CN111363760A - 一种构建麦角硫因生产菌的方法 - Google Patents

一种构建麦角硫因生产菌的方法 Download PDF

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CN111363760A
CN111363760A CN202010194710.8A CN202010194710A CN111363760A CN 111363760 A CN111363760 A CN 111363760A CN 202010194710 A CN202010194710 A CN 202010194710A CN 111363760 A CN111363760 A CN 111363760A
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ergothioneine
gene expression
pgpd
plasmid
puc57
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Chinese (zh)
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范文超
高书良
王金刚
任亮
俞想
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Zhejiang Huarui Biotechnology Co ltd
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Zhejiang Huarui Biotechnology Co ltd
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Priority to CN202010194710.8A priority Critical patent/CN111363760A/zh
Priority to CN202010587928.XA priority patent/CN111534535B/zh
Publication of CN111363760A publication Critical patent/CN111363760A/zh
Priority to PCT/CN2021/077815 priority patent/WO2021185039A1/fr
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202010194710.8A 2020-03-19 2020-03-19 一种构建麦角硫因生产菌的方法 Pending CN111363760A (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202010194710.8A CN111363760A (zh) 2020-03-19 2020-03-19 一种构建麦角硫因生产菌的方法
CN202010587928.XA CN111534535B (zh) 2020-03-19 2020-06-24 一种构建麦角硫因生产菌的方法
PCT/CN2021/077815 WO2021185039A1 (fr) 2020-03-19 2021-02-25 Procédé de construction d'une souche produisant de l'ergothionéine

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CN202010194710.8A CN111363760A (zh) 2020-03-19 2020-03-19 一种构建麦角硫因生产菌的方法

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CN202010587928.XA Active CN111534535B (zh) 2020-03-19 2020-06-24 一种构建麦角硫因生产菌的方法

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021185039A1 (fr) * 2020-03-19 2021-09-23 浙江华睿生物技术有限公司 Procédé de construction d'une souche produisant de l'ergothionéine
CN114262702A (zh) * 2021-12-31 2022-04-01 西南大学 麦角硫因合成基因在谷氨酸棒杆菌中重建麦角硫因代谢途径中的应用及其方法
CN114277042A (zh) * 2021-12-29 2022-04-05 内蒙古金达威药业有限公司 一种高产麦角硫因的圆红冬孢酵母重组表达菌株及其构建方法和应用
CN114854660A (zh) * 2022-05-27 2022-08-05 深圳中科欣扬生物科技有限公司 一种高产麦角硫因的基因工程菌
CN114854659A (zh) * 2022-05-27 2022-08-05 深圳中科欣扬生物科技有限公司 一种麦角硫因生产工艺及其应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114107326A (zh) * 2020-09-01 2022-03-01 中国科学院微生物研究所 两个麦角硫因合成蛋白质及其在麦角硫因合成中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11390897B2 (en) * 2014-12-22 2022-07-19 National University Corporation Okayama University Method for producing ergothioneine
CN109401988B (zh) * 2018-11-27 2022-04-26 大连工业大学 一株圆红冬孢酵母及其在生产红酵母红素中的应用
CN110283856B (zh) * 2019-04-30 2020-06-26 弘恒泰(天津)科技发展有限公司 耐高温糙皮侧耳菌在生产麦角硫因中的应用
CN110358719B (zh) * 2019-07-23 2021-05-04 江南大学 一种发酵合成麦角硫因的工程菌株及其构建方法
CN110607286B (zh) * 2019-08-21 2021-02-19 华南农业大学 灰树花麦角硫因基因Gfegt1和Gfegt2在合成麦角硫因中的应用
CN110551697B (zh) * 2019-08-26 2021-02-19 华南农业大学 侧耳类食用菌麦角硫因合成酶pegt1和pegt2在合成麦角硫因中的应用
CN111363760A (zh) * 2020-03-19 2020-07-03 浙江华睿生物技术有限公司 一种构建麦角硫因生产菌的方法

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021185039A1 (fr) * 2020-03-19 2021-09-23 浙江华睿生物技术有限公司 Procédé de construction d'une souche produisant de l'ergothionéine
CN114277042A (zh) * 2021-12-29 2022-04-05 内蒙古金达威药业有限公司 一种高产麦角硫因的圆红冬孢酵母重组表达菌株及其构建方法和应用
CN114262702A (zh) * 2021-12-31 2022-04-01 西南大学 麦角硫因合成基因在谷氨酸棒杆菌中重建麦角硫因代谢途径中的应用及其方法
CN114262702B (zh) * 2021-12-31 2023-12-08 西南大学 麦角硫因合成基因在谷氨酸棒杆菌中重建麦角硫因代谢途径中的应用及其方法
CN114854660A (zh) * 2022-05-27 2022-08-05 深圳中科欣扬生物科技有限公司 一种高产麦角硫因的基因工程菌
CN114854659A (zh) * 2022-05-27 2022-08-05 深圳中科欣扬生物科技有限公司 一种麦角硫因生产工艺及其应用
CN114854659B (zh) * 2022-05-27 2024-03-26 深圳中科欣扬生物科技有限公司 一种麦角硫因生产工艺及其应用
CN114854660B (zh) * 2022-05-27 2024-03-26 深圳中科欣扬生物科技有限公司 一种高产麦角硫因的基因工程菌

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CN111534535B (zh) 2022-02-22
WO2021185039A1 (fr) 2021-09-23
CN111534535A (zh) 2020-08-14

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