CN111789127A

CN111789127A - Insecticidal composition containing indoxacarb and cyclic bromoantraniliprole and application thereof

Info

Publication number: CN111789127A
Application number: CN202010720268.8A
Authority: CN
Inventors: 张方贺; 刘坤; 邓晓杰; 乔志强; 许兴志; 成道泉; 王成
Original assignee: Jingbo Agrochemicals Technology Co Ltd
Current assignee: Jingbo Agrochemicals Technology Co Ltd
Priority date: 2020-07-23
Filing date: 2020-07-23
Publication date: 2020-10-20

Abstract

The invention belongs to the field of pesticides, and provides an insecticidal composition containing indoxacarb and cyclic bromoantraniliprole and application thereof. The composition contains indoxacarb and cyclic bromoantraniliprole, wherein the weight ratio of the indoxacarb to the cyclic bromoantramide is 50: 1-1: 50. the composition can be used for preventing and treating agricultural insect pest in cultivated land, mountain land, and protected land. Especially for the prevention and treatment of various lepidoptera pests such as diamondback moth, rice leaf roller, black cutworm, cotton bollworm, cabbage caterpillar, beet armyworm, inchworm and the like. The composition has wide application range, good effect, low cost, low residue, and safety to human, livestock, and environment.

Description

Insecticidal composition containing indoxacarb and cyclic bromoantraniliprole and application thereof

Technical Field

The invention belongs to the technical field of pesticides, and provides an insecticidal composition containing indoxacarb and cyromonamide and application thereof.

Background

At present, for pest control which is easy to generate resistance in agriculture, the pesticide species with different action mechanisms are the best choice for mixing. If the proportion is reasonable, the composition can generate obvious synergistic action, so that the control effect is obviously better than that of each single agent. The pesticide containing a single active ingredient has certain defects in agricultural disease control, continuous and repeated use not only enables pests to easily generate drug resistance, but also easily causes pollution to food and environment, and the defects can be overcome by reasonably mixing the active ingredients of the pesticide. The reasonable compounding ensures that the effective components generate synergistic action, can improve the control effect, reduce the using amount of the effective components, save the cost, delay the generation of drug resistance of pests, and further can reduce or even avoid the pollution of pesticides to food and environment.

Indoxacarb (indoxacarb), formula: s-7-chloro-2, 5-dihydro-2- [ [ (methoxycarbonyl) [4- (trifluoromethoxy) -phenyl ] amino ] carbonyl ] indeno [1, 2-e ] [1, 3, 4] oxadiazine-4 (3H) -carboxylic acid methyl ester with the chemical formula as follows:

indoxacarb has the effects of contact killing and stomach toxicity, and has obvious effect on larvae at all ages. The pesticide enters the insect body by contacting and feeding, the insect stops feeding within 0-4h, and falls off from crops due to paralysis and reduced coordination ability, generally paralyzes and kills within 4-48h after the pesticide is applied, and is effective on larvae of all ages. The pests can have a period of time from the contact with the liquid medicine or the eating of the leaves containing the liquid medicine to the death of the pests, but the pests stop eating the crops at the moment, and even if the pests do not die at the moment, the leaves or the buds of the crops are not damaged for a month. The indoxacarb has better lipophilicity, only has the effects of contact poisoning and stomach poisoning, and has better rain wash resistance although the indoxacarb has no systemic activity. The test result shows that the indoxacarb has no cross resistance with other insecticides such as pyrethrins, organic phosphorus, carbamates and the like. Is safe to fishes, mammals and natural enemy insects including mites, and is therefore an ideal agent for comprehensive control and resistance control of pests.

Cyclic bromodiamide (cyclaniliprole), formula: 2, 3-dibromo-4-chloro-1- (3-chloro-2-pyridinyl) -6- { [ (1RS) -1-cyclopropylethyl ] carbamoyl } pyrazole-5-carboxanilide of formula (la):

the cyclic bromoantraniliprole (cyclobromoantrole) is a novel bisamide insecticide, has broad-spectrum insecticidal activity, can be used for numerous crops such as fruit trees, vegetables, potatoes, tea trees, soybeans, cotton and the like, and can be used for preventing and treating lepidoptera, coleoptera, thysanoptera, diptera and homoptera pests, is high-efficiency for target pests, and is safe for non-target crops. The main action site is a ryanodine receptor variant, and the main action site is different from other bisamide insecticides, and the agent is not completely a ryanodine receptor agent. At present, no report related to the use of indoxacarb and cyromonamide is found.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides an insecticidal composition containing indoxacarb and cyromonamid and application thereof. The composition contains indoxacarb and cyclic bromoantraniliprole, wherein the weight ratio of the indoxacarb to the cyclic bromoantramide is 50: 1-1: 50. the composition can be used for preventing and treating agricultural insect pest in cultivated land, mountain land, and protected land. Especially for the prevention and treatment of various lepidoptera pests such as diamondback moth, rice leaf roller, black cutworm, cotton bollworm, cabbage caterpillar, beet armyworm, inchworm and the like. The composition has wide application range, good effect, low cost, low residue, and safety to human, livestock, and environment.

In the prior art, a technical scheme of compounding the annular chlorfenapyr and the chlorfenapyr serving as main active components appears, although the chlorfenapyr and the indoxacarb in the application have similarities in an insecticidal mechanism, the inventor finds that compared with the chlorfenapyr, the lasting period of the indoxacarb is higher than that of the chlorfenapyr through long-term test, and the application times can be reduced; the indoxacarb is capable of killing lepidoptera pests in a large and small mode, the chlorfenapyr only has an obvious effect on the large-age pests, and the application range of the indoxacarb is wider; and meanwhile, the safety of the indoxacarb is higher than that of the chlorfenapyr, so that the pesticide injury is avoided, the pesticide application risk can be reduced, and the body health of a user is protected.

The inventor utilizes the synergistic effect generated by mixing the two active components of indoxacarb and the cyclic bromoantraniliprole, can improve the control effect, reduce the dosage of active ingredients, save the medication cost, delay the generation of drug resistance of pests, reduce the environmental pollution and comprehensively control agricultural diseases; the inventor develops the low-toxicity high-efficiency pesticide proportion by screening a large amount of pesticide formulas and researching on the prior basis, not only obviously enhances the pesticide effect on a target, but also has low influence on human, crops, non-targets and the environment. By compounding indoxacarb and the cyclic bromoantraniliprole, the drug resistance can be delayed, and the synergistic effect is obvious.

The specific technical scheme of the invention is as follows:

an insecticidal composition containing indoxacarb and cyromanilide, wherein the active ingredients of the composition are indoxacarb and cyromanilide, and the weight ratio of the indoxacarb to the cyromanilide is 50: 1-1: 50, and the weight sum of the indoxacarb and the cyclic bromoantraniliprole accounts for 2-80% of the total weight of the agricultural sterilization compound composition.

The composition is a common preparation in the field such as missible oil, water dispersible granules, wettable powder, suspending agent, microemulsion, aqueous emulsion, film-spreading oil agent, sustained-release granules and the like, and the balance of the composition is a common auxiliary agent in pesticide production such as acceptable solvent, auxiliary agent or filler and the like in pesticide production besides the active components.

Further, the weight ratio of the indoxacarb to the cyclic bromoantraniliprole is 1-20: 1-20;

furthermore, the weight ratio of the indoxacarb to the cyclic bromoantraniliprole is 10: 1-1: 10;

most preferably, the weight ratio of the indoxacarb to the cyclic bromoxynil is 1: 5.

on the basis of the technical scheme, the inventor further supplements various auxiliary agents as follows:

mainly comprises an emulsifier, a wetting agent, a dispersant, a disintegrant, a thickening agent, a stabilizer, an antifreezing agent and a defoaming agent;

wherein the emulsifier mainly comprises: alkyl polyoxyethylene ether, alkyl phenol polyoxyethylene polyoxypropylene ether, fatty amine, ethylene oxide adduct of fatty amide and the like, fatty acid polyoxyethylene ether, sodium dodecylbenzene sulfonate, alkyl (phenol) polyoxyethylene polyoxypropylene ether, calcium dodecylbenzene sulfonate, an alkanol fatty acid modifier, and sodium lauryl sulfate, in any proportion, but the invention is not particularly limited.

The wetting agent mainly comprises: one or a combination of several of alkyl sulfonic acid sodium salt, nekal and saponin powder in any proportion, but the invention is not limited specifically.

The dispersant mainly comprises: one or a combination of several of sulfonates, sulfates, polycarboxylates, phosphates, polyoxyethylene ethers, and lignin wetting agents in any proportion, but the present invention is not particularly limited.

The disintegrating agent mainly comprises: one or a combination of several of ammonium sulfate, sodium carbonate and potassium carbonate in any proportion, but the invention is not particularly limited.

The thickener mainly includes sodium carboxymethylcellulose, xanthan gum, magnesium aluminum silicate, guar gum, etc., but is not particularly limited to the present invention.

The stabilizer or antifreeze mainly comprises one or a combination of more of sorbitol, isopropanol, glycol, glycerol, polyvinyl alcohol and urea in any proportion, but is not particularly limited in the invention.

The crosslinking agent mainly includes chloroacetic acid, maleic anhydride-styrene copolymer, etc., but is not particularly limited to the present invention.

The defoaming agent mainly includes silicone and the like, but is not particularly limited to the present invention.

The solvent is mainly DMF, xylene, solvent oil, cyclohexanol, castor oil, water, epoxidized soybean oil;

the filler is mainly auxiliary agents, solvents and fillers acceptable for various pesticide formulations such as kaolin, light calcium carbonate, bentonite, white carbon black, sodium bentonite and the like.

Further on the basis of the technical scheme, the inventor provides the following specific technical scheme of the corresponding dosage form:

the indoxacarb and cyclic bromoantraniliprole emulsion formulation composition is prepared by dissolving and uniformly stirring indoxacarb, cyclic bromoantraniliprole, a solvent and an emulsifier.

Wherein the weight percentages of the components are as follows:

the indoxacarb and cyclic bromoantraniliprole water dispersible granule composition is prepared by mixing, crushing, extruding and granulating indoxacarb, cyclic bromoantraniliprole and proper wetting agent, dispersing agent and disintegrating agent.

Wherein the weight percentages of the components are as follows:

indoxacarb	1％-50％
		Cyclic bromodiamide	1％-50％
Wetting agent	1％-30％
		Dispersing agent	1％-30％
Disintegrating agent	1％-10％
		Filler material	Balance of

The indoxacarb and cyclic bromoantraniliprole wettable powder composition is prepared by mixing, coarsely crushing and airflow-crushing indoxacarb, cyclic bromoantraniliprole, a proper wetting agent, a proper dispersing agent and a proper filler.

Wherein the weight percentages of the components are as follows:

indoxacarb	1％-50％
		Cyclic bromodiamide	1％-50％
Wetting agent	1％-30％
		Dispersing agent	1％-30％
Filler material	Balance of

The indoxacarb and cyclic bromoantraniliprole suspending agent composition is prepared by pre-crushing indoxacarb and cyclic bromoantraniliprole, as well as a proper wetting agent, a proper dispersing agent, a proper emulsifying agent and water, adding the crushed indoxacarb and cyclic bromoantraniliprole into a sand mill for grinding, adding a proper proportion of a thickening agent, a proper emulsifying agent, a proper defoaming agent, a proper proportion of an antifreezing agent and the like, and shearing and mixing at a high speed.

Wherein the weight percentages of the components are as follows:

indoxacarb	1％-50％
		Cyclic bromodiamide	1％-50％
Wetting agent	1％-30％
		Dispersing agent	1％-30％
Emulsifier	1％-30％
		Thickening agent	0.1％-1％
Antifreezing agent	1％-3％
		Defoaming agent	0.1％-1％
Water (W)	Balance of

The indoxacarb and cyclic bromoantraniliprole microemulsion composition is prepared by dissolving indoxacarb, cyclic bromoantraniliprole and an emulsifier into a uniform oil phase; mixing the oil phase and the water phase under stirring.

Wherein the weight percentages of the components are as follows:

indoxacarb	1％-5％
		Cyclic bromodiamide	1％-5％
Solvent(s)	10％-40％
		Emulsifier	1％-30％
Water (W)	Balance of

The indoxacarb and cyclic bromoantraniliprole aqueous emulsion composition is a uniform oil phase which is formed by dissolving indoxacarb, cyclic bromoantraniliprole, a solvent and an emulsifier; under high-speed stirring, the oil phase and the water phase are subjected to high-shear homogeneous mixing to obtain the oil-water phase emulsion.

Wherein the weight percentages of the components are as follows:

indoxacarb	1％-10％
		Cyclic bromodiamide	1％-10％
Solvent(s)	10％-30％
		Emulsifier	1％-30％
Water (W)	Balance of

The indoxacarb and cyclic bromoantraniliprole spreading oil agent composition is prepared by dissolving and uniformly stirring indoxacarb, cyclic bromoantraniliprole, a solvent and an emulsifier.

Wherein the weight percentages of the components are as follows:

indoxacarb	1％-5％
		Cyclic bromodiamide	1％-5％
Solvent(s)	Balance of
		Emulsifier	1％-30％

Indoxacarb and cyclic bromoantraniliprole sustained-release granule composition is prepared by mixing a cross-linking agent, part of filler and water, heating for 60 minutes, adding indoxacarb, cyclic bromoantraniliprole and a dispersing agent, mixing for 5 minutes at 50 ℃ at 2000 rpm, solidifying for 5 hours at 30 ℃, drying, crushing, adding the rest filler and a stabilizing agent, granulating by a disc granulator at the rotating speed of 60 rpm, and obtaining particles with required particle size by a grading sieve.

Wherein the weight percentages of the components are as follows:

indoxacarb	1％-50％
		Cyclic bromodiamide	1％-50％
Crosslinking agent	1％-30％
		Dispersing agent	1％-30％
Stabilizer	1％-4％
		Filler material	Balance of

The insecticidal composition containing indoxacarb and cyclic bromoantraniliprole provided by the invention has higher control effect on agricultural insect pests in farmlands, mountainous regions and protected areas, and mainly on lepidoptera pests such as rice leaf rollers, cotton bollworms, plutella xylostella and beet armyworms.

The invention has the following beneficial effects:

1. the indoxacarb and the cyromonamid in the composition belong to insecticides with different action mechanisms, a single active ingredient has extremely high resistance risk after being used for a long time, the indoxacarb and the cyromonamid are compounded and used to achieve medicament complementation, the service life of each single agent is greatly prolonged, the effect of delaying the generation of pest resistance is realized, and the composition has important significance for overall management of the resistance problem of the medicaments.

2. The invention has the characteristics of high efficiency and environmental pollution reduction, shows obvious synergistic action in a certain proportioning range, obviously improves the control effect compared with a single agent, reduces the using dosage of pesticide, simultaneously reduces the pesticide cost, and greatly reduces the adverse effect on the environment.

3. The agricultural insecticidal compound composition provided by the invention has higher control effect on preventing and controlling lepidoptera pests such as rice leaf roller, cotton bollworm, diamond back moth, beet armyworm and the like.

Detailed Description

The present invention will be described in further detail with reference to the following examples, but it should not be construed that the scope of the above subject matter is limited to the following examples. All the technologies realized based on the above contents of the invention belong to the scope of the invention, the following adopted additives are all conventional additives in the field, and the proportions shown in the examples are all weight percentages.

Example 1

30% indoxacarb-cyclobromantraniliprole water dispersible granule (preparation 1, indoxacarb: cyclobromantraniliprole 1: 1)

15% of indoxacarb, 15% of cyclic bromodiamide, 3.5% of polycarboxylate, 9.6% of calcium lignosulfonate, 5% of naphthalenesulfonate dispersant, 3% of nekal and ammonium sulfate to be supplemented to 100%. The first formula is prepared into 30% indoxacarb-cyclobromantraniliprole water dispersible granules by dry crushing, kneading, extruding, granulating, drying and screening according to a proportion.

Example 2:

51% indoxacarb-cyclobromantraniliprole water dispersible granule (preparation 2, indoxacarb: cyclobromantraniliprole 1: 50)

1% of indoxacarb, 50% of cyclic bromodiamide, 5% of polycarboxylate, 5% of naphthalenesulfonate dispersant, 3% of saponin powder and 100% of ammonium sulfate. The first formula is prepared into 51% indoxacarb-cyclobromantraniliprole water dispersible granules by dry crushing, kneading, extruding, granulating, drying and screening according to a proportion.

Example 3:

31% indoxacarb-cyclobromantraniliprole wettable powder (preparation 3, indoxacarb: cyclobromantraniliprole ═ 1: 30)

1% of indoxacarb, 30% of cyclic bromantraniliprole, 5% of naphthalenesulfonate dispersant, 7% of polycarboxylate, 8% of white carbon black and 100% of kaolin. The components are coarsely crushed according to a certain proportion, then are put into a mixer to be mixed uniformly, and are crushed by air flow to prepare the 31 percent indoxacarb-cyclobromantraniliprole wettable powder.

Example 4:

22% indoxacarb-cyclobromantraniliprole suspension (formulation 4, indoxacarb: cyclobromantraniliprole 1: 10)

2% of indoxacarb, 20% of cyclic bromodiamide, 8% of benzyl polyoxyethylene ether, 1602# agricultural emulsion, 7% of distyrylphenol polyoxyethylene polyoxypropylene ether, 0.15% of xanthan gum, 2% of organic silicon, 2% of ethylene glycol and the balance of water. The materials are sheared and mixed in advance according to the formula, and then the mixture is added into a sand mill for grinding to prepare the 22% indoxacarb-cyclic bromodiamide suspending agent after the grinding.

Example 5:

24% indoxacarb-cyclobromantraniliprole suspension (formulation 5, indoxacarb: cyclobromantraniliprole 1: 5)

4% of indoxacarb, 20% of cyclic bromoxynil amide, 8% of benzyl polyoxyethylene ether, 1602# agricultural emulsion, 7% of distyrylphenol polyoxyethylene polyoxypropylene ether, 0.15% of xanthan gum, 2% of organic silicon, 2% of ethylene glycol and the balance of water. The materials are sheared and mixed in advance according to the formula, then the mixture is added into a sand mill for grinding, and the 24% indoxacarb-cyclic bromodiamide suspending agent is prepared after the grinding.

Example 6:

6% indoxacarb-cyclic bromantraniliprole microemulsion (preparation 6, indoxacarb: cyclic bromantraniliprole 1: 2)

2% of indoxacarb, 4% of cyromonamide, 8% of xylene, 20% of ethyl acetate, 6% of Nongru 500, 5% of Nongru 602, 2% of Nongru 1601, 5% of ethylene glycol and the balance of water to 100%. Mixing the raw materials, a solvent and an emulsifier in proportion to dissolve the raw materials into a uniform oil phase; mixing ethylene glycol and water to prepare a water phase; mixing the oil phase and the water phase under stirring to obtain the 6% indoxacarb-cyclic bromantraniliprole microemulsion.

Example 7:

9% indoxacarb-cyclic bromantraniliprole emulsion in water (preparation 7, indoxacarb: cyclic bromantraniliprole 2: 1)

6% of indoxacarb, 3% of cyromonamide, 8% of xylene, 20% of ethyl acetate, 10% of cyclohexanol, 5% of glycerol, 6% of Nongru 500, 5% of Nongru 602 and water to make up to 100%. Dissolving the raw materials, the solvent and the emulsifier in the formula into a uniform oil phase; mixing glycerol and deionized water in the formula to prepare a water phase; under high-speed stirring, the oil phase and the water phase are subjected to high shear and homogeneous mixing to prepare 9% indoxacarb-cyclobromantraniliprole emulsion in water.

Example 8:

6% indoxacarb-cyclic bromantraniliprole sustained-release granules (preparation 8, indoxacarb: cyclic bromantraniliprole 5: 1)

5% of indoxacarb, 1% of cyclic bromodiamide, 5% of polyvinyl alcohol, 2% of chloroacetic acid, 3% of white carbon black, 2% of polyoxyethylene styryl phenyl ether, 4% of water and the balance of clay to 100%. Mixing and heating chloroacetic acid, white carbon black and water for 60 minutes, adding indoxacarb, cyclic bromoantraniliprole and polyoxyethylene styryl phenyl ether, mixing at 50 ℃ for 5 minutes at 2000 r/min, solidifying at 30 ℃ for 5 hours, drying, crushing, adding clay and polyvinyl alcohol, granulating by a disc granulator at the rotating speed of 60 r/min, and obtaining particles with required particle size by a grading sieve to obtain the 6% indoxacarb-cyclic bromoantraniliprole sustained-release granules.

Example 9:

5.5% indoxacarb-cyclic bromantraniliprole spreading oil agent (preparation 9, indoxacarb: cyclic bromantraniliprole 10: 1)

5% of indoxacarb, 0.5% of cyclic bromoantraniliprole, 70.5% of xylene, 10% of ethyl acetate, 5% of Nongru No. 603 and 9% of isopropanol. Uniformly mixing dimethylbenzene, ethyl acetate and isopropanol, then adding indoxacarb and cyclobromantraniliprole, then adding Nongru No. 603, and uniformly stirring to obtain the 5.5% indoxacarb-cyclobromantramide spreading oil agent.

Example 10:

31% indoxacarb-cyclobromantraniliprole water dispersible granule (preparation 10, indoxacarb: cyclobromantraniliprole 30: 1)

30% of indoxacarb, 1% of cyclic bromoantraniliprole, 4% of sodium dodecyl benzene sulfonate, 5% of calcium lignosulphonate, 5% of polycarboxylate, 3% of ammonium sulfate and 100% of light calcium carbonate. The formula is prepared into the 31 percent indoxacarb-cyclobromantraniliprole water dispersible granule by dry crushing, kneading, extruding granulation, drying and screening according to the proportion.

Example 11:

51% indoxacarb-cyclobromantraniliprole wettable powder (preparation 11, indoxacarb: cyclobromantraniliprole 50: 1)

50% of indoxacarb, 1% of cyclic bromoantraniliprole, 4% of lauryl alcohol-based sodium sulfate, 7% of alkyl sodium sulfonate, 8% of white carbon black and 100% of kaolin. The components are coarsely crushed according to a certain proportion, then are put into a mixer to be mixed uniformly, and are crushed by air flow to prepare the 51 percent indoxacarb-cyclobromantraniliprole wettable powder.

Test example 1:

indoor bioassay tests are carried out to verify the pesticide effect tests of the indoxacarb and the cyclic bromoantraniliprole for preventing and treating the agrotis ypsilon.

Experimental agents: examples 1-11 formulations, comparative example 15% indoxacarb suspension, 30% cyromonamide.

The test method comprises the following steps: randomly placing the eupatorium parvifolium which is normal in development, consistent in size and same in age into culture dishes with filter paper, placing the small leaf dishes soaked in the liquid medicine into the culture dishes with the eupatorium parvifolium, placing the blank control group into the small leaf dishes which are not added with the medicine, placing 3 small leaf dishes in each dish, placing absorbent paper into the culture dishes, adding water into the culture dishes, moisturizing, placing the culture dishes into an electric heating constant-temperature air-blowing drying oven at 26 +/-1 ℃, with the relative humidity of 80 +/-5 percent and under the illumination condition of L: D: 14h:10h, observing and recording the death number of the test insects after 96h, and calculating the insecticidal activity.

Insecticidal activity (%) - (ck live insect number-number of active insects for medicament)/ck live insect number 100%

Pesticidal activity of test agent

Processing number	Treatment of	The effective component is used per hectare/g	Insecticidal Activity/%
				1	Example 1	56	85
2	Example 2	56	85
				3	Example 3	56	84
4	Example 4	56	84
				5	Example 5	56	88
6	Example 6	56	87
				7	Example 7	56	88
8	Example 8	56	81
				9	Example 9	56	80
10	Example 10	56	82
				11	Example 11	56	77
12	15% indoxacarb SC	56	70
				13	30% Cyclobromantraniliprole SC	56	65
14	Control CK	--	--

Indoor bioassay tests prove that the insecticidal activity of the agricultural composition of the indoxacarb and the cyclic bromoantraniliprole on the agrotis ypsilon is greater than that of the agricultural composition of the indoxacarb and the cyclic bromoantraniliprole when the two medicines are used independently under the same use amount. The two medicament compositions have the effect of additive synergism, and the optimal ratio of the two compounds is further determined by indoor toxicity detection.

Test example 2: indoxacarb and cyclic bromoantraniliprole composition indoor toxicity test

Measuring indoor toxicity, wherein the test medicament adopts 97% indoxacarb technical and 95% cyclic bromantraniliprole technical; the test target is the black cutworm, and the consistent 3 rd larva is selected for the test.

The test method comprises the following steps: dissolving and diluting a reagent to be tested by using acetone by adopting a leaf soaking method, beating clean cabbage leaves into a wafer with the diameter of about 2cm by using a puncher, soaking the leaves into corresponding reagent treatment concentration, taking out the leaves after 10 seconds, naturally drying the leaves, and placing the leaves in a 24-hole culture dish. Randomly inoculating 3-year-old larvae of Gekko parvus which have been starved for 4h, treating 24 larvae at each concentration, and repeating for 4 times. The control was treated with clean water. The culture dish is placed in an artificial climate box with the temperature of 25 +/-1 ℃ and the relative humidity of 75 percent and the ratio of 16L to 8D, the survival condition of 3-instar larvae is checked after 48 hours, and the death standard is that no obvious autonomous reaction occurs when the fine hairbrush touches the larva body. If the mortality rate of the control group is more than or equal to 10 percent, the test needs to be carried out again.

The joint mode of action was determined using the co-toxicity coefficient (CTC) method. The co-toxicity coefficient is calculated as follows: the virulence index (ATI) was measured as (standard agent LC 50/test agent LC50) × 100.

Theoretical virulence index (TTI) ═ a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture.

Co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].

According to NY/T11547.7-2006 pesticide combination division standard: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80 < co-toxicity coefficient (CTC) < 120 showed additive effects. The test results are shown in Table 1.

TABLE 2 Indanxianwei and Cyclobromantraniliprole indoor joint virulence determination

As can be seen from the table 2, the composition disclosed by the embodiment of the invention has very high activity on black cutworm, and the co-toxicity coefficients of the indoxacarb and the cyclic bromoantraniliprole are both more than 120 within the ratio of 5: 1-1: 30, which shows that the compound pesticide has a synergistic effect; the mass ratio is 1: 1-1: 5, the co-toxicity coefficients are all over 140, the synergistic effect is more obvious, and particularly, the mass ratio is 1: the synergistic effect is most obvious when 5, so that the combination of the indoxacarb and the cyclic bromoantraniliprole has reasonability and feasibility.

Test example 3: pesticide effect test for preventing and controlling diamondback moth in field test

The test plot is flat, the management of fertilizer and water is good, and the pesticide is applied in the full-blown period of the young diamondback moth larvae. Experiments show that 24% of indoxacarb-cyclobromoantraniliprole SC active ingredient is 56 g/hectare, a contrast agent is 15% of indoxacarb SC active ingredient is 56 g/hectare, 30% of cyclobromoantraniliprole SC active ingredient is 56 g/hectare, 15% of cyclobromoantraniliprole water dispersible granule active ingredient is 56 g/hectare and clear water is contrasted for 5 treatments, the area of a cell is 20m²4 replicates, randomized block permutation. The spraying liquid is sprayed by a knapsack manual sprayer in a conventional way, and the spraying liquid amount is 675kg/hm². And (3) adopting a fixed-point fixed-plant investigation method to investigate the number of larvae on the 15 cabbage plants at fixed points in each cell. Investigating population base before applying pesticide, investigating residual live insect number on cabbage 7d, 15d and 21d after pesticide application, and calculating the control effect of each treatment according to population reduction rate.

The calculation formula is as follows:

the reduction rate of worm mouth is (base of worm mouth before medicine-base of worm mouth after medicine) ÷ base of worm mouth before medicine × 100%

The correction control effect is (the population reduction rate of the treated group-the population reduction rate of the control group) ÷ (1-the population reduction rate of the control group) × 100%

TABLE 3 field efficacy control results for diamondback moth with different treatments

The test results in table 3 show that the control effect of the pesticide applied to the plutella xylostella is significantly higher than that of the control pesticide, and the control effect of the pesticide applied to the plutella xylostella is still over 90% 25 days after the pesticide is applied, which shows that the indoxacarb and the cyclic bromoxynil compound pesticide have obvious synergistic effect, and the lasting time is obviously longer than that of a single pesticide. Experiments show that the compound pesticide obviously reduces the using amount of the pesticide, and reduces the production cost, pesticide residue and environmental pollution. Therefore, the indoxacarb and the cyromanil are ideal compound insecticidal products, and have obvious improvement compared with the combination of the cyromanil and the chlorfenapyr.

Claims

1. An insecticidal composition containing indoxacarb and cyclic bromoantraniliprole, which is characterized in that: the active ingredients of the composition are indoxacarb and cyclic bromoantraniliprole, and the weight ratio of the indoxacarb to the cyclic bromoantraniliprole is 50: 1-1: 50, and the weight sum of the indoxacarb and the cyclic bromoantraniliprole accounts for 2-80% of the total weight of the agricultural sterilization compound composition.

2. An insecticidal composition according to claim 1, wherein the weight ratio of indoxacarb to cyromonamide is 1-20: 1 to 20.

3. An insecticidal composition according to claim 1 or 2, wherein the weight ratio of indoxacarb to cyromaniliprole is 10: 1-1: 10.

4. an insecticidal composition according to claim 1, wherein the weight ratio of indoxacarb to cyromaniliprole is 1: 5.

5. the insecticidal composition according to claim 1, wherein the composition is in the form of one of emulsifiable solution, water dispersible granules, wettable powder, suspending agent, microemulsion, aqueous emulsion, spreading oil and slow release granules.

6. Use of the insecticidal composition according to any one of claims 1 to 5 for controlling agricultural pests on cultivated lands, mountainous areas, protected areas.