector pin, inversion V type pole, gear and the first combined action that changes the board, makes the test-tube rack break away from with putting thing frame portion, and the test tube exposes in use, can the condition in the direct observation test tube under the condition that need not to take, has removed the trouble that the artifical manual test tube of taking observed from, also can avoid causing the pollution because of the bacterium of hand to the separation sample, makes the effect of cell separation obtain guaranteeing.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a cross-sectional view of FIG. 1 in accordance with the present invention;

FIG. 3 is a schematic view of the connection relationship between the carrier rod and the first rotating plate;

FIG. 4 is a schematic view of a structural ring plate of the present invention.

In the figure: 1. a housing; 2. a storage frame; 3. a test tube rack; 4. a top rod; 5. inverting the V-shaped rod; 6. a gear; 7. a first rotating plate; 8. a turntable; 9. inverting the Z-shaped rod; 10. a copper bar; 11. a magnetic pole group; 12. a ratchet wheel; 13. a second rotating plate; 14. and (7) a ring plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-4, a cell separation device for the biological field comprises a housing 1, an arc-shaped through groove is formed on the surface of the housing 1, the arc-shaped through groove is located on the outer side of a dial tooth of a rotary disc 8, a placement frame 2 is fixedly connected to the outer side of the housing 1, a through hole is formed in the bottom of the placement frame 2, a movable frame is movably connected to the inside of the placement frame 2, the movable frame is movably clamped in the placement frame 2, two sides of the movable frame are movably connected with two springs with the same specification, one side of each spring, which is far away from the movable frame, is movably connected with the inner wall of the housing 1, a test tube rack 3 is movably connected to the inside of the movable frame, a test tube rack 3 is movably connected to the inside of the placement frame 2, clamping plates are fixedly connected to two sides of the test tube rack 3, the movable clamping plates are movably clamped on, the size looks adaptation of its size and test tube, the bottom swing joint of test-tube rack 3 has ejector pin 4, ejector pin 4 presents and inverts the L form, the top fixedly connected with roof of its montant, the size of ejector pin and the size looks adaptation of putting 2 through-holes of thing frame, the outside swing joint of its horizontal pole has T form pole, the through-hole has been seted up to the inside of T form pole horizontal pole, the size of its size and the size looks adaptation of 4 horizontal poles of ejector pin, V type pole 5 is invertd to the bottom fixedly connected with of its montant.

An inverted V-shaped rod 5 is movably connected below the ejector rod 4, a groove plate is fixedly connected to the outer side of an inclined rod of the inverted V-shaped rod 5, a groove is formed in the surface of the groove plate, the inclined rod of the inverted V-shaped rod 5 is movably clamped to the outer side of the groove plate, sawteeth are fixedly connected to the outer side of the inclined rod, the size of the sawteeth is matched with that of the gear 6, the outer side of the inverted V-shaped rod 5 is movably connected with the gear 6, the outer side of the gear 6 is movably connected with a spring, one side of the spring, far away from the gear 6, is movably connected with the ejector rod 4, a first rotating plate 7 is movably connected above the gear 6, a first pull rope is movably connected to the outer side of the first rotating plate 7, the side of the first pull rope, far away from the first rotating plate 7, is movably connected with the spring, the first pull rope is movably connected to the outer side, the bump is in an inverted right-angle trapezoidal shape, an inverted Z-shaped rod 9 is movably connected below the turntable 8, an insulating rod is fixedly connected to the outer side of the inverted Z-shaped rod 9, a copper bar 10 is fixedly connected to the bottom of the insulating rod, the junction of the cross rod and the left vertical rod of the inverted Z-shaped rod 9 is movably connected with the insulating rod, and a latch is fixedly connected to the outer side of the left vertical rod.

A copper bar 10 is movably connected below the inverted Z-shaped rod 9, the copper bar 10 is electrically connected with a control center and a first rotating plate 7, the copper bar 10 is positioned between an N pole block and an S pole block of a magnetic pole group 11, the outer side of the copper bar 10 is fixedly connected with the magnetic pole group 11, the magnetic pole group 11 consists of the N pole block and the S pole block, the outer side of the magnetic pole group 11 is fixedly connected with a fixed frame, the top of the fixed frame is fixedly connected with a clamping plate, the surface of the clamping plate is provided with a notch, the size of the notch is matched with that of a clamping tooth on the outer side of a left vertical rod of the inverted Z-shaped rod 9, the outer side of the fixed frame is movably connected with a spring, one side of the spring, which is far away from the fixed frame, is movably connected with an insulating rod on the top of the copper bar 10, a through hole is arranged inside a transverse rod of the fixed frame, the size of the through hole is matched with that of the insulating rod on the, one side of the spring, which is far away from the second rotating plate 13, is movably connected with a rotating disc, one side of the rotating disc, which is far away from the second rotating plate 13, is movably connected with an inverted L-shaped rod, the outer side of a cross rod of the inverted L-shaped rod is movably connected with an elastic sheet, the inverted L-shaped rod is made of an insulating material, one side of the second rotating plate 13, which is far away from the ratchet wheel 12, is movably connected with a ring plate 14, the surface of the ring plate 14 is fixedly connected with three convex columns with the same size, the outer side of the ring plate 14 is fixedly connected with three stop blocks with the same size, inclined planes on two sides of the stop blocks are inclined, the middle area of the middle area and the stop blocks on the outer side of the ring plate 14 are positioned on the same plane, the outer.

The working process and the principle of the device are as follows:

when in use, the ratchet wheel 12 rotates, because the outer side of the ratchet wheel 12 is movably connected with a second rotating plate 13, the outer side of the second rotating plate 13 is movably connected with a spring, one side of the spring, which is far away from the second rotating plate 13, is movably connected with a rotating disc, one side of the rotating disc, which is far away from the second rotating plate 13, is movably connected with an inverted L-shaped rod, the outer side of a cross rod of the inverted L-shaped rod is movably connected with a spring sheet, one side of the second rotating plate 13, which is far away from the ratchet wheel 12, is movably connected with a ring plate 14, the surface of the ring plate 14 is fixedly connected with three convex columns with the same size, the outer side of the ring plate 14 is fixedly connected with three stop blocks with the same size, inclined planes at two sides of the stop blocks are inclined, the middle area of the stop blocks and the outer side of the ring plate 14 are positioned on the same plane, the outer side of the ring plate 14 is movably connected with a moving frame in the object placing frame 2, at the moment, the second rotating plate 13 is positioned on the inclined plane of one of the stop blocks, one convex column of the ring plate 14 is tightly attached to the outer side of the second rotating plate 13, the ring plate 14 partially winds the second pull rope on the outer side, the moving frame moves to the leftmost side under the pulling action of the second pull rope, the spring on the left side of the moving frame is extruded, the spring on the right side of the moving frame is stretched, the ratchet wheel 12 rotates and simultaneously drives the rotating plate to rotate through the inverted L-shaped rod, the rotating plate drives the second rotating plate 13 to synchronously rotate, the second rotating plate 13 moves to the middle part of the stop block along the inclined plane of the stop block, the second rotating plate 13 simultaneously extrudes the spring between the second rotating plate and the rotating disc during movement, the second rotating plate 13 no longer has the blocking effect on the convex column on the outer side, so that the moving frame in the object placing frame 2 is moved rightwards under the action of the spring on the outer side, the moving frame pulls the second pull rope, the second pull rope drives the ring plate 14 to rotate, and the subsequent second rotating plate, the ring plate 14 is driven to rotate and the second pull rope is wound, the second pull rope pulls the movable frame to move leftwards, the test tube rack 3 in the movable frame is caused to continuously shake leftwards and rightwards, and separation of cells in the test tube is promoted.

When the cell separation condition in the test tube needs to be observed, the rotating disc 8 is rotated anticlockwise, the rotating disc 8 rotates to drive a lug and a push rod on the outer side of the rotating disc, an inverted Z-shaped rod 9 is movably connected below the rotating disc 8, an insulating rod is fixedly connected on the outer side of the inverted Z-shaped rod 9, a copper rod 10 is fixedly connected at the bottom of the insulating rod, the junction of a transverse rod and a left vertical rod of the inverted Z-shaped rod 9 is movably connected with the insulating rod, a latch is fixedly connected on the outer side of the left vertical rod, the copper rod 10 is electrically connected with a control center and a first rotating plate 7, the copper rod 10 is positioned between an N pole block and an S pole block of a magnetic pole group 11, a fixed frame is fixedly connected on the outer side of the magnetic pole group 11, a snap plate is fixedly connected on the top of the fixed frame, a notch is formed in the surface of the snap plate, the size of the notch is matched with the size of the latch on the outer side, the convex block at the outer side of the rotary table 8 is close to the insulating rod at the outer side of the inverted Z-shaped rod 9, the convex block pushes the insulating rod, the inverted Z-shaped rod 9 and the copper bar 10 to move downwards, the inverted Z-shaped rod 9 moves downwards to be close to the clamping plate at the top of the fixed frame, the clamping teeth of the left vertical rod of the inverted Z-shaped rod 9 are blocked by the clamping plate in the moving process, the connecting part of the inverted Z-shaped rod 9 and the insulating rod is taken as a rotating point to rotate and be away from the clamping plate until the connecting part moves to the notch of the clamping plate, the clamping teeth of the left vertical rod of the inverted Z-shaped rod 9 rotate to be close to the clamping plate and are clamped in the notch of the clamping plate, the position of the insulating rod and the copper bar 10 after moving is fixed, the copper bar 10 moves downwards to be close to the ratchet 12, at the moment, the ratchet 12 pushes the rotary disc at the outer side of the second rotary plate 13 to rotate through the inverted L-shaped rod, the second rotating plate 13 does not rotate along with the ratchet wheel 12 any more, the ring plate 14 does not rotate back and forth any more, and the moving frame in the storage frame 2 stops vibrating left and right.

Meanwhile, the copper rod 10 cuts the magnetic field between the N pole block and the S pole block of the magnetic pole group 11 in the downward moving process to generate current, so that the control center drives the first rotating plate 7 to rotate, because the outer side of the first rotating plate 7 is movably connected with a first pull rope, one side of the first pull rope, far away from the first rotating plate 7, is movably connected with a spring, the first pull rope is movably connected with the outer side of a gear 6, the outer side of the gear 6 is movably connected with a spring, one side of the spring, far away from the gear 6, is movably connected with a top rod 4, the size of the gear 6 is matched with the size of a sawtooth at the outer side of an inclined rod of the inverted V-shaped rod 5, the outer side of the inclined rod of the inverted V-shaped rod 5 is fixedly connected with a groove plate, a groove is formed in the surface of the groove plate, the inclined rod of the inverted V-shaped rod 5 is movably clamped at the outer side, the top fixedly connected with roof of its montant, the size of roof and the size looks adaptation of putting 2 through-holes of thing frame, the roof is located the bottom of test-tube rack 3, first commentaries on classics board 7 rotates the first stay cord of releasing its outside, the spring in first stay cord outside straightens it, the process of the motion of first stay cord drives gear 6 and rotates, gear 6 and inversion V type pole 5 mesh drive its recess along the frid and move to the upper right, inversion V type pole 5 drives the T type pole synchronous motion at its top, T type pole moves and promotes ejector pin 4 and moves up along the horizontal pole right of ejector pin 4, ejector pin 4 drives the roof synchronous motion at its top, push away thing frame 2 with test-tube rack 3 part, the convenient observation to the cell in the test-tube rack 3.

When the test tube rack 3 needs to be placed into the storage frame 2 again and separated continuously, the rotary table 8 is rotated again to enable the push rod to be close to the inverted Z-shaped rod 9, the push rod pushes the right vertical rod of the inverted Z-shaped rod 9 to enable the inverted Z-shaped rod 9 to rotate by taking the joint of the inverted Z-shaped rod 9 and the insulating rod as a rotating point, the latch of the left vertical rod of the inverted Z-shaped rod 9 is far away from the notch of the latch plate at the top of the fixing frame, the insulating rod moves upwards under the action of the spring at the outer side of the insulating rod, the copper rod 10 moves upwards synchronously, the magnetic field between the S pole blocks of the N pole block of the magnetic pole group 11 is cut again, the first rotating plate 7 rotates again to cause the gear 6 to rotate reversely, the inverted V-shaped rod 5 moves towards the lower, and the copper bar 10 does not block the motion of the inverted L-shaped rod of the rotary disc at the outer side of the second rotating plate 13 any more, so that the second rotating plate 13 rotates along with the ratchet wheel 12 again, and the test tube rack 3 is caused to move left and right to vibrate.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell separation device for the biological field, comprising a housing (1), characterized in that: the utility model discloses a test tube rack, including shell (1), the outside fixedly connected with of shell (1) puts thing frame (2), the inside swing joint who puts thing frame (2) has test-tube rack (3), the bottom swing joint of test-tube rack (3) has ejector pin (4), the below swing joint of ejector pin (4) has inversion V type pole (5), the outside swing joint who inverts V type pole (5) has gear (6), the top swing joint of gear (6) has first commentaries on classics board (7), the inside swing joint of shell (1) has carousel (8), the below swing joint of carousel (8) has inversion Z type pole (9), the below swing joint who inverts Z type pole (9) has copper bar (10), the outside fixedly connected with magnetic pole group (11) of copper bar (10), the below swing joint of copper bar (10) has ratchet (12), the outside swing joint of ratchet (12) has second commentaries on classics board (13), one side that ratchet (12) were kept away from to second commentaries on classics board (13) has circle board.

2. A cell separation apparatus for use in the field of biology according to claim 1, wherein: the arc has been seted up on the surface of shell (1) and has been led to the groove, puts the bottom of thing frame (2) and seted up the through-hole, and the inside swing joint who puts thing frame (2) has the removal frame, and the inside swing joint who removes the frame has test-tube rack (3), the equal fixedly connected with cardboard in both sides of test-tube rack (3), and the mouth of placing that three size is the same is seted up to the inside of test-tube rack (3).

3. A cell separation apparatus for use in the field of biology according to claim 1, wherein: the ejector rod (4) is in an inverted L shape, the top of the vertical rod is fixedly connected with a top plate, the outer side of the transverse rod is movably connected with a T-shaped rod, a through hole is formed in the transverse rod of the T-shaped rod, and the bottom of the vertical rod is fixedly connected with an inverted V-shaped rod (5).

4. A cell separation apparatus for use in the field of biology according to claim 1, wherein: the outer side of the inclined rod of the inverted V-shaped rod (5) is fixedly connected with a groove plate, the outer side of the inclined rod is fixedly connected with sawteeth, the size of the sawteeth is matched with that of the gear (6), and the outer side of the gear (6) is movably connected with a spring.

5. A cell separation apparatus for use in the field of biology according to claim 1, wherein: the outer side of the first rotating plate (7) is movably connected with a first pull rope, one side, away from the first rotating plate (7), of the first pull rope is movably connected with a spring, the first pull rope is movably connected to the outer side of the gear (6), the outer side of the rotary plate (8) is fixedly connected with a shifting tooth, and the outer side of the rotary plate (8) is fixedly connected with a convex block and a push rod.

6. A cell separation apparatus for use in the field of biology according to claim 1, wherein: the outside fixedly connected with insulator spindle of inversion Z type pole (9), the bottom fixedly connected with bar copper (10) of insulator spindle, magnetic pole group (11) comprise N utmost point piece and S utmost point piece, and the fixed frame of outside fixedly connected with of magnetic pole group (11), the top fixedly connected with cardboard of fixed frame, the outside swing joint of fixed frame has the spring.

7. A cell separation apparatus for use in the field of biology according to claim 1, wherein: the outside swing joint spring of second commentaries on classics board (13), the spring is kept away from second commentaries on classics board (13) have one side swing joint to have the capstan, one side swing joint that the second commentaries on classics board (13) was kept away from to the capstan has the L type pole of inversion, the surperficial fixedly connected with three equal-size projection of circle board (14), the three equal-size dog of outside fixedly connected with of circle board (14), the outside swing joint of circle board (14) has the second stay cord, one side that circle board (14) were kept away from to the second stay cord with put the removal frame swing joint in thing frame (2).