CN111348958A - Fertilizer synergist containing chitin oligosaccharide and application thereof - Google Patents
Fertilizer synergist containing chitin oligosaccharide and application thereof Download PDFInfo
- Publication number
- CN111348958A CN111348958A CN202010177249.5A CN202010177249A CN111348958A CN 111348958 A CN111348958 A CN 111348958A CN 202010177249 A CN202010177249 A CN 202010177249A CN 111348958 A CN111348958 A CN 111348958A
- Authority
- CN
- China
- Prior art keywords
- fertilizer
- chitosan oligosaccharide
- nitrogen
- improving
- utilization rate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H5/00—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium
- C07H5/04—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium to nitrogen
- C07H5/06—Aminosugars
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Materials Engineering (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a fertilizer synergist containing chitooligosaccharide and/or chitooligosaccharide salt and application thereof, wherein the chitooligosaccharide is an oligomer formed by connecting glucosamine (less than or equal to 85%) and acetylglucosamine (more than or equal to 15%) through β -1, 4-glycosidic bond, the molecular weight is more than 300Da and less than 5000 Da.
Description
Technical Field
The invention belongs to the field of fertilizer synergists, and particularly relates to a fertilizer synergist containing chitosan oligosaccharide, a fertilizer composition and application of the fertilizer synergist and the fertilizer composition in improving the utilization rate of a fertilizer.
Background
The use of the fertilizer is an essential measure for ensuring the crop yield and the grain safety. However, in recent years, excessive input of fertilizers is found, the yield increasing effect of the fertilizers cannot be further improved, and a series of problems of low fertilizer utilization rate, excessive accumulation of inorganic nutrients in soil, hardening and acidification of furniture soil and the like are caused. Therefore, how to improve and promote the utilization rate of the fertilizer and reduce the excessive accumulation of inorganic nutrients in the soil is a key problem for solving the problems of reducing the fertilizer input, improving the soil environment and ensuring the crop yield. The addition of fertilizer synergist into fertilizer is an important measure for increasing the utilization rate of fertilizer. For example, urease inhibitors and nitrification inhibitors have significant effects on increasing the availability of nitrogen in macroelement fertilizers. However, the fertilizer synergist has a single effect, can only have a remarkable effect on the synergism of certain elements, and has environmental safety hazards. Therefore, there is an urgent need for some fertilizer synergists that have the biological safety of increasing the availability of various elements in the fertilizer.
Chitosan oligosaccharide is an oligomer formed by connecting glucosamine and acetylglucosamine through β -1, 4-glycosidic bonds, and although the chitosan oligosaccharide with a specific structure can induce plant disease resistance (PNAS, 2014, E404-E413), induce plant to generate insect resistance activity (Chinese patent invention: 201810531128.9) and promote hemp growth (Chinese patent invention: 201811607504.4), the research on agriculture is just started, and the application of the chitosan oligosaccharide needs to be further discussed.
Disclosure of Invention
In order to solve the technical problems, the invention provides a fertilizer synergist containing chitosan oligosaccharide for improving the utilization rate of a fertilizer.
The technical scheme of the invention is as follows:
according to the first aspect, the invention provides a fertilizer synergist, which contains chitosan oligosaccharide, wherein the chitosan oligosaccharide is an oligomer formed by connecting glucosamine and acetylglucosamine through β -1, 4-glycosidic bonds.
The chitooligosaccharides as used herein include chitooligosaccharides present in various salt forms, such as chitooligosaccharide acetate, chitooligosaccharide hydrochloride, chitooligosaccharide phosphate, chitooligosaccharide sulfate, chitooligosaccharide lactate, and the like.
Preferably, the proportion of the acetylglucosamine units is greater than or equal to 15%, and the proportion of the glucosamine units is less than or equal to 85%.
More preferably, the chitosan oligosaccharide has a proportion of acetylglucosamine units of 35% or more and a proportion of glucosamine units of 65% or less.
Preferably, the molecular weight of the chitosan oligosaccharide is more than 300Da and less than 5000 Da.
As a further preference, the molecular weight of the chitin oligosaccharide is greater than 400Da and less than 3000 Da.
In a second aspect, the invention provides a preparation method of the above chitosan oligosaccharide, wherein the preparation method is an enzyme degradation method, an acetylation method or a chitin degradation method;
the enzyme degradation method comprises the following steps: hydrolyzing chitosan by chitinase, chitosanase or non-specific enzymes having chitosan hydrolyzing activity to obtain the chitooligosaccharide;
the acetylation method comprises the following steps: adding an acetylation reagent into a solution taking chitosan oligosaccharide as a substrate to perform acetylation reaction to obtain the chitosan oligosaccharide;
the chitin degradation method comprises the following steps: the chitosan oligosaccharide is obtained by using chitin as a substrate through acid hydrolysis, microwave degradation or superfine grinding.
In a third aspect, the invention provides an application of a fertilizer synergist, wherein the fertilizer synergist is used for improving the absorption rate of nutrient elements in a macroelement fertilizer, a medium element fertilizer and a trace element fertilizer.
Macroelement fertilizer refers to that the content of nutrient elements of nitrogen, phosphorus and potassium in the dry matter component of crops is more than one percent, and is called macroelement fertilizer, and the fertilizer containing macroelement is called macroelement fertilizer.
The medium element fertilizer refers to the fertilizer containing one to ten thousandth of nutrient elements of calcium, magnesium and sulfur in the dry matter component of crops, and is called medium element fertilizer.
The microelements comprise boron, zinc, molybdenum, ferrum, manganese, copper and other nutrient elements.
Preferably, the fertilizer synergist is used to increase the availability of nitrogen in a macroelement fertilizer; or used for improving the utilization rate of calcium in the secondary element fertilizer; or used for improving the utilization rate of boron in the trace element fertilizer.
Preferably, the fertilizer synergist is used for improving the utilization rate of nitrogen in a macroelement fertilizer for rice; or used for improving the utilization rate of calcium in the medium element fertilizer for the apple trees; or used for improving the utilization rate of the peanuts to the boron in the trace element fertilizer.
In a fourth aspect, the invention provides an agricultural fertilizer composition, which contains nutrient elements and the fertilizer synergist.
Preferably, the nutrient elements are any one or more of nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, iron and copper; the mass fraction of the chitosan oligosaccharide in the fertilizer composition is 0.1-10%.
In a fifth aspect, the present invention provides a method for using a fertilizer composition, the method comprising: the fertilizer composition is used for broadcasting and applying 5kg-100 kg/mu; or diluted 100-fold and 5000-fold spraying; or diluting 100-; or 100 times diluted and 5000 times drip irrigation.
Advantageous effects
1. The invention provides a fertilizer synergist containing chitosan oligosaccharide, which can improve the utilization rate of nutrient elements in a fertilizer, reduce the use of chemical fertilizers and reduce the accumulation of inorganic nutrient elements in soil, thereby improving the soil environment.
2. The fertilizer synergist containing the chitosan oligosaccharide can improve the utilization rate of various elements in the fertilizer, and particularly can promote plants such as peanuts, apples or rice to absorb and utilize elements such as nitrogen, calcium, boron and the like in the fertilizer, so that the crop yield is improved.
3. The fertilizer synergist containing the chitosan oligosaccharide can reduce the production cost by reducing the use of chemical fertilizers.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1: preparation of chitin oligose by enzyme degradation method
1kg of chitosan having a degree of deacetylation of 60% was weighed as a substrate, and added to a constant temperature reaction vessel containing 20L of an aqueous solution of acetic acid having a content of 1.5% (m/v), and sufficiently stirred to be completely dissolved. Regulating the reaction temperature to 40 ℃, adding 10000U chitosanase, and reacting at constant temperature for 48 hours. After the reaction is finished, insoluble substances are filtered by a microfiltration device, and then moisture is removed by a spray drying device, so that the chitooligosaccharide (NA-COS 1) with the acetylglucosamine unit ratio of about 40 percent and the glucosamine unit ratio of about 60 percent in the sugar chain is prepared, and the molecular weight is distributed between 300Da and 2000 Da.
Example 2: preparation of chitin oligosaccharide by acetylation
1kg of chitosan oligosaccharide (molecular weight of 300-2000Da, degree of deacetylation of 90%) was dissolved in 10L of water, 600mL of methanol was added, 20g of 4-Dimethylaminopyridine (DMAP) was added, 900mL of acetic acid was added, acetylation reaction was performed at 80 ℃ for 6 hours, and then moisture was removed by a spray drying apparatus to obtain chitosan oligosaccharide (NA-COS 2). Calculated by nuclear magnetic resonance spectrum, the acetyl degree is 87%, and the hydroxyl group is not reacted, namely the proportion of acetylglucosamine units in the sugar chain is about 87%, and the proportion of glucosamine units in the sugar chain is about 13%. The molecular weight distribution is 300Da-2000 Da.
1. Effect of Chitosan oligosaccharide on utilization rate of nitrogen in fertilizer
The chitin oligosaccharide prepared in the examples 1 and 2 is used as a fertilizer synergist of a macroelement fertilizer, the tested crop is rice, 6 different tests are set, and the experimental scheme is shown in the following table 1. Wherein chitosan oligosaccharide (COS, deacetylation degree greater than 90%, available from Glare Biotech Co., Ltd., Dalian province) is used as control fertilizer synergist. The nitrogen fertilizer, the phosphorus fertilizer and the potassium fertilizer are respectively urea, calcium superphosphate and potassium chloride. All the nitrogenous fertilizers and potash fertilizers subjected to fertilization treatment are applied in two times: 50 percent of the fertilizer is used as a base fertilizer and 50 percent of the fertilizer is used as a tillering fertilizer; the phosphate fertilizer is used as a base fertilizer for one-time application.
TABLE 1 Experimental protocols for Rice
Each experiment was repeated 3 times with a cell area of 20m2And randomly arranging the blocks, and performing independent irrigation and drainage. And (4) beating and drying the rice in the sun for each cell, weighing the obtained product, and collecting plant samples to determine the nitrogen content of the rice and the straws. Collecting plough layer soil samples of 0-20cm for measuring nitrate nitrogen, ammonium nitrogen and microbial biomass nitrogen, and measuring soil volume weight by adopting a cutting ring method to extract plough layer soil. The content of nitrogen in the paddy rice and the straw is measured by adopting a method for measuring nitrogen, phosphorus and potassium in NY/T2017-. Soil ammonium nitrogen and nitrate nitrogen are leached by 1mol/L KCl solution and then are measured by a flow analyzer; fumigating-K for soil microbial biomass nitrogen by adopting chloroform2SO4Solution leaching method.
TABLE 2 Rice yield, Nitrogen content and Nitrogen uptake for different treatment regimes
The significance test of the difference is carried out by a Duncan new double-pole difference method, and the difference is expressed by different letters after the same column of data to reach the significance level of 0.05.
The results of comparison example 3 with examples 3 and 4 in table 2 show that the addition of two kinds of chitooligosaccharides to the fertilizer can increase the yield of rice.
The fertilizer in example 3 is added with NA-COS1, so that the effect of improving the nitrogen absorption of rice plants is most obvious, the nitrogen absorption is improved by 9.3% compared with the group 2 in the comparative example, and the nitrogen absorption is improved by 16.2% compared with the group 3 in the comparative example. The above results show that the rice yield is still higher than that of the normal nitrogen application group (comparative example 2) with a 20% reduction in nitrogen application amount after adding NA-COS1 to the fertilizer.
As can be seen from the comparison of the data in examples 3 and 4 with the data in comparative example 4, the chitosan oligosaccharide is superior to chitosan oligosaccharide in the aspects of improving the nitrogen absorption of rice plants and improving the rice yield.
Looking at the effect of nitrogen fertilizer application amount on nitrogen use efficiency from comparative example 2 and comparative example 3 (table 3), we found that nitrogen absorption efficiency could be significantly improved after reducing nitrogen application amount, and that in the case of reducing 20% of nitrogen fertilizer application amount, after further adding two kinds of chitooligosaccharides NA-COS1 and NA-COS2 (examples 3 and 4), nitrogen absorption rate was increased by 16.2% and 5.7%, respectively, indicating that chitooligosaccharide could improve nitrogen absorption rate of rice to fertilizer, wherein (nitrogen fertilizer apparent utilization ratio (%) (nitrogen absorption amount on the ground in nitrogen application region-nitrogen absorption amount on the ground in control region)/nitrogen application amount ×, nitrogen fertilizer agronomy efficiency (kg/kg) (rice yield in nitrogen application region-rice yield in control region)/nitrogen application amount), partial productivity (kg/kg) (% nitrogen application region/nitrogen application amount), plant nitrogen application region nitrogen application efficiency (%) (nitrogen application amount on the ground/nitrogen application region/nitrogen application amount ×.)
TABLE 3 Nitrogen utilization efficiency for different treatments
The significance test of the difference is carried out by a Duncan new double-pole difference method, and the difference is expressed by different letters after the same column of data to reach the significance level of 0.05.
The effect of adding chitooligosaccharide to the fertilizer on the nitrogen content of the harvested rice soil was observed from table 4. The results show that after two kinds of chitosan oligosaccharide NA-COS1 and NA-COS2 are added into the fertilizer, the content of inorganic nitrogen (nitrate N and ammonium N) in soil can be obviously reduced, and the content of organic nitrogen of soil microorganisms can be increased.
TABLE 4 Nitrogen content in post-harvest differently treated soils
| Test of | Variables of | Nitrate state N | In the ammonium state N | Soil microbial biomass N |
| COMPARATIVE EXAMPLE 1(CK1) | Does not need to be fertilized | 1.92c | 16.5b | 60.6e |
| COMPARATIVE EXAMPLE 2(CK2) | 100％N | 3.3a | 18.2a | 68.1c |
| COMPARATIVE EXAMPLE 3(CK3) | 80％N | 2.82b | 14.1d | 65.7d |
| COMPARATIVE EXAMPLE 4(CK4) | 80％N+COS | 1.91c | 14.6cd | 74.1b |
| Example 3 | 80％N+NA-COS1 | 1.89cd | 12.7e | 79.3a |
| Example 4 | 80％N+NA-COS2 | 1.75d | 15.2c | 75.2b |
The significance test of the difference is carried out by a Duncan new double-pole difference method, and the difference is expressed by different letters after the same column of data to reach the significance level of 0.05.
In conclusion, the data of example 3 show that, in the rice planting process, the chitosan oligosaccharide used as the fertilizer synergist can improve the nitrogen absorption rate in the fertilizer, reduce the use of chemical fertilizer, reduce the accumulation of inorganic nutrient elements in soil, and improve the rice yield.
2. Influence of chitin oligosaccharide on utilization rate of calcium in fertilizer
The chitosan oligosaccharide prepared in the examples 1 and 2 is used as a fertilizer synergist of a secondary element fertilizer, and 36 apple trees with good growth condition, normal results and basically consistent tree vigor are selected for a test crop, namely 7-year-old apples. The experiment has 5 schemes in total, and the specific scheme is as follows:
1) diluting the medium element water-soluble fertilizer (the calcium concentration reaches 100g/L) by 1000 times and spraying;
2) the medium element water soluble fertilizer (calcium concentration reaches 100g/L) +50g/L COS is diluted by 1000 times and sprayed;
3) the medium element water-soluble fertilizer (calcium concentration reaches 100g/L) +50g/L NA-COS1, diluted 1000 times and sprayed;
4) the medium element water-soluble fertilizer (calcium concentration reaches 100g/L) +50g/L NA-COS2, diluted 1000 times and sprayed;
5) and (5) spraying clear water.
Reference is made to comparative example 5, comparative example 6, example 5, example 6, comparative example 7, respectively. Random block trial design, 3 replicates, 3 of which were one cell. Spraying after flowering, wherein the spraying is carried out once every 10 days, and the spraying is carried out 3 times in total. The calcium content of the fruits in the young fruit stage and the mature stage was measured, respectively, and the results are shown in Table 5. The pulp calcium content adopts HNO3-HClO4(4:1) digestion-atomic absorption spectrophotometry.
TABLE 5 Effect of Chitosan oligosaccharide on increasing the utilization of calcium in fertilizers
| Test of | Calcium content in young fruit (mg/g) | Calcium content (mg/g) in ripe fruit |
| Comparative example 5 | 0.52b | 0.35b |
| Comparative example 6 | 0.54b | 0.37b |
| Example 5 | 0.58a | 0.41a |
| Example 6 | 0.57ab | 0.42a |
| Comparative example 7 | 0.49c | 0.31c |
The significance test of the difference is carried out by a Duncan new double-pole difference method, and the difference is expressed by different letters after the same column of data to reach the significance level of 0.05.
As shown in the test data in the table 5, the chitosan oligosaccharides NA-COS1 and NA-COS2 with two structures can obviously improve the absorption of calcium in the middle element water-soluble fertilizer by apple tree fruits, and the effect of the chitosan oligosaccharide is superior to that of chitosan oligosaccharide.
3. Influence of chitooligosaccharide on utilization rate of boron in fertilizer
The chitooligosaccharide prepared in the example 1 and the example 2 is used as a fertilizer synergist of a trace element water-soluble fertilizer, and the crop to be tested is peanut. 8 schemes are designed in the test, and the specific scheme is as follows:
1) diluting the trace element water-soluble fertilizer (the boron concentration is more than 100g/L) by 1000 times and spraying;
2) diluting 1000 times of water-soluble trace element fertilizer (boron concentration is more than 100g/L) and 50g/L COS for spraying;
3) diluting 1000 times of water-soluble trace element fertilizer (boron concentration is more than 100g/L) and 50g/L of NA-COS1, and spraying;
4) diluting 1000 times of water-soluble trace element fertilizer (boron concentration is more than 100g/L) and 50g/L of NA-COS2, and spraying;
5) the trace element water soluble fertilizer (boron concentration is more than 100g/L) +20g/L COS is diluted 1000 times and sprayed
6) Diluting 1000 times of water-soluble trace element fertilizer (boron concentration is more than 100g/L) and 20g/L of NA-COS1, and spraying;
7) diluting 1000 times of water-soluble trace element fertilizer (boron concentration is more than 100g/L) and 20g/L of NA-COS2, and spraying;
8) and (5) spraying clear water.
Reference is made to comparative example 8, comparative example 9, example 7, example 8, comparative example 10, example 9, example 10, comparative example 11, respectively. Spraying in the seedling stage of the peanut, collecting stems and leaves 24 hours after spraying, and measuring the boron content in the stems and leaves by adopting an Optima 2100DV plasma emission spectrometer.
TABLE 6 Effect of chitooligosaccharides on increasing the utilization of boron in fertilizers
The significance test of the difference is carried out by a Duncan new double-pole difference method, and the difference is expressed by different letters after the same column of data to reach the significance level of 0.05.
As can be seen from the test data in Table 6, the addition of chitosan oligosaccharide and chitosan oligosaccharides NA-COS1 and NA-COS2 with two structures can obviously improve the absorption of the peanut to boron in the trace element water-soluble fertilizer. The chitosan oligosaccharide NA-COS1 and NA-COS2 with two structures have higher absorption and utilization rate of boron than chitosan oligosaccharide. The most remarkable effect of improving the absorption of the peanuts to boron is achieved by adding 20g/L of NA-COS2 into the boron-containing trace element water-soluble fertilizer.
Claims (10)
1. The fertilizer synergist is characterized by comprising chitosan oligosaccharide, wherein the chitosan oligosaccharide is an oligomer formed by connecting glucosamine and acetylglucosamine through β -1, 4-glycosidic bonds.
2. The fertilizer synergist of claim 1, wherein: in the chitosan oligosaccharide, the proportion of the acetylglucosamine unit is more than or equal to 15 percent, and the proportion of the glucosamine unit is less than or equal to 85 percent; the molecular weight of the chitosan oligosaccharide is more than 300Da and less than 5000 Da.
3. The fertilizer synergist of claim 2, wherein: in the chitosan oligosaccharide, the proportion of the acetylglucosamine unit is more than or equal to 35 percent, and the proportion of the glucosamine unit is less than or equal to 65 percent; the molecular weight of the chitosan oligosaccharide is more than 400Da and less than 3000 Da.
4. A method for preparing chitooligosaccharide of claim 1-3, wherein the method comprises the following steps: the preparation method is an enzyme degradation method, an acetylation method or a chemical physical degradation method;
the enzyme degradation method comprises the following steps: hydrolyzing chitosan by chitinase, chitosanase or non-specific enzymes having chitosan hydrolyzing activity to obtain the chitooligosaccharide;
the acetylation method comprises the following steps: adding an acetylation reagent into a solution taking chitosan oligosaccharide as a substrate to perform acetylation reaction to obtain the chitosan oligosaccharide;
the chemical physical degradation method comprises the following steps: the chitosan oligosaccharide is obtained by using chitin as a substrate through acid hydrolysis, microwave degradation or superfine grinding.
5. Use of a fertilizer synergist according to claims 1-3, characterized in that: the fertilizer synergist is used for improving the absorption rate of nutrient elements in macroelement fertilizers, medium element fertilizers and trace element fertilizers.
6. Use of a fertilizer synergist according to claim 5, characterized in that: the fertilizer synergist is used for improving the utilization rate of nitrogen in a macroelement fertilizer; or used for improving the utilization rate of calcium in the secondary element fertilizer; or used for improving the utilization rate of boron in the trace element fertilizer.
7. Use of a fertilizer synergist according to claim 6, characterized in that: the fertilizer synergist is used for improving the utilization rate of nitrogen in the rice to the macroelement fertilizer; or used for improving the utilization rate of calcium in the medium element fertilizer for the apple trees; or used for improving the utilization rate of the peanuts to the boron in the trace element fertilizer.
8. An agricultural fertilizer composition characterized by: the fertilizer composition comprises nutrient elements and the fertilizer synergist of any one of claims 1-3.
9. The fertilizer composition of claim 8, wherein: the nutrient elements are any one or more of nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, iron and copper; the mass fraction of the chitosan oligosaccharide in the fertilizer composition is 0.1-10%.
10. A method of using the fertilizer composition of claims 8-9, wherein: the fertilizer composition is used for broadcasting and applying 5kg-100 kg/mu; or diluted 100-fold and 5000-fold spraying; or diluting 100-; or 100 times diluted and 5000 times drip irrigation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010177249.5A CN111348958A (en) | 2020-03-13 | 2020-03-13 | Fertilizer synergist containing chitin oligosaccharide and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010177249.5A CN111348958A (en) | 2020-03-13 | 2020-03-13 | Fertilizer synergist containing chitin oligosaccharide and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111348958A true CN111348958A (en) | 2020-06-30 |
Family
ID=71190746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010177249.5A Pending CN111348958A (en) | 2020-03-13 | 2020-03-13 | Fertilizer synergist containing chitin oligosaccharide and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111348958A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773131A (en) * | 2021-07-05 | 2021-12-10 | 河南正达作物保护有限公司 | Preparation and application method of fertilizer synergist |
| US11639319B2 (en) * | 2020-08-27 | 2023-05-02 | Instituto De Ecologia, A.C. | Particles of fertilizers encapsulated in modified chitosan and obtaining method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674968A (en) * | 2011-09-27 | 2012-09-19 | 宁夏天瑞产业集团现代农业有限公司 | Drop irrigation fertilizer for selenium sand melons |
| CN103204729A (en) * | 2012-01-17 | 2013-07-17 | 山东新沭化化肥有限公司 | Composite fertilizer comprising chitin, humic acid, and middle and trace elements |
| CN104692932A (en) * | 2015-02-12 | 2015-06-10 | 海南正业中农高科股份有限公司 | Synergistic composition containing chitosan oligosaccharide and amino acid |
| CN104961613A (en) * | 2015-07-13 | 2015-10-07 | 青岛苏贝尔作物营养有限公司 | Potassium nitrate-containing water soluble fertilizer and preparation method thereof |
| CN105175049A (en) * | 2015-07-13 | 2015-12-23 | 青岛苏贝尔作物营养有限公司 | Leaf fertilizer containing calcium ammonium nitrate, and preparation method thereof |
| CN107162751A (en) * | 2017-06-14 | 2017-09-15 | 芜湖飞高农业有限公司 | A kind of jujube plants dedicated fertilizer |
| CN109279956A (en) * | 2018-10-24 | 2019-01-29 | 江苏辉丰生物技术有限公司 | Fertilizer composition containing amino-oligosaccharide and sulfuric acid K-Mg-Ca |
| CN109438103A (en) * | 2019-01-10 | 2019-03-08 | 江苏心实肥业集团有限公司 | A kind of chitosan oligosaccharide crop nutrition spray fertilizer and preparation method thereof |
-
2020
- 2020-03-13 CN CN202010177249.5A patent/CN111348958A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674968A (en) * | 2011-09-27 | 2012-09-19 | 宁夏天瑞产业集团现代农业有限公司 | Drop irrigation fertilizer for selenium sand melons |
| CN103204729A (en) * | 2012-01-17 | 2013-07-17 | 山东新沭化化肥有限公司 | Composite fertilizer comprising chitin, humic acid, and middle and trace elements |
| CN104692932A (en) * | 2015-02-12 | 2015-06-10 | 海南正业中农高科股份有限公司 | Synergistic composition containing chitosan oligosaccharide and amino acid |
| CN104961613A (en) * | 2015-07-13 | 2015-10-07 | 青岛苏贝尔作物营养有限公司 | Potassium nitrate-containing water soluble fertilizer and preparation method thereof |
| CN105175049A (en) * | 2015-07-13 | 2015-12-23 | 青岛苏贝尔作物营养有限公司 | Leaf fertilizer containing calcium ammonium nitrate, and preparation method thereof |
| CN107162751A (en) * | 2017-06-14 | 2017-09-15 | 芜湖飞高农业有限公司 | A kind of jujube plants dedicated fertilizer |
| CN109279956A (en) * | 2018-10-24 | 2019-01-29 | 江苏辉丰生物技术有限公司 | Fertilizer composition containing amino-oligosaccharide and sulfuric acid K-Mg-Ca |
| CN109438103A (en) * | 2019-01-10 | 2019-03-08 | 江苏心实肥业集团有限公司 | A kind of chitosan oligosaccharide crop nutrition spray fertilizer and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| 任梦娟等: "减氮条件下喷施壳寡糖对烤烟氮素利用率及烟叶品质的影响", 《烟草科技》 * |
| 刘夏石: "《计算施肥学与理性农业的探索》", 30 November 2003, 东南大学出版社 * |
| 王倬等: "几丁类寡糖构效关系及植物免疫诱导活性研究进展", 《农药科学与管理》 * |
| 谢卫国: "《测土配方施肥理论与实践》", 31 May 2006, 湖南科学技术出版社 * |
| 邓华凤: "《杂交水稻知识大全》", 30 September 2014, 中国科学技术出版社 * |
| 钱成洋等: "一种含壳寡糖增效剂的新型肥料", 《磷肥与复肥》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11639319B2 (en) * | 2020-08-27 | 2023-05-02 | Instituto De Ecologia, A.C. | Particles of fertilizers encapsulated in modified chitosan and obtaining method |
| CN113773131A (en) * | 2021-07-05 | 2021-12-10 | 河南正达作物保护有限公司 | Preparation and application method of fertilizer synergist |
| CN113773131B (en) * | 2021-07-05 | 2022-08-05 | 河南正达作物保护有限公司 | Preparation and application method of fertilizer synergist |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102584448B (en) | Compound fertilizer | |
| CN111777446A (en) | Mineral source potassium fulvate water-soluble fertilizer and preparation method thereof | |
| CN102924182B (en) | Environment-friendly soil-improving liquid fertilizer and preparing method thereof | |
| CN108271787A (en) | A kind of compound oligosaccharide fruits and vegetables support the preparation method of root element | |
| CN111348958A (en) | Fertilizer synergist containing chitin oligosaccharide and application thereof | |
| CN111995463A (en) | Spraying production process of mineral source potassium fulvate compound fertilizer | |
| CN108329157A (en) | Nutrition-balanced instant water-soluble fertilizer powder of one kind and preparation method thereof | |
| CN116444309A (en) | Microbial organic seaweed fertilizer and preparation method thereof | |
| CN113929532A (en) | Brown algae oligosaccharide water-soluble fertilizer and preparation method thereof | |
| CN112010703A (en) | Biological humic acid fertilizer and preparation method thereof | |
| CN111423279A (en) | Composite biological fertilizer for grain crops and preparation method thereof | |
| CN116730764A (en) | Fertilizer for improving soil environment and preparation method thereof | |
| CN113087568B (en) | Amino acid water-soluble fertilizer | |
| CN112028690B (en) | Slow-release synergistic phosphate fertilizer and preparation method and application thereof | |
| CN105237277B (en) | A kind of multi-functional boron magnesium zinc humic acid chelating Eco-fertilizer | |
| Pešaković et al. | Beneficial Role of Biofertilization on Yield Related Characteristics of Two Apple Cultivars and Soil Microorganisms under Orchard Conditions | |
| CN108546179A (en) | Promoting root growth, anti-lopsided block root crop fertilizer | |
| CN115322027B (en) | Special water-soluble compound fertilizer for kiwi fruits and fertilizing method thereof | |
| CN114213176B (en) | Fertilizer for improving stress resistance and quality of crops and preparation method thereof | |
| KR100838048B1 (en) | Application method for nutrition of nature plant with isolated phenolic acid from oak wood | |
| CN113880640A (en) | Water-soluble silicon fertilizer and preparation process and application thereof | |
| CN116813410A (en) | Crop root system nutrient absorption growth promoter and preparation method and application thereof | |
| CN116621633A (en) | Water-retention slow-release fertilizer special for corn and preparation method thereof | |
| CN115720796A (en) | Method for planting medlar by utilizing bio-based hydrogel | |
| CN112480183A (en) | Preparation method of mineral source potassium fulvate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200630 |
|
| RJ01 | Rejection of invention patent application after publication |