CN111426733B - Electrode material for ascorbic acid sensor, biosensor, preparation method and application of biosensor - Google Patents
Electrode material for ascorbic acid sensor, biosensor, preparation method and application of biosensor Download PDFInfo
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Abstract
The invention relates to the technical field of biosensors, in particular to an electrode material for an ascorbic acid sensor, the biosensor, a preparation method and an application thereof, wherein the electrode material is prepared by the following steps: the method comprises the steps of carrying out surface oxidation on foamed nickel subjected to surface deoiling treatment through a hydrothermal oxidation method to prepare a Ni @ NiO composite material, modifying the surface of the Ni @ NiO composite material through an electro-spraying method by using an oxidized carbon nano tube to obtain a Ni @ NiO composite material/carbon nano tube, and loading ascorbic acid oxidase on the surface of the Ni @ NiO composite material/carbon nano tube to prepare the electrode material for detecting ascorbic acid. The electrode material, the reference electrode and the counter electrode form a biosensor, ascorbic acid can be detected by adopting an electrochemical method, and the ascorbic acid detection has high sensitivity, low detection limit, quick response time and high selectivity.
Description
Technical Field
The invention relates to the technical field of biosensors, in particular to an electrode material for an ascorbic acid sensor, the biosensor, a preparation method and an application thereof.
Background
The sensor (english name: transducer/sensor) is a detection device, which can sense the measured information and convert the sensed information into electric signals or other information in required form according to a certain rule to output, so as to meet the requirements of information transmission, processing, storage, display, recording, control and the like.
Ascorbic acid (vitamin C) is ingested by food, absorbed by the small intestine, widely distributed in internal organs and tissues, and biochemically participates in collagen synthesis, l-carnitine synthesis, adrenocortical hormone synthesis, catecholamine synthesis, lipid peroxide decomposition, active oxygen decomposition, and the like, and plays an important role in the living body. In recent years, attention has been paid to anticancer effects, immunity enhancement, and skin-beautifying and whitening effects due to the strong reducibility of vitamin C, and the vitamin C is used in high-concentration vitamin C spot therapy and the like.
Examples of the measurement of ascorbic acid include measurement for diagnosing ascorbic acid deficiency, measurement of ascorbic acid in food, and the like. In addition, in the high-concentration vitamin C spot therapy, it is necessary to monitor the vitamin C concentration in blood immediately, and in the self-blood glucose measurement of diabetes, the glucose value is affected by ascorbic acid in blood, and therefore, it is necessary to correct the glucose value based on the measurement value of the ascorbic acid concentration.
Therefore, the determination of ascorbic acid is always an important index for detecting the health of a human body medically, and the determination of ascorbic acid generally adopts a titration analysis method, a spectrophotometry method, a chromatographic analysis method and a fluorescence analysis method, but the methods in the prior art require higher experimental conditions and operation techniques and have lower detection sensitivity.
Disclosure of Invention
In view of the technical defects, the invention aims to provide an electrode material for an ascorbic acid sensor, a biosensor, a preparation method and an application thereof.
In order to solve the technical problems, the invention adopts the following technical scheme:
an electrode material for detecting ascorbic acid, which is prepared by the following method: firstly, carrying out surface oxidation on foamed nickel subjected to surface deoiling treatment through an oxidation method to prepare a Ni @ NiO composite material, then modifying the oxidized carbon nanotube on the surface of the Ni @ NiO composite material through an electro-spraying method to obtain a Ni @ NiO composite material/carbon nanotube, and finally loading ascorbic acid oxidase on the surface of the Ni @ NiO composite material/carbon nanotube to prepare the electrode material for detecting ascorbic acid.
The invention also provides a preparation method of the electrode material for detecting the ascorbic acid, which comprises the following steps:
(1) Preparation of Ni @ NiO composite:
after the surface of the foamed nickel is subjected to deoiling treatment, surface oxidation is carried out through an oxidation method, and the Ni @ NiO composite material is prepared;
(2) Preparation of electrode material for detecting ascorbic acid: transferring the carbon oxide nanotube prepared by a chemical oxidation method to the surface of a Ni @ NiO composite material by adopting an electronic injection method, carrying out heat treatment to obtain a Ni @ NiO composite material/carbon nanotube, and fixing ascorbic acid oxidase on the surface of the Ni @ NiO composite material/carbon nanotube to prepare the electrode material for detecting ascorbic acid.
Preferably, the surface degreasing method in the step (1) comprises the following steps: soaking foamed nickel in acetone and performing ultrasonic treatment, then performing ultrasonic treatment in deionized water, soaking the foamed nickel in HCl solution with the concentration of 0.1mol/L and performing ultrasonic treatment, then performing ultrasonic treatment in deionized water, and drying at normal temperature until the quality does not change any more.
Preferably, the hydrothermal oxidation method in step (1) is: putting the foamed nickel and deionized water into a reaction kettle, reacting for 12 hours at 100-120 ℃ in the reaction kettle, then reacting for 12 hours at 130-140 ℃, cooling to room temperature, taking out and drying, finally sintering for 5-7 hours at 300-400 ℃, and drying in vacuum.
Preferably, the preparation method of the chemical oxidation method of the carbon nanotube in the step (2) comprises: adding concentrated nitric acid with volume fraction of 45% into carbon nano tube, refluxing at 100-120 deg.C for 10-12h, cooling to room temperature, washing with deionized water until deionized water is colorless and transparent, and oven drying until the mass is not changed.
Preferably, the step (2) of immobilizing the ascorbate oxidase on the surface of the ni @ nio composite/carbon nanotube comprises: dripping ascorbic acid oxidase solution on the Ni @ NiO composite material/carbon nano tube repeatedly for 3-5 times, drying at normal temperature, and refrigerating at low temperature;
the preparation method of the ascorbic acid oxidase solution comprises the following steps: 500 units ascorbic acid oxidase is dissolved in 100 mul of chitosan acetic acid solution with the mass percent concentration of 1 percent.
Preferably, the specific operation of the electrospray method in the step (2) is as follows: ultrasonically dispersing carbon oxide nanotubes in N, N' -dimethylformamide or N-methylpyrrolidone to form dispersion liquid, injecting the dispersion liquid into an injection container with a spray head, and injecting the dispersion liquid in the injection container to the surface of the Ni @ NiO composite material through the spray head after electrifying;
the electro-spraying method adopts the following spraying conditions: the temperature is 80-120 ℃, the distance between the surface of the Ni @ NiO composite material and the spray head is 10-14cm, the spray flow is 0.05-0.1mL/h, the voltage is 8-12kV, and the inner diameter of the spray head is 0.4-0.6mm.
Preferably, the heat treatment operation in the step (2) is: treating at 300-450 deg.C for 10-20min in inert atmosphere.
The invention also protects a biosensor of an electrode material for detecting ascorbic acid, which comprises the electrode material for detecting ascorbic acid, a reference electrode, an auxiliary electrode and electrolyte, wherein the electrode material for detecting ascorbic acid, the reference electrode, the auxiliary electrode and the electrolyte are prepared in the step (2), the electrode material for detecting ascorbic acid is used as a working electrode, an Ag/AgCl electrode is used as a reference electrode, a platinum sheet electrode is used as an auxiliary electrode to form a three-electrode system, the electrode material for detecting ascorbic acid, the reference electrode and the auxiliary electrode are placed in the electrolyte, and cyclic voltammetry scanning is carried out on an electrochemical workstation;
wherein the electrolyte comprises a buffer solution with pH =7 and ascorbic acid, the buffer solution comprises CuH 2 PO 4 And CuHPO 4 Ascorbic acid was dissolved in a buffer solution and prepared to a concentration of 5X 10 -8 -5×10 -3 And mol/L of electrolyte.
The invention also protects the application of the biosensor in the determination of the ascorbic acid concentration.
Compared with the prior art, the invention has the beneficial effects that:
1. the method adopts an electrochemical method to detect the ascorbic acid, and has the advantages of high sensitivity, low detection limit, quick response time and good selectivity.
2. The method comprises the steps of firstly carrying out surface oxidation on foam nickel subjected to surface deoiling treatment by a hydrothermal oxidation method to prepare a Ni @ NiO composite material, wherein the Ni @ NiO composite material has the advantages of high chemical stability, good electrocatalysis performance, strong electron transfer capacity and the like, has potential application prospects in a biosensor, transferring an oxidized carbon nanotube to the surface of the Ni @ NiO composite material by an electro-spraying method, enabling the oxidized carbon nanotube to be stably positioned on the Ni @ NiO composite material to obtain the Ni @ NiO composite material/carbon nanotube, finally loading ascorbic acid oxidase on the surface of the Ni @ NiO composite material/carbon nanotube to prepare an electrode material of the ascorbic acid sensor, modifying the ascorbic acid oxidase to efficiently catalyze the oxidation of the ascorbic acid, enabling the electrode to have extremely high detection sensitivity, achieving extremely high detection limit and being suitable for trace detection of the ascorbic acid.
3. The carbon nano tube has the characteristics of small size, high mechanical strength, large specific surface area, high conductivity and strong interface effect, and has excellent electrical properties, so that the sensitivity and stability of biological detection can be improved, the overpotential of oxidation reduction is reduced, and direct electron transfer is carried out.
4. The invention takes foam nickel as a matrix, nickel (Ni) is a magnetic metal, has unique properties and can be applied to magnetic storage media, fuel cell electrodes, catalysts and the like, a NiO nano structure has the advantages of high chemical stability, good electrocatalysis performance, strong electron transfer capacity and the like, and has potential application prospects in biosensors, and the Ni @ NiO composite material has excellent electrochemical biological activity, magnetic performance and good dispersibility in water, so that the NiO nano structure has wide application prospects in the fields of bioseparation, biosensors and the like.
Drawings
FIG. 1 is a graph showing the circulation of the sensor electrode of the present invention in ascorbic acid solutions of different concentrationsCyclic voltammogram, in which: a. b, c, d, e and f represent ascorbic acid solutions having a concentration of 1X 10 -3 mol/L、1×10 -4 mol/L、1×10 - 5 mol/L、1×10 -6 mol/L、1×10 -7 mol/L、1×10 -8 ；
Fig. 2 is a performance test chart of charge and discharge of the electrode material for detecting ascorbic acid prepared by inventive example 2.
Detailed Description
The following description of the preferred embodiments 1-3 and the accompanying drawings 1-2 will be made in detail with reference to the accompanying drawings.
Example 1
A preparation method of an electrode material for detecting ascorbic acid is characterized by comprising the following steps:
(1) Preparation of Ni @ NiO composite:
cutting foamed nickel into 1 × 5cm 2 Soaking foamed nickel in acetone and performing ultrasonic treatment for 6 hours, then performing ultrasonic treatment for 2 hours in deionized water, then soaking the foamed nickel in 0.1mol/L HCl solution for 3 hours, finally washing with water for 2 hours and performing ultrasonic treatment, drying at normal temperature until the quality does not change any more, then placing the foamed nickel and the deionized water into a reaction kettle, immersing the foamed nickel in the deionized water, firstly reacting at 100 ℃ in the reaction kettle for 12 hours, then reacting at 140 ℃ for 12 hours, cooling to room temperature, then taking out and drying, finally sintering at 300 ℃ for 7 hours, and performing vacuum drying to prepare the Ni @ NiO composite material;
(2) Preparation of electrode material for detecting ascorbic acid:
dispersing 0.04g of carbon nano tube in concentrated nitric acid with volume fraction of 45%, refluxing for 12h at 100 ℃, cooling to room temperature, washing with deionized water until the deionized water is colorless and transparent, drying until the mass does not change any more, preparing oxidized carbon nano tube, ultrasonically dispersing the oxidized carbon nano tube in 20mL of N, N' -dimethylformamide to form dispersion liquid, injecting the dispersion liquid into an injection container with a spray head, electrifying, injecting the dispersion liquid in the injection container to the surface of the Ni @ NiO composite material through the spray head under the conditions that the temperature is 80 ℃, the distance between the surface of the Ni @ NiO composite material and the spray head is 10cm, the injection flow is 0.05mL/h, the voltage is 12kV, and the inner diameter of the spray head is 0.4mm, injecting the dispersion liquid to the surface of the Ni @ NiO composite material through the spray head, then carrying out heat treatment at 450 ℃ in an inert atmosphere for 10min to obtain the Ni @ NiO composite material/carbon nano tube, and then repeatedly dripping 3 times of the ascorbic acid oxidase solution on the Ni @ NiO composite material/carbon nano tube, wherein the preparation method of the ascorbic acid oxidase solution comprises the following steps: dissolving 500unit ascorbic acid oxidase in 100 mu L of chitosan acetic acid solution with the mass percentage concentration of 1%, drying at room temperature, and refrigerating at 2 ℃ to prepare the electrode material for detecting ascorbic acid.
Example 2
(1) Preparation of Ni @ NiO composite:
cutting foamed nickel into 2 × 4cm 2 Soaking foamed nickel in acetone and performing ultrasonic treatment for 7 hours, then washing in deionized water and performing ultrasonic treatment for 1.5 hours, then soaking the foamed nickel in 0.1mol/L HCl solution for ultrasonic treatment for 4 hours, finally washing with water for 1.5 hours and performing ultrasonic treatment, drying at normal temperature until the quality does not change any more, then putting the foamed nickel and the deionized water into a reaction kettle, immersing the foamed nickel in the deionized water, firstly reacting for 12 hours at 110 ℃ in the reaction kettle, then reacting for 12 hours at 135 ℃, cooling to room temperature, then taking out and drying, finally sintering for 6 hours at 350 ℃, and performing vacuum drying to prepare the Ni @ NiO composite material;
(2) Preparation of electrode material for detecting ascorbic acid:
dispersing 0.05g of carbon nano tubes in 45% of concentrated nitric acid by volume fraction, refluxing for 11h at 110 ℃, cooling to room temperature, washing with deionized water until the deionized water is colorless and transparent, drying until the mass does not change any more, preparing oxidized carbon nano tubes, ultrasonically dispersing the oxidized carbon nano tubes in 25mL of N-methylpyrrolidone to form a dispersion liquid, injecting the dispersion liquid into an injection container with a spray head, injecting the dispersion liquid in the injection container to the surface of a Ni @ NiO composite material at the temperature of 100 ℃ after electrifying, wherein the distance between the surface of the Ni @ NiO composite material and the spray head is 12cm, the injection flow is 0.08mL/h, the voltage is 10kV, and the inner diameter of the spray head is 0.5mm, injecting the dispersion liquid to the surface of the Ni @ NiO composite material through the spray head, then carrying out heat treatment at 350 ℃ for 15min in an inert atmosphere to obtain the Ni @ NiO composite material/carbon nano tubes, and then repeatedly dropwise adding a phosphate buffer solution of ascorbic acid oxidase on the Ni @ NiO composite material/carbon nano tubes for 4 times, wherein the preparation method of the phosphate buffer solution of the ascorbic acid oxidase is as follows: dissolving 500unit ascorbic acid oxidase in 100 mu L of chitosan acetic acid solution with the mass percentage concentration of 1%, drying at room temperature, and refrigerating at 4 ℃ to obtain the electrode material for detecting ascorbic acid.
Example 3
(1) Preparation of Ni @ NiO composite:
cutting foamed nickel into 3 × 3cm 2 Soaking foamed nickel in acetone and performing ultrasonic treatment for 8 hours, then washing in deionized water and performing ultrasonic treatment for 1 hour, then soaking the foamed nickel in 0.1mol/L HCl solution for ultrasonic treatment for 5 hours, finally washing with water for 1 hour and performing ultrasonic treatment, drying at normal temperature until the quality does not change any more, then placing the foamed nickel and the deionized water into a reaction kettle, immersing the foamed nickel in the deionized water, firstly reacting at 120 ℃ in the reaction kettle for 12 hours, then reacting at 130 ℃ for 12 hours, cooling to room temperature, then taking out and drying, finally sintering at 400 ℃ for 5 hours, and performing vacuum drying to prepare the Ni @ NiO composite material;
(2) Preparation of electrode material for detecting ascorbic acid:
dispersing 0.06g of carbon nano tube in concentrated nitric acid with volume fraction of 45%, refluxing for 10h at 120 ℃, cooling to room temperature, washing with deionized water until the deionized water is colorless and transparent, drying until the mass does not change any more, preparing oxidized carbon nano tube, ultrasonically dispersing the oxidized carbon nano tube in 30mL of N-methylpyrrolidone to form dispersion liquid, injecting the dispersion liquid into an injection container with a spray head, injecting the dispersion liquid in the injection container to the surface of a Ni @ NiO composite material at the temperature of 120 ℃ after electrifying, wherein the distance between the surface of the Ni @ NiO composite material and the spray head is 14cm, the injection flow is 0.1mL/h, the voltage is 8kV, and the inner diameter of the spray head is 0.6mm, injecting the dispersion liquid to the surface of the Ni @ NiO composite material through the spray head, then carrying out heat treatment for 20min at the temperature of 300 ℃ in an inert atmosphere to obtain the Ni @ NiO composite material/carbon nano tube, and then repeatedly dropwise adding a phosphate buffer solution of ascorbic acid oxidase on the Ni @ NiO composite material/carbon nano tube for 5 times, wherein the preparation method for preparing the phosphate buffer solution of the ascorbic acid oxidase is as follows: dissolving 500unit ascorbic acid oxidase in 100 mu L of chitosan acetic acid solution with the mass percentage concentration of 1%, drying at room temperature, and refrigerating at 2-6 ℃ to obtain the electrode material for detecting ascorbic acid.
The electrode materials for detecting ascorbic acid prepared in examples 1 to 3 of the present invention all have very high sensitivity and low detection limit, and have rapid response time, good stability and anti-interference performance, good selectivity, and comparable results, the present invention takes the electrode material for detecting ascorbic acid prepared in example 2 as an example, and takes the electrode material for detecting ascorbic acid prepared in example 2 as a working electrode, an Ag/AgCl electrode as a reference electrode, and a platinum sheet electrode as an auxiliary electrode to form a three-electrode system, and the electrode material, the reference electrode, and the auxiliary electrode for detecting ascorbic acid are placed in an electrolyte, and cyclic voltammetry scanning is performed on an electrochemical workstation, and the scanning results and conclusions are as follows:
(1) The research method comprises the following steps:
testing an instrument: CHI660E electrochemical workstation;
test 1: the electrode material for detecting ascorbic acid prepared in example 2 was used as a working electrode, an Ag/AgCl electrode was used as a reference electrode, a platinum sheet electrode was used as an auxiliary electrode to form a three-electrode system, and the electrode material for detecting ascorbic acid, the reference electrode and the auxiliary electrode were placed in an electrolyte solution containing a buffer solution of pH =7 and ascorbic acid, cuH 2 PO 4 And CuHPO 4 Is prepared by dissolving ascorbic acid in a buffer solution of (1) pH =7 to give concentrations of 5 × 10, respectively -8 -5×10 -3 Performing cyclic voltammetry scanning on an electrochemical workstation by using mol/L electrolyte;
as shown in FIG. 1, the current response of the biosensor prepared from the electrode material for detecting ascorbic acid to the ascorbic acid concentration detected by the biosensor is researched by cyclic voltammetry, and the ascorbic acid oxidase sensor has extremely high detection limit which can reach 1 × 10 at most -11 mol/L，At a concentration of 5X 10 respectively -8 -5×10 -3 The cyclic voltammogram scanned at 100mV in mol/L ascorbic acid solution showed that the oxidase sensor exhibited excellent electrocatalytic performance on ascorbic acid.
And (3) testing 2: the electrode material for detecting ascorbic acid prepared in example 2 is subjected to a charge and discharge performance test, as shown in fig. 2, so that after 10 charge and discharge cycles with a current density of 0.1C are performed, the stable capacity can reach 110mAh/g, and the stable capacity is linearly reduced under the condition that the number of cycles is gradually increased, and tends to be stable before 4 times, and the number of cycles is reduced at a very high speed after 4 times, so that the electrode material for detecting ascorbic acid prepared in the present invention can be measured on the same electrode for 4 times when the same ascorbic acid is measured, and the ascorbic acid oxidase solution is added dropwise again after ultrasonic cleaning and is detected.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (9)
1. An electrode material for detecting ascorbic acid, which is prepared by the following method: firstly, carrying out surface oxidation on foamed nickel subjected to surface deoiling treatment by a hydrothermal oxidation method to prepare a Ni @ NiO composite material, then modifying the surface of the oxidized carbon nano tube on the Ni @ NiO composite material by an electro-spraying method to obtain a Ni @ NiO composite material/carbon nano tube, and finally loading ascorbic acid oxidase on the surface of the Ni @ NiO composite material/carbon nano tube to prepare an electrode material for detecting ascorbic acid;
the electrode material for detecting ascorbic acid is prepared according to the following steps:
(1) Preparation of Ni @ NiO composite:
after degreasing the surface of the foamed nickel, carrying out surface oxidation by a hydrothermal oxidation method to prepare a Ni @ NiO composite material;
(2) Preparation of electrode material for detecting ascorbic acid: transferring the carbon oxide nanotube prepared by a chemical oxidation method to the surface of a Ni @ NiO composite material by adopting an electronic injection method, carrying out heat treatment to obtain a Ni @ NiO composite material/carbon nanotube, and fixing ascorbic acid oxidase on the surface of the Ni @ NiO composite material/carbon nanotube to prepare the electrode material for detecting ascorbic acid.
2. The electrode material for detecting ascorbic acid as claimed in claim 1, wherein the surface degreasing method in step (1) is: soaking foamed nickel in acetone and performing ultrasonic treatment, then performing ultrasonic treatment in deionized water, soaking the foamed nickel in an HCl solution with the concentration of 0.1mol/L and performing ultrasonic treatment, then performing ultrasonic treatment in the deionized water, and drying at normal temperature until the quality is not changed any more.
3. The electrode material for detecting ascorbic acid as claimed in claim 1, wherein the hydrothermal oxidation method of step (1) is: putting the foamed nickel and deionized water into a reaction kettle, reacting for 12 hours at 100-120 ℃ in the reaction kettle, then reacting for 12 hours at 130-140 ℃, cooling to room temperature, taking out and drying, finally sintering for 5-7 hours at 300-400 ℃, and drying in vacuum.
4. The electrode material for detecting ascorbic acid as claimed in claim 1, wherein the chemical oxidation method of the carbon nanotubes of step (2) is prepared by: adding concentrated nitric acid with volume fraction of 45% into carbon nano tube, refluxing at 100-120 deg.C for 10-12h, cooling to room temperature, washing with deionized water until deionized water is colorless and transparent, and oven drying until the mass is not changed.
5. The electrode material for detecting ascorbic acid as claimed in claim 1, wherein the operation of immobilizing ascorbate oxidase on the surface of ni @ nio composite/carbon nanotube in step (2) is: dripping ascorbic acid oxidase solution on the Ni @ NiO composite material/carbon nano tube repeatedly for 3-5 times, drying at normal temperature, and refrigerating at low temperature;
the preparation method of the ascorbic acid oxidase solution comprises the following steps: 500 units ascorbic acid oxidase is dissolved in 100 mul of chitosan acetic acid solution with the mass percent concentration of 1 percent.
6. The electrode material for detecting ascorbic acid as claimed in claim 1, wherein the electrospray method in step (2) is specifically operated as follows: ultrasonically dispersing carbon oxide nanotubes in N, N' -dimethylformamide or N-methylpyrrolidone to form a dispersion liquid, injecting the dispersion liquid into an injection container with a spray head, and injecting the dispersion liquid in the injection container to the surface of the Ni @ NiO composite material through the spray head after electrifying;
the electro-spraying method adopts the following spraying conditions: the temperature is 80-120 ℃, the distance between the surface of the Ni @ NiO composite material and the spray head is 10-14cm, the spray flow is 0.05-0.1mL/h, the voltage is 8-12kV, and the inner diameter of the spray head is 0.4-0.6mm.
7. The electrode material for detecting ascorbic acid as claimed in claim 1, wherein the heat treatment in the step (2) is performed by: treating at 300-450 deg.C for 10-20min in inert atmosphere.
8. The biosensor of the electrode material for detecting ascorbic acid as claimed in claim 1, wherein the biosensor comprises the electrode material for detecting ascorbic acid prepared in step (2), a reference electrode, an auxiliary electrode and an electrolyte, wherein the electrode material for detecting ascorbic acid is used as a working electrode, an Ag/AgCl electrode is used as a reference electrode, a platinum sheet electrode is used as an auxiliary electrode to form a three-electrode system, and the electrode material for detecting ascorbic acid, the reference electrode and the auxiliary electrode are placed in the electrolyte to perform cyclic voltammetry scanning on an electrochemical workstation;
wherein the electrolyte comprises a buffer solution with pH =7 and ascorbic acid, the buffer solution comprises CuH 2 PO 4 And CuHPO 4 Dissolving ascorbic acid in buffer solution and preparingPrepared to a concentration of 5X 10 -8 -5×10 -3 mol/L electrolyte.
9. Use of the biosensor of claim 8 in ascorbic acid concentration determination.
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